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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2009 November 27.
Published in final edited form as:
PMCID: PMC2709214
NIHMSID: NIHMS121032

Subthreshold glutamate release from mitral cell dendrites

Abstract

The dendrites of a number of neuron types function as presynaptic structures, releasing transmitter following action potentials and dendritic spikes. In this regard, dendrites can function like axons, producing discrete outputs following suprathreshold electrical events. However, as the major site of synaptic inputs, dendrites experience ongoing subthreshold fluctuations in membrane potential, raising the question of whether these subthreshold changes can cause changes in transmitter release. Here we show that mitral cells of the accessory olfactory bulb release glutamate from their dendrites in response to both sub- and suprathreshold stimuli. While subthreshold output was typically low under control conditions, it could be enhanced several fold by pharmacological or endogenous activation of group I metabotropic glutamate receptors (mGluRs). These results indicate that presynaptic dendrites can support two distinct forms of output, and can dynamically regulate how electrical activity is coupled to transmitter release.

Keywords: Vomeronasal System, Accessory Olfactory Bulb, Olfactory Bulb, Phereomone, Olfaction, Odor

Introduction

Neurotransmitter release typically occurs only when regenerative action potentials invade axonal branches. Indeed, subthreshold synaptic inputs are strongly attenuated in axons, and are believed to play only a modulatory role in controlling neuronal output (Alle and Geiger, 2006; Shu et al., 2006). However, the basic rules of excitation-secretion coupling may be considerably different for dendrites that are competent for neurotransmitter release, owing to the close proximity between sites of input and output, and the diversity of electrical events observed in dendrites. Excitable dendrites support a wide range of electrical signals that vary considerably in their amplitude, spatial extent, and time-course (Hausser and Mel, 2003). Thus, both subtheshold electrical activity and subsequent calcium influx may be important triggers of transmitter release in dendrites. Such a diversity of signals would allow a single cell to produce qualitatively different outputs that may serve distinct circuit functions in particular contexts. For example, subthreshold release from dendrites would allow a sensitive local “readout” of membrane potential for small synaptic inputs, while suprathreshold release would occur only for appropriately large or coordinated inputs that evoke regenerative responses.

In the present work we studied the glutamatergic output of dendrites of accessory olfactory bulb (AOB) mitral cells, specifically investigating whether these dendrites support both sub and suprathreshold release. The AOB is an excellent model system for studying properties of dendritic release since the majority of its cell to cell coupling is via dendrodendritic synapses (see schematic in Fig. S1), and specific alterations of dendritic output in this structure are believed to have behavioral consequences (Kaba et al., 1994). We were particularly interested in exploring the possibility of subthreshold release from mitral cells, given our earlier work on how dendritic excitability facilitates the independent integration of synaptic inputs across mitral cell dendritic tufts (Urban and Castro, 2005). These earlier studies pointed to a possible model of pheromone/odor processing in which each dendritic tuft communicates with local interneuron populations via “tuft spikes” - local regenerative events that occur independently of somatic spiking. Sustained subthreshold output from these same neurons could allow for local synaptic communication even when sensory input is weak, possibly extending the dynamic range of pheromone and odor processing.

Here we demonstrate that both sub- and suprathreshold activity in mitral cell dendrites are coupled to dendritic glutamate release, and that both are sufficient to cause firing in postsynaptic interneurons. Moreover, subthreshold dendritic output was graded and persistent, with larger depolarizations evoking higher rates of self-excitation and feedback inhibition that were sustained for seconds. The rate of subthreshold release was typically low under control conditions, but could be enhanced several-fold following either exogenous or endogenous activation of group I metabotropic glutamate receptors (mGluRs). This suggests that not only is there “dual-mode” dendritic transmitter output from mitral cells, but that these neurons can readily alter the relative efficacies of these output modes. We propose that supporting both sub and suprathreshold transmitter release extends the signaling capabilities of single neurons, allowing them to function as versatile computational elements in local microcircuits.

