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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nat Immunol. Author manuscript; available in PMC 2010 March 1.
Published in final edited form as:
Nat Immunol. 2009 September; 10(9): 1000–1007.
Published online 2009 July 26. doi: 10.1038/ni.1774

Fig. 2

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CD4+ YFP+ Foxp3 cells have a non-Treg surface phenotype

(A) Lymph node and spleen CD4+ GFP+ T cells were FACS purified from Foxp3-GFP-Cre mice and CD4+ YFP+ T cells sort-purified from Foxp3-GFP-Crex R26-YFPmice. Purified populations were analyzed for CD127, CD25, and GITR and Foxp3 by intracellular staining. (B) As in (A), analysis of FR4, CTLA-4 and CD103 expression and Foxp3 by intracellular staining. Histograms show YFP Tconv (thick line), YFP+ Foxp3+ Treg (thin line), and YFP+ Foxp3 (filled histogram). Representative plots from 6–9 week old mice of 3 experiments.

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