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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Cancer Cell. Author manuscript; available in PMC 2010 July 7.
Published in final edited form as:
PMCID: PMC2705757
NIHMSID: NIHMS122956

Epidermal Notch1 loss promotes skin tumorigenesis by impacting the stromal microenvironment

Summary

Notch1 is a proto-oncogene in several organs. In the skin, however, Notch1 deletion leads to tumor formation, suggesting that Notch1 is a “tumor suppressor” within this context. Here we demonstrate that, unlike classical tumor suppressors, Notch1 loss in epidermal keratinocytes promotes tumorigenesis non-cell autonomously by impairing skin-barrier integrity and creating a wound-like microenvironment in the skin. Using mice with a chimeric pattern of Notch1 deletion, we determined that Notch1-expressing keratinocytes in this microenvironment readily formed papillomas, showing that Notch1 was insufficient to suppress this tumor-promoting effect. Accordingly, loss of other Notch paralogs that impaired the skin barrier also predisposed Notch1-expressing skin to tumorigenesis, demonstrating that the tumor-promoting effect of Notch1 loss involves a crosstalk between barrier-defective epidermis and its stroma.

Significance

In contrast to the current dogma, we demonstrate unequivocally that the non-cell autonomous consequences of defective barrier formation are responsible for the tumor-promoting effects of Notch1 loss in mouse skin. Thus, individuals with sub-acute skin-barrier defects may also be prone to carcinogenesis upon exposure to initiating carcinogens like UV rays. As Notch1 deletion in skin tumors enhanced their progression to invasive arcinomas, patients with benign hyperplasic skin lesions receiving γ-secretase inhibitor therapy may, therefore, be at additional risk. More broadly, given that chronic injury causatively effects the development of several human carcinomas, Notch1-deficient mice with mild skin-barrier defects can serve as an experimental model in which to study the tumor-promoting elements of chronic injury/wound and develop relevant therapies.

Keywords: Notch, tumor suppressor, tumor promoter, stroma, wound healing, skin-barrier defect

Introduction

Notch proteins are transmembrane receptors activated upon binding of transmembrane ligands and are implicated in many developmental and cellular processes, including carcinogenesis. Notch activation involves two sequential proteolytic cleavages releasing the Notch Intracellular Domain (NICD), a transcription regulator, into the nucleus (Lubman et al., 2004). Based on its role in most cancers involving Notch signaling (e.g. T-acute lymphoblastic leukemia), Notch1 is a proto-oncogene, driving carcinogenesis cell autonomously when hyperactivated or hyperstabilized (Radtke et al., 2006; Weng et al., 2004). In contrast, an opposite role for Notch1 is observed in skin keratinocytes, where it acts as a “tumor suppressor” (Koch and Radtke, 2007; Nicolas et al., 2003). The mechanism enabling Notch1 to deliver this unique function in skin remains controversial.

The vertebrate skin is a barrier-forming organ in which keratinocytes form a highly organized, stratified epithelium protecting the internal milieu from the outside environment. To achieve this, proliferating keratinocyte progenitors residing within the innermost (basal) layer of the epidermis constantly divide and replenish the upper barrier-forming layers (Clayton et al., 2007). Cells exiting the basal layer gradually commit to terminal differentiation in the spinous layer, and, under normal conditions, complete their differentiation program, giving rise to granular and cornified layers (Fuchs and Raghavan, 2002; Jamora and Fuchs, 2002). Three Notch paralogs (Notch1, 2 & 3) are expressed in the epidermis and their activation is evident in suprabasal keratinocytes. Importantly, reduction in Notch signaling within keratinocytes impairs their ability to execute the terminal differentiation program, resulting in formation of a defective skin-barrier and death if the areas involved are sufficiently large (Blanpain et al., 2006; Demehri et al., 2008).

One of the major adverse consequences of Notch loss in epidermis is the development of skin tumors (Nicolas et al., 2003; Proweller et al., 2006), evoking the use of the term tumor suppressor to specifically describe the role of Notch1 in the epidermis (Nicolas et al., 2003). However, the exact mechanism underlying Notch1 tumor suppressor function, and why specific consequences of Notch1 loss cannot be compensated for by other Notch paralogs present in the skin (i.e. Notch2 & 3), are not fully understood. It has been proposed that the tumor suppressor activity of Notch1 reflects its unique ability to antagonize keratinocyte proliferation through one or more cell autonomous signaling mechanisms. Most of these findings were established in pre-cancerous hyperplastic epidermis of Notch-deficient animals (Nicolas et al., 2003; Proweller et al., 2006) and did not examine the early changes following Notch loss. Other conclusions were based on in vitro studies with isolated keratinocytes (Devgan et al., 2005; Nguyen et al., 2006; Nicolas et al., 2003), failing to take into account the complexity of the skin microenvironment including the contribution of other skin components to carcinogenesis. Notably, in vivo studies have revealed that loss of Notch signaling during embryogenesis induces epidermal hypoplasia and low proliferative capacity in the keratinocytes, proposing that reactive, i.e. secondary, hyperplasia accounts for the late epidermal hyperproliferation detected in adult Notch-deficient skin (Blanpain et al., 2006; Demehri et al., 2008). This also implies that the molecular changes outlined above may reflect secondary events following epidermal hyperplasia in Notch-deficient mice.