Materials and Methods

Slice preparation

Methods are as described previously (Urban and Castro, 2005). Briefly, parasaggital olfactory bulb slices (300-350 μm thick) were prepared from young mice [postnatal day 14 to 28]. At these ages, the olfactory bulb is fully developed, with mitral cell dendritic morphology at P7 being indistinguishable from adult. Mice were anesthetized (0.1% ketamine/0.1% xylaxine; ~ 3mg/kg, i.p.) and decapitated. Olfactory bulbs were sectioned on a vibratome while submerged in ice-cold oxygenated ACSF solution containing the following (in mM): 125 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 1MgCl2, 25 glucose, 2 CaCl2. In some experiments, 0.5 mM ascorbate, 1mM pyruvate, and 2mM myo-inositol were added to the slicing medium. For imaging of population calcium responses, slices were incubated in a wellplate chamber for 90 minutes at 37°C in a solution containing 500 μL of the ACSF solution described above, with 3μL of 0.01% Pluronic (Molecular Probes), and 10 μM Fura2 acetoxymethyl (AM) ester (Molecular Probes) in 100% DMSO solution. Humidified carbogen (95% O2/5% CO2 was passed above the surface of the liquid in the chamber to keep the solution oxygenated. All animal care was performed in accordance with the guidelines of Institutional Animal Care and Use Committee of Carnegie Mellon University.

Electrophysiology

Whole cell voltage recordings were obtained from the somata of identified AOB mitral cells. Slices were superfused with oxygenated ACSF solution containing the following (in mM): 125 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 1MgCl2, 25 glucose, 2.5 CaCl2, warmed to 34-36° C. In experiments in which recurrent excitation was studied, 0-0.2 mM MgCl2 was substituted for 1.0 MgCl2 in the above solution to facilitate activation of NMDA autoreceptors. Whole-cell recordings were established using pipettes (resistances of 2-8 MΩ) filled with a solution containing the following (in mM): 150 potassium gluconate, 2 KCl, 10 HEPES, 10 sodium phosphocreatine, 4 MgATP, and 0.3 Na3GTP, adjusted to pH 7.3 with KOH. In the indicated experiments, 5mM BAPTA was included in this internal solution. In several experiments, a “high chloride” internal solution was used, in which 152 mM KCl was substituted for potassium gluconate and KCl in the above internal solution. Single cell calcium imaging was performed on cells recorded with electrodes filled with internal solution to which calcium orange (100-200μM); Molecular Probes, Eugene, OR) was added. Voltage clamp and current clamp recordings were made with a MultiClamp 700B amplifier (Molecular Devices, Union City, CA). In experiments in Figure 4 in which we evoked fixed numbers of spikes followed immediately by recordings of subthreshold, voltage dependent currents, we made use of the 700B’s ability to rapidly switch from current to voltage clamp configurations under control of an external trigger. Data were filtered (4 kHz low pass) and digitized at 10 kHz using an ITC-18 (Instrutech, Mineola, NY) controlled by custom software written in Igor Pro (Wavemetrics, Lake Oswego, OR). Fluorescence imaging in single cells (Margrie et al., 2001) was performed using a 16 bit back illuminated frame transfer camera with 10 MHz digitization and 16 μm /pixel (Cascade 512B Roper Scientific, Trenton, NJ) on an Olympus Optical (Melville, NY) BX51WI microscope fitted with a 20X 0.95 NA objective at 20-50 Hz. Calcium Orange, Alexa 594, and Fura2 were visualized using a monochromator for excitation (TILL Photonics, Munich, Germany) and Chroma (Rockingham, VT) CY3 (Calcium Orange and Alexa) and Fura filter sets (excitation filters removed). In all imaging experiments (both single cell and bulk loading), calcium transients were measured as ΔF/F (Urban and Castro, 2005) along lines of interest.

