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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Exp Brain Res. Author manuscript; available in PMC 2010 May 1.
Published in final edited form as:
PMCID: PMC2675666
NIHMSID: NIHMS97351

Responses of Thoracic Spinal Interneurons to Vestibular Stimulation

Abstract

Vestibular influences on outflow from the spinal cord are largely mediated via spinal interneurons, although few studies have recorded interneuronal activity during labyrinthine stimulation. The present study determined the responses of upper thoracic interneurons of decerebrate cats to electrical stimulation of the vestibular nerve or natural stimulation of otolith organs and the anterior and posterior semicircular canals using rotations in vertical planes. A majority of thoracic interneurons (74/102) responded to vestibular nerve stimulation at median latencies of 6.5 msec (minimum of ~3 msec), suggesting that labyrinthine inputs were relayed to these neurons through trisynaptic and longer pathways. Thoracic interneuronal responses to vertical rotations were similar to those of graviceptors such as otolith organs, and a wide array of tilt directions preferentially activated different cells. Such responses were distinct from those of cells in the cervical and lumbar enlargements, which are mainly elicited by ear-down tilts and are synchronous with stimulus position when low rotational frequencies are delivered, but tend to be in phase with stimulus velocity when high frequencies are employed. The dynamic properties of thoracic interneuronal responses to tilts were instead similar to those of thoracic motoneurons and sympathetic preganglionic neurons. However, the preferred tilt directions of the interneurons were more heterogeneous than thoracic spinal outputs, showing that the outputs do not simply reflect an addition of local interneuronal activity.

Keywords: Otolith organ, vertical semicircular canal, vestbulo-autonomic responses, cardiovascular, respiration, sympathetic nervous system, spinal cord

Introduction

Since the earliest studies that mapped the efferents of the vestibular nuclei, it was appreciated that most vestibulospinal projections terminate on interneurons and not directly on motoneurons (Nyberg-Hansen 1964; Nyberg-Hansen and Mascitti 1964; Petras 1967; Wilson and Melvill Jones 1979). As such, spinal interneurons are likely involved in integrating descending vestibular signals with segmental and other inputs, and thus play a large role in the process of sensorimotor transformation. However, few studies have considered the responses of spinal interneurons to vestibular stimulation, and most of this work was focused on interneurons in the cervical or lumbar cord that likely regulate limb movements (e.g., Suzuki et al. 1985; Schor et al. 1986). The limb muscles in cats are activated during side-to-side tilts (roll), with extensor muscles contracting during ear-down rotations to produce righting responses (Money and Scott 1962; Roberts 1978; Wilson and Melvill Jones 1979; Wilson et al. 1986). At low frequencies of sinusoidal rotation, the responses of limb muscles are synchronous with stimulus position, but tend to be in phase with stimulus velocity when high rotational frequencies are employed (Wilson et al. 1986). Interneurons in the lower cervical and lumbar spinal cord have analogous response characteristics (Suzuki et al. 1985; Schor et al. 1986), suggesting that a bulk of the signal integration required to generate appropriate reflexes has been accomplished before the motor commands reach limb motoneurons.

Recent studies have also shown that motor and autonomic outputs from the thoracic spinal cord are influenced by vestibular signals. Motoneurons in the thoracic cord that innervate back, abdominal, and intercostal muscles respond to electrical and natural stimulation of labyrinthine afferents (Wilson et al. 1970a; Wilson et al. 1970b; Yates et al. 1993; Rossiter et al. 1996; Brophy et al. 1997). In addition, the firing of sympathetic nervous system efferents arising from the thoracic spinal cord is altered by vestibular stimulation (Uchino et al. 1970; Yates et al. 1993; Yates and Miller 1994; Ray et al. 1997; Kerman et al. 2000; Zakir et al. 2000; Voustianiouk et al. 2006). Unlike the activity of limb motoneurons, motor and sympathetic outflow from the thoracic spinal cord is modulated by fore-aft (pitch) tilt (Yates and Miller 1994; Rossiter et al. 1996; Brophy et al. 1997). Furthermore, the responses are distinct from those of limb muscles in being synchronous with stimulus position when both low- and high-frequency tilts are delivered (Yates and Miller 1994; Rossiter et al. 1996; Brophy et al. 1997). Such effects are needed to stiffen the trunk during changes in body orientation about the transverse axis (Rossiter et al. 1996; Brophy et al. 1997) and to generate the alterations in regional blood flow required during such postural alterations (Yates and Miller 1994).

