PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Am Soc Mass Spectrom. Author manuscript; available in PMC 2010 May 1.
Published in final edited form as:
PMCID: PMC2694139
NIHMSID: NIHMS114005

Gas-Phase Nazarov Cyclization of Protonated 2-Methoxy and 2-Hydroxychalcone: An Example of Intramolecular Proton-Transport Catalysis

Abstract

Upon CA, ESI generated [M + H]+ ions of chalcone (benzalacetophenone) and 3-phenyl-indanone both undergo losses of H2O, CO, and the elements of benzene. CA of the [M + H]+ ions of 2-methoxy and 2-hydroxychalcone, however, prompts instead a dominant loss of ketene. In addition, CA of the [M + H]+ ions of 2-methoxy-β-methylchalcone produces an analogous loss of methylketene instead. Furthermore, the [M + D]+ ion of 2-methoxychalcone upon CA eliminates only unlabeled ketene, and the resultant product, the [M + D - ketene]+ ion, yields only the benzyl-d1 cation upon CA. We propose that the 2-methoxy and 2-hydroxy (ortho) substituents facilitate a Nazarov cyclization to the corresponding protonated 3-aryl-indanones by mediating a critical proton transfer. The resultant protonated indanones then undergo a second proton transport catalysis facilitated by the same ortho substituents producing intermediates that eliminate ketene to yield 2-methoxy- or 2-hydroxyphenyl-phenyl-methylcarbocations, respectively. The basicity of the ortho substituent is important; for example, replacement of the ortho function with a chloro substituent does not provide an efficient catalyst for the proton transports. The Nazarov cyclization must compete with an alternate cyclization, driven by the protonated carbonyl group of the chalcone that results in losses of H2O and CO. The assisted proton transfer mediated by the ortho substituent shifts the competition in favor of the Nazarov cyclization. The proposed mechanisms for cyclization and fragmentation are supported by high-mass resolving power data, tandem mass spectra, deuterium labeling, and molecular orbital calculations.

Introduction

The acid-catalyzed cyclization of divinyl ketones to yield cyclopentenones is known as Nazarov cyclization, a reaction that was recently reviewed [1, 2]. Bronsted acids, superacids and Lewis acids are usually needed to promote the cyclizations in solution. The mechanism involves conrotatory electrocyclic ring closure of a protonated divinyl ketone followed by deprotonation and double bond reorganization [2]. A general and efficient method for the synthesis of biologically active 3-aryl-indanones is the Nazarov cyclization of substituted chalcones [3-6]. A variety of indanone derivatives can be synthesized by the microwave-assisted Nazarov cyclization of chalcones in trifluoroacetic acid (TFA) solution [5], In addition, further motivation comes from the antimicrobial activity of substituted chalcones, which was evaluated recently [7].

An external file that holds a picture, illustration, etc.
Object name is nihms114005u1.jpg

Characterization of these materials has attracted the attention of mass spectrometrists since the early 1960s [8]. The formation and structures of the [M - H]+ ion and [M − H - CO]+ ions from the M+, were one focus [9-14], including an ion-structure study with an ion-trap mass spectrometer [15]. Recently, the mechanisms for elimination of C6H6 and CO from the APCI-generated [M + H]+ ions of chalcones were established [16]. Three important product ions observed in the CAD mass spectrum of protonated chalcone arise from losses of H2O, CO, and C6H6. Studies of the substituted chalcones show that both of the phenyl rings are eliminated as the neutral arenes.

We took a different tack and describe here the possibility of conducting the Nazarov cyclization of chalcones in the gas-phase by using a mass spectrometer as both reactor and detector. Investigations of gas-phase reactions conducted using advanced experimental techniques in mass spectrometry and supported by theoretical calculations provide a means for understanding energetics and intrinsic mechanisms of reactions at the molecular level [17, 18]. Ionization methods such as CI and ESI are effective for protonating organic molecules while MS/MS and molecular modeling are widely used for the elucidation of gas-phase structures and fragmentation mechanisms. Proton-induced, gas-phase rearrangements may closely parallel those in solution as demonstrated by mass spectrometric studies; the acid-catalyzed Claisen rearrangement being a classic example [19]. A more recent example is the rearrangement of protonated 2-[N-benzoyloxyphenyl] benzamide both in the gas phase and in solution [20]. Another motivation for our study relies on evidence from both experiment and theory that high-energy 1,3-H shifts take place in the gas-phase when catalyzed by a base, a process called Proton Transport Catalysis [21-24]. The catalysis of the gaseous acetone radical cation enolization by benzonitrile [25, 26] and methanol [27] as well as the isomerizations of isoformyl/formyl cations by various neutrals [22] of ionized acetaldehyde by methanol are such examples [28].

We expected that ESI or CI methods protonate chalcones at the carbonyl oxygen, affording species that could potentially undergo Nazarov type cyclizations in the gas-phase. An important step in the solution Nazarov cyclization is deprotonation. A substituent such as OCH3 or OH at the ortho position may be sufficiently basic to cause deprotonation or otherwise assist in proton transport. Given that the OCH3 and OH groups are capable of catalyzing proton migrations in the gas-phase [27, 28], we chose to synthesize the following chalcones (substituted benzalacetophenones): chalcone (1), isomeric methoxy chalcones (2 and 5), isomeric hydroxy chalcones (4 and 6), 2-methoxy-β-methyl-chalcone (2-methoxybenzal-propiophenone) (3), and 2-chloro-chalcone (7) for this investigation. In addition, benzhydrols 8, 9 and 10 were synthesized and used to generate reference ions.

Experimental

Materials

Chalcones 1 to 7 were synthesized by standard procedures [9, 29, 30] with focus on various substituents at the 2- and 4- position of the ‘a’ ring. The benzhydrols used for generating product ions for comparison were synthesized by procedures already reported [31]. Purity of the samples was checked by TLC, and the structures confirmed by NMR, IR and mass spectra (Supplemental Materials). 3-phenyl-indanone was purchased form Aldrich Chemical Co. (Milwaukee, WI).

