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Conceived and designed the experiments: DGT ARB RDH TJW JJS MC MDA CDB AGC. Performed the experiments: DGT ARB RDH AI XH. Analyzed the data: DGT ARB RDH AI XH. Contributed reagents/materials/analysis tools: DGT ARB RDH AI XH CDB. Wrote the paper: DGT ARB RDH CDB AGC.
Analysis of polymorphism and divergence in the non-coding portion of the human genome yields crucial information about factors driving the evolution of gene regulation. Candidate cis-regulatory regions spanning more than 15,000 genes in 15 African Americans and 20 European Americans were re-sequenced and aligned to the chimpanzee genome in order to identify potentially functional polymorphism and to characterize and quantify departures from neutral evolution. Distortions of the site frequency spectra suggest a general pattern of selective constraint on conserved non-coding sites in the flanking regions of genes (CNCs). Moreover, there is an excess of fixed differences that cannot be explained by a Gamma model of deleterious fitness effects, suggesting the presence of positive selection on CNCs. Extensions of the McDonald-Kreitman test identified candidate cis-regulatory regions with high probabilities of positive and negative selection near many known human genes, the biological characteristics of which exhibit genome-wide trends that differ from patterns observed in protein-coding regions. Notably, there is a higher probability of positive selection in candidate cis-regulatory regions near genes expressed in the fetal brain, suggesting that a larger portion of adaptive regulatory changes has occurred in genes expressed during brain development. Overall we find that natural selection has played an important role in the evolution of candidate cis-regulatory regions throughout hominid evolution.
It has been suggested that changes in gene expression may have played a more important role in the evolution of modern humans than changes in protein-coding sequences. In order to identify signatures of natural selection on candidate cis-regulatory regions, we examined single nucleotide polymorphisms obtained from the complete re-sequencing of conserved non-coding sites (CNCs) in the flanking regions of over 15,000 genes in 35 humans. Patterns of allele frequencies in CNCs indicate the presence of both positive and negative selection acting on standing variation within these candidate cis-regulatory regions, particularly for the 5′ and 3′ UTRs of genes. Gene-specific tests comparing levels of polymorphism and divergence identify several genes with strong signatures of selection on candidate cis-regulatory regions and suggest that the biological characteristics of genes subject to selection are different between coding and candidate cis-regulatory regions with respect to gene expression and function. For example, we find stronger signatures of positive selection in candidate cis-regulatory regions near genes expressed in the fetal brain, which we do not observe in a concurrent analysis on protein-coding regions. Our results suggest that both positive and negative selection have acted on candidate cis-regulatory regions and that the evolution of non-coding DNA has played an important role throughout hominid evolution.
Over 30 years ago it was suggested that evolutionary changes at the level of gene regulation might have had a greater influence on human and chimpanzee phenotypic divergence than changes in proteins themselves . This is exemplified by humans and chimpanzees being highly similar at the protein level yet manifesting considerable phenotypic differences. Until recently, the focus of evolutionary studies has been on changes occurring within the protein-coding regions of genes, while evidence from Drosophila ,, rodents ,, and primates – have all suggested that Darwinian selection can be an important driving force of evolutionary change within non-coding DNA. Microarray studies have identified numerous transcriptional differences between human and chimpanzee –, and polymorphisms in cis-regulatory regions have been directly associated with differences in gene expression levels in humans ,. Therefore, single nucleotide polymorphisms (SNPs) in non-coding DNA can be associated with phenotypic differences, making them potential targets for natural selection.
A simple model of gene regulation involves the binding of transcription factors to several short non-coding sequences, which are generally found upstream of the transcribed regions of genes (forming a cis-regulatory region). Once a specific combination of transcription factors has bound to the DNA, the recruitment and assembly of the general transcriptional machinery will initiate gene transcription. However, gene regulation can also occur via additional processes, such as nucleosome positioning, distal enhancer elements, DNA methylation, and microRNA regulation. While a variety of computational methods have been developed to identify specific regulatory elements at the genomic scale, these methods can be prone to false positives due to the degeneracy and short size of DNA binding sites . An alternative predictor of regulatory function at the genomic scale may be the evolutionary conservation between species ,, although conservation scores can be prone to false negatives as not all functional sites are expected to be conserved ,. However, the study of conserved non-coding sites within regions potentially enriched for cis-acting elements does not a priori specify any particular mode of regulation, and may provide a broader glimpse of the evolutionary processes acting on candidate cis-regulatory regions.
We examined patterns of polymorphism and divergence in conserved non-coding sites (CNCs) in the flanking regions of 15,061 human genes, and the coding regions of 13,009 genes that were sequenced by Celera Genomics in 15 African Americans and 20 European Americans. We find evidence for selective constraint and adaptive evolution within candidate cis-regulatory regions, and find non-random patterns with respect to functional and transcriptional profiles of genes with higher probabilities of selection. Moreover, we find that patterns observed in candidate cis-regulatory regions are often distinct from those observed in protein-coding regions.
Our non-coding dataset included 6.9 Mb of autosomal conserved non-coding sites (CNCs) resequenced in 15 African Americans (AAs), 20 European Americans (EAs), and aligned to a single chimpanzee (PanTro2), while our coding dataset included 17.5 Mb of autosomal sites resequenced across the same panel of individuals, see ,. Conserved noncoding sites were defined as strictly non-coding sites that fall within human and mouse conserved sequences (sequences with at least 70% identity and 100 nucleotides in length). We found at least one human polymorphism or fixed difference to the chimpanzee in CNCs flanking a total of 11,334 autosomal genes (75.4%), with 7,826 genes having at least one human polymorphism across the 35 individuals (52.9%). CNCs that are flanking genes tend to have lower divergence and polymorphism compared to those in intergenic regions, however all categories of CNCs exhibit lower polymorphism and divergence as compared to synonymous sites (Figure 1). In AAs, CNCs in 5′ upstream regions have a smaller ratio of divergence to polymorphism when compared to synonymous sites, consistent with the presence of selective constraint in the upstream regions of genes (p=0.029, Table 1). However, the overall ratio of divergence to polymorphism is generally similar between pooled CNCs and synonymous sites (p>0.05, Table 1), and all categories of CNCs exhibit a higher ratio of divergence to polymorphism when compared to nonsynonymous sites.
