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Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
 
BMC Genomics. 2009; 10: 386.
Published online Aug 20, 2009. doi:  10.1186/1471-2164-10-386
PMCID: PMC2743715
Generation, annotation, and analysis of ESTs from midgut tissue of adult female Anopheles stephensi mosquitoes
Deepak P Patil,corresponding author1 Santosh Atanur,corresponding author2 Dhiraj P Dhotre,corresponding author1 D Anantharam,corresponding author1 Vineet S Mahajan,1 Sandeep A Walujkar,1 Rakesh K Chandode,1 Girish J Kulkarni,1 Pankaj S Ghate,1 Abhishek Srivastav,1 Kannayakanahalli M Dayananda,1 Neha Gupta,1 Bhakti Bhagwat,2 Rajendra R Joshi,2 Devendra T Mourya,3 Milind S Patole,1 and Yogesh S Shouchecorresponding author1
1Lab 3, National Center for Cell Science, Pune – 411007, India
2Bioinformatics Team, Center for Development of Advanced Computing, Pune University Campus, Pune – 411007, India
3Microbial Containment Complex, National Institute of Virology, Pune – 411007, India
corresponding authorCorresponding author.
Deepak P Patil: deepakpatilp/at/gmail.com; Santosh Atanur: santoshatanur/at/gmail.com; Dhiraj P Dhotre: dheerajdhotre/at/gmail.com; D Anantharam: nirvikalpa/at/rediffmail.com; Vineet S Mahajan: vineetsm2002/at/gmail.com; Sandeep A Walujkar: sandeepwalujkar/at/gmail.com; Rakesh K Chandode: rchandode/at/gmail.com; Girish J Kulkarni: girishmbu/at/gmail.com; Pankaj S Ghate: ghate.pankaj/at/gmail.com; Abhishek Srivastav: seqdgene/at/gmail.com; Kannayakanahalli M Dayananda: kmd1234/at/gmail.com; Neha Gupta: nehagupta279/at/gmail.com; Bhakti Bhagwat: bhaktib/at/cdac.in; Rajendra R Joshi: rajendra/at/cdac.in; Devendra T Mourya: mouryadt/at/vsnl.net; Milind S Patole: patole/at/nccs.res.in; Yogesh S Shouche: yogesh/at/nccs.res.in
Received September 16, 2008; Accepted August 20, 2009.
Abstract
Background
Malaria is a tropical disease caused by protozoan parasite, Plasmodium, which is transmitted to humans by various species of female anopheline mosquitoes. Anopheles stephensi is one such major malaria vector in urban parts of the Indian subcontinent. Unlike Anopheles gambiae, an African malaria vector, transcriptome of A. stephensi midgut tissue is less explored. We have therefore carried out generation, annotation, and analysis of expressed sequence tags from sugar-fed and Plasmodium yoelii infected blood-fed (post 24 h) adult female A. stephensi midgut tissue.
Results
We obtained 7061 and 8306 ESTs from the sugar-fed and P. yoelii infected mosquito midgut tissue libraries, respectively. ESTs from the combined dataset formed 1319 contigs and 2627 singlets, totaling to 3946 unique transcripts. Putative functions were assigned to 1615 (40.9%) transcripts using BLASTX against UniProtKB database. Amongst unannotated transcripts, we identified 1513 putative novel transcripts and 818 potential untranslated regions (UTRs). Statistical comparison of annotated and unannotated ESTs from the two libraries identified 119 differentially regulated genes. Out of 3946 unique transcripts, only 1387 transcripts were mapped on the A. gambiae genome. These also included 189 novel transcripts, which were mapped to the unannotated regions of the genome. The EST data is available as ESTDB at http://mycompdb.bioinfo-portal.cdac.in/cgi-bin/est/index.cgi.
Conclusion
3946 unique transcripts were successfully identified from the adult female A. stephensi midgut tissue. These data can be used for microarray development for better understanding of vector-parasite relationship and to study differences or similarities with other malaria vectors. Mapping of putative novel transcripts from A. stephensi on the A. gambiae genome proved fruitful in identification and annotation of several genes. Failure of some novel transcripts to map on the A. gambiae genome indicates existence of substantial genomic dissimilarities between these two potent malaria vectors.
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