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Ann Bot. 2009 June; 103(9): 1379–1383.
Published online 2009 February 14. doi:  10.1093/aob/mcp030
PMCID: PMC2701766

Pollinator visitation patterns strongly influence among-flower variation in selfing rate

Abstract

Background and Aims

Adjacent flowers on Mimulus ringens floral displays often vary markedly in selfing rate. We hypothesized that this fine-scale variation in mating system reflects the tendency of bumble-bee pollinators to probe several flowers consecutively on multiflower displays. When a pollinator approaches a display, the first flower probed is likely to receive substantial outcross pollen. However, since pollen carryover in this species is limited, receipt of self pollen should increase rapidly for later flowers. Here the first direct experimental test of this hypothesis is described.

Methods

In order to link floral visitation sequences with selfing rates of individual flowers, replicate linear arrays were established, each composed of plants with unique genetic markers. This facilitated unambiguous assignment of paternity to all sampled progeny. A single wild bumble-bee was permitted to forage on each linear array, recording the order of floral visits on each display. Once fruits had matured, 120 fruits were harvested (four flowers from each of five floral displays in each of six arrays). Twenty-five seedlings from each fruit were genotyped and paternity was unambiguously assigned to all 3000 genotyped progeny.

Key Results

The order of pollinator probes on Mimulus floral displays strongly and significantly influenced selfing rates of individual fruits. Mean selfing rates increased from 21 % for initial probes to 78 % for the fourth flower probed on each display.

Conclusions

Striking among-flower differences in selfing rate result from increased deposition of geitonogamous (among-flower, within-display) self pollen as bumble-bees probe consecutive flowers on each floral display. The resulting heterogeneity in the genetic composition of sibships may influence seedling competition and the expression of inbreeding depression.

Key words: Autogamy, bee, Bombus fervidus, floral display, geitonogamy, mating system, monkeyflower, Mimulus ringens, paternity analysis, pollen carryover, pollinator visitation sequence, self-fertilization

INTRODUCTION

In many animal-pollinated Angiosperms, the proportion of seeds generated by self- and cross-fertilization varies dramatically among closely related species, among populations within species, and even among individuals within populations (see Barrett and Eckert, 1990; Barrett, 2003; Karron et al., 2004; Goodwillie et al., 2005; Medrano et al., 2005, and references therein). This variation has been attributed both to (a) plant traits, such as floral morphology (Humphreys and Gale, 1974; Epperson and Clegg, 1987; Motten and Antonovics, 1992; Karron et al., 1997; Herlihy and Eckert, 2007) and floral display size (Harder and Barrett, 1995; Snow et al., 1996; Karron et al., 2004; Williams, 2007); and (b) ecological factors, such as plant spatial distribution (Smyth and Hamrick, 1984; Warwick and Thompson, 1989; Karron et al., 1995a), pollinator identity and abundance (Brunet and Sweet, 2006), patterns of herbivory (Ivey and Carr, 2005) and the effects of competition for pollination (Bell et al., 2005). Selfing rates may even vary markedly on finer spatial scales (Barrett et al., 1994; Carromero and Hamrick, 2005). For example, studies of Mimulus ringens have documented striking variation in the mating system of adjacent flowers on the same daily floral display, often ranging from predominant selfing (s > 0·80) to predominant outcrossing (s < 0·20; Karron et al., 2004). These among-flower differences in the genetic composition of sibships increase the likelihood that self progeny will compete with other inbred siblings, potentially influencing the expression of inbreeding depression (Ritland, 1989; Schmitt and Ehrhardt, 1990). Although among-flower variation in mating patterns has important implications for theoretical research on the evolutionary stability of mixed mating systems (Holsinger, 1991; Lloyd, 1992; Goodwillie et al., 2005), the mechanisms responsible for this variation remain poorly understood.

