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J Clin Microbiol. 1989 August; 27(8): 1893–1895.
PMCID: PMC267694

Sensitivity and specificity of pooled versus individual sera in a human immunodeficiency virus antibody prevalence study.


We evaluated the efficacy of testing pooled versus individual sera for the detection of human immunodeficiency virus antibody. A total of 5,000 individual specimens and 500 pools of 10 specimens each were assayed by an enzyme-linked immunosorbent assay. There was complete agreement in human immunodeficiency virus enzyme-linked immunosorbent assay reactivity for pooled versus individual specimens. An estimated savings of 60 to 80% (labor and supplies) can be realized dependent upon pooling and assay format.

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