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Ann Bot. 2009 May; 103(7): 1039–1048.
Published online 2009 March 2. doi:  10.1093/aob/mcp045
PMCID: PMC2707916

Chromosome elimination, addition and introgression in intertribal partial hybrids between Brassica rapa and Isatis indigotica

Abstract

Background and Aims

Partial hybrids with female-parent-type phenotypes and chromosome numbers but altered genomic compositions have been reported in wide crosses of several plants. In order to introgress desirable genes from a wild relative, Isatis indigotica (a dye and medicinal plant; 2n = 14), into Brassica crops, intertribal sexual hybridizations were carried out with B. rapa (2n = 20), and the resulting hybrids and their progenies were characterized.

Methods

Using genomic in situ hybridization (GISH) and amplified fragment length polymorphism (AFLP), chromosomal/genomic components of the hybrids and their progenies were analysed.

Key Results

Many hybrid plants were obtained from the mature seeds harvested from the B. rapa × I. indigotica cross, and these exhibited different morphological traits. However, the majority of them did not survive and only three plants grew to maturity. These three hybrids showed poor growth and much smaller stature than the two parents, but had some morphological traits and chemical composition of I. indigotica. One plant had 2n = 10, the haploid chromosome number of B. rapa, and was absolutely sterile. The other two plants had 20 and 22 somatic chromosomes and were male sterile but produced seeds following pollinations with B. rapa. All back-cross progenies over several generations maintained a B. rapa-type phenotype and also displayed some variations in morphological characters and fatty acid compositions. They were all 2n = 20 and showed good seed-set. The hybrid with 2n = 22 produced some progeny plants with 2n = 21 and 2n = 22. GISH detected two chromosomes of I. indigotica in the hybrid with 2n = 22 but none in the one with 2n = 20. AFLP bands specific for I. indigotica, novel for two parents or absent in B. rapa, were detected in the two hybrids and their progenies. These progeny plants were novel B. rapa types with an altered genomic constitution or alien additions.

Conclusions

Complete or partial chromosome elimination and diploidization with genomic rearrangements were considered to lead to the formation of partial hybrids in this cross.

Key words: Brassica rapa, Isatis indigotica, intertribal hybridization, partial hybrids, chromosome elimination, alien addition, introgression, genomic in situ hybridization (GISH), amplified fragments length polymorphism (AFLP)

INTRODUCTION

Currently there is much interest in hybridization in evolutionary biology. Since it was first emphasized as an evolutionary force by Lotsy (1916), it has been recognized that hybridization and introgression are of wide occurrence among natural plant populations (Anderson, 1949; Anderson and Stebbins, 1954; Grant, 1981; Arnold, 1992, 2004; Dowling and Secor, 1997). Hybridization has been reported to contribute to speciation in three ways: allopolyploidy from the duplication of chromosome complement in a hybrid (Otto and Whitton, 2000); homoploid hybrid and recombinational speciation (Rieseberg and Wills, 2007); and introgression from back-crossing of the hybrids to parents (Rieseberg and Carney, 1998). The importance of hybridization for speciation has been emphasized in recent years (Rieseberg et al., 2003; Cronn and Wendel, 2004; Baack and Rieseberg, 2007; Hegarty and Hiscock, 2008;). Empirical studies of hybrid speciation using molecular genetic tools over the past decade (Rieseberg et al., 1995; Arnold, 1997, 2004; Wendel, 2000; Cronn and Wendel, 2003; Rieseberg et al., 2003; Doyle et al., 2004; Seehausen, 2004) suggest that interspecific hybridization may be a more powerful and creative evolutionary process than envisioned by the early proponents of hybridization (Cronn and Wendel, 2003), such as Anderson (1949), Stebbins (1950) and Grant (1981). Although introgressive hybridization does not necessarily lead to immediate hybrid speciation, it is an important mechanism for the transfer and/or de novo origin of traits related to ecological adaptations and therefore plays an important role in facilitating speciation in changing niches (Arnold, 2004). There is also much debate about the significance of wide hybridization (Chapman and Burke, 2007; Buggs et al., 2008).

Gene transfer via wide hybridization still remains a potent approach to broaden gene pools and increase genetic variability in breeding programmes and genetic research aimed at creating novel plant forms and improving the quality of crops. Related or distant species of cultivated crops constitute a large reservoir of genes conferring desirable traits, such as resistance to biotic or abiotic stresses. The genus Brassica contains several species that are major sources of edible oil and vegetables. These have been hybridized with wild and weedy species of primary, secondary and even tertiary gene pools to synthesize entirely new hybrids, and there are many examples of successfully transferring alien genes to them (Prakash et al., 2009).

Partial hybrids having a phenotype and chromosome number similar to their female parents and carrying some DNA sequences specific for the male parent, or novel for the two parents but absent in the female parent, have been reported in coffee (Lashermes et al., 2000), rapeseed (Cheng et al., 2002; Hua et al., 2006; Chen et al., 2007; Liu and Li, 2007; Du et al., 2008), rice (Liu et al., 1999) and sunflower (Faure et al., 2002a, b). Several examples of intertribal sexual hybrids involving Brassica species have been reported that are partial hybrids with or without alien chromosomes or chromosomal segments but with altered genomic compositions: between B. napus, B. carinata, B. rapa and Orychophragmus violaceus (Cheng et al., 2002; Hua et al., 2006; Liu and Li, 2007); between B. napus and Matthiola incana (Luo et al., 2003); between B. napus, B. rapa and Capsella bursa-pastoris (Chen et al., 2007); and between B. napus and Lesquerella fendleri (Du et al., 2008). Of these wild species, Orychophragmus violaceus is phylogenetically placed outside the Brassiceae tribe by comparative chromosome painting (Lysak et al., 2007). The possible mechanisms in these examples involve the early elimination of alien fragments or chromosomes of the male parent, genome rearrangements consecutive to ‘genomic shock’ (McClintock, 1984; Madlung and Comai, 2004) and diploidization (Faure et al., 2002a; Liu and Li, 2007).

