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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Cancer Metastasis Rev. Author manuscript; available in PMC 2010 June 1.
Published in final edited form as:
PMCID: PMC2720096

Tissue architecture and function: dynamic reciprocity via extra- and intra-cellular matrices


Mammary gland development, functional differentiation, and homeostasis are orchestrated and sustained by a balance of biochemical and biophysical cues from the organ’s microenvironment. The three-dimensional microenvironment of the mammary gland, predominantly ‘encoded’ by a collaboration between the extracellular matrix (ECM), hormones, and growth factors, sends signals from ECM receptors through the cytoskeletal intracellular matrix to nuclear and chromatin structures resulting in gene expression; the ECM in turn is regulated and remodeled by signals from the nucleus. In this chapter, we discuss how coordinated ECM deposition and remodeling is necessary for mammary gland development, how the ECM provides structural and biochemical cues necessary for tissue-specific function, and the role of the cytoskeleton in mediating the extra—to intracellular dialogue occurring between the nucleus and the microenvironment. When operating normally, the cytoskeletal-mediated dynamic and reciprocal integration of tissue architecture and function directs mammary gland development, tissue polarity, and ultimately, tissue-specific gene expression. Cancer occurs when these dynamic inter-actions go awry for an extended time.

Keywords: Acinar morphogenesis, Chromatin organization, Cytoskeleton, Extracellular matrix, Mammary-specific function, Microenvironment, Tissue architecture

1 Introduction

Tissue architecture is critical for cell homeostasis and tissue-specific functions [1]. Tissue microenvironments, which we define as the biochemical and biophysical cues a cell receives from the extracellular matrix (ECM), neighboring cells, the immune system and soluble factors (growth factors, hormones, and cytokines), plays an important role in regulating tissue structure and functions. Whereas the soluble factors have been know for decades to regulate tissue architecture and homeostasis, the insoluble ECM, neglected for much too long as a signaling entity [2], is gaining attention as another important regulator. Here we focus our discussion on the mechanism by which the ECM maintains tissue architecture and functions.

Epithelial cells within the organs are usually surrounded by a basement membrane (BM), a specialized form of ECM comprised largely of laminins, type IV collagen, entactin/nidogen, proteoglycans, and other glycoproteins. Cells interact with ECM molecules by specific receptors, of which integrins are the best understood (reviewed in [3]). Other ECM receptors include dystroglycans, syndecans, CD44 and Rhamm [46]. The engagement of ECM receptors induces a cascade of both physical and biochemical signals which transmit from the cell membrane to the nucleus (Fig. 1), accompanied by changes in cell and tissue morphology and architecture. These alterations involve dramatic reorganization of both the cytoskeleton and chromatin structures, leading to changes in cellular and tissue architecture and gene expression which in turn affect the microenvironment. This dynamic and reciprocal dialogue between the cells and their microenvironment acts as a circuitry by which tissue architecture and function become integrated. The wiring of this circuitry includes a major signaling axis that transmits through, and is regulated by, the cytoskeleton (Fig. 1).

Fig. 1
A scheme showing the principle of dynamic reciprocity between cells and their extracellular microenvironment. Cell-cell and cell-ECM interactions induce cascades of both physical and biochemical signals which transmit from the cell membrane to the nucleus ...

In this chapter, we discuss the following issues pertinent to the ECM-cytoskeleton-nucleus signaling axis: how coordinated ECM deposition and remodeling may be necessary for tissue morphogenesis during development; how the ECM acts as an organizing unit by which tissue architecture, polarity, and specificity are maintained; and lastly, how the cytoskeleton acts as a conduit mediating the dynamic and reciprocal signals between the ECM and the nucleus to maintain correct tissue form and function. While we focus primarily on the mammary gland as a model, the general concepts and conclusions, in principle, should be applicable to many other organs. Understanding how the cytoskeleton integrates tissue architecture with function will help also to more clearly delineate how this balance becomes perturbed and eventually exploited by tumor cells during malignancy.

2 Coordinated ECM remodeling during tissue morphogenesis

The tissue microenvironment undergoes extensive remodeling during morphogenesis, including changes in the deposition, degradation, and structural organization of ECM components. This remodeling of the ECM within a changing microenvironment provides morphogenic cues to control cell survival, proliferation, migration, polarization, and differentiation [1, 7]. Since the normal mammary gland function is regulated by repeated cycles of branching, alveogenesis, lactation, and involution, where the ECM undergoes constant assembly and degradation [811], the mammary gland provides an elegant and versatile model by which to investigate how the ECM remodeling contributes to tissue morphogenesis and functional differentiation.

