|Home | About | Journals | Submit | Contact Us | Français|
Three N-linked glycosylation sites were removed from the envelope glycoproteins of Friend, Moloney, and AKV mouse ecotropic gammaretroviruses: gs1 and gs2, in the receptor binding domain; and gs8, in a region implicated in post-binding cell fusion. Mutants were tested for their ability to infect rodent cells expressing 4 CAT-1 receptor variants. Three mutants (Mo-gs1, Mo-gs2, Fr-gs1) infect NIH 3T3 and rat XC cells, but are severely restricted in M. dunni cells and Lec8, a Chinese hamster cell line susceptible to ecotropic virus. This restriction is reproduced in ferret cells expressing M. dunni dCAT-1, but not in cells expressing NIH 3T3 mCAT-1. Virus binding assays, pseudotype assays, and use of glycosylation inhibitors further suggest that restriction is primarily due to receptor polymorphism and, in M. dunni cells, to glycosylation of cellular proteins. Virus envelope glycan size or type does not affect infectivity. Thus, host range variation due to N-glycan deletion is receptor variant-specific, cell specific, virus type-specific, and glycan site-specific.
The initial event in the process of gammaretrovirus entry is the interaction between the viral envelope (Env) glycoprotein and its cellular receptor. For the mouse leukemia virus (MLV) gammaretroviruses, there are multiple host range variants that are determined by sequence variation in the receptor binding domain (RBD) of the Env SU (surface) component. Each host range group utilizes a different receptor, all of which are transporter proteins with multiple transmembrane domains (Tailor et al., 2003; Stocking and Kozak, 2008). Receptor binding of the RBD triggers a conformational change in Env that results in exposure of the fusion domain of the Env TM (transmembrane) component that then initiates the fusion of viral and cellular membranes.
The viral Envs as well as their cell surface receptors are modified post-translationally by glycosylation. These glycoproteins acquire N-linked oligosaccharides that are initially transferred to nascent polypeptide chains in the ER from a dolichol precursor. These glycans are subsequently modified by glycosidases and glycosyl transferases as the proteins are transported through the Golgi.
The CAT-1 receptor for the ecotropic host range subgroup of MLVs (E-MLVs) carries two consensus recognition sites for N-linked glycosylation (N-X-S/T, where X is any amino acid except P). These glycosylation sites are both occupied by N-glycans (Kim et al., 1993). Both sites are found in the 3rd extracellular loop which also carries the amino acid residues critical for receptor function (Albritton et al., 1993; Yoshimoto et al., 1993). CAT-1 receptors from which N-glycans have been removed by mutagenesis continue to mediate virus entry, and there is some indication that deglycosylated CAT-1 is a more efficient receptor (Wang et al., 1996). Glycosylation is also associated with a number of receptor-mediated resistance phenotypes that have been identified for specific virus-host combinations. These restrictions include the restriction of MoMLV by M. dunni cells (Eiden et al., 1993; Eiden et al., 1994), restriction of E-MLVs by Chinese hamster and rat cells (Miller and Miller, 1992;1993; Tavoloni and Rudenholz, 1997), and restriction of the MoMLV variant Spl574 by laboratory mouse cells (Yan et al., 2008). All of these restrictions are relieved by inhibitors of glycosylation.