In this study, we investigate the mechanism underlying tumor development in Notch1-deficient skin in vivo using the multistage skin chemical carcinogenesis model. In this well-established carcinogenesis model, treating the skin with an initiating carcinogen, 7,12-dimethylbenz[a]anthracene (DMBA), results in an activating mutation in the H-ras gene and creation of “initiated cells.” Thereafter, the continual exposure of the skin to a tumor promoting agent, tetradecanoylphorbol acetate (TPA), leads to expansion of the initiated cells and eventually to tumor development. In addition, we took advantage of two Cre-expressing transgenes. K14CreERT (Vasioukhin et al., 1999) allows us to generate mice in which we could control the timing of epidermal Notch1 deletion relative to DMBA treatment. Msx2-Cre is ectopically expressed at E9.5 in clusters of ectodermal cells prior to the onset of skin morphogenesis but its expression is never again detected in the epidermis after E13. This chimeric pattern of Cre expression allows us to generate mice with a chimeric pattern of Notch1 deletion in skin keratinocytes with three types of epidermal territories: (1) clones of epidermal cells on dorsal and ventral midline with complete deletion of Notch1-floxed alleles showing alopecia; (2) Territories with functional proteins expressed from un-deleted floxed alleles exhibiting normal epidermal and hair growth; and (3) Border regions between the two in which cells of both genotypes mingle (Demehri et al., 2008; Pan et al., 2004) (Figure S1). This chimeric animal model permits a direct comparison of cells with identical genetic backgrounds, other than the deleted allele(s), in the same microenvironment.

Results

Animals with chimeric loss of Notch1 in the epidermis develop skin tumors

As reported in animals with 4-hydroxytamoxifen (4-OHT)-induced deletion of Notch1 in the epidermis (Nicolas et al., 2003), most of 20-month-old Msx2-Cre; Notch1flox/flox (Msx2-N1CKO) mice spontaneously developed 1 to 4 skin tumors each (Figure 1A). These lesions were predominantly composed of benign papillomas; however, four out of 41 spontaneous tumors characterized progressed to basal cell carcinoma (BCC; Figure 1B). To study the mechanism linking Notch1 loss to skin carcinogenesis in an in vivo setting that permitted analysis of mutant and wild-type cells in the same environment, we examined the response of Msx2-N1CKO animals to the multistage chemical skin carcinogenesis model (Figure 1C). Treating 6- to 10-week-old Msx2-N1CKO and wild-type (Notch1flox/flox) littermates with a single initiating dose of DMBA, followed by a twice-weekly dose of TPA for 14 weeks (Nicolas et al., 2003), resulted in development of more than 20 papillomas/mouse in all DMBA/TPA-treated Msx2-N1CKO animals, whereas none of the control animals developed any papillomas after 25 weeks of follow-up (Figures 1D–E, Tables S1 and S2). These results reproduce the tumor phenotype reported (Nicolas et al., 2003) and establish that Notch1 loss, even in a fraction of epidermal cells, is sufficient to sensitize the animals to chemical carcinogenesis.

Figure 1
Msx2-N1CKO mice develop skin tumors spontaneously and in response to chemical carcinogens. A) Spontaneous skin tumorigenesis of Msx2-N1CKO mice and their wild-type littermates (n=20 for each group; p <0.0001, log rank test). B) A representative ...

Notch1 loss primarily acts as a tumor promoter in the epidermis

We next tested the impact of Notch1 loss on distinct stages of skin tumor development (Zoumpourlis et al., 2003). The appearance of spontaneous tumors on Msx2-N1CKO skin could reflect a role for Notch1 in initiation, in which case tumors would be expected to develop earlier in Msx2-N1CKO mice exposed to TPA alone. However, 6- to 10-week-old Msx2-N1CKO animals treated only with a high dose of TPA twice weekly for 25 weeks developed hyperplasia but did not develop any tumors (n=10, Figure 2A), indicating that deletion of Notch1 did not act as a tumor initiator, nor did its loss lead to the activation of one. Accordingly, we were unable to detect an elevated Gli2 expression or elevated Wnt/β-catenin signaling (seen at 6 weeks of age or older) (Nicolas et al., 2003; Proweller et al., 2006) in 2-week-old Notch-deficient skin (Figure S2). However, given that the direct Notch1 targets in skin keratinocytes, Hes1 and Hey1, are repressors of HDM2 (Huang et al., 2004), Notch1 loss might have led to elevated MDM2 and destabilization of p53 thereby facilitating H-ras-mediated transformation of DMBA-treated keratinocytes (Zhao et al., 2006). In addition, Notch1 loss could contribute to tumor initiation by lowering p21WAF1/Cip1 expression in keratinocytes (Nicolas et al., 2003; Rangarajan et al., 2001). Therefore, we examined the possibility that Notch1 loss contributed to the fixation rate of DMBA-induced DNA mutation in basal keratinocytes. We treated 6- to 10-week-old K14CreERT; Notch1flox/flox (K14ERT-N1CKO) animals with 4-OHT to remove Notch1 either before (OHT→ DMBA→ TPA, or ODT) or after (DMBA→ OHT→ TPA, or DOT) DMBA exposure (Figure 2B). As judged by time to tumor onset, both cohorts displayed a similarly elevated susceptibility to carcinogenesis over the 4-OHT-treated controls (K14CreERT; Notch1flox/+), indicating that Notch1 loss did not contribute to a p21WAF1/Cip1- or p53-controlled checkpoint (Figure 2C and Table S1). Although the ODT cohort developed more tumors in the final two weeks of the follow-up period, both cohorts had comparable tumor counts during the first 23 weeks that were significantly higher than the wild-type tumor count (Figure 2D and Table S1). Although our animals were kept in an outbred background with variable strain susceptibility, nested ANOVA analysis showed that the differences reported in this study were solely conferred by the presence or absence of Notch1 (Tables S3). Collectively, these data suggest that the main consequence of Notch1 loss is in tumor promotion. Indeed, 100% of Msx2-N1CKO mice treated only once with DMBA (n=10) developed tumors, whereas none of the wild-type controls (Notch1flox/flox; n=10) did (p <0.0001, Figures 2E–F, Tables S1 and S4).