Figure 4
Endogenous glutamate release enhances mGluR-dependent subthreshold release. A, Plot of instantaneous IPSC rates in mitral cells under spontaneous (red) and spike-evoked (gray) conditions (see Materials and Methods). Inset traces show example recordings. ...

Drugs

APV, CNQX, and bicuculline were obtained from Sigma-Aldrich (St. Louis, MO) and used at concentrations of 50, 20, and 10μM, respectively. Gabazine, DHPG and LY367385 were obtained from Tocris (Ellisville, MO), and used at final concentrations of 10, 20 and 100 μM, respectively. 3-((2-Methyl-1,3-thiazol-4-yl)ethynyl) pyridine (MTEP) (Calbiochem, La Jolla, CA) was used at 2μM and prepared from a 25 mM stock in 100% DMSO. LY367585 was prepared in a stock of 100mM in 100% DMSO. The DHPG stock was prepared in deionized H20 (Milli-Q system) at 100 mM.

Data analysis and statistical tests

All data are reported at mean ± SEM. Significance was in all cases assessed by nonparametric statistical tests, as indicated. For the experiments in Figure 2, IPSCs were detected using custom written functions in IGOR pro (Wavemetrics, Lake Oswego, OR), which implemented the event-detecting algorithm described in Kudoh and Taguchi (2002). This analysis resulted in measured rates of spontaneous IPSCs of ~ 1 Hz in control conditions (recording at -55 mV) and this rate was reduced to < 0.2 Hz by blockade of GABAA receptors In certain indicated experiments in which we wanted to compare pre- and post- drug levels of synaptic activity, data were analyzed in the frequency domain. Briefly, single-sided power spectra were obtained using automated routines in IGOR pro, which calculate the squared magnitude of the FFT. Power was quantified by integrating these spectra within frequency bands of interest. Our reasons for analyzing data in the frequency domain were twofold. First, in experiments in which inhibition was blocked and subthreshold, spike independent recurrent excitation was studied, it was difficult to resolve discrete inward currents that could be detected automatically. This is likely due to the fact that glutamate release was tonic and asynchronous, resulting in noisy fluctuations in the current records. Glutamate is also likely to activate predominantly NMDARs, generating relatively slow synaptic currents which are difficult to detect by standard methods. Similar difficulties in the analysis of periods of asynchronous release have been described at other CNS synapses (see, for example, Neher and Sakaba (2001). Second, we wanted to be certain that our counts of synaptic events at various holding potentials were independent of driving force. By calculating power spectra of current records, we were able to compute power ratios of pre- and post- drug conditions, ensuring that the normalized measurements compared across voltages did not reflect differences in driving force, but rather underlying rates of synaptic events. For experiments in which spontaneous and spike-evoked rates of IPSCs were compared (e.g. Figs. Figs.44 and S6), IPSCs were detected using the above described mini-detection algorithm. IPSC raster plots were then constructed in which the peak-event times were counted as single events. These plots (10-20 trials per cell) were pooled across cells in two groups: one corresponding to spontaneous events, and the other to spike-evoked events. From these pooled raster plots, the instantaneous IPSC rates (10 ms bins) vs. time were calculated for the two groups. These plots of IPSC rate vs time were fit using Bayesian Adaptive Regression Splines, which performs spline based generalized nonparametric regression (Behseta et al. (2005); Behseta and Kass (2007)). A likelihood ratio (LR) test was used to compare the two fitted curves pointwise, and significance was assessed for the time dependent quantity -2 log LR, which has a χ2 distribution with 1 degree of freedom (see (Behseta et al., 2007) for details).

Figure 2
Activation of group I mGluRs enhances subthreshold glutamate release from mitral cells. A, Traces show IPSPs superimposed on subthreshold depolarizations evoked by current steps of 220 and 290 pA delivered to a mitral cell. Left, control, arrows indicate ...