If integration and processing of vestibular signals in the thoracic spinal cord mainly occurs at the level of interneurons, it is expected that local interneurons and thoracic outputs would have responses to tilts with similar spatial and temporal characteristics. Such a finding, in combination with previous data from lumbar and cervical interneurons (Suzuki et al. 1985; Schor et al. 1986), would provide evidence to support the hypothesis that spinal output neurons (e.g., motoneurons and sympathetic preganglionic neurons) play little integrative role in generating vestibular responses, as upstream neurons have corresponding activity. In contrast, if the responses of thoracic interneurons were distinct from those of motoneurons and sympathetic preganglionic neurons in the same segments, it would be suspected that signal integration by the output neurons is a key step in shaping their responses. The goal of the current study was to distinguish between these possibilities, and to provide a description of the characteristics of the responses of thoracic interneurons to natural and electrical stimulation of labyrinthine afferents.

Methods and materials

All procedures on animals performed in this study were approved by the University of Pittsburgh’s Institutional Animal Care and Use Committee, and conformed to the Guide for the Care and Use of Laboratory Animals (Institute for Laboratory Animal Research, 1996). Data were collected from 19 purpose-bred adult cats obtained from Liberty Research (Waverly, NY, USA). Five of the animals were employed in experiments considering the effects of electrical stimulation of vestibular afferents on the activity of thoracic interneurons, whereas the other 14 were used in studies that employed natural vertical vestibular stimulation produced by rotating the animal’s body about the roll (longitudinal) and pitch (transverse) axes using a computer-controlled hydraulic tilt table (Neurokinetics, Pittsburgh, PA, USA). Extracellular recordings of stable negative or biphasic spikes, which presumably originated from cell bodies, were made from single neurons in the T1-T6 spinal segments. Several criteria were employed to identify a cell as a putative interneuron. In most animals, floating antidromic stimulation electrodes were placed into the lateral white matter of spinal segments adjacent to the one sampled for single unit activity to determine if a neuron had ascending or descending projections (see Fig. 1B for an example); these electrodes were inserted by hand through the dorsal root entry zone to a depth of ~ 2 mm. Typically a single electrode was positioned on each side both rostral and caudal to the segment targeted for recording. Any cells with ascending or descending projections were deemed to be interneurons, despite their position in the gray matter. Another criterion employed was location: only cells demonstrated in postmortem histological reconstructions to have been clearly segregated from motoneurons (unless they had ascending or descending projections) were classified as interneurons. This benchmark excluded interneurons in close proximity to the motor pools from our sample, but assured that motoneurons were discounted. A third criterion, firing rate, was employed to ensure that sympathetic preganglionic neurons were removed from the sample. It has been established that sympathetic preganglionic neurons have very low firing frequencies even when sensory pathways that influence the cells are being activated; the modal firing rate of the sympathetic output cells is only approximately 1 spike/s (Gebber and McCall 1976; Barman and Gebber 1984), whereas pre-sympathetic interneurons have much higher discharge frequencies. Our sample of presumed interneurons had mean spontaneous firing rates of 23±2 (SEM) spikes/s; the median was 15 spikes/s and the mode was 19 spikes/s. Because the units in our sample had appreciable spontaneous firing rates, they were unlikely to be preganglionic neurons. However, this criterion also potentially excluded interneurons with low firing rates from the data sample, and it is possible that this population of cells had different responses to vestibular stimulation than units with higher spontaneous activity.

Figure 1
A: Comparison of the firing of a neuron (bottom trace) with fluctuations in blood pressure (top panels). The uppermost panel depicts a raw blood pressure recording, whereas the middle panel shows the same data after smoothing using a 0.2 s time constant. ...

Putative interneurons were also tested to determine if their firing was correlated to chest movements related to ventilation. The timing of these chest movements was ascertained from fluctuations in blood pressure recorded from the abdominal aorta, as shown in Fig. 1A.