Figure 2
Epidermal deletion of Notch1 leads primarily to skin tumor promotion. A) An example of Msx2-N1CKO and wild-type skins with high doses of TPA (18μg per mouse, arrow) twice weekly for 25 weeks (scale bar: 100μm). B) Schematic diagram detailing ...

As expected (Nicolas et al., 2003), subset of papillomas from the ODT and DOT groups progressed to invasive squamous cell carcinoma (SCC) (Figure S3). Therefore, we examined the impact of Notch1 loss on malignant progression. Intraperitoneal injection of 4-OHT into K14ERT-N1CKO and control mice after completion of the DMBA/TPA protocol (DTO) did not alter tumor numbers (Figures 3A–B). Interestingly, malignant conversion of papillomas to metastatic SCC was seen only in DTO animals (Figures 3C–D). On average, 50% of Notch1-deficient tumors progressed to SCC, which metastasized and necessitated euthanasia of the moribund mice (Figure 3E). Together, these in vivo cancer studies indicate that the primary role of Notch1 in suppressing skin tumors is in blocking tumor promotion and progression; the latter could be associated with reduction in p53 (Huang et al., 2004) or with elevated activity of Wnt and HH signaling in hyperplastic, Notch1-deficient cells (Nicolas et al., 2003).

Figure 3
Notch1 loss in the epidermis causes malignant progression of skin tumors. A) Schematic diagram outlining the method used to address the role of Notch1 in tumor progression. Notch1 deletion was induced after papilloma formation in K14CreERT background. ...

Notch1-deficient epidermis creates a microenvironment promoting carcinogenesis

The tumor-promoting effect of Notch1 deletion can be mediated by cell autonomous changes in the initiated cells that acquired H-ras mutations, by non-cell autonomous signals emanating from the surrounding environment that is responding to signals produced by Notch1-deficient keratinocytes (Lee et al., 2007), or by combination of both. To determine which of these possibilities best describes the contribution of Notch1 loss to skin tumorigenesis, we reanalyzed Msx2-N1CKO mice exposed to DMBA/TPA (Figure 1) and performed an intracomparison of adjacent wild-type and Notch1-deficient territories within each animal after DMBA/TPA treatment (Figure 4A). If Notch1 acted like a classical tumor suppressor, initiated cells expressing Notch1 would not be able to form tumors. In contrast to this prediction, DMBA/TPA-treated Msx2-N1CKO; Rosa26R mice had a comparable number of Cre-negative (white) papillomas adjacent to the Cre-positive (blue) territory (Figure 4B). This is consistent with Notch1-expressing tumors developing in Msx2-N1CKO; Rosa26R animals. To confirm that tumors can arise from initiated cells that did not experience Notch1 deletion, we performed direct amplification of the Notch1 locus in tumor DNA randomly collected from DMBA/TPA-treated Msx2-N1CKO animals to estimate how many cells underwent Notch1 deletion (Figures 4C and S4). Notch1 deletion was undetectable in 33 out of 91 benign exophytic papillomas analyzed by PCR with conditions sufficient to detect deletion in 1% of genome equivalents. Since a significant subset of papillomas contained more than 99% of genome equivalents contributed by wild-type cells, we concluded that these tumors arose from initiated cells that did not experience Notch1 deletion. Because none of the wild-type littermates used in this study (n=10) developed any tumors, this finding demonstrates that loss of Notch1 provides a promoting, non-cell autonomous signal that can support papilloma formation from nearby initiated cells independent of their Notch1 status. Given the absence of SCC among DMBA/TPA-treated Msx2-N1CKO animals and the global nature of Cre activation in K14ERT-N1CKO mice (Vasioukhin et al., 1999), we could not examine if tumor progression to SCC was a strict cell autonomous consequence of Notch1 loss.

