PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
 
J Clin Microbiol. 1985 November; 22(5): 828–834.
PMCID: PMC268536

Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types.

Abstract

The relationship of capsular types of Staphylococcus aureus to type of infection, carrier state, and phage type was studied in a collection of 477 isolates from 380 infection sites. Capsular polysaccharides were demonstrated by precipitation and agglutination with 11 monospecific antisera. When only one isolate from each infection was considered, 63% were of type 8 and 16% were of type 5. Of all the isolates tested, over 90% were encapsulated. We did not demonstrate any marked difference in the distribution of capsular types between isolates from the blood stream or purulent processes and isolates from healthy carriers or food. Most isolates from bovine mastitis milk had nontypeable capsules. The capsular type seemed stable in culture, and encapsulation had no apparent influence on susceptibility to phages. Of 27 phage-propagating strains maintained via culture transfer on artificial media over many years, 16 (59%) produced capsules. A striking association between certain phage patterns and capsular types was demonstrated.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aly R, Shinefield HR, Litz C, Maibach HI. Role of teichoic acid in the binding of Staphylococcus aureus to nasal epithelial cells. J Infect Dis. 1980 Apr;141(4):463–465. [PubMed]
  • Anderson JC. Absence of encapsulation in strains of Staphylococcus aureus isolated from bovine mastitis. Res Vet Sci. 1984 Nov;37(3):359–361. [PubMed]
  • Arbeit RD, Karakawa WW, Vann WF, Robbins JB. Predominance of two newly described capsular polysaccharide types among clinical isolates of Staphylococcus aureus. Diagn Microbiol Infect Dis. 1984 Apr;2(2):85–91. [PubMed]
  • Bernheimer HP. Lysogeny in pneumococci freshly isolated from man. Science. 1977 Jan 7;195(4273):66–68. [PubMed]
  • Bernheimer HP. Lysogenic pneumococci and their bacteriophages. J Bacteriol. 1979 May;138(2):618–624. [PMC free article] [PubMed]
  • Bernheimer HP, Tiraby JG. Inhibition of phage infection by pneumococcus capsule. Virology. 1976 Aug;73(1):308–309. [PubMed]
  • Fournier JM, Vann WF, Karakawa WW. Purification and characterization of Staphylococcus aureus type 8 capsular polysaccharide. Infect Immun. 1984 Jul;45(1):87–93. [PMC free article] [PubMed]
  • Guinan ME, Dan BB, Guidotti RJ, Reingold AL, Schmid GP, Bettoli EJ, Lossick JG, Shands KN, Kramer MA, Hargrett NT, et al. Vaginal colonization with Staphylococcus aureus in healthy women: a review of four studies. Ann Intern Med. 1982 Jun;96(6 Pt 2):944–947. [PubMed]
  • Hedström SA, Malmqvist T. Sphingomyelinase activity of Staphylococcus aureus strains from recurrent furunculosis and other infections. Acta Pathol Microbiol Immunol Scand B. 1982 Jun;90(3):217–220. [PubMed]
  • Ivler D. Comparative metabolism of virulent and avirulent staphylococci. Ann N Y Acad Sci. 1965 Jul 23;128(1):62–80. [PubMed]
  • Kaplan MH, Tenenbaum MJ. Staphylococcus aureus: cellular biology and clinical application. Am J Med. 1982 Feb;72(2):248–258. [PubMed]
  • Karakawa WW, Kane JA. Characterization of the surface antigens of Staphylococcus aureus, strain K-93M. J Immunol. 1972 May;108(5):1199–1208. [PubMed]
  • Melly MA, Duke LJ, Liau DF, Hash JH. Biological properties of the encapsulated Staphylococcus aureus M. Infect Immun. 1974 Aug;10(2):389–397. [PMC free article] [PubMed]
  • Opdebeeck JP, Norcross NL. Frequency and immunologic cross-reactivity of encapsulated Staphylococcus aureus in bovine milk in New York. Am J Vet Res. 1983 Jun;44(6):986–988. [PubMed]
  • Peterson PK, Wilkinson BJ, Kim Y, Schmeling D, Quie PG. Influence of encapsulation on staphylococcal opsonization and phagocytosis by human polymorphonuclear leukocytes. Infect Immun. 1978 Mar;19(3):943–949. [PMC free article] [PubMed]
  • Ritz HL, Kirkland JJ, Bond GG, Warner EK, Petty GP. Association of high levels of serum antibody to staphylococcal toxic shock antigen with nasal carriage of toxic shock antigen-producing strains of Staphylococcus aureus. Infect Immun. 1984 Mar;43(3):954–958. [PMC free article] [PubMed]
  • ROBBINS PW, UCHIDA T. Studies on the chemical basis of the phage conversion of O-antigens in the E-group Salmonellae. Biochemistry. 1962 Mar;1:323–335. [PubMed]
  • Sheagren JN. Staphylococcus aureus. The persistent pathogen (first of two parts). N Engl J Med. 1984 May 24;310(21):1368–1373. [PubMed]
  • Sompolinsky D, Schwartz D, Samra Z, Steinmetz J, Siegman-Igra Y. Septicemia with two distinct strains of Staphylococcus aureus and dwarf variants of both. Isr J Med Sci. 1985 May;21(5):434–440. [PubMed]
  • Wilkinson BJ, Holmes KM. Staphylococcus aureus cell surface: capsule as a barrier to bacteriophage adsorption. Infect Immun. 1979 Feb;23(2):549–552. [PMC free article] [PubMed]
  • Witte W. Kapselbildung bei Staphylococcus aureus als Ursache für die Nicht-Typisierbarkeit durch Phagen. Zentralbl Bakteriol Orig A. 1975 Dec;233(4):447–451. [PubMed]
  • Levin DL, Fixler DE, Morriss FC, Tyson J. Morphologic analysis of the pulmonary vascular bed in infants exposed in utero to prostaglandin synthetase inhibitors. J Pediatr. 1978 Mar;92(3):478–483. [PMC free article] [PubMed]
  • Yoshida K, Ohtomo T. Alteration of capsular type of encapsulated strains of Staphylococcus aureus during freeze-drying and storage. Experientia. 1984 Jan 15;40(1):93–94. [PubMed]
  • Yoshida K, Takahashi M, Ohtomo T, Minegishi Y, Ichiman Y, Haga K, Kono E, San Clemente CL. Application of fluorescent antibody for detecting capsular substances in Staphylococcus aureus. J Appl Bacteriol. 1979 Feb;46(1):147–152. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)