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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Phys Chem A. Author manuscript; available in PMC 2009 May 7.
Published in final edited form as:
PMCID: PMC2678804
NIHMSID: NIHMS49380

Photophysical Properties of Acene DCDHF Fluorophores: Long-Wavelength Single-Molecule Emitters Designed for Cellular Imaging

Abstract

We report the solvatochromic, viscosity-sensitive, and single-molecule photophysics of the fluorophores DCDHF-N-6 and DCDHF-A-6. These molecules are members of the dicyanomethylenedihydrofuran (DCDHF) class of single-molecule emitters that contain an amine electron donor and a DCDHF acceptor linked by a conjugated unit; DCDHF-N-6 and DCDHF-A-6 have naphthalene- and anthracene-conjugated linkers, respectively. These molecules maintain the beneficial photophysics of the phenylene-linked DCDHF (i.e., photostability, emission wavelength dependence on solvent polarity, and quantum yield sensitivity to solvent viscosity), yet offer absorption and emission at longer wavelengths that are more appropriate for cellular imaging. We demonstrate that these new fluorophores are less photolabile in an aqueous environment than several other commonly used dyes (rhodamine 6G, Texas Red, and fluorescein). Finally, we image single copies of the acene DCDHFs diffusing in the plasma membrane of living cells.

1. Introduction

Applying single-molecule techniques to biological problems is currently an active and growing field of research.13 Exploring biological activity within cells one molecule at a time makes it possible to detect hidden processes previously not seen or understood. It also becomes possible to reveal physiologically relevant heterogeneities obscured by the ensemble averaging of bulk measurements.48 However, these advantages can be realized only when using suitable fluorescent probes, and so there is a general effort to develop new and better emitters.913 For example, there is a need for small organic fluorophores that are brighter, more photostable, excitable in the green or red (in order to reduce cellular autofluorescence background signals), and ideally report a change in the environment with a corresponding detectible change in fluorescence. Here, we report the solvatochromic and photophysical properties of two red-emitting members of the dicyanomethylenedihydrofuran (DCDHF) class of single-molecule fluorophores currently being developed by our labs. (The nomenclature we use in this paper is “acceptor-linker-donor,” where the acceptor is the DCDHF structure, the linker is a conjugated ring—either phenylene P, naphthalene N, or anthracene A—and the donor is an amine with two alkyl chains with six carbons each. Note that this deviates slightly from previous nomenclature in which the phenyl linker was not specifically assigned, i.e., DCDHF-P-6 was formerly labeled “DCDHF-6.”).

As reported previously,1417 nonlinear optical chromophores that contain an amine donor and a DCDHF acceptor connected by a conjugated linker not only are robust single-molecule fluorophores but also exhibit sensitivity to solvent polarity and viscosity (local rigidity). The most extensively studied derivative from this class is DCDHF-P-6, whose donor and acceptor are conjugated by a 1,4-substituted phenyl ring (see Figure 1A):16 DCDHF-P-6 shows minimal fluorescence in solution—the result of an intramolecular twist on the excited-state manifold16,18,19 that introduces nonradiative relaxation pathways—but becomes very bright when constrained in rigid environments, when the nonradiative conformer is inhibited from forming. The primary benefit of this viscosity sensitivity is a fluorogenic label that brightens dramatically (“turns on”) upon binding to cellular components, producing an enhanced signal relative to the background fluorescence from unbound fluorophores. DCDHFP-6, however, is not ideal as a cellular label because it requires illumination at short wavelengths that also produce high background signal from cell autofluorescence.

Figure 1
(A) Structures of DCDHF-P-6, DCDHF-N-6, and DCDHF-A-6. (B) Equal concentrations of fluorophore in liquid (left) and frozen (right) solvent solutions illuminated by a handheld UV lamp (365 nm), with a 500 nm long-pass filter placed before the lens of a ...