Surgical procedures

Each animal was initially anesthetized with isoflurane vaporized in oxygen. Administration of drugs was accomplished through catheters placed in both femoral veins, and blood pressure was monitored using a transducer (Millar Instruments, Houston, TX, USA) inserted through a femoral artery into the abdominal aorta. An infrared lamp and heating pad were used to maintain rectal temperature between 36–38°C. The animal’s head was placed in a stereotaxic frame modified to pitch the head down 30°, in order to vertically align the vertical semicircular canals (Estes et al. 1975). The stereotaxic frame was mounted on a servo-controlled hydraulic tilt table. Hip pins and two vertebral clamps were used to secure the animal’s body; the vertebral clamps were placed on the dorsal processes of the vertebrae immediately rostral and caudal to the 3–4 upper thoracic spinal cord segments exposed by laminectomy. A midcollicular decerebration was performed subsequent to ligation of the carotid arteries and aspiration of the cerebral cortex overlying the rostral brainstem. In the five animals used in experiments employing electrical activation of vestibular afferents, an additional surgical procedure described previously (Yates et al. 1993) was done to prepare the vestibular nerves for bipolar electrical stimulation. The tympanic bulla was exposed on both sides using a ventrolateral approach and opened to expose the promontory. The scala vestibuli was exposed and a silver-silver chloride ball electrode insulated except at the tip was inserted in the direction of the vestibule. A second electrode was placed 1–2 mm away in the vicinity of the oval window. The electrodes were covered with warm paraffin wax and held in place using dental cement. An additional laminectomy was performed in these animals to expose the rostral portion of the C1 spinal segment, to provide for recordings of field potentials from descending vestibulospinal projections in the medial longitudinal fasciculus (MLF).

Anesthesia was stopped after the decerebration and all other surgical procedures were complete at least 1 h prior to the onset of recording. The animals were then paralyzed using a 10 mg/kg intravenous injection of gallamine triethiodide (Sigma, St. Louis, MO, USA). Paralysis was maintained using hourly supplemental injections of 5 mg/kg of gallamine. During paralysis, animals were artificially respired using a positive-pressure ventilator, and end-tidal CO2 was maintained at 3–4%. We maintained CO2 at low levels to diminish respiratory-related discharges of thoracic neurons, which might have distorted neuronal activity related to vestibular stimulation. If mechanical instability in recording conditions related to respiration was detected, a bilateral pneumothorax was generated.

At the end of the recording session, animals were killed by a 120 mg/kg intravenous sodium pentobarbital injection, and the exposed thoracic spinal cord segments were removed and fixed in 10% Formalin.

Data recording procedures

Extracellular recordings were made from thoracic neurons using epoxy-insulated tungsten microelectrodes with an impedance of 5 or 12 MΩ (A&M Systems, Sequim, WA, USA). Systematic tracking was done in ~0.2-mm steps in the medial–lateral direction and ~0.5-mm steps in the anterior–posterior direction to isolate spontaneously-active neurons. In the 14 experiments where natural vestibular stimulation was employed, combined sinusoidal pitch and roll rotations (typically at 7.5°–10° amplitude and 0.2 Hz frequency) were delivered during tracking to facilitate identifying units whose activity was modulated by whole-body tilts. In the experiments where electrical vestibular stimulation was utilized, every spontaneously-active cell encountered was tested for responses to current delivered to labyrinthine afferents.

Activity recorded by the microelectrode was amplified by a factor of 10,000 and filtered with a bandpass of 300–10,000 Hz. The output of the amplifier was fed into a window discriminator for the delineation of spikes from single units. The discriminator output was sampled at 10,000 Hz using a Micro1401 mk 2 data collection system and Spike-2 version 6 software (Cambridge Electronic Design, Cambridge, UK) running on an Intel Macintosh computer with the Microsoft Windows XP operating system and “Boot Camp” program installed. The raw output from the amplifier was also sampled at 10,000 Hz, to permit a post-hoc determination of whether spike shape remained constant throughout the recording period and unit discrimination had been adequate. Consistency of spike shape was ascertained through visual inspection of the waveforms. Two electrolytic lesions were made at predetermined depths in a subset of recording tracks by passing a 20-μA negative current for 2 min, so that locations of units could be reconstructed.