The viral Env glycoprotein is also glycosylated. E-MLV Env has a small number of glycans compared to more complex retroviruses like HIV-1; MLVs have 7–9 N-glycans, and one site for O-linked glycosylation (Pinter and Honnen, 1988). Four N-glycan sites, gs3, 5, 7, 8, are variably present. One N-glycan site, gs2, uniquely carries an endoglycosidase H-sensitive high-mannose glycan; the others carry complex types (Kayman et al., 1991). Use of inhibitors to block N-linked glycosylation during virus replication has demonstrated that glycans are critical for the maturation and transport of Env. Inhibitors that specifically block the earliest steps in glycosylation can prevent cleavage of the Env precursor into SU and TM resulting in virions with no Env glycoprotein (Schultz and Oroszlan, 1979). There is limited data on the possible functional roles of the individual Env N-glycans. Felkner and Roth (1992) showed that loss of MoMLV gs2 resulted in temperature sensitive production of virus in Rat2 cells, and loss of gs4 from MoMLV and FrMLV produced noninfectious virus lacking SU protein; the gs4 mutation inhibited processing and prevented incorporation of Env into virus, although it was also shown that a glycan at this site is not required for Env function (Felkner and Roth, 1992; Kayman et al., 1991; Li et al., 1997).
In this study we assess the entry properties of 3 different E-MLV isolates mutagenized to eliminate one of three N-glycan sites: gs1 and gs2, the two glycan sites in the RBD, and gs8, located in a region of the C-terminal domain of SU known to mediate post-receptor binding fusion (Burkhart et al., 2005). We characterize these viruses for their ability to mediate binding and entry with 4 naturally occurring CAT-1 variants found in different rodent species.
We used site-specific mutagenesis to remove individually one of three sites for N-linked glycosylation from three E-MLVs: MoMLV, FrMLV, and AKV MLV (Fig. 1A). Two sites (gs1, gs2) are located in the 236 amino acid receptor binding domain (RBD) for all three viruses, whereas a third site, gs8, is found in MoMLV and FrMLV but not AKV MLV. This third site maps to a region that has been implicated in post-binding cell fusion (Burkhart et al., 2005), and provides the epitope for the GIX cell surface marker (Rosner et al., 1980). Single glycosylation site mutants were generated for all 3 sites in MoMLV, for the two RBD sites in AKV MLV, and gs1 was removed from FrMLV.
Western analysis of virus infected NIH 3T3 cells with anti-gp70 shows that, as expected, removal of these sites reduces the size of the Env precursor, gPr80Env, as well as the size of the processed SU for all 6 mutants (Fig. 1B). We also used the MoMLV Env-specific antibody MAb538 to analyze the three MoMLV mutants; MAb538 reacts with gPr80Env but not SU on western blots. MAb538 detects MoMLV and Mo-gs2, but not Mo-gs1 or Mo-gs8 (Fig. 1B), indicating that removal of individual glycosylation sites alters the immunogenicity of this glycoprotein.
We tested the mutant viruses for infectivity on cells of 2 Mus species and 2 non-Mus rodent species with functional, but variant CAT-1 receptors. These 4 receptors differ in their third extracellular loop that carries critical residues for entry (Fig. 2A). dCAT-1 from the Asian M. dunni mouse differs from laboratory mouse mCAT-1 at two sites in this loop. The hamster choCAT-1 and rat xcCAT-1 genes have several substitutions relative to mCAT-1 and have additional residues flanking one of the two glycosylation sites in this loop; rat xcCAT-1 also lacks one of the 2 glycosylation sites (Kubo et al., 2002). Chinese hamster cells are normally resistant to infection by ecotropic MLVs, but this restriction is mediated by glycosylation (Miller and Miller, 1992) and is relieved in Chinese hamster Lec8 cells (Miller and Miller, 1993), a line that lacks GlcNAc-transferase I (Gottlieb et al., 1975), an enzyme involved in N-glycan processing.