During all stages of mammary gland development, the expression of ECM proteins and remodeling enzymes is tightly regulated both temporally and spatially. In situ hybridization and immunofluorescence experiments demonstrate that expression and deposition of collagen I, collagen IV, and laminin-γ2 chain correlates with mammary epithelial cell proliferation and stromal invasion during puberty and pregnancy [8]. Consistent with its roles in ductal elongation, fibrillar collagen I is predominantly localized along mammary ducts, whereas collagen IV and laminins are concentrated near endbuds and around alveoli, suggesting these ECM components contribute to normal alveologenesis and functional differentiation [8]. Hyaluronan, a non-sulfated glycosaminoglycan, is localized at the tip of terminal end buds and is mainly produced by ‘cap cells’ [12, 13], which may contribute to cell proliferation and migration. Interestingly, the expression of α2β1-integrin, the receptor of collagen I, collagen IV, and laminins, correlates with levels of ligands during mammary gland development [8]. Conditional deletion of β1-integrin from basal cells abolishes the regenerative potential of epithelial cells and impairs ductal branching and lobuloalveolar development at pregnancy stage [14], indicating that integrin-mediated signals from the ECM are necessary for normal tissue morphogenesis. Deposition of fibronectin (FN) increases appreciably during ductal morphogenesis, and parallels the elevated expression of the FN receptor α5β1-integrin in myoepithelial cells at this stage of development [15]. In three-dimensional (3D) cultures, FN expression decreases during acinar morphogenesis as cells polarize and form a lumen. Addition of exogenous FN increases cell proliferation and colony size, and treatment with FN blocking antibody reverse malignant phenotypes in 3D cultures, suggesting that FN coordinates epithelial cell growth during mammary gland development [16, 17]. Taken together, these studies implicate ECM components as regulators of mammary gland morphogenesis.

Remodeling the ECM microenvironment requires the activity of ECM-degrading enzymes such as matrix metalloproteinases (MMPs) [7, 18, 19]. Analogous to the deposition of ECM components, ECM remodeling proteases have distinct temporal and spatial expression patterns during mammary gland development. MMP3 is highly expressed in fibroblasts lining the mammary ducts during ductal elongation, and becomes reactivated upon mammary gland involution [2022], during which ECM degradation by MMPs coordinates the regression and reorganization of mammary structures to a state resembling that of a virgin gland. The expression pattern of MMP-2 is similar to MMP3 in the virgin gland, but MMP2 activity is reduced at terminal end buds [23]. MMP14 is expressed in both the stromal and epithelial compartments along the ducts, but is highly concentrated at terminal end buds [23]. We have found recently that MMP14 is expressed mainly in the myoepithelial cells and is crucial for branching morphogenesis [24]. MMP-dependent ECM remodeling breaks down a physical barrier which limits epithelial cell proliferation and migration, and genetic manipulation of MMP expression causes aberrant ECM remodeling which impairs epithelial morphogenesis [21, 25]. Targeted expression of constitutively-activated MMP3 in the mammary epithelium enhances branching morphogenesis and leads to precocious alveolar development in mice, accompanied with increased cell proliferation and the loss of basement membrane integrity [21, 25]. The numbers of ductal branches are significantly reduced during mid-puberty in MMP-3 null mice, while targeted deletion of MMP2 delays the invasion of mammary ducts into the fat pads during early puberty [23]. Degradation of the ECM can also generate or release cryptic fragments that act as growth stimulators or inhibitors to dictate tissue morphogenesis. Cleavage of laminin-γ2 chain with MMP14 generates an EGF-like fragment promoting epithelial cell migration and proliferation [26]; correspondingly, MMP14-deficient mice have significantly reduced processing of the γ2 chain and disrupted kidney epithelial morphogenesis [27]. Thus, MMP-dependent dynamic remodeling of ECM microenvironments generates both physical and biochemical cues necessary for tissue morphogenesis. These studies show the tight coordination of ECM deposition and turnover during development, but how ECM regulates tissue architecture and homeostasis remains unanswered.