When a neuron was encountered, we determined its spontaneous activity in the absence of stimulation by sampling firing over a period of 30–60 sec. Recordings of blood pressure were made simultaneously (sampled at 100 Hz using the Cambridge Electronic Design data collection system) to permit a determination of whether the unit’s activity was synchronized to oscillations in blood pressure related to artificial ventilation (see Fig. 1A). Subsequently, we tested whether the unit could be antidromically activated by stimulation of spinal segments adjacent to the recording site, by delivering stimulus pulses that were 0.2 ms in duration and typically had maximal amplitudes of 200–500 μA. The presence of antidromic responses to spinal stimulation was confirmed using collision (see Fig. 1B for an example). Following this initial analysis, the unit’s responses to electrical or natural stimulation of vestibular afferents were determined as described below.

Recording and analysis of responses to electrical vestibular stimulation

In five animals, square-wave current pulses, 0.2 ms in duration, were employed to stimulate the vestibular nerves. During the implantation of electrodes in the inner ear, the effectiveness of vestibular nerve stimulation was determined by monitoring eye movements related to the vestibulo-ocular reflex (elicited by stimulation using a 50-shock train with a 3-msec interpulse interval), and the difference in intensity required to produce eye and facial movements was noted. The facial movements were due to stimulus spread to motor axons in the facial nerve, the closest non-target nerve to the stimulating electrodes (Gacek 1969; Wilson et al. 1977). During the recording session, the maximal stimulus intensity was limited to values lower than those shown to produce facial movements. Current intensities required to alter the activity of thoracic interneurons were expressed as multiples of the threshold for producing field potentials recorded from the MLF in rostral C1.

To determine whether a putative thoracic interneuron received vestibular inputs, a five-shock train with an interpulse interval of 3 ms at an intensity five times that required to produce MLF field potentials was employed to activate vestibular afferents. Stimulus trains were delivered at a rate of one per 1–2 s. Shorter trains were used to determine the minimal number of shocks required to produce a response. Response latencies were calculated from the last shock of the shortest train that produced a response, referred to as the ‘effective shock’. Additionally, the minimum stimulus intensity required to produce a response when using a five-shock train was determined.

Recording and analysis of responses to natural vestibular stimulation

In 14 animals, we recorded the responses of putative thoracic interneurons to stimulation of vertical semicircular canals and otolith organs, which was produced by tilting the animal about the pitch and roll axes. Our procedures for performing vertical vestibular simulation have been described in detail previously (Jian et al. 2002). We first determined the plane of tilt that produced maximal modulation of the unit’s firing rate (response vector orientation). Response vector orientation was calculated from responses to the “wobble” stimulus, a constant-amplitude tilt whose direction moves around the animal at constant speed (Schor et al. 1984). The direction of the response vector orientation lies midway between the maximal response directions to clockwise (CW) and counterclockwise (CCW) wobble stimulation, because the phase differences between stimulus and response are reversed during the two directions of rotation (Schor et al. 1984). Thus, by consideration of both responses, these phase differences could be accounted for. Wobble stimulation was typically delivered at 0.2 Hz, and sometimes at lower and higher frequencies as well. Subsequently, the response vector orientation was confirmed by comparing the gain of responses to tilts in a variety of fixed vertical planes, typically delivered at 0.2 Hz and at an amplitude of 7.5°. These tilts always included the roll and pitch planes. After a unit’s response vector orientation was established, planar tilts at or near this orientation were used to study the dynamics of the vestibular response (i.e., response gain and phase across stimulus frequencies). Response dynamics were routinely determined over the frequency range of 0.1–1 Hz; for some cells rotations at 0.02, 0.05 Hz, and/or 2 Hz were also delivered. The amplitudes of these stimuli were usually 2.5–7.5° at frequencies ≥0.5 Hz, 5–10° at 0.2 Hz, and 7.5–15° at lower frequencies.

Neural activity recorded during rotations in vertical planes was binned (1,000 bins/cycle) and averaged over the sinusoidal stimulus period. When a unit’s spontaneous activity was rhythmic (e.g., synchronized with ventilation), sufficient sweeps were collected to average-out the oscillations in firing that were unrelated to the stimulus. Sine waves were fitted to responses with the use of a least-squares minimization technique (Schor et al. 1984); an initial analysis was performed using the Spike-2 software as data collection was occurring, and a post-hoc final analysis was subsequently executed using Matlab (The MathWorks, Natick, MA, USA). The response sinusoid was characterized by two parameters: phase shift from the stimulus sinusoid (subsequently referred to as phase) and amplitude relative to the stimulus sinusoid (subsequently referred to as gain). Responses were considered significant if the signal-to-noise ratio (see (Schor et al. 1984) for method of calculation) was ≥ 0.5 and only the first harmonic was prominent.

