PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
 
BMC Genomics. 2009; 10: 133.
Published online Mar 27, 2009. doi:  10.1186/1471-2164-10-133
PMCID: PMC2667192
Genomic regions with distinct genomic distance conservation in vertebrate genomes
Hong Sun,1,2,3,5 Geir Skogerbø,4 Xiaohui Zheng,6 Wei Liu,corresponding author2 and Yixue Licorresponding author1,3
1Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, PR China
2Biological Technologies, Wyeth Research, Cambridge, Massachusetts, USA
3Shanghai Center for Bioinformation Technology, Shanghai, PR China
4Bioinformatics Laboratory and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, PR China
5Zhongxin Biotechnology Shanghai Co Ltd, Shanghai, PR China
6Hetian Peoples' Hospital, Xinjiang, PR China
corresponding authorCorresponding author.
Hong Sun: sunhong/at/scbit.org; Geir Skogerbø: zgb/at/ict.ac.cn; Xiaohui Zheng: mrxq123/at/hotmail.com; Wei Liu: wliu/at/wyeth.com; Yixue Li: yxli/at/sibs.ac.cn
Received October 21, 2008; Accepted March 27, 2009.
Abstract
Background
A number of vertebrate highly conserved elements (HCEs) have been detected and their genomic interval distances have been reported to be more conserved than protein coding genes among mammalian genomes. A characteristic of the human – non-mammalian comparisons is a bimodal distribution of relative distance difference of conserved consecutive HCE pairs; and it is difficult to attribute such profile to a random assortment. We therefore undertook an analysis of the human genomic regions confined by consecutive HCE pairs common to eight genomes (human, mouse, rat, chicken, frog, zebrafish, tetradon and fugu).
Results
Among HCE pairs, we found that some consistently preserve highly conserved interval distance among genomes while others have relatively low distance conservation. Using a partition method, we detected two groups of inter-HCE regions (IHRs) with distinct distance conservation pattern in vertebrate genomes: IHR1s that are bordered by HCE pairs with relative small distance variation, and IHR2s with larger distance difference values. Compared to random background, annotated repeat sequences are significantly less frequent in IHR1s than IHR2s, which reflects a correlation between repeat sequences and the length expansion of IHRs. Both groups of IHRs are unexpectedly enriched in human indel (i.e. insertion and deletion) polymorphism-variations than random background. The correlation between the percentage of conserved sequence and human IHR length was stronger for IHR1 than IHR2. Both groups of IHRs are significantly enriched for CpG islands.
Conclusion
The data suggest that subsets of HCE pairs may undergo different evolutionary paths in light of their genomic distance conservation, and that sets of genomic regions pertain to HCEs, as well as the region in which HCEs reside, should be treated as integrated domains.
Articles from BMC Genomics are provided here courtesy of
BioMed Central