Evaluation of LLC and B16 tumors in TEM8+/+ and TEM8−/− mice. A, Tumor growth following s.c. tumor cell injection. n = 6–9 for LLC; n = 12–13 for B16. Points, means; bars, SE; *, P < 0.005; **, P < 0.002 (student’s t test). B, Vessel density in B16 tumors. Immunofluorescence staining of ECs (Meca-32, red) and pericytes pericytes (Desmin, green). Bar, 100µm. C, The number of pericytes in B16 tumors. Arrowheads, pericyte nuclei; Bar, 10µm. D, qPCR analysis of TEM8 and CMG2 expression in B16 or LLC tumor cells in culture, or purified tumor ECs (T-ECs). Error bars represent SD derived from biological replicates (separate flasks of cultured cells or independently isolated T-EC samples).