AIM2 recognizes cytosolic dsDNA and forms a caspase-1 activating inflammasome with ASC

doi:10.1038/nature07725

Nature. Author manuscript; available in PMC 2009 September 26.

Published in final edited form as:

Nature. 2009 March 26; 458(7237): 514–518.

Published online 2009 January 21. doi: 10.1038/nature07725

PMCID: PMC2726264

NIHMSID: NIHMS103043

AIM2 recognizes cytosolic dsDNA and forms a caspase-1 activating inflammasome with ASC

Veit Hornung,^1,² Andrea Ablasser,^1,² Marie Charrel-Dennis,¹ Franz Bauernfeind,^1,² Gabor Horvath,¹ Daniel R. Caffrey,³ Eicke Latz,¹^* and Katherine A. Fitzgerald¹^*

¹Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USA

²Institute of Clinical Chemistry and Pharmacology, Universitätsklinikum Bonn, Germany

³Pfizer, 620 Memorial Drive, Cambridge, MA 02139, USA

Correspondence and requests for materials should be addressed to K.A.F, E.L or V.H.; Email: kate.fitzgerald/at/umassmed.edu, Email: eicke.latz/at/umassmed.edu or ; Email: veit.hornung/at/uni-bonn.de

^*Equal contributors

Author Contributions V.H conceived the research and conducted the experiments with A.A., M.C.D., F.B, G.H. D.R.C performed sequence analysis. E.L and K.A.F oversaw the whole project.

The publisher's final edited version of this article is available at Nature

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Abstract

The innate immune system senses nucleic acids via germ-line encoded pattern recognition receptors. RNA is sensed via Toll-like receptor (TLR)−3, −7 and −8 or by the RNA helicases RIG-I and MDA-5¹. Little is known about sensors for cytoplasmic DNA which trigger antiviral and/or inflammatory responses²^–⁶. The best characterized of these responses involves activation of the TANK-binding kinase (TBK1)-Interferon Regulatory Factor (IRF)-3 signaling axis to trigger transcriptional induction of IFN left angle bracket

/® genes²^,³. A second, less well-defined pathway leads to the activation of an ‘inflammasome’ which via caspase-1, controls the catalytic cleavage of the pro-forms of the cytokines IL-1β and IL-18⁶^,⁷. Here we identify the IFI20X/IFI16 (PYHIN) family member⁸, absent in melanoma 2 (AIM2), as a receptor for cytosolic DNA which regulates caspase-1. The HIN200 domain of AIM2 binds to DNA, while the PYD domain (but not that of the other PYHIN family members) associates with the adapter molecule ASC to activate both NF-κB and caspase-1. Knockdown of AIM2 abrogates caspase-1 activation in response to cytoplasmic dsDNA and the dsDNA virus, vaccinia. Collectively, these observations identify AIM2 as a novel receptor for cytoplasmic DNA, which forms an inflammasome with the ligand and ASC to activate caspase-1.

Our current understanding of the mechanisms sensing cytoplasmic DNA is limited⁹. A candidate receptor called DAI (DNA-dependent Activator of IFN-regulatory factors) has been implicated in the DNA-induced type I IFN pathway⁴. The NLR family member, NLRP3 has also been shown to activate caspase-1 in response to internalized adenoviral DNA⁶. Caspase-1 activation in response to transfected bacterial, viral, mammalian or synthetic DNA however, does not involve NLRP3, although the adapter molecule ASC is required⁶^,⁷.

