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We conducted the first phase 0 clinical trial in oncology of a therapeutic agent under the Exploratory Investigational New Drug Guidance of the US Food and Drug Administration. It was a first-in-human study of the poly (ADP-ribose) polymerase (PARP) inhibitor ABT-888 in patients with advanced malignancies.
ABT-888 was administered as a single oral dose of 10, 25, or 50 mg to determine the dose range and time course over which ABT-888 inhibits PARP activity in tumor samples and peripheral blood mononuclear cells, and to evaluate ABT-888 pharmacokinetics. Blood samples and tumor biopsies were obtained pre- and postdrug administration for evaluation of PARP activity and pharmacokinetics. A novel statistical approach was developed and utilized to study pharmacodynamic modulation as the primary end point for trials of limited sample size.
Thirteen patients with advanced malignancies received the study drug; nine patients underwent paired tumor biopsies. ABT-888 demonstrated good oral bioavailability and was well tolerated. Statistically significant inhibition of poly (ADP-ribose) levels was observed in tumor biopsies and peripheral blood mononuclear cells at the 25-mg and 50-mg dose levels.
Within 5 months of study activation, we obtained pivotal biochemical and pharmacokinetic data that have guided the design of subsequent phase I trials of ABT-888 in combination with DNA-damaging agents. In addition to accelerating the development of ABT-888, the rapid conclusion of this trial demonstrates the feasibility of conducting proof-of-principle phase 0 trials as part of an alternative paradigm for early drug development in oncology.
The low success rate of new molecular entities and the development of molecularly targeted agents for the treatment of cancer have necessitated re-evaluation of the standard anticancer drug development paradigm.1 Recognizing that lack of predictive preclinical models, prolonged timelines, and high costs have hampered drug discovery, the US Food and Drug Administration developed the Exploratory Investigational New Drug (IND) Guidance to provide new regulatory pathways to enhance the drug development process.2 Because phase 0 trials conducted under an exploratory IND involve nontoxic drug doses administered for short periods to limited numbers of patients, the preclinical toxicology data required to support the IND are less extensive; thus, these first-in-human trials, although lacking therapeutic intent, can be initiated earlier than traditional phase I studies. By providing essential pharmacodynamic (PD) and pharmacokinetic (PK) data at the initial stage of the clinical trials process, phase 0 studies can inform and expedite the subsequent development of a promising agent.3 However, this requires validated assays and standardized tissue handling procedures for consistent results. We hypothesized that a potent, molecularly targeted modulator of chemotherapeutic efficacy would be an ideal candidate to test whether early evidence of target modulation might speed drug development.
Poly (ADP-ribose) polymerase (PARP)-1 and PARP-2 are involved in DNA repair via poly (ADP-ribosyl)ation of histones and DNA repair enzymes; elevated PARP levels can result in resistance to cytotoxic chemotherapy and radiation.4–10 Thus, PARP inhibitors hold promise as chemotherapy and radiation sensitizers.11–14 ABT-888, an orally bioavailable inhibitor of PARP, was studied because it possessed a wide margin of safety relative to its target-modulating dose in preclinical models, and demonstration of target modulation in human samples was critical to its subsequent development.15–18
This is the first report of a phase 0 clinical trial of a therapeutic agent in oncology with target modulation as the primary end point conducted under the Exploratory IND Guidance. Trial objectives were to determine a dose range and time course over which ABT-888 inhibited PARP activity (measured using a validated PD assay for PAR (poly [ADP-ribose], a product of PARP) in tumor and peripheral blood mononuclear cells (PBMCs), as well as the PK of ABT-888. We also developed and utilized a novel statistical approach to study PD modulation as a primary end point for trials of limited sample size.
Adult patients with advanced malignancies refractory to at least one line of standard treatment were eligible, as were patients with chronic lymphocytic leukemia and follicular lymphoma if they had disease for which standard therapy was currently not indicated. Patients with primary brain tumors, brain metastases, or a history of seizures were excluded because high-dose ABT-888 caused seizures in a preclinical animal model. Prior antineoplastic therapy must have been completed at least 2 weeks before enrollment.
The objectives and the nontherapeutic nature of the trial were discussed in detail with potential patients, who were given ample opportunity to review and discuss the consent document with study investigators, family members, and referring physicians. Before signing the consent form, patients were asked to verbalize their understanding of the nature of the trial, and the need for tumor biopsies. This trial was conducted under a National Cancer Institute (NCI)–sponsored exploratory IND with approval from the National Institutes of Health Institutional Ethics Committee and the NCI institutional review board. Protocol design and conduct followed all applicable regulations, guidances, and local policies.