Isatis indigotica (Chinese woad or tein-cheing, 2n = 14, II), belonging to the Isatideae tribe (Al-Shehbaz et al., 2006) of the Brassicaceae family, is a dye crop containing the same indigo precursors indican (indoxyl b-D glucoside) and isatan B (indoxyl ketogluconate) in its leaves as those of I. tinctoria L. (woad) but with a greater indigo-production capacity (Angelini et al., 2007). As a traditional Chinese medicine, I. indigotica contains compounds that may cure many diseases caused by bacteria and viruses and increase the protective immune response (Chen et al., 2005). These compounds may be very beneficial for improving the quality of Brassica vegetable crops. Additionally, I. indigotica also shows resistance to tobacco mosaic virus (TMV; Wang and Wan, 1988) and stem rot (Sclerotinia sclerotiorum), the most serious disease of rapeseed in China (Zhao et al., 1994). Intertribal somatic hybrids between B. rapa spp. chinensis and I. indigotica have been synthesized by symmetric protoplast fusion and characterized (Tu et al., 2008). The purpose of the present study was to transfer genes conferring desirable traits from I. indigotica to B. rapa through sexual hybridization. Sexual hybrids with I. indigotica as the male parent were produced by hand emasculation and pollination and their chromosomal/genomic compositions analysed using the methods of genomic in situ hybridization (GISH) and amplified fragment length polymorphism (AFLP). The results indicate chromosome elimination, addition and introgression leading to the production of partial hybrids and their back-crossing progenies, with some characters from I. indigotica and an altered genomic constitution of B. rapa.

MATERIALS AND METHODS

Plant material and crosses

The two parents used for crosses were Brassica rapa L. subsp. chinensis (L.) P.Hanelt ‘Aijuehuang’ (2n = 20, AA) and Isatis indigotica Fortune ex Lindl. (2n = 14, II). In order to control soil water and to shelter plants from the high air humidity during the winter and early spring in Wuhan, where the experiment was conducted, I. indigotica was planted in a glasshouse as its growth is retarded under humid conditions. Crosses were made by hand emasculation and pollination with I. indigotica as the pollen parent on sunny days and mature seeds were harvested for study. The seeds were placed on wet filter paper in a Petri dish for germination and seedlings were planted in small plastic cups filled with soil, then the young plants were transplanted into the field. The hybrid plants identified were maintained on MS medium (Murashige and Skoog, 1962) by culturing the auxiliary buds for further study in the next generation.

Cytology and pollen viability

The ovaries from young flower buds were used to determine the chromosome numbers of the hybrids. After pre-treatment with 8-hydroxyquinoline for 3–4 h at 22 °C, the ovaries were fixed in an ethanol/acetic acid solution (3:1), and stored at −20 °C. The flower buds were fixed directly in 3:1 ethanol/acetic acid and then stored at 4 °C for meiotic analysis. Determination of chromosome numbers and meiotic observations were made according to the method of Li et al. (1995). Fertility based on pollen viability was determined as the percentage of pollen grains stained with aceto-carmine (1 %).

Probe labelling and GISH analysis

Total genomic DNA from young leaves was extracted and purified according to the method of Dellaporta et al. (1983). DNA of I. indigotica was labelled with bio-11-dUTP (Sino-American Biotechnology Company, China) by the nick-translation method and used as a probe; the length of the probe DNA fragments averaged approx. 500 bp. DNA of B. rapa subsp. chinensis ‘Aijuehuang’ was sheared to 300–500-bp fragments by boiling for 15 min and used as the block. The content of probe and blocking DNA in the hybridization mixture was 3 µg mL−1 and 20 µg mL−1, respectively. Slide preparations of chromosomes for GISH mainly followed the procedures of Zhong et al. (1996), and GISH was carried out according to Tu et al. (2008).

AFLP analysis

Amplified fragment length polymorphism analysis was performed following the protocol of Vos et al. (1995). Genomic DNA from young leaves of the hybrids, parents and progeny was entirely restricted by EcoRI and MseI, then EcoRI and MseI adapters were ligated to the restriction fragment ends. After two steps of PCR (preselective PCR and selective PCR, in turn), the PCR products were loaded on the gel and resolved. Finally, the AFLP profile was obtained by silver staining (Bassam et al., 1991) and the bands of 80–800 bp were scored. Twenty-one pairs of primers were randomly selected and used for AFLP fingerprint analyses of the hybrids, and ten or 12 pairs for back-cross progeny.

Fatty acid analysis, and diethyl ether and ethanol extraction of leaves

Fatty acids of the seed oil were analysed using a gas chromatography instrument (HP 6890, Germany) following the procedures of Chen et al. (2007).

The basal leaves (1·0 g) from two parents and hybrids were immersed in 4 mL diethyl ether for 4 h, then removed and dried in air; the extraction solutions were retained for observation. The dry leaves were then immersed in 4 mL of 95 % ethanol for 4 h and the extracts were used for study (Sun et al., 2007).