Figure 4
Notch1-deletion in epidermal keratinocytes acts as a non-cell autonomous tumor promoter. A) The chimeric pattern of Notch1 deletion in Msx2-N1CKO animals enables us to determine if the tumor-promoting effect of Notch1 loss in the skin is the consequence ...

Epidermal differentiation/barrier formation defects can explain tumor promotion upon Notch1 loss

Loss of Notch signaling in the skin causes impaired epidermal differentiation, which in turn results in defective skin-barrier formation (Figure S5) (Blanpain et al., 2006; Demehri et al., 2008). After birth, the reactive epidermal hyperplasia masks the physical consequences of skin-barrier impairments (i.e. dye penetration or transepidermal water loss) to ensure survival in terrestrial life (Kuramoto et al., 2002). Nonetheless, thymic stromal lymphopoietin (TSLP) and antimicrobial peptide overexpression can serve as reliable biomarkers for postnatal barrier impairments (Aberg et al., 2008; Demehri et al., 2008; Kuramoto et al., 2002). Based on this criteria, a mild barrier defect is also detectable in Msx2-N1CKO skin at P9 (Figure S6A; (Demehri et al., 2008)). The persistence of this barrier defect was confirmed by documenting the overexpression of antimicrobial peptides of mRNA in the epidermis (Figure S6B) and upregulation of serum TSLP levels in adult Msx2-N1CKO mice (Figure S7).

Notch pathway-deficient skin present initially with an epidermal hypoplasia that resembles a chronic wound, thus recruiting an array of cellular responders to repair the site of the breach and resulting in the development of a reactive epidermal hyperplasia overtime (Blanpain et al., 2006; Segre, 2006). Accordingly, Msx2-N1CKO epidermis was mildly hypoplastic at birth, but exhibited a significant stromal hyperplasia as early as P9 (Figure 5A). Following the proliferative changes in the dermis, significant epidermal hyperplasia developed in adult mutants (Figures 5A and S8; (Lee et al., 2007)). Importantly, epidermal hyperplasia extended beyond Notch1-deleted territories and into adjacent Notch1-expressing epidermal regions (Figure 5B), indicative of a non-cell autonomous proliferative mechanism.

Figure 5
Notch1-deficient epidermis creates a wound-like microenvironment in the skin. A) H&E staining of Msx2-N1CKO and wild-type (Wt) skin at birth (P0), P9 and 1 year of age demonstrates the progression from relatively hypoplastic epidermis in newborn ...

The major stromal responses to a breach in the skin barrier include infiltration of immune cells, activation of fibroblasts and angiogenesis, all of which provide proliferative signals to keratinocytes as part of an integrated wound healing/barrier repair response (Segre, 2006; Werner et al., 2007). To determine the contribution of immune cells to reactive epidermal hyperplasia and tumor promotion in Msx2-N1CKO skin (Proweller et al., 2006), we examined the number and composition of dermal leukocytes in Msx2-N1CKO skin. An increase in CD45+ leukocytes was evident in Msx2-N1CKO skin by P9 (Figure 5C), which was due predominantly to accumulation of CD4+ T cells in the dermis of Msx2-N1CKO mice (Figures 5D and S9). Prominent increase in number of CD4+ T cells in dermis and peripheral blood of adult Msx2-N1CKO mice together with serum IgE elevation (Figure S10) is reminiscent of a mild atopic dermatitis (AD)-like allergic inflammation, which represents a typical immune response to skin-barrier defects (Segre, 2006). In addition, dermal mast cells, another component of AD-like inflammation (Navi et al., 2007), were significantly increased in Msx2-N1CKO skin (Figure S11). Mast cell activation can enhance skin carcinogenesis (de Visser et al., 2005). To examine if this was critical for tumor promotion, we impaired mast cells and neutrophils function by generating Msx2-N1CKO mice deficient for cathepsin C (dipeptidyl peptidase I; DPPI (Pham, 2006). DMBA/TPA treatment of Msx2-Cre; Notch1flox/flox; DPPI−/− (Msx2-N1CKO;DPPI−/−), Msx2-N1CKO, DPPI+/− and wild-type littermates demonstrated that mast cells did not play a dominant role in tumor promotion since the inhibition of mast cell/neutrophil function did not completely ameliorate the tumor promoting effect of the stroma in Notch1-deficient background (Figures 5E–F). When nested ANOVA was applied to account for the contribution of gender-based differences, a trend toward delayed tumor onset, and a significant reduction in tumor counts at 20 weeks after DMBA treatment, were detected in Msx2-N1CKO;DPPI−/− relative to Msx2-N1CKO mice (Table S5). Thus, mast cells contribute to tumor promotion, but loss of this contribution can be largely overcome by other tumor-promoting factors.