ABT-888 was supplied by the NCI Division of Cancer Treatment and Diagnosis under a collaborative agreement with Abbott Laboratories. A single oral dose of ABT-888 was administered on day 1, with serial blood sampling for PD and PK analyses performed before and after drug administration (Fig 1). Significant toxicities were defined as those considered related to ABT-888 administration and were grade ≥ 2 nonhematologic events or thrombocytopenia, and grade ≥ 3 anemia, leucopenia, or neutropenia, reported using the NCI Common Toxicity Criteria for Adverse Events version 3.0. If one patient developed significant toxicity, no additional patients could be enrolled, and the study would be put on hold.
Five dose levels, each with three patients, were planned: 10, 25, 50, 100, and 150 mg. The 10-mg starting dose was based on 1/50th of the no observed adverse effect level from a 2-week study in the most sensitive species (dog), as recommended in the Exploratory IND Guidance.2 The objective of dose escalation was to achieve statistically and biologically significant inhibition of PAR levels at nontoxic dose levels, not to determine a maximum tolerated dose (MTD).
To minimize the possibility of performing tumor biopsies in patients receiving doses unlikely to show drug effects, biopsies were obtained once significant inhibition of PARP activity (ie, 50% reduction in PAR levels) was observed in PBMCs from at least one of the three patients at a given dose level, or a plasma Cmax of 0.21 μmol/L (concentration associated with a significant reduction in tumor PAR levels in single-dose studies in mice) was achieved in at least one patient. All subsequent patients were then to undergo paired pre- and postdrug administration tumor biopsies (Fig 1). To proceed with sampling for PD analyses after drug administration, patients were required to have a minimum baseline PAR level (31 pg PAR per mL per 2.5 × 105 cells) to allow demonstration of a 50% reduction in PARP activity. All patients underwent blood and urine sampling for PK analyses.
Blood samples for PK analysis were at multiple time points before and within 24 hours after drug administration (Fig 1; online only Appendix). A high performance liquid chromatography-based assay with ultraviolet and mass spectrometric detection was used to measure levels of parent drug for PK analyses.19
Baseline and post-ABT-888 administration PAR levels were measured in PBMCs as indicated (Fig 1). Percutaneous tumor biopsies were obtained using either an 18-gauge needle under radiologic guidance (five patients) or a dermal biopsy punch (four patients; Appendix).
The PAR assay is an immunoassay with purified monoclonal antibody to PAR as the capture reagent and rabbit anti-PAR antiserum (#4336-BPC-100; Trevigen Inc, Gaithersburg, MD) as the detecting agent. Antirabbit horseradish peroxidase conjugate (#074-15-061; Kirkegaard & Perry Laboratories Inc, Gaithersburg, MD) is the chemiluminescence reporter. Assay analytic performance met validation criteria.18
The trial employed a novel statistical evaluation scheme developed specifically for phase 0 trials, where the end points are PD measurements rather than toxicity.20 Significant inhibition of PARP activity was defined as a reduction in PAR levels 3 to 6 hours after administration of ABT-888, compared to baseline, that satisfied two criteria: reduction was at least 50%, and reduction was sufficient, when compared to the variation among the baseline values, to yield 90% statistical confidence that it was not due to chance variation. Significant inhibition of PARP activity for a dose level was declared if two of three patients had significant inhibition in either PBMCs or tumor. For either end point, at each dose level there is 90% power to detect a true 80% rate of significant inhibition across patients, with a false-positive rate of .03. For PBMCs, the PAR level reduction threshold and the pooled standard deviations (SD) were based on intrapatient preadministration variability (four baseline measurements per patient), and for tumor on the interpatient preadministration variability (single baseline measurement per patient). Variability was measured on log-transformed values. For both tumor and PBMC measures, the difference between pre- and post-treatment log PAR values was compared to the threshold of 1.8 SD (from the corresponding pretreatment measures) to establish statistically significant reduction at the one-sided .10 level. It was recognized that interpatient variability being greater than intrapatient variability could make demonstration of statistically significant inhibition in tumor difficult.
Patient demographics are presented in Table 1. Nine paired tumor biopsies were performed, all without complications.
