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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
From:
Vaccine. Author manuscript; available in PMC 2010 June 24.
Published in final edited form as:
Vaccine. 2009 June 24; 27(31): 4187–4195.
Published online 2009 May 9. doi: 10.1016/j.vaccine.2009.04.050

TABLE 3

Neutralizing antibody titers in ferrets vaccinated with inactivated whole-virus H5N1 influenza vaccine

Mean serum neutralizing antibody titer againsta
Vaccine regimenrg-A/VN/1203/04rg-A/HK/213/03rg-A/JWE/HK/1038/06rg-A/WS/MG/244/05

Nonadjuvanted vaccine1st dosec2nd dosec1st dosec2nd dosec1st dosec2nd dosec1st dosec2nd dosec
 rg-A/VN/1203/04b43 (3/3)90 (3/3)40 (2/3)242 (3/3)40 (3/3)56 (3/3)62 (3/3)95 (3/3)
 rg-A/HK/213/03b40 (1/3)44 (2/3)58 (3/3)653 (3/3)40 (1/3)71 (3/3)46 (3/3)170 (3/3)
 rg-A/JWE/HK/1038/06b40 (1/3)40 (2/3)40 (3/3)132 (3/3)43 (3/3)55 (3/3)69 (3/3)69 (3/3)
 Multiple-clade vaccineb43 (3/3)62 (3/3)178 (3/3)689 (3/3)60 (2/3)123 (3/3)122 (3/3)413 (3/3)

Adjuvanted vaccined
 rg-A/VN/1203/04b43 (6/8)1045 (9/9)60 (2/8)1073 (9/9)48 (5/8)214 (9/9)52 (8/8)511 (9/9)
 rg-A/HK/213/03b40 (4/9)100 (9/9)97 (9/9)3889 (9/9)40 (7/9)260 (9/9)53 (7/9)449 (9/9)
 rg-A/JWE/HK/1038/06b40 (1/9)70 (9/9)105 (7/9)1633 (9/9)82 (8/9)1648 (9/9)59 (8/9)255 (9/9)
 Multiple-clade vaccineb48 (2/9)343 (9/9)125 (9/9)2547 (9/9)61 (8/9)714 (9/9)74 (9/9)1707 (9/9)

Note: Titers for homologous vaccine and virus are underlined.

aExpressed as the reciprocal of the highest serum dilution that neutralized 50% of the HA activity of 100 TCID50 of virus infected cells. Results are the arithmetic mean titers of positive sera (titer ≥40). Number of positive sera/total is indicated in parentheses. Neutralizing antibody titers were measured for three ferrets per vaccine group.
bSingle-clade vaccines contained 7.5 μg HA per dose; multiple-clade vaccine contained 3.75 μg HA of each antigen per dose. Second (boost) dose was given 3 weeks after the initial dose.
cSerum samples were collected 3 weeks and 1 week, respectively, after the 1st and 2nd doses of vaccine.
dVaccination schedule and dosage for adjuvanted vaccines remained unchanged during the study so we averaged the results of the pre-challenge virus neutralizing antibody titers for ferrets in all challenge studies.