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Philos Trans R Soc Lond B Biol Sci. 2009 April 12; 364(1519): 1021–1032.
Published online 2008 December 5. doi:  10.1098/rstb.2008.0234
PMCID: PMC2666080

Determining the function of zebrafish epithalamic asymmetry

Abstract

As in many fishes, amphibians and reptiles, the epithalamus of the zebrafish, Danio rerio, develops with pronounced left–right (L–R) asymmetry. For example, in more than 95 per cent of zebrafish larvae, the parapineal, an accessory to the pineal organ, forms on the left side of the brain and the adjacent left habenular nucleus is larger than the right. Disruption of Nodal signalling affects this bias, producing equal numbers of larvae with the parapineal on the left or the right side and corresponding habenular reversals. Pre-selection of live larvae using fluorescent transgenic reporters provides a useful substrate for studying the effects of neuroanatomical asymmetry on behaviour. Previous studies had suggested that epithalamic directionality is correlated with lateralized behaviours such as L–R eye preference. We find that the randomization of epithalamic asymmetry, through perturbation of the nodal-related gene southpaw, does not alter a variety of motor behaviours, including responses to lateralized stimuli. However, we discovered significant deficits in swimming initiation and in the total distance navigated by larvae with parapineal reversals. We discuss these findings with respect to previous studies and recent work linking the habenular region with control of the motivation/reward pathway of the vertebrate brain.

Keywords: habenula, brain asymmetry, behaviour

1. Introduction

The functional significance of brain laterality has been a long-debated topic in cognitive neuroscience. Theories abound as to the advantages of the left–right (L–R) specialization of the nervous system and as to why directional biases in neuroanatomy and behaviour are found throughout the animal kingdom (Vallortigara & Rogers 2005). For example, light-induced neuroanatomical asymmetry in the visual system of developing birds correlates with some enhanced visual behaviours in adulthood (Güntürkün et al. 2000; Rogers 2008), and preferential eye use has been argued to mediate shoaling behaviour in social fish species (Bisazza et al. 2000).

Fishes are a valuable system for examining functional lateralization at the individual and population level (Bisazza et al. 1998). Because the eyes are positioned laterally on the head and each is exposed to a different visual landscape, left or right eye use upon viewing familiar or novel objects, or when self-viewing (‘mirror test’) provides a simple assay to detect biases (Facchin et al. 1999; Sovrano et al. 1999; De Santi et al. 2001; Sovrano et al. 2001). Systematic preferences in eye use are proposed to be a behavioural manifestation of specialization of the two sides of the brain in processing incoming visual information, since each eye predominately projects to the contralateral side of the brain (Vallortigara 2000). Turning to avoid barriers or to navigate complex environments, prey capture and aggressive behaviours also have been found to have a preferred directional component in some fish species (e.g. Heuts 1999; Bisazza et al. 2000, 2001; Bisazza & de Santi 2003; Reddon & Hurd 2008 and refer to Vallortigara & Bisazza 2002).

The zebrafish, Danio rerio, has obvious benefits in exploring behavioural laterality, as a well-studied developmental model amenable to genetic manipulations. Functional lateralization in this species has been previously documented for a number of behavioural tests both in adults (Miklósi et al. 1997, 2001; Heuts 1999; Miklósi & Andrew 1999) and in young fry (Watkins et al. 2004; Barth et al. 2005; Sovrano & Andrew 2006).

Adult zebrafish show a right eye preference when first exposed to new objects or complex scenes that require immediate monitoring and response (Miklósi et al. 2001; Miklósi & Andrew 2006). However, the left eye is preferentially used on subsequent trials, for visual inspection of familiar stimuli or those with moderate novelty and, presumably, comparisons with the memory of similar stimuli. Thus, left eye viewing appears to be better equipped for comprehensive assessment of familiarity, while the right eye system has been proposed to be more resistant to distraction and to mediate decision-making responses (Miklósi et al. 1997; Miklósi & Andrew 2006). Adults also tend to use the right eye when approaching an object to bite; however, no bias in eye use is found when a familiar object is investigated and not bitten (Miklósi & Andrew 1999). When faced with a barrier blocking access to a perceived predator, adult zebrafish show a detour response that is biased for left eye inspection and turning to the right (Bisazza et al. 2000).

