Search tips
Search criteria 


Logo of jcmPermissionsJournals.ASM.orgJournalJCM ArticleJournal InfoAuthorsReviewers
J Clin Microbiol. 1989 July; 27(7): 1661–1663.
PMCID: PMC267636

Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome.


In four cases of pulmonary toxoplasmosis occurring in patients with acquired immunodeficiency syndrome, Toxoplasma sp. was discovered in bronchoalveolar-lavage fluid (three cases) and in lung biopsy specimen (one case) by using the following methods: direct examination of smears stained with eosine-methylene blue fast stain, indirect immunofluorescence assay, and inoculation of MRC5 fibroblast cell line in tissue culture.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (420K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Catterall JR, Hofflin JM, Remington JS. Pulmonary toxoplasmosis. Am Rev Respir Dis. 1986 Apr;133(4):704–705. [PubMed]
  • Derouin F, Mazeron MC, Garin YJ. Comparative study of tissue culture and mouse inoculation methods for demonstration of Toxoplasma gondii. J Clin Microbiol. 1987 Sep;25(9):1597–1600. [PMC free article] [PubMed]
  • Desmonts G, Naot Y, Remington JS. Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired Toxoplasma infections. J Clin Microbiol. 1981 Nov;14(5):486–491. [PMC free article] [PubMed]
  • Gill VJ, Nelson NA, Stock F, Evans G. Optimal use of the cytocentrifuge for recovery and diagnosis of Pneumocystis carinii in bronchoalveolar lavage and sputum specimens. J Clin Microbiol. 1988 Sep;26(9):1641–1644. [PMC free article] [PubMed]
  • Luft BJ, Remington JS. AIDS commentary. Toxoplasmic encephalitis. J Infect Dis. 1988 Jan;157(1):1–6. [PubMed]
  • Mendelson MH, Finkel LJ, Meyers BR, Lieberman JP, Hirschman SZ. Pulmonary toxoplasmosis in AIDS. Scand J Infect Dis. 1987;19(6):703–706. [PubMed]
  • Murray JF, Felton CP, Garay SM, Gottlieb MS, Hopewell PC, Stover DE, Teirstein AS. Pulmonary complications of the acquired immunodeficiency syndrome. Report of a National Heart, Lung, and Blood Institute workshop. N Engl J Med. 1984 Jun 21;310(25):1682–1688. [PubMed]
  • Niel G, Desmonts G, Gentilini M. Immunofluorescence quantitative et diagnostic sérologique de la toxoplasmose: introduction des unités internationales dans l'expression des positivés. Pathol Biol (Paris) 1973 Feb;21(2):157–161. [PubMed]
  • Pintozzi RL. Modified Grocott's methenamine silver nitrate method for quick staining of Pneumocystis carinii. J Clin Pathol. 1978 Aug;31(8):803–805. [PMC free article] [PubMed]
  • Touboul JL, Salmon D, Lancastre F, Mayaud C, Fermand JP, Fouret P, Akoun G. Pneumopathie à Toxoplasma gondii chez un patient atteint de syndrome d'immunodépression acquis: mise en évidence du parasite par lavage bronchiolo-alvéolaire. Rev Pneumol Clin. 1986;42(3):150–152. [PubMed]
  • Tourani JM, Israël-Biet D, Venet A, Andrieu JM. Unusual pulmonary infection in a puzzling presentation of AIDS. Lancet. 1985 Apr 27;1(8435):989–989. [PubMed]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)