We hypothesize that among-flower variation in selfing rates may reflect patterns of pollinator visitation. When a pollinator approaches a multiflower display (Fig. 1), the first flower probed receives outcross pollen and autogamous (within-flower) self pollen, but does not receive geitonogamous (among-flower) self pollen. However, if pollen carryover is limited, successive flowers probed on this display will receive increasing amounts of geitonogamous self pollen, resulting in increasing levels of self-fertilization (Barrett et al., 1994; Karron et al., 2004). Here the first direct experimental test of this hypothesis is described.

Fig. 1.
A bumble-bee approaching a floral display of Mimulus ringens.

The novel experimental design combines detailed pollinator observation with unambiguous assignment of paternity in order to quantify selfing rates of individual M. ringens fruits precisely. This species is especially appropriate for these studies because pollen carryover is very limited (Holmquist, 2005), increasing the likelihood that later flowers in the pollinator visitation sequence will primarily receive geitonogamous self pollen. The following questions are addressed. (a) Do selfing rates differ on the first, second, third and fourth flowers probed on multiflower displays? (b) Does the order of floral visitation influence the proportion of self-fertilization attributable to geitonogamy?

MATERIALS AND METHODS

Study species

Mimulus ringens (Phrymaceae) is a perennial herb native to wet meadows of central and eastern North America. Plants produce showy displays of large (2 cm) blue, zygomorphic flowers that are scattered across several indeterminate flowering stems. Each stem has only one or two open flowers. Flowers open simultaneously at dawn and last for half a day (Karron et al., 2004). Anthers dehisce and the stigma is receptive at the time of anthesis. At peak flowering, mean daily floral display size in five natural populations from north central USA ranges from 1·9 to 22·5 flowers, with an overall mean of 8·7 flowers (R. J. Mitchell, unpubl. res.).

At our field site in SE Wisconsin, USA, Mimulus flowers are primarily pollinated by workers of five bumble-bee species (Bombus fervidus, B. griseocollis, B. impatiens, B. nevadensis and B. vagans; Mitchell et al., 2004). The relative abundance of these pollinators fluctuates widely among years. During 2002, the year of the present study, B. fervidus was the principal pollinator of M. ringens.

When probing M. ringens flowers, bumble-bees contact the stigma and then the anthers with their tongues and faces (Mitchell et al., 2004). A single Bombus probe typically deposits 3000–7000 pollen grains (Flanagan et al., 2009) onto the papillose stigma. Nearly all flowers produce seed capsules (Karron et al., 2004). Each flower has approx. 6000 ovules, and seed set following a single Bombus probe is typically 1600–2300 seeds (Karron et al., 2006; Flanagan et al., 2009). Usually <400 of these seeds result from autogamous self-fertilization (Karron et al., 2004, 2006). Seed set for flowers receiving three probes is significantly (43·6 %) higher than seed set for flowers receiving a single probe (Karron et al., 2006). This suggests that stigmas often do not receive a saturating load of pollen during an initial probe.

Pollen carryover is often highly restricted in species pollinated by intensively grooming pollinators, such as Bombus (Thomson, 1986; Rademaker et al., 1997; Castellanos et al., 2003). In M. ringens, >90 % of the realized pollen dispersal from a donor flower is to the next three flowers in the visitation sequence (Holmquist, 2005). Despite this low level of pollen carryover, nearly all fruits are multiply sired (Mitchell et al., 2005). Flowers receiving a single probe average 3·12 outcross sires per fruit, indicating that pollen from multiple donors is often deposited at one time (Karron et al., 2006).

Propagation of genets with unique marker genotypes

In order to link floral visitation sequences with selfing rates of individual flowers, 20 replicate linear arrays were established, each composed of seven genets with unique multilocus combinations of homozygous genotypes at four unlinked allozyme loci [acid phosphatase (Acp-1, EC 3·1·3·2), aconitase (Aco-3, EC 4·2·1·3), glutamate oxaloacetate transaminase (Got-1, EC 2·6·1·1) and shikimate dehydrogenase (Skd-1, EC 1·1·1·25)]. These plants are a sub-set of 16 M. ringens genets that have been used extensively in previous paternity studies since they afford unambiguous assignment of paternity to all sampled offspring (Karron et al., 1995a, b, 1997, 2004, 2006). Each of these 140 plants (20 ramets of each of seven genets) was transplanted into 30 cm pots.