An external file that holds a picture, illustration, etc.
Object name is nihms114005u2.jpg

Mass spectrometry

Instrumental Methods

Formation of [M + H]+ ions was achieved by protonation of the chalcones by CI (chemical ionization) and ESI (electrospray ionization) methods. The [M + H]+ ions were analyzed by MS and tandem MS/MS and MS3 methods by using MI (metastable ion) or CA (collisional activation) under either low-energy (< 100 eV, laboratory) or high-energy (4 keV, laboratory) conditions.

The CI experiments, both MS and high-energy CA MS/MS, were conducted on a VG ZAB-T four-sector mass spectrometer (Manchester, UK) of BEBE design [32]. MS1 was a standard high-resolving power, double-focusing mass spectrometer (ZAB) of reverse geometry. MS2 possessed a prototype Mattauch-Herzog-type design, incorporating a standard magnet and a planar electrostatic analyzer having an inhomogeneous electric field, a single-point, and an array detector. Samples were introduced by evaporation from a direct-insertion probe; and ions formed were accelerated to 8 keV. The dissociation of the precursor ion (MI or CAD) was studied in the third field-free region. For CA, sufficient helium gas was added to the collision cell, which was floated at 4 kV, to decrease the main beam intensity by 30% for CAD experiments. Both MS1 and MS2 were operated at a mass resolving power of 1000. Typically 10-20 scans were signal averaged for each spectrum. Data acquisition and workup were accomplished by using a VAX 3100 workstation working with OPUS software.

The ESI-generated ions were produced from samples dissolved in 1:1 mixture of acetonitrile and water (10-20 μg/mL) and introduced by direct infusion at a flow rate of 10 μL/min for both MS and low-energy tandem MS analyses.

Some ESI and MS/MS experiments were conducted by using a Micromass Q-Tof-Ultima GLOBAL mass spectrometer (Manchester, UK) operated in the positive-ion mode. The needle voltage was 3 kV, and the cone voltage was 90 V. The temperatures of the source block and desolvation region were 90 and 150 °C, respectively. All parameters (i.e., aperture to the TOF, transport voltage, offset voltages) were optimized to achieve maximum sensitivity and a mass resolving power of 15,000 (full width at half maximum). The CAD experiments were carried out by mass selecting the precursor ion by using the quadruple analyzer, and the product ions were obtained by using the time-of-flight analyzer operated at a mass resolving power of 15000 (‘w’ mode). Collision voltages for fragmenting the ions were in the range of 7 to 10 volts with Ar as collision gas. Accurate masses of the product ions were determined by using the precursor ion as the internal standard.

Some ESI MS and low-energy MS/MS and tandem MS3 experiments were performed by using a Thermo Finnigan LCQ Advantage or a Thermo Finnigan LCQ Classic 3D ion-trap mass spectrometers (San Jose, CA).

Isotopic Labeling

To track fragmentation pathways, [M + D]+ ions were generated by using CD4 CI and were analyzed by high-energy CA and MI methods. In addition, [M + D]+ ions were generated by ESI from 1:1 D2O/acetonitrile mixture, introduced by direct infusion (10 μL/min) and analyzed by CA MS/MS and MS3 on the LCQ Classic ion trap

Theoretical Calculations

Owing to the large size of chalcones, many of the initial scans of the potential energy surfaces were performed by using the PM3 [33, 34] semi-empirical algorithm, where PM3 was part of the Spartan ’02 for Linux package (Wave function, Inc.). Further characterization was by density functional theory (DFT), which requires less computational overhead than do formal ab initio methods and yet incorporates dynamic correlation, has little spin contamination [35-37], and usually performs adequately giving proper geometries, energies, and frequencies [38]. DFT was part of the Gaussian 98 suite (Gaussian, Inc.) [39, 40]. Minima and transition states were optimized at the level B3LYP/6-31G(d, p) and confirmed by vibration frequency analysis. Connections of transition states to minima were analyzed by combination of inspection, projection along normal reaction coordinates, or reaction-paths calculations as needed; also discovered by the latter method were complexes (e.g. ion-dipole). Single-point energies were calculated at B3LYP/6-311+G(2d,p)//B3LYP-6-31G(d,p) level and scaled thermal-energy corrections for standard conditions were applied [41]. All calculated enthalpies are reported as relative to the initial protonated chalcone in kJ/mol.

Experimental Results and Discussion

Chalcone (1)

There are three major fragment ions in the CAD mass spectrum of the ESI-generated [M + H]+ (m/z 209) of chalcone. The fragments form via elimination of H2O, CO and C6H6 (presumably benzene) as reported earlier [19] along with minor fragments of m/z 103, m/z 105, presumably benzoyl cation, and m/z 194, from loss of the CH3 radical. A comparison of the CAD mass spectra of protonated chalcone and 3-phenylindanone (Table 1) reveals that both compounds give the same fragment ions (of m/z 194, 191, 181, 131 and 103). For the 3-phenylindanone case, however, the abundances of ions at m/z 191 and 181 are greatly reduced, indicating that these fragments originate from another ionic species. In addition, the absence of the m/z 105 ion suggests that the benzoyl cation seen in the CAD mass spectrum of protonated chalcone represents that fraction of [M + H]+ ions that does not cyclize. Nevertheless, the overall commonality of fragmentation suggests that some fraction of protonated chalcone isomerizes via Nazarov cyclization to afford protonated 3-phenylindanone.

Table 1
CAD mass spectra of ESI-produced [M + H]+ ions (quadrupole ion trap).

