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Neuroscience. Author manuscript; available in PMC 2010 July 7.
Published in final edited form as:
PMCID: PMC2689788
NIHMSID: NIHMS109140

Glial-Neuronal Interactions Underlying Fructose Utilization in Rat Hippocampal Slices

Abstract

Although fructose is commonly used as a sweetener, its effects on brain function are unclear. Using rat hippocampal slices, we found that fructose and mannose, like pyruvate, preserve ATP levels during 3-hours of glucose deprivation. Similarly, fructose and mannose restored synaptic potentials (EPSPs) depressed during glucose deprivation. However, restoration of synaptic responses was slow and only partial with fructose. EPSPs supported by mannose were inhibited by cytochalasin B (CCB), a glucose transport inhibitor, but were not inhibited by α-cyano-4-hydroxycinnamate (4-CIN), a monocarboxylate transport inhibitor, indicating that neurons use mannose via glucose transporters. In contrast, both CCB and 4-CIN depressed EPSPs supported by fructose, suggesting that fructose may be taken up by non-neuronal cells through CCB sensitive hexose transporters and metabolized to a monocarboxylate for subsequent use during neuronal respiration. Supporting this possibility, twenty minutes of oxygen deprivation in the presence of fructose resulted in functional and morphological deterioration whereas oxygen deprivation in the presence of glucose or mannose had minimal toxic effects. These results indicate that neuronal fructose utilization differs from glucose and mannose and likely involves release of monocarboxylates from glia.

Keywords: ATP, GLUT8, GLUT11, glycolysis, hexose, monocarboxylate

INTRODUCTION

Although glucose is the principal energy substrate in the mammalian central nervous system (CNS), there is evidence that other endogenous agents, including monocarboxylates and creatine (Nakashima et al., 2005), serve as energy substrates under certain conditions. In hippocampal slices, lactate (Schurr et al., 1988) and pyruvate (Izumi et al., 1994; 1997b) are examples of monocarboxylates that can sustain neuronal integrity in the absence of glucose.

Alternative energy substrates for glucose may not be limited to monocarboxylates, and hexoses other than glucose may also serve as CNS energy substrates. In the United States, it is estimated that per capita fructose consumption as corn syrup has increased to more than 40 g/day (Gaby, 2005). This dietary consumption raises questions about whether fructose can alter CNS energy metabolism (Funari et al., 2007). Prior studies indicate that fructose has memory-enhancing properties (Messier and White, 1987; Rodriguez et al., 1994) and can act as a neuroprotectant under some circumstances (Sapolsky, 1986). Moreover, high fructose intake may alter hypothalamic appetitive systems (Lindqvist et al., 2008).

These findings suggest that fructose has direct actions in the CNS, although whether fructose can be used as a brain energy substrate remains uncertain (Douard & Ferraris, 2008). Okada’s group initially reported that mannose and fructose partially preserve synaptic function in the absence of glucose in the guinea pig dentate gyrus (Saitoh et al., 1994). However, subsequent studies by the same group found that mannose and fructose fail to preserve synaptic transmission in the dentate gyrus and CA3 region, despite the fact that they sustain ATP levels (Kanatani et al, 1995; Wada et al., 1998). These studies raise important questions about whether the CNS has the ability to use fructose as an energy substrate.

In the present study, we examined whether fructose serves as an energy substrate in the CA1 region of rat hippocampal slices using cytochalasin B (CCB) to inhibit hexose transporters and α-cyano-4-hydroxycinnamate (4-CIN) to inhibit monocarboxylate transporters. In hippocampal slices, synaptic responses depress gradually following glucose removal or during administration of CCB. EPSPs sustained by glucose are not altered by 4-CIN but EPSPs sustained by monocarboxylates are rapidly depressed by 4-CIN, indicating that 4-CIN acts as an inhibitor of monocarboxylate transporters (Izumi et al., 1997a). The rapid decline of EPSPs following glucose deprivation in the presence of 4-CIN appears to result from block of monocarboxylate exchange between glia and neurons, suggesting that monocarboxylates released from glia fuel neurons when glucose use is limited (see also Allen et al., 2005; Sakurai et al., 2002; Cater et al., 2001). Because we previously observed that CCB suppresses glucose-supported EPSPs without affecting pyruvate-supported EPSPs at 50 μM, whereas 4-CIN suppresses pyruvate-mediated EPSPs without affecting glucose-supported EPSPs at 200 μM (Izumi et al., 1997a), we used 50 μM CCB and 200 μM 4-CIN to determine whether they affect mannose- and fructose-supported EPSPs. Using CCB and 4-CIN, we show that fructose supports neuronal function through release of monocarboxylates, likely produced in glia, rather than through a direct mechanism.