Experimental plot and linear arrays

The experimental plot was located at the University of Wisconsin-Milwaukee Field Station (Saukville, WI, USA). The plot contained a diversity of old field vegetation, including several species that are pollinated by bumble-bees. Since no other Mimulus plants were present and the nearest natural population is 15 km away, pots with flowering Mimulus plants were placed at this site so that bumble-bees would become familiar with the floral morphology of this species.

To ensure that flowers in each linear array were not visited prior to pollinator observations, all 140 Mimulus plants were placed into one of two pollinator-free screened enclosures on the evening prior to each observation day. These screened enclosures were also positioned on two opposite sides of the plot to increase the likelihood that bumble-bees would forage systematically along a linear array (similar to Morris et al., 1994).

Observation of foraging sequences

The linear arrays were set up on eight fair-weather days during August, 2002. At dawn, floral displays were trimmed to four flowers on six of seven genets in each array. The first plant in each array was not trimmed, and had 15–23 flowers. This larger display served to attract bees to the linear array. Ten linear arrays were sequentially exposed to pollinators from 0830 h to 1130 h each day. A single wild bumble-bee was permitted to forage on each array and the foraging sequence was videotaped using a Sony Video 8 Handycam with ×12 optical zoom. Immediately following the foraging sequence, the array was returned to the screenhouse to protect the flowers from further visitation.

If a bee failed to probe all four flowers on each genet sequentially, or if a second bee landed on the array, the foraging sequence was discarded. Videotaped foraging sequences were reviewed to verify that pollinators sequentially probed all four flowers on each genet, and to determine the order of floral visits on each display. In six of the 80 foraging sequences a single bee sequentially probed all four flowers on each genet. Each of these six foraging sequences were by B. fervidus workers.

Stigmas of the visited flowers closed within 90 min following pollination. Approximately 3 h following pollination, labelled plastic tags were tied to pedicels of each flower on genets three to seven. Flowers on the second plant in each array were not tagged because we wanted to ensure that flowers assessed for selfing rates had potentially received outcross pollen from at least two different donors. Once fruits had matured, all 120 fruits (4 flowers × 5 genets × 6 foraging sequences) were harvested.

Genotyping progeny

The 120 progeny arrays were germinated in separate 15 cm pots placed in a 20 °C plant growth chamber. When seedlings were 2 weeks old, they were transplanted into individual cells in plastic flats, and left to grow for an additional 3 weeks in a greenhouse. Twenty-five randomly selected seedlings from each progeny array were genotyped using the tissue extraction and electrophoretic methods of Karron et al. (2004). Seed germination rates were high (>80 %) and seedling mortality was minimal. In earlier work (J. D. Karron, R. J. Mitchell, K. G. Holmquist and J. M. Bell, unpubl. res.) no evidence was found for inbreeding depression at early stages of the life cycle. Therefore, it is unlikely that our selfing rate estimates are biased due to early mortality of inbred zygotes (Farris and Mitton, 1984).

Data analysis

Paternity was unambiguously assigned to all 3000 genotyped progeny, and we were therefore able to characterize selfing rates of each of the 120 fruits with a high degree of precision. Each fruit was classified as the first, second, third or fourth flower probed on a floral display. Sample sizes were equal (n = 30 fruits) in each class. Analysis of variance (ANOVA; JMP version 7.02; SAS Institute, 2007) was used to test for differences in selfing rate among the four visitation sequence classes, with individual ramets nested within bee foraging bouts. Bee foraging bout was treated as a random factor, and therefore its significance was tested over a denominator mean square of the sequence × bout interaction.

Selfing in the first flowers probed on each display is entirely attributable to autogamy. Assuming that the rate of autogamy is similar in the other three visitation sequence classes, the rate of geitonogamous selfing in these visitation classes can be estimated as follows: [geitonogamous selfing = (observed selfing rate) – (autogamous selfing rate in the first flowers probed)].