The site frequency spectra of CNCs in both upstream and downstream regions of genes show a significant excess of lower frequency derived alleles (SNPs at a frequency of 1/16) as compared to both synonymous and intergenic sites (Figure 2 and Table 2), suggesting an excess of weakly deleterious alleles in CNCs in the flanking regions of genes. A study by Veyrieras et al.  has found that the majority of eQTLs lie either within or close to genes, suggesting that the excess of low frequency derived alleles observed in the flanking regions of genes may reflect the past action of negative selection on gene regulation. A shift in the distribution toward more rare alleles in CNCs is consistent with previous findings ,,,, and confirmed by significantly smaller values of Tajima's D in CNCs as compared to synonymous sites (D=−0.52 vs. −0.45 in AAs [Mann-Whitney U test, p<10−4], D=−0.31 vs. −0.21 in EAs [Mann-Whitney U test, p<10−6]) (Figure S1). Therefore, patterns in the site frequency spectrum suggest that CNCs in the flanking regions of genes have been subject to selective constraint.
Estimates of the population scaled selection coefficient (γ=2Nes) from the site frequency spectrum were calculated for different categories of CNCs using the program prfreq . All point estimates of γ were negative for CNCs in the flanking regions of genes as compared to intergenic CNCs, which share the same ascertainment scheme and show no evidence for selection when compared to synonymous sites (Table 3). We find a significantly better fit for a single estimate of γ when compared to a neutral model for all categories of CNCs, suggesting the presence of selective constraint that cannot be explained by the ascertainment of CNCs alone. Furthermore, all but the 5′ upstream and 5′ UTR regions of genes show a significantly better fit to a model with a distribution of γ, implying there is variation in the deleterious effects of mutations in CNCs. We also observe an excess of fixed differences that cannot be explained by a Gamma model of deleterious fitness effects, suggesting the presence of positive selection within CNCs in the flanking regions of genes. We estimate that 4.67% of the human-chimp fixed differences in CNCs can be attributed to positive selection, with UTRs showing more adaptive evolution (5′: 14.3%, 3′: 23.3%) than upstream or downstream regions (5′: 12.2%, 3′: 12.0%). Intronic CNCs show less evidence for positive selection (2.22%), but because they make up the majority of sites, pooled CNCs in the flanking regions of genes show modest evidence for adaptive divergence relative to intergenic CNCs.
We defined a candidate cis-regulatory region as all CNCs within the introns, UTRs, or within 5 kb up- or downstream of the transcription start or stop site of a gene. CNCs were pooled in this way to increase the power to detect selection, and to capture signals of selection without regards to a specific mode of cis-regulation. However, we note there is a significant correlation between the probability of selection estimated from the 5′ upstream regions and the combined set of CNCs (Figure S2). To identify genes whose candidate cis-regulatory regions may be under selection, contingency tables were constructed containing the counts of polymorphic sites and fixed differences to the chimpanzee in the pooled flanking CNCs of a gene. The total number of synonymous polymorphisms and fixed differences in coding regions (without respect to human and mouse sequence conservation) were pooled to use as a neutral standard as in ; the use of pooled vs. local synonymous sites has little effect on our estimates of selection (Figure S3). In order to identify loci showing signatures of natural selection, we implemented the program mkprf  by conservatively setting no fixed variance on the prior distribution of the population scaled selection coefficient (γ=2Nes), which shrinks the estimates of γ (Figure S4). For each gene we quantified the probability that the estimate of γ falls within five bins: γ<−1 (strong negative selection), −1<γ<−0.5 (weak negative selection), −0.5<γ<0.5 (nearly neutral), 0.5<γ<1 (weak positive selection), and γ>1 (strong positive selection) (Figure 3). In order to compare different modes of selection, the probability of negative selection was defined as Pr(γ<−0.5), and the probability of positive selection was defined as Pr(γ>0.5).
In order to control for population size changes that may affect our estimates of positive and negative selection, we incorporated demographic parameters when calculating the likelihood of our observed data in mkprf, the effect of which can be seen in Figure S5. A population expansion is expected to increase levels of polymorphism at neutral sites to varying extents due to differences in local mutation rates. In a set of neutral loci simulated under a model of population expansion, failing to correct for demography results in a neutral locus showing strong signatures of negative selection with credibility intervals on the mean estimate of γ below 0 (Table 4), and a slight inflation in the probability that γ<0 at higher values (Figure S6). Demographic models including a population expansion in African Americans and a single population bottleneck in European Americans were fitted to the complete set of autosomal synonymous SNPs using the program prfreq  and incorporated into mkprf. However, a single bottleneck model was found to be a poor fit to the European American sample, and even a complex, multi-bottleneck model could not account for the excess of high frequency derived alleles at synonymous sites . Therefore, we focus the majority of our conclusions on the African American sample. A complete list of genes including McDonald-Kreitman tables and mkprf results are available in Table S1, Table S2, Table S3, Table S4, Table S5, Table S6, Table S7, and Table S8.
