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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Immunol. Author manuscript; available in PMC 2010 April 1.
Published in final edited form as:
PMCID: PMC2709763
NIHMSID: NIHMS96146

Uneven colonization of the lymphoid periphery by T cells that undergo early TCRα rearrangements1

Abstract

A sparse population of thymocytes undergoes TCRα gene rearrangement early in development, before the double positive stage. The potential of these cells to contribute to the peripheral T cell pool is unknown. To examine the peripheral T cell compartment expressing a repertoire biased to early TCR gene rearrangements, we developed a mouse model in which TCRα rearrangements are restricted to the double negative stage of thymocyte development. These mice carry floxed RAG2 alleles and a Cre transgene driven by the CD4 promoter. As expected, conventional T cell development is compromised in such Cre(+) RAG2fl/fl mice, and the TCRαβ+ T cells that develop are limited in their TCRα repertoire, preferentially utilizing early-rearranging Vα genes. In the gut, the Thy-1+TCRαβ+ intraepithelial lymphocyte (IEL) compartment is surprisingly intact, while the Thy-1TCRαβ+ subset is almost completely absent. Thus, T cells expressing a TCRα repertoire that is the product of early gene rearrangements can preferentially populate distinct IEL compartments. Despite this capacity, Cre(+) RAG2fl/fl T cell progenitors cannot compete with wild-type (WT) T cell progenitors in mixed bone marrow chimeras, suggesting that in normal mice, there is only a small contribution to the peripheral T cell pool by cells that have undergone early TCRα rearrangements. In the absence of WT competitors, aggressive homeostatic proliferation in the IEL compartment can promote a relatively normal Thy-1+ TCRαβ+ T cell pool from the limited population derived from Cre(+) RAG2fl/fl progenitors.

Keywords: T cells, T Cell Receptors, Repertoire Development, Gene Rearrangement, Mucosa

Introduction

The development of mature αβ T lymphocytes in the thymus depends on successful rearrangement of variable (V), diversity (D) and joining (J) gene segments to encode a functional αβ TCR. V(D)J gene recombination requires RAG1 and RAG2 gene products and is a tightly regulated, orderly process. Tcrb rearrangements occur first at the CD8CD4 double negative (DN)3 stage of thymocyte development. Successful rearrangement and expression of a TCRβ chain that can pair with the pre-Tα chain drives a burst of proliferation and progression to the CD8+CD4+ double positive (DP) stage, through a process termed β-selection. Within the DP compartment, Tcra genes are rearranged, often on both alleles. This flexibility allows for multiple attempts at creating a functional αβ TCR, one that can promote positive selection, lineage commitment, loss of RAG expression and cessation of further gene rearrangements.

The complex organization of the murine TCRα/δ , with Dδ and Jδ genes situated between V and Jα gene segments, ensures that Tcrd genes are excised upon Vα-Jα gene rearrangement. Regulation of TCRα gene rearrangement is orchestrated by various promoters and enhancers that determine when rearrangement occurs and which V and J gene segments are used (1, 2). Primary, or early, Vα gene usage is biased to the 3’ end of the V gene array. Vα6 and Vα20 are at the 3’ end of the locus and are preferentially utilized in early rearrangements, while Vα12 and Vα19 are at the 5’ end and tend to be used in late rearrangements. In addition, Vα-Jα gene rearrangement is biased, with 3’ Vα genes preferentially rearranging to proximal 5’ Jα genes (3, 4).

While the majority of Tcra gene rearrangements occur in the DP stage, Tcra genes can be rearranged in early thymocytes, and transcripts have been found in the DN2, DN3 and DN4 compartments (5, 6). Although pTα−/− mice have thymic hypoplasia, they do have peripheral TCRαβ+ T cells, showing that TCRα chains expressed at the DN stage can pair with TCRβ chains to drive β-selection in the absence of pre-Tα expression (7). The timing of TCRα rearrangement may be a factor in lineage commitment. Some evidence suggests that premature expression of TCRαβ may divert cells into the TCRγδ lineage (8). In addition, aberrantly early TCRα expression in HY TCR transgenic mice promotes the expansion of the TCRαβ CD8αα+ subset of IELs in the gut (9). Delaying TCRα expression until the DP stage prevents the dramatic expansion of this compartment (10), suggesting that IELs may be the progeny of thymocytes that have undergone early TCRα rearrangements. In support of this notion, the IEL compartment is seeded early in development, a time when early-rearranging TCRs are overrepresented (4). TCRγδ+ and TCRαβ+ IELs are found in neonatal mice and in human fetal intestine (11). The rapid development of IELs is also supported by the fact that, in bone marrow radiation chimeras, repopulation of the IEL compartment occurs earlier than in the spleen or thymus (12). Based on these observations, we hypothesized that T cells bearing the products of early TCR rearrangements may constitute a specific T cell subset with the ability to populate certain compartments (such as IELs) preferentially.