To quantify the proportion of self-fertilization attributable to geitonogamy, the ratio of geitonogamous selfing to total selfing in each visitation class was calculated.

RESULTS

The mean rate of self-fertilization varied significantly among the four visitation sequence classes (Table 1; Fig. 2). The mean selfing rate for initial probes (± s.e.) was 0·21 ± 0·03, for second flowers was 0·42 ± 0·03, for third flowers was 0·57 ± 0·04, and for fourth flowers was 0·78 ± 0·04 (Fig. 2). The selfing rate did not vary significantly among individual bee foraging bouts (Table 1; non-significant bout effect), and the effect of visitation sequence class was consistent across bouts (non-significant interaction). However, some ramets tended to have more selfing than others [significant ramet (bout) effect; ramet mean selfing rates varied from 0·25 to 0·88]. We could not directly test for effects of genet because genets were visited in the same order in every bout, therefore confounding genet identity with position in the foraging bout. However, there was relatively little difference in selfing rate among genet–position combinations (selfing rate means ranging from 0·34 to 0·59 across the five different categories of genet–position combination).

Fig. 2.
Effect of the pollinator visitation sequence on selfing rates in Mimulus ringens. Each data point shows the selfing rate for a single fruit, based on unambiguous assignment of paternity to 25 progeny per fruit. n = 30 for each visitation class.
Table 1.
Analysis of variance for the effects of visitation sequence class (whether the flower was the first, second, third or fourth sequentially probed flower on a plant) on selfing rate

Of the total among-fruit variation in selfing rate, 57 % was attributable to the sequence of floral visitation within displays, while 20 % was attributable to variation among ramets. On all 30 displays the selfing rate of the fourth flower probed was higher than the selfing rate of the first flower probed.

Estimated rates of geitonogamous selfing in the four visitation classes were: initial probes 0·00, second probes 0·21, third probes 0·37, fourth probes 0·57. Therefore, geitonogamous selfing accounted for nearly half of all selfing in second probes, and nearly three-quarters of all selfing in fourth probes.

DISCUSSION

The order of pollinator probes on M. ringens floral displays strongly and significantly influences selfing rates of individual fruits. Fruits resulting from initial probes are predominantly outcrossed, with a low rate of autogamous selfing. Since pollen carryover is very limited, successive flowers receive increasing amounts of geitonogamous self pollen. By the fourth flower probed, stigmas primarily receive geitonogamous self pollen, resulting in a high rate of self-fertilization. The implications of these findings are explored below.

Factors influencing among-flower variation in selfing rates

Most mating systems studies pool seeds of an entire plant, ignoring heterogeneity at finer spatial scales (Cruzan, 1998). However, Barrett et al. (1994) elegantly showed that selfing rates in Eichhornia paniculata increase from the bottom to the top of the indeterminate inflorescences. They attributed these patterns of selfing to bumble-bee behaviour, noting that bees tend to forage from the bottom to the top of an Eichhornia inflorescence. Our results build on this pioneering study and demonstrate that patterns of bumble-bee foraging directly influence the extent of geitonogamous self-fertilization in M. ringens. Three factors are especially likely to promote among-flower variation in selfing rates: (a) large floral display size; (2) limited pollen carryover; and (3) few successive probes to individual flowers.

The tendency of pollinators to probe several flowers in sequence on large daily floral displays (Klinkhamer et al., 1989; Robertson and Macnair, 1995; Goulson et al., 1998; Mitchell et al., 2004) leads to a positive relationship between floral display size and mean rate of self-fertilization (Harder and Barrett, 1995; Snow et al., 1996; Karron et al., 2004). Since sequentially probed flowers receive increasing amounts of geitonogamous self pollen, the among-flower variance in selfing on large displays is likely to be higher than the among-flower variance on small displays.