In order to evaluate the performance of mkprf, we performed Wright-Fisher forward simulations under the inferred demographic model in African Americans using the program SFS_CODE . We find a significant correlation between simulated and estimated mean values of γ in datasets including various combinations of loci simulated under positive and negative selection (Figure S7). However, in a dataset including an equal number of loci simulated under positive and negative selection, the correlation is stronger for positively selected loci as compared to negatively selected loci, most likely reflecting a limited ability to distinguish between strong vs. weak negative selection. There is also a 10-fold increase in the number of genes returned with credibility intervals (CIs) on the mean estimate of γ>0 compared to CIs<0 (neut+del+pos, Table 4), as genes subject to strong enough negative selection often have reduced levels of polymorphism and divergence. Moreover, of the 11,000 simulated loci in the neut+del+pos dataset, 96% of loci in the positively selected class (γ>0) had at least 1 informative site, whereas only 94% of loci in the negatively selected class (γ<0) had at least 1 informative site making an estimate of γ even possible in mkprf. We note that across all datasets, only a limited number of the loci simulated under positive and negative selection have CIs on the mean estimate of γ above or below 0 (Table 4), suggesting there are likely additional loci subject to selection than identified by CIs alone.
An important consideration in gene specific tests for selection is unequal sequence coverage, as genes with a smaller number of resequenced sites tend to have fewer informative sites (fixed or polymorphic sites, Figure S8). It is unlikely that we have complete coverage of all cis-regulatory regions of a gene due to a focus on the 5′ upstream regions of genes, which is potentially problematic for our comparisons if genes with fewer informative sites were to provide less reliable estimates of γ. As discussed in the previous paragraph this may be particularly relevant for negatively selected loci, as genes subject to strong negative selection are expected to have fewer informative sites. Our simulations confirm that a higher percentage of negatively selected loci have fewer than 4 informative sites (neut+del+pos, 52%) as compared to either positively selected (16%) or neutral loci (28%). However, we find no significant difference in the distribution of γ when we compare loci with fewer or at least 4 informative sites when simulated under neutral, positive, and negative selection (Figure S9), suggesting that loci with fewer informative sites are unlikely to be problematic in our comparisons. The distributions of the number of resequenced sites at a locus in candidate cis-regulatory and protein-coding regions are shown in Figure S10.
We downloaded the Novartis Gene Expression Atlas 2 data from 72 normal human tissues in order to examine patterns of selection in candidate cis-regulatory regions with respect to gene expression signals . Microarray expression profiles were available for 87% of genes in our tests for natural selection. Conflicting studies have shown that expression patterns in tissue-specific genes evolve more rapidly , or more slowly  as compared to broadly expressed genes in comparisons of humans and mice. More recently, experimentally defined cis-regulatory regions were found to exhibit stronger degrees of selective constraint in genes expressed in a smaller number of tissues . However, we find little correlation between the probability of negative selection and the absolute number of tissues in which a gene is expressed (AA: Kendall's tau=−0.011, p=0.12; EAs: tau=−0.0060, p=0.40), nor with the index of tissue specificity , which includes additional information on the level of expression in each tissue (AA: Kendall's tau=0.0086, p=0.21; EAs: tau=0.0024, p=0.73). We also find no significant correlation between probabilities of selection and the mean and the maximum expression level of a gene across all tissues, suggesting that expression levels may not have a large impact on patterns of recent natural selection within candidate cis-regulatory regions. However, our simulations suggest that we likely have reduced power to identify significant correlations that are driven by negative rather than positive selection.
We then assigned genes to each tissue of expression in order to examine differences across tissues in evidence for selection on candidate cis-regulatory regions (Table S9, Table S10). Mann-Whitney U tests did not identify any tissues with a significantly higher probability of negative selection in either candidate cis-regulatory regions or nonsynonymous sites, suggesting that weak selective constraint may be a persistent factor affecting most human tissues, but likely reflects the limited power we have to detect negative vs. positive selection. On the other hand, we find that genes expressed in at least 3 tissues have a significantly higher probability of positive selection in candidate cis-regulatory regions (FDR<10%, Table 5), suggesting they may have an excess of positively selected loci. Notably, genes expressed in the fetal brain have a higher mean probability of positive selection as compared to genes expressed in other tissues, suggesting the importance of adaptive regulatory changes during brain development. Similarly, genes expressed in certain tissues of the adult brain, including the cerebellum peduncles and the medulla oblongata, also have a higher probability of positive selection in candidate cis-regulatory regions. Curiously, the medulla controls a variety of autonomic functions and is considered to be the most plesiomorphic structure of the brain , and might be expected to have a high level of conservation across species. However, the medulla also contains several motor nuclei important for facial expression, mastication, tongue movements, and controlling sound amplitudes that reach the inner ear, which are hypothesized to have played an adaptive role in the evolution of facial expression, feeding, and speech in humans .
Microarray studies have found that differences in brain expression patterns are more pronounced between humans and other primates as compared to other tissues , and that the majority of these differences are likely due to upregulation of brain-expressed genes in humans as compared to chimpanzees . However, these findings have been controversial , and a more recent study has found that differences in expression patterns between humans and chimpanzees are less pronounced in the brain as compared to heart, kidney, liver and testis . Regional expression in parts of the brain is generally conserved between human and the more distantly related mouse , raising the possibility that cognitive differences between species may be more likely to result from differential gene expression during development. While comparative microarray studies have generally focused on adult brain expression, our findings suggest that adaptive evolution might have had a larger impact on expression patterns in the fetal brain.
We applied the same methodology to examine differences in selection acting on coding regions with regards to gene expression. In contrast to what we observed for candidate cis-regulatory regions, we find no evidence for a higher probability of positive selection on nonsynonymous sites in genes expressed in the fetal brain in either AAs (Mann-Whitney U-test: p=0.26) or EAs (p=0.78). Our results are similar to a previous finding that positive selection in protein-coding regions is not elevated in genes expressed in the fetal brain . In light of our results, it would seem that adaptive evolution of fetal brain development is influenced more strongly by changes at the regulatory level rather than at the protein-coding level. However, we note that genes expressed in the “whole brain” and cerebellum show a trend towards higher probabilities of positive selection within nonsynonymous sites in AAs (p=0.03 and 0.048 respectively, FDRs=34%), suggesting that adaptive changes in the human brain may be the cumulative result of positive selection on regulatory regions during early development, and possibly on protein-coding regions in the adult brain.
