CD4+ YFP+ Foxp3− cells have a non-Treg surface phenotype
(A) Lymph node and spleen CD4+ GFP+ T cells were FACS purified from Foxp3-GFP-Cre mice and CD4+ YFP+ T cells sort-purified from Foxp3-GFP-Crex R26-YFPmice. Purified populations were analyzed for CD127, CD25, and GITR and Foxp3 by intracellular staining. (B) As in (A), analysis of FR4, CTLA-4 and CD103 expression and Foxp3 by intracellular staining. Histograms show YFP− Tconv (thick line), YFP+ Foxp3+ Treg (thin line), and YFP+ Foxp3− (filled histogram). Representative plots from 6–9 week old mice of 3 experiments.