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J Clin Microbiol. 1988 December; 26(12): 2489–2494.
PMCID: PMC266931

Monoclonal antibodies to Mokola virus for identification of rabies and rabies-related viruses.


Rabies and rabies-related virus strains were studied by using a panel of monoclonal antibodies directed against either nucleocapsid proteins or cell surface antigens of Mokola virus (Mok-3). Each strain was used in parallel to infect cultured cells and mice. Then, the patterns of reactivity of the different monoclonal antibodies were determined by the immunofluorescent-antibody staining procedure. On cells, the monoclonal antibodies differentiated fixed rabies virus strains (serotype 1) from rabies-related virus strains. The seven fixed strains (CVS, PV4, PM, Flury LEP and HEP, ERA, and SAD) reacted identically. The previous serotype groupings (serotype 2, Lagos-bat virus; serotype 3, Mokola virus; serotype 4, Duvenhage virus) established with anti-rabies monoclonal antibodies were confirmed, except for that of Lagos-bat Kindia, which appeared to be related to the African subtype of the Duvenhage serotype (Duv-2). Within the Mokola (Mok-1, -2, -3, and -5 and Umhlanga) and the Lagos-bat (Lag-1 and -2, Zimbabwe, Pinetown, and Dakar) serotypes, each strain appeared to be distinct. The African subtype of the Duvenhage serotype reacted differently from the European subtype. Within the Duvenhage serotype, subtypes Duv-4, -5, and -6 and Denmark reacted identically, while subtypes Duv-1, -2, and -3 and German Democratic Republic appeared to be distinct. The monoclonal antibodies specific for the cell surface antigens were also used in neutralization tests with all the strains. Two of them neutralized the infectivity of Mokola virus.

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Selected References

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  • BOULGER LR, PORTERFIELD JS. Isolation of a virus from Nigerian fruit bats. Trans R Soc Trop Med Hyg. 1958 Sep;52(5):421–424. [PubMed]
  • Bussereau F, Perrin P. Cellular response to rabies virus infection. Comp Immunol Microbiol Infect Dis. 1982;5(1-3):49–59. [PubMed]
  • Crick J, Tignor GH, Moreno K. A new isolate of Lagos bat virus from the Republic of South Africa. Trans R Soc Trop Med Hyg. 1982;76(2):211–213. [PubMed]
  • Fabiyi A, Osunkoya BO, Kemp GE. Pathogenicity of Mokola, a rabies-related virus, in baby mice. Mt Sinai J Med. 1974 Jan-Feb;41(1):120–124. [PubMed]
  • Familusi JB, Osunkoya BO, Moore DL, Kemp GE, Fabiyi A. A fatal human infection with Mokola virus. Am J Trop Med Hyg. 1972 Nov;21(6):959–963. [PubMed]
  • Foggin CM. Atypical rabies virus in cats and a dog in Zimbabwe. Vet Rec. 1982 Apr 3;110(14):338–338. [PubMed]
  • Lafon M, Herzog M, Sureau P. Human rabies vaccines induce neutralising antibodies against the European bat rabies virus (Duvenhage) Lancet. 1986 Aug 30;2(8505):515–515. [PubMed]
  • Le Gonidec G, Rickenbach A, Robin Y, Heme G. Isolement d'une souche de virus Mokola au Cameroun. Ann Microbiol (Paris) 1978 Feb-Mar;129(2):245–249. [PubMed]
  • Libeau G, Lafon M, Rollin PE. Etude de la spécificité d'anticorps monoclonaux obtenus avec la souche de virus de rage Pasteur PV. Rev Elev Med Vet Pays Trop. 1984;37(4):383–394. [PubMed]
  • Meredith CD, Prossouw AP, Koch H van P. An unusual case of human rabies thought to be of chiropteran origin. S Afr Med J. 1971 Jul 17;45(28):767–769. [PubMed]
  • Meredith CD, Standing E. Lagos bat virus in South Africa. Lancet. 1981 Apr 11;1(8224):832–833. [PubMed]
  • Murphy FA, Bauer SP, Harrison AK, Winn WC., Jr Comparative pathogenesis of rabies and rabies-like viruses. Viral infection and transit from inoculation site to the central nervous system. Lab Invest. 1973 Mar;28(3):361–376. [PubMed]
  • Rollin PE, Sureau P. Monoclonal antibodies as a tool for rabies epidemiological studies. Dev Biol Stand. 1984;57:193–197. [PubMed]
  • Shope RE, Murphy FA, Harrison AK, Causey OR, Kemp GE, Simpson DI, Moore DL. Two African viruses serologically and morphologically related to rabies virus. J Virol. 1970 Nov;6(5):690–692. [PMC free article] [PubMed]
  • Smith JS, Reid-Sanden FL, Roumillat LF, Trimarchi C, Clark K, Baer GM, Winkler WG. Demonstration of antigenic variation among rabies virus isolates by using monoclonal antibodies to nucleocapsid proteins. J Clin Microbiol. 1986 Oct;24(4):573–580. [PMC free article] [PubMed]
  • Sureau P, Germain M, Herve JP, Geoffroy B, Cornet JP, Heme G, Robin Y. Isolement du virus Lagos-bat en Empire Centrafricain. Bull Soc Pathol Exot Filiales. 1977 Sep-Oct;70(5):467–470. [PubMed]
  • Sureau P, Rollin P, Lafon M. Characterization of rabies virus strains used for vaccine production by means of monoclonal antibodies. Dev Biol Stand. 1984;57:227–231. [PubMed]
  • Sureau P, Rollin P, Wiktor TJ. Epidemiologic analysis of antigenic variations of street rabies virus: detection by monoclonal antibodies. Am J Epidemiol. 1983 May;117(5):605–609. [PubMed]
  • Tignor GH, Shope RE. Vaccination and challenge of mice with viruses of the rabies serogroup. J Infect Dis. 1972 Mar;125(3):322–324. [PubMed]
  • Vincent J, Bussereau F, Sureau P. Immunological relationships between rabies virus and rabies-related viruses studied with monoclonal antibodies to Mokola virus. Ann Inst Pasteur Virol. 1988 Apr-Jun;139(2):157–173. [PubMed]
  • Wiktor TJ, Macfarlan RI, Foggin CM, Koprowski H. Antigenic analysis of rabies and Mokola virus from Zimbabwe using monoclonal antibodies. Dev Biol Stand. 1984;57:199–211. [PubMed]

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