To refine our probes for cellular imaging, it is necessary to retain the beneficial properties of DCDHF-P-6 while pushing absorption to longer wavelengths (>500 nm), where endogenous molecules such as flavins that contribute to cellular autofluorescence do not absorb.20 The most straightforward way to red-shift a dye is to lengthen its conjugation, so we have been developing several DCDHF derivatives with different conjugated linkers. For instance, one of the originally studied DCDHFs extended the conjugation using a styryl linker,15 which red-shifted the absorption and emission but also lowered the fluorescence quantum yield ΦF in solution (presumably by increasing the number of accessible twisted states by adding additional isomerization pathways). An alternative modification that red-shifts the absorption without adding additional nonradiative pathways extends the conjugation using acenes, which are more rigid than styryl groups.21 Here, we report that the derivatives DCDHF-N-6, which contains a 2,6-naphthyl linker between the donor and acceptor, and DCDHF-A-6, with a 2,6-anthryl linker (see Figure 1), not only maintain the solvatochromism and viscosity sensitivity characteristic of their phenyl-containing sister DCDHF-P-6 but also can be excited at 514 and 532 nm or longer (where cellular autofluorescence background is drastically suppressed).20,22

In a previous publication, the naphthyl DCDHF was used as a membrane probe: single copies were studied as lipid analogs to probe diffusion in the plasma membrane of eukaryotic cells.22 Here, we report the fundamental photophysical properties and environmental dependence of two acene DCDHFs in controlled conditions (e.g., in solvents with a range of polarities and in polymer films) in order to better judge and understand the full utility and potential of these probes.

2. Materials and Methods

2.1. Experimental Methods

Bulk solution absorption and emission spectra were acquired on a Perkin-Elmer Lambda 19 UV-vis spectrometer and a SPEX Fluoromax-2 fluorimeter using a standard 1 cm path length quartz cuvette. Fluorescence quantum yields were referenced against standards with known quantum yields, corrected for differences in optical density and solvent refractive index.23 DCDHF-P-6 and DCDHF-N-6 in toluene were measured against rhodamine 6G in ethanol (ΦF = 0.95); the quantum yield of DCDHF-A-6 in toluene was measured against Texas Red in ethanol (ΦF = 0.93).24 The fluorophores in other solvents were measured against their own values in toluene. All quantitative measurements were done at low concentrations (absorbance values less than 0.2) to avoid any complications with dimer or aggregate formation, although evidence thereof was not encountered even at higher concentrations (~10−4 M). Extinction coefficients were measured from dilutions of solutions with known concentrations.

For bulk spectra in polymer films, a small amount of highly concentrated dye solution was mixed into a 20% (by mass) solution of poly(methyl methacrylate) (PMMA, Tg = 105 °C, MW = 75 000 g/mol, atactic, polydispersity ~2.8, PolySciences Inc.) in toluene, then the solution was spin-cast onto a glass slide producing a film 20 µm thick as measured by ellipsometry. For absorption spectra, the slide was placed orthogonal to the beam with an undoped film in the reference channel; for emission spectra, the slide was placed in an orientation so as to reject the scattered excitation light. Absorption and emission were measured at several locations throughout the film to compensate for macroscopic heterogeneity in film thickness and coverage, which otherwise would result in variations in absorbance and emission signals.

Fluorescence-lifetime experiments were performed using a home-built scanning-stage confocal microscope based upon a commercial inverted microscope (Nikon TE300). Samples were excited using 532 nm illumination from an optical parametric oscillator (Coherent Mira-OPO) pumped by a mode-locked Ti: sapphire laser (Coherent Mira 900) with a repetition rate of 75 MHz and pulses 120 fs in duration; the Ti:sapphire was pumped by an Ar-ion laser (Coherent Innova 200). For two-photon fluorescence-lifetime measurements, 800 nm light was used directly from the mode-locked Ti:sapphire laser. Emitted photons passed through a 545 nm dichroic and two 545 nm long-pass filters and were detected with a single-photon avalanche photodiode (Micro Photon Devices PDM) equipped with a time-correlated single-photon counting data analysis board (PicoQuant TimeHarp200). The instrument response function (IRF) was recorded using scattered excitation light from a clean glass coverslip (the two long-pass filters were removed and OD filters were added to the beam path). The IRF was deconvolved from the data and then fit using a maximum-likelihood estimator.