RESULTS

Production and morphology of hybrids and progenies

With I. indigotica as the male parent, the majority of seeds harvested from the female parent B. rapa germinated and produced seedlings and young plants. At the seedling stage, 14 of the F1 plants were morphologically different from the female parent, B. rapa (Fig. 1A11), and expressed some characters of I. indigotica, such as short stature and spatulate leaves (Fig. 1A-2, A-3). Some seedlings had three cotyledons (Fig. 1A-4). The rest were very similar to B. rapa. After transplantation into the field, only three of the 14 plants (designated H1–H3) grew to maturity; the others did not survive, including those with three cotyledons. The plants similar to B. rapa developed well and showed high pollen stainability (>90 %) and normal seed-set, and consequently no further studies were made on them.

Fig. 1.
Phenotypes of hybrids between B. rapa and I. indigotica. (A) Seedlings of B. rapa subsp. chinensis: (A-1) ‘Aijuehuang’, (A-2) plant H1, (A-3) plant H2, and (A-4) the seedling with three cotyledons. Scale bars = 5 cm. (B) Young plants of ...

All three hybrids (H1–H3) were poor in growth with smaller stature than the two parents. However, they expressed some traits of I. indigotica, such as spatulate and dark-green leaves, basal clustering branches and small petals. The H1 plant, which showed poor growth during the whole growth period, had spatulate leaves (Fig. 1B-2, C) and was green, as in B. rapa (Fig. 1B-1). The H2 plant (Fig. 1B-3) grew more slowly than H1, and also had spatulate, but dark-green, leaves (Fig. 1B-3, E), as in I. indigotica (Fig. 1B-4); its leaves were also smaller than those of H1. The colour of the diethyl ether extract of the leaves of the two plants was nearly the same as that of the two parents; however, the colour of the ethanol extraction of I. indigotica was of a dark hue and that of B. rapa was jade-green, while H1 and H2 exhibited an intermediate colour between the two parents (Fig. 2) and the colour of H2 was of a darker hue than that of H1. The H2 plant flowered earlier than H1 and the two parents (Fig. 1B-3), and the flowers of these two plants were intermediate in size between the two parents (Fig. 1D, F), with crimpy petals. The plants had many basal branches (Fig. 1G-1), similar to I. indigotica (Fig. 1G-2). Both plants were male sterile with rudimentary stamens and bulky and flexible pistils, but produced seeds normally when pollinated with B. rapa. The H3 plant resembled H1 and H2 morphologically, but grew much more weakly. It was male sterile and produced no seeds after pollination with B. rapa.

Fig. 2.
Ethanol extracts from leaves of hybrids between B. rapa and I. indigotica: I., I. Indigotica; B., B. rapa; H1, H2 are two hybrids.

The back-cross seeds from H1 germinated well and seedlings grew vigorously into mature plants in the field. Although 54 BC-F1 plants were morphologically inclined towards the female parent, B. rapa, they showed some differences in leaf colour, flower colour (primrose or deep yellow) and size of flowers. The pollen viability ranged from 54·1 to 99·6 %, and averaged 91·5 %. BC-F1 plants were selfed three times and the progenies in each generation showed segregations for some characters. Most of them had normal growth and fertility, but a few showed poor growth, spatulate leaves, small petals (Fig. 1H) and total sterility, as for those of the F1 hybrid plant.

Due to poor growth, the plant H2 failed to produce seeds when pollinated by B. rapa. The regenerated plants from its cultured buds showed the same morphology as the seeded one and also the weak growth, and produced no back-cross seeds in the first year, but did in the second year. Twenty-one BC-F1 plants were raised, which expressed morphological characters mainly of the female parent, B. rapa. They had high pollen viability (>90 %, except for two plants with approx. 80 %) and good seed-set after selfing.

Cytogenetic and GISH analyses of hybrids and progenies

When the I. indigotica probe was applied to the preparations of B. rapa ‘Aijuehuang’ (the DNA of itself as the block), signals of large size and strong intensity were mainly located at the two terminals of one V-shaped bivalent or the centromeric part of another bivalent at diakinesis, or at the terminal or centromeric parts of two chromosomes in each polar group of pollen mother cells (PMCs) at anaphase I. This made it easier to distinguish parental chromosomes/chromosomal segments in these hybrids.

H1 had 2n = 20 in ovary cells and PMCs, the same as the female parent, B. rapa. PMCs showed normal chromosome pairing with ten bivalents (II) at diakinesis and segregation of 10:10 at anaphase I (A-I), but one lagged chromosome appeared in 2·3 % of A-I PMCs (Table 1). GISH with the I. indigotica probe detected no obvious signals on chromosomes of PMCs, except for strong signals on two chromosomal terminals of one bivalent (Fig. 3A), and on the terminal or central part of one chromosome in each polar group (Fig. 3B), which was nearly the same as in B. rapa and probably resulted from the cross-hybridization of repetitive DNA, such as rDNA. All 54 BC-F1 plants had 2n = 20 in their ovary cells and produced PMCs with ten bivalents at diakinesis and segregation of 10:10 at A-I, but also occasionally with one laggard chromosome (Table 1).

