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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Biochemistry. Author manuscript; available in PMC 2010 June 16.
Published in final edited form as:
PMCID: PMC2731577
NIHMSID: NIHMS113324

Base Excision Repair in Early Zebrafish Development: Evidence for DNA Polymerase Switching and Standby AP endonuclease Activity

Abstract

The base excision repair (BER) pathway recognizes and repairs most non-bulky lesions, uracil and abasic (AP) sites in DNA. Several participants are embryonic lethals in knockout mice. Since the pathway has never been investigated during embryogenesis, we characterized the first three steps of BER in zebrafish extracts from unfertilized eggs, embryos at different developmental stages and adults. Using a 45-mer double stranded substrate with a U/G mispair at position 21, we showed that extracts from all stages are capable of performing BER. Before 3 dpf aphidicolin-sensitive polymerases perform most nucleotide insertion. In fact, eggs and early stage embryos lack DNA polymerase-β protein. After hatching at 3 dpf, an aphidicolin-resistant polymerase, probably DNA polymerase β, becomes the primary polymerase. Previously we showed that when zebrafish AP endonuclease protein (ZAP1) level is knocked down, embryos cease dividing after the initial phase of rapid proliferation and die without apoptosis shortly thereafter. Nevertheless, extracts from embryos in which ZAP1 has been largely depleted process substrate equally as well as extracts from control embryos. Since apex1 and apex2 are both strongly expressed in early embryos relative to adults, these data indicate that both may play important roles in DNA repair in early development. In brief, the major differences in BER performed by early stage embryos and adults are the absence of DNA polymerase-β, leading to predominance of replicative polymerases, and the presence of backup Mg2+-dependent endonuclease activity in early stage embryos. The switch to normal, adult BER occurs fully when the embryos hatch from the chorionic membrane and encounter normal oxidative stress.

Keywords: base excision repair, DNA repair, uracil DNA glycosylase. AP endonuclease 1, AP endonuclease 2, DNA polymerase-β, aphidicolin, zebrafish

All cells make reactive oxygen species (ROS) as a by-product of ATP synthesis via the electron transport chain (1-3). ROS damage proteins, lipids, carbohydrates and DNA. Although the first three can be discarded and resynthesized, the cell cannot simply discard its DNA without losing vital genetic information. Therefore, all organisms have a pathway known as base excision repair (BER) to repair oxidatively damaged DNA (Figure 1). Four members of this pathway, AP endonuclease 1, XRCC1, flap endonuclease 1 and ligase I are required for embryonic development in mice (4-8). Another member of the pathway, DNA polymerase-β (pol-β), when deleted, results in either an embryonic lethal phenotype (9) or in abnormal neurogenesis and lung function, leading to neonatal death (10). Cell lines that are PolB-/- are viable but hypersensitive to methylating agents such as methylmethanesulfonate(11-13). Apex1 -/- murine embryos survive no longer than embryonic day 9 (4, 5) and no homozygous null cell lines have been cultured to date (14).

Figure 1
The DNA base excision repair pathway

Despite the importance of DNA repair in cellular physiology(15), relatively little is understood about the role of DNA repair in embryonic development. In an effort to understand the role of AP endonuclease 1 in early development, we recently explored the effects of knocking down expression of the gene in zebrafish embryos (16). Using morpholino oligonucleotides directed against the translation start site (TS-MO) to prevent translation of apex1, we demonstrated that full knockdown results in death after the rapid phase of cell division is complete, following the midblastula transition (MBT). Although zebrafish zygotic transcription begins at the MBT, failure to initiate zygotic transcription is unlikely to be the cause of death, because mouse embryos initiate zygotic transcription at the two-cell stage but Apex -/- mouse embryos do not fail until E7-E9.