To test this hypothesis and analyze the impact of a repertoire biased to early TCR gene rearrangements, we developed a mouse model in which TCR rearrangements are restricted to DN thymocytes. We crossed mice carrying floxed RAG2 alleles (13) with mice that are transgenic for Cre driven by the mouse CD4 promoter/enhancer/silencer (14). In Cre(+) RAG2fl/fl mice, Cre expression is initiated in the late DN3 stage (15), leading to deletion of RAG2 by the early DP stage. Therefore, T cells are able to progress efficiently through Tcrb gene rearrangement and β-selection but have a narrow window of opportunity in which to make productive TCRα rearrangements.

Analysis of Cre(+)RAG2fl/fl mice reveals that the TCRαβ+ T cell population in the spleen and peripheral lymph nodes (LNs) is significantly diminished, with an inversion of the CD4:CD8 ratio. In addition, the TCRα repertoire is oligoclonal, with preferential representation of TCRs utilizing early-rearranging Vα genes. In the small intestinal IEL compartment, however, the Thy-1+TCRαβ+ population is intact, while the Thy-1TCRαβ+ population is nearly absent. In mixed chimeras, cells derived from WT bone marrow outcompete those derived from Cre(+) RAG2 fl/fl bone marrow, even in the gut. Thus, in the absence of WT competitors, aggressive homeostatic proliferation allows preferential repopulation of certain T cell compartments in the small intestine by cells expressing TCRs that are the products of early TCRα rearrangements.

Materials and Methods

Mice

RAG2fl/fl mice on the C57BL/6J (B6) background were a gift from K. Rajewsky (The CBR Institute for Biomedical Research) and were crossed to CD4p-Cre transgenic (C57BL/6NTac-TgCd4-cre) B6 mice (hereafter “Cre(+) RAG2+/+”), a gift from C. Wilson (University of Washington). Mice were bred to homozygosity. WT Ly5.2+ B6 and Ly 5.1+ B6.SJL (B6.SJL-PtprcaPepcb/BoyJ) mice were purchased from The Jackson Laboratory or bred in-house, and TCRα-deficient (Tcratm1Mom) mice were a gift from A. Rudensky (University of Washington). Controls were either Cre(−) RAG2fl/fl, Cre(+) RAG2+/+, or WT B6 mice, as a comparative analysis revealed no differences in cell numbers or phenotype between these strains (data not shown). All mice were bred and housed under specific pathogen-free conditions in accordance with institutional guidelines. Mice were used at 6–10 weeks of age, except bone marrow chimeras, which were used at 8–20 weeks of age. All experiments were performed in compliance with the University of Washington Institutional Animal Care and Use Committee.

Mixed bone marrow chimeras

Bone marrow was obtained from Cre(+) RAG2fl/fl (Ly5.2) and B6.SJL (Ly5.1) donors and was depleted of mature T cells using the EasySep biotinylated separation kit (Stem Cell Technologies) and the following biotin-conjugated antibodies: anti-CD4 (Rm4.4), anti-CD8 (53–6.7) and anti-Thy1.2 (30-H12), all from BD Biosciences. B6×B6.SJL (Ly5.1×5.2) hosts were lethally irradiated with 1000 rads at least 7 hours prior to reconstitution. Bone marrow was mixed 1:1 and 4×106 cells were injected in the lateral tail vein in 200µL sterile PBS. Hosts were maintained on antibiotic water (polymyxin B sulfate and neomycin sulfate) for 2 days prior to and 14 days post irradiation. Chimeras were analyzed >8 weeks post-injection.