Msx2-N1CKO epidermis overproduces TGF-β1 and TGF-β2 (Lee et al., 2007), the major diffusible keratinocyte factors that recruit Gr-1+CD11b+ myeloid suppressor cells (Yang et al., 2008) and activate dermal fibroblasts (Werner et al., 2007). Gr-1+CD11b+ myeloid suppressor cells, which promote carcinogenesis by suppressing immunosurveillance apparatus including cytotoxic CD8+ T cells (Bronte et al., 2000), were increased in spleen of adult Msx2-N1CKO (Figure S12). Activated fibroblasts, known to play an important role in tumor promotion (Orimo and Weinberg, 2006), could also secrete matrix-modifying proteins and mitogens leading to epidermal hyperproliferation, establishing a “vicious cycle” (Lee et al., 2007). In agreement with a vicious cycle involving TGF-β signaling (Werner et al., 2007), Msx2-N1CKO dermal fibroblasts were increased relative to wild type at P9 and 1 year of age, acquiring a myofibroblast phenotype as indicated by the expression of α-smooth muscle actin (α-SMA) in dermis of adult Notch1-deficient mice (Figures 5G–H and S13). Accordingly, the expression levels of two fibroblast-derived epidermal mitogens, keratinocyte growth factor (KGF or FGF-7) and stromal cell-derived factor 1 (SDF-1 or CXCL-12) (Szabowski et al., 2000; Werner et al., 2007), were modestly but significantly up-regulated in Notch1-deficient skin at P9 (1.6 fold, p <0.001; Figure 5I). SDF-1 mRNA remained elevated in 6- to 8-month-old Msx2-N1CKO skin relative to wild-type littermates, confirming the persistent overexpression of this factor overtime (Figure S14). Of note, the dermal vasculature also showed increased branching and dilation in P9 and adult Msx2-N1CKO skin, underscoring the gestalt of non-cell autonomous events contributing to the tumor phenotype (Figures 5G&J). Taken together, the consequences of persistent skin-barrier defects in Msx2-N1CKO mice create a wound-like, proliferative microenvironment capable of driving epidermal hyperplasia and carcinogenesis overtime (Alberts et al., 2002; Eming et al., 2007; Parkinson, 1985).

All Notch paralogs involved in barrier formation also participate in suppressing skin carcinogenesis

Loss of either Notch2 (Msx2-Cre; Notch2flox/flox or Msx2-N2CKO) or Notch3 (Notch3−/− or N3KO) in the skin had no phenotypic consequences, and accordingly, no spontaneous epidermal tumors appeared over the entire life span of these animals (n>10 for each genotype; Figure S15A). Furthermore, the response of Notch2- or Notch3-deficient mice to DMBA/TPA carcinogens was indistinguishable from the wild-type littermates, reflecting their respective strain’s baseline susceptibility (Figures S15B-C). This result could reflect a unique contribution of Notch1 to tumor suppression, perhaps by maintaining p21WAF1/Cip1 expression (Devgan et al., 2005; Nguyen et al., 2006; Nicolas et al., 2003; Okuyama et al., 2004). Alternatively, if tumor promotion is mainly a consequence of an impaired epidermal differentiation/barrier formation (the “defective barrier” hypothesis), the lack of tumor susceptibility upon the deletion of Notch2 or Notch3 could be because their loss, unlike Notch1, did not compromise the skin barrier.

To differentiate between these possibilities, we first accessed the contribution of Notch2 and Notch3 to the Notch1 “tumor suppressor” function by analyzing an allelic series in which Notch2 and Notch3 alleles were progressively removed on the Msx2-N1CKO background. Removal of Notch2 and Notch3 in Notch1-deficient skin exacerbates the barrier defects (Demehri et al., 2008). In agreement with the defective barrier hypothesis, this stepwise removal of Notch2 and Notch3 alleles in Msx2-N1CKO animals resulted in progressive enhancement of epidermal hyperplasia at P9 (Figures 6A–B). We also noticed a tight correlation between time to spontaneous tumor onset and global Notch dose in keratinocytes (Figure 6C). This demonstrates the existence of an additive function for Notch paralogs in suppressing skin tumors as long as the overall Notch dosage is reduced below a threshold; however, it does not determine whether Notch1 makes a unique contribution to tumor suppression in addition to its shared effect on proper skin-barrier formation.

Figure 6
Stepwise removal of Notch paralogs in Msx2-N1CKO animals leads to progressively worse epidermal hyperplasia that accelerates the onset of spontaneous skin carcinogenesis. A) Skin histology of compound Notch-deficient mice at P9 demonstrates progressive ...