Samples for quantitative single-molecule studies were prepared in 1% (by mass) solutions of PMMA in distilled toluene doped with nanomolar fluorophore concentrations; these solutions were then spin-cast onto plasma-etched glass coverslips to produce films 30 nm thick as measured by ellipsometry. (The solvent distillation and plasma treatment of the substrates is done to remove spurious sources of fluorescence.) Samples were studied using a Nikon Diaphot 200 inverted microscope in an epifluorescence configuration25 using 532 nm light from a continuous wave doubled Nd:YAG laser (Spectra-Physics Millenia); the intensity at the sample was between 0.25 and 1.7 kW/cm2. The emission was collected through a 100×, 1.4 NA oil immersion objective, filtered using a 545 nm dichroic and 550 nm long-pass filter to remove scattered excitation light, and imaged onto a back-illuminated, frame-transfer Si CCD camera (Roper Scientific MicroMAX) with an integration time of 100 ms.

Sequences of single-molecule images (i.e., movies) were used to extract the total number of detected photons before photo-bleaching, where all the photons (minus background) contributing to a single-molecule spot were spatially and temporally integrated. Even though the Micro MAX Si CCD does not have electron multiplication, the conversion gain (defined as the number of A-to-D converter counts per photoelectron) is 0.521 counts/electron for our camera; the number of detected photons is equal to ADC counts divided by conversion gain. It is also useful to calculate the number of emitted photons by correcting the measured value of detected photons using the collection efficiency of our setup (D = ηQFcollFoptFfilter), which is the product of the camera quantum efficiency ηQ, the angular collection factor Fcoll determined by the objective NA, the transmission factor through the objective and microscope optics Fopt, and the transmission factor through the various filters Ffilter, respectively.25 At the emission wavelengths, ηQ = 75–80% for our camera, the maximum possible Fcoll for our setup is 38% for a single dipole emitter aligned horizontally;26 we measured Fopt for our setup to be 50%, and we measured Ffilter to be 60–70% for the filter set we used. This yields DDCDHF-N-6 = 9.1% and DDCDHF-A-6 = 9.9%.

Samples for aqueous bulk photostability measurements and single-molecule images were prepared using 5% (by mass) gelatin (type A, Bloom ~200, MP Biomedicals) in purified water. The gelatin solution was liquefied at 37 °C. A small volume (π.5 µL) of dye stock solution in dimethyl sulfoxide was mixed with 10 µL of gelatin, sandwiched between two glass coverslips, and allowed to gel at room temperature. Samples were imaged using the microscope and CCD camera setup described above. Fluorescein (unmodified parent version) was measured using a 488 nm laser (Novalux Protera 488-20), DCDHF-N-6 and Texas Red were imaged using 532 nm, and DCDHF-A-6 was imaged using a 594 nm HeNe laser.

Movies of DCDHFs in the plasma membrane were recorded using a back-illuminated, frame-transfer, electron-multiplication Si EMCCD camera (Andor iXon or Roper Cascade). Samples were made by incorporating the probe into Chinese hamster ovary (CHO) cells as follows. A fluorophore film was made by drying 5 µL of an 80 µM solution of DCDHF-A-6 in chloroform onto a glass coverslip. This film was reconstituted with 50 µL of ethanol; 4 µL of this ethanol solution was added to 400 µL of RPMI 1640 cell medium without phenol red (Gibco). The cells were incubated with 440 µL of ~80 nM final dye/RPMI solution for 20 min at 37 °C. The solution was then aspirated and the cells were gently washed one time with RPMI 1640 medium which was then replaced by the CHO cell imaging buffer. (For further experimental details, components of the CHO imaging buffer, and imaging conditions for DCDHF-N-6, see ref 22.)