Flow cytometry antibodies

Antibodies used to stain cell surface molecules were: PE-conjugated anti-CD19 (1D3), anti-CD25 (PC61) and anti-CD103 (M290), Allophycocyanin-(APC) conjugated anti-Thy1.2 (CD90.2; 53-2.1) and anti-CD62L (MEL-14), PerCP-, PE-Cy7- and APC AlexaFluor 750-(APCAF750) conjugated anti-CD4 (RM4–5), FITC- and PerCP-conjugated anti-CD8α (53-6.7), FITC-conjugated anti-pan TCRβ (H57-597.13) and anti-TCRγδ (GL3), biotin-conjugated anti-CD8.2β (53-5.8), PE- and PE-Cy7-conjugated anti-CD69 (H1.2F3), PE-Cy7- and APC-conjugated anti-CD44 (Pgp-1), PE-Cy7-conjugated anti-Ly5.1 (A20), and FITC- and APCAF750-conjugated anti-Ly5.2 (104), all from BD Biosciences or eBioscience. APCAF750-conjugated anti-CD8α (5H10) and PE-conjugated Streptavidin were from Caltag. TL-tetramer, used to detect CD8αα, was a gift from H. Cheroutre (La Jolla Institute for Allergy and Immunology) and was conjugated to PE-labeled Streptavidin (Molecular Probes).

Cell preparation

IELs were prepared as previously described with a few modifications (16). Briefly, small intestines were removed and all mesenteric fat cleaned. Peyer’s patches (PPs) were removed and processed. Intestines were opened longitudinally and washed of their contents. Intestines were cut into 0.5cm pieces and stirred at 450 rpm at 37°C for 20 min in CMF medium [HBSS (Ca++ and Mg++ free), 100mM HEPES, 250mM sodium bicarbonate, 2% FCS and 0.154mg/mL dithioerythriol]. Cell suspensions were filtered, washed and resuspended in 44% Percoll, underlaid with 67% Percoll, and centrifuged at 2800rpm for 20 min. Enriched IELs were extracted from the interface and washed. IEL preps were free of contaminating PP lymphocytes, as they consistently contained <1.3% CD19+ B cells. IELs, thymus, LNs (axial, brachial, inguinal and mesenteric), PPs and water-lysed spleen cells were processed using 70µM nitex filters and resuspended in HBSS/1%BSA. Single-cell suspensions were incubated with anti-CD16/CD32 blocking Ab (2.4G2; BD Biosciences) and were stained according to standard procedures. Cells were analyzed on a FACSCanto flow cytometer (Becton-Dickinson, San Jose, CA) and data were analyzed using FlowJo software (TreeStar Inc, Ashland, OR). For some experiments, spleen and LN T cells were enriched by negative selection using the EasySep Mouse T cell Enrichment kit (StemCell Technologies) and stained with antibodies to CD4, CD8α and TCRβ. Cells were sorted on a FACSAria cell sorter (BD Biosciences) to >97% purity. Where indicated, purified T cells were incubated with 5mM CFSE (Molecular Probes) for 10 min at 37°C and then washed twice in ice cold complete RPMI 1640 medium with 10% FCS before being placed in culture.

In vitro stimulation and intracellular cytokine staining

CFSE-labeled, purified CD4+ and CD8+ T cells from spleens and LNs of WT and Cre(+)RAG2fl/fl mice were cultured with anti-CD3/anti-CD28 Dynabeads (Invitrogen) at a 1:1 bead:cell ratio. Cells were harvested on days 2 or 3 and restimulated for 5 h with 50ng/ml PMA (Sigma) and 0.7µM ionomycin (Sigma) in the presence of the protein transport inhibitor Brefeldin A (BD Golgiplug, BD Biosciences). Washed cells were surface stained with anti-CD4 or anti-CD8α for 20 min at 4°C and then washed. Intracellular cytokine staining was performed using the BD Cytofix/Cytoperm buffers according to the manufacturer’s protocol.