CA of the collision-generated m/z 181 ions ([M + H - CO]+ via MS3 experiments (Table 2) affords three important fragment ions of m/z 166, 153, and 103, which arise by elimination of CH3 , C2H4, and C6H6, respectively. The CAD mass spectra of the m/z 181fragment ions from protonated chalcone and 3-phenylindanone are similar, consistent with the proposed cyclization. In addition, CA of the m/z 181 ion obtained as [M + H - H2O]+ from protonated 1,1-diphenyl ethanol (10), selected as a suitable reference, exhibits the same fragments. The similarities indicate that the [M + H - CO]+ fragment ions from both protonated chalcone and 3-phenylindanone possess the 1,1-diphenylethylcation structure, Table 2.

Table 2
CAD mass spectra of the fragment ions of m/z 181.

2-Methoxychalcone (2)

The ESI-generated [M + H]+ ion of 2-methoxy chalcone (m/z 239) (2) fragments upon collisional activation to yield a dominant m/z 197 ion formed by elimination of 42 u (Table 1). The accurate mass of the fragment ion, 197.0965, corresponds to C14H13O (calculated mass = 197.0966), indicating that the expelled neutral is C2H2O, likely ketene. The dominant loss of ketene contrasts significantly with the fragmentations observed for protonated chalcone.

The metastable-ion (MI) decompositions of the CI-generated [M + H]+ of 2 (m/z 239) (Table 3) yield m/z 207, 197, 161, and 131 fragments formed by losses of methanol, ketene, and the elements of benzene and anisole, respectively. (Similar results were obtained for high-energy CAD of CI-generated [M + H]+ of 2.) We note that CI produces ions with greater internal energy than ESI, explaining the lack of m/z 207, 161 and 131 fragments in the ESI low-energy CAD mass spectrum (Table 1) and indicating that these latter ions are formed by higher energy processes than that giving the m/z 197 ion, which is likely produced by a low-energy rearrangement. High-energy CAD of the m/z 197 fragment ion affords fragments ions at m/z 181, 165, 152, and 91 (Fig. 1a). Given that the m/z 91 ion (benzyl or tropylium) corresponds to the base peak, the m/z 197 fragment must have a structure from which the C7H7 + cation can be readily generated.

Fig. 1
CAD mass spectra of (a) ion m/z 197 from 2 (b) m/z 197 ion from 3, (c) ion m/z 198 from [M + D]+ of 2. Instrument: BEBE tandem sector.
Table 3
Partial Metastable-ion (MI) mass spectra of CI produced [M + H]+ ions.

Furthermore, high-energy CAD of the [M + D]+ ion (m/z 240) of compound 2 generated by CD4 CI shows that [M + D]+ dissociates by eliminating ketene rather than ketene-d1, (m/z 198) indicating that the deuterium remains solely part of the product ion in the elimination of ketene. High-energy CA of the m/z 197 ion (Fig. 1b) yields major fragments of m/z 181 (due to loss of CH4), 165 (loss of CH3OH), and 91 (formation of C7H7 +), which are all shifted upward by one m/z upon CA of the m/z 198 ion (Fig. 1c). These results suggest that the initial D of m/z 240 has become one of the aromatic protons of the m/z 198 ion, specifically on the unsubstituted phenyl ring. We postulate that the likely structure of the m/z 197 ion is that of the 2-methoxyphenyl-phenyl-methyl cation and the C7H7 + is consequently the benzyl cation.

We generated a suitable reference for the m/z 197 ion having the 2-methoxyphenyl-phenyl-methyl cation structure by low-energy CA of the ESI-generated [M + Na]+ ion of 2-methoxybenzhydrol (8). Others have reported generating 2-methoxyphenyl-phenyl-methyl cation by loss of H2O from the CI-generated [M + H]+ ion of 8 and producting its CAD mass spectrum via an ion trap instrument, which shows an abundant fragment of m/z 91 (benzyl cation) [42]. Furthermore, they reported an C7H5D2 + ion as a CAD fragment of the OCD3 analog (m/z 200), derived by the loss of H2O from protonated 2-d3-methoxybenzhydrol, thus indicating that the methylene moiety of the benzyl cation is derived from the OCH3 group.

Low-energy CA (Fig. 2a) of the collision-generated m/z 197 fragment via an MS3 experiment involving 2-methoxychalcone (2) compares well with that of the m/z 197 ion (MS3 experiment) from compound 8 (Fig. 2b). The strong similarity indicates that the [M + H − CH2CO]+ ion from compound 2 is the 2-methoxyphenyl-phenyl-methyl cation (Equation 1). Low energy CA in an MS3 experiment involving the m/z 197 ion (Fig. 2a) causes losses of H2O and CO (forming m/z 179 and 169 ions, respectively) and formation of the m/z 91 ion. The m/z of these fragments increases by one for the m/z 198 intermediate [M + D - CH2CO]+ (Fig. 2c). Similar product-ion m/z shifts occur upon high-energy CA of the CI-produced m/z 198 ion (Fig. 1c). The facile losses of H2O and CO likely indicate substantial but low-energy rearrangement processes, whereas the losses of CH4 and CH3OH (Fig. 1a) induced by high-energy MS/MS experiments are more direct, higher energy processes. In either case, the production of the m/z 91 ion is a dominant process exhibiting identical deuterium-labeling results.

Fig. 2
CAD mass spectra of (a) m/z 197 from 2-methoxy chalcone (2), (b) m/z 197 from 2-methoxybenzhydrol (8), (c) m/z 198 from [M + D]+ of 2. (All are MS3 experiments). Instrument: 3D Ion Trap
equation image
Equation 1

2-Methoxy-β-methyl-chalcone (3)

We chose to examine the CAD mass spectrum of the ESI-produced [M + H]+ ion of β-methyl analogue, 2-methoxy-β-methyl-chalcone (3) to determine the origin of the ketene. The [M + H]+ expectedly dissociates via elimination of methylketene to afford the m/z 197 ion (Table 1). Its accurate mass is 197.0964, in good agreement with the calculated mass of [M + H - CH3CH=C=O]+. The other major fragment ion is [M + H - H2O] + (measured mass, 235.1129; calculated for C17H15O, 235.1123), which is likely facilitated by the protons on the β-methyl group.

