The ability of cells to undergo a regulated and programmed death is critical to the development and survival of multi-cellular organisms (Cory et al., 2003
; Youle & Strasser, 2008
). De-regulation of the processes that govern this mode of cell death, known as apoptosis, has been linked to developmental disorders and diseases, including some cancers (Horvitz, 1999
). The intrinsic apoptotic pathway is regulated, in large part, by the mitochondrial outer membrane permeabilization (MOMP); when MOMP occurs, cytochrome c is released, and downstream cysteinyl aspartate-specific proteases, called caspases become activated (Riedl & Salvesen, 2007
). Members of the BCL2 (B
ymphoma, BCL) family have the ability to regulate MOMP, positively or negatively, and are divided into two classes, pro- or anti-apoptotic, based on whether they lead to MOMP (pro-apoptotic) or inhibit MOMP (anti-apoptotic).
Since the initial discovery of BCL2, 24 additional family members have been identified based on the presence of a small amino acid motif, known as the BCL homology domain (BH) and only six members perform anti-apoptotic functions (BCL2, BCLxl/BCL2L1, BCLw/BCL2L2, BFL1/BCL2A1, MCL1, and BCLb/BCL2L10) (Lanave et al., 2004
). Although each of the six anti-apoptotic BCL family members has been shown, in vitro
, to inhibit apoptosis caused by various stimuli, there is mounting evidence that these proteins also perform distinct functions. The strongest support for this came from the analysis of mice engineered to lack the expression of individual BCL genes. The phenotypes of BCL-null mice range from embryonic lethality (BCLxl and MCL1), to abnormal death of sperm cells (BCLw), to no identifiable phenotype (BCLb) (Youle & Strasser, 2008
Multiple studies have recently examined the in vitro
protein-protein interactions and relative anti-apoptotic abilities of all six anti-apoptotic BCL family members (Chen et al., 2005
; Zhai et al., 2006
). However, a comprehensive in vivo
comparison of the oncogenic potential of the six BCL family members has not been reported. To date, only BCL2 and BCLxl have been shown to be bona fide oncogenes (Swanson et al., 2004
; Vaux et al., 1988
). However, recent findings suggest that some BCL family members (MCL1, BCLw) are over-expressed in human malignancies (Chen et al., 2007
; Kawasaki et al., 2007
; Wesarg et al., 2007
). Therefore, we decided to use a mouse model of MYC-induced leukemogenesis, in which hematopoietic progentior cells are transduced with retroviral vectors, ex vivo, followed by re-introduction into lethally irradiated sygeneic recipients. In fact, multiple groups have used variations of this, in vivo
, assay to demonstrate the cooperative oncogenic potential for numerous genes, including BCL2 and MYC (Hemann et al., 2005
; Luo et al., 2005
There are several dissimilarities among the anti-apoptotic BCL family members; for instance, the amino acid sequence identities, compared to BCL2, range from 43.8% to 22.1% (). In addition, analysis using the “SMART” algorithm indicates that, while all six proteins encode at least three BCL homology domains (BH1-3), only BCL2, BCLxl, and BCLw encode a fourth BH domain near the amino termini of the proteins (Schultz et al., 1998
). Furthermore, five of the family members encode recognizable trans-membrane domains that would potentiate insertion into the outer mitochondrial membrane, whereas BFL1 lacks this motif. Finally, a phylogeny algorithm indicated that BCL2, BCLxl and BCLw comprise a group closer in relative evolutionary time, whereas BCLb, MCL1 and BFL1 appear to comprise a distinct evolutionary group that is less related () (Larkin et al., 2007
Figure 1 BCL proteins and experimental design. (a) To show that anti-apoptotic BCL proteins encode related, but distinct proteins, sequences were entered into the “ALIGN” program and the amino acid sequence identity of each full-length protein (more ...)