ABT-888 was well tolerated; no significant adverse effects were observed. One patient at the 10-mg dose level had an episode of mild dizziness and nausea relieved with food after receiving ABT-888; this patient had a history of recurrent nausea and dizziness associated with taking narcotics and had received his regular dose of narcotic around the time of ABT-888 administration. One patient at the 25-mg dose level developed mild dysgeusia for 3 days post-drug administration that was not associated with anorexia or decreased oral intake.
ABT-888 was rapidly absorbed, and peak plasma levels occurred between 0.5 and 1.5 hours after dosing (Fig 2; Appendix Table A1). The target Cmax of 0.21 μmol/L was exceeded in the first patient cohort; thus, all subsequent patients agreed to undergo paired pre- and post-treatment tumor biopsies. Clearance of ABT-888 in urine was rapid, and at the 50-mg dose level a large quantity of unchanged parent drug was recovered in the urine (average 70% in 24 hours; range 31% to 115%).
Baseline PAR levels in 11 of 13 patient PBMC samples and nine of 10 patient tumor samples were above the defined minimum to allow further sampling after drug administration. Post-drug PBMC PAR levels were compared to the day 1 sample (baseline) level collected immediately before ABT-888 administration (Figs 3A, A,3B).3B). The threshold for declaring statistically significant inhibition was calculated to be a 55% reduction in PAR in PBMCs and 95% in tumors.
Statistically significant reductions in PAR levels were observed in tumor samples from two of the three patients at the 25-mg dose (the third was near borderline with reduction corresponding to P = .14; one sided), and in PBMCs from both the assessable patients (Fig 3A). At the 50-mg dose, statistically significant reductions in PAR levels in tumor were observed in two of three assessable patients and in PBMCs from 4 of 6 evaluable patients. Therefore, by our statistical criteria using the binomial distribution, statistically significant reduction in PAR levels was observed for both tumor and PBMC samples at the 25-mg and 50-mg doses. By the binomial distribution, given a false-positive probability of .10 for observing significant PAR level reduction for an individual patient under the null hypothesis of no effect at that dose level, the P value associated with observing significant reduction in two of three patients is P = .03, in two of two patients is P = .01, and in four of six patients is P = .001.
Patient 11 received 50 mg of ABT-888 but had no significant reduction in PAR levels in either PBMCs or tumor (Figs 3A, A,3B).3B). PK analysis revealed plasma levels comparable to the other patients in the 50-mg cohort. Ex vivo treatment of a PBMC sample from this patient with 0.21 μmol/L (target Cmax), or with 0.8 μmol/L ABT-888 (the patient's actual Cmax), for 2 hours had no detectable effect on PAR levels. In comparison, PAR reduction in ex vivo PBMC samples from four healthy volunteers and from another patient at the same dose level evaluated in the same experiment was more than 90%, consistent with results from our previous studies on ex vivo PBMC sensitivity to ABT-888.21 Comparison of this patient's samples with another study patient and three healthy donors did not identify unique single nucleotide polymorphisms (SNPs)22 or significant differences in the ratio of PARP to poly (ADP-ribose) glycohydrolase (PARG), as measured by real-time quantitative polymerase chain reaction, that would explain these results (Appendix).
Three additional patients at the 50-mg dose level underwent a tumor biopsy at 24 ± 3 hours after ABT-888 administration to evaluate the time to recovery of PARP activity (Fig 3C). PAR levels were at least 49% below baseline levels 24 hours after drug administration, but this reduction was significant in only one of the patients.
We calculated the Pearson correlation coefficient between log10 reduction measures for the eight participants with PAR reduction in both PBMCs and tumor samples. The estimated correlation was relatively modest (r = 0.51) and did not achieve statistical significance (P = .12, one sided) against the null hypothesis of r = 0. The estimated linear regression line for log10 tumor PAR level reduction versus log10 PBMC PAR level reduction did have a slope of 1 and a constant of 0.75, indicating that PAR level reduction in PBMCs, on average, tracks PAR level reduction in tumor, but is approximately six-fold less.
For patients whose tumor biopsy PAR levels were reduced after ABT-888 administration, the Pearson correlation of PAR level reduction in PBMCs (at 4 hours) versus the ABT-888 area under the curve (AUC) was statistically significant (r = 0.56; P = .05, one sided) versus r = 0, but depended on the positive dose-response relationships for the two variables. It disappeared when we stratified for dose (stratified r = −0.02, where we correlated individual dose means rather than overall means). Tumor PAR level reduction at 3 to 6 hours did not significantly correlate with the ABT-888 AUC. Similar results were obtained when Cmax measures were substituted for AUC measures.
