Physiological angiogenesis is unaltered in TEM8−/− mice. Vessel structure at P10.5 in the developing retina (A), the rate of wound closure (B), the number of vessels in wounds (C) and vessel structure in the uterus (D) appeared similar between knockout and wildtype mice. D, In the knockouts a decreased nuclear density (DAPI, blue) corresponding to areas with excessive ECM deposits (see Fig. 2) was frequently observed. CD31-positive vessels (green); NG2-positive pericytes (red). Bar, 20µm.