Quantitative RT-PCR

Total mRNA was extracted from purified cells using the RNeasy mini kit (Qiagen) and first-strand cDNA synthesis was performed with oligo(dT) primers, using SuperScript III (Invitrogen) according to the manufacturer’s directions. RNase-treated cDNA was subjected to quantitative PCR performed on an ABI 7300 Real Time PCR System (Applied Biosystems, Foster City, CA) using Power SYBR Green PCR Master Mix (Applied Biosystems). PCRs consisted of a 10 min 95°C denaturation step followed by 45 cycles of 15 s at 95°C and 30 s at 60°C. The following primers were used: Vα6 [TRAV21, IMGT nomenclature] sense primer, 5’-ATGGCTTTCCTGGCTATTGC-3’, Vα12 [TRAV2, IMGT nomenclature] sense primer, 5’-CTGTTTATCTCTGCTGACCGG-3’, Vα19 [TRAV1, IMGT nomenclature] sense primer, 5’-TGGATGGTTTGAAGGACAGTG-3’, Vα20 [TRAV19, IMGT nomenclature] sense primer, 5’-GGAAGACGGAAGATTCACAGTT-3’, Cα anti-sense primer, 5’-TGGCGTTGGTCTCTTTGAAG-3’, hypoxanthine guanine phosphoribosyltransferase (HPRT) sense primer 5’-GTTGGATACAGGCCAGACTTTGTTG-3’, and HPRT anti-sense primer 5’-GAGGGTAGGCTGGCCTATAGGCT-3’. Reactions were run in triplicate and samples were normalized to the internal HPRT control.

TCR CDR3 length spectratyping

CDR3 length spectratyping was performed as previously described by with a few modifications (17). Primary PCR reactions were performed using the following previously described primers (18, 19): Vα6 sense primer, 5’-ATGGCTTTCCTGGCTATTGCC-3’, Vα12 sense primer, 5’-TCTGTTTATCTCTGCTGACC-3’, Vα19 sense primer 5’-TGCCTCATACCTCTGTGCTG-3’, Vα20 sense primer, 5’-TTCTCACTGCACATCACAGC-3’, and the Ca anti-sense primer as above. Cycling conditions were: 94°C for 45 s, 55–60°C for 45 s and 72°C for 1 min for 35 cycles. Secondary run-off PCR reactions using 25% of the primary product were performed using a Fam-labeled Ca anti-sense primer: 5’-ACACAGCAGGTTCTGGGTTC-3’. Cycling conditions were: 94°C for 30 s, 58°C for 30 s and 72°C for 1 min for 10 cycles. Run-off products were separated on the basis of length by the Genomics Facility at the Fred Hutchinson Cancer Research Center (Seattle, WA). Using the Genescan 3.7 software (Applied Biosystems), each PCR product is resolved as peaks, with each peak representing TCRs of different CDR3 length (in base pairs). Data were quantitated by determining the total peak height for each Vα-Cα pairing and expressing each fragment as a percentage of total height.

Results

Post-selection thymocyte development is severely impaired in Cre(+) RAG2fl/fl mice

Flow cytometric analysis of thymocytes from Cre(+) RAG2fl/fl, Cre(−) RAG2fl/fl control, and TCRα-deficient (TCRα−/−) mice reveals that, while DN and DP compartments and total thymic cellularity are similar, there is a drastic reduction in both the percent and number of CD4 and CD8 single positive (SP) thymocytes in Cre(+) RAG2fl/fl mice, similar to that seen in mice lacking TCRα (Figure 1A and B and data not shown). In WT mice, the CD8loCD4lo population (“mature” DP) contains a high percentage of TCRαβ+CD69+ thymocytes that have undergone positive selection (20). In Cre(+) RAG2fl/fl mice, there is a greatly reduced percentage of mature DP thymocytes in comparison to controls, suggesting that the majority of DP thymocytes in these mice do not complete positive selection (Fig. 1C and D).