Susceptibility to 3 ecotropic MLVs and glycosylation mutants was assessed by the XC overlay test (Fig. 2B). MoMLV is restricted in both of the M. dunni-derived cell lines used here; these lines were derived from tail cells cultured from a single mouse (Lander and Chattopadhyay, 1984), but cells were subsequently distributed to different laboratories and now differ in the extent of their resistance to MoMLV. Removal of the gs8 site from MoMLV had no clear effect on the ability of this MoMLV derivative to infect cells carrying mCAT-1, dCAT-1 or choCAT-1. Removal of gs1 and gs2, however, produced viruses that efficiently infected NIH 3T3 cells and XC cells, but were substantially less infectious for M. dunni, MDTF and Lec8 cells than wild type MoMLV. On M. dunni cells, no XC plaques were produced by either Mo-gs1 or Mo-gs2 in 7 of 8 tests, a million-fold reduction compared to the infectivity of these viruses on NIH 3T3, and a reduction of over 100-fold relative to the infectivity of MoMLV in these same cells. We also tested these viruses on MDTF cells, which are more sensitive than M. dunni cells to MoMLV (Fig 2B); replication of the MoMLV mutants was detected, but was reduced at least 1000-fold compared to wild type MoMLV.
AKV MLV and FrMLV57 show small differences in infectivity for NIH 3T3 and M. dunni mouse cells (Fig. 2B). Removal of either gs1 or gs2 from AKV MLV produced viruses showing no differences in infectivity on these cells. However, as was seen for Mo-gs1, Fr-gs1 was substantially less infectious than wild type FrMLV57 for M. dunni cells; this is also consistent with a previous observation that removal of both RBD glycan sites from FrMLV results in reduced infectivity on M. dunni cells (Battini et al., 1994). These results taken together indicate that removal of specific N-glycan sites from the MLV envelope can produce detectable changes in immunogenicity, and that glycans in the RBDs of MoMLV and FrMLV, but not AKV MLV, are needed for efficient entry into cells with 2 of the receptor variants tested here, dCAT-1 and choCAT-1.
To determine if the viruses that showed altered infectivity after removal of specific N-glycan sites would also show altered infectivity if Env N-oligosaccharides were modified by type or size, we used two methods to generate viruses with altered glycans. First, we collected virus grown in Lec8 cells. Because of the absence of GlcNAc-transferase I, Lec8 cell glycoproteins carry smaller Man5(GlcNAc)2-oligosaccharides and the SU glycoproteins produced in these Lec8 cells are markedly reduced in size (Fig. 3A). Second, we treated MLV infected cells with 4 inhibitors of glycosylation to produce viruses with other types of altered N-glycans (Fig. 3B). The inhibitors specifically block different enzymes involved in N-glycosylation: CST blocks glucosidase I and generates immature Glc3Man7–9 oligosaccharides; DNM blocks glucosidase II and produces Glc1–2Man7–9 structures; DMM blocks mannosidase I and produces Man7–9(GlcNAc)2 structures that lack glucose. All three of these inhibitors thus produce high mannose structures. Sw blocks mannosidase II and inhibits formation of N-linked complex oligosaccharides, so hybrid structures are produced (Man4GlcNAc2, Man5GlcNAc2, hybrid chains). Western analysis indicates that the early glycosylation blocks by CST or DNM treatment increase the size of gPr80env and reduce processing into SU. SU produced in the presence of DMM, DNM and Sw is decreased in size (Fig. 3B). gPr80Envs produced in the presence of the various inhibitors are all identified by MAb538 by Western immunoblotting, except for the CST-modified glycoprotein (Fig. 3B).
FrMLV and MoMLV stocks produced in Lec8 cells or in the presence of DMM, DNM and Sw were tested for relative infectivity on M. dunni and NIH 3T3 cells. All stocks tested contained infectious virus. All of the FrMLVs showed comparable infectivity on the two cells by the XC overlay test (data not shown). All of the MoMLVs showed greater infectivity on NIH 3T3 cells compared to M. dunni, within the range typically observed for MoMLV (data not shown). This suggests that Env glycan type or size does not influence receptor usage of E-MLVs.