The extent of geitonogamous self-fertilization also strongly depends on patterns of pollen carryover (Robertson, 1992; Harder and Barrett, 1996). In M. ringens, most pollen from a donor flower is dispersed to the next three flowers in the visitation sequence (Holmquist, 2005). While the first flower probed may be largely outcrossed, the fourth flower probed may primarily receive geitonogamous self pollen. In contrast, in a species with more extensive pollen carryover, only a small fraction of the pollen deposited on the fourth flower will be geitonogamous self pollen. Therefore, species with extensive pollen carryover may exhibit little among-flower variation in selfing rate.

Accumulation of pollen on stigmas following several separate pollinator foraging bouts may also influence among-fruit differences in selfing rate (Dudash and Ritland, 1991; Mitchell et al., 2004). For example, a particular flower might be the first probed on the plant during one pollinator's visit (and therefore would receive mostly outcross pollen from this probe), and the last for a later pollinator (and therefore would receive mostly self pollen). Depending on the length of the interval between probes and how this interval affects paternity, the resulting fruit may have an intermediate selfing rate reflecting the weighted mean contribution of the two probes. Indeed, M. ringens stigmas often receive one or two additional probes within 15–60 min of the first (Karron et al., 2006), and pollen from these additional probes successfully fertilizes ovules (Karron et al., 2006). This provides an opportunity for later probes to even out the variation in selfing rate due to the order of the probe within displays. Yet selfing rates nonetheless vary markedly in open-pollinated M. ringens (Karron et al., 2004). One possible explanation for this surprising variation is that successive bees may probe flowers on a display in a similar order, and may therefore deposit similar proportions of self and outcross pollen onto stigmas (J. D. Thomson, University of Toronto, Toronto, pers. comm.)

Sibling competition and inbreeding depression

Wide among-flower variation in selfing within M. ringens floral displays has important implications for offspring fitness. Fruits of this species are gravity dispersed, and seeds frequently germinate in clusters likely to correspond to individual fruits. Although analyses of bulk seed samples may suggest that a plant has an intermediate selfing rate, the genetic composition of individual fruits may actually be highly heterogeneous. Since the expression of inbreeding depression may be influenced by seedling competition (Schmitt and Ehrhardt, 1990; Koelewijn, 2004), it may manifest very differently across sibships. Seedling size hierarchies are likely to be much more pronounced in fruits with mixtures of self and outcross progeny, and much less pronounced in fruits that only contain self progeny.

Conclusions

Adjacent flowers on M. ringens floral displays often vary markedly in patterns of self and outcross pollen deposition, leading to considerable heterogeneity in the genetic composition of sibships. This variation is largely attributable to the order of pollinator probes on floral displays. Although the first flower probed primarily receives outcross pollen, successive flowers receive increasing quantities of geitonogamous self pollen, leading to increasing rates of self-fertilization. Future research is needed to determine whether pollinator visitation patterns cause fine-scale variation in the mating system of other self-compatible Angiosperms, especially species with large floral displays and limited pollen carryover.

ACKNOWLEDGEMENTS

The authors thank Tom Schuck and Mindy Mymudes for assistance propagating Mimulus ringens, and Jim Reinartz, Gretchen Meyer and Lou Nelson for help with field work. We are grateful to Don Levin, Monica Medrano and an anonymous reviewer for providing helpful comments on an earlier version of the manuscript. This research was supported by NSF Grants DEB 9816712 to J.D.K., and DEB 9903308 to R.J.M., and a University of Wisconsin-Milwaukee Graduate School Fellowship to K.G.H.