In this study, we present the results of the first phase 0 clinical trial of a therapeutic agent in oncology with a PD primary end point. The trial is significant in that it provided in a short period of time both the molecular proof-of-target inhibition by ABT-888 in tumor, as well as the PK and PD data that served as the foundation for subsequent combination studies of ABT-888 with DNA-damaging agents. As shown in Figure 4, these data were available within 5 months of starting the phase 0 trial. Starting a standard phase I investigation of drug combinations based on PK and PD results from a phase 0 trial without first determining the MTD of ABT-888 as a single agent is well suited to the evaluation of molecularly-targeted agents in combination with other targeted agents or cytotoxics.
Greater than 90% inhibition of PAR levels was observed 3 to 6 hours post-drug administration, with recovery at 24 hours in both xenograft models18 and the clinical trial. This supports a twice daily schedule for ABT-888 administration in subsequent trials, ensuring adequate inhibition of PAR and optimizing the likelihood of clinical benefit. Based on the significant inhibition of PAR in tumor biopsies at 25 mg and the available capsule strengths, the recommended phase I dose of ABT-888 in combination with DNA-damaging agents is 10 mg twice a day.
Phase 0 studies with PD modulation as the primary end point rely on the PD assay for making decisions including dose escalation or defining effective target modulation. Therefore, the analytic performance of an assay is critical, and the assay needs to be validated before trial initiation.18,23 The PD assay, timing of sample collection, and sample storage and handling procedures used in this trial were all based on extensive preclinical investigations specifically designed to validate assay techniques and to establish standard operating procedures.3,18 It is important to emphasize that the rapid completion of complex, early-phase clinical trials requires an integrated, multidisciplinary research team capable of performing PK and PD studies in real time (in this trial, 48 hours or less after sample acquisition).
We observed one patient in our 50-mg dose cohort whose ABT-888 plasma Cmax and AUC levels approximated the mean for all other patients at that dose level, but who demonstrated no decrease in PAR levels in PBMCs or tumor after drug exposure. We investigated whether a mutation in the PARP gene or significantly low levels of PARG could account for the lack of observed drug effect. No unique SNPs were identified, and the PARP/PARG ratio was not significantly different when compared to other patients or healthy donors. The mechanism of resistance to PARP inhibitor therapy needs to be further explored. However, our ability to accurately confirm the lack of responsiveness by treating PBMCs ex vivo with ABT-888 raises the possibility of ex vivo screening of PBMCs from patients in future trials to detect those likely to respond to ABT-888.
Tumor biopsies for research purposes are often obtained in cancer clinical trials; however, it has been argued that the perception of benefit could be influencing the acceptance of invasive procedures in such studies.24,25 However, the consent to obtain tumor biopsies in this phase 0 trial was given with the clear understanding of the nontherapeutic nature of such a procedure.26
There are very few publications demonstrating the value of results from research biopsies obtained during early-phase clinical trials.25 We suggest that it is not appropriate to ask patients for biopsy samples unless the assay procedures to be employed have been carefully validated. As demonstrated in this study, preclinical assay qualification can permit scientifically meaningful and statistically valid conclusions to be drawn from a limited number of biopsy samples. We understand that phase 0 studies may be more difficult to perform outside of the NCI because of the need for both a highly motivated patient population and substantive research resources to develop and validate the assays and obtain multiple tumor biopsies.
Using the novel statistical evaluation scheme that we developed specifically for use in phase 0 trials, we demonstrated statistically significant inhibition of PAR levels in both tumor and PBMCs after a single dose of ABT-888. The statistical correlation observed between the effects of ABT-888 in PBMCs versus tumor samples raises the possibility of using PBMCs as tumor surrogates, obviating the need for biopsies. We are evaluating this observation further in phase I ABT-888 combination trials.