Simulation

A simulation was performed in Igor pro (Wavemetrics, Lake Oswego, OR) to confirm that changes in IPSC rate could be reliably detected as changes in the power spectra of current records. Briefly, a poisson process was simulated to construct binary vectors with ones corresponding to IPSC onset times. These vectors were convolved with alpha functions (τ=45 ms) to simulate IPSCs. IPSC amplitudes were chosen from a Gaussian distribution with a mean of 1 and standard deviation of 0.2. A 200 s stimulated IPSC record with λ=20 Hz was chosen as a baseline case, and IPSC records with λ=2 Hz, 5 Hz, and 10 Hz were compared to the 20 Hz record. Power spectra were taken for all records and normalized to the 20 Hz spectrum to observe the approximate frequency band over which differences in IPSC rate were seen.

Results

Sub and suprathreshold activity in mitral cell dendrites evoke glutamate release and activation of postsynaptic interneurons

The primary dendrites of mitral cells of the main and accessory olfactory bulb are “axon-like” in that they can initiate and conduct action potentials in a non-decremental fashion (Bischofberger and Jonas, 1997; Chen et al., 1997; Ma and Lowe, 2004; Urban and Castro, 2005), and contain neurotransmitter filled vesicles. This suggests that these dendrites may make use of classical, axon-like release mechanisms. To test this, we first examined glutamatergic output from AOB mitral cells evoked by backpropagating action potentials. Action potentials (APs) were elicited by whole cell current injection (10 pulses × 3ms at 40 Hz), while calcium transients were monitored in periglomerular (PG) and granule cells that had been loaded with the membrane permeable calcium indicator Fura2-AM. Only PG cells in the vicinity of the tuft of the stimulated mitral cell were activated (Fig. 1A; 4 ± 0.54 PG cells/tuft, avg ΔF/F of 4.0 ± 0.5%, n = 5 mitral cells from 5 animals). Similar activation of local PG cells has been observed in the main olfactory bulb following mitral/tufted cell spiking (Murphy et al., 2005). PG cell calcium transients evoked by mitral cell APs were blocked by APV and CNQX, indicating that PG cell activity was caused by glutamate release from mitral cells (Fig. 1B).

Figure 1
Both sub and suprathreshold stimulation of mitral cells activate postsynaptic cells. A, A1- 20X image of an Alexa 594 filled mitral cell. Scale bar, 50μm. Images beneath the main panel are 3X magnifications of the area demarcated by the white ...

Surprisingly, PG cell calcium transients could also be elicited by subthreshold current or voltage steps in mitral cells (Fig. 1C-F, Fig. S2), suggesting that mitral cells release glutamate during subthreshold depolarization, and that this release is sufficient to activate some local interneurons (median of 2.5 PG cells activated/mitral cell, avg ΔF/F of 0.8% ± 0.2%, n=4 mitral cells from 3 animals). Thus, subthreshold depolarization for 1 second activated approximately 60% as many cells as APs. We next examined whether this activation of interneurons during subthreshold mitral cell depolarization generated recurrent inhibition as is seen following mitral cell spiking. Indeed, we found that subthreshold depolarization of AOB mitral cells in whole cell current clamp recordings caused IPSP-like membrane potential fluctuations (Fig. 2A, n=3). These fluctuations were eliminated following blockade of GABAA receptors by 10 μM gabazine (Fig. 2A) indicating that they were due to recurrent inhibition evoked by subthreshold activation of the recorded mitral cell (see schematic in Fig. S1). To more carefully study these events, mitral cells were voltage clamped at -55 mV and stepped to command potentials between -45 and -25 mV for 1.5 seconds (Figs. 2B & C). Spontaneous synaptic events were rarely observed at -55 mV (~1.0 Hz), but elevated IPSC rates were seen at more depolarized command potentials (7.31 ± 3.18 Hz (n=6) for steps to -25 mV, which was 6.6 fold higher than the rate observed at -55 mV) (p<0.05, Wilcoxon signed rank test) (Fig 2C). IPSCs were observed for the duration of the depolarizing step, and terminated rapidly upon repolarization to -55 mV (Fig. 2B).

