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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Dent Mater. Author manuscript; available in PMC 2010 October 1.
Published in final edited form as:
PMCID: PMC2731812

Phosphoric acid esters cannot replace polyvinylphosphonic acid as phosphoprotein analogs in biomimetic remineralization of resin-bonded dentin


Polyvinylphosphonic acid (PVPA), a biomimetic analog of phosphoproteins, is crucial for recruiting polyacrylic acid (PAA)-stabilized amorphous calcium phosphate nanoprecursors during biomimetic remineralization of dentin collagen matrices. This study tested the null hypothesis that phosphoric acid esters of methacrylates in dentin adhesives cannot replace PVPA during bimimetic remineralization of resin-dentin interfaces. Human dentin specimens were bonded with: I) XP Bond, an etch-and-rinse adhesive using moist bonding; II) XP Bond using dry bonding; and III) Adper Prompt L-Pop, a self-etching adhesive. The control medium contained only set Portland cement and a simulated body fluid (SBF) without any biomimetic analog. Two experimental Portland cement/SBF remineralization media were evaluated: the first contained PAA as the sole biomimetic analog, the second contained PAA and PVPA as dual biomimetic analogs. No remineralization of the resin-dentin interfaces could be identified from specimens immersed in the control medium. After 2–4 months in the first experimental medium, specimens exhibited either no remineralization or large crystal formation within hybrid layers. Only specimens immersed in the second remineralization medium produced nanocrystals that accounted for intrafibrillar remineralization within hybrid layers. The null hypothesis could not be rejected; phosphoric acid esters in dentin adhesives cannot replace PVPA during biomimetic remineralization of adhesive-bonded dentin.

Keywords: Biomimetics, Phosphate esters, Dentin adhesive, Remineralization, Intrafibrillar, Interfibrillar

1. Introduction

The advent of new generations of dentin adhesives and bonding techniques has improved the quality of resin-dentin bonds [1,2]. However, degradation of collagen fibrils by endogenous matrix metalloproteinases (MMPs) [3] in hybrid layers of resin-bonded interface over time remains a vexing problem [46]. This probably accounts for the reduced longevity of clinically-applied adhesive restorations [7,8], which results in billions of dental care dollars spent annually on replacement of these restorations. Although the integrity of hybrid layers may be preserved by application of MMP inhibitors such as chlorhexidine as part of the dentin bonding procedures [36], a zone of resin-sparse, demineralized dentin inadvertently remains that is potentially susceptible to creep rupture [9] or cyclic fatigue rupture [10] after prolonged intraoral function. In addition, it remains to be determined whether MMP-inhibiting activity of chlorhexidine is provisional or permanent (i.e. delaying instead of arresting hybrid layer degradation) as it is water-soluble and may eventually leach from hybrid layers. Thus, there is a compelling need to pursue alternative methods for extending the longevity of resin-dentin bonds.

Biomimetic remineralization offers the potential for remineralizing incompletely-infiltrated hybrid layers [11]. A Portland cement-based [12] biomimetic remineralization protocol that involves the use of dual biomimetic analogs has succeeded in inducing interfibrillar and intrafibrillar remineralization of acid-etched dentin [13] and incompletely resin-infiltrated hybrid layers created by etch-and-rinse adhesives [11]. To promote the remineralization of the imperfectly-created resin-dentin interfaces, a low molecular weight polyacrylic acid (PAA) was used to mimic the function of dentin matrix protein-1 (DMP-1) on stabilizing amorphous calcium phosphate precursors [14] and reducing the dimension of these liquid phase precursors to a nanoscale [15]. The other biomimetic analog, polyvinylphosphonic acid (PVPA), is a polyanion that mimics the negative charges of phosphoproteins such as DMP-1, phosphophoryn or bone sialoprotein [16,17]. Similar to phosphoprotein molecules, PVPA is conjectured to bind to demineralized collagen fibrils, thereby guiding the deposition of PAA-stabilized amorphous calcium phosphate nanoprecursors within and along specific sites of the collagen fibrils.