3 ECM directs tissue polarity and function

Disruption of tissue structure usually parallels the loss of tissue-specific differentiation, suggesting that tissue architecture is intimately linked to function [28, 29]. Mammary epithelial cells that are cultured on conventional, two-dimensional (2D) plastic fail to form acinar-like structures and lose tissue-specific milk protein expression [30]. We showed that culturing mammary epithelial cells in 3D laminin-rich (lr) ECM gels (usually composed of a reconstituted lrECM derived from EHS tumors [31]) can help reconstruct the acinar structure seen in vivo and restore a number of mammary-specific functions; these processes require the orchestrated action of laminin in a gel and lactogenic hormones [32, 33]. Basal epithelial cells are tightly adherent to BM in mammary gland, which not only provides mechanical support and segregates epithelial cells from the stroma, but also directs cell polarity, proliferation, differentiation, and gene expression (for review see [29, 34]). Culturing cells in different 3D models allows delineation of the mechanisms of how the ECM microenvironment regulates tissue architecture and tissue specific function.

Normal epithelial cells in culture and in vivo have a defined apical-basal polarity, which is established by cell-ECM and cell-cell adhesions and which contributes to induction and maintenance of tissue specificity [35]. In the mammary gland, myoepithelial and luminal epithelial cells form polarized and bilayered acini, which secrete milk into the lumen of the acini during lactation. Laminin-111, a major component of BM, is secreted by myoepithelial cells, which is necessary for polarization of luminal epithelial cells [36]. Even in the absence of myoepithelial cells, luminal epithelial cells cultured in 3D lrECM establish apical-basal polarity and express milk proteins in response to lactogenic hormones [33]. The same cells cultured in an improper 3D context, such as in collagen gels lacking laminin, have reversed polarity and lose mammary-specific gene expression. Polarity and tissue-specific functions can be rescued by co-culturing luminal epithelial cells with either myoepithelial cells or with purified laminin-111 [36], confirming that the critical role of the ECM in guiding mammary specific function [37]. Accordingly, deletion of two laminin-111 receptors, β1-integrin or dystroglycan, impairs polarization of mammary epithelial cells and inhibits milk protein expression in 3D cultures [38, 39]. These studies establish the importance of correct cell polarity and physiological context in determining tissue specific function.

Why is polarity an important integrator of tissue-specificity? By definition, polarized cells have an asymmetrical distribution of proteins within the cell, including transmembrane receptors and other factors mediating tissue differentiation and function. We show that the receptor for the lactogenic hormone, prolactin, localizes to the basolateral surface of mammary epithelial cells [40]. Since prolactin is secreted into the interstitium and blood supply during pregnancy and lactation [41] and the prolactin receptor is available at the basal surface of the ducts and acini in vivo, ligand binding and subsequent signaling events occur rapidly upon prolactin release. However, in 2D culture, the cells do not express milk proteins in response to lactogenic hormone treatment because the basolaterally localized receptor is inaccessible to apically presented prolactin (Fig. 2). The asymmetric distribution of proteins within a polarized cell also sequesters inhibitory ligands from their effectors to regulate tissue-specific gene expression. An example of this is illustrated with whey acidic protein (WAP), a protein released into milk during pregnancy and lactation, and its inhibitor TGFα. Unlike β-casein, WAP is not expressed even in cells treated with lrECM and lactogenic hormones, unless cells are able to form completely polarized acini with functional tight junctions; this complete polarization separates TGFα from its receptor to allow WAP expression in epithelial cells [42, 43]. In lung epithelial cells, the EGF receptors erbB2 and erbB4 are basolaterally localized, but their ligand heregulin is secreted from the apical membrane [44]. This physical segregation limits heregulin-mediated receptor activation to periods where the epithelial integrity is disrupted such as during tissue injury and cancer progression. Cytoplasmic molecules are also asymmetrically localized in polarized epithelial cells, for example, PIP3 and PI3K, a key integrator of signaling events downstream of integrins and receptor tyrosine kinases, localize predominantly to the basal surface of polarized acinar structures in 3D cultures [45]. Perturbing the basal PI3K distribution or blocking its activity significantly inhibits milk protein expression (manuscript in preparation). Together these studies suggest that the asymmetrical localization of important signaling modulators in tissues is crucial for the activation and maintenance of mammary-specific functions.

