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J Clin Microbiol. 1990 February; 28(2): 363–365.
PMCID: PMC269609

Effects of bovine serum albumin on the ability of Barbour-Stoenner-Kelly medium to detect Borrelia burgdorferi.


The ability of decreasing inocula of Borrelia burgdorferi to grow in otherwise identical Barbour-Stoenner-Kelly (BSK) media containing different lots of bovine serum albumin (fraction V) was determined. These media differed significantly in ability to detect B. burgdorferi. Some BSK media required inocula of 2 x 10(5) organisms per ml for detection, while other media could stimulate growth after inoculation with less than 2 organisms per ml. In addition, organisms from the less sensitive BSK media were thinner, longer, and less tightly coiled. The endpoint dilutions of indirect fluorescent-antibody titers, especially immunoglobulin M, exhibited up to 16-fold decreases, and both immunoglobulin G and M titers were more difficult to interpret with diagnostic slides prepared from some longer, thinner B. burgdorferi. These results demonstrate that, when performing laboratory investigations which rely on B. burgdorferi, it is essential that the quality of the BSK medium be determined.

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Selected References

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  • Anderson JF, Johnson RC, Magnarelli LA, Hyde FW. Identification of endemic foci of Lyme disease: isolation of Borrelia burgdorferi from feral rodents and ticks (Dermacentor variabilis). J Clin Microbiol. 1985 Jul;22(1):36–38. [PMC free article] [PubMed]
  • Anderson JF, Magnarelli LA, LeFebvre RB, Andreadis TG, McAninch JB, Perng GC, Johnson RC. Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas. J Clin Microbiol. 1989 Jan;27(1):13–20. [PMC free article] [PubMed]
  • Barbour AG. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984 Jul-Aug;57(4):521–525. [PMC free article] [PubMed]
  • Burgess EC. Natural exposure of Wisconsin dogs to the Lyme disease spirochete (Borrelia burgdorferi). Lab Anim Sci. 1986 Jun;36(3):288–290. [PubMed]
  • Callister SM, Agger WA, Schell RF, Brand KM. Efficacy of the urinary bladder for isolation of Borrelia burgdorferi from naturally infected, wild Peromyscus leucopus. J Clin Microbiol. 1989 Apr;27(4):773–774. [PMC free article] [PubMed]
  • Callister SM, Agger WA, Schell RF, Ellingson JL. Borrelia burgdorferi infection surrounding La Crosse, Wis. J Clin Microbiol. 1988 Dec;26(12):2632–2636. [PMC free article] [PubMed]
  • Godsey MS, Jr, Amundson TE, Burgess EC, Schell W, Davis JP, Kaslow R, Edelman R. Lyme disease ecology in Wisconsin: distribution and host preferences of Ixodes dammini, and prevalence of antibody to Borrelia burgdorferi in small mammals. Am J Trop Med Hyg. 1987 Jul;37(1):180–187. [PubMed]
  • Johnson RC, Kodner C, Russell M. In vitro and in vivo susceptibility of the Lyme disease spirochete, Borrelia burgdorferi, to four antimicrobial agents. Antimicrob Agents Chemother. 1987 Feb;31(2):164–167. [PMC free article] [PubMed]
  • Johnson RC, Marek N, Kodner C. Infection of Syrian hamsters with Lyme disease spirochetes. J Clin Microbiol. 1984 Dec;20(6):1099–1101. [PMC free article] [PubMed]
  • Levine JF, Wilson ML, Spielman A. Mice as reservoirs of the Lyme disease spirochete. Am J Trop Med Hyg. 1985 Mar;34(2):355–360. [PubMed]
  • Magnarelli LA, Anderson JF, Kaufmann AF, Lieberman LL, Whitney GD. Borreliosis in dogs from southern Connecticut. J Am Vet Med Assoc. 1985 May 1;186(9):955–959. [PubMed]
  • Russell H, Sampson JS, Schmid GP, Wilkinson HW, Plikaytis B. Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J Infect Dis. 1984 Mar;149(3):465–470. [PubMed]
  • Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE. The spirochetal etiology of Lyme disease. N Engl J Med. 1983 Mar 31;308(13):733–740. [PubMed]
  • Steere AC, Malawista SE, Snydman DR, Shope RE, Andiman WA, Ross MR, Steele FM. Lyme arthritis: an epidemic of oligoarticular arthritis in children and adults in three connecticut communities. Arthritis Rheum. 1977 Jan-Feb;20(1):7–17. [PubMed]

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