2.2. Synthesis

Samples of DCDHF-P-6 (2-dicyanomethylene-3-cyano-5,5-dimethyl-4-(4′-dihexylaminophenyl)-2,5-dihydrofuran) and DCDHF-N-6 (2-dicyanomethylene-3-cyano-5,5-dimethyl-4-[6-(dihexylamino)naphthalene-2-yl]-2,5-dihydrofuran) were synthesized as reported elsewhere.22,27,28 The synthesis of DCDHF-A-6 (2-dicyanomethylen-3-cyano-5,5-dimethyl-4-(6-dihexylaminoanthracen-2-yl)-2,5-dihydrofuran) has not been previously described and is summarized in Scheme 1. The critical precursor 2-bromo-6-dihexylaminoanthracene 121 was lithiated with n-BuLi at −78 °C over 1 h and trapped with TMS-protected acetone cyanohydrin. The resulting α-siloxyimine intermediate was hydrolyzed with 2 M aqueous hydrochloric acid at room temperature to afford the crude α-ketol 2, which was used directly in the DCDHF ring preparation. By this route DCDHF-A-6 was prepared in overall 37% yield.29

SCHEME 1
Synthesis of DCDHF-A-6

A measured amount of 2-bromo-6-dihexylaminoanthracene (0.42 g, 0.95 mmol) was added with 15 mL of anhydrous THF to a 100 mL round-bottom flask. The mixture was cooled to −78 °C, and n-BuLi in hexane (2.5 M, 0.46 mL, 1.15 mmol) was added with stirring over 10 min. The resulting mixture was stirred for an additional 1 h at −78 °C, and then 2-methyl-2-trimethylsilyloxypropionitrile30 (0.80 g, 5.0 mmol) was added to the resulting mixture via syringe over 5 min. After completion of addition, the temperature was allowed to increase gradually to room temperature and the reaction mixture was stirred overnight at room temperature. The reaction was quenched with 10 mL of 2 M hydrochloric acid for 6 h and extracted with diethyl ether. The organic layer was washed with water and brine, and dried with anhydrous MgSO4 Solvent was removed by rotary evaporation giving the crude product, which was mixed with malononitrile (2.0 g, 30 mmol), pyridine, 15 mL, and 5 drops of acetic acid. This mixture was stirred for a day at room temperature, an additional day at 45 °C, and then poured into cold 5% hydrochloric acid and extracted with dichloromethane. The organic solution was washed with water and brine and dried with anhydrous MgSO4. Solvent was removed by rotary evaporation, and the residue was purified by silica gel flash chromatography with hexane and hexane/ethyl acetate mixture (up to 5:1) as eluents to give 200 mg of green solid (37% yield in two steps). 1HNMR (400 MHz, CDCl3) δ 8.60 (d, J = 2.0 Hz, 1H), 8.38 (s, 1H), 8.06 (s, 1H), 7.93 (dd, J = 9.3, 3.2 Hz, 2H), 7.81 (dd, J = 9.1, 2.0 Hz, 1H), 7.27 (dd, J = 9.5, 2.4 Hz, 1H), 6.88 (d, J = 2.4 Hz), 3.48 (t, J = 7.8 Hz, 4H), 1.96 (s, 6H), 1.77-1.67 (m, 4H), 1.47-1.34 (m, 12H), 0.95 (t, J = 7.0 Hz, 6H). 13C NMR (100 Hz, CDCl3) δ 176.1, 175.6, 147.7, 137.4, 134.2, 132.9, 130.4 (2C), 129.0, 127.2, 127.0, 122.0, 121.8, 121.0, 118.5, 112.1, 111.9, 111.1, 102.1, 98.5, 97.5, 57.0, 51.3, 31.7, 27.5, 27.3, 26.8, 22.7, 14.1. MS (APCI) m/z 545.0. UV-vis (CH2Cl2) λ1 = 296 nm, ϵ1 = 4.25 × 104 L cm−1 mol−1, λ2 = 378 nm, ϵ2 = 2.51 × 104 L cm−1 mol−1, λ3 = 397 nm, ϵ3 = 2.47 × 104 L cm−1 mol−1, λ4 = 619 nm, ϵ4 = 3.38 × 104 L cm−1 mol−1. Anal. Calcd for C36H40N4O: C, 79.38; H, 7.40; N, 10.29. Found: C, 79.00; H, 7.57; N, 10.35.