Fig. 3.
Cytology of hybrids between B. rapa and I. indigotica, and progenies. DAPI (blue) and merged images of each pollen mother cell (PMC) are shown (red signals from the I. indigotica probe). The labelled chromosome fragments and chromosomes are indicated ...
Table 1.
Cytology of hybrids and back-crossing progenies from a B. rapa × I. indigotica cross

H2 possessed 2n = 22 in ovary cells and produced PMCs mainly with 11 bivalents at diakinesis. Chromosomes segregated mainly as 11:11 (43·2 %; Fig. 3C) and 10:12 (45·4 %; Fig. 3E) in A-I PMCs, but 9:13 and other ratios with one or two laggards at lower frequency (11·4 %; Table 1). The segregation of 22:22 also appeared occasionally, which was probably the result of precocious division of the chromosomes. Meiotic irregularities occurred with frequent anaphase bridges and lagging chromosomes. Two entirely or partly labelled chromosomes were observed in A-I PMCs with the segregations of 11:11 or other ratios, which were equally or unequally distributed in each polar group (Fig. 3D–F). The unlabeled laggards also appeared in some A-I PMCs (Fig. 3F).

Among the 21 BC-F1 plants from H2, eight had 2n = 20, nine had 2n = 21 and four had 2n = 22 chromosomes. These plants did not show major differences in morphological characters and were shorter in height than B. rapa. Plants with 2n = 20 produced PMCs with ten bivalents at diakinesis and A-I segregation of 10:10 (95·2 %), and also occasionally with one laggard chromosome (4·9 %; Table 1). Their pollen viability ranged from 76·2 to 98·4 %, and averaged 92·3 %. Plants with 2n = 21 (Fig. 3G) had PMCs with ten bivalents and one univalent at diakinesis (Fig. 3H) and A-I segregation of 10:11 (Fig. 3I). One laggard chromosome was usually observed at metaphase I (Fig. 3J). The percentages of segregation of 10:11 and 10:10:1 (laggard chromosome) at A-I were 81·3 and 18·7 %, respectively (Table 1). Their pollen viability was high (92·8–96·8 %), and averaged 94·6 %. Plants with 2n = 22 (Fig. 3K) formed 11 bivalents at diakinesis (Fig. 3L) and had A-I segregations of 11:11 (43·7 %; Fig. 3M), 10:12 (46·6 %; Fig. 3N), and others: 9:13, 10:10:2 and 10:11:1, (9·8 %; Table 1). Their pollen viability was 79·4 to 93·3 %, with an average of 88·7 %. It was notable that the laggard in Fig. 3J, the univalent in H, and the one bivalent arrowed in Fig. 3L were small sized. Chromosomes of I. indigotica were observed to be smaller as compared to B. rapa in their somatic hybrids (Tu et al., 2008).

H3 had 2n = 10 in ovary cells, the haploid number of B. rapa. Ten univalents were seen in most of the diakinesis PMCs. A-I PMCs showed segregations of 5:5 and 6:4 predominantly, and 7:3, 8:2 and 9:1 at low frequency.

AFLP analyses of hybrids and progenies

Among the polymorphic bands amplified (Fig. 4, Table 2), those specific for I. indigotica were present at a lower frequency in H1 (12·3 %) than in H2 (17·4 %), but those absent in B. rapa had higher frequency in H1 (22·5 %) than in H2 (16·7 %), while those novel for the two parents were comparable (9·9 % for H1, 10·6 % for H2). Therefore, there was a positive association between morphology (Figs 1, 2), cytology (Fig. 3) and genomic composition (Fig. 4, Table 2) in these two hybrids. All BC-F1 plants from H1 and H2 also possessed the three kinds of AFLP bands (Fig. 4), and those from H2 had higher means of specific bands and also novel or absent bands (Table 3). On average, all BC-F1 plants had fewer specific bands than the two hybrids, but more novel and absent bands, except the novel bands in those plants from H1 (Table 3).

Fig. 4.
Representative AFLP profiles for hybrids between B. rapa and I. indigotica, and progenies. (A) Hybrids; and BC-F1 plants from (B) H1 and (C) H2. I., I. indigotica; B., B. rapa; M, DNA marker. Black arrows show the I. indigotica-specific bands in the three ...
Table 2.
Numbers and percentages of AFLP bands in hybrids from a B. rapa × I. indigotica cros:s
Table 3.
Percentages of AFLP bands in BC-F1 plants from a B. rapa × I. indigotica cross

Fatty acid composition of progenies

In the seed oil of 30 randomly selected BC-F5 plants from H1, the contents of seven fatty acids had obvious changes as compared to the parent B. rapa (data not shown). Most plants had increased linolenic acid and reduced erucic acid contents. The seed oil of 18 BC-F1 plants from H2 contained variable contents of seven fatty acids. The levels of linolenic and eicosenoic acids were generally higher, but that of erucic acid lower than in B. rapa (data not shown).

DISCUSSION

Due to very distant relationships, which cause strong reproductive barriers and/or pre- and post-zygotic sexual incompatibilities, sexual intertribal hybridizations between Brassica crops and the species of other tribes have not frequently been reported (Luo et al., 2003; Chen et al., 2007; Du et al., 2008; Prakash et al., 2009). In the present study, progenies from sexual crosses of B. rapa × I. indigotica were derived from mature seeds after repeated hand emasculations and pollinations. Characterization of morphology, cytology and genomic composition confirmed hybridity in these crosses and the progeny plants. However, the derived progeny plants were not complete Mendelian hybrids carrying the summation of parental genomes, but were partial hybrids mainly with chromosomal/genomic constituents from the female parent. It is notable that the majority of young F1 plants that differed in phenotype from B. rapa failed to survive to maturity. The three plants that flowered showed retarded growth and much smaller stature than the two parents (Fig. 1). Despite this, they maintained the intact genome of B. rapa, with (plant H2) or without (H1) individual I. indigotica chromosomes. This suggests that the normal growth in these progeny plants is disturbed by the introgression of alien DNA segments or genetic rearrangements (Fig. 3; Table 2), and also that the diploid B. rapa buffers the genetic changes less than the allotetraploid B. napus (Chen et al., 2007; Du et al., 2008) and more easily expresses the novel or alien traits (Figs 1, 2). In particular, chemical components from I. indigotica are also possibly produced in the hybrids (Fig. 2), as ethanol can extract cholesterol, indigotin, indirubin, p-hydroxybenzadehyde, salicylic acid, lariciresinol-4, glucopyranoside and other compounds in the plant (Sun et al., 2007).