Zebrafish embryos in which translation of apex1 mRNA has been blocked by means of TS-MO (full knockdown) can be rescued through the MBT by co-injection of the mRNA for WT human APEX1, although development is still not normal and the embryos die at ~7 days after fertilization (dpf). The same phenotype arises in partial knockdowns (hypomorphs) if the protein is knocked down by less than 60% by means of lower concentrations of TS-MO or if the MO targets the splice sites of apex1 (16). In an effort to examine whether full or partial knockdown of apex1 results in loss of BER, we have examined the BER pathway (Figure 1) in extracts of eggs, early embryos and adult zebrafish and in full knockdowns and hypomorphic embryos at the appropriate stage of development. We find that eggs, embryos and adult zebrafish are capable of BER. Even when ZAP1 (zebrafish AP endonuclease protein) has been reduced by >60%, embryonic extracts are still capable of repairing a U/G mispair to the same extent as extracts from controls. While adult mammalian cells have a single major AP endonuclease, encoded by Apex1, here we provide functional evidence for the presence of additional Mg2+-dependent AP endonuclease activities in zebrafish embryos, most likely AP endonuclease 2. Furthermore, the lack of DNA polymerase β in unfertilized eggs and very early stage embryos necessitates the use of other, aphidicolin-sensitive and insensitive polymerases in the pathway.

Methods

Zebrafish culture

Zebrafish were grown and embryos were spawned as described (16, 17). To prepare full knockdown and hypomorphic embryos, embryos were microinjected with TS-MO as described (16).

Preparation of extracts

Extracts were prepared from eggs or embryos at different stages by lysis in 50 mM Tris-HCl, pH 7.8, containing 200 mM KCl, 2 mM EDTA, 2 mM dithiothreitol, 0.2% NP40, 40% glycerol and protease inhibitors (Complete Mini, EDTA-free protease inhibitors, Roche Diagnostics, Indianapolis, IN)(12) followed by incubation with rotation at 4 °C for 3 h. Extracts from adult fish ground in liquid nitrogen were prepared using the same protocol. Extracts were then dialyzed overnight against 50 mM HEPES, pH 7.4 containing 0.1 mM EDTA.

BER assay

The BER assay was performed as described by Singhal et al.(18) in a 50 μl volume. The reaction mix included 50 mM Hepes, pH 7.4; 5 mM MgCl2; 1 mM dithiothreitol; 0.1 mM EDTA; 2 mM ATP; 0.5 mM NAD; dATP, dTTP and dGTP at 20 μM each; 5 mM sodium phosphocreatine; 10 units of creatine phosphokinase; 120 nM of duplex oligonucleotide; 40 μM of the fourth dNTP; and 10 μg (protein) of crude cell extract unless indicated otherwise. Reactions were incubated for 0.5 – 60 min at 22 °C and stopped by addition of EDTA to a final concentration of 83 mM. DNA was extracted with phenol-chloroform and precipitated with three volumes of chilled ethanol. Substrates and products were then resolved by means of denaturing polyacrylamide gel electrophoresis employing a 15% gel in the presence of 7 M urea and observed using phosphorImager analysis (19, 20).

In all cases we used a 45-mer double stranded oligonucleotide with a U/G mismatch at position 21 (19, 20). When uracil DNA glycosylase activity (Reaction 1) alone was measured, Substrate I, end-labeled at the 5′ end by means of polynucleotide kinase (New England Biolabs, Beverly MA) and [γ32P]ATP, was used, Mg2+ was replaced with 4 mM EDTA, and the reaction was stopped by phenol extraction. The UDG inhibitor (Ugi) was the kind gift of Dr. S Bennett (U. Oregon). When Reactions 1 and 2 were measured, Substrate I was end-labeled at the 5′ end and dNTPs were omitted from the reaction mix. When AP endonuclease activity (Reaction 2) was measured individually, substrate was prepared as described (19-21). Briefly, the 5′ end-labeled ds 45-mer was treated with Ung (1 unit/100 pmole U residues) for 30 min at 37 °C after which the Ung was heat inactivated at 70 °C for 5 min and the oligonucleotide was allowed to reanneal by slowly cooling to room temperature. When Reactions 1-3 were measured, Substrate I, end-labeled at the 5′ end, was used and either dCTP alone or all 4 dNTPs were included in the reaction mix. When Reactions 3a or b were measured separately, Substrate 1 was not end-labeled. Instead, insertion of [α32P]dCTP was measured directly by using unlabeled double stranded 45-mer and dCTP (Reaction 3a) or all 4 dNTPs (Reactions 3a and 3b). Preliminary experiments determined the amount of extract protein and time required for measuring each step in the pathway.