Fig. 2
A cartoon showing that the ECM provides architectural and biochemical cues necessary for tissue-specific function. Aggregating mammary epithelial cells in suspension exposes prolactin receptor and allows transient STAT5 activation in response to prolactin ...

As discussed above, mammary epithelial cells can recapitulate some features of apical-basal polarity when cultured on 2D substrata, but the cells cannot functionally differentiate and lose their capacity to express milk proteins in response to lactogenic hormone treatment [40]. We have shown that the coordination of continuous signals from both laminin-111 and prolactin receptor are required for β-casein expression [40, 46, 47]. The discovery of an ECM-regulated transcriptional element in the promoter of a casein gene provides a mechanistic rationale for how laminin-111 may coordinate functional differentiation [48, 49]. Induction of cis-regulated DNA elements requires the activation of trans-acting transcription factors to bind at such sites; as such, the identification of ECM-response elements suggested the existence of ECM-regulated transcription factors. In fact, a number of transcription factors, including STAT5 and C/EBPβ, are modulated by ECM to regulate tissue-specific gene expression [46, 50, 51]. The Bissell laboratory previously demonstrated that establishment of tissue polarity induces the relocalization of several other factors, such as the nuclear protein NuMA, the RNA splicing factor SRm160, NFκB, TIN2, and the cell cycle regulator Rb in mammary epithelial cell line HMT-3522 S1 [5254]. Treatment of cells with a NuMA antibody leads to the disruption of NuMA foci, which in turn reverses the lrECM-induced chromatin reorganization and breaks down the endogenous BM [52]. Therefore, the integrity of ECM to nucleus dynamic reciprocity is necessary to establish normal tissue architecture and function. We hypothesized long ago that this dynamic reciprocity is coordinated along the signaling axis that is channeled through the cytoskeleton [2].

4 Cytoskeleton is a signaling axis between microenvironment and genome which sustains dynamic reciprocity

Eukaryotic cell contains three types of cytoskeletal components: microfilaments, intermediate filaments, and microtubules. Cytoskeletal proteins form macromolecular complexes at sites of cell-ECM adhesions with adaptors and signaling modulators [55], and engagement of integrins by the ECM induces reorganization of both actin and intermediate filaments (Fig. 1). Lamins, which are structural proteins comprising the nuclear envelope, are connected with cytoskeletal actin filaments through nesprin (Fig. 1), which serves to anchor the nucleus to the cytoskeleton in order to regulate nuclear localization, movement, and possibly other functions [56, 57]. As such, the cytoskeleton acts as a conduit connecting the ECM to the nucleus, allowing dynamic and reciprocal interactions between the extracellular environment and the nucleus to coordinate gene expression and tissue homeostasis.

Substantial evidence supporting the model of dynamic reciprocity includes elegant work done by Maniotis in the laboratory of Don Ingber, where it was shown that inducing mechanic strain on integrins immediately changes the shape and organization of nuclei, a mechanotransduction process which is dependent on intermediate filaments and microfilaments [58]. Culturing mammary epithelial cells in 3D lrECM reorganizes actin to have a predominantly cortical distribution similar to mammary acini in vivo, whereas actin frequently forms stress fibers when cells are cultured on plastic [59] (our unpublished data). Disruption of stress fibers with cytochalasin D (CytoD), an inhibitor of actin polymerization, leads to global histone deacetylation and cell rounding in 2D cultures, suggesting that the actin cytoskeleton mediates the ECM-induced changes to cellular architecture and chromatin organization, and correspondingly, tissue-specific gene expression [59].