Larval zebrafish as young as 8 days post-fertilization (dpf) appear to exhibit behavioural biases. Watkins et al. (2004) described biases in the directionality of turning, which were correlated with changes in light intensity that an 8-day-old larva experienced while navigating through a multicompartment swimway. They also found preferential left eye inspection and less avoidance behaviour in larvae exposed to a dark stripe that had previously been presented in the left visual field. Their findings were consistent with the left eye bias described for adult zebrafish in assessing stimuli with respect to prior experiences (Miklósi et al. 1997). Sovrano & Andrew (2006) modified the mirror test to study the development of visual lateralization in zebrafish larvae and also found a preference for left eye viewing. However, left eye bias was strain, age and distance dependent and was sustained for varying periods within the testing window. A more recent study (Andrew et al. in press) also suggests that, as in developing chicks (refer to Rogers 2008), early exposure to light may influence bias in L–R eye use.

2. The zebrafish as a model of epithalamic l–r asymmetry

Recently, it has become possible to tackle the problem of how brain asymmetry arises developmentally using molecular genetic approaches afforded by the zebrafish model. Although there remains some controversy about the nature of the initial symmetry-breaking event in the early embryo, the ciliated Kupffer's vesicle present in the caudal midline at somitogenesis (Bisgrove et al. 2005; Essner et al. 2005) and Wnt signalling (Carl et al. 2007; Inbal et al. 2007) have been implicated in the determination of L–R differences. Components of the Nodal signalling pathway involved in specifying the L–R axis across vertebrates also show a conserved function in the establishment of zebrafish visceral asymmetry (refer to Liang & Rubinstein 2003; Schier 2003). However, only in fishes have Nodal-related TGF-β family members been shown to influence L–R determination in the brain, specifically in the epithalamic region of the dorsal diencephalon (Concha et al. 2000; Liang et al. 2000). Loss of Nodal-related signals (cyclops/nodal-related 2 or southpaw/nodal-related 3) does not disrupt L–R asymmetry, but rather results in a randomization in directional asymmetry across the population. For example, more than 95 per cent of all wild-type zebrafish embryos form a parapineal organ on the left side of the brain (Concha et al. 2000; Gamse et al. 2002). The parapineal is closely associated with the pineal organ and arises from cells in a shared pineal complex anlage (Concha et al. 2003; Snelson et al. 2008). In approximately 50 per cent of embryos with Nodal signalling blocked or that lack southpaw (spaw) function, the parapineal develops to the left of the pineal, while the other 50 per cent form the parapineal on the right.

While this might seem like a minor disruption, the position of the parapineal has striking consequences on the development of the epithalamic region flanking the pineal complex, the bilateral habenular nuclei, and their connectivity with a shared midbrain target. In the vast majority of larvae, the left habenula is in close apposition to the parapineal and is larger, exhibits more dense neuropil and a different gene expression profile than the right habenula (Concha et al. 2003; Gamse et al. 2003, 2005; Kuan et al. 2007a,b). L–R patterns of gene expression appear to correlate with differences in subnuclear organization and proliferation of habenular neurons (Gamse et al. 2003; Aizawa et al. 2007). The right habenula may be a default state because, when the parapineal is destroyed, the left habenular nucleus develops with properties more similar to the right habenula (Concha et al. 2003; Gamse et al. 2003). However, an exception is that distinct left and right neuronal morphologies appear to still be maintained (Bianco et al. 2008).

Neurons from the left habenula normally project their axons to dorsal and ventral regions of the interpeduncular nucleus (IPN) in the ventral midbrain, whereas projections from the right habenular neurons are confined ventrally (Gamse et al. 2005). Expression of the gene encoding the axon guidance receptor Neuropilin-1 (Nrp1) is restricted to the left habenula, which most probably accounts for the L–R difference in target connectivity (Kuan et al. 2007a,b). Morpholino-mediated disruption of Nrp1 or parapineal ablation leads to a similar outcome, with both left and right habenular efferents primarily innervating the ventral target. Larvae with the parapineal on the right side of the brain not only show a L–R reversal in habenular identity as assessed by differences in size, amount of dense neuropil and gene expression (including right habenular expression of nrp), but they also exhibit a corresponding reversal in the IPN innervation pattern (Gamse et al. 2005; Kuan et al. 2007a,b). Because neither the distinct functions of the dorsal and ventral IPN nor their post-synaptic partners have yet been determined in zebrafish, it is unknown what effect parapineal and, hence, habenular L–R reversal would have on neural pathways influenced by the habenular-IPN connection.