Quantitative real-time polymerase chain reaction (qRTPCR)

Total RNA was isolated from 60 Danio rerio embryos at 3.5 hpf or from 100 mg of freeze-dried adult fish with TRIzol Reagent from Invitrogen (Carlsbad, California) according to vendor's instructions. The RNA precipitate was resuspended in 150 μL RNase-free water and stored at −20°C. The first cDNA strand was synthesized from 1 μg total RNA in a 20-μL reaction using the High Capacity cDNA Reverse Transcription Kit from Applied Biosystems (Warrington UK) according to the manufacturer's manual. The cDNA was stored at −20°C.

For qRTPCR, the synthesized cDNA above was diluted 1:100 in RNase-free water and 4 μL was added into a 20-μL qRTPCR reaction mix containing SYBR Green PCR master mix (Applied Biosystems), 2 pmol each forward and reverse DNA primers, and water, then quantified in real time with an ABI PRISM® 7000 Sequence Detection System (Applied Biosystems) programmed thus: 1 cycle of 50°C for 2 min; 1 cycle of 95°C for 10 min; 40 cycles of (94°C for 15 s; 55°C for 30 s; 68°C for 60 s); 1 cycle of 68°C for 7 min, and 1 cycle of 4°C for 2 min. Dissociation curves were generated to ensure only one PCR product was obtained. Additionally, twelve PCR products were verified independently to contain only one product by agarose gel electrophoresis and ethidium bromide staining.

The messenger RNAs of the following genes were quantified by means of primers listed in Table 1: apex1, apex2, bactin2, aprt, ef1a, and gapdh. All measures of mRNA quantity were performed in triplicate and the data were normalized to gapdh as the other genes gave ΔCT > 4 indicating a greater than 16-fold differential in the level of expression of those genes in the 3.5 hpf embryo compared to the adult fish. Gapdh is preferred over bactin2 for normalization of gene expression in zebrafish (22).

Table 1
Selected primers for quantitative real-time PCR

Western blots

Western blots were performed as described using rabbit polyclonal antibody prepared against ZAP1 in this laboratory (16), mouse monoclonal anti Arabidopsis β-actin (GeneTex, Inc., San Antonio TX), mouse monoclonal anti rat pol-β (Thermo Fisher Scientific, Freemont CA) or rabbit polyclonal antibody prepared by 21rst Century Biochemicals, (Marlboro MA) against a peptide comprising amino acids 324 - 339 (FEYIQWKYREPKDRSE) of zebrafish pol-β.

Results

Extracts from eggs and adult fish are capable of performing BER

The individual steps in the BER pathway are outlined in Figure 1, which also details the substrates used to measure the entire pathway or individual steps. The choice of a substrate with a U/G mispair restricts the results to a uracil glycosylase-initiated BER cascade and minimizes the likelihood of confounding the data with pathways involving polynucleotide kinase and NEIL1 (23). In the first series of experiments we investigated whether extracts from eggs and adults could cleave a 45-mer ds substrate containing a U/G mispair at position 21 (Substrate I) and then insert the correct base opposite the orphan G residue. Loss of the 45-mer substrate and appearance of the 20-mer product are shown in Figure 2, Panels A and C. Cleavage represents both removal of the uracil residue (Reaction 1) and also cleavage by ZAP1 (Reaction 2). The same experiment was also performed in the presence of dCTP (Panels B and D), which is incorporated into the cleaved upstream strand (Reaction 3a). Appearance of the 21-mer in the presence of dCTP represents the first two reactions followed by insertion of deoxycytidine (21-mer, Reaction 3a). Extracts from both eggs and adults (10 μg protein from extract) were capable of removing uracil from a U-containing double stranded 45-mer, cleaving the resulting AP-site and inserting a deoxycytidine opposite the orphan G. However, the rate of cleavage followed by insertion was less in eggs than in adults. Since the level of exonuclease activity increased markedly after 15 min, subsequent assays were performed over 15 min intervals or less. Examination of individual reactions provided the rationale for the differences, as shown in the following sections.