To determine if the observed differences in infectivity with the deglycosylated viruses could be attributed to the CAT-1 receptor variants in these cells, the viruses were used to infect stable transfectants expressing dCAT-1 or mCAT-1 (Fig. 4). M. dunni cells expressing mCAT-1 in addition to endogenous dCAT-1 showed increased infectibility with MoMLV but infectibility of these cells with FrMLV was not altered, as previously shown (Yan et al., 2008). These cells also showed increased infectibility with Mo-gs1, Mo-gs2, and Fr-gs1. Thus, the expression of mCAT-1 in M. dunni cells resulted in virus-specific increased susceptibility to Fr-gs1 and to the 3 MoMLV variants.
Expression of mCAT-1 or dCAT-1 in MA139 ferret cells renders them susceptible to E-MLV infection (Yan et al., 2008) (Fig. 4). As previously noted, ferret cells expressing mCAT-1 (FerrM) and dCAT-1 (FerrD) are equally infectible by MoMLV indicating that the sequence difference between mCAT-1 and dCAT-1 does not account for the MoMLV susceptibility difference between NIH 3T3 and M. dunni cells (Yan et al., 2008). However, while FerrM was equivalently susceptible to MoMLV, Mo-gs1 and Mo-gs2, FerrD was nearly 1000-fold less susceptible to Mo-gs1 and Mo-gs2. We also infected a ferret transfectant expressing dCAT-1 from which the two N-glycan sites had been deleted by mutagenesis (Fig. 2A) (Yan et al., 2008). The cells expressing deglycosylated dCAT-1-g showed the same pattern of virus susceptibility as FerrD (data not shown).
These same ferret transfectants were infected with FrMLV and Fr-gs1. Fr-gs1, like Mo-gs1, showed reduced infectivity in the transfectants expressing dCAT-1 (Fig. 4) and dCAT-1-g (data not shown) compared to the mCAT-1 transfectant. These results indicate that while the CAT-1 sequence difference does not account for the resistance of M. dunni to MoMLV, this sequence difference is an important factor that influences susceptibility to the deglycosylated viruses. Removal of gs1 from FrMLV and either RBD glycan from MoMLV compromises the ability of these MLVs to interact with the dCAT-1 receptor, but not with the mCAT-1 receptor whether the receptor is expressed in mouse cells, or cells of heterologous species.
The reduction in susceptibility to deglycosylated MoMLV and FrMLV in dCAT-1 versus mCAT-1 expressing ferret cells is not as dramatic as the susceptibility differences between M. dunni and NIH 3T3. This suggests that other factors may contribute to the resistance of M. dunni cells. Because glycosylation contributes to MoMLV resistance in dunni cells (Eiden et al., 1994; Yan et al., 2008), we tested the effects of inhibitors of glycosylation on infectivity of deglycosylated viruses in M. dunni cells (Table 1). All inhibitors tested increased susceptibility to MoMLV as well as to Mo-gs1, Mo-gs2, and Fr-gs1, but not to FrMLV57. Thus, glycosylation is a contributing factor to restriction of Fr-gs1 and all of the MoMLV variants in M. dunni cells.
To determine if removal of envelope glycans affects later stages in the viral life cycle, we infected cells with pseudotypes carrying the LacZ reporter gene. Pseudotypes were generated for MoMLV, FrMLV57, and their respective gs1 mutants.
As observed previously, the MoMLV pseudotype is restricted in M. dunni cells although these cells are susceptible to infection with the FrMLV57 pseudotype (Table 2) (Yan et al., 2008). M. dunni cells showed no detectable infection with pseudotypes of either Mo-gs1 or Fr-gs1, consistent with the XC overlay test results (Fig. 2B).
The transfected MA139 line carrying mCAT-1, FerrM, was efficiently infected by all 4 pseudotypes as also shown by the XC test (Fig. 4). In contrast, FerrD cells expressing dCAT-1 resemble FerrM in their susceptibility to FrMLV and MoMLV pseudotypes, but show a 60–100-fold reduction in sensitivity to pseudotypes of both gs1 mutants (Table 2). These results are consistent with the XC test results and indicate that the restriction of viruses lacking specific glycans is receptor mediated.