Reconstruction of recording sites and statistical analysis of data

The thoracic spinal segments containing recording sites were cut into 50 μm transverse sections that were collected serially and stained using thionine. Recording sites were reconstructed using camera lucida relative to electrolytic lesion placement, positions of electrode tracks, and microelectrode depth. The locations of the motor pools were noted in camera lucida drawings, and units were considered to be thoracic interneurons only if they were segregated from motoneurons or could be antidromically activated by stimulation in an adjacent cord segment.

Statistical analyses were performed using Prism 5 software (GraphPad Software, San Diego, CA, USA). Pooled data are presented as means ± one standard error.

Results

Responses of thoracic interneurons to electrical stimulation of vestibular afferents

A mean current intensity of 52±9 μA (median of 45 μA) delivered to the vestibular nerve was needed to elicit field potentials recordable from the MLF, whereas an intensity of 435±44 μA (median of 405 μA) was required to produce stimulus spread to the facial nerve. As such, the maximal currents used to test whether thoracic interneurons received labyrinthine inputs (5 times the MLF field threshold) were well below those that activated fibers in the closest non-target nerve to the stimulation site.

A total of 102 putative thoracic interneurons were examined for responses to vestibular nerve stimulation: 35 were located in the dorsal horn (Rexed’s laminae I–V), 64 were positioned in the intermediate zone (Rexed’s laminae VI–VIII and X), and 3 were isolated in the vicinity of motoneurons but could be antidromically activated by stimulation of the white matter caudal to the recording site. Out of this population, 21/35 dorsal horn neurons, 50/64 intermediate zone cells, and all three putative interneurons located in the motor pool responded to activation of labyrinthine afferents. Of the 74 units that received vestibular inputs, the firing of 62 was modulated by stimulation of both labyrinths, 9 were affected only by contralateral stimulation, and 3 responded only to ipsilateral stimulation. Typically, the responses to stimulation of both sides were similar. The largest proportion of cells that received vestibular inputs (39/74) were predominantly excited, 24 were initially excited and then inhibited, 4 were inhibited, and 7 displayed a response consisting of initial inhibition followed by excitation. Examples of responses to vestibular nerve stimulation are shown in Fig. 2, panels A and B.

Figure 2
Responses of thoracic neurons to electrical stimulation of the vestibular nerve. A: Responses of a neuron that exhibited short-latency excitation followed by inhibition during vestibular stimulation. Trace A1 is a poststimulus histogram showing the response ...

Fig. 2C shows for each of the units the latency of the earliest response elicited by stimulation of either the ipsilateral or contralateral labyrinth. The latency was calculated from the effective shock, or last shock of the shortest train that evoked changes in neuronal activity; four cells were excluded because they were lost before the effective shock was determined. The majority of cells that received vestibular inputs (51/70) responded to a single shock delivered to the labyrinth, whereas 10 cells required two shocks, 7 needed three shocks, while longer trains were necessary for the others. The response latency for approximately half of the neurons (32/70) was <5.5 msec from the effective shock; these cells were deemed to have short-latency responses. The locations of these neurons in the spinal gray matter, as well as the positions of neurons with medium-latency (<10.5 msec) and long-latency (≥10.5 msec) responses to vestibular stimulation are shown in Fig. 3B. The mean response latency was 9.4±1.3 msec from the effective shock, although the median response latency was much shorter: 6.5 msec.

Figure 3
Locations of putative interneurons plotted on transverse sections of the upper thoracic spinal gray matter; the segment depicted in each diagram is indicated. A: Locations of neurons whose responses to natural vestibular stimulation were characterized. ...

A small fraction of the units (10/102) had activity correlated with blood pressure fluctuations related to ventilation. The firing of 7 of these cells was affected by vestibular nerve stimulation; their relative response latencies are reflected in Fig. 3B. In addition, 12 neurons could be antidromically activated by stimulation in an adjacent cord segment; 2 of these cells were located in the dorsal horn, 7 in the intermediate zone, and 3 in the motor pools. Most (9/12) had caudally-projecting axons, although 3 projected rostrally; the mean antidromic threshold for the neurons was 140±40 μA, and the mean conduction velocity was 22±3 m/sec. The firing of a large majority of the neurons (11/12) with ascending or descending projections was altered by electrical stimulation of the vestibular nerve; the mean response latency was 10±2 msec.