Figure 2
BER in extracts from adult fish is more efficient than in extracts from eggs

Extracts from eggs, embryos at different stages of early development and adults recognize and remove a U residue in DNA by means of uracil DNA glycosylase (UDG)

Cleavage of the initial 45-mer requires successive activities of a uracil DNA glycosylase (UDG) and an AP endonuclcase. To understand the slower cleavage rate on the part of egg extracts compared to extracts from adult fish, we examined each step individually. To observe the appearance of the AP-site in the substrate, we added EDTA to the reaction mix to a final concentration of 4 mM to inhibit divalent cation-dependent reactions that might cleave the AP-site, and NaBH4, which stabilizes the AP site as it forms but does not inhibit UDG or interfere with subsequent endonuclease cleavage (21, 24). In order to confirm that the uracil-removal was due to UDG, we examined to what degree Ugi (25) was able to inhibit the reaction and also the degree of enhancement on a single stranded substrate, which is a characteristic of UDG. We then subjected the purified products to cleavage with recombinant human AP endonuclease, which does not cleave the substrate unless an AP site is present. Figure 3 illustrates that extracts from all stages of development were able to remove uracil from the 45-mer and that the activity was entirely inhibited by Ugi. Uracil removal in embryos and eggs was ~60% more efficient on a single-stranded substrate than on a ds substrate, which is characteristic of UDG (26). Extracts from adult fish, however, were not more active on a single-stranded substrate, which could imply the presence of additional Ugi-sensitive glycosylases. Alternatively, there may exist additional nucleases active on a single-stranded oligonucleotide in extracts from adult fish that are not present in eggs and early embryos. Since the rate of removal of uracil from a ds substrate in egg extracts was about half that in adult extracts, it was likely that part of the diminished activity in BER steps 1 and 2 seen in egg extracts was due to diminished processing of substrate at Step 1 in egg extracts.

Figure 3
Uracil removal is performed by uracil DNA glycosylase, as shown by complete sensitivity to the UDG inhibitor Ugi

Extracts from eggs, embryos at different stages of early development and adults cleave an AP-site efficiently

ZAP1 is present in eggs and throughout development (16). To avoid complications that might arise from reactions preceding the cleavage of the AP-site, we used a substrate with the same reduced AP site employed for examining the kinetics of human AP endonuclease (19, 20). This substrate allowed us to answer questions about the efficiency of AP-site cleavage by extracts from eggs, embryos and adults. The time course was linear for ~ 2 min when 2 μg protein from either adult or egg extracts was added to the mix, as shown in Figure 4A. Using the 1 min time interval, all extracts were able to cleave an AP-site containing substrate (Figure 4B). However, extracts from unfertilized eggs had about 1/4 the activity of extracts from adults, while embryos less than 72 hours after fertilization (hpf) had about half the activity of adult extracts. We showed earlier that the protein level of ZAP1 was constant throughout early development even as cellular proliferation continued (16). Nevertheless, the diminution in efficiency of the first three steps in the BER pathway in extracts from eggs as opposed to adults was due to diminished entry into the pathway via UDG and less efficient AP endonuclease activity in extracts from the former.

Figure 4
AP site cleavage is inefficient in eggs and less efficient in pre-hatching embryos than at later times

Knockdown of ZAP1 levels does not alter BER in early embryogenesis

Microinjection of MO into early embryos is a standard way of knocking down protein levels in zebrafish (17). The degree of knockdown varies with the amount of microinjected MO. In this series of experiments we knocked down ZAP1 levels by 74% or 54%, as shown by Western blot analysis (data not shown) and examined both the ability to cleave an AP-site (Figure 4, 3.5* and 6**) and the time course of the first three steps in BER (Figure 5). Extracts were prepared from control and full knockdown embryos just after the MBT at 3.5 hpf (3.5*); extracts were also prepared from control and hypomorphic embryos at 6.5 hpf (6**) several hours after the MBT. Despite loss of much of ZAP1 protein in full knockdowns and somewhat less in hypomorphs, we saw no difference in the first three steps of BER in comparison with controls nor was there any change in the ability to cleave an AP site.

