Search tips
Search criteria 


Mol Biochem Parasitol. 2007 July; 154(1-4): 110–114.
PMCID: PMC2686105

Role of the stage-regulated nucleoside transporter TbNT10 in differentiation and adenosine uptake in Trypanosoma brucei

Trypanosoma brucei undergoes complex metabolic and morphological changes during its life cycle in order to adapt and survive inside the mammalian host and the tsetse fly vector. One of these adaptations to different environments is reflected by the expression of various nucleoside transporters. As protozoan parasites are unable to synthesise the purine ring de novo, they have to salvage preformed nucleosides and nucleobases from their hosts [1]. To date, several nucleoside transporters of T. brucei have been identified and characterised, all of which belong to the equilibrative nucleoside transporter family. Depending on their substrate specificities they can be assigned to two types of transport activities, P1 and P2 [2]. P1 activity is specific for the uptake of adenosine, guanosine and inosine and has been found in both bloodstream and procyclic forms. P2 activity is specific for adenosine and adenine uptake. Only one P2 transporter, TbAT1, has been described so far. TbAT1 is expressed in bloodstream forms and has been shown to transport the trypanocidal drugs melarsoprol and pentamidine [2,3]. Additionally, purines can be salvaged by the hypoxanthine transporting activities H1–H4 [4,5].

Although many nucleoside transporters and their substrate specificities have been characterised, the reason for the parasite to employ many related transporters with the seemingly redundant function of purine uptake, is still unclear. Furthermore, very little is known about the contribution of single transporters to purine uptake in different life cycle stages of the parasite.

The nucleoside transporter TbNT10 [6] (Tb09.160.5480, also known as TbAT-B [7]), displays a P1-type transport activity with high affinities for adenosine, guanosine and inosine and marginal affinities for hypoxanthine and adenine [6,7]. TbNT10 mRNA was shown to be stage-regulated in T. brucei rhodesiense EATRO 2340, with highest expression in short stumpy bloodstream forms and lowest expression in long slender bloodstream and procyclic forms [6]. This regulated expression during the life cycle might point to a function of the transporter in the process of differentiation.

In this study a possible role of TbNT10 in differentiation from long slender to short stumpy bloodstream forms, and in the following differentiation step to procyclic forms, was investigated. Northern blot analysis with mRNA from different life cycle stages of T. brucei brucei strain AnTat 1.1 [8,9] confirmed that the TbNT10 mRNA is expressed at a low level in long slender bloodstream forms and is up-regulated in short stumpy bloodstream forms (Fig. 1A). In this strain, however, the highest level of TbNT10 mRNA was found in procyclic forms. Strain-specific differences have been observed previously for the expression of another P1-type transporter TbNT2 [10]. In addition, culture conditions might influence expression levels of certain transporters. The TbNT10 mRNA is 4.2 kb long, with an open reading frame (ORF) of 1.4 kb. In order to determine the length of the untranslated regions (UTRs) a cDNA library [11] was screened and cDNAs containing spliced leader sequences were analysed. The 5′ UTR was found to be 42 bases. We could not determine the precise length of the 3′ UTR, as this region contains several AU-rich stretches that supported internal priming. Considering the Northern blot data, however, we estimate the 3′ UTR to be ~2.7 kb long.

Fig. 1
TbNT10 mRNA expression and role in differentiation. (A) Northern blot analysis with 10 μg total RNA from long slender (LS) and short stumpy (SS) bloodstream forms, and early (P+) and late (P−) procyclic forms of AnTat 1.1 wild ...

As mentioned above, the regulated expression of TbNT10 mRNA could point to several functions in the differentiation from long slender to short stumpy bloodstream forms. Short stumpy forms are pre-adapted to be taken up by the tsetse fly. They are unable to divide in the mammalian bloodstream and die within a few days. The differentiation from long slender into short stumpy bloodstream forms is triggered by a stumpy inducing factor (SIF) that accumulates in a cell-density dependent manner in culture medium (and presumably blood) containing long slender parasites [12]. SIF has been characterised as a small compound with a molecular weight of ≤500 Da. Its chemical identity is still unclear, but it could potentially be a nucleoside since membrane permeable cAMP analogues mimic SIF activity [12].