Responses of thoracic interneurons to natural stimulation of vestibular afferents

We characterized the responses of 66 putative thoracic interneurons to rotations in vertical planes. The locations of these neurons are shown in Fig. 3A; 12 were positioned in the dorsal horn, 53 in the intermediate zone, and 1 cell found in the motor pool was deemed to be an interneuron because it could be antidromically activated by stimulation of the white matter of a caudal spinal segment. Examples of the responses of two neurons to vertical rotations are shown in columns A and B of Fig. 4. Panels 1 and 2 illustrate neuronal activity elicited by wobble rotations, whereas panels 3–5 show responses to different frequencies of roll stimuli.

Figure 4
Responses of two putative thoracic interneurons to sinusoidal rotations in vertical planes; gray traces show average neuronal firing rate (in Hz per degree of rotation), whereas black traces indicate the position of the tilt table. Column A illustrates ...

Bode plots illustrating the dynamic properties of responses of thoracic interneurons to rotations in fixed planes near the response vector orientation are shown in Fig. 5. Both response gain and phase were plotted with respect to stimulus position, such that a response whose phase leads stimulus position by 90° is synchronous with stimulus velocity. The large majority of neurons (48/66) had response phases that remained within 45° of stimulus position at all frequencies tested (see Fig. 5A). Most of these cells (35/48) also had response gains that remained relatively consistent across stimulus frequencies, increasing < 3-fold per stimulus decade. Only about 10% (5/48) of the thoracic interneurons with response phases synchronous with stimulus position had response gains that increased > 7-fold per stimulus decade. As such, the changes in firing of a majority of thoracic interneurons during vertical vestibular stimulation suggested that they received inputs from graviceptors. However, a few units had alternate response dynamics. Three cells had response phases near stimulus velocity (90°) at all frequencies tested, and seven neurons had responses that were synchronous with stimulus position when low rotational frequencies were employed that advanced to near stimulus velocity when high-frequency tilts were delivered. The response dynamics of these units are illustrated in Fig. 5B. The responses of nine cells developed a phase lag as stimulus frequency increased: four lagged the velocity of the tilt (90°) whereas five lagged stimulus position (0°), as indicated in Fig. 5C. The response gains of these cells remained relatively consistent across stimulus frequencies, increasing no more than 5-fold per stimulus decade.

Figure 5
Bode plots illustrating the dynamic properties of responses of putative thoracic interneurons to rotations at multiple frequencies. Response gain and phase are plotted relative to stimulus position. A: Bode plots for neurons whose response phases remained ...

The response vector orientations of thoracic interneurons are shown in Fig. 6. Fig. 6A depicts data for cells whose response phases were near stimulus position at all frequencies of whole-body rotation. The directions of tilt that optimally activated these neurons were highly varied, with 18 being nearest (within 22.5°) to a canal plane, 14 being nearest to pitch, and 16 being nearest to roll. Although a few cells activated by roll tilts had particularly large responses to rotations, the median response gains to wobble stimuli were similar for units that were maximally affected by rotations near the roll, pitch, and canal planes. For example, the median response gain to the highest-frequency wobble stimulus delivered was 1.2 spikes/s/° for cells best activated by roll-plane stimuli, 1.1 spikes/s/° for neurons that responded best to pitch, and 1.0 spikes/s/° for units maximally affected by canal-plane rotations. In addition, most (36/48) of the graviceptive neurons were excited by ipsilateral ear down rotations, whereas 12 were excited by contralateral ear down tilts. Neurons with other than graviceptive responses to vertical vestibular stimulation similarly had a wide diversity of response vector orientations, but were more commonly excited by contralateral than ipsilateral tilts (see Fig. 6B–C). For the large majority of cells (60/66), the gains of responses to the two directions (CW and CCW) of wobble rotation varied no more than 2:1. Neurons that receive vestibular inputs with different spatial and temporal properties, and thus can be classified as having spatiotemporal convergence (STC) behavior, typically have responses to CW and CCW wobble rotations of greatly different magnitude (at least > 2:1) because the misaligned inputs to the cell add during one direction of rotation but subtract when rotations are delivered in the opposite direction (Wilson et al. 1986; Kasper et al. 1988; Schor and Angelaki 1992). As such, it appears that few thoracic interneurons have STC responses to rotations in vertical planes.