EXPERIMENTAL PROCEDURES

Hippocampal Slice Preparation

Slices were prepared from the septal half of the hippocampus using standard techniques. Postnatal day (PND) 30–34 albino rats were anesthetized with halothane and decapitated (Zorumski et al., 1996). Hippocampi were rapidly dissected and placed in artificial cerebrospinal fluid (ACSF) containing (in mM): 124 NaCl, 5 KCl, 2 MgSO4, 2 CaCl2, 1.25 NaH2PO4, 22 NaHCO3, 10 glucose, bubbled with 95% O2-5% CO2 at 4–6 °C, and sliced transversely into 500 μm slices using a WPI vibroslicer. Slices were then placed in an incubation chamber containing gassed ACSF for 2 hr at 30°C. At the time of study, slices were transferred one at a time to a submersion-recording chamber, and experiments were done at 30°C. Fructose, mannose and pyruvate were used with adjusted pH and a reduction in NaCl to maintain osmolality.

Synaptic Physiology

Extracellular recordings were obtained from the apical dendritic region for analysis of population EPSPs or the pyramidal cell body layer of CA1 for study of population spikes using 2 M NaCl glass electrodes with resistances of 5–10 M. Data were recorded and analyzed using an IBM-based computer system. The amplitude of population spikes and slopes of extracellularly recorded EPSPs were measured off-line using computer programs written in Axobasic (MDS Inc. Toronto, Canada). Analysis of EPSPs was confined to stimuli that evoked only small population spikes that did not distort the waveform. Evoked synaptic responses were elicited with 0.2 msec constant current pulses through a bipolar electrode (Rhodes Medical Instruments, Woodland Hill, CA) placed in the Schaffer collateral-commissural pathway. Synaptic responses in CA1 were monitored by applying single stimuli to the Schaffer collateral pathway every 60 sec at an intensity sufficient to elicit a 50%–60% maximal EPSP slope. This stimulation frequency yields stable synaptic responses and avoids alterations in synaptic function that can occur with higher frequencies of test pulses.

ATP assay

ATP levels were determined by luminometry (Zylux, Maryville TN, USA) using an ATP assay kit (Calbiochem-Novabiochem, San Diego, CA, USA). The assay is based on the firefly luciferase-catalyzed oxidation of D-luciferin in the presence of ATP and oxygen, whereby the amount of ATP is quantified by the amount of light produced with a luminometer (Femtomaster FB12, Zylux Corp., Pfortzheim, Germany). Protein concentrations were determined by standard procedure (BioRad, Hercules CA, USA) (Bradford et al.,1976). In control hippocampal slices incubated with 10 mM glucose, ATP levels were 20.4 ± 2.6 nmole/mg protein (N=14). ATP concentrations from each whole slice were compared to matched controls from the same hippocampus incubated and measured simultaneously during each experiment.

Statistics

Differences between groups were analyzed using Student’s t-test and paired-pulse Student’s t-test (SigmaStat, Jandel Scientific Software, San Rafael, CA).

Materials

All chemicals, except for the ATP assay kit, were purchased from Sigma Chemical Co. (St. Louis, MO). Rats were purchased from Harlan Sprague Dawley Inc. (Indianapolis, IN).

RESULTS

Effects of Energy Substrates on ATP levels during Glucose Deprivation

Prior studies indicate that fructose and mannose preserve ATP levels in hippocampal slices (Saitoh et al., 1994, Kanatani et al, 1995, Wada et al., 1998). To confirm these observations, we initially compared the ability of fructose, mannose and pyruvate to preserve ATP levels in the absence of glucose. In hippocampal slices from PND 30 rats, ATP levels are depressed by > 60% during a three-hour period of glucose deprivation (Figure 1). This depression of ATP levels was largely prevented by administration of 10 mM pyruvate during the period of glucose deprivation (< 20% depression). Similarly, the reduction in ATP levels was effectively prevented by administration of either 10 mM mannose or 10 mM fructose (Figure 1), suggesting that these two hexoses can serve as energy sources during glucose deprivation in the hippocampus.