To examine if Notch1 has any unique tumor suppressing activity sufficient to prevent tumor formation in barrier-impaired skin, we generated animals lacking Notch2 and Notch3 while retaining the expression of Notch1 (Msx2-Cre; Notch2flox/flox; Notch3−/− or Msx2-N2N3CKO). P9 Msx2-N2N3CKO epidermis overexpressed TSLP and antimicrobial peptides, demonstrating that keratinocytes lacking Notch2 and Notch3 formed a defective skin-barrier (Figures 7A and S6). TSLP overexpression persisted in adult Msx2-N2N3CKO mice and was accompanied by serum IgE elevation that signified the development of a subacute AD-like inflammation in response to persistent barrier defects in these animals, similar to Msx2-N1CKO mice (Figures 7B–C, S6 and S10B). Importantly, Msx2-N2N3CKO epidermis retained normal expression of p21wAF1/Cip1, confirming the context-specific role of Notch1 in regulating p21wAF1/Cip1 expression (Figure 7A; (Nicolas et al., 2003)). Nonetheless, deletion of Notch2 in the skin of Notch3 null animals resulted in skin hyperplasia (Figure 7D) and spontaneous tumors in the skin of adult Notch1-expressing Msx2-N2N3CKO animals (Figures 7E–F). As predicted from the defective barrier hypothesis, exposure of Msx2-N2N3CKO mice to DMBA/TPA resulted in a significantly higher tumor burden than that seen in control littermates (Figures 7G–H), with many tumors progressing to SCC (Figure 7I). Thus, blocking tumor progression is a p21wAF1/Cip1 independent, shared function of the three Notch receptors. Taken together, these findings indicate that the presence of Notch1 and p21wAF1/Cip1 is not sufficient to protect barrier-defective skin from chemical carcinogens and instead demonstrates that the tumor phenotype mirrors the progressive defects in barrier formation and keratinocyte differentiation.

Figure 7
Mice lacking Notch2 and Notch3 in the skin develop skin-barrier defect, epidermal hyperplasia and skin tumors. A) TSLP overexpression (a biomarker for postnatal skin-barrier defects (Demehri et al., 2008)) is seen in both Notch1-deficient and Notch2/3 ...

Discussion

The fundamental observation that Notch1 deletion in epidermal keratinocytes causes skin carcinogenesis is a clear deviation from Notch1’s role as a proto-oncogene in several other organs (Koch and Radtke, 2007). We examined the mechanism underlying the tumor-prone behavior of Notch1-deficient skin in mice with a global or chimeric deletion pattern in their epidermis. We established that Notch1 resembled most tumor suppressors in that its loss was not involved in the initiating event of multistage skin carcinogenesis (Zoumpourlis et al., 2003) by deleting Notch1 either before or after DMBA treatment in the K14CreERT system. However, Notch1 loss could effectively substitute for TPA in the chemical carcinogenesis paradigm, establishing unequivocally that its loss acts as a tumor-promoting event. Delaying Notch1 deletion in K14CreERT mice until after the tumor-promotion stage of carcinogenesis demonstrated that late deletion of Notch1 contributed to malignant progression of benign papillomas, a phenotype that is observed upon loss of p53 but not loss of p21WAF1/Cip1 (Weinberg et al., 1999), a specific Notch1 target in the skin (Rangarajan et al., 2001). Taken together, we have conclusively determined that the main effect of Notch1 loss is to provide the initiated cells with a proliferative signal to form tumors and proceed to invasive carcinoma.

The proliferative signal that lies downstream of Notch1 loss could be originated from within the initiated cells, substantiating Notch1’s role as a classical tumor suppressor in skin keratinocytes (Nicolas et al., 2003). Alternatively, this signal could be delivered by the skin microenvironment reacting to Notch1 loss in the epidermis (Lee et al., 2007; Orimo and Weinberg, 2006; Vauclair et al., 2007; Watt et al., 2008). The system we studied allowed us to distinguish between these two possibilities; the chimeric pattern of Notch1 deletion by Msx2-Cre created neighboring territories of Notch1-expressing and Notch1-deficient keratinocytes coexisting in the same microenvironment. Examining a large number of tumors isolated from DMBA/TPA-treated Msx2-N1CKO mice clearly demonstrated that tumors comprised mostly (>99%) of Notch1-expressing cells were as likely to form as tumors comprised predominantly of Notch1-deleted cells in the same environment. Thus, Notch1 loss in the epidermis generates a non-cell autonomous signal, promoting tumorigenesis from any initiated cell exposed to the microenvironment conditioned by Notch1-deficient keratinocytes. This finding emphasizes the importance of the environment as an active contributor to tumor development (Bissell and Radisky, 2001) by showing that it can be the primary source of proliferative signals to initiated cells.

To determine the identity of the tumor-promoting microenvironment formed as a consequence of Notch1 deletion in keratinocytes, we reexamined the earliest effects of Notch1 loss on the skin. As previously shown, loss of Notch signaling leads to impaired keratinocyte proliferation/differentiation culminating in epidermal cell loss and defective skin-barrier function at birth (Blanpain et al., 2006; Demehri et al., 2008; Rangarajan et al., 2001). Therefore, we examined the hypothesis that Notch1-deficient skin encompassed a chronic wound-like microenvironment developing in response to barrier defects, which were the direct consequence of Notch1 deletion in the epidermis (Blanpain et al., 2006; Demehri et al., 2008). Indeed, the dermis of Notch1-deficient skin contained the critical components of an activated stroma responding to the breach in the skin barrier including inflammatory cell infiltrate, activated fibroblasts and expanded vasculature (Mueller and Fusenig, 2004). To further demonstrate that tumor promotion was the consequence of an activated stroma responding to a general breach in the skin barrier, we showed that mice lacking Notch2 and Notch3 in their epidermis also developed skin tumors. This is in contrast to a mechanistic model proposing that cell autonomous oncogenic changes, specific to Notch1 loss (Rangarajan et al., 2001), are the initial events mediating tumorigenesis in Notch1-deficient skin. We find that the tumors in Notch1-deficient skin are the end product of a complex interaction between a barrier-defective epidermis and its underlying stroma, which creates a tumor-promoting feed-forward loop. This Notch1-independent, barrier-dependent phenotype distinguishes Notch1 from classical tumor suppressors. Accordingly, we predict that any mouse model with mild chronic skin-barrier defects will also be prone to skin tumorigenesis.