To determine if the removal of glycans affects virus binding to the CAT-1 receptor or a later stage in the entry process, we performed virus binding assays on NIH 3T3, M. dunni and MDTF cells with MoMLV, FrMLV57, and their respective gs1 glycosylation mutants. We also used the ferret transfectants FerrM and FerrD to evaluate binding of FrMLV57 and Fr-gs1.
Binding of each virus was initially assessed on NIH 3T3 cells (Fig 5A, B). Strong virus binding was observed on NIH 3T3 cells for both wild type viruses, and removal of glycosylation sites only slightly modulated the virus-binding characteristics in a virus-specific manner. Mo-gs1 had a slightly decreased binding efficacy compared to MoMLV (Fig. 5A) whereas Fr-gs1 showed slightly increased binding (Fig. 5B), reflecting the different XC plaque titers produced by these virus stocks on NIH 3T3 (Fig. 2B).
Virus binding properties were drastically different on M. dunni (data not shown) and MDTF cells (Fig 5C, D), which showed nearly identical properties. Binding of MoMLV and Mo-gs1 were undetectable on M. dunni and MDTF cells (Fig. 5C), consistent with the poor infectivity of both viruses on these cells. Binding of FrMLV57 was detected on both dunni cell lines but was reduced compared to NIH 3T3 cells. This difference did not correlate with the XC plaque titer of this virus which was 5–10 fold higher on M. dunni than on NIH 3T3 cells suggesting that other cell-specific factors may influence virus replication. In contrast to wild type FrMLV57, Fr-gs1 had no detectable binding ability on either M. dunni or MDTF cells (Fig 5D). These data suggest that removal of gs1 from FrMLV57, a virus capable of infecting M. dunni cells, drastically reduces its ability to recognize the dCAT-1 receptor, preventing efficient replication on these cells.
Virus binding of FrMLV57 and Fr-gs1 was also assessed on FerrM and FerrB cells. Both viruses showed reduced but equivalent binding to FerrM cells (Fig. 5E), consistent with the observation that these cells are less susceptible to MLVs than mouse cells (Fig. 4). FerrD resembled FerrM in its ability to bind FrMLV57, but showed very reduced binding with Fr-gs1 (Fig. 5F). These data suggest that receptor polymorphism is responsible for reduced infectivity of the gs1 mutants.
Among the various factors responsible for intrinsic resistance to retroviruses are several that inhibit virus entry. Such factors include ERV produced Env glycoproteins that interfere with virus-receptor binding, and mutations in receptor genes that alter their interactions with viruses (Stocking and Kozak, 2008). For the MLVs, receptor polymorphisms that recognize different Env variants have been identified for the XPR1 receptor that mediates infection of xenotropic and polytropic MLVs (XMVs and PMVs) (Marin et al., 1999; Yan et al., 2007), viruses originally described as different host range groups (Hartley et al., 1977). For the E-MLVs, there are fewer examples of receptor mediated host range variations, in part because, unlike the X/PMVs that can infect all mammals tested, E-MLV infection is limited to rodents. In addition, most known examples of E-MLV entry restrictions are due, at least in part, to glycosylation of cellular proteins. Because glycosylation of the viral Env has also been associated with altered infectivity of multiple viruses including MLVs (Battini et al., 1994) and other retroviruses (Ogert et al., 2001), we examined the need for specific N-glycans in Env regions important for entry. Here we show that deletion of specific N-glycan sites from ecotropic MLV env genes produces viruses with altered host range. This effect is receptor variant specific, virus type specific, and glycan site specific. Deletion of glycans gs1 and gs2 alters the host range of MoMLV and FrMLV but not AKV MLV, and this restriction is observed only in cells expressing two of the four naturally occurring CAT-1 variants.
