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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
From:
Traffic. Author manuscript; available in PMC 2010 April 1.
Published in final edited form as:
Traffic. 2009 April; 10(4): 372–378.
doi: 10.1111/j.1600-0854.2009.00877.x

Figure 2

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Phagosomal H2HFF-OxyBURST oxidation profiles

H2HFF-OxyBURST oxidation kinetics were plotted as calibrated fluorescence using the equation CF = OB/AF. Measurements were taken every 2 seconds for 60 minutes. Activation was accomplished by LPS (10ng/ml) addition 15 hr prior to experimentation; the trace represents the averaged values over three repetitions, error bars represent SD. B. Beads were coated in 1.0 mg or 0.5 mg H2HFF-OxyBURST and evaluated in LPS activated macrophage phagosomes. Maximal oxidation is plotted as calibrated fluorescence using the equation CF = OB/AF. Activation was accomplished by pretreatment with LPS (10 ng/ml) 15 hr prior to phagocytosis. The trace represents the averaged values over two repetitions, error bars represent SD.

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