Figure 1
Effects of alternative energy substrates on ATP levels during glucose deprivation. ATP levels were measured relative to those observed following incubation of hippocampal slices with 10 mM glucose for three hours. Three-hour incubation of hippocampal ...

Effects of Energy Substrates on Synaptic Transmission during Glucose Deprivation

To determine whether the beneficial effects of mannose and fructose on hippocampal ATP levels result in improved synaptic function, we examined the effects of these energy substrates on CA1 transmission during glucose deprivation. In hippocampal slices, CA1 EPSPs were suppressed gradually during sixty minutes of glucose deprivation. In previous work, we found that release of endogenous monocarboxylates, likely from glia, helps to sustain synaptic responses following glucose removal and that inhibitors of monocarboxylate transport greatly speed the depression (Izumi et al., 1997a).

Following complete synaptic depression, EPSPs were restored almost completely within 20 min by administration of 10 mM mannose (EPSPs; 86 ± 2% of control baseline at 20 min, n=5, Figure 2). EPSPs sustained by 10 mM mannose were again gradually depressed by application of 50 μM CCB, a hexose transport inhibitor, with complete elimination of responses taking more than 40 min. The depressed EPSPs were restored by subsequent administration of 10 mM pyruvate in the presence of CCB (61 ± 20% 20 min after pyruvate administration). The restoration of EPSPs by pyruvate in the presence of CCB was blocked if 4-CIN was administered 10 min before pyruvate administration (EPSPs; 4 ± 2%, n=4, data not shown). In another set of experiments, EPSPs sustained by 10 mM mannose were not depressed by 200 μM 4-CIN, a monocarboxylate transport inhibitor (100 ± 8%, n=5, Figure 3), suggesting that the preservation of neuronal activity by mannose does not result from the uptake of mannose via 4-CIN-sensitive transporters or the release of endogenous monocarboxylates from glia.

Figure 2
Effects of CCB on mannose-sustained EPSPs. Slices were initially incubated in ACSF containing 10 mM glucose (double hatched bar). Perfusion of glucose-free ACSF starting at time 0 gradually depressed EPSPs. After complete suppression of EPSPs, administration ...
Figure 3
Effects of 4-CIN on mannose-sustained EPSPs. EPSPs supported by 10 mM glucose (double hatched bar) were gradually depressed by perfusion of ACSF without glucose. Administration of 10 mM mannose (solid bar) restored EPSPs. The mannose-supported EPSPs were ...

Similar to the effects of mannose, administration of 10 mM fructose also restored EPSPs during glucose deprivation. Unlike mannose, however, the recovery of EPSPs in the presence of fructose was slow and only partial (EPSP; 20 ± 9% 20 min after fructose perfusion, n=5, P<0.01, with gradual recovery to about 80% of control, Figure 4). EPSPs sustained by 10 mM fructose were diminished by CCB, suggesting that fructose requires a CCB-sensitive hexose transporter (EPSP; 22 ± 7% 20 min after CCB administration, Figure 4). However, the decay was faster than that observed with mannose (P<0.05 vs. mannose), suggesting a distinct mechanisms for fructose use or additional effects of CCB on fructose use.

Figure 4
Effects of CCB on fructose-sustained EPSPs. Slices were initially incubated in ACSF containing 10 mM glucose (double hatched bar). Perfusion of glucose-free ACSF starting time 0 gradually depressed EPSPs. After complete suppression of EPSPs, administration ...

Because CCB, like other cytochalasins, also binds actin, we tested if fructose-sustained EPSPs are affected by cytochalasin D (CCD), an agent that more potently binds to actin but does not inhibit hexose tranporters (Cooper 1987). Because CCD depressed glucose-sustained EPSPs at 5 μM suggesting non-specific effects (Kim and Lisman, 1999, Chen, et al, 2004), we administered 1μM CCD, a concentration that binds actin but has no effect on glucose-supported synaptic responses. EPSPs restored by 10 mM fructose after glucose deprivation were not depressed by CCD (Figure 5), suggesting that effects on actin are unlikely to contribute to CCB-mediated inhibition of EPSPs sustained by fructose.