Taking into consideration the levels of sequence divergence among the six BCL proteins and the observations made by others regarding sub-cellular localization and biochemical interactions, we decided to test whether each of the BCL genes could cooperate with MYC in leukemogenesis. Retroviruses used to infect bone marrow cells prepared from mice harboring a tetracycline trans-activator (tTA) dependent MYC allele (Tet-O-MYC), allowed for constitutive expression of the BCL gene from the provirus and tTA-dependent expression of the Tet-O-MYC transgene (). Following infection ex vivo, cells were transplanted into the tail vein of lethally irradiated syngeneic recipients, and disease occurrence was monitored by the presentation of clinical signs, including lethargy, ruffled fur, a distended abdomen and occasionally hind limb paralysis (). Upon presentation of disease, usually between twenty and ninety days following transplant, mice were euthanized for further analysis.
Figure 2 Induction of leukemia by cooperative action of MYC and BCL family members. (a) Kaplan-Meier survival curves are the compilation of two independent sets of bone marrow infections and transplantations performed as described in , and the number (more ...)
Mice that received cells co-expressing MYC and any of the BCL genes showed accelerated tumorigenesis compared to mice that received cells that expressed either MYC and luciferase or MYC and GFP (). Although each of the BCL genes significantly cooperated with MYC to accelerate disease onset, it appeared that the three closest related genes (BCL2, BCLxl and BCLw) were the most potent, with all mice succumbing to disease within thirty days post-transplant. Regardless of the genotype of the leukemic cells, all mice displayed organomegaly of spleen and liver, which was caused by leukemic cells disrupting the architecture of the spleen and infiltrating into the liver (). Leukemic cells initiated by either MYC alone or MYC plus BCL genes had uniform rates of proliferation, as shown by immunohistochemistry with two markers of proliferation (phospho-histone H3 or Ki67), indicating that expression of BCL proteins did not significantly increase cellular proliferation ( and data not shown). In addition, multiple tumors expressing MYC + BCL genes that were transplanted into sub-lethally irradiated secondary recipient mice readily formed tumors that exactly mimicked the primary malignancies. Taken together, these data indicate that co-expression of MYC and any of the individual BCL family members increases the penetrance and decreases the latency of tumorigenesis, without significantly altering the proliferation rates, compared to expression of MYC alone.
Although all BCL family members were capable of cooperating with MYC in leukemogenesis, it was important to determine whether the leukemic cell phenotype was altered when different BCL family members were expressed. To address this issue, we examined cell surface markers, known to be expressed on cells of either the lymphoid or myeloid lineages, from various hematopoietic tissues- spleen, thymus, lymph nodes and marrow of the long bones- by flow cytometry. Irrespective of whether tumors were initiated by MYC alone or by MYC and BCL genes, we observed a profound increase in the number of cells that express the myeloid markers Gr-1 and Mac-1(CD11b) in the spleen and bone marrow (). However, lymphopoiesis was not overtly perturbed in the leukemic mice, as the thymus and lymph nodes contained normal ratios of resident cells and there were no increases in the numbers of lymphoid cells in the spleen (not shown and Supplemental 1
). Since tumor cells reside primarily in the bone and spleen and express the Gr-1 and Mac-1 cell surface markers, the malignancies resemble acute myeloid leukemia (AML).
Figure 3 MYC-induced leukemias resemble acute myelogenous leukemia regardless of cooperating BCL gene. (a) Cells from all mice that required euthanization were analyzed by flow cytometry to determine the phenotypic nature of the disease. All mice examined had (more ...)