The successful and expeditious conduct of this trial, and the impact it has had on the development timeline of ABT-888 (Fig 4), provide an initial example of a new paradigm for early therapeutics development in oncology. Clearly, several additional phase 0 trials will need to be completed under the Exploratory IND Guidance, and their long-term impact on improving the success rate and timeline assessed, before phase 0 trials will be considered to have an established role in the anticancer drug development process. The US Food and Drug Administration's new regulatory policy has provided an important and timely opportunity to expeditiously conduct and complete novel, proof-of-principle clinical trials of molecularly targeted therapeutic and imaging agents. The potential for a major impact of phase 0 trials and the exploratory IND on developing new anticancer drugs provides a strong stimulus for the broader uptake and enhanced application of carefully conceived, pharmacodynamically driven early-phase clinical trials in oncology.
We acknowledge the efforts of the following members of the phase 0 team: Yvonne Horneffer, Lamin Juwara, and Mary Ann Yancey for clinical care; Anthony Kam and Richard Chang, Interventional Radiology; Yiping Zhang, Kate Luyegu, Sylvan McDowell, and Dwight Simmons for specimen handling and processing; Weimin Zhu for polymerase chain reaction data and Sonny Khin for immunoassay data; Eva Majerova and Kimberly Hill for pharmacokinetic analysis; and Gina Uhlenbrauck for editorial assistance in the preparation of this manuscript.
Blood samples were collected in 7-mL EDTA tubes at baseline and 0.5, 1, 1.5, 2, 3, 4, 7, 12, and 24 hours after ABT-888 administration. Within 15 minutes of collection, blood samples were centrifuged, and plasma transferred into Sarstedt tubes (Sarstedt, Leics, Newton, NC) and frozen at −80°C until testing. Urine was collected in periods of 8 hours for a total of 24 hours post-drug administration. At the end of each 8-hour period, urine volume was recorded, and 10 mL of urine sample was aliquoted and frozen at −80°C until further analysis. Standard curves were constructed by plotting the peak area ratios against the added analyte concentration in the plasma standards. Linear least squares regression was performed using a weighting factor of 1/yobserved without inclusion of the origin, to determine the slope, y-intercept, and correlation coefficient of the best fit line. Analyte concentrations in unknown samples were calculated using results of the regression analysis. Each unknown sample was initially assayed in duplicate with additional analyses performed if the replicate determinations deviated by more than 10%. Specimens with concentrations exceeding the upper limit of the standard curve were assayed on appropriate dilution with blank plasma.
Accuracy and precision of the pharmacokinetic (PK) assay were determined by analyzing the back-calculated sample concentrations and regression parameters from a series of standard curves of ABT-888 in plasma that were prepared and analyzed on separate days. The relative standard deviation (RSD) of the mean-predicted concentrations for the independently assayed standards provided the measure of precision. The lower limit of quantitation was defined as the minimum concentration amenable to analysis with an inter-day RSD not exceeding 20%. Accuracy of the assay was assessed by expressing the mean-predicted analyte concentration as a percentage of its known concentration in the standard solutions.
Blood was collected in Cell Prep Tubes (Becton Dickinson, Franklin Lakes, NJ) and peripheral blood mononuclear cells (PBMCs) isolated within 1 hour, washed in Plasma-Lyte A USP (Baxter, Deerfield, IL) and pelleted by low-speed centrifugation. After resuspension in Plasma-Lyte A, viable cells were counted using a hemacytometer with trypan blue and adjusted to a concentration of 107 viable PBMCs/mL. Aliquots were transferred into a conical cryovial and PBMCs recovered by centrifugation as cell pellets, which were flash frozen and stored at −80°C until use in the immunoassay.
Frozen cell pellets were suspended in lysis buffer (Biosource, Camarillo, CA), supplemented with protease inhibitor cocktail tablets (Roche Applied Science, Indianapolis, IN) and 1% phenylmethanesulfonyl fluoride (Sigma-Aldrich, St Louis, MO) and lysed by sonication followed by vortexing. Sodium dodecyl sulfate (SDS) was added to each aliquot from a 20% stock (Ambion Inc, Austin, TX) to 1% SDS, followed by boiling for 5 minutes to inactivate enzymes. Aliquots were then snap-cooled in an ice bath until they reached ambient temperature. PBMC lysates were assayed immediately, using 25 μL of extract (the equivalent of 250,000 PBMCs) per well in the poly (ADP-ribose) (PAR) immunoassay.