In an effort to develop effective restorative materials which enable demineralized dentin to be remineralized, glass-ionomer cements were thought to accomplish this objective due to their long-term fluoride release [18,19]. However, remineralization sites were restricted to the dentin surface [20,21]. Moreover, their poor wear resistance, brittleness, and low bond strength limited their clinical use. Hashimoto et al. [22] recently reported that crystals were formed in 40-μm thick gaps between fluoride-releasing resin adhesives and dentin after long-term water storage. Hayakawa et al. [23] also reported deposition of carbonated apatite on polymer disks created from a phosphorylated dentin bonding agent (Clearfil Photo Bond, Kuraray Medical Inc., Tokyo, Japan) after these disks were immersed in an electrolyte solution. The authors opined that the phosphonic acid and phosphate groups in the phosphorylated dentin bonding agent had the capability to induce nucleation and growth of apatite crystals. The results of these two studies were reminiscent of dystrophic calcification that was recently reported in intraocular lens [2426]. Nevertheless, the ability for phosphorylated dentin bonding agents to induce apatite formation may create a window of opportunity for utilizing phosphate ester resin monomers as substitutes for PVPA as biomimetic analogs for dentin remineralization. As these resin monomers are applied to dentin and polymerized in-situ, they are located directly adjacent to the demineralized collagen matrix so that it is not necessary for them to diffuse from the external environment into resin-sparse regions of the resin-infiltrated dentin. Polymerized adhesives containing hydrophilic resin derivatives are also permeable to calcium and hydroxyl ions which are necessary to remineralize dentin.

Thus, the objective of this study was to examine whether dentin adhesives containing phosphoric acid esters of methacrylates can replace the use of PVPA for inducing intrafibrillar and interfibrillar remineralization of collagen fibrils within hybrid layers. The null hypothesis tested was that phosphoric acid esters present in etch-and-rinse and self-etching adhesives cannot replace PVPA as biomimetic analogs during remineralization of resin-dentin interfaces.

2. Materials and methods

2.1 Dentin adhesives

Two phosphoric acid ester-containing adhesives were used in the present study. The first adhesive was XP Bond (Dentsply De Trey, Konstanz, Germany). It is a tertiary butanol-based, filled, etch-and-rinse adhesive. It contains dipentaerythritol penta-acrylate phosphate (PENTA) as an adhesion promoter. The second adhesive was Adper Prompt L-Pop (3M ESPE, St. Paul, MN, USA). It is a self-etching adhesive which contains methacrylated phosphoric acid mono-and diesters [27]. The phosphoric acid and methacrylate group in these acidic resin monomers are combined into one molecule that etches and primes simultaneously.

2.2 Specimen preparation

Forty-five recently extracted noncarious human third molars were collected after patients’ informed consents were obtained under a protocol approved by the Human Assurance Committee of the Medical College of Georgia. A flat dentin bonding surface in mid-coronal portion of tooth was prepared perpendicular to the longitudinal axis of each tooth using a slow-speed Isomet diamond saw (Buehler Ltd, Lake Bluff, IL, USA) under copious water cooling. Each dentin surface was then polished with a 400-grit silicon carbide paper under running water. The specimens were randomly divided into three groups (N=15): I) XP Bond, the etch-and-rinse adhesive using a moist bonding technique; II) XP Bond using a dry bonding technique; and III) Adper Prompt L-Pop, the self-etching adhesive.

For XP Bond, each dentin surface was etched with 32% phosphoric acid gel (Uni-Etch, Bisco Inc. Schaumburg, IL, USA) for 15 sec and thoroughly rinsed with deionized water. For group I, a moist bonding technique was employed by keeping the acid-etched dentin visibly moist during bonding. For group II, the etched dentin surface was air-dried with 30 psi compressed air for 5 sec. The latter was applied at 5 mm away from the dentin surface. XP Bond was then applied to the dentin surface using two adhesive coats and light-cured for 20 sec using a light-curing unit (LEDemetron 1, Kerr Corp., Danbury, CT, USA).

For Adper Prompt L-Pop, the two components of this one-step self-etching adhesive were mixed according to manufacturer’s instruction, applied to the dentin surface, and agitated for 15 sec. The liquid was then gently air-dried and spread into a homogenous, slightly shiny film and light-cured for 20 sec.

Incremental placement of two 2-mm thick layers of a resin composite was done and light-cured separately for 40 sec each. The bonded teeth were stored at 100% relative humidity for 24 hrs. Each tooth was then sectioned occluso-gingivally into 1-mm thick slabs, each containing the resin-dentin interface.

2.3 Remineralization medium

Type I white Portland cement (Lehigh Cement Company, Allentown, PA, USA) was sieved and mixed with deionized water in a water-to-powder ratio of 0.35:1, placed in flexible silicone molds and allowed to set and age at 100% relative humidity for one week before use.