We hypothesized that an upstream activator of this dsDNA activated ASC pathway would contain a pyrin domain (PYD) for homotypic interaction with ASC and at least one additional domain, for direct binding to DNA or for association with an upstream receptor. In addition to NLRP3¹⁰, NLRP6¹¹ and NLRP12¹² have previously been shown to associate with ASC. Although ASC-deficient macrophages failed to activate caspase-1 and trigger IL-1β release in response to poly(dA-dT) · poly(dA-dT) [hereafter referred to as poly(dA-dT)])², macrophages lacking NLRP3, −6 and −12 responded normally (Fig. 1a). Surprisingly, we found that macrophages lacking ASC had higher levels of IFNβ and IL-6 in response to poly(dA-dT), which was not observed in cells lacking NLRP3, the IL1R or to a lesser extent caspase-1 (Supplementary Fig. 1a–d). Poly(dA-dT) induced cell death also occurred in an ASC-dependent manner (Supplementary Fig. 1e–f). We speculate, that the increased cytokine production in ASC-deficient cells relates to their resistance to poly(dA-dT) induced cell death. In addition to poly(dA-dT), dsDNA from natural sources activated caspase-1 cleavage (Supplementary Fig. 2a–b). In contrast, a small immunostimulatory oligonucleotide (ISD)³, long ssDNA (poly(dI)), transfected dsRNA or the ssRNA virus Sendai virus failed to trigger this response in NLRP3-deficient macrophages (Supplementary Fig. 2c).

Figure 1

poly(dA-dT)-induced inflammasome activation

Searching the PFAM database¹³ we identified several PYD-domain containing proteins, which also contained a HIN200 domain, previously shown to bind DNA¹⁴,. In humans, the HIN200 family consists of four members¹⁵; IFIX¹⁶, IFI16¹⁷, MNDA¹⁸ and AIM2¹⁹. A multiple sequence alignment of PYD domains from these proteins with PYD domains from some of the NLRs is shown (Fig. 1b). Sequence analysis of IFIX, IFI16 and MNDA predicted their nuclear localization, in contrast to AIM2, which was predicted to be cytosolic (Fig. 1c, lower panel). Consistent with these predictions, fluorescent protein chimeras of IFIX, IFI16 and MNDA localized to the nucleus, while AIM2 was almost exclusively cytoplasmic (Fig. 1d).

To study the possibility that these PYHIN proteins associated with ASC, we generated C-terminally tagged CFP PYD-domain fusions (which lacked the putative nuclear localization sequences identified above). Indeed, all of the PYD-CFP fusions were localized in the cytoplasm (Supplementary Fig. 3). To test whether induced clustering of the PYD-CFP fusions leads to association with ASC-YFP, we utilized a HEK293 cell line that stably expressed ASC-YFP at low enough levels to be polydispersed throughout the cytoplasm (Fig. 2a, Mock). Indeed, overexpression of the NLRP3-CFP-tagged PYD domain led to the formation of large cytosolic aggregates, which co-aggregated ASC-YFP (Fig. 2a). Notably, in most transfected cells, extensive intracellular co-localization with ASC-YFP was observed with a complete loss of cytoplasmic distribution of ASC-YFP¹⁰^,¹¹^,²⁰. Of all the PYHIN-PYD proteins tested, only AIM2-PYD led to complex formation with ASC (Fig. 2a, b and Supplementary Fig. 4). Similar results were obtained with full-length AIM2-CFP but not full-length IFIX, IFI16 or MNDA, which were all localized to the nucleus (Fig. 2a, b and Supplementary Fig. 5a, b). Additionally, only AIM2-PYD and NLRP3-PYD were found to bind HA-tagged ASC in co-immunoprecipitation studies (Fig. 2c). Finally, endogenous ASC associated with endogenous AIM2, but not IFI16 in primed THP-1 cells (Fig. 2d).

Figure 2

AIM2 interacts with ASC

To examine the functional relevance of AIM2-ASC complex formation, we examined NF-κB reporter gene activity in cells overexpressing the PYD-PYHIN proteins in the presence of ASC. Only NLRP3-PYD and AIM2-PYD led to potent NF-κB activation (Fig. 2e). The effect of full length AIM2 was even more dramatic (Fig. 2e, bottom panel). The full-length versions of IFIX, IFI16 and MNDA failed to activate NF-κB (Supplementary Fig. 5c). ASC was absolutely required, since no substantial NF-κB reporter activity was observed in cells not transfected with ASC. No substantial activation of the IFNβ promoter reporter gene was observed with any of the PYHIN family members (Supplementary Fig. 6).