Figure 1
Thymocyte development is impaired in Cre(+) RAG2fl/fl mice

Some CD4+CD8αβ+ DP thymocytes also express the CD8αα+ homodimer and give rise to the TCRαβ+CD8αα+IEL population (21). Using tetramers of the thymic leukemia (TL) antigen, that bind specifically to CD8ααand not CD8αβ(22), we observed that the percentage of CD4+CD8αβ+CD8αα+triple positive (TP) thymocytes in Cre(+) RAG2fl/fl mice is not significantly different from that in Cre(−) RAG2fl/fl control mice (Fig. 1E and F). Thus, while the thymic precursor pools of TCRαβ+ peripheral and gut T cells remain intact, the post-positive selection thymocyte compartment is greatly diminished in Cre(+) RAG2fl/fl mice.

The TCRαβ+ T cell compartment in secondary lymphoid organs is significantly reduced in Cre(+) RAG2fl/fl mice

Although thymic output is greatly reduced in Cre(+) RAG2fl/fl mice, there is a small peripheral TCRαβ+ T cell compartment, comprising ~5–7% of total splenocytes (Fig. 2). A similarly reduced compartment is found in peripheral LNs and mesenteric LNs (MLNs) (data not shown). The cellularity of secondary lymphoid organs is reduced in Cre(+) RAG2fl/fl mice, a result of the small T cell compartment, as CD19+ B cell numbers in both spleen and LNs (including MLNs) are similar in Cre(+) RAG2fl/fl and Cre(−) RAG2fl/fl control mice (Fig. 2A and B and data not shown). Within the Thy-1+ compartment, the number of TCRγδ+ T cells is similar in Cre(+) RAG2fl/fl and Cre(−) RAG2fl/fl control mice (Fig. 2C). Interestingly, within the Thy-1+TCRαβ+ T cell compartment, the percentage of CD8+ T cells is similar to that of Cre(−) RAG2fl/fl control mice. In contrast, there is a ~50% reduction in the CD4+ subset, leading to a reduced CD4:CD8 ratio (Fig. 2D and data not shown). Flow cytometric analysis reveals that Thy-1+TCRαβ+ CD4+ and CD8+ splenocytes from Cre(+) RAG2fl/fl mice are CD44hi (Fig. 2E). The CD4+ T cell subset also contains a population that is CD62Llo, while the CD8+ T cell subset does not. Thus, the TCRαβ+ (but not the B cell or TCRγδ+) peripheral compartment is diminished in Cre(+) RAG2fl/fl mice and the remaining cells bear an activated/memory phenotype.

Figure 2
The peripheral TCRαβ+ T cell compartment is compromised in Cre(+) RAG2fl/fl mice

T cells from Cre(+) RAG2fl/fl mice are hyperproliferative and produce high levels of IFN-γ upon stimulation

We next assessed whether splenic and LN T cells from Cre(+) RAG2fl/fl mice are functional. Within 48 hours of anti-CD3/anti-CD28 stimulation, both CD4+ and CD8+ T cells from Cre(+) RAG2fl/fl mice have proliferated more than cells from WT mice (Fig. 3A). By day 3, most Cre(+) RAG2fl/fl T cells have undergone more than 4 divisions. In addition to being hyperproliferative, the proportion of IFN-γ producers among Cre(+) RAG2fl/fl T cells is 2 to 5-fold higher than that of the WT cultures (Fig. 3B).

Figure 3
Cre(+) RAG2fl/fl T cells are hyperproliferative and display rapid effector function upon stimulation

TCRαβ+ T cells in Cre(+) RAG2fl/fl mice display a biased TCRα repertoire

Quantitative RT-PCR on RNA from purified CD8+ and CD4+ T cells reveals that, while Vα6 and Vα20 expression levels are higher in Cre(+) RAG2fl/fl T cells compared to WT, Vα12 and Vα19 expression levels are significantly lower (Fig. 4A). All four genes are expressed at similar levels in WT T cells (data not shown) indicating that, in a mouse with a polyclonal repertoire, late rearranging Vα genes are well-represented. Spectratype analysis was performed to assess the diversity of CDR3 lengths in expressed TCRα chains in CD8+ splenocytes. This PCR-based assay allows the determination of CDR3 lengths within a specific Vα-Cα pairing and gives a clear indication of the clonality of the population (23). In line with our hypothesis, early TCRα rearrangements generate a Gaussian distribution of CDR3 lengths in both WT and Cre(+) RAG2fl/fl mice, while the CDR3 lengths for late Vα rearrangements are less diverse and less evenly distributed in Cre(+) RAG2fl/fl mice relative to WT (Fig. 4B).