A simulated body fluid (SBF) was prepared by dissolving 136.8 mM NaCl, 4.2 mM NaHCO3, 3.0 mM KCl, 1.0 mM K2HPO4·3H2O, 1.5 mM MgCl2·6H2O, 2.5 mM CaCl2 and 0.5 mM Na2SO4 in deionized water [28] and adding 3.08 mM sodium azide to prevent bacterial growth. This SBF also served as the control remineralization medium, which contained no biomimetic analog. Two experimental remineralization media were prepared. For the first medium, 500 μg/mL of PAA (MW 1,800; Sigma-Aldrich, St. Louis, IL, USA) was added to the SBF as the sole biomimetic analog. For the second remineralization medium, the SBF was supplemented with 500 μg/mL of PAA and 200 μg/mL of PVPA (MW 24,000; Sigma-Aldrich) as dual biomimetic analogs. All solutions were buffered to pH 7.4 with 0.1 M Tris Base or 0.1 M HCl and filtered through 0.22 μm Millipore filters.

2.4 Biomimetic remineralization

Each specimen slab was placed over a set Portland cement block (ca. 1 g) inside a glass scintillation vial. The latter was filled with 15 mL of solution. The specimens of each group were randomly divided into three subgroups according to solution that was placed inside the vial: SBF (control medium), SBF with PAA (first experimental remineralization medium), and SBF with PAA and PVPA (second experimental remineralization medium). The glass vials were stored at ambient temperature (ca. 25°C.) The medium was changed every month. The pH of biomimetic remineralization system was monitored weekly and kept above 9.25 [29]. Specimens were retrieved after 2 and 4 months (N=5) for ultrastructural examination of the extent of remineralization.

2.5 Transmission Electron Microscopy (TEM)

The specimens were fixed in Karnovsky’s fixative and post-fixed in 1% osmium tetroxide. After fixation, each specimen was rinsed three times in sodium cacodylate buffer. The specimens were dehydrated in an ascending series of ethanol (50–100%), immersed in propylene oxide as a transitional fluid and subsequently embedded in epoxy resin. Non-demineralized epoxy resin-embedded, 90–110 nm thick sections were prepared and examined without further staining using a JEM-1230 transmission electron microscope (JEOL Inc., Peabody, MA, USA) operated at 110 kV.

3 Results

3.1 Control Medium

Control specimens of moist-bonded and dry-bonded XP Bond and those bonded with Adper Prompt L-pop examined after 2 and 4 months of immersion in SBF exhibited no remineralization within hybrid layers (not shown).

3.2 Polyacrylic acid-containing remineralization medium

Specimens that were immersed in the first remineralization medium exhibited different results among the three groups. No remineralization was apparent in the moist-bonded XP Bond specimens when PVPA was excluded from the remineralization medium (Fig. 1A). Dry-bonded XP Bond specimens had clusters of relatively large crystalline deposits within the hybrid layers (Fig. 1B). The majority of these crystalline platelets were more than 100 nm along their C-axes (Fig. 1C).

Fig. 1
TEM images taken from unstained, undemineralized sections of resin-bonded dentin that were immersed in the first experimental Portland cement/SBF remineralization medium containing PAA as the only biomimetic analog. Abbreviations: C: composite; A: adhesive; ...

Adper Prompt L-Pop bonded specimens exhibited electron-dense amorphous clusters in the interfibrillar spaces of the hybrid layers after 2 months (Fig. 1E). The amorphous clusters had converted into crystalline plates within the middle portion of the hybrid layers after 4 months (Fig. 1D). These crystalline plates were 100–150 nm along their C-axes (Fig. 1F), which were larger than the natural apatite crystallites in the underlying mineralized dentin base (Fig. 1F).

3.3 Polyacrylic acid and PVPA-containing remineralization medium

For Group I specimens bonded with XP Bond using the moist bonding technique, remineralization could be vaguely discerned from hybrid layers at 2 months (Figs. 2A,2B) and were clearly identified after 4 months (Fig. 2D) of immersion in the second remineralization medium. Regional intrafibrillar remineralization was observed in the resin-infiltrated collagen matrix without interfibrillar remineralization. The intrafibrillar minerals so formed were able to recapitulate the rope-like subfibrillar arrangement of the collagen fibrils (Figs 2C, 2E), but lacked the banded appearance that was characteristic of the mineralized fibrils from the underlying dentin base (Fig. 2E). Intrafibrillar mineral nanoplatelets in heavily remineralized collagen fibrils derived from the surface of the hybrid layer were 50–70 nm long along their C-axes (Fig. 2F).