The MI and high-energy CAD mass spectra of the CI-generated [M + H]+ of 3 (Table 3) exhibit peaks corresponding to m/z 235, 197, and 145 ions, formed by expulsions of H2O, methyl ketene (56 u) and CH3OC6H5 (108 u), respectively. Moreover, CA of the m/z 197 ion obtained by CI protonation of compounds 2 and 3 (Fig. 1a,b) gives similar results, indicating that the m/z 197 fragments from both compounds have the same structure, namely, the 2-methoxyphenyl(phenyl)methyl cation formed by similar reaction (Equation 2). These observations indicate that the [M + H]+ of compound 3 eliminates methylketene by a mechanism analogous to that for loss of ketene from protonated 2 and that the β carbon along with the adjacent carbonyl group form the ketene core.

equation image
Equation 2

2-Hydroxy-chalcone (4)

To delineate the role of methoxy in the elimination of ketene, we replaced the ortho OCH3 by OH and protonated the 2-hydroxy-chalcone by CI and ESI. The MI dissociations of the CI-generated [M + H]+ (m/z 225) are losses of H2O, ketene, and C6H6 (giving m/z 207, 183, and 147 ions, respectively) (Table 3). CA of the ESI-generated [M + H]+ (Table 1) causes ketene elimination and formation of the benzoyl cation at m/z 105; the presence of the highly abundant m/z 183 ion and the absence of the m/z 207 and 147 ions suggest that loss of ketene is a lower energy process than losses of H2O and C6H6, as is also the case for 2-methoxy-chalcone (2).

By analogy to the structure of the [M + H − CH2CO]+ fragment from 2-methoxy chalcone, we propose that the m/z 183 fragment is the 2-hydroxyphenyl-phenyl-methyl cation (Equation 3). As a reference, we synthesized 2-hydroxybenzhydrol (9) that, upon protonation by ESI followed by CA, loses water to give the desired m/z 183 fragment. Low-energy CA of the ion from 6 (Fig. 3a) and from its reference 9 (Fig. 3b, Table 4 MS3) gives similar spectra, indicating that the two m/z 183 ions have the 2-hydroxyphenyl-phenyl-methyl cation structure [43]. Hence, the elimination of ketene from 4 follows a mechanism analogous to that for its elimination from 2, indicating that the OH group plays a nearly identical role as OCH3 in elimination of ketene.

Fig 3
a: The CAD MS of fragment ion of m/z 183 obtained by MS3 experiment on the ESI produced [M + H]+ of compound 5.
Table 4
CAD mass spectra of collisionally generated ions of m/z 183.
equation image
Equation 3

The [M + H]+ of 4 has two OH groups (assuming protonation on the carbonyl oxygen), and hence H/D exchange/scrambling is possible in the corresponding [M + D]+ ion so that the D may be present as phenolic OD for some fraction of the [M + D]+. Given that H+-shifts can be mediated by the oxygen atom of the phenolic group, both retention and loss of deuterium accompanying formation of the product ions are expected. High-energy CA of CI-generated [M + D]+ of m/z 226 from 4 does indeed produce m/z 183 and 184 ions in the abundance ratio of ~ 1:2, confirming that H/D exchange occurs and that both ketene and ketene-d1 are eliminated. CA of ESI-generated [M −H + 2D]+ ion of m/z 227 from 4 (Fig. 4) gives m/z 183, 184, and 185 ions owing to eliminations of ketene-d2, ketene-d, and ketene in ratio of ~1:3:1, indicating H/D scrambling prior to ketene elimination. The formation of ketene-d2 along with the ratios of deuteria in the ketene elimination products indicate that, in addition to the two hydroxyl hydrogens, two other hydrogen are involved in scrambling. The proton of the ortho OH group must somehow initiate H/D scrambling, something that the otherwise similar ortho OCH3 (from 2) cannot.

Fig.4
CAD mass spectrum of [2-hydroxychalcone – OH + 2D]+. Instrument: 3D Ion Trap

In summary, protonated 2-methoxy (2) or 2-hydroxychalcone (4) precursors decompose predominantly upon CA by ketene elimination to give 2-methoxy or 2-hydroxyphenyl-phenyl-methyl cations, respectively. This process requires a 1,3-migration or equivalent of the unsubstituted phenyl from the carbonyl carbon to the α carbon. Furthermore, the loss of methylketene from protonated 2-methoxy-β-chalcone (3) implies that the β-olefinic carbon, adjacent to the carbonyl group, is eliminated as part of the ketene in these systems. Finally, fragmentation of the [M + D]+ of (2) via ketene loss proceeds with complete retention of the D on the product m/z 198 ion, which fragments to yield benzyl ion (m/z 92) also with D retention, implying that the initial D is transferred from the presumptive site of charging, the carbonyl oxygen, to an aryl site on the unsubstituted phenyl ‘b’ ring. To accommodate these three mechanistic criteria, we propose that the protonated 2-methoxy and 2-hydroxy-chalcones undergo, upon activation, Nazarov cyclizations to give protonated 3-aryl-indanones from which ketene is eliminated (Equation 4). In addition, the 2-methoxy/2-hydroxy groups must play a crucial role for both the Nazarov cyclization and ketene loss because the unsubstituted chalcone, when protonated, gives only partial cyclization with no observable ketene loss.

equation image
Equation 4

2-Chlorochalcone (7)

We designed a test for the hypothesis by investigating a chalcone with a less basic ortho substituent to delineate better the requirements for the cyclization and loss of ketene. CA of the [M + H]+ of 2-chlorochalcone shows that it fragments to yield m/z 225, 215, 165, and 131 ions by eliminations of H2O, CO, C6H6, and C6H5Cl, respectively, analogous to the fragmentations of chalcone (Table 1). The m/z 201 fragment produced by ketene elimination, however, is only 2%, indicating that the poorly basic Cl group is less capable than OCH3 and OH in catalyzing cyclization and ketene elimination. Hence, there seems to be a minimum basicity requirement for the promotion of cyclization and the elimination of ketene. In addition, the low abundances of benzoyl cation (4%) and of fragments arising from elimination of H2O or CO suggest that some fraction of the protonated 7 cyclizes by analogy to protonated chalcone.