Figure 4
TCR repertoire is skewed to early Vα usage in Cre(+) RAG2fl/fl mice

The phenotype in the GALT is distinct from that in the periphery of Cre(+) RAG2fl/fl mice

In clear contrast to the TCRαβ+ compartment in spleen and LNs, the Thy-1+TCRαβ+ IEL compartment is unimpaired in Cre(+) RAG2fl/fl mice, with no significant difference in the numbers of CD8αβ+, CD8αα+, CD4+CD8αα+ and CD4+ T cells (Fig. 5A and B). Surprisingly, the Thy-1TCRαβ+ IEL compartment is almost completely absent in Cre(+) RAG2fl/fl mice (Fig. 5A and B). In contrast to the secondary lymphoid organs and PPs, the total numbers of IELs are not significantly different between Cre(+) RAG2fl/fl and Cre(−) RAG2fl/fl control mice (Fig. 5C). In the PPs of Cre(+) RAG2fl/fl mice, as in the spleen and LNs, the Thy-1+ compartment is significantly reduced, while the CD19+ B cell compartment appears normal in size (Fig. 5D).Within the Thy-1+TCRαβ+ compartment, the CD4+ population in Cre(+) RAG2fl/fl mice is similar in size to that of Cre(−) RAG2fl/fl controls, while the CD8+ compartment is reduced (Fig. 5E). Thus, in contrast to spleen and LNs, the PP CD4 compartment is less compromised in Cre(+) RAG2fl/fl mice. However, we find similar defects in total cellularity and a reduced TCRαβ+ T cell population in the PPs. Interestingly, the IEL compartment has a distinct phenotype in Cre(+) RAG2fl/fl mice. These data support the notion that Thy-1+TCRαβ+ IELs are the progeny of thymocytes that have undergone early TCR rearrangements, and that Thy-1TCRαβ+ IELs represent a distinct T cell lineage.

Figure 5
The Thy-1TCRαβ+ IEL population is absent in Cre(+) RAG2fl/fl mice

In the presence of competition, bone marrow from Cre(+) RAG2fl/fl mice does not contribute to the peripheral TCRαβ+ T cell population

To determine whether T cells possessing early TCR rearrangements preferentially seed certain peripheral compartments, we made radiation chimeras using a 50:50 mixture of bone marrow from WT (Ly5.1+) and Cre(+) RAG2fl/fl (Ly5.2+) mice to reconstitute lethally irradiated WT hosts (Ly5.1+Ly5.2+). After 8 weeks, the contribution of each donor cell type to the thymus, spleen, LNs and PPs was determined. Cre(+) RAG2fl/fl and WT bone marrow repopulate the DN and DP thymocyte compartments equally well (Fig. 6A). In contrast, the CD4SP and CD8SP populations are comprised almost entirely of cells derived from WT bone marrow. In the spleen, LNs, MLNs and PPs, Cre(+) RAG2fl/fl bone marrow contributes only to the B cell pool and the TCRγδ+, but not TCRαβ+, T cell population (Fig. 6B and C).

Figure 6
Cre(+) RAG2fl/fl bone marrow does not contribute to the peripheral TCRαβ+ T cell population in mixed chimeras

Thymocytes derived from Cre(+) RAG2fl/fl bone marrow are found in the TP population, but do not develop into IELs

Although approximately 30% of the CD8αβ+CD4+CD8αα+ TP thymocyte population is derived from Cre(+) RAG2fl/fl bone marrow (Fig. 7A), TP thymocytes of Cre(+) RAG2fl/fl origin are unable to seed either the Thy-1+ or Thy-1 TCRαβ+ IEL compartments (Fig. 7B and data not shown). In contrast, Cre(+) RAG2fl/fl bone marrow readily contributes to the TCRγδ+ IEL population. Interestingly, while only 3–4% of thymocytes are of host origin, in the IEL population, 40–50% of the TCRαβ+ IELs are derived from the host.