Fig. 2
TEM of moist-bonded XP bond specimens that had been immersed in the second experimental PAA- and PVPA-containing remineralization medium. The specimens were examined after 2 months (A–C) and 4 months (D–F) of biomimetic remineralization. ...

For dry-bonded XP Bond specimens which contained collapsed resin-sparse collagen matrices, extensive remineralization of the re-expanded matrices was readily apparent even at low magnifications after the specimens were immersed for 2 months in the aqueous PAA- and PVPA-containing remineralization medium (Figs. 3A, 3B). These remineralized crystallites consisted of 50–70 nm long needles and 25–35 nm long platelets (Fig. 3C). Heavy remineralization within segregated regions of the hybrid layers was readily observed in all 4 month old specimens (Fig 3D). In those specimens, the mineral density of the remineralized part of the hybrid layer was similar to that of the underlying dentin (Fig. 3E), with a dense arrangement of interfibrillar needles and intrafibrillar platelets (Fig. 3F).

Fig. 3
TEM of XP Bond-bonded, air-dried, collapsed acid-etched dentin that re-expanded after immersion in the second experimental PAA- and PVPA-containing remineralization medium. Specimens were examined after 2 months (AC) and 4 month (DF ...

At 2 months, the Adper Prompt L-Pop bonded specimens demonstrated remineralization that commenced either from the base (Fig. 4A) or the top of the hybrid layers (not shown). Interestingly, only intrafibrillar remineralization was observed (Fig. 4B), with an orderly arrangement of nanoplatelets that revealed the pleaded, rope-like subfibrillar architecture of the collagen fibrils (Fig. 4C). After four months, the 4–6 μm thick hybrid layers were almost completely remineralized (Fig. 4D). There was minimal remineralization of the interfibrillar spaces (Figs. 4E, 4F) despite the presence of heavy remineralization within the collagen fibrils (Fig. 4F).

Fig. 4
TEM of Adper Prompt L-Pop bonded specimens that that had been immersed in the second experimental PAA- and PVPA-containing remineralization medium. The specimens were examined after 2 months (A–C) and 4 months (D–F) of biomimetic remineralization. ...

4. Discussion

The results of the present study do not warrant rejection of the null hypothesis that phosphoric acid esters present in etch-and-rinse and self-etching adhesives cannot replace PVPA as biomimetic analogs during remineralization of resin-dentin interfaces. In the first experimental group with specimens immersed in PAA-containing SBF, moist-bonded XP Bond specimens showed no remineralization. This is probably because the demineralized collagen matrix was better infiltrated with resin compared with the dry-bonded specimens and did not permit the deposition of the large crystals that were seen in the latter. Although dry-bonded XP Bond and Adper Prompt L-Pop specimens exhibited some evidences of remineralization, the crystals formed in the absence of PVPA were too large to fit inside collagen fibrils. These large crystals are more likely to occupy interfibrillar spaces. Conversely, intrafibrillar remineralization was identified from all bonded specimens after immersion in the PAA- and PVPA-containing biomimetic remineralization medium. In addition, interfibrillar remineralization was seen in the dry-bonded XP Bond specimens. Presumably, the interfibrillar spaces in the moist-bonded XP Bond and the Adper Prompt L-Pop groups were still occupied by resin. As the remineralized minerals are unlikely to displace polymerized resins, this provided a plausible explanation for the observation of intrafibrillar remineralization only in these two groups. On the contrary, the collapsed air-dried demineralized collagen matrices in the dry-bonded XP Bond specimens were resin-sparse and that the interfibrillar spaces were likely to be devoid of adhesive resin. This probably explained why both intrafibrillar and interfibrillar remineralization were identified in this dry-bonded group. Collectively, the results of this study led us to conclude that polymerized phosphoric acid esters in XP Bond and Adper Prompt L-Pop cannot replace binding of PVPA molecules on demineralized collagen fibrils (Gu et al., unpublished results) as potential phosphoprotein analogs in a biomimetic remineralization scheme.