3. Results and Discussion

3.1. Bulk Photophysical Properties

As mentioned above, a key goal of this work is to identify derivatives in the DCDHF class with long-wavelength absorption and emission where cellular autofluorescence is diminished. In most cases, pumping with wavelengths of 488 nm or shorter produces strong emission from essential cellular components such as flavins.20 As can be seen in Figure 2 and Table 1, in contrast to DCDHF-P-6, the new acene DCDHFs can be efficiently pumped using green or yellow light (e.g., 532 nm) and exhibit large Stokes shifts, further enabling filtering to reject pumping light. Moreover, a full study of DCDHF-N-6 and DCDHF-A-6 solvent photophysics reveals that the reporter functions discovered in DCDHF-P-6 apply to the red-shifted dyes. Below we discuss the properties of these fluorophores that are beneficial for cellular imaging (including turn-on fluorescence upon rigidization, strong polarity sensitivity of emission wavelength and fluorescence lifetime, and bright emission), which make the red DCDHFs useful for various imaging modalities.

Figure 2
Normalized absorption and fluorescence emission spectra of DCDHF-P-6, DCDHF-N-6, and DCDHF-A-6 in toluene. Absorption for DCDHF-N-6 is at long enough wavelengths for excitation at 514 or 532 nm, thus avoiding much of the cellular autofluorescence; DCDHF-A-6 ...
TABLE 1
Spectral Parameters of DCDHF-P-6, DCDHF-N-6, and DCDHF-A-6 in a Representative Range of Liquid Solventsa

As is typical of many other DCDHF derivatives we have explored, the acene DCDHFs exhibit sensitivity to solvent viscosity: the fluorescence quantum yield increases with increasing local rigidity. For example, compare the quantum yields in solution and in polymer listed in Table 1 and note the increased brightness upon freezing solutions seen in Figure 1B. The previously proposed16 mechanism for this viscosity dependence involves a twist of the dicyanomethylene double bond, which introduces a nonradiative relaxation pathway via a twisted intramolecular charge-transfer (TICT)18 state. In solvents with low viscosity, the excited-state manifold is able to relax via bond twists to the TICT state, an increased electronic charge separation that is stabilized especially well by polar solvents (hence the lower quantum yield in polar solvents); in rigid environments, this twisting is kinetically less favorable, thus reducing the nonradiative contribution to the relaxation pathways and increasing the fluorescence quantum yield. This hypothesis was developed by performing Hartree-Fock and semiempirical quantum-chemistry calculations on the phenyl-conjugated DCDHF-P-6 derivative.16 Repeating these calculations on DCDHF-N-6 showed no significant departure from previous results (data not shown). While these calculations suggest that the sensitivity to local environment originates from the same intramolecular twist, they do not provide any insight into the enhanced quantum yield of the acene dyes over DCDHF-P-6; however, a mechanism could be revealed by a full photophysical study of the relaxation pathways, including a more extensive computational study.

Viscosity sensitivity for DCDHF-N-6 is quantified experimentally in the data in Table 2 and Figure 3A, which plots the logarithm of quantum yield versus the logarithm of solvent viscosity. Just as was previously seen with DCDHF-P-6,16 the alcohol solvents show a definite correlation between DCDHF-N-6 emission probability and solvent viscosity, whereas other solvents do not exhibit a single trend. (As a side comment, we note that the high quantum yield of DCDHF-N-6 in toluene and benzene as compared to cyclohexane in Table 2 is observed to some degree in all DCDHF derivatives studied and is likely due to some specific solvent interactions-possibly π-π stacking between the solvent and the fluorophores-that restricts intramolecular rotation pathways.)