Previous studies have identified immune response and T cell-mediated immunity as processes that are enriched for genes showing signatures of positive selection in protein-coding regions ,, highlight the importance of adaptive evolution in response to pathogens. We find that genes expressed in natural killer cells and T-cells both rank high among tissues with higher probabilities of positive selection in both coding and candidate cis-regulatory regions as compared to genes expressed in other tissues. Therefore, while genes expressed in the fetal brain show a different trend in coding and candidate cis-regulatory regions, positive selection in genes expressed in various immune cells may have occurred at both the coding and regulatory level.
Evolutionary patterns within candidate cis-regulatory regions can also provide insight into the relative importance of functional categories in the evolution of modern humans. We generated a custom GOslim set containing 129 terms from the Gene Ontology database , and performed Mann-Whitney U-tests to identify functional categories that have higher than expected probabilities of selection within candidate cis-regulatory regions (Table S11, Table S12). In AAs, functional categories with a higher probability of positive selection in candidate cis-regulatory regions include regulation of cellular process (GO:0050794, p=5.6×10−4, FDR=4%), protein modification (GO:0006464, p=4.8×10−3, FDR=9%), and cell cycle (GO:0007049, p=3.7×10−3, FDR=9%) (Table 6). In EAs, the most significant terms include calcium ion binding (GO:0005509, p=4.9×10−3, FDR=18%), organelle organization and biogenesis (GO:0006996, p=5.4×10−3, FDR=22%), cell cycle (GO:0007049, p=7.9×10−3, FDR=22%), and behavior (GO:0007610, p=9.3×10−3, FDR=22%). Functional categories with a higher probability of negative selection in candidate cis-regulatory regions in AAs are generally less significant than positive selection, but include cytosol (GO:0005829, p=8.6×10−3, FDR=16%), ribosome (GO:0005840, p=0.02, FDR=16%), extracellular region (GO:0005576, p=0.03, FDR=16%), and carrier activity (GO:0005386, p=5.9×10−3, FDR=22%), and in EAs include proteinaceous extracellular matrix (GO:0005578, p=0.01, FDR=19%), and extracellular space (GO:0005615, p=0.03, FDR=19%) (Table 6).
We tested the same group of GO terms in our parallel tests for selection on nonsynonymous sites within coding regions, and find that different categories are identified as having higher probabilities of selection in nonsynonymous sites as compared to candidate cis-regulatory regions (Table 6). Terms showing significantly higher probabilities of positive and negative selection in nonsynonymous sites correspond to previously published results involving the same dataset with AAs and EAs pooled . For example, we find that “transcription” has significantly higher probabilities of positive selection in nonsynonymous sites (GO:0006350; AA: p=1.1×10−3, FDR=8%; EA: p=2.5×10−3, FDR=6%), and that “actin binding” has higher probabilities of negative selection in nonsynonymous sites (GO:0003779: p=8.0×10−3, FDR=25%). Transcription factors have frequently been reported as having a high degree of positive selection in protein-coding regions in general ,,,, however this is not a trend that we observe in candidate cis-regulatory regions. Interestingly, in EAs we find that “calcium ion binding” has a significantly higher probability of negative selection at nonsynonymous sites (GO:0005509, p=1.5×10−3, FDR=6%), but within candidate cis-regulatory regions it shows a higher probability of positive selection (p=4.9×10−3, FDR=18%). Therefore, evolutionary patterns in candidate cis-regulatory regions tend to exhibit different functional patterns than those found in protein coding regions of genes.
We identified several genes that are likely to have undergone adaptive regulatory changes during human evolution (95% credibility interval on mean γ above 0). NUDT16 shows the strongest evidence for positive selection in candidate cis-regulatory regions in both AAs (Pr[γ>0.5]=98.5%) and EAs (Pr[γ>0.5]=97.4%). NUDT16 is a negative regulator of ribosome biogenesis, and may be involved in RNA decay in the nucleus. ITPR1 shows the second strongest evidence for positive selection in EAs (Pr[γ>0.5]=97.3%), however we find much weaker evidence for positive selection in AAs due to the presence of SNPs that are exclusive to AAs (Pr[γ>0.5]=70.7%). ITPR1 is essential for brain function, and is translated in response to synaptic activity in order to modulate calcium release from the endoplasmic reticulum. However ITPR1 also modulates calcium entry in the plasma membrane of B-cells, suggesting it may also have an important role in immunity. Functional studies have shown that the 3′ UTR of ITPR1 is required for dendritic localization in the mouse, however the majority of human-chimp fixed differences fall within conserved intronic sites rather than in the 3′ UTR region of this gene, suggesting that the target of selection is more likely to be an intronic cis-acting element (or elements). Within nonsynonymous sites, ITPR1 demonstrates little evidence for either negative or positive selection in both AAs and EAs, suggesting that positive selection on ITPR1 likely involved adaptive substitutions within candidate cis-regulatory regions rather than within protein-coding regions.
Additional genes with strong evidence for positive selection in candidate cis-regulatory regions in both populations include CACNA2D3, a calcium channel subunit protein ubiquitously expressed in fetal tissues; OR2L13, an olfactory receptor; and RNF167, a gene involved in protein degradation.
We also identified several genes that are likely to have experienced negative selection on candidate cis-regulatory regions (95% credibility interval on mean γ below 0). The gene with the highest probability of negative selection in candidate cis-regulatory regions in AAs is FREM1 (Pr[γ<−0.5]=98.4%), a gene involved in the development of a number of epidermal structures in the mouse , which also shows signatures of negative selection in EAs (Pr[γ<−0.5]=92.9%, but CI includes 0). FREM1 is highly expressed in the dermis during mouse embryonic development, and truncation of the FREM1 protein results in blebbing (blistering) diseases that are similar to phenotypes observed in Fraser syndrome, and dystrophic epidermolysis bullosa in humans. The gene with the highest probability of negative selection in candidate cis-regulatory regions in EAs in KRT40 (Pr[γ<−0.5]=99.9%), a hair keratin protein that shows a similarly high probability of negative selection in AAs (Pr[γ<−0.5]=97.6%). However, positive values of Tajima's D in both EAs (D=1.80) and AAs (D=1.32) indicate that polymorphisms at KRT40 tend toward intermediate frequencies, suggesting that KRT40 may potentially be subject to balancing rather than negative selection.