We next examined whether the AIM2-ASC complex could lead to the formation of a functional inflammasome complex and caspase-1-dependent maturation of pro-IL-1β. We employed a transient transfection assay overexpressing the respective proteins of interest in the presence of ASC, caspase-1 and flag-tagged pro-IL-1β in 293T cells and monitored the cleavage of pro-IL-1β by immunoblotting. Among the PYD proteins tested, only that of NLRP3-PYD and AIM2-PYD induced maturation of pro-IL-1β, when ASC and caspase-1 were co-expressed (Fig. 3a). Full-length AIM2 was even more potent than AIM2-PYD (Fig. 3a, lower panel). Neither the PYD domain nor the full-length versions of IFIX, IFI16 or MNDA induced IL-1β cleavage (Supplementary Fig. 7).

Figure 3

AIM2 is required for poly(dA-dT)- and vaccinia virus-triggered inflammasome activation

To study the role of AIM2 in cells with a functional poly(dA-dT)-triggered or dsDNA virus induced inflammasome complex, we used lentiviruses encoding shRNAs to knock down AIM2 in immortalized murine macrophage cell lines (B6-MCLs or N3-KO-MCLs)⁷. AIM2 was expressed constitutively in both primary macrophages and in B6-MCLs and was further induced by poly(dA-dT) or Sendai virus (Supplementary Fig. 8). Three different shRNAs were tested, of which two (shRNA AIM2 #2 and AIM2 #3) resulted in a strong reduction of AIM2 expression (Fig. 3b). Knocking down AIM2, but not an unrelated gene, resulted in a strong attenuation of poly(dA-dT)-mediated IL-1β release (Fig. 3c) and caspase-1 cleavage (Fig. 3d). Targeting AIM2 in THP1 cells using siRNA corroborated these findings (Supplementary Fig. 8d and e). Moreover and consistent with what we had seen in ASC-deficient macrophages (Supplementary Fig. 1), knocking down AIM2 resulted in a marked enhancement of poly(dA-dT)-mediated type I IFN induction (Supplementary Fig. 8b). This effect was specific since the IFNβ response to Sendai virus was unaffected (Supplementary Fig. 8c). In addition, and in agreement with the results obtained in ASC-deficient macrophages, macrophages that were targeted with AIM2 shRNAs were resistant to poly(dA-dT) triggered cell death (Fig. 3e). We also examined the role of AIM2 in the recognition of the dsDNA virus vaccinia. Similar to what we had observed with transfected poly(dA-dT), vaccinia virus-induced caspase-1 cleavage occurred in an ASC-dependent but NLRP3-independent manner (Fig. 3f). This effect was also dependent on AIM2, since shRNA-mediated knock down of AIM2 impaired vaccinia virus induced caspase-1 cleavage but not that induced by anthrax lethal toxin (Fig. 3g). Moreover, knock down of a control protein did not affect caspase-1 cleavage after vaccinia virus infection. Vaccinia virus-triggered cell death was also strongly reduced in AIM2 shRNA targeted macrophages, but not in control macrophages (Fig. 3h). Altogether, these results indicated that AIM2 controlled inflammasome activation and cell death in response to dsDNA and the dsDNA virus vaccinia.

To examine if AIM2 could be involved in the recognition of dsDNA directly, we generated fluorescein-labeled poly(dA-dT), (FITC-dsDNA) and co-transfected FITC-dsDNA together with CFP-tagged versions of full-length AIM2, AIM2-HIN domain, AIM2-PYD domain or full-length NLRP3. While cells expressing NLRP3 or AIM2-PYD showed no co-localization of the respective proteins with FITC-dsDNA, full-length AIM2 and AIM2-HIN domain showed extensive co-localization with FITC-dsDNA and led to the formation of DNA/protein aggregates in the cytosol (Fig. 4a). We used single cell flow cytometry fluorescence resonance energy transfer (FRET) measurements to quantify these interactions (Fig. 4b). A dose-dependent increase in FRET between full-length AIM2 and FITC-dsDNA was seen, while AIM2-PYD did not lead to measurable FRET. Other proteins such as NLRP3 or IFI16 did not show any FRET (data not shown). Additionally, binding studies using purified AIM2, AIM2-HIN domain and AIM2-PYD domain with biotinylated poly(dA-dT) (biotin-dsDNA) revealed that AIM2 directly interacted with poly(dA-dT) with high affinity; only full-length AIM2 or the AIM2-HIN domain were able to bind biotin-dsDNA (Fig. 4c). Binding of poly(dA-dT) to AIM2 was specific, since AIM2 did not bind biotin-LPS, which bound to soluble CD14 under similar assay conditions (Fig. 4c).