Figure 3
Environment-sensitivity spectroscopic data from Table 2 and Table 3. (A) Log of fluorescence quantum yield as a function of the log of viscosity (relative to water η = 1.01 cP) for DCDHF-N-6. The data for the fluorophore in alcohols are fit by ...
TABLE 2
Photophysical and Solvatochromic Properties of DCDHF-N-6a

The small viscosity range in Figure 3A covers only one decade, so it does not represent the large increase in quantum yield that occurs in more rigid environments: the plot is only a small portion of the dynamic range. Figure 1B better illustrates the dramatic jump in brightness as a solution of DCDHFs in liquid solvent is frozen to form a rigid environment. To partially quantify the full increase of fluorescence, we measured the quantum yield of DCDHF-N-6 in a PMMA polymer film to be nearly unity, while that in ethanol is only 1.7% (see Table 1). A similar increase in the fluorescence is demonstrated for DCDHF-A-6 in Table 1. We attribute this jump in brightness to the drastic change in viscosity, given that PMMA is still quite polar, approximately equivalent to dimethyl sulfoxide.

In solution, the acene fluorophores exhibit strong solvato-chromism in addition to the viscosity sensitivity discussed above. Table 2 and Table 3 list absorption and emission maxima, fluorescence quantum yield, and molar extinction coefficient (ϵmax) for DCDHF-N-6 and DCDHF-A-6 measured in various solvents. To explore the dependence of the Stokes shift on the solvent polarity, we use the Lippert formalism for general solvent polarity effects.23 Recalling that DCDHFs have an asymmetric donor-acceptor motif, it is reasonable to expect changes in the molecular dipole moment upon excitation: the charge-transfer state can be stabilized by solvent dipoles rearranging around the excited fluorophore; thus, the extent of stabilization depends on the polarity of the solvent. In the Lippert approximation the orientation polarizability Δf is used as a parameter to represent the degree of molecular rearrangement around a dipole in a continuous medium leading to the observed Stokes shift:

equation M1
(1)

equation M2

and h is Planck’s constant, c is the speed of light in a vacuum, a is the Onsager cavity radius (assumed to be ~5 Å), and µE and µG are the excited- and ground-state dipole moments, respectively. The Lippert plots in Figure 3B show the Stokes shift versus Δf, and from the slopes we calculate the change in the dipole moment upon excitation to be |µE - µG| = 9.4 D for DCDHF-N-6 and 9.7 D for DCDHF-A-6. These values are significantly greater than the value for DCDHF-P-6 (4.4 D);16 in other words, the acene derivatives exhibit stronger sensitivity to solvent polarity than their phenyl sister. Moreover, because DCDHF-N-6 and DCDHF-A-6 exhibit red-shifts in emission of over 100 nm going from nonpolar to polar media (see Table 1), it should be possible to record emission spectra to harness this reporter function so as to monitor polarity changes in the immediate local environment of single molecules.33

TABLE 3
Solvatochromatic Properties of DCDHF-A-6a

Fluorescence-lifetime imaging microscopy34 is another imaging modality available using DCDHF molecules. Table 4 shows that both the one-photon excited and two-photon excited (in brackets) fluorescence lifetimes of bulk DCDHF-N-6 samples depend on the solvent or environment in which the fluorophore resides. The one-photon lifetime increases from below the instrument response time in polar ethanol to a few nanoseconds in nonpolar toluene and even higher in PMMA; the two-photon lifetime exhibits a similar increase going from solution to polymer. The increase in lifetime can be correlated to the increase in quantum yield and is most likely the result of suppressing the nonemissive TICT relaxation channel in toluene and PMMA. This property could be used to follow dynamic changes in local environment on the single-molecule level or to map different regions within a sample, using fluorescence lifetime as contrast.

