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Neurodegener Dis. 2008 March; 5(3-4): 222–224.
Published online 2008 March 6. doi:  10.1159/000113708
PMCID: PMC2677749
NIHMSID: NIHMS109989

Leucine-Rich Repeat Kinase 2 Colocalizes with α-Synuclein in Parkinson's Disease, but Not Tau-Containing Deposits in Tauopathies

Abstract

Background

Mutations in leucine-rich repeat kinase 2 (LRRK2) are thus far the most frequent genetic cause associated with autosomal dominant and idiopathic Parkinson's disease.

Objective

To examine whether LRRK2 is directly associated with the pathological structures of Parkinson's disease, dementia with Lewy bodies, and other related disorders using highly specific antibodies to LRRK2.

Results

LRRK2 antibodies strongly labeled brainstem and cortical Lewy bodies, the pathological hallmarks of Parkinson's disease and dementia with Lewy bodies, respectively. We found that 20–100% (mean 60%) of α-synuclein-positive Lewy bodies contained LRRK2. While antibodies raised against various regions of LRRK2 were previously shown to label recombinant LRRK2 on Western blots, only antibodies raised against the N- and C-termini, but not the regions containing folded protein domains of LRRK2, immunolabeled Lewy bodies. In Alzheimer's disease, Hirano bodies were found to contain LRRK2 and the neurofibrillary tangles in progressive supranuclear palsy remained unlabeled.

Conclusions

Information on the cellular localization of LRRK2 under normal and pathological conditions will deepen our understanding of its functions and molecular pathways relevant to the progression of Parkinson's disease and related disorders.

Key Words: Lewy bodies, Leucine-rich repeat kinase 2, Parkinson's disease, Synuclein

Introduction

Approximately 1–2% of individuals aged 65 years and older are affected by Parkinson's disease (PD), a condition characterized by selective loss of dopaminergic neurons and the presence of Lewy bodies (LBs), the hallmark neuropathology of the disease, in the substantia nigra. Mutations in genes encoding parkin, PINK1, and DJ-1 are correlated with autosomal recessive, early-onset familial PD, whereas genes encoding α-synuclein and leucine-rich repeat kinase 2 (LRRK2) are linked to autosomal dominant PD. LRRK2 is a risk factor for both familial and sporadic PD, with the pathogenic G2019S mutation accounting for up to 6% of familial and 2% of sporadic cases [1]. Very recently, we have demonstrated that LRRK2, in addition to α-synuclein and parkin [2], is the third component of LBs [3, 4]. In this study, we analyzed the spatial relationship between LRRK2 and α-synuclein as well as tau in PD and other neurodegenerative diseases.

Materials and Methods

Brain samples were obtained from patients with confirmed PD (n = 5; aged 53–76 years), dementia with Lewy bodies (DLB; n = 6; aged 68–85 years), Alzheimer's disease (AD; n = 4; aged 69–82 years), progressive supranuclear palsy (PSP; n = 2; aged 76 and 83 years) and from control subjects (n = 4; aged 42–79 years). Tissues were fixed in formalin, embedded in paraffin and immunostained using 4 different highly specific antibodies against LRRK2 as previously described [3].

Results

Using antisera to 4 different regions of the LRRK2 protein, all of which could recognize LRRK2 from normal and PD brain homogenates by Western blot [3], only 2 with epitopes outside the 5 folded domains consistently and specifically labeled LBs in all PD cases studied [4]. Similar to α-synuclein, both rim and core regions of brainstem LBs were detected by anti-LRRK2 (fig. (fig.1).1). LBs stained for LRRK2 and α-synuclein on adjacent sections revealed significant overlap (fig. 1a, b and insets). Quantification of the total number of LBs immunostained in sections of the brainstem from 5 cases of PD showed that LRRK2-positive LBs stained 20–100% (mean 60%) of those positive for α-synuclein in adjacent sections. Small neuritic processes containing α-synuclein were rarely labeled by anti-LRRK2 (fig. (fig.1b,1b, arrowheads). In cortical regions of AD and 2 DLB cases, occasional morphologically characteristic Hirano bodies and neuronal cytoplasm were positive for LRRK2 (fig. (fig.1c),1c), but never neurofibrillary tangles nor amyloid deposits (not shown). In PSP, tangles, while intensely immunolabeled for tau, were negative for LRRK2 (not shown). In control brains, weak and uniform staining of neuronal cytoplasm was observed [3].

Fig. 1.
In adjacent sections of PD brainstem, the same LBs often contain both LRRK2 (a) and α-synuclein (b, arrows). Insets show a higher magnification of representative LBs. In AD hippocampus, Hirano bodies (arrows) are also found to contain LRRK2 ( ...

Discussion

Using a battery of specific antisera, we have demonstrated LRRK2 staining of LBs in the brainstem in idiopathic PD and in the cortex in DLB with 2 antibodies detecting the sequence outside the 5 folded domains, indicating that LRRK2 is indeed a component of LBs in these 2 diseases [3, 4]. Initially, a negative finding was reported using an antibody detecting the sequence within the folded LRR domain [5]; however, our positive findings [3, 4] were subsequently confirmed by others [6, 7]. Here, we demonstrate a significant overlap of α-synuclein-positive LBs with LRRK2. This is unlikely due to nonspecific cross-reactivity of the antibodies since the specificity of these two antibodies was demonstrated in our studies and also confirmed by other groups [3, 4, 6, 8]. The differential detection of antibodies against different regions in the same protein is probably due to the inherent protein structure which results in the exposure or masking of specific epitopes, particularly for large proteins like LRRK2.

LRRK2 is predicted to contain several functionally important domains including mitogen-activated protein kinase and recent reports have confirmed that LRRK2 functions as a kinase and pathogenic mutations augment its activity in cell models [9]. Since the in vitro phosphorylation of α-synuclein promotes its aggregation [10], the colocalization of the protein kinase LRRK2 and α-synuclein in the same LBs may provide a molecular mechanism associated with parkinsonism, including aberrant protein phosphorylation and subsequent aggregation. While we find no colocalization of LRRK2 with tau protein in AD and PSP cases prepared identically to the LRRK2-positive PD cases with all 4 antibodies used in these studies, another group using different fixation protocols [7] found LRRK2 in neurofibrillary tangles in AD with 1 of these antibodies. It is unclear what causes the difference between our observations.

Of interest is the presence of LRRK2 in Hirano bodies in both AD and DLB cases. Since Hirano bodies contain F-actin and LRRK2 contains the Roc/GTPase domain, LRRK2 involvement in the regulation of cytoskeletal structures merits further investigation.

Acknowledgements

This project was supported in part by Alzheimer's Association, National Institute on Aging, National Parkinson Foundation, Johnson & Johnson Corporate Office of Science and Technology, Philip Morris USA Inc., and Philip Morris International.

References

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Articles from Neuro-Degenerative Diseases are provided here courtesy of Karger Publishers