Figure 5
Effects of CCD on fructose-sustained EPSPs. Slices were initially incubated in ACSF containing 10 mM glucose (double hatched bar). Perfusion of glucose-free ACSF starting at time 0 gradually depressed EPSPs. After complete suppression of EPSPs, administration ...

In another set of experiments, EPSPs sustained by fructose were also rapidly diminished by 4-CIN (EPSP; 5 ± 2% 20 min after 4-CIN administration, n=5, P<0.01 vs. mannose), and, in the presence of 4-CIN, the depressed EPSPs were restored by administration of 10 mM glucose (Figure 6, EPSP; 74 ± 2%). These observations suggest that fructose is either transported by both CCB- and 4-CIN-sensitive mechanisms or is indirectly utilized by neurons. The latter possibility further suggests that non-neuronal cells (glia) may be involved in the effects of fructose (Magistretti and Pellerin, 1996), nourishing neurons through the delivery of monocarboxylates such as pyruvate.

Figure 6
Effects of 4-CIN on fructose-sustained EPSPs. EPSPs sustained by 10 mM glucose (double hatched bar) were gradually depressed by perfusion of ACSF without glucose. Administration of 10 mM fructose (solid bar) restored EPSPs. In contrast to mannose-supported ...

It has been recently reported that gap junctions and glio-neuronal interactions play a role in neuronal monocarboxylate use in the hippocampus (Rouach et al., 2008). Because we suspect that neuronal use of fructose is indirect through monocarboxylate transporters, we examined whether meclofenamic acid (MFA), a water-soluble gap junction inhibitor (Pan et al., 2007) alters fructose-sustained EPSPs. Fructose-sustained EPSPs after glucose deprivation were promptly depressed by 10μM MFA. Furthermore, EPSPs depressed in the presence of MFA were restored by administration of glucose (Figure 7, n=5), again supporting a distinct mechanism for fructose use.

Figure 7
Effects of MFA on fructose-sustained EPSPs. EPSPs sustained by 10 mM glucose (double hatched bar) were depressed by perfusion of ACSF without glucose. Administration of 10 mM fructose (solid bar) restored EPSPs. The fructose-sustained EPSPs were depressed ...

Effects of CCB and 4-CIN on ATP Levels Supported by Fructose

In slices from 5 animals, ATP levels were compared during 3 hour glucose deprivation with 10 mM fructose plus CCB or 4-CIN. Preservation of ATP levels by fructose is impaired by both CCB and 4-CIN, indicating that fructose is utilized through two distinct transporters (Fig. 8). In other experiments, 4-CIN did not depress ATP levels in the presence of 10 mM glucose (89 ± 11%, n=3). Figure 8 near here

Figure 8
Effects of CCB and 4-CIN on ATP levels supported by fructose. ATP levels were measured relative to those observed following incubation of hippocampal slices with 10 mM glucose for three hours. Three-hour incubation of hippocampal slices in ACSF in the ...

Effects of Energy Substrates on Hypoxia and Simulated Ischemia

If fructose helps to sustain neuronal function indirectly via metabolism in glia and subsequent release of endogenous monocarboxylates, then the use of fructose as an energy source should be restricted to conditions in which respiration remains intact because monocarboxylates require respiration for metabolic processing. To test this, we examined the effects of the energy substrates during hypoxia. Consistent with the requirement for respiration in the metabolic effects of monocarboxylates, EPSPs supported by 10 mM pyruvate were diminished by 20 min oxygen deprivation and did not recover following re-oxygenation (Figure 9, open triangles, n=4). In contrast, EPSPs supported by 10 mM glucose diminished during 20 min oxygen deprivation, but were completely restored by re-oxygenation in the presence of glucose (Izumi et al., 1998b; open circles, n=5). Similarly, in the presence of 10 mM mannose instead of glucose, suppression of EPSPs during oxygen deprivation was reversed by re-oxygenation (filled triangles, n=5). EPSPs supported by 10 mM fructose, however, were not restored during re-oxygenation (filled circles, n=5).

Figure 9
Effects of oxygen deprivation on EPSPs and ATP levels supported by various energy substrates. To induce hypoxia, oxygen (95% in normal ACSF) was replaced by nitrogen for 20 min (filled bar). A. EPSPs were complete suppressed during oxygen deprivation, ...