4-Methoxychalcone (5) and 4-Hydroxychalcone(6)

To explore other criteria for cyclization, we examined 4-methoxychalcone (5) and 4-hydroxychalcone (6), using CI and ESI to protonate the neutral molecules. The MI dissociations of the CI-generated [M + H]+ from 5 and 6 give rise to not only the benzoyl cation but also other fragments formed by eliminations of H2O, C6H6, and substituted C6H6 analogous to eliminations from protonated unsubstituted chalcone; no detectable elimination of ketene occurs. CA of the ESI-produced [M + H]+ ions also produces benzoyl cations and other fragment ions formed by the eliminations of H2O, CO and C6H6, and substituted C6H6 as established by accurate mass measurements (Table 5), but again no detectable elimination of ketene. By analogy to unsubstituted chalcone, a small fraction of protonated 5 and 6 may also cyclize to substituted indanones; the lower abundance of the benzoyl cation generated when ESI was used for protonation compared to when CI was used and MI spectra taken (Table 1 and and3)3) indicates that cyclization takes place to a greater extent upon protonation by ESI. Overall, the results for protonated 5 and 6 relative to 2 and 4 clearly demonstrate the necessity of the ortho substitution for the promotion of Nazarov cyclization and subsequent elimination of ketene.

Table 5
Accurate masses of the fragment ions of compounds 5 and 6.

Proposed mechanisms and theoretical calculations

Theory: Protonated Chalcone

We undertook theoretical calculations (Experimental: theoretical calculations) to aid in the elucidation of fragmentation mechanisms and the role of cyclization. Specific subjects are protonation, feasibility of cyclization, and subsequent fragmentation of protonated chalcone (1), 2-methoxy-chalcone (2) and 2-hydroxy-chalcone (9). Calculations reveal that the lowest-energy protonation of [M + H]+ ions occurs on carbonyl oxygen in all three cases. We chose these initial forms, A1, as the reference points for calculating relative enthalpies of formation and reaction in Tables 6,,77.

Table 6
Calculated relative enthalpies of formation/reaction (Scheme 1, in kJ/mol).
Table 7
Calculated relative enthalpies of formation and reaction (Scheme 2, in kJ/mol).

In addition to A1, there is an ensemble of other uncyclized forms related by rotations about various bonds between the two phenyl rings and by proton transfers (Ai of the Schemes). The formation of protonated 3-aryl-1-indanones from these precursors via Nazarov cyclization in the gas phase would require the equivalent of a 1,3-H+ transfer after the conrotatory cyclization. We could not find this transition state and, even if it did exist, it probably would require substantial energy to cross such that other processes would predominate. As shown on Scheme 1, there are three other routes (Fig. 5) to accomplish that equivalent of this H-transfer. Route 1, A1A2A3B1B2C3C6, involves a pair of 1,2-H+ transfers, however, the transition state TS(B1-B2) still requires > 250 kJ/mol additional energy to surmount (Table 6). On Route 2, A1A4C1C2C3C6 which requires a pair of 1,4-H+ transfers, the greatest barrier is transition state TS(C1-C2) which requires 200-220 kJ/mol additional energy. Route 3, A1A4A5C4C5C6 requires a pair of favorable 1,5-H+ transfers involving the ortho substituent and, in contrast, presents maximum barriers on trajectory from A5 to C6 that require < 160 kJ/mol additional energy. In addition, calculations reveal that the protonated 3-aryl-1-indanones are the most stable structures on the potential energy surface from protonated chalcones to the elimination of ketene.

Fig. 5
Comparative enthalpies of formation along reaction routes to protonated 3-aryl indanone formation (2-methyoxychalcone case).
Scheme 1
Proposed mechanism, formation of protonated 3-aryl-indanone from protonated chalcone precursor.

Route 3, the most favorable route through cyclization to the protonated 3-aryl-1-indanones (Fig. 5 illustrating 2-methyoxychalcone case), constitutes an example of intramolecular proton-catalyzed transport (21-24) where the transport mediator is the oxygen of the 2-hydroxy or 2-methoxy groups. Since Route 3 in not available for the unsubstituted chalcone case, conversion to protonated 3-aryl-1-indanone then would be by Route 2 requiring 204 kJ/mol, hence cyclization for the unsubstituted chalcone would be less competitive and only partially complete, as observed experimentally. The most favorable Route 3 would also not be available for the 4-hydoxy and 4-methoxy isomers for geometric reasons, explaining why these isomers exhibit similar fragmentation features as the unsubstituted chalcone. A critical requirement for intramolecular proton-catalyzed transport of Route 3 is the presence of an ortho substituent of sufficient basicity to abstract a proton from the C9 (former C2’) position for subsequent transfer to the C2 (former β) position. The calculated relative enthalpies of formation for C4, C5, and connecting transition state TS(C4-C5) show additional investment < 25kJ/mol to accomplish a feasible proton transfer to the 2-hydroxy and 2-methoxy moieties (Table 6). In contrast, C5 for the 2-chloro substituent is not stable, and thus would be less efficient as an agent for proton-catalyzed transport [22].