Biopsy specimens were immediately placed onto sterile glass slides and within 1 minute transferred into prechilled 1.5-mL cryogenic vials, then stored in liquid nitrogen. While kept on an ice bath, frozen needle biopsy specimens were thawed and extracted by adding 0.5 mL lysis buffer (same as for PBMCs), minced with fine-point scissors, vortexed, and sonicated with additional vortexing. After standing in an ice bath 15 minutes and vortexing again, SDS was added to each aliquot from a 20% stock to 1% SDS, and the tubes vortexed and then boiled in a water bath for 5 minutes. Subsequently, the tubes were snap-cooled for 1 minute in an ice bath, moved to ambient temperature, and after vortexing, clarified by centrifugation at 10,000 × g for 2 minutes at 4°C.
Single-strand cDNA was prepared using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA); 12 forward and reverse primer sets with M13 tails (EasyExonPrimer: http://126.96.36.199/%7Eprimer/) were used to amplify 500- to 700-bp overlapping fragments of cDNA representing the PARP-1 gene (NM_001618). Sequencing reactions were performed with a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) then run on a 3730xl DNA Analyzer (Applied Biosystems). Base calling, quality assessment, and assembly were carried out using the Phred-Phrap software suite (http://www.phrap.com/index.htm), and single nucleotide polymorphisms (SNP) calling by Mutation Surveyor (Soft Genetics, State College, PA), with sequence variants verified by manual inspection of the chromatograms. The sample from patient 11 was sequenced 5 times using two separate cDNA preparations, while each of the three volunteer samples and the ABT-888-responsive patient sample were sequenced in triplicate.
PBMCs were processed and flash frozen as for the PAR immunoassay. Cell pellets were extracted in RNAqueous (Ambion Inc). The resulting nucleic acid extract was then processed through a Promega (Madison, WI) RQ1 DNAse treatment step, and the product quantified on a Nanodrop Spectrophotometer. Primer sets for RTQ-PCR were: actin (#PPH00073A-200; SA Biosciences, Frederick, MD); PARP-1 (F-5′-tac cac ttc tcc tgc ttc tgg a-3′, R-5′-agc ttc cgc tgt ctt ctt ga-3′); PARP-2 (F-5′-act ctt ccc ctg cca aga aa-3′, R-5′-ttc ctg gca tac cat ctt gc-3′); and PARG (F-5′-tgc cag aga gtc cat tgt ca-3′, R-5′-cgg gtt cac ttt cct tct ca-3′). PARP and PARG primer sets were validated by Applied Biosystems, and were synthesized by The Midland Certified Reagents Company (Midland, TX).
The real-time quantitative polymerase chain reaction human reference cDNA was from Clontech (#639654; Mountain View, CA). Amplification was performed on Taqman 7500 instruments using SYBR Green PCR Master Mix (#4364346; Applied Biosystems). Amplification was performed with 2 μg cDNA for actin and PARP1, and 5 μg cDNA for PARP-2 and PARG. RNA isolation from PBMCs was performed using Ambion's RNAqueous phenol-free RNA isolation kit (#1912). DNA-free RNA was generated using Promega's RQ1 RNase-free DNase (#M6101).
First-strand cDNA synthesis was performed with Invitrogen's SuperScript III First-Strand Synthesis System for RT-PCR kit (#18080-051, Carlsbad, CA,) using the random hexamer primers supplied with the kit. All kits were used according to manufacturer's instructions. The PARP-1/PARG ratio was calculated using actin-normalized PARP and PARG values.
One of the possible explanations for the lack of sensitivity of patient 11 to ABT-888 could be low levels of the enzyme PARG, which is responsible for degrading the PAR polymer. Even if PARP activity was inhibited by ABT-888, the absence of functional PARG would lead to the inaccurate conclusion that PARP had not been inhibited by ABT-888. Real-time quantitative polymerase chain reaction analysis did not reveal a significant difference in the ratio of PARP to PARG for patient 11 compared with another patient who demonstrated drug effect as well as three healthy donors. Multiple SNPs have been reported for the PARP gene (National Center for Biotechnology Information Entrez SNP Database: http://www.ncbi.nlm.nih.gov/sites/entrez?db = snp). To evaluate the possibility that a pharmacogenomic effect explained our results, we sequenced the cDNA from PBMCs of patient 11 and of another patient who showed PAR inhibition after ABT-888 administration, as well as normal donors. No unique modifications were identified, indicating that the lack of responsiveness to ABT-888 was unlikely to be the result of a mutation in the coding sequence of the PARP gene. In five replicate sequence analyses, the same synonymous SNP (rs1805415) in the automodification/protein-protein interaction region of the gene (frequency 0.37) was identified in patient 11 and all other samples examined.