Although EPSPs were completely suppressed during oxygen deprivation regardless of the energy source, ATP levels after oxygen deprivation were surprisingly well preserved when slices were incubated with 10 mM glucose or 10 mM mannose (second and third bars in Figure 10, n=5). Twenty minutes of oxygen deprivation in the absence of any exogenous energy substrates (simulated ischemia) depressed ATP levels significantly (top bar in Figure 10). The same extent of depression was observed following anoxia in the presence of fructose (bottom bar in Figure 10). In another set of studies, anoxia in the presence of 10 mM pyruvate also significantly depressed ATP levels (22+5% of control, n=4). Figure 10 near here

Figure 10
ATP levels in whole slices twenty minutes after oxygen deprivation. Oxygen deprivation depressed ATP levels in the absence of any exogenous energy substrate by > 60% (first bar, P < 0.01 v.s. control levels with glucose and oxygen). In ...

DISCUSSION

There is considerable evidence that glucose is not the sole energy substrate used in the CNS. In hippocampal slices, the monocarboxylates, lactate (Schurr et al., 1988), pyruvate (Izumi et al., 1994) and β-hydroxybutyrate (Izumi et al., 1998a), sustain neuronal function during periods of glucose deprivation. In the present study, we provide evidence that hexoses other than glucose, specifically mannose and fructose, can support neuronal integrity in the hippocampus. Although plasma concentrations of mannose and fructose in healthy individuals are very low compared to glucose (Pitkänen, 1996), the concentrations of these hexoses in CSF are relatively higher (Kusmierz et al., 1989). While the physiological relevance of mannose as an energy substrate in the CNS is unclear, determining the possible roles of fructose in CNS energy metabolism is important because human fructose consumption is significant (Gaby, 2005) and it has been shown that fructose load affects brain function (Messier et al., 2007).

4-CIN is a useful tool for blocking monocarboxylate transporters selectively in hippocampal neurons, though at higher concentrations, 4-CIN also inhibits mitochondrial pyruvate uptake and oxidation in neurons and non-neuronal cells (Halestrap and Armston, 1984, McKenna et al., 2001,). Impairment of mitochondrial metabolism may suppress synaptic transmission (Tozzi et al., 2007). We previously observed that a high concentration of 4-CIN (2 mM) depresses glucose-supported EPSPs (Izumi et al., 1997a). Glucose-supported EPSPs are, however, not altered by 200 μM 4-CIN, suggesting that at 200 μM 4-CIN is more likely acting via effects on monocarboxylate transport than through inhibition of mitochondrial metabolism.

Similarly, CCB is a useful tool for blocking hexose transporters in hippocampal neurons. However, CCB, like other cytochalasins, also binds to actin. Among the various cytochalasins, CCD is the most potent in binding to actin, but does not inhibit hexose transporters (Cooper, 1987). Because it has been shown that CCD binds to actin and exhibits neuronal actions at 1 μM (Krucker et al., 2000, Wei et al., 2004) but at higher concentrations irreversibly inhibits glucose-sustained EPSPs and induces neurotoxicity (Kim and Lisman, 1999, Kim et al., 2002, Chen et al., 2004), we used 1 μM CCD for our studies. At 1 μM, CCD had no effect on fructose-sustained EPSPs. This observation suggests that actin-binding is unlikely to account for the depression of fructose-sustained EPSPs by CCB.

In the present study, we observed that 4-CIN does not block EPSPs supported by mannose, indicating that mannose is likely to be more directly utilized by neurons (Figure 11A). In contrast to mannose, fructose is transported by both 4-CIN and CCB-sensitive mechanisms. We hypothesize that fructose is first taken up by non-neuronal cells and then metabolized to a monocarboxylate for subsequent neuronal utilization (Figure 11B). Such a complex mechanism potentially underlying fructose utilization may be responsible for the only partial or less consistent ability of fructose to sustain EPSPs. In the CA3 region of guinea pig hippocampal slices, Okada’s group initially reported that EPSPs were sustained after switching energy substrates from glucose to fructose (Saitoh et al., 1994). However, the same group subsequently found that fructose failed to sustain EPSPs in spite of preserving ATP levels (Kanatani et al., 1995, Wada et al., 1998). Our results suggest that these inconsistencies may result from the apparent complex metabolic processing of fructose and factors that influence the integrity of glioneuronal interactions in slice preparations (Figure 11B).

Figure 11
Hypothetical mechanisms for use of mannose and fructose by neurons. A. A scheme for the use of mannose by neurons is displayed. If mannose, like glucose, is directly used by neurons via a hexose transporter, CCB, but not 4CIN, inhibits its uptake. B. ...