The elimination of ketene from the protonated 3-aryl-1-indanones (e.g. from (2) and (4)), requires the translocation of the proton on the carbonyl oxygen to somewhere on the phenyl ring (former ‘b’ ring), as required by the D labeling results, particularly in the 2-methoxy case (2) where no d1-ketene loss occurs. Two routes are available (Scheme 2, Fig. 6). Route A involves transfer of proton from the carbonyl oxygen to C7 and then to C8, C6C8C9G1 where TS(C8-C9) requires > 210 kJ/mol additional energy to surmount. In contrast, route B, C6C7G1 presents a maximum barrier of`~130 kJ/mol to same intermediate (G1). On route B, the OCH3 and OH groups act as intramolecular proton transfer catalysts; the ionizing H+ on the carbonyl group is first abstracted to the 2-methoxy or 2-hydroxyl group and then transferred to the C8 position (former C1’ on the ‘b’ ring), activating the adjacent C-C bond for cleavage to G2. The basicity of the ortho OCH3 and OH groups is necessary for efficient proton transfer catalysis (Table 7), something that an ortho Cl cannot perform because corresponding intermediate C7 is unstable. From G2 are two energetically similar paths, one direct and another through a cyclic intermediate G3, resulting in formation an ion-dipole complex, IDCK, preparatory to elimination of ketene. The ionizing proton thus is translocated to the ortho position of the unsubstituted phenyl group in the product ion H1, in accord with D+ labeling results The ketene loss via proton transport catalysis is so facile that it suppresses any other fragmentations routes for the protonated 3-(2-methoxy) phenyl-1-indanone intermediate, whereas ketene loss is not observed for protonated 3-phenyl-1-indanone.

Fig. 6
Comparative enthalpies of formation/reaction of ketene elimination from protonated 3-aryl indanone (2-methoxychalcone case).
Scheme 2
proposed mechanism, elimination of ketene from protonated 3-aryl-1-indanone.

The 2-hydroxy analog (4) differs from the 2-methoxy case in several important ways. First, there is a much greater abundance of the m/z 105 ion in the ESI CAD mass spectrum of 4 (Table 1) compared to that of 2-methoxychalcone under identical conditions (same instrument and collision energy). Second, H/D scrambling occurs in both high-energy CAD of the CI-produced [M + D]+ and low-energy CAD of the ESI-produced [M − H + 2D]+ whereas no H/D exchange is detectable for the 2-methoxy analogue (2). We rationalize these differences by a proposed mechanism (Scheme 3) whereby the protons of the 2-OH groups and the β-C-H site are interchanged becoming equivalent with regard to H/D labeling. This interchange is initiated by the phenol OH, which is not possible for the 2-methoxy analogue. A fourth proton, at C2’ of the ‘b’ ring (C9 after cyclization), becomes involved in that both protons on the R–OH2 + moiety of intermediate C5 (Scheme 1) can be transferred to the C6 site (former β-C) by virtually equal barriers, TS(C5-C6) vs TS(C5-C6)u (Table 6). This process is also unavailable to the 2-methoxy analogue. The resulting H/D distribution would be 1:2 for d1/d0-ketene elimination from [M + D]+, as is observed, and 1:4:1 for d2/d1/d0-ketene from [M − H + 2D]+, which is close to the experimental ~1:3:1. Thus, the labeling results provide strong confirmation for the proposed mechanisms. In addition, generation of m/z 105, the benzoyl cation, is accomplished by direct cleavage of A8 with no reverse activation barrier.

Scheme 3
Proposed mechanism involved in H/D scrambling in protonated 2-hydroxy-chalcone.

Another type of cyclization is possible where the protonated carbonyl initiates an electrophilic attack upon the ‘a’ ring (Scheme 4). The cyclic products thus generated are configured for ready elimination of H2O. This route of elimination, however, requires greater energy than that for ketene elimination and appears to be competitive only for those compounds that do not have an ortho substituent to catalyze critical proton-catalyzed transports.

Scheme 4
Proposed alternate cyclization of protonated chalcone (1).

From the [M + D]+ of 2-methyoxy-chalcone (2), the quantitative formation of the m/z 92 vs. m/z 91 (Fig. 2c) product ion from the collision-generated m/z 198 ion [M + D − CH2CO]+ clearly substantiates that D is present in the phenyl ring of m/z 198. This reaction belongs to the class of empirically-observed fragmentations from substituted diphenylcarbinols and diphenylmethyl cations [42,43]. We have verified the basic postulated mechanism by theoretical calculations; the m/z 197 ion decomposes to give the m/z 91 ion benzyl cation formed by the mechanism in Scheme 5. An addition to the original proposed mechanism revealed by theoretical calculations is the formation of an ion-dipole complex, IDCQ, in the fragmentation exit channel to products. The mechanism preserves the location of the charging proton at the ortho position of the unsubstituted phenyl ring where it is remote to exchange reactions.

Scheme 5
Proposed mechanism, from m/z 197 to m/z 91 [42,43]. Calculated enthalpies reported relative to H1 in kJ/mol.

Further information regarding the theoretical calculations is available in the Supplementary Materials.

Conclusion

Although a Nazarov-type cyclization likely occurs to some extent for protonated chalcone, this process becomes of high yield for the protonated 2-hydroxy and 2-methoxy chalcones (Scheme 1). The intermediate products are protonated 3-aryl-1-indanones. A key finding is that OCH3 and OH groups at the ortho position act as proton-transfer catalysts, in that they decrease the activation energy for the equivalent 1,3 proton shift, a key step. Subsequently, ketene elimination occurs by a second, key 1,3 proton-shift (Scheme 2) similarly catalyzed by the oxygen atom of the ortho OCH3 or OH groups. When the OCH3 or OH groups, however, are absent from the ortho position, Nazarov-type cyclization takes place to a significantly lesser extent, and must compete with another type of cyclization. In addition, competitive expulsions of CO, H2O and C6H6 (or substituted C6H6) take place instead of ketene which are also characteristic of protonated 3-phenyl-1-indanone. The structures of the [M + H - ketene]+ and [M + H - CO]+, as determined by comparison of their CAD mass spectra with those of reference ions, are consistent with these hypotheses. The proposed mechanisms find strong support from theoretical calculations using density functional theory and from accurate-mass data, tandem mass spectrometric experiments, and deuterium-labeling.

The gas-phase Nazarov cyclization of protonated chalcones is analogous to that occurring in solution. Its extent in the gas phase depends on the method used to protonate the starting material and on the nature and position of substituents. Moreover, the study shows that the cyclization and fragmentation of 2-methoxy and 2-hydroxy-chalocone are examples of intramolecular proton-transport catalysis.