In order to investigate the role of TbNT10 in the response to SIF, a null mutant (Δnt10::NEO/Δnt10::HYG) was constructed in T. brucei AnTat 1.1 (Fig. 1A). For technical reasons, this was done in procyclic forms, as these are considerably easier to transfect. Southern blot analysis confirmed the correct integration of the two antibiotic resistance cassettes into the TbNT10 loci (data not shown) and Northern blot analysis confirmed the absence of TbNT10 mRNA in the null mutant (Fig. 1A). Early procyclic forms of the null mutant showed no phenotype in growth or in differentiation from early to late procyclic forms, as assessed by the loss of GPEET procyclin [13] (data not shown). Furthermore, the null mutant was still able to infect tsetse flies (Glossina morsitans morsitans), and subsequently mice, from which long slender bloodstream forms were isolated for further analysis. In order to test whether TbNT10 is required for differentiation from the long slender to short stumpy bloodstream form, cultures of the wild type and the TbNT10 null mutant were compared (Fig. 1B). Differentiation to the stumpy form was monitored by the diaphorase assay [14]. The null mutant entered stationary phase at a lower cell density, but differentiated to the short stumpy bloodstream form as fast as the wild type. To determine if SIF might be taken up by other P1 transporters, wild-type cells were cultured in the presence of 1 mM inosine, which is a P1 substrate and, hypothetically, could compete with SIF (Fig. 1B). However, wild-type cells exposed to inosine were able to differentiate from long slender to short stumpy bloodstream forms as well as cells without the competitor. These results indicate that neither TbNT10, nor any other P1 transporter, is responsible for the uptake of SIF in cell culture. In addition, a function of TbNT10 in the maintenance of short stumpy forms or the differentiation to procyclic forms could be excluded, as stumpy forms of the null mutant were still able to differentiate to procyclic forms and express EP procyclins with identical kinetics to the wild type (data not shown).

The situation in vivo was analysed by comparing mice infected with the TbNT10 null mutant and the wild type. Total parasitaemia, the proportion of dividing and non-dividing cells and differentiation from long slender to short stumpy bloodstream forms were compared between the two groups (Fig. 1C, D). The null mutant parasites established a peak parasitaemia similar to the wild type, but showed a two-fold lower cell density 6–10 days post infection. However, the ratio between dividing and non-dividing cells (Fig. 1C) and the kinetics of differentiation into growth-arrested short stumpy bloodstream forms (Fig. 1D) were the same as for wild-type cells. These data confirmed the findings in vitro that TbNT10 is not involved in the differentiation of long slender to short stumpy bloodstream forms. The lower parasite density during differentiation to short stumpy bloodstream forms in vivo as well as in vitro might point to a shortage of purines in the TbNT10 null mutant. Nevertheless, TbNT10 is not an essential gene, as the null mutant could be transmitted by tsetse flies and complete the entire life cycle.

An ectopically expressed GFP-TbNT10 fusion protein showed a surface localisation in procyclic forms (Fig. 2A). Taken together with the high expression of TbNT10 mRNA in early and late procyclic forms (Fig. 1A), this points to a likely function for the transporter in the insect stages of the parasite, with the up-regulation of mRNA in stumpy forms perhaps being a pre-adaptation for this. So far, two P1-type nucleoside transport activities TbNT2 and TbNT5 [10] have been described for procyclic forms and TbNT10 might also contribute to the uptake of purines and purine analogues. As a first step we measured the sensitivity to two toxic adenosine analogues, tubercidin (7-deazaadenosine) and cordycepin (3′-deoxyadenosine). The IC50 values of procyclic forms for tubercidin and cordycepin were found to be in the μM range, which is approximately 1000-fold higher than described for bloodstream forms [15]. In the drug sensitivity assays, procyclic forms of the null mutant were found to be 3.6 times more resistant to both tubercidin and cordycepin compared to wild-type parasites (Fig. 2C). In contrast, there was no difference between the wild type and TbNT10 null mutant in their sensitivity to the two trypanocidal drugs, that are taken up by the P2 transporter TbAT1 in bloodstream forms [2,16], melarsen oxide and pentamidine (data not shown). To confirm that the increased resistance to tubercidin and cordycepin was due to the depletion of TbNT10, two independent addback clones were constructed (Fig. 2B and C). The TbNT10 mRNA levels in both clones were comparable to that of the endogenous transcript, as shown by Northern blot (Fig. 2B). Ectopic expression of TbNT10 not only restored the sensitivity to tubercidin, but enhanced it up to 13-fold compared to wild-type cells. A similar trend was found in the response to cordycepin, as the two addback clones became approximately five-fold more sensitive than wild-type cells. In order to determine the contribution of TbNT10 to total P1-type purine uptake, two substrates, inosine and adenosine, were added to the drug assays as competitors (Fig. 2C). An excess of competitor (1 mM) increased drug resistance in all cell types, except the null mutant. The addition of competitors did not substantially increase the resistance of the wild type above that of the null mutant (without competitors); however, indicating that most P1-type transport was due to TbNT10.