LITERATURE CITED

  • Barrett SCH. Mating strategies in flowering plants: the outcrossing-selfing paradigm and beyond. Philosophical Transactions of the Royal Society B: Biological Sciences. 2003;358:991–1004. [PMC free article] [PubMed]
  • Barrett SCH, Eckert CG. Variation and evolution of mating systems in seed plants. In: Kawano S, editor. Biological approaches and evolutionary trends in plants. London: Academic Press; 1990. pp. 229–254.
  • Barrett SCH, Harder LD, Cole WW. Effects of flower number and position on self-fertilization in experimental populations of Eichhornia paniculata (Pontederiaceae) Functional Ecology. 1994;8:526–535.
  • Bell JM, Karron JD, Mitchell RJ. Interspecific competition for pollination lowers seed production and outcrossing rate in Mimulus ringens. Ecology. 2005;86:762–771.
  • Brunet J, Sweet HR. Impact of insect pollinator group and floral display size on outcrossing rate. Evolution. 2006;60:234–246. [PubMed]
  • Carromero W, Hamrick JL. The mating system of Verbascum thapsus (Scrophulariaceae): the effect of plant height. International Journal of Plant Sciences. 2005;166:979–983.
  • Castellanos MC, Wilson P, Thomson JD. Pollen transfer by hummingbirds and bumblebees and the divergence of pollination modes in Penstemon. Evolution. 2003;57:2742–2752. [PubMed]
  • Cruzan MB. Genetic markers in plant evolutionary ecology. Ecology. 1998;79:400–412.
  • Dudash MR, Ritland K. Multiple paternity and self-fertilization in relation to floral age in Mimulus guttatus (Scrophulariaceae) American Journal of Botany. 1991;78:1746–1753.
  • Epperson BK, Clegg MT. First-pollination primacy and pollen selection in the morning glory Ipomoea purpurea. Heredity. 1987;58:5–14.
  • Farris MA, Mitton JB. Population density, outcrossing rate, and heterozygote superiority in ponderosa pine. Evolution. 1984;38:1151–1154.
  • Flanagan RJ, Mitchell RJ, Knutowski D, Karron JD. Interspecific pollinator movements reduce pollen deposition and seed production in Mimulus ringens (Phrymaceae) American Journal of Botany. 2009;96:809–815. [PubMed]
  • Goodwillie C, Kalisz S, Eckert CG. The evolutionary enigma of mixed mating systems in plants: occurrence, theoretical explanations, and empirical evidence. Annual Review of Ecology and Systematics. 2005;36:47–79.
  • Goulson D, Stout JC, Hawson SA, Allen JA. Floral display size in comfrey, Symphytum officinale L. (Boraginaceae): relationships with visitation by three bumblebee species and subsequent seed set. Oecologia. 1998;113:502–508.
  • Harder LD, Barrett SCH. Mating costs of large floral displays in hermaphrodite plants. Nature. 1995;373:512–514.
  • Harder LD, Barrett SCH. Pollen dispersal and mating patterns in animal-pollinated plants. In: Lloyd DG, Barrett SCH, editors. Floral biology: studies on floral evolution in animal-pollinated plants. New York: Chapman and Hall; 1996. pp. 140–190.
  • Herlihy CR, Eckert CG. Evolutionary analysis of a key floral trait in Aquilegia canadensis (ranunculaceae): genetic variation in herkogamy and its effect on the mating system. Evolution. 2007;61:1661–74. [PubMed]
  • Holmquist KG. The effect of floral display and pollinator behavior on pollen-mediated gene dispersal in Mimulus ringens. Milwaukee, Wisconsin: University of Wisconsin-Milwaukee; 2005. PhD Thesis.
  • Holsinger KE. Mass–action models of plant mating systems: the evolutionary stability of mixed mating systems. American Naturalist. 1991;138:606–622.
  • Humphreys MO, Gale JS. Variation in wild populations of Papaver dubium. VII. The mating system. Heredity. 1974;33:33–42.
  • Ivey CT, Carr DE. Effects of herbivory and inbreeding on the pollinators and mating system of Mimulus guttatus (Phrymaceae) American Journal of Botany. 2005;92:1641–1649. [PubMed]
  • Karron JD, Thumser NN, Tucker R, Hessenauer AJ. The influence of population density on outcrossing rates in Mimulus ringens. Heredity. 1995;a 75:175–180.