Mutations in a variety of developmentally important genes disrupt directional asymmetry in zebrafish embryos, and L–R randomization in mutants can uncouple visceral and brain asymmetries (Sampath et al. 1998; Essner et al. 2000). A zebrafish line, frequent-situs-inversus (fsi), that has a tendency to produce a higher than usual frequency of larvae with concordant heart, gut, pancreas and parapineal L–R reversals has also been described (Barth et al. 2005). This trait does not segregate as a simple single-gene mutation, but intercrosses within the fsi line variably increase the rate of situs inversus from 5 to 25 per cent in a single clutch. Analyses of fsi individuals with L–R reversed epithalamic neuroanatomy indicated a corresponding reversal in the directionality of some lateralized behaviours (Barth et al. 2005). The ability to alter the L–R orientation of the brain in a predictable manner by genetic manipulations is a valuable feature of the zebrafish system for studies on the behavioural consequences of an asymmetric nervous system.

Using an antisense morpholino (MO) against the spaw gene (Long et al. 2003) injected into one-cell stage embryos, we can reliably generate four distinct classes of zebrafish larvae: those with the typical pattern of left parapineal and right pancreas (designated LppRpa) that is found in more than 95 per cent of wild-type populations; those showing situs inversus or reversal of this pattern (designated RppLpa); and two discordant classes with a right parapineal and right pancreas (RppRpa) or a left parapineal and left pancreas (LppLpa) (Gamse et al. 2005). Following this experimental manipulation, the four classes are not found in equal frequencies (figure 1e); however, a significantly greater number of larvae show reversed epithalamic and visceral asymmetry compared with wild-type strains. The MO is introduced into doubly transgenic progeny from matings between Tg(foxd3:GFP)fkg17 (Gilmour et al. 2002) and Tg(ela3l:GFP)gz2;Tg(fabp10:dsRed)gz4 (Dong et al. 2007) adults, in which the pineal complex and pancreas, and the liver, are labelled with green fluorescent protein (GFP) and red fluorescent protein (RFP), respectively (figure 1ad). The resultant larvae can be unambiguously sorted at 3 dpf on the basis of the position of the GFP+ parapineal to the left or right of the pineal organ, and at 5 dpf for the location of the GFP+ pancreas on the left or the right side of the body (figure 1e). This approach allows larvae (and adults) to be maintained in four discrete anatomical classes and ensures the availability of large numbers for behavioural analyses. The LppRpa group, bearing the configuration of the majority of wild-type or transgenic larvae, also serves as an internal control for potential artefacts associated with MO injection.

Figure 1
L–R reversal of anatomical asymmetry in larval zebrafish. (a,b) Dorsal views of the pineal and asymmetrically positioned parapineal (arrowhead) at 3 dpf, following injection of the southpaw MO into the Tg(foxd3:GFP)fkg17 (Gilmour et al. 2002) ...

3. Epithalamic reversal does not affect motor responses

To test whether sensory and motor responses differ between the four anatomical groups, we took advantage of the Flote automated system for high-speed video recording and analysis. Flote was designed to measure the detailed kinematics of individual motor behaviours simultaneously in groups of larvae, in an observer-independent manner (Burgess & Granato 2007a). We first examined whether pre-sorted parapineal and pancreas reversed (RppLpa) or discordant (LppLpa and RppRpa) larvae showed differences from the LppRpa group in the directionality of their spontaneous movements. To assess spontaneous movements, groups of 7 dpf larvae (8–10 per group) were pre-adapted to a set level of light (170 μW cm−2) consistent with the intensity of illumination in the testing arena. After dishes were transferred to the testing arena, larvae were given 3 min to stabilize the levels of locomotor activity prior to video recording. Under unperturbed conditions, larvae typically swim in bouts of forward-directed movements termed ‘slow swims’ or ‘scoots’ and also execute reorienting movements referred to as ‘routine turns’ (R-turns; Budick & O'Malley 2000; Burgess & Granato 2007b). For each anatomical group tested, the kinematics of turning were normal (data not shown) and there was no difference between the groups in the percentage of R-turns executed in a rightward direction (no effect of parapineal laterality (F1,4=0.39, p=0.56) or visceral laterality (F1,4=0.003, p=0.96) using two-way ANOVA). Combining all groups, 50.3±3.2% of R-turns were initiated in a rightward direction (one-sample t-test for 50%; t7=0.11, p=0.93), indicating that there was no intrinsic L–R bias in turning behaviour under baseline conditions.