Figure 4

AIM2 binds dsDNA via its HIN domain

Collectively, these data identify AIM2 as a receptor for cytosolic dsDNA, which forms a novel inflammasome complex with ASC to activate caspase-1-mediated processing of IL-1β. Our data also indicate that the activation of the AIM2 inflammasome is important in innate immunity to vaccinia virus. Since bacterial pathogens such as Francisella tularensis ²¹ and aberrant host DNA in pathological autoimmunity²² also trigger the IL1β pathway, it will also be important to define the role of AIM2 in these responses. Further characterization of the AIM2 inflammasome as a sensor of microbial, as well as host DNA therefore, may enable the rational design of new therapies and treatments for infectious as well as autoimmune diseases.

Methods Summary

Reagents

All cDNAs were cloned by PCR from cDNA into pEFBOS-C-term-CFP and subcloned into pEFBOS-C-term-FLAG/HIS. Biotinylated and FITC-labeled poly(dA-dT) were made by adding Biotin-dUTP or FITC-dUTP (Fermentas) in a molar ratio of 1:8 to dTTP in the enzymatic synthesis of poly(dA-dT) as described²³. Vaccinia virus (Western Reserve strain) was from K. Rock (UMASS, Worcester, MA). Anti-human AIM2 antibody (3B10) was from R. Johnstone (The Peter MacCallum Cancer Institute, Victoria, Australia).

Mice

Cell culture and stimulation

Bone marrow derived macrophages were stimulated as indicated. Poly(dA-dT) DNA and all other DNAs were transfected using Lipofectamine 2000 at a concentration of 1 µg/ml.

ELISA

Cell culture supernatants were assayed for IL-1β using ELISA kits from BD Biosciences (Franklin Lakes, NJ).

Confocal microscopy

Confocal microscopy was performed on a Leica SP2 AOBS confocal laser scanning microscope.

Flow cytometry Fluorescence Resonance Energy Transfer (FRET)

FRET efficiencies were calculated on a cell-by-cell basis²⁵ and histograms were plotted with GraphPad Prism 5.01 (GraphPad Software).

Immunoblot analysis

Immunoblot analysis was conducted as previously described⁷.

Quantitative real-time PCR

Quantitative RT-PCR analysis performed as previously described²⁶.

shRNA mediated silencing

Lentiviral shRNAs targeting AIM2 were obtained from OpenBiosystems and shRNA silencing carried out as described (http://www.broad.mit.edu/genome_bio/trc/publicProtocols.html).

AlphaScreen Assay

The AlphaScreen (amplified luminescent-proximity homogeneous assay) was set up as an association assay and read with the Envision HT microplate reader (Perkin Elmer).

Reporter assays

Reporter assays for NF-κB or IFN® luciferase reporters were carried out as previously described²⁶.

ONLINE METHODS

Plasmid constructs

Full length human AIM2 (1–356; based on BC010940.1), AIM2-PYD (1–83), AIM2-HIN (148–356), IFIX (1–461; based on NM_198929), IFIX-PYD (1–84), IFI16 (1–729; based on NM_005531), IFI16-PYD (1–84), MNDA (1–407), MNDA-PYD (1–84; based on NM_002432) and NLRP3-PYD (1–87; based on NM_004895.3) were cloned by PCR from cDNA into pEFBOS-C-term-CFP using PCR-generated XhoI and BamHI or BglII restriction sites. AIM2 full length, AIM2-PYD and AIM2-HIN were subcloned into pEFBOS-C-term-FLAG/HIS using XhoI and BamHI. Murine pro-IL-1β (1–269) was obtained by PCR from cDNA and fused into pEFBOS-C-term-GLuc/FLAG using XhoI and BglII/BamHI. Expression plasmids (pCI) encoding human Caspase-1 and ASC-HA were from Millenium Pharmaceuticals (Cambridge, MA).