Fibroplasia, angiogenesis and inflammation are stromal elements intimately linked to wound repair (Martin, 1997). These cellular changes are also closely associated with neoplastic transformation (Bissell and Radisky, 2001; Mueller and Fusenig, 2004). It is proposed that the microenvironment of the non-healing wound/defective skin barrier could be a risk factor for carcinogenesis (Eming et al., 2007). This association is supported by chemical carcinogenesis studies showing that tumors grow at the edges of skin wounds (Parkinson, 1985). In addition, it is suggested that chronic injury can predispose various organs to cancer (Bissell and Radisky, 2001), and there is clinical evidence linking chronic skin wounds to BCC and SCC (Nguyen and Ho, 2002). For instance, leg ulcers significantly increase the risk of SCC in patients (Baldursson et al., 1995). Nonetheless, experimental evidence establishing that stromal changes in chronic wound microenvironment can drive skin carcinogenesis is lacking (Eming et al., 2007). Therefore, Msx2-N1CKO and Msx2-N2N3CKO skin present a model demonstrating that a lengthened stromal attempt to repair a non-healing wound or a persistent skin-barrier defect predisposes the skin to carcinogenesis. We speculate that the plurality of the cellular effectors (i.e. Fibroplasia, angiogenesis and inflammation) responding to breach in skin-barrier collectively contribute to tumor promotion in this model.

We have previously identified several of these factors. Matrix metallopeptidases (MMP8 and MMP9) are elevated in Notch1-deficient skin at P9 as is osteopontin (OPN (Demehri et al., 2008)). All these stromal-derived factors are potential tumor promoters (Pazolli et al., 2009; van Deventer et al., 2008). In addition, dermal fibroblasts in Notch1-deficient skin are overproducing SDF-1 and KGF that directly stimulate keratinocytes proliferation; this is reminiscent of carcinoma-associated fibroblasts known to promote tumor development from a non-tumorigenic cell population (Bhowmick et al., 2004; Orimo and Weinberg, 2006). Furthermore, accumulation of immune cells and development of a subacute inflammation in Notch1-deficient skin, triggered by cytokines/chemokines released from barrier-defective epidermis (Demehri et al., 2008; Segre, 2006; Yoo et al., 2005), have been shown to promote skin carcinogenesis (Johansson et al., 2008). From this large pool of tumor-promoting stromal cells/factors present in Notch1-deficient skin, we examined the contribution of a single component (Mast cells), which has been previously deemed critical in skin carcinogenesis (Coussens et al., 1999; de Visser et al., 2005; Tlsty and Coussens, 2006), and asked if the components listed above act redundantly or are all required individually. Our results are consistent with the possibility that removal of any single stromal component would not significantly alter the tumor-promoting effect of the wound microenvironment. Therefore, we propose that the tumor promotion in Notch1-deficient skin results from the additive contributions of fibroplasia, angiogenesis and inflammation. The cumulative effect of these factors on skin carcinogenesis in the presence of severe barrier defects that cause a full-blown inflammatory disease (i.e. atopic dermatitis) remains a topic for future investigation. Taken together, Notch1 and Notch2/3-deficient mice demonstrate that stroma of a chronic skin wound is analogous to tumor stroma, and can be used to determine the specific contribution of each stromal component to tumor development, a worthy question that falls outside the scope of the current study.

In conclusion, the persistent barrier defects in Notch-deficient skin, which resembles chronic wounds, recruit several mesenchymal components necessary to repair the barrier. In turn, the vascularized and growth-factor-rich stroma provides initiated cells with nutrients and proliferative signals that can directly promote tumor formation. Thus, Notch1 is not a classical tumor suppressor that solely exerts its effects cell autonomously (e.g. promotes cell death or cell cycle arrest). Notch-deficient mice provide instead a suitable system in which to dissect out the molecular mediators and the cellular interactions that are responsible for oncogenic effect of chronic wound/tumor stroma. Based on such an analysis, new therapeutic targets can be identified in the tumor microenvironment that will be useful in developing molecular therapies for cancers of skin and perhaps other organs (Albini and Sporn, 2007).

Experimental Procedures

Generation of Mutant Mice

All the mice were maintained in the Washington University animal facility according to animal care regulations, and the Animals Studies Committee of Washington University approved the experimental protocols.