Other genes with strong evidence for negative selection in candidate cis-regulatory regions include SGCZ, a gene that may be important in the pathogenesis of muscular dystrophy and cardiomypathy; KIF19, a gene involved in intracellular transport and a member of a superfamily of proteins important for brain functioning; DOCK1, a gene thought to play a role in regulating phagocytosis during apoptosis; and TNNI3K, a cardiac-specific protein kinase. We also identify C14orf119 and C20orf117 as having strong evidence of negative selection in candidate cis-regulatory regions, however these genes have not been fully characterized.
In order to examine patterns of natural selection on candidate cis-regulatory regions with respect to human disease, we identified 666 Mendelian disease genes using a hand-curated list of genes from the Online Mendelian Inheritance in Man database (OMIM) , and 1,072 complex disease genes using the Genetic Association Database (GAD)  that were included in our scans for selection. We find that disease genes have a higher mean probability of negative selection within candidate cis-regulatory regions as compared to non-disease genes, however this trend is only suggestive in EAs, the population where the majority of diseases have likely been characterized (Mann-Whitney U-test; OMIM: p=0.23 in AAs, p=0.011 in EAs; GAD: p=0.29 in AAs, p=0.06 in EAs). A link between negative selection and human disease has also been observed in protein-coding regions of the genome , however genetic diseases can also be regulatory in nature .
There are several examples of disease-associated genes showing evidence for negative selection in candidate cis-regulatory regions in EAs. For example, LDB3, is a gene expressed in skeletal and cardiac muscle for which several nonsynonymous mutations have been associated with myofibrillar myopathy (OMIM:609452) and cardiomyopathy (OMIM:601493). Another gene, PLCE1 shows evidence for negative selection in both coding and candidate cis-regulatory regions, and homozygous mutations within the coding regions have been associated with type 3 nephrotic syndrome (OMIM:610725). Both LDB3 and PLCE1 show only moderate signatures of negative selection at candidate cis-regulatory regions in AAs.
On the other hand, there are several examples of disease genes that show strong evidence of positive selection in candidate cis-regulatory regions. For example, CACNA2D3 displays a pattern where loss of heterozygosity in intronic sequences is associated with renal cell carcinoma; CENTG3 variants confer protection against the pathogenesis of polyglutamine disease in the brain; and ALG3 variants are associated with congenital disorder of glycosylation type Id, a metabolic disease.
Direct comparisons between different classes of sites in mkprf may be confounded by differences in power to infer selection when there are varying degrees of selection on background loci in the dataset, particularly when there is no fixed variance on the prior distribution of γ. Our simulations show that as the proportion of negatively selected loci is varied, so does the number of genes showing strong signatures of positive selection due to a broader exploration of the parameter space (CI>0, Table 4), despite the actual number of positively selected loci remaining constant. For example, by increasing the number of negatively selected loci from 0 (neut+pos) to 699 (neut+wkdel+pos), to 1,485 (neut+del+pos), while keeping the number of positively selected loci constant (1,824 loci), the number of genes with CIs>0 changes from 206 to 199, to 203 (Table 4). If we increase the strength and the number of negatively selected loci to 4,632 loci (neut+stdel+pos), we then identify 267 loci with CIs>0 (Table 4). More importantly, the distributions of γ for positively selected and neutral loci show visible differences when the number of negatively selected loci is varied, which may confound direct comparisons when different classes of sites are analyzed independently (Figure S11). In order to control for varying levels of selection on background loci, we ran a combined analysis with nonsynonymous, synonymous, and candidate cis-regulatory regions in a single run of mkprf. The effect of analyzing different classes of sites in a concurrent vs. independent analysis is a shift in the distribution of γ towards smaller values for both candidate cis-regulatory regions and synonymous sites, and a shift in the distribution of γ towards larger values for nonsynonymous sites, causing the distributions of γ to be more similar, and more closely centered around 0 for different classes of sites (Figure S12).
First we looked for a correlation between selection acting on coding and candidate cis-regulatory regions, and observe what appears to be a complex relationship (Figure 4). In AAs we find a weak yet significant rank correlation between nonsynonymous and non-coding regions for both the probability of positive (Kendall's tau=0.055, p=1.6×10−11) and negative selection (tau=0.056, p=9.6×10−12), and between synonymous and non-coding regions (positive selection: tau=0.060, p=2.1×10−13; negative selection: tau=0.060, p=4.1×10−13). The correlation between synonymous and nonsynonymous sites appears to be slightly stronger (positive selection: tau=0.098, p<10−16; negative selection: tau=0.096, p<10−16), as expected due to increased linkage disequilibrium from a closer proximity between sites. Results from the EA sample are similar. However, given the small values of tau, identifying genes with evidence for selection in coding regions may be a poor predictor of whether a gene will show evidence for selection in the same direction as candidate cis-regulatory regions. A recent study has found that genes with patterns of expression consistent with any direction of selection (either positive or negative) exhibit reduced rates of protein evolution on nonsynonymous sites , which is consistent with only a weak correlation in the probability of selection between sites. The lack of a strong correlation in the mode of selection also suggests that the effect of linkage between candidate cis-regulatory and protein coding regions may be small, increasing the probability that we are detecting signatures of selection that are specific to non-coding regions.