The poor performance of the three hybrids is probably related to epigenetic factors, which might result in a hybrid displaying a phenotype either substantially better or worse than both of its progenitors (Madlung et al., 2002; Osborn et al., 2003; Wang et al., 2006; Chen, 2007; Hegarty and Hiscock, 2008), or to the numerous impacts of alien introgressions on gene regulation, largely due to epistatic or dominance effects (Baack and Rieseberg, 2007). The high frequency of transgressive or novel characters in hybrids or later generations is explained by the complementary action of new combinations of normal alleles (Rieseberg et al., 2003), the placement of unexpressed (or expressed) alleles in a new background (epistasis), the fixation of recessive alleles present in the heterozygous form in the parent (dominance), reduced developmental stability, and simple heterosis (over-dominance; Rieseberg and Carney, 1998).

The GISH signals from the I. indigotica probe were mainly located at the terminal or centromeric parts of individual bivalents or chromosomes of B. rapa ‘Aijuehuang’. The similar hybridization pattern on ‘Aijuehuang’ was also observed using Orychophragmus violaceus (Liu and Li, 2007) and Capsella bursa-pastoris probes (Chen et al., 2007). Judged from its morphology, the bivalent with its two terminals strongly labelled was most likely the satellite chromosome pair of B. rapa (Cheng et al., 1994; Hasterok et al., 2005; Liu and Li, 2007). GISH signals covered the entire length of I. indigotica chromosomes in the somatic hybrids with B. napus (Du, 2008), but mainly the centromeric parts of the Brassica chromosomes. This made detection of I. indigotica chromosomes/chromosomal segments in these hybrids more reliable.

The possible processes of cytological and genome rearrangements proposed for the formation of partial hybrids in other cases (Faure et al., 2002a; Liu and Li, 2007) would be valid for explaining the present results. When haploids (plant H3) and aneuploids (H2) are obtained, chromosome elimination possibly occurs prior to diploidization after fertilization. Complete elimination of the I. indigotica chromosomes with segmental introgression and subsequent chromosome doubling led to the formation of plant H1, and partial elimination of the I. indigotica chromosomes with segmental introgression and chromosome doubling led to the formation of H2. The production of plant H3 resulted from the complete elimination of the I. indigotica chromosomes and segmental introgression, for it also showed some characters from I. indigotica. Partial elimination without diploidization leading to production of stable additions was reported in an oat × maize cross (Riera-Lizarazu et al., 1996).

Genomic rearrangements in partial hybrids prior to meiosis have been reported in Helianthus (Natali et al., 1998; Faure et al., 2002a, b) and explained as responses to genomic shock following the interspecific cross (McClintock, 1984). Genomic shock may be one reason for the genomic changes in our hybrids. Recombination from the introgressions of I. indigotica fragments into Brassica chromosomes is another reason; however, most of the introgressed fragments were out of the range of GISH resolution (Fig. 3). The fact that the frequencies of specific AFLP bands for I. indigotica are usually lower than those of bands novel for the two parents and absent for B. rapa, especially in back-cross progenies (Tables 2, 3) suggests that other mechanisms are also involved in these genomic variations; for example: rapid sequence elimination in synthetic hybrids and allopolyploids (Song et al., 1995; Shaked et al., 2001); genomic rearrangements in the hybrids; extensive alteration in DNA methylation patterns (Natali et al., 1998; Liu et al., 2004; Wang et al., 2005) or activation of mobile genetic elements (transposons and retrotransposons; Liu and Wendel, 2000; Shan et al., 2005; Wang et al., 2005). Similarly, in rice recombinant inbred lines with <0·1 % introgressed alien DNA, extensive and genome-wide de novo variations occur in up to 30 % of the loci, with the loss of parental bands being more frequent than the gain of novel bands (Wang et al., 2005).