ECM-dependent cytoskeletal reorganization contributes to transduction of biochemical signals which establish and maintain tissue architecture and function. In mammary cells, laminin-111 induces transmission of mechanical and biochemical signals first through dystroglycan which leads to formation of an organized BM and cell shape changes, and subsequently through β1-integrins to further alter cytoskeletal structure [37, 38, 60, 61]. These events are essential for activation of STAT5, a regulator of mammary-specific function [40]. Canonical STAT5 signal transduction is initiated by binding of prolactin to the receptor, which activates transient JAK2-mediated phosphorylation and nuclear translocation of STAT5. However, in mammary epithelial cells, the transient STAT5 activation fails to induce and maintain milk protein expression. We have shown recently that laminin-dependent biochemical signals induce a sustained activation of STAT5, and that blocking the sustained activation inhibits chromatin remodeling and milk protein expression (Fig. 2) [40]. Chromatin immunoprecipitation and photobleaching techniques have demonstrated that the association of transcription factors with cis-regulatory elements is very transient, inducing dynamic and cyclic chromatin remodeling [62, 63]. Thus, the ECM-dependent sustained activation of transcription factors is critical in order to allow the dynamic chromatin remodeling necessary for tissue homeostasis. It has been shown that integrity of the cytoskeleton is necessary for transcription factor activation and tissue-specific gene expression in mammary epithelial cells at defined stages in signal transduction pathways, as disruption of cortical actin filaments with CytoD inhibits the laminin-induced STAT5 activation and milk protein expression in 3D lrECM cultures [64]. Therefore, the cytoskeleton may provide a conduit for the transmission of transcription factor from ECM to nucleus, which in turn regulates tissue-specific gene expression.

The ECM-induced cytoskeletal rearrangement appears to occur through the Rho family of small GTPases, a diverse family of signaling molecules which are known to regulate cell migration, polarization and differentiation by influencing actin dynamics and organization [45, 6567]. Binding of laminin-111 to β1-integrin induces Rac1 activation in mammary epithelial cells, which correlates with acinar morphogenesis and activation of mammary-specific function. Overexpression of dominant-negative Rac1 disturbs acinar morphogenesis and lumen formation, and inhibits the laminin-induced STAT5 activation and milk protein expression in 3D lrECM cultures [64, 65]. Cytoskeletal-regulated signals also regulate expression of chemokines and MMPs, which in turn influence the tissue microenvironment [68]. In rabbit synovial fibroblasts, perturbing the adhesion of α5β1-integrin with a functional blocking antibody disrupts actin microfilaments and activates Rac1, which leads to increased expression of interleukin (IL)-1α and collagenase-1[68]. These studies provide additional support to the hypothesis that the ECM and nucleus regulate each other dynamically and reciprocally, that this balance occurs through the cytoskeleton, and that it is necessary for normal tissue architecture and function.

In addition to the biochemical signals originating from ECM receptors, the biophysical and mechanical properties of the tissue microenvironment are necessary for cell differentiation, function, and maintenance of architecture [69]. In vivo, tissues have intimate contact with their tissue-specific ECM, which in turn can greatly vary in ‘stiffness’ from one organ to another; for example, the ECM to which mammary epithelial cells adhere is much softer than the bone matrix where osteocytes reside [70]. Manipulating the ECM stiffness in culture dramatically changes gene expression profiles and rapidly reprograms cell differentiation. In sparse cultures of mesenchymal stem cells, matrices which mimic the stiffness of brain, muscle, and bone shift cell differentiation towards neuronal, myoblast, and osteoblast fates, respectively [69]. In mammary epithelial cells, endogenous milk protein expression is induced to an appreciable level when cells are cultured in floating collagen gels versus attached collagen gels [71], and we have recently demonstrated that the elasticity of floating collagen gels mimics the stiffness of the mammary gland in vivo, while attached collagen gels are greater than 3 fold stiffer [72]. Stiffness regulates acinar morphogenesis, as culturing mammary epithelial cells in ECM gels of comparable stiffness to the mammary gland leads to polarized acini with a central lumen, while increasing the gel rigidity enhances cell spreading and proliferation, inhibits cell polarization, and impedes tubulogenesis and lumen formation independent of altered matrix concentration [70, 73]. These studies elegantly demonstrate that tissue-specific architecture and function are regulated by the biophysical properties of the ECM, in particular matrix stiffness, independently and in addition to biochemical properties of the microenvironment.

