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Hepatol Int. Dec 2008; 2(4): 465–470.
Published online Sep 16, 2008. doi:  10.1007/s12072-008-9098-6
PMCID: PMC2716903
A 7-day profile of oxidative stress and antioxidant status in patients with acute liver failure
Vikram Bhatia, Payal Bhardwaj, Jessina Elikkottil, Jyoti Batra, and Anoop Sarayacorresponding author
Department of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences, New Delhi, 110029 India
Anoop Saraya, ansaraya/at/yahoo.com.
corresponding authorCorresponding author.
Received December 28, 2007; Accepted August 12, 2008.
Purpose
Acute liver failure (ALF) is characterized by a rapid and massive destruction of hepatocytes. The role of oxidative stress in perpetuating the injury is undefined and may be a potential therapeutic target. Our aim was to study serial variation in oxidative stress and antioxidant status in patients with ALF.
Methods
The study involved a prospective case–control study set in a tertiary care referral center. Thirty-two consecutive patients admitted with ALF were included with 23 healthy controls for comparison. Level of systemic oxidative stress as defined by superoxide dismutase (SOD), lipid peroxidation products (thiobarbituric acid reactive derivatives [TBARS]), and the total antioxidant capacity as the ferric reducing ability of plasma (FRAP) was measured at baseline on days 3 and 7.
Results
The patients were aged 24 years (range 13–60 years) and included 20 females. Thirteen (40.6%) patients died. Patients with ALF had significantly increased systemic oxidative stress at presentation, as reflected by higher levels of SOD (P < 0.001) and TBARS (P < 0.001) than controls. Both TBARS levels and FRAP decreased progressively from admission to the end of first week among the survivors (P = 0.004 and 0.015, respectively). The antioxidant status reflected by FRAP (P = 0.001) was significantly lower in ALF patients than controls. No relation was found between the level of oxidative stress and the mortality or complications.
Conclusion
A high level of systemic oxidative stress exists in ALF, with depletion of antioxidant reserves. Further studies are needed to define the clinical correlation of the large pro-oxidant burden.
Keywords: Acute liver failure, Oxidative stress, Antioxidant status profile
Acute liver failure (ALF) is characterized by a sudden and massive necrosis and apoptosis of hepatocytes. The main causes of ALF are viral hepatitis [13] or drug-induced liver toxicity [46]. Indirect evidence from animal studies suggests the role of reactive oxygen species and oxidative stress in the pathophysiology of ALF [710]. Oxidative stress has been implicated in a variety of liver diseases such as viral hepatitis [11, 12] and drug-induced hepatotoxicity [13, 14]. Furthermore, the dying hepatocytes can be a source of massive oxidative stress along with a depletion of antioxidant defenses. The oxidative stress can contribute to further loss of hepatocytes and impede regeneration, culminating in a vicious cycle. To the best of our knowledge, no human report is available, which has investigated this issue systematically. The aim of this study was to evaluate the role of oxidative stress when the liver fails acutely and to see the variation in oxidative stress and antioxidant levels as the liver injury progresses or resolves. We also sought to study the relation, if any, of the level of oxidative stress with the patient outcomes and complications.
Thirty-two consecutive patients with ALF admitted to the intensive care unit, Department of Gastroenterology and Human Nutrition, All India Institute of Medical Sciences (AIIMS), were prospectively included in this study. Also 23 age- and sex-matched healthy controls among hospital staff and patients’ relatives were enrolled for comparison of the oxidative stress and antioxidant levels. The controls were all nonsmoking and nonalcoholic healthy subjects free of any chronic illness. The median age of the controls was 20 years (range 20–58 years) and included ten females. According to the criteria laid down by the International Association for the Study of Liver, ALF was defined by the occurrence of encephalopathy within 4 weeks of onset of symptoms in the absence of any preexisting liver disease [15]. The diagnosis was confirmed by the presence of submassive or massive necrosis in the postmortem liver biopsy specimen of patients who died. Pre-encephalopathy period, icterus-encephalopathy period, grading of encephalopathy, clinical cerebral edema, and criteria for infection have been defined earlier [1618].
All patients were managed in the Department of Gastroenterology ICU, with standard organ support systems. A uniform management protocol was followed. Prophylactic antibiotics (piperacillin-tazobactam, vancomycin, and fluconazole) were instituted in each case. Cerebral edema was managed conservatively by bolus mannitol. Liver transplantation and liver replacement therapies were unavailable, and all patients were followed up until recovery or demise.
Viral markers
Serum sample from each patient was tested for hepatitis B surface antigen (HBsAg), IgM antibody against hepatitis B core antigen (IgM anti-HBc), and IgM antibody against hepatitis A virus (IgM anti-HAV) using commercial immunosorbent assay (ELISA) test kits (Organon, Teknika, Netherlands). IgM antibody to ORF-1, -2, and -3 of hepatitis E virus (HEV) and HEV RNA were tested by methods developed at our institute [19, 20]. Antihepatitis C (HCV) antibody was tested by using a third-generation commercial ELISA (Xcyton, Bangalore, India) test system.