Hybrid formation also probably depends on the level of genetic and structural relatedness between the genomes of crops and wild plants (Leflon et al., 2006) and the similarity of the centromere structure in the two parents, or expression of the relevant function factors of the alien chromosomes in a foreign genetic background (Riera-Lizarazu et al., 1996). Tribe Isatideae, to which Isatis belongs, was first recognized by Hayek (1911) as a subtribe of the Arabideae and by Schulz (1936) as a subtribe of the Lepidieae (Al-Shehbaz et al., 2006). In the new tribal classification scheme for the family Brassicaceae, the two tribes Brassiceae and Isatideae are placed in one of three larger monophyletic lineages (Bailey et al., 2006; Al-Shehbaz et al., 2006), which suggests a closer phylogenetic relationship between the species in the two tribes. The somatic hybrids of I. indigotica with B. rapa (Tu et al., 2008) and B. napus (Du, 2008) are morphologically intermediate and show vigorous growth. The somatic hybrids with the same cultivar of B. rapa as used in this study have 2n = 48 (genomes AAIIII) and are female sterile, but partially male fertile (Tu et al., 2008). Back-cross progeny plants have also also been obtained following pollinations with B. rapa. Most of the hybrids with B. napus have 2n = 52 ( genomes AACCII), the sum of the two parents, and are partially female fertile and produce back-cross progenies (2n = 45, AACCI) after pollination by B. napus. Furthermore, the intragenomic chromosome pairing among the seven chromosomes of the I genome and intergenomic pairing between some chromosomes of the I and A/C genomes in progenies with 2n = 45 are revealed by GISH analysis (Du, 2008), indicating the homoeologous relationship between the genomes of these two species from the two tribes. The present successful synthesis of partial hybrids with genetic elements and phenotypes from I. indigotica also suggests that the species of the two tribes are related to some extent. Interestingly, the chromosome elimination occurs only in sexual but not in somatic hybrids, which may be caused by impaired centromere function (Laurie and Bennett, 1989) due to failure to recruit centromere-associated structures as kinetochore components (Mochida et al., 2004). The suppression and silencing of centromeres from one parent in hybrids most likely results from the silencing of the genes for centromere proteins and then the incongruence between their centromeric repeats and the proteins encoded by the genes of another parent. It is probable that the expression of genes from the male parent is delayed in the first zygotic divisions in the cytoplasm of the female parent, while genes from the female parent are preferentially expressed. However, in the somatic hybrid cells from parental protoplasts, where the genes from two parents are expressed, the competition and interaction of parental genes may result in dominance or co-expression. Whether the genes for centromeric proteins from I. indigotica are dominant over, or co-expressed with, those of B. napus needs to be investigated.

The occurrence of intertribal partial hybrids can provide an opportunity to rapidly alter the genomic constitution of B. rapa and introgress useful traits of I. indigotica into Brassica crops. A wide range of phenotypic variations in back-crossing progenies (2n = 20) with normal meiotic behavior can result from the introgression of alien genes, deletion of segments from incipient genomes and the production of novel segments, silencing of genes by the insertion of alien segments, or epigenetic interactions. Besides the morphological characters, variations in fatty acid content and chemical composition (Fig. 2) were also observed, but the question of whether or not medicinal or indigo components of I. indigotica are produced still needs further research. The weak plants among the progenies may be used to identify genes involved in plant development. The disomic additions with 2n = 22 are useful for introducing valuable traits and mapping genes and markers on alien chromosomes.

In conclusion, the successful synthesis of intertribal hybrids of B. rapa with I. indigotica can be viewed as a significant step towards the development of novel B. rapa types by introgressing genes of interest from I. indigotica, which will be beneficial to further broaden the Brassica gene pool. The occurrence of partial hybrids could have evolutionary consequences (Faure et al., 2002a), as mature seeds of intertribal partial hybrids that have undergone a natural process of diploidization are produced and a number of back-cross progeny plants exhibit normal growth and good seed-set. If this process takes place under natural conditions, it could lead to gene introgression between very distantly related species.

ACKNOWLEDGEMENTS

We are grateful to Professor Shyam Prakash from the Indian Agricultural Research Institute for critical reading of the manuscript, and to two anonymous reviewers for constructive suggestions to revise it. This work was supported by the National Natural Science Foundation of China (30571033 to Z.L.) and by the National Special Grant for the State Key Laboratory (to Z. L.).