Virus infectivity was shown to be affected by glycan site deletion but not by the alteration of glycan type or size. This suggests that the role of glycans in host range may be a consequence of their cotranslational placement on the Env protein, a process that may result in conformational changes in the Env glycoprotein that differentially alters recognition by the 4 receptor variants. Glycan additions generally ensure proper folding of proteins, help maintain their stability, and are needed for intracellular routing and further processing. For MLV Env, blockage of glycosylation by tunicamycin halts processing and translocation to the plasma membrane (Schultz and Oroszlan, 1975). That glycan removal affects infectivity due to altered folding rather than a specific role for glycans in entry is consistent with the crystal structure of FrMLV (Fass et al., 1997) which shows that neither gs1 nor gs2 is positioned near the residues in VRA that form the pocket for virus binding, namely residues S84, D86 and W102 (Davey et al., 1999).
Deletion of either of the 2 RBD glycan sites of MoMLV and gs1 of FrMLV produces viruses that identify a functional distinction among the 4 naturally occurring CAT-1 variants. These mutated viruses are much less able to infect dunni and hamster cells, while infectivity on rat XC cells and NIH 3T3 cells is not altered. These infectivity differences are also seen in MA139 ferret cells expressing the dCAT-1 and mCAT-1 receptor variants indicating that receptor polymorphism is the major responsible host cell factor. Virus binding assays further demonstrate there is an association between infectivity and the binding ability of Fr-gs1 compared to the wild type FrMLV in M. dunni cells as well as in the transfected ferret cells. Infectivity can be, however, affected by other, secondary cellular factors as shown by the fact that glycosylation inhibitors can reduce this entry restriction in M. dunni cells.
The three viruses used here have ecotropic host range, but show significant sequence variation in their RDBs and also differ in their patterns of glycosylation (Fig. 1A). FrMLV and MoMLV are laboratory isolates that, unlike AKV, are oncogenic in mice. Both viruses were isolated after serial passage of tumors in mice and neither of these viruses have counterparts that are found naturally in laboratory mice or wild mouse species. In contrast, AKV MLVs are naturally occurring MLVs carried as endogenous retroviruses by many inbred laboratory strains and are also found in Asian species of Mus (Kozak and O’Neill, 1987). Sequence comparisons of their Env genes indicate closer relationship between FrMLV and MoMLV (88% amino acid identity in RBD) than between either of these viruses and AKV (78% identify). These similarities are reflected in our results; removal of RBD glycan sites from FrMLV and MoMLV similarly compromises infectivity in cells expressing dCAT-1, whereas AKV mutants show no changes in infectivity. Examination of other MLV isolates indicates there is naturally occurring polymorphism at 4 glycan sites (gs3,5,7,8) among ecotropic viruses suggesting that these sites are not critical for producing infectious virus as shown here for gs8.
Mutational changes in the retroviral RBD can lead to the generation of viruses that can use alternative receptors, multiple receptors or even multiple determinants on the same receptor protein (Stocking and Kozak, 2008). The coevolution of Env and receptor has resulted in their sequence diversity and functional plasticity, and previous studies have identified sequence variations in E-MLVs responsible for variations in receptor usage (Yan et al., 2008; Masuda et al, 1996). Such variants show altered infectivity into different mouse or hamster cells, and differential ability to induce multinucleated syncytia. The present study indicates that RBD glycans also serve to control virus host range variation, a control that likely depends on protein folding rather than a direct role in receptor interaction.
Two ecotropic MLV isolates were obtained from J. W. Hartley (NIAID, Bethesda, MD): AKV MLV and Moloney MLV (MoMLV). We also produced stocks of Friend and MoMLV from molecular clones of FrMLV57 and MoMLV (pNCA-HA) obtained from S. Ruscetti (NCI, Frederick, MD) (Oliff et al., 1980) and J. Silver (NIAID, Bethesda, MD) (Ou et al., 2006).