Figure 5
Time dependent processing of 45-mer substrate is not altered by diminution of ZAP1

Another AP endonuclease is expressed in very early stage embryos

Since suppression of translation of ZAP1 did not result in complete loss of the protein, there may still have been sufficient ZAP1 in hypomorphs and full knockdown embryos to provide endonuclease activity for BER. Nevertheless, the possibility remained that another AP endonuclease might be expressed during development. A potential candidate is AP endonuclease 2, whose enzymatic activity in humans is 1% that of its paralogue (27, 28) but which is involved in processing of AP sites during immunoglobulin class switching (29, 30). Therefore, we chose to examine whether AP endonuclease 2 might be expressed duing early embryological developmental stages. Using gapdh as the standard of normalization, qRT-PCR revealed that both AP endonucleasel and 2 were expressed in early stage embryos at a level 13 and 20-fold that of adults respectively (Figure 6). Therefore, cleavage of an AP-site by extracts of unfertilized eggs and early embryos could be the result of both proteins.

Figure 6
AP endonuclease 2 may serve a redundant function for AP endonuclease 1 during very early development

Before hatching at 72 hpf, replicative polymerases predominate in BER

Po1-β is the preferred polymerase for insertion of a single nucleotide during BER of cultured cells and tissues from adult animals (12, 13, 18). During long patch repair, po1-β, the replicative polymerases α, and δ/ε together with PCNA or one of several bypass polymerases may participate (31-35). The presence in the reaction mix of the single nucleotide dCTP limited the reaction to short patch repair, whereas the presence of all four dNTPs enabled progression of the alternative long patch pathway. In order to examine the preferred polymerases during short patch (single nucleotide insertion) and long patch (insertion of 2-6 nucleotides) BER during development, we examined whether aphidicolin or dideoxyCTP could inhibit nucleotide insertion. Inhibition by aphidicolin is diagnostic for participation of the replicative polymerases α, δ and ε (36), while inhibition by ddCTP is considered diagnostic for po1-β (18) (Figures 7 and and8).8). Because po1-β has no proofreading ability, it readily inserts but cannot remove ddCTP, which then prevents further chain elongation or ligation.

Figure 7
The bulk of short patch repair before hatching (72 hpf) is mediated by replicative polymerases
Figure 8
Unlike synthesis in adult extract, both short patch and long patch insertion in egg extracts is mediated by replicative polymerases

In extracts from egg and early stages of development single nucleotide insertion was far more sensitive to inhibition by aphidocolin than in extracts from 24 hpf or older embryos and adults (Figure 7). Neither the ability to insert a single nucleotide nor the sensitivity to aphidicolin was altered by apex1 knockdown. By 24 hpf, aphidicolin's ability to reduce insertion had decreased. At this time the drug decreased incorporation by only 33% from control values, indicating a shift towards insertion by a polymerase resistant to aphidicolin. After hatching (3 dpf) the rate of insertion even exceeded levels in extracts from adult fish. Finally, in extracts from adult fish aphidicolin was able to decrease single nucleotide insertion by only ~15%. This last level was consistent with aphidicolin's effect on BER (31, 37, 38) and with the participation of pol-β in both long and short patch repair (39) from a variety of adult tissues and from many cultured cell lines.

Comparison of repair intermediates by extracts obtained from adults and unfertilized eggs in the presence of all four dNTPs using a 5′-end labeled substrate enabled us to confirm and extend the results obtained in the presence of dCTP alone (Figure 8). Despite the presence of exonuclease activity, the incubation time was continued to 60 min in order to better measure long patch repair. Figure 7 illustrates the involvement of replicative polymerases in both short and long patch repair as shown by sensitivity to aphidicolin in extracts from unfertilized eggs but not in extracts from adult fish. Figure 7 also shows the relative insensitivity to dideoxyCTP of BER by egg extracts, as shown by a lower 21-mer to long patch product ratio. These data confirm the surprising lack of involvement of pol-β in BER performed by extracts from eggs.

Pol-β protein does not appear until after the midblastula transition

The use of aphidicolin-dependent polymerases in BER in the unfertilized egg and very early embryogenesis could be due to replicative polymerases outcompeting pol-β for the cleaved AP site or to a lack of pol-β. Therefore, we performed Western blot analysis on embryonic extracts obtained from unfertilized eggs and from embryos at different stages of development. Figure 9 represents data showing that pol-β was not detectable in unfertilized eggs and very early stage embryos. However, the amount of pol-β relative to β-actin reached adult levels by 13 hpf.