An aliquot of 10 ml of blood was collected in plain and EDTA vials on days 1, 3, and 7 after admission among surviving patients. The serum and plasma samples were stored at −80°C for subsequent analysis. Ferric reducing ability of plasma (FRAP) was used as a marker of total antioxidant capacity (TAC)/antioxidant reserves, whereas thiobarbituric acid reactive substances (TBARS) and superoxide dismutase (SOD) were used as markers of oxidative stress.
Estimation of lipid peroxidation
TBARS was estimated by the method of Belch et al. [21]. Briefly 0.5 ml of serum was added to 40% trichloroacetic acid and centrifuged for 10 min at 2,500 rpm to precipitate proteins. From this, 0.5 ml of supernatant was taken and 0.5 ml of 0.67% thiobarbituric acid was added. Similar steps were taken for the standard (1,1,3,3-tetraethoxypropane). The tubes were covered and boiled for 1 h in boiling water bath. The tubes were than cooled immediately in chilled water bath and optical density (OD) was recorded at 530 nm. The results were calculated according to the standard graph.
Estimation of serum SOD
SOD assay was done following the auto-oxidation of pyrogallol [22]. Tris-EDTA (50 mM) buffer was prepared and its pH was adjusted at 8.5. Pyrogallol solution was prepared (20 mM) in 40 mM of hydrochloric acid. SOD enzyme was procured from Sigma, India. The change in OD ([increment]OD) for pyrogallol was set at 0.02–0.03 according to the sample. An aliquot of 50 μl of samples was diluted with 1,400 μl of buffer. Samples were then incubated at 37°C for 10 min, and 50 μl of adjusted pyrogallol ([increment]OD per minute = 0.03) working solution was added to the samples. The change in OD was recorded both at 1 min 30 s and at 3 min 30 s at 420 nm and was calibrated according to the standard graph.
Estimation of TAC
FRAP was estimated by the method of Benzie and Strain [23]. For the working FRAP reagent, we mixed 10 vol of acetate buffer, pH 3.6, 300 mmol/l; 1 vol of TPTZ (Sigma), 10 mmol/l in 40 mmol/l HCl; and FeCl3 · 6H2O (Sigma), 20 mmol/l. For the standard graph, FeSO4 · 7H2O (Sigma) was used. To 1 mL of the working FRAP reagent, 33 μl of sample was added and the change in OD was recorded at 30 s and 4 min. The results were calculated using the graph generated by the standard.
Data analysis and statistics
All continuous variables are expressed as median (range). Kruskal–Wallis test was used to compare measures of oxidative stress and antioxidant reserves between groups (controls, cases at days 1, 3, and 7). The Friedman test was used for nonparametric comparison of multiple-related samples within groups. The level of significance was set at P = 0.05. Statistical software SPSS (Version 10, SPSS Inc., Chicago, IL) was used for the statistical analysis.
The median age of the study patients was 24 years (range 13–60 years) and included 20 females. All patients were admitted within 3 days of onset of encephalopathy. Eleven of the females were pregnant. The baseline features of the study patients are detailed in Table 1. The complications of infection, renal failure, gastrointestinal tract bleeding, and seizures were seen in 20 (62.5%), 10 (31.3%), 3 (9.4%), and 7 (21.9%) patients, respectively. Thirteen (40.6%) patients died.
Table 1
Table 1
Baseline characteristics of the patients (n = 32)
Oxidative stress
Patients with ALF had significantly increased level of TBARS (nmol/ml) at baseline in comparison with that of healthy controls (P < 0.001). The TBARS level showed a significant decline from baseline to days 3 and 7 (P = 0.003) among surviving ALF patients (Figs. 1 and and2).2). The SOD (U/ml) level was significantly higher at baseline among ALF patients than healthy controls (P = 0.001). The level also gradually decreased toward day 7 among surviving patients, but was not statistically significant (Table 2).
Fig. 1
Fig. 1
TBARS significantly higher in cases on all days than controls (Kruskal–Wallis test, P < 0.001). TBARS significantly different on all days among cases (Friedman test, P = 0.003)
Fig. 2
Fig. 2
Serum SOD significantly higher in cases at baseline than controls (Kruskal–Wallis test, P = 0.001). Serum SOD not different among cases during 7 days (Friedman test, P = NS)
Table 2
Table 2
Oxidative stress and antioxidant levels over the initial week among the survivors (n = 11)
Antioxidant capacity
The FRAP level was significantly lower among ALF patients at baseline and on days 3 and 7 among survivors than healthy controls (P < 0.001). The FRAP levels showed a significant increase from baseline to day 7 (Fig. 3) among the survivors (P = 0.015).