Subthreshold release of transmitter from mitral cells is enhanced by activation of mGluRs

Previously we had shown a prominent role for group I mGluR activation in regulating recurrent inhibition elicited by spikes in AOB mitral cells (Castro et al., 2007a). These effects were mediated in part by alterations in granule cell excitability rather than by direct modulation of mitral cell output. Thus, we next examined the role of these mGluRs in regulating subthreshold release. Consistent with our previous observations, addition of the group I mGluR agonist DHPG (20 μM) caused a large and significant increase in the rate of IPSCs or IPSPs observed in mitral cells (Fig. 2A-C). This increase in IPSC frequency was voltage-dependent, and was partially blocked when 5 mM BAPTA was included in the internal solution (Fig. 2C). Thus, DHPG’s action depended in large part on calcium influx into the mitral cell. IPSCs recorded at hyperpolarized potentials were weakly affected by DHPG, but were enhanced dramatically at depolarized potentials (Fig. 2C), with a 30 mV depolarization from -55 mV eliciting IPSCs at a rate of 20.69 ± 3.80 Hz.

A difficulty in interpreting these apparent increases in IPSC frequency is that the driving force on Cl- (and hence GABAA-mediated IPSCs) is larger at more depolarized potentials. Thus the detectability of IPSCs may increase with depolarization, leading to higher apparent event rates. To assess this possibility, we measured the reversal potential of IPSCs in mitral cells and found that spike-evoked IPSCs reverse at -72.42 ± 2.90 mV (n=4), and are clearly visible as outward currents (8.6 pA ± 3.2 pA; n=5 cells) at -60 mV (Fig. S3). This supports the idea that events observed at subthreshold depolarized voltages are evoked, rather than reflecting “unmasked” spontaneous IPSCs.

To more directly measure glutamate release we made use of the fact that glutamate released from mitral cell dendrites “spills over” and binds to NMDA and AMPA receptors located nearby on the same dendrite (Nicoll and Jahr, 1982; Isaacson, 1999; Margrie et al., 2001; Urban and Sakmann, 2002). First, we blocked inhibition with 10 μM gabazine and bathed slices in a 0 Mg2+ ACSF solution to enhance NMDAR-mediated currents. We then depolarized the mitral cell in voltage clamp and recorded spill-over mediated glutamate currents, which were stationary for the duration of voltage steps (Fig. S5). Under these conditions depolarization will decrease driving force on glutamatergic currents, reducing detectability of events, resulting in an underestimate of the voltage dependence of release (events observed at -25 mV, for example should be ~ 45% the amplitude of events observed at -55 mV, assuming a reversal potential of 0 mV). In these experiments, noisy barrages of inward synaptic currents were evoked by subthreshold depolarization from -55 mV to command potentials as low as -45 mV (Fig. S4). These fluctuations were blocked by addition of the glutamate receptor antagonists APV (50μM), CNQX (20μM), and MK801 (30μM), indicating that they were glutamate-evoked synaptic currents. Because discrete events were often difficult to discern in these traces, we quantified self-excitatory currents by calculating the RMS value of membrane current. The magnitude of these glutamatergic currents increased with voltage (Fig. S4D), despite a less favorable driving force at depolarized voltages. Thus, the enhanced rate of recurrent IPSCs we observed upon mitral cell depolarization (Fig. 2) is at least partly due to glutamate output at subthreshold voltages.

