Search tips
Search criteria 


Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. 1985 October; 22(4): 641–644.
PMCID: PMC268483

Detection of herpes simplex virus type 2-specific antibody with glycoprotein G.


A recently described herpes simplex virus (HSV) type 2 (HSV-2)-specific glycoprotein (gG-2) was purified on an immunoaffinity column prepared with monoclonal antibody. This purified antigen was used in an immunodot enzymatic assay on nitrocellulose paper for the detection of HSV-2 antibodies in human serum. The test was very sensitive in that HSV-2 antibodies were detected in the convalescent sera of 132 of 134 patients with recurrent genital infections in which HSV-2 had been isolated earlier. Antibodies to gG-2 were detected in 17% of sera obtained within 10 days after the onset of a primary HSV infection and in 95% of sera obtained more than 10 days after onset. The specificity of the immunodot assay was demonstrated by testing sera from 245 HSV-seronegative adults, 344 children, 29 nuns, and 13 patients with primary genital HSV-1 infections. None of these 631 sera was reactive with the gG-2 antigen. When compared with a microneutralization test, the immunodot assay was found to be more specific in detecting HSV-2 antibodies. Reproducibility of the gG-2 assay, obtained by retesting 391 sera, was 95%. Thus, this assay has the sensitivity, specificity, and reproducibility necessary for the measurement of HSV-2 antibodies in seroepidemiological studies.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (699K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Arvin AM, Koropchak CM, Yeager AS, Pereira L. Detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein C purified with monoclonal antibody. Infect Immun. 1983 Apr;40(1):184–189. [PMC free article] [PubMed]
  • Buchman TG, Simpson T, Nosal C, Roizman B, Nahmias AJ. The structure of herpes simplex virus DNA and its application to molecular epidemiology. Ann N Y Acad Sci. 1980;354:279–290. [PubMed]
  • Coleman RM, Pereira L, Bailey PD, Dondero D, Wickliffe C, Nahmias AJ. Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Aug;18(2):287–291. [PMC free article] [PubMed]
  • Marsden HS, Stow ND, Preston VG, Timbury MC, Wilkie NM. Physical mapping of herpes simplex virus-induced polypeptides. J Virol. 1978 Nov;28(2):624–642. [PMC free article] [PubMed]
  • Nahmias AJ, Dowdle WR. Antigenic and biologic differences in herpesvirus hominis. Prog Med Virol. 1968;10:110–159. [PubMed]
  • Nahmias AJ, Dowdle WR, Kramer JH, Luce CF, Mansour SC. Antibodies to Herpesvirus hominis types 1 and 2 in the rabbit. J Immunol. 1969 Apr;102(4):956–962. [PubMed]
  • Nahmias AJ, Josey WE, Naib ZM, Luce CF, Duffey A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. I. Patients with genital herpetic infections. Am J Epidemiol. 1970 Jun;91(6):539–546. [PubMed]
  • Patterson WR, Rawls WE, Smith KO. Differentiation of serum antibodies to herpesvirus types 1 and 2 by radioimmunoassay. Proc Soc Exp Biol Med. 1978 Feb;157(2):273–277. [PubMed]
  • Pereira L, Dondero DV, Gallo D, Devlin V, Woodie JD. Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies. Infect Immun. 1982 Jan;35(1):363–367. [PMC free article] [PubMed]
  • Roizman B, Norrild B, Chan C, Pereira L. Identification and preliminary mapping with monoclonal antibodies of a herpes simplex virus 2 glycoprotein lacking a known type 1 counterpart. Virology. 1984 Feb;133(1):242–247. [PubMed]
  • SCHNEWEIS KE. [Serological studies on the type differentiation of Herpesvirus hominis]. Z Immun exp ther. 1962 Sep;124:24–48. [PubMed]
  • Schneweis KE, Nahmias AJ. Antigens of Herpes simplex virus type 1 and 2-immunodiffusion and inhibition passive hemagglutination studies. Z Immunitatsforsch Exp Klin Immunol. 1971 Jun;141(5):471–487. [PubMed]
  • Stavraky KM, Rawls WE, Chiavetta J, Donner AP, Wanklin JM. Sexual and socioeconomic factors affecting the risk of past infections with herpes simplex virus type 2. Am J Epidemiol. 1983 Jul;118(1):109–121. [PubMed]
  • Vonka V, Kanka J, Hirsch I, Závadová H, Krcmár M, Suchánková A, Rezácová D, Broucek J, Press M, Domorázková E, et al. Prospective study on the relationship between cervical neoplasia and herpes simplex type-2 virus. II. Herpes simplex type-2 antibody presence in sera taken at enrollment. Int J Cancer. 1984 Jan 15;33(1):61–66. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)