Figure 7
In mixed chimeras, Cre(+) RAG2fl/fl bone marrow contributes to the TP thymocyte population but not to IEL

Discussion

We describe a mouse model in which peripheral T cells are the progeny of thymocytes that have undergone early TCR rearrangements. In these mice, the timing of RAG2 deletion is consistent with previous data showing that in CD4-Cre transgenic mice, Cre expression is initiated at the DN3 stage (15). Phenotypic analysis of the thymus in Cre(+) RAG2fl/fl mice and bone marrow chimeras shows that defects in thymocyte development first become noticeable at the mature DP stage, consistent with a loss of RAG2 in the late DN stage. Consistent with this phenotype, there is a drastic decrease in the CD4SP and CD8SP thymocyte populations. Despite this reduction in thymic output, there are measurable T cell populations in secondary lymphoid organs. Quantitative RT-PCR and spectratype analysis of select Vα genes shows that in Cre(+) RAG2fl/fl mice, deletion of RAG2 effectively limits TCRα rearrangements, maintaining a polyclonal, “early” repertoire in the spleen. Furthermore, the few peripheral T cells in Cre(+) RAG2fl/fl mice have not failed to excise RAG2, as the floxed alleles were excised in 91% and 94% of CD8+ and CD4+ T cells, respectively, as determined by PCR (data not shown). Overall, these data indicate that the majority of T cells in Cre(+) RAG2fl/fl mice bear early-rearranged TCRα chains.

Our data also show that Cre-mediated deletion of RAG2 is specific to T cells and that expression of Cre is not toxic to lymphocytes. B cell numbers are normal in intact Cre(+) RAG2fl/fl mice, and in bone marrow chimeras, Cre(+) RAG2fl/fl and WT bone marrow contribute equally to the B cell population. Despite reduced representation of CD4+ T cells in the spleen and LNs, the phenotype in the GALT suggests that continual Cre expression does not adversely affect cell development or homeostatic proliferation. Additionally, when in competition with WT stem cells, Cre(+) RAG+/+ transgenic bone marrow contributes equally to all T cell populations in mixed bone marrow chimeras (data not shown).

We reasoned that, due to decreased thymic output, the T cell compartment in the secondary lymphoid organs of Cre(+) RAG2fl/fl mice may be expanded by homeostatic proliferation. CD8+ T cells from Cre(+) RAG2fl/fl mice are CD44hiCD62Lhi, while the majority of CD4+ T cells are CD44hiCD62Llo, likely the result of lymphopenia-induced homeostatic proliferation. Indeed, Locksley and colleagues have shown a similar phenotype in their mouse model of lymphopenia, the CD4Cre/R-DTA mouse (24). Furthermore, stimulated CD4+ and CD8+ T cells from Cre(+) RAG2fl/fl mice are functional memory cells, undergoing rapid proliferation and higher levels of IFN-γ secretion compared to WT T cells.

In WT mice, the population of thymocytes undergoing TCRα gene rearrangements before the DP stage is presumably sparse and untrackable. We developed the Cre(+) RAG2fl/fl mouse model to determine whether this early lineage of T cells has the potential to seed certain compartments preferentially. This appears to be the case, as the Thy-1+TCRαβ+ IEL compartment of Cre(+) RAG2fl/fl contains relatively normal numbers, while the Thy-1-TCRαβ+ compartment does not develop. Although the biological function of Thy-1 remains controversial, studies in germ-free mice suggest that Thy-1 expression on at least some subsets of IELs is the result of TCR-mediated activation by external stimuli, most likely intestinal antigens (25). As many of our control and experimental mice are co-housed littermates maintained under specific pathogen free conditions, the difference in IEL Thy-1 expression between Cre(+) RAG2fl/fl and control mice is likely not solely due to antigenic stimuli. However it is possible that the skewed repertoire in Cre(+) RAG2fl/fl mice results in a population of IELs that are more responsive to activation by intestinal antigens. There is also evidence that Thy-1 may be involved in T cell costimulation and homeostasis (26). Thus the lack of a Thy-1 IEL compartment in Cre(+) RAG2fl/fl mice may be due to the need for these cells to undergo lymphopenia-induced homeostasis, leading to the maintenance of Thy-1 cell surface expression. This seems unlikely, given that in athymic nude mice, TCRαβ+CD8αα+ IELs are virtually absent, but nearly all are Thy-1(27). Furthermore, in bone marrow chimeras rendered transiently lymphopenic by irradiation, a substantial Thy-1 IEL population is present from days 1 to 56 post reconstitution (12). In Cre(+) RAG2fl/fl mice, the TCRγδ+ IEL population has a substantial Thy-1 population, suggesting that Thy-1 expression is not an obligate product of the lymphopenic environment in these mice. Instead, it is more likely that the Thy-1 subset represents a separate T cell lineage whose development occurs later or whose function relies on a distinct TCR repertoire. This conclusion is further supported by evidence from LeFrancois and colleagues, who showed that CD8ααTCRαβ Thy-1 and Thy-1+ IELs exhibit differential requirements for IL-15 signaling, including the mechanism of IL-15 delivery (28).