TABLE 4
Sensitivity of DCDHF-N-6 Fluorescence Lifetime (τF) to Different Environmentsa

3.2. Single-Molecule Properties

A strong test of the utility of a fluorophore is its ability to be imaged at the single-molecule level, which requires bright fluorescence, weak coupling with dark states, and robust photostability. The acene DCDHFs are superb single-molecule emitters: for example, single copies of DCDHF-A-6 and DCDHF-N-6 in films of aqueous protein gels are easily visible as shown in Figure 4, parts A and B, wide-field epifluorescence images of typical samples.

Figure 4
(A) Surface plot of emission from single DCDHF-N-6 molecules in a gelatin film, with 2 × 2 Gaussian smoothing. For this epifluorescence image, the excitation wavelength was 532 nm, the intensity at the sample was approximately 0.25 kW/cm2, and ...

To better characterize the photostability of a single-molecule emitter using one simple parameter, we recorded the distribution of the number of photons emitted from single fluorophores before photobleaching, Ntot,emitted (see Table 1). This parameter assesses a fundamental property of the emitter because it should not depend on excitation intensity (in the absence of saturation or nonlinear effects): if the pumping intensity is selected to produce a desired emission rate, this parameter assesses how long a molecule will survive on average. From wide-field epifluorescence movies, 193 DCDHF-N-6 and 135 DCDHF-A-6 single-molecule traces (similar to Figure 4C, inset) were recorded, inspected for digital bleaching, background-subtracted, and integrated to build a distribution (Figure 4C). These Ntot,detected distributions exhibit the expected exponential shape for a first-order photobleaching process, and single-exponential fits yield 130 000 photons detected per DCDHF-N-6 molecule on average and 226 000 photons detected per DCDHF-A-6. Converting to the number emitted using the detection efficiency D, we find Ntot,emitted = 1.4 × 106 photons emitted per DCDHF-N-6 molecule on average and 2.2 × 106 per DCDHF-A-6, which approach the values of DCDHF-P-6 and rhodamine 6G (2.4 × 106 and 1.9 × 106 photons emitted per molecule, respectively),15,24 both of which are demonstrably good single-molecule fluorophores.

3.3. Bulk Photostability

The single-molecule Ntot,emitted value for DCDHF-N-6 is comparable to the value determined using the bulk photobleaching behavior of high-concentration samples of DCDHF-N-6 in PMMA. To perform the ensemble-averaged measurement, plots of integrated, background-subtracted emission intensities versus time for bulk samples were fit using two exponentials, and the time constant τB was extracted from these photobleaching curves.35 The number of photons emitted was calculated from the inverse of the photobleaching quantum yield using the absorption cross section σ532 of DCDHF-N-6 (1.8 × 10−16 cm2), the illumination intensity I532, and the quantum yield:

equation M3
(2)

where Nabs is the number of photons absorbed, Rabs is the rate of photon absorption, h is Planck’s constant, and c is the speed of light in vacuum. This technique is independent of the photon losses in the microscope, so it is a separate corroboration of the single-molecule measurement. A bulk measurement yielded Ntot,emitted = 2.6 × 106 photons emitted per DCDHF-N-6 molecule, comparable to the single-molecule value of 1.4 × 106; if anything, the single-molecule value may be an under-estimate. As an independent check of this method of calculating Ntot,emitted, we measured the photons emitted per rhodamine 6G molecule in polyvinyl alcohol to be 1.4 × 106, which is comparable to the published24 value of 1.9 × 106 photons emitted per rhodamine 6G molecule in ethanol.