|Collection Time (hour)||Plasma ABT-888 (μM) by Patient No.|
|Cohort 1 (10 mg)||Cohort 2 (25 mg)||Cohort 3 (50 mg)|
|AUC (last), hour × μM||0.78||0.98||1.15||3.01||2.61||2.7||5.44||3.64||6.64||6.96||8.55||5.38||5.47|
NOTE. Blood samples were collected from patients in cohorts 1, 2, and 3 before and after administration of a single 10-, 25-, or 50-mg dose of ABT-888 for analyses. All unit values are micromolar. Collection times (baseline to 24 hours after ABT-888 administration) are indicated in the left-hand column. Average AUC (last) values for each cohort are indicated with standard deviations.
Abbreviations: LLQ, lower limit of quantification; LOD, lower limit of detection; AUC, area under the curve.
Supported in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. This research was also supported by the Division of Cancer Treatment and Diagnosis and the Center for Cancer Research of the National Cancer Institute.
Presented in part at the 43rd Annual Meeting of the American Society of Clinical Oncology, Chicago, IL, June 1-5, 2007.
Terms in blue are defined in the glossary, found at the end of this article and online at www.jco.org.
Authors' disclosures of potential conflicts of interest and author contributions are found at the end of this article.
Clinical Trials repository link available on JCO.org.
Clinical trial information can be found for the following: NCT00387608.
Although all authors completed the disclosure declaration, the following author(s) indicated a financial or other interest that is relevant to the subject matter under consideration in this article. Certain relationships marked with a “U” are those for which no compensation was received; those relationships marked with a “C” were compensated. For a detailed description of the disclosure categories, or for more information about ASCO's conflict of interest policy, please refer to the Author Disclosure Declaration and the Disclosures of Potential Conflicts of Interest section in Information for Contributors.
Employment or Leadership Position: Jennifer A. Low, Genentech Inc (C); Helen Eliopoulos, Abbott Laboratories (C); Vincent L. Giranda, Abbott Laboratories (C); Gary Gordon, Abbott Laboratories (C) Consultant or Advisory Role: Robert Kinders, Trevigen Inc (C), Kirkegaard & Perry Laboratories Inc (U) Stock Ownership: Jennifer A. Low, Genentech Inc; Helen Eliopoulos, Abbott Laboratories; Vincent L. Giranda, Abbott Laboratories; Gary Gordon, Abbott Laboratories Honoraria: None Research Funding: None Expert Testimony: None Other Remuneration: None
Conception and design: Shivaani Kummar, Robert Kinders, Martin E. Gutierrez, Larry Rubinstein, Ralph E. Parchment, Jennifer A. Low, Alice Chen, Anthony J. Murgo, Jerry Collins, Seth M. Steinberg, Helen Eliopoulos, Vincent L. Giranda, Gary Gordon, Robert Wiltrout, Joseph E. Tomaszewski, James H. Doroshow
Financial support: Robert Wiltrout, James H. Doroshow
Administrative support: Ralph E. Parchment, Jiuping Ji, Lee Helman, Joseph E. Tomaszewski, James H. Doroshow
Provision of study materials or patients: Shivaani Kummar, Martin E. Gutierrez, Ralph E. Parchment, Anthony J. Murgo, Joseph E. Tomaszewski, James H. Doroshow
Collection and assembly of data: Shivaani Kummar, Robert Kinders, Ralph E. Parchment, Lawrence R. Phillips, Jiuping Ji, Anne Monks, Jennifer A. Low, Anthony J. Murgo, Joseph E. Tomaszewski, James H. Doroshow
Data analysis and interpretation: Shivaani Kummar, Robert Kinders, Larry Rubinstein, Ralph E. Parchment, Lawrence R. Phillips, Jiuping Ji, Anne Monks, Jennifer A. Low, Alice Chen, Anthony J. Murgo, Jerry Collins, Seth M. Steinberg, Joseph E. Tomaszewski, James H. Doroshow
Manuscript writing: Shivaani Kummar, Larry Rubinstein, Ralph E. Parchment, Anthony J. Murgo, Jerry Collins, Seth M. Steinberg, Robert Wiltrout, James H. Doroshow
Final approval of manuscript: Shivaani Kummar, Larry Rubinstein, Alice Chen, Anthony J. Murgo, Jerry Collins, Helen Eliopoulos, Vincent L. Giranda, Gary Gordon, James H. Doroshow