Fig. 2
(A) Localisation of a GFP-TbNT10 fusion protein. The TbNT10 ORF was cloned into the BamHI site of the vector pG-EGFP-ΔLIIγ [18] downstream of the GFP ORF. The construct was transiently transfected into AnTat 1.1 procyclic forms and expression ...

Although the addbacks became more resistant to tubercidin in the presence of either competitor, they did not reach the same level of resistance as the wild type, possibly because the transporters were not saturated. Unexpectedly, however, they became highly resistant to cordycepin in the presence of adenosine. This could reflect the mode of action of cordycepin which, unlike tubercidin, is able to compete with adenosine intracellularly for integration into nucleic acids. Alternatively, this could be a consequence of different affinities of TbNT10 for the various substrates. Another unexpected finding was that the null mutant became more sensitive to cordycepin when competitors, particularly adenosine, were added. A similar outcome was recently reported for a TbAT1 null mutant in bloodstream forms that became hypersensitive to cordycepin in the presence of adenine [15]. One possibility is that crosstalk between different transporters might be involved in this effect, as other transporters could be up-regulated in the null mutant and might also contribute to drug uptake. Thus, although we cannot explain all effects observed in the drug assays, they clearly show a significant contribution by TbNT10 in the uptake of purine analogues by procyclic forms of T. brucei.

In order to quantify the contribution of TbNT10, the rates of adenosine uptake were compared in the wild type, the null mutant and one addback clone (Fig. 2D). Wild type cells showed strong adenosine uptake in the range of 50 pmol/107 cells after 5 min. Consistent with the results from the drug sensitivity assays, adenosine uptake by the addback mutant was increased to 70 pmol/107 cells, while uptake by the TbNT10 null mutant never exceeded 10 pmol/107 cells after 5 min (Fig. 2D). Once again, these results indicate a major role for TbNT10 in adenosine uptake by procyclic forms, although the parasites clearly have other means to acquire purines (for example, via hypoxathine transporters).

In conclusion, the stage regulated nucleoside transporter TbNT10 is not involved in the differentiation from long slender to short stumpy forms or in the transition to procyclic forms. It is not an essential gene, since null mutants can complete the whole life cycle. Nevertheless, we have demonstrated that in procyclic forms, where high levels of TbNT10 mRNA are expressed, purines and toxic analogues are imported by TbNT10 and that it is the main transporter for adenosine in this life cycle stage.


This research was funded by a Howard Hughes Medical Institute International Scholar's award and a grant from the Swiss National Science Foundation to IR and by a Wellcome Trust programme grant (073358) to KM.