LITERATURE CITED

  • Al-Shehbaz IA, Beilstein MA, Kellogg EA. Systematics and phylogeny of the Brassicaceae (Cruciferae): an overview. Plant Systematics and Evolution. 2006;259:89–120.
  • Anderson E. Introgressive hybridization. New York: John Wiley & Sons; 1949.
  • Anderson E, Stebbins GL. Hybridization as an evolutionary stimulus. Evolution. 1954;8:378–388.
  • Angelini LG, Tozzi S, o Di Nasso NN. Differences in leaf yield and indigo precursors production in woad (Isatis tinctoria L.) and Chinese woad (Isatis indigotica Fort.) genotypes. Field Crops Research. 2007;101:285–295.
  • Arnold ML. Natural hybridization as an evolutionary process. Annual Review of Ecology and Systematics. 1992;23:237–261.
  • Arnold ML. Transfer and origin of adaptations through natural hybridization: were Anderson and Stebbins right? The Plant Cell. 2004;16:562–570. [PubMed]
  • Baack EJ, Rieseberg LH. A genomic view of introgression and hybrid speciation. Current Opinion in Genetics & Development. 2007;17:513–518. [PMC free article] [PubMed]
  • Bailey CD, Koch MA, Mayer M, et al. Toward a global phylogeny of the Brassicaceae. Molecular Biology and Evolution. 2006;23:2142–2160. [PubMed]
  • Bassam B, Caetano-Anolles G, Gresshoff PM. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry. 1991;196:80–83. [PubMed]
  • Buggs RJA, Soltis PS, Mavrodiev EV, Symonds VV, Soltis DE. Does phylogenetic distance between parental genomes govern the success of polyploids? Castanea. 2008;73:74–93.
  • Chapman MA, Burke JM. Genetic divergence and hybrid speciation. Evolution. 2007;61:1773–1780. [PubMed]
  • Chen HF, Wang H, Li ZY. Production and genetic analysis of partial hybrids in intertribal crosses between Brassica species (B. rapa, B. napus) and Capsella bursa-pastoris. Plant Cell Reports. 2007;26:1791–1800. [PubMed]
  • Chen L, Lin T, Zhang HX, Su YB. Immune responses to foot-and-mouth disease DNA vaccines can be enhanced by coinjection with the Isatis indigotica extract. Intervirology. 2005;48:207–212. [PubMed]
  • Chen ZJ. Genetic and epigenetic mechanisms for gene expression and phenotypic variation in plant polyploids. Annual Review of Plant Biology. 2007;58:377–406. [PMC free article] [PubMed]
  • Cheng BF, Heneen WK, Chen BY. Meiotic studies on a Brassica campestris-alboglabra monosomic addition line and derived B. campestris primary trisomics. Genome. 1994;37:584–589. [PubMed]
  • Cheng BF, Seguin-Swartz G, Somers DJ. Cytogenetic and molecular characterization of intergeneric hybrids between Brassica napus and Orychophragmus violaceus. Genome. 2002;45:110–115. [PubMed]
  • Cronn R, Wendel JF. Cryptic trysts, genomic mergers, and plant speciation. New Phytologist. 2004;161:133–142.
  • Dellaporta SL, Wood J, Hicks JB. A plant DNA mini preparation: version II. Plant Molecular Biology Reporter. 1983;1:19–21.
  • Doyle JJ, Doyle JL, Rauscher JT, Brown AHD. Diploid and polyploidy reticulate evolution throughout the history of the perennial soybeans (Glycine subg. Glycine) New Phytologist. 2004;161:121–132.
  • Dowling TE, Secor CL. The role of hybridization and introgression in the diversification of animals. Annual Review of Ecology and Systematics. 1997;28:593–619.
  • Du XZ. China: Huazhong Agricultural University; 2008. Genetic analysis of intertribal hybrids of Brassica napus with Lesquerella fendleri and Isatis indigotica. PhD Thesis.
  • Du XZ, Ge XH, Zhao ZG, Li ZY. Chromosome elimination and fragment introgression and recombination producing intertribal partial hybrids from Brassica napus × Lesquerella fendleri crosses. Plant Cell Reports. 2008;27:261–271. [PubMed]
  • Faure N, Serieys H, Bervillé A, Cazaux E, Kaan F. Occurrence of partial hybrids in wide crosses between sunflower (Helianthus annuus) and perennial species H. mollis and H. orgyalis. Theoretical and Applied Genetics. 2002;a 104:652–660. [PubMed]
  • Faure N, Serieys H, Cazaux E, Kaan F, Bervillé A. Partial hybridization in wide crosses between cultivated sunflower and perennial Helianthus species H. mollis and H. orgyalis. Annals of Botany. 2002;b 89:31–39. [PubMed]
  • Grant V. Plant Speciation. New York: Columbia University Press; 1981.
  • Hasterok R, Wolny E, Kulak S, Zdziechiewicz A, Maluszynska J, Heneen WK. Molecular cytogenetic analysis of Brassica rapa – Brassica oleracea var. alboglabra monosomic addition lines. Theoretical and Applied Genetics. 2005;111:196–205. [PubMed]
  • Hayek A. Entwurf eines Cruciferensystems auf phylogenetischer Grundlage. Beih Botanischen Centralblatt. 1911;27:127–335.
  • Hegarty MJ, Hiscock SJ. Genomic clues to the evolutionary success of polyploid plants. Current Biology. 2008;18:R435–R444. [PubMed]
  • Hua YW, Liu M, Li ZY. Parental genome separation and elimination of cells and chromosomes revealed by AFLP and GISH analyses in a Brassica carinata × Orychophragmus violaceus cross. Annals of Botany. 2006;97:993–998. [PMC free article] [PubMed]
  • Lashermes P, Andrzejewski S, Bertrand B, et al. Molecular analysis of introgressive breeding in coffee (Coffea arabica L.) Theoretical and Applied Genetics. 2000;100:139–146.
  • Laurie DA, Bennett MD. The timing of chromosome elimination in hexaploid wheat × maize crosses. Genome. 1989;32:953–961.
  • Leflon M, Eber F, Letanneur JC, et al. Pairing and recombination at meiosis of Brassica rapa (AA) × Brassica napus (AACC) hybrids. Theoretical and Applied Genetics. 2006;113:1467–1480. [PubMed]
  • Li Z, Liu HL, Luo P. Production and cytogenetics of intergeneric hybrids between Brassica napus and Orychophragmus violaceus. Theoretical and Applied Genetics. 1995;91:131–136. [PubMed]