Figure 6
Polar plots showing the response vector orientations and gains for thoracic interneurons, determined using wobble stimuli that were usually delivered at 0.2 Hz. Numbers along the radius of each plot indicate gain (spikes/s/°). A: Data for neurons ...

Less than a quarter of the neurons that responded to natural vestibular stimulation (15/66) had activity that was synchronized with ventilation; in addition, putative thoracic interneurons included in our sample did not exhibit spontaneous oscillations in activity other than those related to breathing. The responses of this subset of units did not appear to differ from the population as a whole. For example, the response vector orientations of these cells were just as varied as those for neurons that lacked ventilator-related activity: 6 were best activated by rotations near the plane of a semicircular canal, 6 responded best to pitch oscillations, and 3 were best stimulated by roll tilts. In addition, 6 of the thoracic interneurons that responded to vertical vestibular stimulation could be antidromically activated by stimulation of the white matter of an adjacent spinal segment. All 6 of the antidromically-activated units were located in the intermediate zone or motor pool; 4 had rostrally-projecting axons and two projected caudally. Their conduction velocities ranged from 10–30 m/sec (median of 18 m/sec), and the antidromic thresholds ranged from 50–360 μA (median of 185 μA). All of the interneurons with ascending or descending projections had response vector orientations near the plane of a semicircular canal, and most (4/6) had dynamic responses to tilts similar to graviceptors.

Discussion

The present findings suggest that a large fraction of thoracic interneurons receive labyrinthine inputs, and that about half of these cells respond to vestibular nerve stimulation at latencies < 5.5 msec. Prior studies have shown that vestibular nucleus neurons that receive monosynaptic inputs from VIIIth nerve afferents respond to stimulation of the labyrinth at latencies < 1.4 msec (Zhang et al. 2001; Kushiro et al. 2008), whereas an average of 0.6–0.7 msec is required to elicit monosynaptic EPSPs in upper thoracic neurons following stimulation of descending projections at the level of the brainstem (Wilson et al. 1970a; Wilson et al. 1970b). Even considering that an additional ~0.5 msec would be needed for the excited neurons to generate an action potential, only cells with response latencies < 3 msec from the effective shock could safely be classified as receiving disynaptic inputs from the inner ear. As such, although many thoracic interneurons receive relatively short-latency vestibular signals, these are mainly relayed through trisynaptic and longer pathways.

The spatial and temporal properties of responses of thoracic interneurons to vertical vestibular stimulation were distinct from those of interneurons located in the C7 segment of the cervical enlargement, which were established using a hydraulic tilt table very similar to the one employed in the present study (Schor et al. 1986). The majority of cervical enlargement interneurons (15/26) were classified as having “velocity responses,” or phases that were near stimulus velocity when rotational frequencies ≥ 0.2 Hz were delivered (a combination of the “phase near 90°” and “advancing phase” categories utilized in the present study)(Schor et al. 1986), whereas only 9/66 cells in the upper thoracic spinal cord had such response properties. As such, velocity responses are less prevalent amongst thoracic interneurons than C7 interneurons (p<0.001, Fisher’s exact test). Furthermore, 69% of cervical enlargement interneurons had response vector orientations near the roll plane and the remaining 31% were best activated by rotations near a canal plane (Schor et al. 1986). In contrast, only 33% of upper thoracic interneurons were best activated by roll rotations, whereas 41% and 26% of the cells had response vector orientations nearest a canal plane or the pitch axis, respectively. A χ2 analysis revealed that the distribution of response vector orientations differed between the two populations of cells (p<0.001). Neurons in the lumbar cord have response dynamics that are much more similar to neurons in the cervical cord than to thoracic interneurons. For example, out of 46 neurons studied in the L3-L4 segments in decerebrate cats, 39 had response vector orientations closer to the roll than to the pitch axis (Suzuki et al. 1985); this distribution of response vector orientations is significantly different than for thoracic neurons (p<0.001, Fisher’s exact test). It thus appears that the vestibular signals sent to the upper thoracic spinal cord are distinct from those relayed to segments that control limb movements; in particular, the thoracic cord appears to receive fewer inputs related to stimulus velocity, but more signals reflecting changes in body orientation around the transverse axis.