We next compared the overall distributions of the probability of selection at different classes of sites (Figure 5). Nonsynonymous sites have a higher mean probability of negative selection as compared to both candidate cis-regulatory regions and synonymous sites (Mann-Whitney U-tests, p-values<10−16), and candidate cis-regulatory regions have a significantly higher mean probability of negative selection as compared to synonymous sites (p=8.7×10−4). We find that candidate cis-regulatory regions exhibit a significantly higher mean probability of positive selection as compared to nonsynonymous sites (p<10−16), however this is also true for synonymous sites (p<10−16), and synonymous sites have a marginally higher mean probability of positive selection as compared to candidate cis-regulatory regions (p=0.014). Although synonymous sites may not evolve under strict neutrality, they may better represent the distribution expected under neutrality while taking into consideration local effects of linkage, mutation rates, and variability in effective population sizes across the genome. Therefore, differences in the distributions of the probability of positive selection between nonsynonymous and candidate cis-regulatory regions may be driven by more neutral, rather than more adaptive evolution in candidate cis-regulatory regions.
However, it is possible we have limited power to identify differences in the degree of positive selection in protein-coding and candidate cis-regulatory regions, as the extent of positive selection within candidate cis-regulatory regions may be an underestimate due to their ascertainment. By restricting our analyses to human-mouse conserved sequences, it may have biased our dataset toward lower ratios of divergence/polymorphism based on neutral coalescent simulations (Text S1, Table S13, Table S14, Table S15, Figure S13, and Figure S14). For example, if we restrict our analysis of nonsynonymous sites to those within human-mouse conserved regions (i.e. use the same ascertainment as non-coding sites), all genes show strong evidence for strong selective constraint and have 95% credibility intervals below 0 on the mean estimate of γ (Table 4). Moreover, it is likely that only a small proportion of sites within candidate cis-regulatory regions are truly functional, as regulatory elements are often small. Therefore, the relative contribution of adaptive evolution at the level of gene regulation vs. changes in the actual protein remains an open question.
An important consideration is whether selection on synonymous sites has affected our estimates of selection. While we observe no discernable relationship between the probability of positive or negative selection with GC content (Figure S15), negative selection may indeed affect a certain proportion of synonymous mutations . Wright-Fisher simulations show that if purifying selection is acting on synonymous sites, we should expect a shift in the distribution of γ from a mean of 0.044 to 0.26 for a set of neutral loci (Table 4). On the other hand, selection on closely linked nonsynonymous sites may cause a reduction in polymorphism at synonymous sites, and could explain the signatures of positive selection we observe on synonymous sites (Table 4). Nevertheless, pooled synonymous sites provide a good fit to a neutral demographic model of expansion in the African American sample , and were used as a neutral standard in the same way for all classes of sites. Therefore, any bias is expected to have a similar affect on candidate cis-regulatory, synonymous, and nonsynonymous sites.
Our analysis of human polymorphism and divergence in conserved non-coding sites suggests that the evolution of candidate cis-regulatory regions is often driven by both positive and negative selection. Our findings reinforce the idea that the non-coding portion of our genome has an important functional and evolutionary role, and suggest that patterns of natural selection in non-coding DNA are often distinct from that of protein-coding regions. Many of the adaptive changes in candidate cis-regulatory regions might have occurred near genes expressed in the fetal brain, supporting the hypothesis that the evolution of the developing brain may be largely attributable to changes in gene regulation. Our results add to the increasing evidence that non-coding DNA is not all selectively neutral, and that selection on candidate cis-regulatory regions has played an important role throughout hominid evolution.
Sequencing and SNP detection was performed at Celera Genomics in 19 African Americans, 20 European Americans, and one chimpanzee as previously described . Primers were designed to amplify the protein-coding exons of 23,363 genes according to Celera's human genome version R26k, which concurrently amplified non-coding flanking sequences around each exon. Primers were also designed to target human and mouse conserved sequences (HMCS) within 5 kb upstream of the first start codon (for a total of 9,459 genes). HMCS were defined as sequences >100 bp and >70% identity between human and mouse, however the majority were between 250–500 bp in length.
The bioinformatic pipeline is depicted in Figure S16. We downloaded over 525,000 human and mouse conserved sequences (HMCS) from the UCSC genome browser , of which 87,100 were aligned to the trace sequences using BLAST . Sequences were oriented to hg17 using BLAT , of which 85,641 mapped to a unique position on the human genome. The positions of all but five HMCS were updated to hg18 using liftOver  with a ratio of remapped bases set to 1. A total of 83,379 HMCS were oriented to the public chimpanzee genome (PanTro2) using syntenic alignments from the UCSC genome browser. HMCS were annotated to genes according to Refseq 19 with respect to sites 5 kb upstream of the transcription start site, 5 kb downstream of the transcription stop site, 5′ UTR, 3′ UTR, intron, and coding. Sites beyond 5 kb upstream or downstream from any known transcript were annotated as intergenic. All sites within HMCS that overlapped with any known coding exons were masked, leaving strictly conserved non-coding sites (CNCs). Our resulting dataset included CNCs flanking a total of 15,061 autosomal genes. For comparisons to synonymous and nonsynonymous sites, we used the Celera Genomics exon resequence data from the same set of 35 people without regard to human and mouse conservation . In the case of alternatively transcribed genes, we counted the total number of fixed and polymorphic sites in nonsynonymous sites as the union of all known transcripts. An analysis of admixture revealed that 4 out of the 19 African American individuals had high levels of European ancestry , so these individuals were excluded from our study.
In order to estimate the distribution of fitness effects on conserved non-coding sites (CNCs) in the flanking regions of genes for African Americans, we calculated the likelihood that the observed site frequency spectrum fits a neutral model, a model with a single estimate of γ, and model with a Gamma distribution of γ using the program prfreq . Estimates of γ for intergenic CNCs were calculated relative to synonymous sites, and yielded no evidence for natural selection on intergenic CNCs. Estimates of γ for CNCs in the flanking regions of genes were then calculated relative to intergenic CNCs rather than synonymous sites in order to control for the effect of ascertainment based on conservation. Demographic parameters were simultaneously inferred from intergenic CNCs, which produced a similar model to that inferred from synonymous sites.