We next turned to the enhancement of asynchronous IPSCs by group I mGluR activation that we observed above. This effect may be due to enhanced glutamate release from the mitral cell dendrites (as suggested by the fact that it is blocked by intracellular calcium buffering in the mitral cell) but it also may in part reflect mGluR-mediated increased interneuron excitability (Castro et al., 2007). To test these alternative explanations, we again measured self-excitation of mitral cells and observed the effect of DHPG on self-excitatory currents. We examined self-excitation evoked by subthreshold depolarizations of mitral cells following blockade of inhibition by 10 μM gabazine (in 0.2 mM Mg2+ ACSF), allowing us to assess glutamate release directly from the recorded mitral cell. To account for the possibility that DHPG may cause a nonspecific increase in ambient glutamate (for example via glutamate spillover from neighboring mitral cells), we calculated the ratio of EPSC power during depolarization to EPSC power during resting potential. In mitral cells clamped at -55 mV and stepped to depolarized potentials, DHPG caused enhanced rates of EPSCs and glutamate receptor dependent fluctuations in synaptic current at depolarized potentials (Fig. 2D & E). Thus, activation of group I mGluRs enhances subthreshold release of glutamate by AOB mitral cells.

Dendritic calcium influx evoked by sub and suprathreshold dendritic activity

Given the nonlinear relationship between calcium influx and transmitter release described at most synapses (Dodge, Jr. and Rahamimoff, 1967; Bollmann et al., 2000; Schneggenburger and Neher, 2000), and the observed blockade of subthreshold release by buffering intracellular calcium (Fig. 2C), we reasoned that subthreshold depolarization should cause sustained calcium transients in mitral cell dendrites. We thus first compared dendritic calcium transients evoked by single backpropagating spikes and subthreshold current injection. Both of these stimuli evoked relatively uniform calcium elevations along mitral cell primary dendrites (Fig. 3A). Interestingly, subthreshold calcium transients were visible even in distal tufts in response to subthreshold current injection or voltage clamp depolarizations (Fig. 3A & B). Subthreshold transients evoked by current injection were uniformly smaller than single-AP associated transients (16.2 ± 3.3%; n=5 cells), and showed slow rising phases (552 ± 88 ms to peak; n=5 cells). These transients were substantially attenuated by 100 μM Ni2+ (amplitudes post nickel were 28 ± 10% control; n = 5) and in voltage clamp experiments, calcium traneisnts evoked by subthreshold depolarization were enhanced by hyperpolarizing pre-pulses to -80 mV for 500 ms (calcium transients were 128 ± 11% of control P<0.01 n=6 cells) Together, these data indicate that LVA calcium channels carried the majority of subthreshold calcium current. Somewhat surprisingly, dendritic calcium transients evoked by subthreshold somatic current or voltage steps were unchanged by DHPG (Fig. 3C1 & C2). For depolarizations to -25 mV, for example, control transients were 25.1 ± 10.2% the amplitude of single BAP-evoked transients, while transients following DHPG addition were 26.1 ± 7.7% the amplitude of BAP-evoked transients (p=0.59, Wilcoxon signed rank test, n=5); peak calcium influx in response to single BAPs was not altered appreciably by DHPG (pre: 18.0 ± 1.8% ΔF/F; post: 18.9 ± 1.0 % ΔF/F; p=0.43, Wilcoxon signed rank test, n=6), allowing us to normalize subthreshold transient amplitudes for comparisons across cells. This general lack of effect of DHPG on subthreshold calcium transients can also be seen directly in the ΔF/F transients shown in figure 3C1. Similarly, resting calcium levels in mitral cell dendrites were unchanged by DHPG (resting fluorescence post DHPG = 101 ± 2% control values, n = 5 cells, p>0.5 for control vs. DHPG).

Figure 3
Mitral cell dendritic calcium transients associated with subthreshold depolarization and backpropagating spikes. A, A1 - fluorescence image of a mitral cell (tuft is toward top right) with line of interest (LOI) shown along the principal dendrite. A2 ...

In total, these data suggest that activation of group I mGluRs enhances the coupling between calcium influx and transmitter release without directly altering calcium influx through channels mediating subthreshold release. Consistent with this “priming” effect of mGluRs on transmitter release, we also observed that release is enhanced for suprathreshold stimuli. In experiments in which we recorded spikeevoked recurrent EPSPs (rEPSPs) in mitral cells, DHPG (20 mM) increased rEPSP amplitude from 2.46 ± 1.02 mV to 4.43 ± 0.97 mV; n=5; p<.03, Fig. S7)).