Reagents

ATP, LPS, poly(dA-dT), were from Sigma-Aldrich. A555-conjugated choleratoxin B was obtained from Molecular Probes, Invitrogen. DRAQ5 was from Biostatus. Biotinylated and FITC-labeled poly(dA-dT) were made by adding Biotin-dUTP or FITC-dUTP (Fermentas) in a molar ratio of 1:8 to dTTP in the enzymatic synthesis of poly(dA-dT) as described²³. Anthrax lethal toxin was from List Biologicals (5 µg·ml−1 PA and 5 µg·ml−1 LF). Anti-human AIM2 antibody (3B10) was from R. Johnstone (The Peter MacCallum Cancer Institute, Victoria, Australia).

Mice

NLRP3 ^−/− and Pycard ^−/− mice were as previously described²⁴. Both strains, as well as NLRP6^−/− and NLRP12^−/− mice were from Millennium Pharmaceuticals (Cambridge, MA) and Caspase-1-deficient mice were from R. Flavell (Yale University, New Haven, CT). C57BL/6 mice, 129/Sv mice, C57/Bl6 × 129 F1 mice, IL-1R-deficient (Il1r1 ^−/−) were from Jackson Laboratories (Bar Harbor, ME). All mouse strains were bred and maintained under specific pathogen-free conditions in the animal facilities at the University of Massachusetts Medical School.

Sequence Analysis

Pyrin domain containing sequences were retrieved from UniProt after identifying them in SMART. A multiple sequence alignment of selected pyrin domains was generated using MUSCLE²⁷. Secondary structure elements from the POP1 crystal structure (PDB code: 2HM2) were mapped to the multiple sequence alignment in PFAAT²⁸. Prediction of nuclear targeting sequences and subcellular localization was done using PSORTII (http://psort.ims.u-tokyo.ac.jp/).

Cell culture and stimulation

Bone marrow derived macrophages were generated and cultured in DMEM medium as described²⁶. THP1 cells and macrophage cell lines were cultured as previously described. One day prior to stimulation, THP1 cells were differentiated using 0.5 µM PMA⁷. ATP (5 mM) was added one hour prior to harvesting supernatants. Poly(dA-dT) DNA and all other DNAs were transfected using Lipofectamine 2000 at a concentration of 1 µg/ml according to the manufacturer’s instructions. Expression plasmids were transfected into 293T cells using GeneJuice (Novagen). Vaccinia virus (WR strain) was used for infection at an MOI of 5 if not indicated otherwise.

ELISA

Cell culture supernatants were assayed for IL-1β by ELISA (BD Biosciences, Franklin Lakes, NJ). To measure intracellular IL-1β, cells were washed and subjected to three freeze thaw cycles in assay diluent.

Confocal microscopy

Confocal microscopy was performed on a Leica SP2 AOBS confocal laser scanning microscope. Separation of CFP and YFP was performed using sequential scanning and 405 and 514 nm excitation.

Flow cytometry Fluorescence Resonance Energy Transfer (FRET)

Three fluorescent intensities were measured: I1 – direct excitation and emission of donor, I2 – indirect excitation and direct emission of acceptor and I3 – direct excitation and emission of acceptor. After full correction for spectral bleed-through and cross excitation, FRET efficiency was calculated on a cell-by-cell basis²⁵ and then FRET efficiency histograms plotted with GraphPad Prism 5.01 (GraphPad Software).