In order to identify candidate cis-regulatory regions subject to positive and negative selection we implemented the program mkprf ,, which estimates the posterior distribution of the population scaled selection coefficient (γ=2Nes) for individual loci. For a neutral comparison we pooled the number of fixed and segregating synonymous sites from the exon resequencing data, similar to the approach taken in Bustamante et al. . We updated the mkprf framework to incorporate the effect of non-stationary demography using the Poisson Random Field approach , and used the maximum likelihood demographic parameters from Boyko et al.  that were estimated from the frequency information at synonymous coding sites. In this model, the African population exhibited a 3-fold expansion approximately 6,800 generations ago whereas the European population exhibited a bottleneck followed by a more recent expansion. We implemented mkprf by conservatively setting no fixed variance on the prior distribution of γ, as the number of genes with credibility intervals (CIs) above or below 0 has been shown to be positively correlated to the variance set on the prior distribution of γ . For example, by not fixing the variance we identify 8 genes with CIs>0 and 7 genes with CIs<0 in our analysis of CNCs, whereas by fixing the variance to 8 we identify 11 genes with CIs>0 and 60 genes with CIs<0.
We performed Wright-Fisher forward simulations under the inferred demographic model for AAs using the program SFS_CODE  (Table 4). For each scenario a total of 11,000 loci were simulated using the length distribution of resequenced sites in candidate cis-regulatory regions (or synonymous sites), the observed mutation rate from pooled synonymous sites (θ=5.91×10−4), partial linkage between sites (rho=θ), and a splitting from the chimpanzee ancestor 20*2N generations ago to match the observed fixed/segregating ratio in pooled synonymous sites (N=7,778, the estimated ancestral population size estimated from synonymous sites ). Two sets of loci were simulated under a neutral demographic model (one for candidate cis-regulatory regions, one for synonymous sites), and three sets of loci were simulated under different selective regimes (one for candidate cis-regulatory regions, one for nonsynonymous, and one for synonymous sites). For candidate cis-regulatory regions a distribution of selective effects were drawn from a mixture of normal distributions assuming that most loci were under weak selection or nearly neutral (N=10,500, mean=0, s.d.=0.5), but with some loci having more extreme selection coefficients (N=500, mean=0, s.d.=5). This distribution assumed an equal number of genes exposed to positive and negative selection, and allowed us to evaluate our method under a general condition (neut+del+pos, Table 4). Loci with no informative sites were discarded, leaving a total of 9,863 loci in the neutral demographic set, and 9,707 loci in the neutral demographic+selection set that had at least 1 fixed or polymorphic site.
In order to evaluate our method under different conditions, we randomly substituted half of the negatively selected genes for neutral loci (neut+wkdel+pos), half of the negatively selected and all of the positively selected genes for neutral loci (neut+wkdel), all of the negatively selected loci for neutral loci (neut+pos), and all of the positively selected loci for neutral loci (neut+del). For nonsynonymous sites a distribution of selective effects was drawn from an exponential distribution with rate parameter 0.2, and truncated to the nearest integer. We then substituted all of the negatively selected loci that were simulated for nonsynonymous sites for an equivalent number of neutral loci in the neut+pos dataset (neut+stdel+pos). For synonymous sites a distribution of selective effects was drawn from an exponential distribution with rate parameter 0.8, reflected across the y-axis to be negative, and truncated to the nearest integer.
All correlations with gene attributes were run using the continuous distribution of the probability of positive and negative selection (Pr[γ>0.5] and Pr[γ<−0.5]). A Kendall's tau rank correlation coefficient was calculated in order to test for correlations between the probability of positive or negative selection and continuous biological variables. Mann-Whitney U-tests were used to test for differences in the mean probability of selection with discrete data, and multiple testing was considered using the false discovery rate  in the R statistical package using q.value. We created a custom GOslim including 129 terms, and annotated genes to the most terminal child to look for functional categories with higher probabilities of natural selection. In order to examine patterns of selection with regards to the transcriptional profiles of genes, we downloaded the Novartis Gene Expression Atlas 2 data  from 72 normal human tissues from the UCSC genome browser . A gene was considered to be expressed in a tissue if the signal was >350, and in the case of genes with multiple transcripts the average expression level for each tissue was calculated. The index of tissue specificity (τ) was estimated for each gene according to Yanai et al. .
Estimates of Tajima's D for different categories of sites; syn=synonymous sites, CNCs=conserved non-coding sites, repl=nonsynonymous sites. Notches represent the 95% confidence interval for the difference in two medians.
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Comparison of the probability of negative selection (above) and the probability of positive selection (below) when only CNCs in the 5′ upstream regions of genes are considered, as compared to when all CNCs are pooled for African Americans in mkprf.
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The effect of using pooled vs local synonymous sites on estimates of the probability of negative selection (above) and the probability of positive selection (below) for candidate cis-regulatory regions in the African American sample. The effect of pooling synonymous sites on estimates of γ is small, as in both cases mkprf uses only the information from fixed and polymorphic sites at candidate cis-regulatory regions in order to estimate the strength of selection.
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The effect of using a fixed variance of 8 vs. no fixed variance on the prior distribution of γ in candidate cis-regulatory regions. The probability of negative selection (top) is reduced above ~20%, while the probability of positive selection (middle) is reduced above ~50% when the variance is unfixed. Estimates of mean γ (bottom) demonstrate how not fixing the variance shrinks the estimate of the selection coefficient by restricting the size of the parameter space being explored.
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The effects of applying a demographic correction in mkprf on the inference of selection in candidate cis-regulatory regions in African Americans. The inclusion of a demographic model increases the variance in the posterior distribution of γ, which widens the distribution of estimated values of γ at a locus, inflating the probability that γ<−0.5 (top left) and the probability that γ>0.5 (bottom left). The probability that γ<0 is unaffected by a demographic correction in candidate cis-regulatory regions (bottom right). Estimates of the population scaled selection coefficient (γ=2Nes) are scaled by the current effective population size (Nc) under a model of population expansion, and by the time-averaged effective population size (N0) under a model of constant population size. In African Americans Nc>N0, resulting in a wider distribution of γ (top right) when a demographic correction is applied.