  • Karron JD, Tucker R, Thumser NN, Reinartz JA. Comparison of pollinator flight movements and gene dispersal patterns in Mimulus ringens. Heredity. 1995;b 75:612–617.
  • Karron JD, Jackson RT, Thumser NN, Schlicht SL. Outcrossing rates of individual Mimulus ringens genets are correlated with anther–stigma separation. Heredity. 1997;79:365–370.
  • Karron JD, Mitchell RJ, Holmquist KG, Bell JM, Funk B. The influence of floral display size on selfing rates in Mimulus ringens. Heredity. 2004;92:242–248. [PubMed]
  • Karron JD, Mitchell RJ, Bell JM. Multiple pollinator visits to Mimulus ringens (Phrymaceae) flowers increase mate number and seed set within fruits. American Journal of Botany. 2006;93:1306–1312. [PubMed]
  • Klinkhamer PGL, de Jong TJ, de Bruyn GL. Plant size and pollinator visitation in Cynoglossum officinale. Oikos. 1989;54:201–204.
  • Koelewijn HP. Sibling competition, size variation and frequency-dependent outcrossing advantage in Plantago coronopus. Evolutionary Ecology. 2004;18:51–74.
  • Lloyd DG. Self- and cross-fertilization in plants II. The selection of self-fertilization. International Journal of Plant Sciences. 1992;153:370–380.
  • Medrano M, Herrera CM, Barrett SCH. Herkogamy and mating patterns in the self-compatible daffodil Narcissus longispathus. Annals of Botany. 2005;95:1105–1111. [PubMed]
  • Mitchell RJ, Karron JD, Holmquist KG, Bell JM. The influence of Mimulus ringens floral display size on pollinator visitation patterns. Functional Ecology. 2004;18:116–124.
  • Mitchell RJ, Karron JD, Holmquist KG, Bell JM. Patterns of multiple paternity in fruits of Mimulus ringens (Phrymaceae) American Journal of Botany. 2005;92:885–890. [PubMed]
  • Morris WF, Price MV, Waser NM, Thomson JD, Thomson BA, Stratton DA. Systematic increase in pollen carryover and its consequences for outcrossing in plant populations. Oikos. 1994;71:431–440.
  • Motten AF, Antonovics J. Determinants of outcrossing rate in a predominantly self-fertilizing weed, Datura stramonium (Solanaceae) American Journal of Botany. 1992;79:419–427. [PubMed]
  • Rademaker MC, de Jong TJ, Klinkhamer PGL. Pollen dynamics of bumble-bee visitation on Echium vulgare. Functional Ecology. 1997;11:554–563.
  • Ritland K. Correlated matings in the partial selfer Mimulus guttatus. Evolution. 1989;43:848–859.
  • Robertson AW. The relationship between floral display size, pollen carryover and geitonogamy in Myosotis colensoi (Kirk) Macbride (Boraginaceae) Biological Journal of the Linnean Society. 1992;46:333–349.
  • Robertson AW, Macnair MR. The effects of floral display size on pollinator service to individual flowers of Myosotis and Mimulus. Oikos. 1995;72:106–114.
  • SAS Institute Inc. JMP User's Guide. Cary, NC: SAS Institute Inc; 2007.
  • Schmitt J, Ehrhardt DW. Enhancement of inbreeding depression by dominance and suppression in Impatiens capensis. Evolution. 1990;44:269–278.
  • Smyth CA, Hamrick JL. Variation in estimates of outcrossing in musk thistle populations. Journal of Heredity. 1984;75:303–307.
  • Snow AA, Spira TP, Simpson R, Klips RA. The ecology of geitonogamous pollination. In: Lloyd DG, Barrett SCH, editors. Floral biology: studies on floral evolution in animal-pollinated plants. New York: Chapman and Hall; 1996. pp. 191–216.
  • Thomson JD. Pollen transport and deposition by bumble bees in Erythronium: influences of floral nectar and bee grooming. Journal of Ecology. 1986;74:329–341.
  • Warwick SI, Thompson BK. The mating system in sympatric populations of Carduus nutans, Carduus acanthoides and their hybrid swarms. Heredity. 1989;63:329–338.
  • Williams CF. Effects of floral display size and biparental inbreeding on outcrossing rates in Delphinium barbeyi (Ranunculaceae) American Journal of Botany. 2007;94:1696–1705. [PubMed]

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