Next, we measured levels of MYC and BCL proteins in whole spleen samples by western blotting. Endogenous Myc was detected in cells of the normal spleen, with consistently increased levels of MYC protein observed in each of the tumor samples, consistent with induction of the transgene in the tumor cells (). In contrast, specific antibodies for each of the BCL proteins clearly show high levels of the respective BCL protein in the tumor samples. Only endogenous BFL1 was detected in whole spleen extracts from non-leukemic mice, consistent with previous data demonstrating expression of BFL1 in the hematopoietic compartment (Mandal et al., 2005
To explore whether our observations using a MYC-driven mouse model of AML might be relevant to human malignancies, we measured expression of each of the BCL genes in a panel of solid human tumors and leukemia/lymphoma cell lines. Using the “TissueScan oncology survey panel” from Origene, we performed quantitative real time PCR analysis (qPCR) on cDNA acquired from 60 different tissues and tumor samples for all six BCL genes, plus ACTIN as a control (). The panel consists of cDNA derived from five tissues- 3 “normal” and 9 tumor samples for each tissue (breast, liver, lung, ovarian and prostrate)- deposited into individual wells. Following qPCR we observed elevated levels of BFL1 in eight of nine breast tumor samples (dark bars) and three of nine colon tumor samples, compared to the normal tissue controls (light bars). Increased BCL2 expression was also detected in seven of the nine breast cancer samples, and BCLxl was over-expressed in a number of ovarian tumors. BCLw was the only additional member that was highly expressed in more than two tumor samples from a particular tissue, being elevated in three lung cancer patients.
Figure 4 BCL family members are expressed in human solid tumor samples and in leukemia/lymphoma cell lines. (a) Expression of BCL genes in human solid tumor samples. TissueScan oncology survey cDNA panels were obtained from OriGene (Rockville, MD, USA) and quantitative (more ...)
As further support for the possible implications of our in vivo mouse studies, we sought to determine if BCL members and MYC are co-expressed in leukemia and lymphoma cell lines derived from human patients. All six BCL members were readily detectable in various human leukemia/lymphoma cell lines by western blotting with antibodies specific for each BCL protein. Importantly, MYC protein was also detected in each sample, indicating that the experiments conducted in this study, using MYC-induced murine AML, are likely pertinent to the study of human malignancies.
If BCL family members play a causal role in human neoplasms, it is important to determine whether inhibiting their anti-apoptotic effects will have therapeutic benefits. It has been known for more than ten years that over-expression of only the BH3 domain of certain pro-apoptotic BCL family members is sufficient to block the activity of BCLxl and BCL2 and can cause apoptosis in cultured tumor cells (Cosulich et al., 1997
; Holinger et al., 1999
). In addition, deinduction of an inducible BCL2 transgene, in the presence of a constitutive MYC allele, was sufficient to cause regression of lymphoid tumors in mice (Letai et al., 2004
). This suggested that inhibiting the function of an anti-apoptotic gene (BCL2), even in the context of additional oncogenic lesions (MYC), might be sufficient to extend lives of patients with lymphoma over-expressing BCL2.
Such studies have stimulated research designed to target the anti-apoptotic machinery, and several small molecules that inhibit the anti-apoptotic BCL family members by binding within the BH3 hydrophobic pocket have been identified. One of the most promising first generation molecules, ABT-737, has a very high specificity for inhibiting BCL2, BCLxl and BCLw and has shown promise against many tumor types, including leukemias and solid tumors, as a single agent and in combination with other therapies (Kohl et al., 2007
; Konopleva et al., 2006
; Reed, 2006
; Shoemaker et al., 2008
). For instance, 8 out of 9 primary CD34+ human AML samples efficiently underwent apoptosis in the presence of ABT-737, whereas 3 out of 4 normal CD34+ stem cell samples were unaffected by the drug (Konopleva et al., 2006
). Although the bulk of the work with this compound has been in cell culture and in animal models, this molecule, along with several others, is currently being tested in clinical trials to determine safety and efficacy in patients.
BCL genes may also have a role in both primary and acquired drug resistance. For example, increased expression of BCL2, BCLxl and BCLb has been observed in AML cell lines resistant to high levels of busulfan treatment, and increased copy numbers of genomic loci encoding BCLw, MCL1 and BCLb were identified in various cell lines that were treated with, and became resistant to, etoposide, campothecin or Ara-C, respectively (Valdez et al., 2008
; Yasui et al., 2004
). These data add emphasis to the findings we report here and support the need to understand the role of all BCL genes in cancer and to consider targeting multiple members of the BCL family in both primary and drug-resistant tumors.