  • Liu B, Wendel JF. Retrotransposon activation followed by rapid repression in introgressed rice plants. Genome. 2000;43:874–880. [PubMed]
  • Liu B, Piao HM, Zhao FS, Zhao JH, Zhao R, Liu ZL. Production and molecular characterization of rice lines with introgressed traits from a wild species Zizania latifolia (Griseb.) Journal of Genetics and Breeding. 1999;53:279–284.
  • Liu M, Li ZY. Genome doubling and chromosome elimination with fragment recombination leading to the formation of Brassica rapa-type plants with genomic alterations in crosses with Orychophragmus violaceus. Genome. 2007;50:985–993. [PubMed]
  • Liu ZL, Wang YM, Shen Y, Guo WL, Hao S, Liu B. Extensive alterations in DNA methylation and transcription in rice caused by introgression from Zizania latifolia. Plant Molecular Biology. 2004;54:571–582. [PubMed]
  • Lotsy JP. Evolution by means of hybridization. The Hague: Martinus Nijhoff; 1916.
  • Luo P, Fu HL, Lan ZQ, Zhou SD, Zhou HF, Luo Q. Phytogenetic studies on intergeneric hybridization between Brassica napus and Matthiola incana. Acta Botanica Sinica. 2003;45:432–436.
  • Lysak MA, Cheung K, Kitschke M, Bures P. Ancestral chromosomal blocks are triplicated in Brassiceae species with varying chromosome number and genome size. Plant Physiology. 2007;145:402–410. [PubMed]
  • Madlung A, Comai L. The effect of stress on genome regulation and structure. Annals of Botany. 2004;94:481–495. [PubMed]
  • Madlung A, Masuelli RW, Watson B, Reynolds SH, Davison J, Comai L. Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids. Plant Physiology. 2002;129:733–746. [PubMed]
  • McClintock B. The significance of responses of the genome to challenge. Science. 1984;226:792–801. [PubMed]
  • Mochida K, Tsujimoto H, Sasakuma T. Confocal analysisi of chromosome behavior in wheat × maize zygotes. Genome. 2004;47:199–205. [PubMed]
  • Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum. 1962;15:473–479.
  • Natali L, Giordani T, Polizzi E, Pugliesi C, Fambrini M, Cavallini A. Genomic alteration in the interspecific hybrid Helianthus annuus × Helianthus tuberosus. Theoretical and Applied Genetics. 1998;97:1240–1247.
  • Osborn TC, Pires JC, Birchler JA, et al. Understanding mechanisms of novel gene expression in polyploids. Trends in Genetics. 2003;19:141–147. [PubMed]
  • Otto SP, Whitton J. Polyploid incidence and evolution. Annual Review of Genetics. 2000;34:401–437. [PubMed]
  • Prakash S, Bhat SR, Quiros CF, Kirti PB, Chopra VL. Brassica and its close allies: cytogenetics and evolution. Plant Breeding Reviews. 2009;31:21–187.
  • Riera-Lizarazu O, Rines HW, Phillips RL. Cytological and molecular characterization of oat × maize partial hybrids. Theoretical and Applied Genetics. 1996;93:123–135. [PubMed]
  • Rieseberg LH, Carney S. Plant hybridization. New Phytologist. 1998;140:599–624.
  • Rieseberg LH, Wills JH. Plant speciation. Science. 2007;317:910–913. [PMC free article] [PubMed]
  • Rieseberg LH, Van Fossen C, Desrochers AM. Hybrid speciation accompanied by genomic reorganization in wild sunflowers. Nature. 1995;375:313–316.
  • Rieseberg LH, Raymond O, Rosenthal DM, et al. Major ecological transitions in wild sunflowers facilitated by hybridization. Science. 2003;301:211–1216. [PubMed]
  • Schulz OE. Cruciferae. In: Engler A, Harms H, editors. Die natürlichen Pflanzenfamilien. Leipzig: Verlag Von Wilhelm Engelmann; 1936. pp. 227–658.
  • Seehausen O. Hybridization and adaptive radiation. Trends in Ecology and Evolution. 2004;19:198–207. [PubMed]
  • Shaked H, Kashkush K, Ozkan H, Feldman M, Levy AA. Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. The Plant Cell. 2001;13:1749–1759. [PubMed]
  • Shan XH, Liu ZL, Dong ZY, et al. Mobilization of the active mite transposons mPing and Pong in rice by introgression from wild rice (Zizania latifolia Griseb.) Molecular Biology and Evolution. 2005;22:976–990. [PubMed]
  • Song K, Lu P, Tang K, Osborn TC. Rapid genome change in synthetic polyploids of Brassica and its implications for polyploidy evolution. Proceedings of National Academy of Sciences of the USA. 1995;92:7719–7723. [PubMed]
  • Stebbins GL. Variations and evolution in plants. New York: Columbia University Press; 1950.
  • Sun DD, Li X, Chen JW, He LW. Study on chemical constituents extracted with ethanol from radix isatidis. China Pharmacy. 2007;18:2325–2326.
  • Tu YQ, Sun J, Liu Y, et al. Production and characterization of intertribal somatic hybrids of Raphanus sativus and Brassica rapa with dye and medicinal plant Isatis indigotica. Plant Cell Reports. 2008;27:873–883. [PubMed]
  • Vos P, Hogers R, Bleeker M, et al. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research. 1995;23:4407–4414. [PMC free article] [PubMed]
  • Wang J, Tian L, Lee HS, Chen ZJ. Nonadditive regulation of FRI and FLC loci mediates flowering-time variation in Arabidopsis allotetraploids. Genetics. 2006;173:965–974. [PubMed]
  • Wang QY, Wan XB. A TMV-resistant material – Banlangen (Isatis indigotica Fort.) Acta Agriculturae Boreali-Sinica. 1988;3:92–95.
  • Wang YM, Dong ZY, Zhang ZJ, et al. Extensive de novo genomic variation in rice induced by introgression from wild rice (Zizania latifolia Griseb.) Genetics. 2005;170:1945–1956. [PubMed]
  • Wendel JF. Genome evolution in polyploids. Plant Molecular Biology. 2000;42:225–249. [PubMed]
  • Zhao HJ, Huang YJ, Wang YY. A study on intergeneric hybridization between Brassica napus L. and Isatis indigotica Fort. Scientia Agricultura Sinica. 1994;27:89–91.
  • Zhong XB, Hans de Jong J, Zabel P. Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescence in situ hybridization (FISH) Chromosome Research. 1996;4:24–28. [PubMed]

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