To compare the photostability of the acene DCDHFs to other commonly used cellular fluorophores in a cell-like model environment, we measured the photobleaching quantum yield (ΦB) in an aqueous protein gel. ΦB is the probability that a single molecule will photobleach after absorbing a photon, so the smaller the value of ΦB, the more total photons are available for detection. We define the photobleaching quantum yield as

equation M4
(3)

where nbleach is the number of molecules bleached after a measured time and Rbleach is the bleaching rate. The time constant τB was found by fitting the bleaching curves of the different fluorophores using three exponentials with a vertical offset to account for the background; τB is the weighted average of the three time constants. Each fluorophore was measured at several different illumination intensities, all below saturation. Our measured values for R6G and Texas Red ΦB are similar to their respective literature values in water.24 Average values for all measured ΦB are displayed in Table 5; the acene DCDHFs prove to be many times more photostable than rhodamine 6G, Texas Red, and fluorescein in this aqueous environment.

TABLE 5
Acene DCDHFs Perform Well in a Comparison of Photobleaching Quantum Yields in Aqueous Gelatin

The acene DCDHFs not only are resilient to photobleaching, they also resist entering long-lived dark states: most DCDHF-N-6 and DCDHF-A-6 molecules exhibited little or no blinking on the time scale of our experiments (i.e., 100 ms integration time, movies lasting tens of seconds), as seen in the sample time-trace inset of Figure 4C.

3.4. Cellular Imaging

The ultimate test of our fluorophores is whether they can be imaged on the single-molecule level in a cellular environment long enough to report on some process of interest. Recently, we successfully used several different DCDHF derivatives as fluorescent lipid analogs as tracers to probe cellular membrane dynamics.22 Single copies of the naphthyl derivative (both DCDHF-N-6 and DCDHF-N-12, a version with longer alkyl chains on the amine donor) were visualized diffusing in the plasma membrane of mammalian CHO cells (Figure 5A). We were also able to image single copies of DCDHF-A-6 in CHO cell membranes (Figure 5B). In the future, with further optimization of the DCDHF class, we hope to design additional high-performance reporter molecules capable of detection at the single-molecule level in cells, but already DCDHF-N-6 emits as many photons and can be imaged with a signal-to-noise ratio comparable to that of a popular lipid-analog fluorophore, Tritc-DHPE (a tetramethyl-rhodamine derivative), as reported in detail in ref 22.

Figure 5
Epifluorescence images of acene DCDHFs in living CHO cells. (A) Image of single DCDHF-N-6 molecules diffusing in a region of a CHO plasma membrane, with 2 × 2 Gaussian smoothing. The excitation wavelength was 532 nm, the intensity at the sample ...

4. Conclusion

This work has described two acene relatives of the earlier-reported DCDHF-P-6 molecule, DCDHF-N-6 and DCDHF-A-6, which exhibit strong fluorescence upon pumping at the longer wavelengths that are necessary for single-molecule imaging in cells. These two long-wavelength fluorophores also exhibit the strong emission and weak dark states which give rise to single-molecule properties approaching those of DCDHF-P-6 and rhodamine 6G when imbedded in a PMMA matrix and superior photostability in an aqueous protein environment.

Further experiments involving DCDHF-N-6 and DCDHF-A-6 can take advantage of other properties of the DCDHF class of fluorophores that these acene derivatives possess, such as measurable shifts in emission wavelength or fluorescence lifetime with variations in local polarity. Such experiments include protein labeling using maleimide covalent binding and FRET pairing with other fluorophores or other DCDHF derivatives. In some biological experiments, it may be necessary to impart better water solubility to these fluorophores, which can be achieved by adding alcohol, carboxylic acid, or sulfonic acid functional groups to the structure (either off the donor or acceptor).36 These first results—minimized background fluorescence from free fluorophores in solution, long-wavelength absorption and emission, high photostability in aqueous environments, and large solvatochromism—demonstrate that acene-linked DCDHF fluorophores are high-quality single-molecule emitters for cellular imaging.

Acknowledgment

The authors thank Yuan Li for assistance with calculations of the electronic structure of DCDHF-N-6, Dr. Frank Jäckel for ellipsometry measurements, Kit Werley for his suggestion to use eq 2, and Professor Jörg Enderlein for discussion about objective collection efficiency. This research was supported in part by the National Institutes of Health through the NIH Roadmap for Medical Research, Grant No. 1P20-HG003638.

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