Histone acetylations mark origins of polycistronic transcription in Leishmania major

doi:10.1186/1471-2164-10-152

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BMC Genomics. 2009; 10: 152.

Published online 2009 April 8. doi: 10.1186/1471-2164-10-152

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Figure 1

Antisera against TBP and SNAP₅₀preferentially precipitate tRNA/snRNA sequences. L. major chromatin was immunoprecipitated using antisera against TBP (top panel) or SNAP₅₀(middle panel) and PCR amplification carried out using primers specific for the U2/tRNA_Alalocus on chr31, which is bound by both TBP and SNAP₅₀in L. tarentolae [24] and the LmjF01.0530 gene on chr1 as a control. The numbers at top represent PCR cycles. The input control in the bottom panel represents amplification of chromatin without immunoprecipitation.

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