Figure 9
Pol-β is not detected in unfertilized eggs and very early stage embryos

Discussion

Here we have presented the first data on the first three steps of the BER pathway in early zebrafish embryogenesis. Cell-free extracts from unfertilized eggs and early stage embryos as well as from adult fish are capable of recognizing and removing uracil in a U/G mispair by uracil DNA glycosylase, cleaving the resulting AP-site, and inserting the correct cytosine residue either during short patch or long patch repair. The overall pathway is less efficient in extracts from unfertilized eggs and early stage embryos. At the glycosylase step and the endonuclease step, extracts from unfertilized eggs and very early stage embryos have 1/4-1/2 the enzymatic activity of UDG and ZAP1 found in extracts from adult fish, which contributes to the lower rate of deoxynucleotide insertion in short patch synthesis. The difference lessens at ~3 dpf when hatching has occurred. This observation is consistent with the role that BER plays in repairing oxidatively damaged DNA. After hatching, the embryo is no longer protected by the chorionic membrane and must be prepared for exposure to whatever conditions it encounters in the environment.

Once a zebrafish egg is fertilized, it undergoes ten rapid division cycles to form the blastula in less than 3 hours (17). The preponderance of replicative polymerases in both short and long patch repair in eggs and very early stage embryos can be explained by the lack of pol-β and large amounts of replicative polymerases stored in preparation for the rapid cell division that follows fertilization. At the MBT cell division slows, zygotic transcription begins, spatial differentiation arises and the cells become motile. The egg stockpiles all the components necessary for the initial rapid cell division including DNA polymerases and accessory proteins, dNTPs and the mitochondrial systems to regenerate the required ATP. However, ATP generation is inevitably accompanied by production of ROS with the concomitant damage to DNA (1-3). Therefore, the BER pathway is an important component in early embryogenesis.

The reduction in ZAP1 levels by 74% in full knockdown embryos leads to little, if any, reduction in BER activity. Although the remaining protein might be sufficient to provide endonuclease cleavage, there is still the possibility that a backup activity is present in eggs and early stage embryos. To our surprise, there is 20 times the apex2 message in early stage embryos as in adults. In fact, mRNA of both apex genes is expressed at higher levels in the early stage embryos in comparison to adult fish. Thus, AP endonuclease 2 with less than 1% the activity of AP endonuclease 1 on a molar basis in vitro may play an important role in early embryological development. The former has recently been shown to participate in processing of AP sites during class switching in lymphocyte maturation (29).

Finally, although pol-β message is expressed in early development (XJ Yang, Unpublished results), pol-β protein appears in detectable amounts only after 6.5 hpf. The aphidicolin-sensitive replicative polymerase activity that provide nucleotide insertion during BER in unfertilized eggs and early stage embryos gradually diminishes to levels found in adult tissues. While the involvement of replicative polymerases in BER could be enhanced or promoted by the presence of a factor that blocks pol-β access much like the APC protein (40), the tight coordination inherent in single nucleotide BER through the interaction of pol-β, DNA and AP endonuclease seen in adult tissues (41, 42) is abrogated by the lack of pol-β in very early embryonic development.

In short, BER activity in eggs and early stage zebrafish embryos has several unexpected features consistent with rapid cellular proliferation before differentiation begins, most notably the use of aphidicolin-sensitive replicative polymerases in both short and long patch repair and the presence of standby AP endonuclease activity. Once differentiation is initiated, the pathway gradually evolves to the one found in cultured cells and adult tissues. By the time the embryo hatches from the chorionic membrane and is fully exposed to environmental conditions, the transformation is complete.

Acknowledgments

The authors are grateful to Dr. Samuel Bennett for the gift of the uracil DNA glycosylase inhibitor, Ugi.

Abbreviations

AP
abasic site
BER
base excision repair
dpf
days post fertilization
ds
double strand
hpf
hours post fertilization
MBT
midblastula transition
MO
morpholino oligonucleotide
pol-β
DNA polymerase-β
qRTPCR
quantitative real-time polymerase chain reaction
ROS
reactive oxygen species
TS-MO
morpholino oligonucleotide directed against the translation start site
UDG
eukaryotic uracil DNA glycosylase
Ung
prokaryotic uracil DNA glycosylase
ZAP1
zebrafish AP endonuclease 1 protein

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