Endogenous activation of mGluRs is sufficient to enhance subthreshold dendritic transmitter release

We next tested whether the mGluR-dependent enhancement of subthreshold release occurs following physiological release of glutamate. To test this, we evoked action potentials (7 pulses × 3ms duration at 40Hz) in mitral cells in current clamp, and rapidly (within a few ms after the last AP) switched to voltage clamp to hold cells at potentials of either -45 or -35 mV in interleaved trials (Fig. 4, see also Materials and Methods). This allowed us to evoke a specific number of mitral cell APs, which would cause glutamate release and subsequent mGluR activation, and then examine the voltage dependent, subthreshold component of recurrent inhibition in voltage clamp. Since the number of spikes was the same across trials, the rate of action potential evoked recurrent IPSCs should be identical for the two command potentials. Thus, differences in IPSC rate observed at the two potentials will be a function of voltage command potential following the spikes. To ensure that the IPSCs studied were not AP evoked, we only examined IPSCs occurring in a 1 second long interval beginning 500 ms after the final spike (In a related set of experiments, we determined the duration of AP-evoked recurrent inhibition to be < 500 ms (Figs. (Figs.4A,4A, and S6)). Under control conditions, IPSC-associated power was approximately 3 fold greater at -35 mV than at -45 mV (Fig. 4E), comparable to the fold difference we observe between these two potentials following addition of DHPG (Fig. 2). Addition of the mGluR1 and mGluR5 antagonists LY367385 (100 μM) and MTEP (2 μM) eliminated the difference in IPSC-associate power observed at-35 vs. -45 mV (Fig. 4E), demonstrating that mGluR activation by self excitation is sufficient to enhance the subthreshold component of release. The most relevant comparisons are between control and drug conditions within each voltage condition, as these demonstrate that reduction of IPSC power by LY + MTEP is selective for more depolarized holding potentials (see also Figure S8).

Discussion

We have shown that both sub and suprathreshold depolarization cause release from dendrites of AOB mitral cells. Both forms of dendritic release can elicit spiking in postsynaptic interneurons, though suprathreshold release typically activated more cells than subthreshold release. Subthreshold glutamate release and concomitant recurrent inhibition were low under control conditions (IPSCs occurred at ~ 7 Hz for depolarizations to -25 mV), but could be enhanced up to six fold by pharmacological or physiological activation of group I metabotropic glutamate receptors. Activation of endogenous receptors by trains of action potentials in single mitral cells resulted in an approximate doubling of subthreshold release. These results indicate that mitral cells support two qualitatively different output modes whose relative efficacy can be changed by activation of neuromodulatory systems.

Functions of subthreshold release

Since the first descriptions of dendrites as presynaptic elements, there has been great interest in understanding the coupling of electrical signals to dendritic transmitter output (Margrie and Urban, 2008; Egger and Urban, 2006). This interest originally derived from the proximity of sites of synaptic input and output, and was enhanced by the observation that dendrites express voltage-dependent channels and exhibit regenerative activity at multiple spatio-temporal scales. Recent work by Acuna-Goycolea et al (2008) underscores this point by demonstrating that GABA release from thalamic interneurons occurs for both plateau-like dendritic calcium spikes, as well as rapid, full-blown sodium spikes. Interestingly, these distinct regenerative events also evoke GABA release over distinct timescales. Similarly, synapse specificity of cannabinoid release from Purkinje cell dendrites can be tuned by the spatial extent of local dendritic spikes (Rancz and Hausser, 2006).