Immunoblot analysis

Immunoblotting was conducted as previously described⁷ using anti murine caspase-1 p10 (sc-514, Santa Cruz Biotechnology), anti FLAG (M2, Sigma), anti HA (Roche Applied Science), anti CFP (Santa Cruz Biotechnology), anti ASC (Alexis, AL177), anti IFI16 (sc-8023, Santa Cruz Biotechnology) or anti AIM2 (3B10 mouse IgG1²⁹).

Co-immunoprecipitation assays

293T cells (24 wells) were transfected with 1550 ng of the PYD-CFP expression plasmids and 50 ng of ASC-HA. 24h later, cell lysates were cleared by centrifugation (20.000 g, 30 min) and subsequently incubated with anti-HA agarose beads for 2h at 4°C. Differentiated THP1 cells were primed with Sendai virus (300 HAU/ml) overnight and subsequently lysed in high salt lysis buffer (250 mM NaCl, 10 mM Tris HCl pH 7.4, 1% CHAPS, protease inhibitor cocktail). Lysates were cleared by centrifugation, salt concentration adjusted to 125 mM NaCl and ASC immunopreciptated using polyclonal rabbit anti-ASC. After 6 washes in both cases beads were boiled with Laemmli buffer for immunoblot analysis.

Quantitative real-time PCR

Quantitative RT-PCR analysis was performed as described²⁶. Primer sequences for murine HPRT1, IFNβ, AIM2 and IL-6 are available upon request.

shRNA mediated silencing

The lentiviral shRNA expression plasmids were from OpenBiosystems (Huntsville, AL). The shRNAs targeting AIM2 are TRCN0000096104 (#1), TRCN0000096105 (#2), TRCN0000096106 (#3). The control shRNA is directed against murine IFIH1 (TRCN0000103648) and was confirmed not to have any impact on NLRP3 or AIM2 expression. The production of viral particles and transduction of target cells was conducted as described on http://www.broad.mit.edu/genome_bio/trc/publicProtocols.html.

AlphaScreen Assay

The AlphaScreen was set up as an association assay. Proteins were transiently expressed in 293T cells and purified using FLAG beads (Sigma) binding to the C-terminal FLAG/HIS tag. The protein of interest was incubated at a concentration of 100 nM with biotinylated ligand at the indicated concentration in PBS, 0.1% BSA, and 0.01% Tween 20 for 60 min. Subsequently, Nickel chelate acceptor beads (binding to the HIS tag) and streptavidin-coated donor beads were added. After 30 min incubation at 25°C in the dark, samples were read in proxiplates with the Envision HT microplate reader (all Perkin Elmer). Data were analyzed by GraphPad Prism.

Reporter assays

All reporter gene assays were conducted as described²⁶.

siRNA transfection

Differentiated THP1 cells were plated at 2.5 × 10E4 cells/ in 96-well plates and transfected with siRNA targeting human AIM2 (sense strand: 5’-CCCGAAGATCAACACGCTTCA-3’), human ASC (sense strand: 5’-CGGGAAGGTCCTGACGGATGA-3’) or human TLR8 (5’-GGGAGUUACUGCUUGAAG A-3’) using 275 ng siRNA and 0.75 µl Lipofectamine 2000. 48h after transfection cells were stimulated as indicated.

Cell viability assay

To quantify cell viability, macrophages (1 × 10E5 cells/ in 96-well plates) were treated as described. After 24h, cells were washed with PBS and incubated in PBS with 5 µM calcein AM (Invitrogen) for 30 min at 37°C. The number of viable cells was assessed by counting fluorescent cells in two independent visual fields (20x) using ImageJ or by determining the overall fluorescence intensity using an Envision HT microplate reader.

Supplementary Material

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Acknowledgements

The authors would like to thank Anna Cerny for animal husbandry and genotyping and R. Johnstone (The Peter MacCallum Cancer Institute, Victoria, Australia) for the anti-AIM2 antibody. V.H. is supported by a fellowship from the DFG (Ho2783/2–1), E.L. and K.A.F. are supported by grants from the NIH (AI-065483 (to E.L.) and AI-067497 (to K.A.F.).

Footnotes

Author Information: Reprints and permissions information is available at npg.nature.com/reprintsandpermissions.

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