The activity of a majority of cervical interneurons during vertical vestibular stimulation is similar to that of forelimb muscles; both are stimulated by tilts about the longitudinal axis and have graviceptive responses to low rotational frequencies but velocity responses at high frequencies (Wilson et al. 1986). In contrast, muscles innervated by thoracic motoneurons whose activities have been examined during vertical vestibular stimulation as well as thoracic sympathetic outflow are activated by tilts about the transverse axis and have graviceptive responses across the spectrum of frequencies tested (Yates and Miller 1994; Rossiter et al. 1996; Brophy et al. 1997). As such, the dynamic properties of responses of most thoracic interneurons to rotations in vertical planes were similar to those of thoracic output. However, the matching of the spatial properties of the responses was not precise. In particular, the response vector orientations of approximately half of the upper thoracic interneurons (34/66) were closer to the roll plane than the pitch plane. The simple addition of signals from these interneurons would not generate a response aligned with the transverse axis as is observed in thoracic output neurons (Yates and Miller 1994; Rossiter et al. 1996; Brophy et al. 1997). It thus appears that the responses of thoracic sympathetic preganglionic neurons and motoneurons to vestibular stimulation are not a simple reflection of the activity already manifested in the firing of local interneurons, and that many output neurons perform an integration of signals with spatial properties distinct from those manifested in their discharges. Perhaps the signal processing required by thoracic motoneurons and sympathetic neurons is more extensive than that done by forelimb and hindlimb motoneurons.

A limitation of this study is that we could not ascertain the targets of the interneurons whose activity was recorded. Prior studies have shown that respiratory interneurons in the thoracic spinal cord are located in the intermediate zone dorsomedial to the motoneurons, and outnumber the motoneurons by a 10:1 ratio (Kirkwood et al. 1988; Kirkwood et al. 1993; Schmid et al. 1993). Our sample included neurons in this area, although only a few displayed rhythmic discharges related to respiration (it would be presumed that the respiratory rhythm in these experiments was coupled with mechanical ventilation, particularly since large air volumes were employed to maintain end-tidal CO2 ≤ 4% in most animals (Petrillo and Glass 1984)). However, the low CO2 also likely diminished the magnitude of the central respiratory rhythm, such that respiratory interneurons were difficult to recognize. Presympathetic interneurons have been identified in the vicinity of the intermediolateral cell column, the dorsal horn, and the intermediate zone (Barman and Gebber 1984; Tang et al. 2003; Tang et al. 2004; Schramm 2006); vestibular-activated cells were located in all of these regions. The contribution of interneurons to regulating back muscle activity has not been ascertained, but presumably such premotor neurons are present in the thoracic spinal cord. However, the fact that vestibular inputs to thoracic interneurons were so prevalent (e.g., 74/102 cells responded to electrical stimulation of the vestibular nerve) suggests that all of these subgroups of units, and likely others, receive inputs from the inner ear.

In summary, the present data show that vestibular inputs to upper thoracic spinal interneurons are prevalent, and distinct from those to other cord segments where neuronal responses to vertical vestibular stimulation have been examined. In particular, cells in the thoracic cord appear to receive fewer inputs related to stimulus velocity, but more signals reflecting changes in body orientation around the transverse axis, than neurons in the cervical and lumbar enlargements. As such, the dynamic properties of responses of thoracic interneurons to vertical rotations are more closely aligned than those of other spinal neurons to the responses of thoracic motoneurons and sympathetic preganglionic neurons, which have graviceptive responses to rotations in the pitch plane. Nonetheless, the response vector orientations of the thoracic interneurons were heterogeneous, with only half being better activated by pitch than roll rotations. As such, the activity of the thoracic output cells, which is modulated by pitch rotations, does not reflect a simple addition of the diverse responses of the local interneurons to vestibular stimulation. Rather, a more complex integrative process must occur at the level of the output cells.

Acknowledgments

The authors thank Lucy Cotter, Allison Waggoner, and Michael Bonadio for technical assistance. Funding was provided by Grant R01-DC03732 from the National Institutes of Health (USA). Core support was provided by NIH grant P30-DC05205.

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