Our data also point to the remarkable homeostatic properties of the IEL population. Despite lymphopenia in secondary lymphoid organs, Cre(+) RAG2fl/fl mice maintain a full IEL compartment. In addition, cells of host origin account for nearly 50% of the IEL pool in bone marrow chimeras, while less than 5% of thymocytes are of host origin. Together, these data suggest that the small populations of IELs and thymic emigrants can fill the available niche in adult lymphopenic mice. It is unclear how these populations would compete for seeding the IEL compartment as it is first established neonatally. The gut environment, where commensal flora provide ample foreign antigen, likely drives this dramatic homeostatic proliferation (29).

Lymphopenia, and the homeostatic proliferation driven by this condition, may lead to autoimmunity in both humans and mice (30). Despite being lymphopenic and possessing a limited TCR repertoire, aged Cre(+) RAG2fl/fl mice do not appear to suffer from systemic autoimmunity. We found no increase in the frequency of anti-nuclear antibodies in the serum of aged (>1 year) Cre(+) RAG2fl/fl mice relative to WT (data not shown). In addition, we found that the percentage of CD4+Foxp3+ T cells in young adult Cre(+) RAG2fl/fl mice was similar to that found in WT mice, except in the spleen, which contained a higher percentage of these regulatory cells (data not shown).

In summary, although comprising a sparse population, T cells bearing the products of early TCR rearrangements can populate the Thy-1+TCRαβ+ IEL compartment and undergo homeostasis-driven proliferation to fill this niche. This property may contribute to the oligoclonal nature of the IEL population and may explain why this oligoclonal repertoire varies, even between genetically identical mice exposed to the same environment (33). Our data suggest that a small number of IEL precursors may efficiently populate the gut, and this property may have also contributed to the historical confusion surrounding the extrathymic versus intrathymic derivation of the IEL compartment. The CD8αα+ IEL subset has several unique properties that suggest a non-conventional pathway of development. These cells appear early in ontogeny, and they have a self-reactive and oligoclonal repertoire (3135). The presence of IELs in neonatally thymectomized and nude mice led to the hypothesis that the thymus may be dispensable for the development of this population. However, recent fate mapping (36) and adoptive transfer (21) experiments have shown that the majority of CD8αα+ IELs are the progeny of DP thymocytes. Development of IELs may continue post-thymically, given that IELs may develop from CD4CD8TCRβ triple negative thymic emigrants in cryptopatches within the gut (37). Our data suggest that the capacity for homeostasis in the IEL compartment can mask defects by driving colonization of the gut by a small number of T cells, whether thymically- or extrathymically-derived.

Acknowledgments

We thank Drs. Klaus Rajewsky, Christopher Wilson, Alexander Rudensky and Hilde Cheroutre for reagents and mice, and Kristina Bavik and Dora Gyarmati for excellent technical assistance and helpful discussion.

Footnotes

1This work was supported by National Institutes of Health Grants AG13078 and AI064318 (P.J.F.) and National Cancer Institute Basic and Cancer Immunology Grant (D.W.H.)

3Abbreviations used in this paper: DN, double negative; DP, double positive; HPRT, hypoxanthine guanine phosphoribosyltransferase; IEL, intraepithelial lymphocyte; LN, lymph node; MLN, mesenteric lymph node; PP, Peyer’s patch; TL, thymic leukemia; WT, wild-type.

References

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