Supplementary Material

01

Acknowledgments

V.S.S. and M.G. thank the KSCSTE for financial assistance and Principal, S. H. College, Thevara for providing infrastructure. R.S. thanks Dr. J. S. Yadav, Director, IICT, Hyderabad, for facilities and Dr. M. Vairamani for cooperation. Research at WU was supported by the National Centers for Research Resources of the NIH, Grant P41RR00954. In addition, this work made use of the Washington University Computational Chemistry Facility, supported by NSF grant #CHE-0443501.

Footnotes

Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Contributor Information

M. George, Department of Chemistry, Sacred Heart College, Thevara, Cochin, Kerala, India-682013.

V. S. Sebastian, Department of Chemistry, Sacred Heart College, Thevara, Cochin, Kerala, India-682013.

P. Nagi Reddy, National center for Mass Spectrometry, IICT, Hyderabad, India.

R. Srinivas, National center for Mass Spectrometry, IICT, Hyderabad, India.

Daryl Giblin, Department of Chemistry, Washington University in St.Louis, St.Louis, USA, MO 63130.

Michael L. Gross, Department of Chemistry, Washington University in St.Louis, St.Louis, USA, MO 63130.

References

1. Pellissier H. Recent developments in the Nazarov process. Tetrahedron. 2005;61:6479–6517.
2. Frontier A, Collinson C. The Nazarov cyclization in organic synthesis. Recent advances. Tetrahedron. 2005;61:7577–7606.
3. Gu XH, Yu H, Jacoboson AE, Rothman RB, Dersch CM, George C, Flippen-Anderson JL, Rice KC. Design, Synthesis, and Monoamine Transporter Binding Site Affinities of Methoxy Derivatives of Indatraline. J Med Chem. 2000;43:4868–4876. [PubMed]
4. Elliott JD, Lago MA, Cousins RD, Leber JD, Erhard KF, Nambi P, Elshourbagy MA, Kumar C, Lee JA, Bean JW, DeBrosse CW, Eggleston DS, Brooks DP, Geuerstein G, Ruffolo RR, Jr, Weinstock J, Gleason JG, Peishoff CE, Ohlstein EH. 1,3-Diarylindan-2-carboxylic Acids, Potent and Selective Non-Peptide Endothelin Receptor Antagonists. J Med Chem. 1994;37:1553–1557. [PubMed]
5. Lawrence NJ, Simon E, Armitage M, Greedy B, Cook D, Ducki S, McGown AT. The synthesis of indanones related to combretastatin A-4 via microwave-assisted Nazarov cyclization of chalcones. Tetrahedron Letters. 2006;47:1637–1640.
6. Yin W, Ma Y, Xu J, Zhao Y. Microwave-assisted one-pot synthesis of 1-indanones from arenes and α,β-unsaturated acyl chlorides. J Org Chem. 2006;71:4312–4315. [PubMed]
7. Sivakumar PM, Seenivasan SP, Kumar V, Doble M. Synthesis, antimycobacterial activity evaluation, and QSAR studies of chalcone derivatives. Bioorg Med Chem Lett. 2007;17(6):1695–1700. [PubMed]
8. Beynon JH, Lester GR, Williams AE. Specific molecular rearrangements in the mass spectra of organic compounds. J Phy Chem. 1959;63:1861–8.
9. Van De Sande C, Serum J, Vandwalle W. Organic mass spectrometry. XII. Mass spectra of chalcones and flavanones. Isomerization of 2’-hydroxychalcone and flavanone. Org Mass Spectrom. 1972;6:1333–46.
10. Ronayne J, Williams DH, Bowie JH. Mass spectrometry. XIX. Evidence for the occurrence of aromatic substitution reactions upon electron impact. J Am Chem Soc. 1966;88:4980–4.
11. Rouvier E, Medina H, Cambon A. Mass spectrometric studies. VIII. Fragmentation of several benzalacetophenones and benzalacetones variously substituted on the aromatic ring. Org Mass Spectrom. 1976;11:800–13.
12. Schaldach B, Grutzmacher HF. The fragmentations of substituted cinnamic acids after electron impact. Org Mass Spectrom. 1980;15(4):175–81.
13. Kallury RKMR, Loudon AG, Maccoll A. Electron impact studies on α, β -unsaturated carbonyl oximes. Org Mass Spectrom. 1978;13(4):218–23.
14. Ardanaz CE, Kavka J, Curcuruto O, Traldi P, Guidugli F. On the structure of [C9H6O]+. ions originating by electron impact induced decomposition of chalcone. Rapid Commun Mass Spectrom. 1991;5(11):569–73.
15. Ardanaz CE, Traldi P, Vettori U, Kavka J, Guidugli F. The ion-trap mass spectrometer in ion structure studies. The case of [M-H]+ ions from chalcone. Rapid Commun Mass Spectrom. 1991;5:5–10.
16. Tai Y, Pei S, Wan J, Cao X, Pan Y. Fragmentation study of protonated chalcones by atmospheric pressure chemical ionization and tandem mass spectrometry. Rapid Commun Mass Spectrom. 2006;20:994–1000. [PubMed]
17. Bohme DK, Schwarz H. Gas-phase catalysis by atomic and cluster metal ions: the ultimate single-site catalysts. Angew Chem Int Ed. 2005;44:2336–2354. [PubMed]
18. O’Hair RAJ. The 3D quadrupole ion trap mass spectrometer as a complete chemical laboratory for fundamental gas-phase studies of metal mediated chemistry. Chem Commun. 2006;14:1469–1481. [PubMed]
19. Kingston EE, Beynon JH, Liehr JG, Meyrant P, Flammang R, Maquestiau A. The Claisen rearrangement of protonated allyl phenyl ether. Org Mass Spectrom. 1985;20:351–359.