Biomineralization is the process by which living organisms secrete inorganic minerals in the form of biominerals (e.g. magnetite, silica, oxalates, various forms of calcium carbonates, carbonated apatites) within cytoplasms, shells, teeth and bony skeletons [30]. The processes exhibit a high level of spatial and hierarchical control as mineralization usually takes place in a confined reaction environment under ambient temperature and pressure conditions. It is generally accepted that type I collagen matrix does not have the capacity to induce matrix-specific mineral formation [31] from metastable calcium phosphate solutions [32]. Although noncollagenous proteins macromolecules are known to play crucial roles in the control of nucleation and growth of the mineral phase [33,34], it was only very recently that the “bottom-up” concept of particle-mediated nanoprecursor assembly and mesocrystalline transformation [30,3537] was proposed as an alternative to the classical paradigm of “top-down” ion-mediated crystalline growth to account for the intricate biomineralization strategies identified in nature.

Noncollagenous proteins (NCPs) play critical roles in the mineralization of the predentin collagen network during dentinogenesis [17,38]. They possess carboxylic acid and phosphate functional groups that act as preferential sites for apatite nucleation [39,40]. Dentin matrix protein-1 (DMP-1) is a member of the highly acidic NCPs [41]. Its N-terminal domain is rich in aspartic acid and is thought to participate in stabilizing the amorphous calcium phosphate phase and inhibits apatite formation [17]. Its C-terminal is a glutamic acid- and serine-rich domain which has been proposed as a site that facilitates apatite nucleation. Serine molecules are potential sites for phosphorylation by casein kinases I and II [17,38]. In addition to its potent calcium binding capacity, DMP-1 has a high affinity to fibrillar collagen [42]. This complex NCP molecule has been shown to induce heterogeneous nucleation of calcium phosphate crystals and regulate crystal growth. Phosphophoryn, another phosphoprotein rich in aspartyl (Asp) and O-phosphoseryl (Ser(P)) residues, is involved in matrix-mediated biomineralization of dentin [43]. Its phosphorylation is crucial for its function as a mediator of biomineralization [44]. It is also believed to have a specific affinity for collagen [45,46] in-situ, especially with the ‘e’ band of collagen fibrils [46].

In the present study, the PVPA biomimetic molecules probably simulate the calcium phosphate binding sites of DMP-1 and act as templates for recruiting amorphous calcium phosphate nanoprecursors along the collagen microfibrils as well as on the surface of the collagen fibrils [44]. Thus, both PAA and PVPA are required for biomimetic mineralization through the particle-mediated non-classical crystallization pathway [47]. Previous studies on remineralization with analogs of dentin phosphoprotein-phosvitin [48] and casein phosphopeptides (CPP) [49] failed to demonstrate conclusively the presence of remineralized apatites in a dentin collagen matrix, in particular as intrafibrillar minerals within the collagen fibrils. When PAA was employed as the exclusive biomimetic analog in the first remineralization medium, large crystals appeared in the dry-bonded XP Bond and Adper Prompt L-Pop specimens. As there is no room inside a collagen fibril to accommodate these large crystals, the only place that they could have been deposited is either the interfibrillar spaces or the lateral branches of the dentinal tubules into which the interfibrillar spaces open. Therefore, the precipitation of large crystals either on the surface of, or within the hybrid layer is not consistent with the epitome of a biomimetic remineralization strategy. Phosphonate groups covalently linked to PEG-b-PMAA (polyethylene glycol-block-polymethacrylic acid) block copolymers resulted in the formation of barium chromate nanofibers, whereas doping of a PEG-b-PMAA solution with equal amounts of a phosphate salt (Na2HPO3) failed to reproduce such structures. This indicated that the phosphonate-functionalized block copolymer is required for morphogenic targeting instead of the simple adsorption of a single phosphoric acid functional group derived from an ionic analog [50]. In the context of the present study, although it is possible for phosphate esters of methacrylates to induce mineral precipitation, as elegantly demonstrated by Hayakawa et al. [23], these crystalline precipitates are at best conceived as dystrophic calcifications instead of true biomimetic remineralization. The results of our present work, while confirming our previous findings [13], provided new, important data demonstrating that phosphate esters from dentin adhesives cannot be used as substitutes for PVPA in biomimetic remineralization. Phosphonates are thought to be far more stable than are phosphate esters that are susceptible to the hydrolytic action of esterases. It remains to be seen whether binding of PVPA molecules to demineralized dentin and their subsequent entrapment with dentin adhesives will result in similar remineralization results. This important issue has to be investigated in future studies before the provision of a clinically-relevant remineralization scheme involving the in-situ application of biomimetic analogs can be realized.