Our results examining the effects of hexoses during oxygen deprivation argue against fructose being used directly by neurons as an energy substrate. Brief oxygen deprivation does not result in severe neuronal damage as long as hexoses are available for use by neurons, while deprivation of both oxygen and glucose results in severe neuronal damage even if pyruvate is present. This difference indicates that during anoxia glycolysis is important for preserving neuronal integrity.

Whereas glial glycolysis may be critical for glutamate metabolism and preventing excitotoxicity (Poitry et al., 2000), neuronal glycolysis may be crucial for maintaining synaptic function. This is because neurons lack glycogen and can preserve glycolytic energy metabolism only when hexoses are directly available. The failure of EPSPs to recover after oxygen deprivation in the presence of fructose indicates that fructose cannot be metabolized by neurons during oxygen deprivation. Even though it appears that fructose can be used by glia during anoxia, anoxia in the presence of fructose results in poor recovery of neuronal function. This suggests that neuronal glycolytic metabolism may be critical for preserving function during anoxia.

GLUT5, a specific fructose transporter, is expressed in the CNS (Shepherd et al., 1992; Maher et al., 1996; Payne et al., 1997) and insults that decrease the activity of the glucose transporters, GLUT1 and GLUT3, increase GLUT5 activity (Payne et al., 1997; Vannucci et al., 2003). This suggests that fructose transported through GLUT5 may be of particular importance under conditions where neuronal glucose uptake is impaired. However, GLUT5 is expressed only in microglia (Horikoshi, 2003). Furthermore, it has recently been reported that fructose loading improves memory tasks but does not increase GLUT5 expression in the brain (Messier et al., 2007), suggesting that the positive effects of fructose loading on cognitive function are independent of GLUT5 up-regulation. In the present study, the complete suppression of fructose-sustained EPSPs by CCB indicates that a hexose transporter sensitive to CCB is responsible for fructose utilization in the hippocampus. Additionally, CCB does not appear to inhibit GLUT5-mediated hexose uptake (Burant et al., 1992; Garriga et al., 2004). Taken together, these observations raise the possibility that fructose utilization in the CNS is mediated by a hexose transpoter distinct from GLUT5 and that other glucose transporters in CNS are involved in fructose uptake. Fructose is also transported by other Class II GLUTs such as GLUT9 and GLUT11 (Scheepers et al., 2005, Manolescu et al., 2007), both of which are expressed in brain (Doege et al., 2001; Wu et al., 2002; Augustin et al., 2004). In addition, GLUT8, also called X1, transports fructose (Ibberson et al., 2000) and is expressed in brain (Ibberson et al., 2002, Reagan et al., 2002, Widmer et al., 2005). Among these glucose transporters that promote fructose uptake, GLUT8 and GLUT11 are known to bind CCB (Ibberson et al. 2000, Wu et al., 2002), whereas GLUT9 is not sensitive to CCB (Doege et al., 2000, Augustin et al., 2004). Thus, GLUT8 and GLUT11 or an as yet unknown transporter may be involved in fructose uptake in the CNS. It should be also noted that these transporters may have high affinity for fructose which would allow them to scavenge the low concentrations of fructose that are likely found in the CSF.

In summary, our findings suggest that fructose is not directly utilized by neurons in the CA1 region of hippocampus. Different from glucose and mannose, fructose use appears to be complex with uptake and metabolism through a monocarboxylate transporter that is likely localized to non-neuronal cells. Although fructose utilization by neurons is indirect, fructose may still be a useful energy substrate for preserving neuronal activity when glucose transport is compromised, though we did not directly examine neuronal effects of lower concentrations of fructose that are more likely found in the CNS. Thus, further investigation is needed to determine the physiological role of fructose in the CNS.

Acknowledgments

This work was supported in part by National Institute of Health grants MH077791, AG18434 and AA12951, Alzheimer’s Disease Research Center of Washington University and the Bantly Foundation.

Abbreviations

ATP
adenosine triphosphate
EPSPs
excitatory postsynaptic potentials
CCB
cytochalasin B
4-CIN
α-cyano-4-hydroxycinnamate
CNS
central nervous system
PND
Postnatal day
ACSF
artificial cerebrospinal fluid

Footnotes

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