Here we show that mitral cell dendrites release transmitter upon subthreshold depolarization, in the absence of sodium spikes or other forms of regenerative activity. This suggests that the olfactory bulb makes use of a “hybrid” neural code in which sensory information is encoded by the rate and/or timing of mitral cell spiking, as well as by the membrane potential of sufficiently activated mitral cells. Thus, bulbar circuit operations such as lateral inhibition and self-excitation/inhibition may occur among mitral cells receiving sensory inputs, but which do not send action potentials out of the bulb. One consequence of this is that local circuits consisting of mitral cells and interneurons that receive only weak sensory inputs could still participate in local computations that would ultimately contribute to the “decision” of mitral cells to spike or not. Since mitral cells receive direct inputs from sensory neurons and send their axons out of the bulb, such local processing may be critical for generating selective output and controlling whether spikes are fired by mitral cells.

In situations in which many glomeruli are activated by a multi-component odor or pheromone mixture, for example, outputs from mitral cells responding to “trace” quantities of an odorant could modulate the firing of postsynaptic neurons. Subthreshold transmitter release may be especially important in systems like the AOB, where the firing of only a few principal neurons is believed to be behaviorally relevant (Luo et al., 2003). Because spiking is costly in these same systems if it occurs for inappropriate stimuli, regulatory feedback occuring during subthreshold integration may provide a “safety check” on spike generation.

Regulation of neuronal output by subthreshold membrane potential

Several recent studies have shown that subthreshold membrane voltage can influence action potential evoked transmitter release from axons of CNS neurons (Awatramani et al., 2005; Shu et al., 2006; Alle and Geiger, 2006). In some of these cases, subthreshold depolarization causes elevations of calcium that are sufficient to potentiate release. These “priming” effects on release will be especially pronounced in release-competent dendrites, given the close proximity of sites of synaptic input and output: in essence, dendritic release sites are more likely to experience large subthreshold depolarizations (and subsequent elevations of calcium) than their axonal counterparts. Evidently, these elevations of basal calcium concentration can also elicit dendritic release without APs.

This observation builds on related studies describing other “nontraditional” forms of transmitter release that occur in the absence of action potentials or obvious regenerative activity. Chavez et al (2006) have recently shown that activation of calcium permeable AMPA receptors alone can directly trigger GABA release from A17 Amacrine cells. Similarly, calcium influx through NMDA receptors may be sufficient to directly evoke GABA release from olfactory bulb granule cells (Chen et al., 2000).

MGluR modulation of release

One of the striking attributes of the subthreshold release we studied was its enhancement by activation of group I metabotropic glutamate receptors. While presynaptic mGluRs modulate release in many cases by altering calcium currents (Takahashi et al., 1996), we observed no change in the amplitude of depolarization-evoked calcium transients in mitral cell dendrites in the presence of group I mGluR agonists. This suggests that mGluRs have modulatory actions on release machinery downstream of calcium entry. Such effects have been described in several preparations. In CA3 Pyramidal cells, for example, the frequency of mEPSCs is reduced by mGluR agonists even when voltage gated calcium channels are blocked (Scanziani et al., 1995). Mechanistically, such “late-step” changes in release may involve alterations in the calcium cooperativity and/or sensitivity of vesicle exocytosis initiated by second messenger cascades. Our data directly address this issue in the AOB, but given the similarity of mGluR effects seen in AOB and MOB (Dong et al., 2007; Castro et al., 2007b) similar facilitation of release may occur in MOB mitral cells as well.

Many studies of such active dendritic integration emphasize the role of regenerative conductances, which can shape the amplitude and time-course of synaptic inputs. Although suchregenerative activity allows subtle computations to be performed by single neurons, most models of neuronal function presume that neurons are feedfoward devices that act in relative isolation while integrating their inputs. By contrast, our discovery of subthreshold dendritic release indicates that single neurons can produce outputs in the absence of spikes, which in turn allows feedback via local circuits to occur during integration.

Supplementary Material

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Acknowledgements

The authors thank Greg Larocca for technical assistance.

Funding

This work was supported by the National Institute on Deafness and Other Communication Disorders Grant RO1 DC005798 (NNU), and a National Research Service Award predoctoral fellowship (F31 DC08230 (JBC)).

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