The importance of self-assembly as a central theme was well demonstrated in our remineralization experiment. Although intrafibrillar remineralization was consistently identified, it is noteworthy that the mineral nanoplatelets were associated with the collagen microfibrils, allowing the rope-like subfibrillar architecture [51] of the collagen fibrils to be revealed. Unlike the naturally mineralized collagen fibrils from the underlying dentin base, collagen banding was not apparent in these remineralized collagen fibrils. It is tempting to attribute this phenomenon to the inability of biomimetic molecules to fully duplicate the natural biologic functions of structural phosphoprotein molecules [52]; one must admit that in comparison with the sophistication of biological self-assembly, synthetic self-assembly still operates at a relatively crude level [53,54]. However, arguing against these notions is that we were able to observe remineralized collagen fibrils with highly ordered banded appearance of the apatite platelets in experiments that involve thicker (ca. 300–1000 μm thick) layers of completely demineralized dentin (Tay, unpublished results). Presumably, the collagen fibrils were better preserved when these thick layers of dentin were demineralized in mild forms of demineralizing solutions. During the application of aggressive phosphoric acid or self-etching primers, often under continuous agitation, the collagen fibrils could have become swollen and partially-unraveled after treatments with the acid etchants and/or acidic monomers. In addition, the residual acidic monomers of the adhesives in the hybrid layer after polymerization may have an influence on the conformation of collagen. Conformational changes might involve a decrease in intermolecular hydrogen bonded amide I carbonyls associated with the α-helix structure and an enhancement of imide carbonyls hydrogen bonded to water [55], resulting in the unfolding of the α-helix structure [55,56]. These findings raise concerns on remineralization potential of caries-affected and caries-infected dentin as a minimally invasive strategy in restorative dentistry [57]. Reductions in antigenicity of type I collagen in caries-affected dentin [58] may have been caused by alterations in the collagen fibrillar conformation within the dentin organic matrix during the carious process [3,59]. Because of these subtle alterations in collagen ultrastructure, the order of apatite remineralization within hybrid layers of acid-etched normal dentin and caries-affected dentin may be different from that in natural mineralized sound dentin. This hypothesis requires validation in future studies that compare remineralization of natural caries-affected dentin with artificial carious lesions generated from sound dentin.

Based on the decline in the mechanical properties of teeth derived from patients with dentinogenesis imperfecta, Kinney et al. [60] reported that interfibrillar remineralization alone is insufficient in re-establishing the mechanical properties of the remineralized dentin to those present in normal mineralized dentin. Thus, intrafibrillar mineralization is critical in restoring the strength of dentin. As specimens moist-bonded with both the etch-and-rinse adhesive and the self-etching adhesive exhibited predominantly intrafibrillar remineralization, the contribution of intrafibrillar remineralization alone to the improvements in mechanical properties of remineralized dentin is currently unknown. Moreover, the effect of a less highly ordered type of intrafibrillar crystallite arrangement (i.e. crystallites that remineralize along pleaded but swollen collagen microfibrils, without banding) on the mechanical properties of remineralized dentin has never been investigated. These issues should be further examined using nanoscopic dynamic mechanical analysis.

5. Conclusion

Within the limits of this study, it may be concluded that:

  1. Denuded collagen matrices in resin-dentin interfaces can be remineralized in the presence of the dual biomimetic analogs polyacrylic acid and polyvinylphosphonic acid;
  2. Polymerized phosphoric acid esters in dentin adhesives cannot replace polyvinylphosphonic acid as potential phosphoprotein analogs in a biomimetic remineralization scheme;
  3. Depending upon the extent of adhesive resin infiltration into the collagen matrices, biomimetic remineralization may be manifested as either intrafibrillar remineralization alone when the interfibrillar spaces are better filled with adhesive resin, or a combination of intrafibrillar and interfibrillar remineralization when the collagen matrix is devoid of resin.


This study was supported by Grant R21 DE019213-01 from the National Institute of Dental and Craniofacial Research (PI. Franklin R. Tay). We thank Robert Smith for TEM technical assistance, Thomas Bryan for embedding of epoxy resin and Michelle Barnes for secretarial support. This revised acknowledgments had been published on line by Dental Materials: Dent Mater (2009), doi:10.1016/


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