Virus stocks were made by collecting culture fluids from infected or transfected cells. Infectious virus in these stocks was quantitated by the XC overlay test (Rowe et al., 1970; Yan et al., 2008) following infection of NIH 3T3 and 2 lines derived from tail cells of a single M. terricolor (M. dunni) mouse; the two cell lines are here designated M. dunni and MDTF (Lander and Chattopadhyay, 1984). The XC overlay test was also used to assess virus susceptibility on Chinese hamster E36 (Gillin et al., 1972) and Lec8 cells (ATCC CRL-1737), XC rat cells, and MA139 ferret or M. dunni cells stably transfected with 3 CAT-1 receptor variants: mCAT-1 from NIH 3T3, dCAT-1 from M. dunni, and dCAT-1-g, a variant of dCAT-1 from which both glycosylation sites were removed by mutagenesis (Yan et al., 2008). Western analysis indicates equivalent expression of mCAT-1 and dCAT-1 in the ferret transfectants (Yan et al., 2008)
Subconfluent cultures were infected with appropriate dilutions of virus stocks in the presence of polybrene (4 ug/ml; Aldrich, Milwaukee, WI). The cells were irradiated 4 days after virus infection with ultraviolet light from germicidal bulbs (30 sec at 60 ergs/mm2), and were then overlaid with XC cells. XC cells produce plaques of multicleated syncytia in response to focal areas of virus-infected cells. Cultures were fixed and stained 3 days later and examined for plaques of syncytia. Virus spread was poor in infected XC and Lec8 cells making XC plaques difficult to read, so infected cells were first irradiated and overlaid with NIH 3T3 or H3T3-1, a line derived in this laboratory from tail cells of M. spicilegus; these cells do not restrict any ecotropic MLVs. After 3 days, cells were again irradiated and overlaid with XC cells.
Cells were treated by various inhibitors of N-linked glycosylation as follows: deoxymannojirimycin (DMM, 100 ug/ml); castanospermine (CST, 100 ug/ml), tunicamycin (Tu, 0.2 ug/ml); 2-deoxy-D-glucose (2DG, 25 mM) deoxynojirimycin (DNM, 200 ug/ml); swainsonine (Sw, 200 ug/ml). All inhibitors were obtained from SIGMA (La Jolla, CA). Inhibitors were added to cultures that had been seeded the previous day and were not removed when virus and polybrene were added 18–24 hours later. Cells were lysed for Western immunoblotting 3–4 days later or were irradiated for the XC overlay test. To generate viruses with altered N-glycans, inhibitors were added to virus producing cells. Medium was replaced the next day and fresh inhibitor was added. Virus was collected 3–4 days later.
MoMLV, FrMLV57 and their respective gs1 mutants were used to infect an NIH 3T3 derived cell line stably transfected with pCLMFG-LacZ (Imgenex Co., San Diego, Calif.) and kindly provided by Dr. J. Silver (NIAID, Bethesda, MD). Filtered media from the virus infected cultures contained a mixture of infectious virus and LacZ pseudotypes. Cells were infected with appropriate dilutions of these pseudotype virus stocks in the presence of 4–8 μg/ml polybrene. One day after infection, cells were fixed with 0.4% glutaraldehyde and assayed for β-galactosidase activity using as substrate 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal, 2 mg/ml; ICN Biomedicals, Aurora, Ohio). Infectious titers were expressed as the number of blue cells per 100 microliters of virus supernatant.
Six mutant viruses lacking glycosylation sites gs1, gs2, or gs8 (Fig. 1A) were produced by overlapping PCR using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA). MoMLV mutants Mo-gs1, Mo-gs2 and Mo-gs8 were generated using clone pNCA-HA (obtained from J. Silver, NIAID, Bethesda, MD) (Ou et al., 2006). AKV MLV mutants AK-gs1 and AK-gs2 were made using clone pGEM-AKV, containing the PstI fragment of AKV MLV from pAKV23 (obtained from J. Lenz, Albert Einstein College of Medicine, Bronx, NY). FrMLV mutant Fr-gs1 was produced using the FrMLV57 clone (obtained from S. Ruscetti, NCI, Frederick, MD) (Oliff et al., 1980).
