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Logo of diabetesSubscribeSearchDiabetes JournalAmerican Diabetes Association
 
From:
Published online 2009 February 19. doi: 10.2337/db08-1651

FIG. 2.

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Increased β-cell proliferation in high-fat diet young mice correlates with increased Bmi1 levels. A: Percentage of Ki67-positive β-cells of young and old mice after 8 weeks of normal diet or high-fat diet feeding. Values are averaged from three slides for each mouse and three mice for each group. ***P < 0.005. B: Percentage of PCNA-positive β-cells of young and old mice after 8 weeks of normal diet or high-fat diet feeding. Values are averaged from three slides for each mouse and three mice in each group. **P < 0.05 for young mice fed normal diet vs. young mice fed high-fat diet. C: Protein levels of p16 in isolated islets from 1-, 4-, 6-, and 11-month-old mice. Data were representative of pooled islets from 3–5 mice per group. D: Representative picture of pancreatic sections from 1- and 6-month-old wild-type mice stained with antibodies to p16 (red) and insulin (green). E: Levels of p16 and Bmi1 protein expression in the pooled islet cells isolated from 3–4 normal diet or high-fat diet young and old pancreata. Data were representative of pooled islets from 3–4 mice per group. Graph demonstrates densitometric analysis of immunoblot of p16 and Bmi1 normalized to β-tubulin. For all panels, data are representative of three experiments. **P < 0.05 for high-fat diet young mice vs. high-fat diet old mice. HFD, high-fat diet; ND, normal diet; Tub, β-tubulin. (A high-quality digital representation of this figure is available in the online issue).

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