Epithelial cell mechanics are largely determined by the biophysical and biochemical composition of the ECM substrata [74]. Mammary epithelial cells cultured on soft polyacrylamide gels acquire an elastic modulus similar to mammary tissue in vivo, and correspondingly express β-casein. However, increasing the matrix rigidity causes cell stiffening and spreading, which is accompanied by a dramatic reduction in β-casein expression, increased actin stress fibers, and increased phosphorylation of myosin II light chain [72]; this is in agreement with previous studies demonstrating that ECM-integrin signals are directly linked to the actinomyosin cytoskeleton [70]. Perturbing either actin polymerization or myosin II ATPase activity significantly blocks mechanotransduction from the substrata to cytoplasm and reduces cellular elasticity, indicating that actinomyosin contraction is a major contributor to this transmission [72]. An increase in cytoskeletal tension and substrata stiffness coordinates the activation of Rho/ROCK signaling, and treatment with a ROCK inhibitor reduces cellular elasticity and promotes acinar morphogenesis [70, 72]. Cell mechanics, in turn, also have a profound influence on the ECM microenvironment through regulating gene expression and ECM assembly: cytoskeletal tension can be transmitted through integrins to influence both fibronectin assembly [75, 76] and MMPs transcription [77], indicating that the cytoskeletal tension can actively regulate remodeling of the ECM microenvironment. This reciprocal mechanotransduction between the ECM and the cytoskeleton coordinates gene expression and tissue architecture, and serves as another plausible means by which the ECM regulates tissue structure and function dynamically and reciprocally. This balance is necessary for normal tissue homeostasis and tissue-specific function.

5 Conclusions

Individual tissues and organs within an organism vary dramatically in their cellular composition and overall architecture, and clearly they have different functional specificity. Even the most common types of cells from two discrete tissues display unique pattern of gene expression leading to distinct biological behavior despite identical genomic information. For instance, fibroblasts from two different locations (cutaneous versus visceral tissues) have distinct and characteristic transcriptional patterns [78]. Two recent studies from the laboratory of Gill Smith and his colleagues show that testicular cells and neuronal cells can differentiate into functional mammary epithelial cells after being transplanted into the mammary gland [79, 80]. These experiments strongly support the concept that the tissue microenvironment directs organ development and tissue specificity [81].

In this chapter, we have presented several lines of evidence illustrating the importance of the ECM in establishing the cellular architecture necessary for tissue-specificity. Biochemical and biophysical signals from the ECM polarize cells to induce tissue-specific differentiation by establishing an asymmetrical distribution of receptors and signal transduction components within cells, by activating transcription factors acting at ECM-response elements, and by remodeling the nuclear and chromatin structure globally and at specific loci. These signals depend on a cytoskeleton-regulated bidirectional signaling axis to bridge the ECM and nuclear compartments, as disruption of the cytoskeletal integrity prevents these ECM-induced events, and correspondingly, disrupts functional gene expression. In return, cells are able to influence the physical and biochemical properties of their microenvironment by regulating expression, assembly, and remodeling of their ECM. This dynamic and reciprocal interaction between the ECM and cells depends on the cytoskeleton to maintain the architecture necessary for tissue-specific function.

The deposition of ECM components and expression of ECM remodeling enzymes is subject to tight spatiotemporal regulation, reflecting the importance of finely-tuned ECM microenvironment in establishing the architecture necessary for proper function. ECM remodeling enzymes such as MMPs are able to modulate the tissue architecture within the context of normal organ development and biology, we and others have shown that forced expression of MMPs disrupts the normal tissue microenvironment leading to tumorigenesis in vivo [8285], suggesting that altering the fine balance of ECM and cell homeostasis by disrupting tissue architecture is sufficient in the long run not only to disrupt function, but also to induce tumorigenesis. Clearly the cytoskeleton is a critical player regulating cell and ECM homeostasis, but much more needs to be learned about the exact role and mechanism by which this complex and dynamic intracellular ‘matrix’ helps regulate tissue-specificity.


We apologize to those whose work could not be cited due to space limitations. This work was supported by the Office of Biological and Environmental Research of the Department of Energy (DOE-AC03-76SF00098), the National Institutes of Health (CA112970-01), (R01CA057621) to Zena Werb and M.J.B., (R01CA064786) to M.J.B. and the Breast Cancer Research Program (BCRP) of the Department of Defense (DOD) (Innovator Award) to M.J.B. M.JB. is a Distinguished Scientist of the OBER Office of the DOE. Support was also provided by a DOD BCRP postdoctoral fellowship DAMD17-02-1-0441 to R.X., a predoctoral fellowship W81XWH-05-1-0339 to A.T.B, and by a California BCRP Dissertation Award to ATB.


basement membrane
enhancer-binding protein
extracellular matrix
epidermal growth factor receptor
Janus kinase
laminin-rich ECM
matrix metalloproteinases
Phosphoinositide-3 kinase
poly(2-hydroxyethyl methacrylate)
signal transducers and activators of transcription protein 5
transforming growth factor-α
whey acidic protein


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