The mutant strains of mice analyzed in the current study were generated following the protocol described previously (Pan et al., 2004). All the animals were maintained in mixed C57BL/6 and CD1 genetic backgrounds, which were overall resistant to DMBA/TPA skin carcinogenesis. In some experiments, remnant contributions from 129sv and FVB strains might have also been present. In all cancer experiments, age-matched littermates were compared, and nested ANOVA was used to confirm that strain-based differences did not confound our analysis. In studies related to spontaneous carcinogenesis and longevity, mice were monitored regularly for onset, number and size of tumors and any sign of failure to thrive. Moribund mice are euthanized and skin, tumors, and lymph nodes are harvested.

Chemical Skin Carcinogenesis Studies

For DMBA/TPA experiments in Msx2-Cre background, mutant mice and their age-matched littermate controls were treated with standard protocols for skin chemical carcinogenesis models as previously described (Nicolas et al., 2003). Further details are presented in supplemental information.

Histology, Imunohistochemistry and Flow Cytometry (FC)

For Hematoxylin and eosin (H&E), toluidine blue and immunostaining using paraffin-embedded tissue sections, skin, tumor and lymph node samples from various mutant and wild-type animals were fixed in 4% paraformaldehyde in PBS, dehydrated with ethanol and embedded in paraffin, which were then sectioned at 5μm. X-gal staining was done on the skin prior to fixation with 4% paraformaldehyde as previously described (Pan et al., 2004). Antibodies used for immunohistochemistry are listed in the supplemental information. For FC analysis, single cell suspensions from dermis, peripheral blood and spleen were prepared as described (Demehri et al., 2008). Dermal cells were isolated using a Brinkmann Tissue Chopper (ON, Canada) and crude collagenase (Sigma) digestion for 90min at 37°c. Single cell suspensions were stained with antibodies listed in the supplemental information.

ELISA and Immunoblotting

Serum TSLP concentrations were measured using Quantikine mouse TSLP kit (R&D Systems, Minneapolis, MN). Serum IgE was measured using Mouse IgE ELISA kit (Immunology Consultants Laboratory Inc., Newberg, OR). Epidermal samples were collected in NP40 lysis buffer as previously shown (Lee et al., 2007; Nicolas et al., 2003) Protein lysates were run on SDS-PAGE gels after adjusting for protein concentration and analyzed using anti-active β-catenin antibody (ABC, Upstate Biotechnology, Lake Placid, NY) and total β-catenin (BD PharMingen).

Dye Penetration Assay

To detect defect in skin-barrier function (Hardman et al., 1998), intact E18.5 embryos were stained in X-gal (pH 4.5) for 12hr at 37°C. After X-gal staining, the embryos were washed in PBS three times and photographed with a digital camera.

PCR and qRT-PCR

Conventional PCR for Notch1 allele was performed on genomic DNA isolated from skin tumors of DMBA/TPA-treated Msx2-N1CKO mice using KlenTaq10 (DNA Polymerase Technology, St. Louis, MO) supplemented with 1.3M final concentration of betaine (amplification cycles=32). qRT-PCR was performed on mRNA isolated from skin and epidermis of Msx2-N1CKO, Msx2-N2N3CKO mice and their wild-type littermates as previously described (Lee et al., 2007). The primers used are listed in Table S7.

Statistical Analysis

The studies in this report were conducted in outbred cohorts of mice resembling human population. To minimize the effect of susceptibility differences due to genetic background on tumor phenotype observed in each study, age-matched littermates were used as controls. We used power analysis to estimate the number of mice needed in each group to reach statistical significance (Table S8). In addition, to confirm that the differential tumor parameters we measured were conferred by status of gene (e.g. Notch1) deletion and not by heritable factors (strain) or gender, we used nested ANOVA (SPSS, Chicago, IL; Tables S2–6). Further details are presented in supplemental information. “Time to tumor onset” and “survival” data were analyzed using log rank test to determine significant differences. Tumor counts and other quantitative measurements were assessed using Student’s t-test. These quantitative data are presented as mean ± standard deviation for each measured parameter.

Supplementary Material

01

Acknowledgments

We would like to thank Drs. James M. Cheverud and Catherine M. Roe for critical guidance with statistical analysis, and Dr. Anne Lind for help with determining the skin tumor types. We thank Dr. Yong-Hua Pan and other members of the Kopan laboratory for their suggestions and assistance during the course of this study. The authors wish to thank Mrs. Tao Shen and Yumei Wu for genotyping and for assistance in caring for the mice involved in this study. We thank Drs. Jeffrey Arbeit, Timothy Ley, Sheila Stewart, Jason Weber, Helen Piwnica-Worms and Gregory Longmore for commenting on the manuscript and providing many valuable suggestions. We would like to thank Dr. Gail Martin for providing the Msx2Cre mice, Dr. Tom Gridley for the Notch2flox/flox mice, Dr. Jie Shen for PS1flox/flox mice, Dr. Tasuku Honjo for RBP-jflox/flox mice, and Dr. Elaine Fuchs for the K14-CreERT mice. Finally, we wish to thank the reviewers for the careful and constructive critique of this study. RK, AT and SD are supported by grant GM55479-10 from NIH/NIGMS.

Footnotes

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