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The effects of applying a demographic correction in mkprf on the inference of selection on neutral loci simulated under a model of population expansion inferred from synonymous sites in African Americans. The inclusion of a demographic model increases the variance in the posterior distribution of γ, which widens the distribution of estimated values of γ at a locus, inflating the probability that γ<−0.5 (top left) and the probability that γ>0.5 (bottom left). The overall probability that γ<0 on neutral loci is slightly reduced at higher probabilities after correcting for demography.
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Correlations between simulated and estimated mean γ obtained from mkprf (see Table 4 in the main text). Kendall's tau rank correlation tests all have p-values<10−16 (except for neut). Loci simulated under positive selection show a stronger correlation than loci simulated under negative selection in the neut+del+pos dataset: tau=0.38 for positively selected loci (p<10−16), and tau=0.071 for negatively selected loci (p=6.6×10−3). However, in the neut+stdel+pos tau=0.27 for positively selected loci (p<10−16), and tau=0.33 for negatively selected loci (p<10−16).
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The relationship between the total number of sites resequenced in candidate cis-regulatory regions (total sites), and the total number of fixed and polymorphic sites (informative sites). We find a significant correlation between estimates of γ and total sites when all genes are considered (Kendall's tau=0.043, p=3.3×10−11). The correlation disappears if we condition on genes having at least 4 informative sites (tau=−0.0048, p=0.60).
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Distribution of mean γ estimated with mkprf for loci simulated under negative (top), positive (middle), and neutral evolution (bottom) in the neut+del+pos dataset (see Table 4 in the main text). “Informative” genes are those with at least 4 fixed or polymorphic sites, and “noninformative” genes are those with <4 fixed or polymorphic sites. Most of the distributions are not significantly different (Mann-Whitney U-tests, p>0.05), with the exception of neutral loci where informative genes have a slightly higher mean γ (0.69 vs 0.61, p=0.02). We obtain similar results for the neut+stdel+pos dataset. We note that genes simulated under smaller values of γ are less likely to be in the informative class.
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Distribution of the number of sites resequenced in candidate cis-regulatory and nonsynonymous sites.
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Distribution of estimated mean γ for simulated neutral and positively selected loci analyzed in mkprf with an increasing number of loci simulated under negative selection (see Table 4 in the main text) when there is no fixed variance on the prior distribution of γ. The distributions are significantly different between the neut+pos and the neut+stdel+pos for positively selected loci (Mann-Whitney U-test, p -values<10−16), and are significantly different between all datasets for neutral loci (p -values<10−16).
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The effect of analyzing different classes of sites in a separate, independent run of mkprf vs. a concurrent run including all classes of sites. Simulations suggest that the difference across runs is due to the effects of varying degrees of selection in the background loci, which if not controlled for may be a confounding factor when comparing the extent of natural selection between different runs of mkprf.
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Boxplots showing the log of the ratio of the number of polymorphisms to the number of human-chimpanzee fixed differences for unfiltered and HMCS simulated data.
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The distribution of LI for the simulated HMCS and unfiltered datasets.
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The relationship between GC content and the probability of positive and negative selection in candidate cis-regulatory regions, nonsynonymous, and synonymous sites in AAs. GC content in candidate cis-regulatory regions shows a weak, but significant negative rank correlation with the probability of negative selection (Kendall's tau=−0.014, p=0.029), and a weak, but significant positive rank correlation with the probability of positive selection (tau=0.013, p=0.043). The correlation between GC content and selection on nonsynonymous sites also appears to be weak (negative selection: tau=−0.024, p=4.8×10−4; positive selection: tau=0.025, p=1.9×10−4), similarly for synonymous sites (negative selection: tau=−0.021, p=0.0033; positive selection: tau=0.016, p=0.024). Results are similar in EAs.
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Flowchart of the bioinformatic pipeline.
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McDonald-Kreitman tables for candidate cis-regulatory regions in AAs.
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McDonald-Kreitman tables for candidate cis-regulatory regions in EAs.
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McDonald-Kreitman tables for protein-coding regions in AAs.
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McDonald-Kreitman tables for protein-coding regions in EAs.
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mkprf results for candidate cis-regulatory regions in AAs.
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mkprf results for candidate cis-regulatory regions in EAs.
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mkprf results for nonsynonymous sites in AAs.
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mkprf results for nonsynonymous sites in EAs.
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GeneAtlas2 results for candidate cis-regulatory regions.
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GeneAtlas2 results for protein coding regions.
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Gene Ontology data for candidate cis-regulatory regions.
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Gene Ontology data for nonsynonymous sites.
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Summary statistics for the log of the ratio of polymorphism/divergence in simulated human-mouse conserved sequences versus unfiltered sequences.
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Summary statistics for the distribution of the log of the neutrality index for simulated human-mouse conserved sequences versus unfiltered sequences.
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Proportion of simulations with no polymorphisms or human-chimpanzee fixed differences in simulated human-mouse conserved sequences and unfiltered sequences.
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We would like to thank the Celera Genomics sequencing centre for generating the data, and David Lawrie, Rasmus Nielsen, Sarah Stockwell, and members of the Clark and Bustamante labs for many helpful discussions and technical assistance. We are also extremely grateful to three anonymous reviewers for their invaluable comments.
The authors have declared that no competing interests exist.
This work was supported in part by National Institutes of Health grants HL084706, HG003229, and MH084685 to AGC and CDB; MICORTEX grant P50 GM065509 to AGC; and a Natural Sciences and Engineering Research Council of Canada Postdoctoral Fellowship to DGT. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.