1. Hammond D.J., Gutteridge W.E. Purine and pyrimidine metabolism in the Trypanosomatidae. Mol Biochem Parasitol. 1984;13:243–261. [PubMed]
2. Carter N.S., Fairlamb A.H. Arsenical-resistant trypanosomes lack an unusual adenosine transporter. Nature. 1993;361:173–176. [PubMed]
3. Mäser P., Sutterlin C., Kralli A., Kaminsky R. A nucleoside transporter from Trypanosoma brucei involved in drug resistance. Science. 1999;285:242–244. [PubMed]
4. Burchmore R.J., Wallace L.J., Candlish D. Cloning, heterologous expression, and in situ characterization of the first high affinity nucleobase transporter from a protozoan. J Biol Chem. 2003;278:23502–23507. [PubMed]
5. de Koning H.P., Jarvis S.M. Hypoxanthine uptake through a purine-selective nucleobase transporter in Trypanosoma brucei brucei procyclic cells is driven by protonmotive force. Eur J Biochem. 1997;247:1102–1110. [PubMed]
6. Sanchez M.A., Drutman S., van Ampting M., Matthews K., Landfear S.M. A novel purine nucleoside transporter whose expression is up-regulated in the short stumpy form of the Trypanosoma brucei life cycle. Mol Biochem Parasitol. 2004;136:265–272. [PubMed]
7. Al-Salabi M.I., Wallace L.J., Luescher A. Molecular interactions underlying the unusually high adenosine affinity of a novel Trypanosoma brucei nucleoside transporter. Mol Pharmacol. 2007;71(3):921–929. [PubMed]
8. Le Ray D., Barry J.D., Easton C., Vickerman K. First tsetse fly transmission of the “AnTat” serodeme of Trypanosoma brucei. Ann Soc Belg Med Trop. 1977;57:369–381. [PubMed]
9. Delauw M.F., Pays E., Steinert M., Aerts D., Van Meirvenne N., Le Ray D. Inactivation and reactivation of a variant-specific antigen gene in cyclically transmitted Trypanosoma brucei. EMBO J. 1985;4:989–993. [PubMed]
10. Sanchez M.A., Tryon R., Green J., Boor I., Landfear S.M. Six related nucleoside/nucleobase transporters from Trypanosoma brucei exhibit distinct biochemical functions. J Biol Chem. 2002;277:21499–21504. [PubMed]
11. Liniger M., Bodenmüller K., Pays E., Gallati S., Roditi I. Overlapping sense and antisense transcription units in Trypanosoma brucei. Mol Microbiol. 2001;40:869–878. [PubMed]
12. Vassella E., Reuner B., Yutzy B., Boshart M. Differentiation of African trypanosomes is controlled by a density sensing mechanism which signals cell cycle arrest via the cAMP pathway. J Cell Sci. 1997;110(Pt 21):2661–2671. [PubMed]
13. Vassella E., Den Abbeele J.V., Bütikofer P. A major surface glycoprotein of trypanosoma brucei is expressed transiently during development and can be regulated post-transcriptionally by glycerol or hypoxia. Genes Dev. 2000;14:615–626. [PubMed]
14. Vickerman K. Polymorphism and mitochondrial activity in sleeping sickness trypanosomes. Nature. 1965;208:762–766. [PubMed]
15. Geiser F., Lüscher A., de Koning H.P., Seebeck T., Mäser P. Molecular pharmacology of adenosine transport in Trypanosoma brucei: P1/P2 revisited. Mol Pharmacol. 2005;68(3):589–595. [PubMed]
16. de Koning H.P., Anderson L.F., Stewart M., Burchmore R.J., Wallace L.J., Barrett M.P. The trypanocide diminazene aceturate is accumulated predominantly through the TbAT1 purine transporter: additional insights on diamidine resistance in african trypanosomes. Antimicrob Agents Chemother. 2004;48:1515–1519. [PMC free article] [PubMed]
17. Flück C., Salomone J.Y., Kurath U., Roditi I. Cycloheximide-mediated accumulation of transcripts from a procyclin expression site depends on the intergenic region. Mol Biochem Parasitol. 2003;127:93–97. [PubMed]
18. Burkard G., Fragoso C.M., Roditi I. Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei. Mol Biochem Parasitol. 2007;153(2):220–223. [PubMed]
19. Domenicali Pfister D., Burkard G., Morand S., Kunz Renggli C., Roditi I., Vassella E. A mitogen-activated protein kinase controls differentiation of bloodstream forms of Trypanosoma brucei. Eukaryot Cell. 2006;5:1126–1135. [PMC free article] [PubMed]
20. de Koning H.P., Watson C.J., Jarvis S.M. Characterization of a nucleoside/proton symporter in procyclic Trypanosoma brucei brucei. J Biol Chem. 1998;273:9486–9494. [PubMed]