20. Moolayil JT, George M, Srinivas R, Swamy NS, Russell A, Giblin D, Gross ML. The mass spectrometry-induced cyclization of protonated N-[2-(benzoyloxy)phenyl]-benzamide: A gas-phase analog of a solution reaction. Int J mass Spectrom. 2006:249–250. 21–30.
21. Bohme DK. Proton transport in the catalyzed gas-phase isomerization of protonated molecules. Int J Mass Spectrom Ion Processes. 1992;115:95–110.
22. Chalk AJ, Radom L. Proton-Transport Catalysis: A systematic Study of the Rearrangement of the Isoformyl Cation to the Formyl Cation. J Am Chem Soc. 1997;119:7573–7578.
23. Chalk AJ, Radom L. Ion-Transport Catalysis: Catalyzed Isomerizations of NNH+ and NNCH3+ J Am Chem Soc. 1999;121:1574–81.
24. Ruttink PJA, Burgers PC, Fell LM, Terlouw JK. Dissociation of Ionized 1,2-Ethanediol and 1,2-Propanediol: Proton-Transport Catalysis with Electron Transfer. J Phys Chem. 1998;102:2976–80.
25. Trikoupis MA, Terlouw JK, Burgers PC. Enolization of Gaseous Acetone Radical Cations: Catalysis by a Single Base Molecule. J Am Chem Soc. 1998;120(46):12131–32.
26. Trikoupis MA, Burgers PC, Ruttink PJA, Terlouw JK. Benzonitrile assisted enolization of the acetone and acetamide radical cations: proton-transport catalysis versus an intermolecular H+/D+ transfer mechanism. Int J Mass Spectrom. 2001;210/211:489–502.
27. Wang X, Holmes JL. A study of the isomerization and dissociation of formal [acetone-methanol]+. ion-molecule complexes. Can J Chem. 2005;83:1903–1912. and references therein.
28. Wang X, Holmes JL. Exploring the potential energy surface of ion-molecule pairs by experiment and by theory: acetaldehyde and methanol. Int J Mass Spectrom. 2005;242:75–85.
29. Sutton R. Esters and flavenes from 2-hydroxychalcones and flavylium salts. J Org Chem. 1972;37(7):1069–70.
30. Hendley EC, Neville OK. Carbon14 tracer studies in the rearrangements of unsymmetrical α-diketones. III. p-Methoxybenzylideneacetophenone oxide. J Am Chem Soc. 1953;75:1995–6.
31. Becker HD, Bremholt T. Tetrahedron Letters. 1973;14(3):197–200.
32. Gross ML. Tandem mass spectrometry: Multisector magnetic instruments. In: McCloskey JA, editor. Methods in Enzymology, Vol. 193, Mass Spectrometry. Academic Press; San Diego: 1990. pp. 131–153.
33. Stewart JJP, Frank JS. Optimization of parameters for semiempirical methods. I. Method. J Comp Chem. 1989;10:209–20.
34. Stewart JJP, Frank JS. Optimization of parameters for semiempirical methods. II. Applications. J Comp Chem. 1989;10:221–64.
35. Wittbrodt JM, Schlegel HB. Some reasons not to use spin projected density functional theory. J Chem Phys. 1996;105:6574–77.
36. Baker J, Scheiner A, Andzelm J. Spin contamination in density functional theory. J Chem Phys Lett. 1993;216:380–8.
37. Laming GJ, Hardy NC, Amos RD. Kohn-Sham calculation on open-shell diatomic molecules. Mol Phys. 1993;80:1121–34.
38. Nicolaides A, Smith DM, Jensen F, Radom LJ. Phenyl Radical, Cation, and Anion. The Triplet-Singlet Gap and Higher Excited States of the Phenyl Cation. J Am Chem Soc. 1997;119:8083–88.
39. Frisch MJ, Trucks GW, Schlegel HB, Scuseria GE, Robb MA, Cheeseman JR, Zakrzewski VG, Montgomery JA, Jr, Stratmann RE, Burant JC, Dapprich S, Millam JM, Daniels AD, Kudin KN, Strain MC, Farkas O, Tomasi J, Barone V, Cossi M, Cammi R, Mennucci B, Pomelli C, Adamo C, Clifford S, Ochterski J, Petersson GA, Ayala PY, Cui Q, Morokuma K, Malick DK, Rabuck AD, Raghavachari K, Foresman JB, Cioslowski J, Ortiz JV, Stefanov BB, Liu G, Liashenko A, Piskorz P, Komaromi I, Gomperts R, Martin RL, Fox DJ, Keith T, Al-Laham MA, Peng CY, Nanayakkara A, Gonzalez C, Challacombe M, Gill PMW, Johnson B, Chen W, Wong MW, Andres JL, Gonzalez C, Head-Gordon M, Replogle ES, Pople JA. Gaussian 98. Gaussian, Inc.; Pittsburgh PA: 1998. Revision A.6.
40. Frisch MJ, Frisch A. Gaussian 98, User’s Reference. Gaussian, Inc.; Pittsburgh, PA: 1999. , and references therein.
41. Scott AP, Radom L. Harmonic Vibrational Frequencies: An Evaluation of Hartree-Fock, Møller-Plesset, Quadratic Configuration Interaction, Density Functional Theory, and Semiempirical Scale Factors. J Phys Chem. 1996;100:16502–13.
42. Bongiorno1 D, Ceraulo1 L, Lamartina1 L, Natoli MC. Studies in organic mass spectrometry. Part 25. Benzyl ion formation in chemical ionization (methane or isobutane) of some orthoalkylhetero-substituted diphenylcarbinols. Rapid Commun Mass Spectrom. 2000;14:203–206. [PubMed]
43. Agozzino P, Ceraulo L, Ferrugia M, Lamartina L. A new general fragmentation reaction in mass spectrometry. The hydrogen-carbon, carbon-carbon double rearrangement of 2-heteroalkyl substituted diphenylmethyl cations. Eur Mass Spectrom. 1995;1:73–79.