Fig. 3
Fig. 3
FRAP significantly lower in cases on all days than controls (Kruskal–Wallis test, P < 0.001). FRAP significantly different on all days among cases (Friedman test, P < 0.015)
Among ALF patients, a significant negative correlation existed between the level of oxidative stress, as reflected by the TBARS levels, and the antioxidant capacity of plasma, as reflected by the FRAP levels at baseline (Spearman ρ = −0.452; P = 0.009). At days 3 and 7, this correlation between TBARS and FRAP levels was lost among the survivors (Spearman ρ = −0.266 and −0.358, respectively; P = 0.209 and 0.310, respectively). No correlation was, however, found between the SOD and the FRAP levels at baseline (Spearman ρ = 0.254; P = 0.160) and subsequently among the survivors. Among controls, the SOD and TBARS levels were unrelated to the FRAP levels (Spearman ρ = 0.208 and 0.248, respectively; P = 0.342 and 0.254, respectively).
Clinical correlation
No relation was found between the level of systemic oxidative stress or antioxidant levels, and either the patient outcome, or complications like cerebral edema, deeper encephalopathy grade, renal failure, or seizures (Table 3).
Table 3
Table 3
Systemic antioxidant levels and oxidant stress related to complications and outcome among ALF patients (n = 32)
Oxidative stress has been implicated in pathophysiology of various liver diseases. However, the role of oxidative stress has not been investigated in ALF in humans. The level of systemic oxidative stress was significantly higher among ALF patients as indicated by higher TBARS and SOD levels. The source of markers of oxidative stress is the ongoing hepatocyte injury and cell death. The lipid peroxidation products are liberated in the circulation as the membrane lipids are oxidized during cell lysis. These products are also a source of cytotoxicity and are suggested to invoke the inflammatory response in the body [24, 25]. Hence, an increased TBARS level may reflect the large-scale destruction of hepatocytes that may further aggravate the inflammation and cell death. The high SOD level may be a result of the overexpression of SOD mRNA, which is a normal physiologic response against the acute stress [2629]. Thus, the increased SOD levels may be a result of body’s adaptive response against the prevalent oxidative stress during ALF, which needs to be studied further by measuring expression of SOD gene in patients with ALF.
Acetaminophen toxicity, which is the most common etiology of ALF in the West, has oxidative stress as a key pathogenic factor for liver cell injury and antioxidant therapy as treatment. Acetaminophen toxicity leading to liver failure is mediated by hepatic glutathione depletion and mitochondrial oxidant stress [30]. No data are available on the intrahepatic or systemic oxidative stress among postnecrotic ALF. Among patients with nonalcoholic fatty liver disease (NAFLD) and alcoholic liver disease, both increased oxidative stress [31] and an immune response to lipid peroxidation products have been shown to aggravate liver injury [32].
Further support for the physiologic significance of the high oxidative stress is reflected by the depleted systemic antioxidant reserves. We found a significant negative correlation between the TBARS and the FRAP levels. Therefore, the levels of antioxidant reserves may be reduced by the increased utilization for quenching of the oxidant stress. Poor oral intake during the prodromal and hepatitis phase preceding the liver failure may also contribute to the depleted antioxidant reserves in these patients. Interestingly, among the survivors, the TBARS level decreased through the first week. This would reflect decreasing oxidative stress as the liver recovers. However, the oxidative stress at the end of first week still continued to be significantly higher than control levels. Also the antioxidant levels remained significantly lower even after 1 week among recovering patients, which indicates that there was a hyperdeficit of antioxidants during the acute phase of the disease.
We did not find a higher level of oxidative stress among the nonsurvivors. Also, we did not find any relation of oxidative stress with the complications of ALF. This is because oxidative stress may be a reflection of hepatocyte injury rather than having a causal role. The limitations of the present study include the small number of patients. A type-2 error might have underpowered the study to detect the clinical correlations of the level of oxidative stress. Another shortcoming was that normal levels for the assays among pregnant women were not known. Because a large number (11/20) of the females were pregnant, this might have influenced the comparisons. Finally, local milieu in the remaining hepatocytes might be different from the circulatory compartment. Perlemuter et al. [33] have recently reported that erythrocyte and plasma antioxidant defenses did not reflect intrahepatic peroxidation in patients with NAFLD. This discrepancy between the systemic and intrahepatic oxidative stress and the antioxidant defenses in the setting of ALF cannot be discounted. Future studies should compare the level of oxidative stress between ALF and severe hepatitis without liver failure.
In conclusion, this study suggested that there is a high level of systemic oxidative stress when the patient presents with ALF along with a deficit in antioxidant defenses. The correlation of oxidative stress with disease severity and clinical outcomes and the influence of antioxidants as a therapeutic modality in ALF need to be studied further.
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