For each mutagenesis, sense and antisense primers containing the various mutations were used in PCR reactions with flanking 5′ and 3′ primers as indicated in Table 3. The two products of these reactions were combined and used as template for a second PCR using the flanking primers. MoMLV PCR products lacking gs1 or gs2 were digested with PmlI and NsiI and ligated into pNCA-HA; the MoMLV fragment lacking gs8 was introduced as an HpaI fragment. The Fr-gs1 mutant was introduced into FrMLV57 as a SacII insertion. The AK-gs1 and AK-gs2 mutants were generated as follows: a 2.7 kb BsmI fragment was recovered from pGEM-AKV using forward primer: 5′-GCAGGAGTGAGAGTACGAGGACAT and reverse primer 5′-TGGACCAGGCGATTGAGAA and cloned it into the vector pCR2.1-TOPO. Overlapping PCR was performed using indicated primers and the BsgI/AvrII fragments of the PCR product were cloned into the AKV subclone. Two 2.7 kb BsmI fragmentsfrom these subclones containing N43Q and N199Q were ligated into pGEM-AKV. All mutations were confirmed by sequencing; all clones of AK-gs1 and AK-gs2 contained the additional mutation Q341H. Clones were transfected into NIH 3T3 cells and culture medium containing virus was collected.
Lysates from virus infected cells were tested for viral Env by western immunoblot analysis. Cell lysates were subjected to electrophoresis on NuPAGE 4–12% Bis-Tris gels (Invitrogen, Carlsbad, CA). Western blots were probed with two antibodies against MLV Env: a goat polyclonal anti-Rauscher MLV gp70 antiserum (ViroMed Biosafety Laboratories, Camden, NJ) with peroxidase conjugated rabbit anti-goat IgG as the secondary antibody (Invitrogen, Eugene, OR), and MAb538, a monoclonal anti-MoMLV antibody (Lander et al., 1984) provided by J. Portis (NIAID, Hamilton, MT) with peroxidase-conjugated goat anti-mouse IgG (Invitrogen).
The virus binding assay was performed essentially as described previously (Kadan et al., 1992)]. NIH 3T3, M. dunni, MDTF and tranfected ferret cells were detached from culture flasks by a short treatment at room temperature with trypsin-EDTA (Gibco, Grand Island, NY). They were washed once and resuspended at 2 million cells per ml. One million cells were incubated in presence of 100 μl of virus suspension and 8 μg/ml polybrene for 40 minutes at 37°C in a final volume of 1 ml. Cells treated similarly in the absence of virus stock served as negative control. Samples were mixed every 10 minutes. At the end of the incubation, cells were washed twice with wash buffer (PBS supplemented with 1% bovine serum albumin). Virions bound to the cells were detected by staining the cells first with the goat-anti gp70 antiserum (1:200) in 100μl for 30 minutes at 4°C. After two washes with wash buffer, cells were incubated with Alexa Fluor 488 labeled donkey anti-goat IgG (Invitrogen, Carlsbad, CA)(1:500) in 100μl for 30 minutes at 4°C. After two additional washes, cells were resuspended in 500μl PBS. To exclude dead cells from the analysis, the cell suspension was incubated at room temperature for 10 minutes with 20 μl 7-AAD solution (BD Via Probe, BD Biosciences, San Jose CA) before being analyzed by flow cytometry (FACScalibur, BD Biosciences Immunocytometry Systems). Fluorescence data of a minimum of 50,000 cells were recorded and analysis was performed on live cells (7-AAD negative cells, >90% of the population) using Cell Quest Pro (BD Biosciences).
This research was supported by the Intramural Research Program of the NIH, NIAID.
We thank Qingping Liu for expert technical assistance and Alicia Buckler-White for sequencing.
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.