We measured the responsiveness and kinematics of larval startle responses following exposure to an intense acoustic/vibrational stimulus (refer to Burgess & Granato (2007a) for details of the startle paradigm). Zebrafish larvae have two primary stereotyped response modes to an acoustic startle stimulus, an explosive C-bend with a short latency (4–8 ms, short latency C-start or SLC) and a second type of C-bend initiated with slower and prolonged duration and with a much longer latency (20–50 ms, long-latency C-start or LLC) (Kimmel et al. 1974; Burgess & Granato 2007a). Both responses are followed by burst swimming movements, which rapidly propel larvae away from their initial position.

In spaw MO-injected larvae, no differences were found in the initiation frequency for either SLC responses (F3,68=0.80, p=0.50; figure 2a) or LLC responses (F3,68=0.52, p=0.66; figure 2b) between the four anatomical classes. The kinematics of the SLC and LLC responses were also indistinguishable. For example, for the first C-bend of the LLC responses, the latency (F3,67=0.30, p=0.83), magnitude (F3,67=1.13, p=0.34), duration (F3,67=1.69, p=0.18) and angular velocity (F3,67=0.57, p=0.63) showed no group effect, nor was any group significantly different by t-test from the LppRpa group. These results indicate that all larvae, regardless of their anatomical laterality, sense the startle stimulus normally and respond with a stereotypic C-bend and characteristic succession of movements.

Figure 2
Equivalent startle responses in L–R reversed larvae. (a) Initiation frequencies for the short latency C-start (SLC) and (b) long latency C-start (LLC) responses. Movement initiation frequencies correspond to the percentage of trials in which SLC ...

As a population, wild-type zebrafish larvae do not show an intrinsic directional bias in the acoustic startle assay, with 50 per cent of both SLC and LLC responses being initiated in a rightward direction (Burgess & Granato 2007a). Directional bias was also not observed in spaw MO-injected LppRpa larvae for either mode of startle response, with 44.9±8.5% of SLC responses initiated in a rightward direction (one-sample t-test against 50%, t14=0.60, p=0.56) and 45.3±6.4% of LLC responses initiated rightward (t16=0.74, p=0.47). Moreover, there were no significant differences between the four anatomical groups for directionality of either SLC (F3,61=0.17, p=0.91) or LLC (F3,67=1.6, p=0.19) responses. Thus, parapineal or visceral asymmetry was not associated with a L–R bias in C-bends during the startle response.

4. Motor responses to directional stimuli

Next, we employed two tests in which motor responses of zebrafish larvae were directionally modulated by an asymmetrically presented stimulus, in the expectation that epithalamic reversal would disrupt lateralization of behavioural activity. For both assays, statistical analyses confirmed that visceral sidedness had no measurable effect, e.g. directionality of responses were not significantly different in either the dark flash test (independent samples t-test, t15=0.45, p=0.66) or the looming escape response (t7=1.4, p=0.21), allowing grouping of LppRpa with LppLpa and RppLpa with RppRpa into two datasets (refer to figure 3).

Figure 3
Directional behaviours are unaffected by epithalamic reversal. The (a) initiation frequency and (b) directionality of O-bend responses to dark flash stimuli were measured in Lpp and Rpp larvae (7 or 8 dpf). Dark flashes were generated as previously described ...

The first test used an abrupt reduction in illumination from an asymmetrically positioned light source (‘dark flash’). Wild-type larvae respond to a dark flash with a stereotyped movement initiated with a large amplitude C-bend (termed ‘O-bend’; Burgess & Granato 2007b). Because they tend to turn towards the extinguished light source (Burgess & Granato 2007b), directionality of an O-bend depends on which side of the larva initially faced the light.

Larvae with a left or right parapineal showed a similar level of responsiveness to a dark flash (independent samples t-test, t15=0.16, p=0.87; figure 3a) and O-bends were executed with equivalent kinematics in the two groups. For example, latency (Lpp=458±22 ms and Rpp=458±20 ms, t15=0.002, p=0.99) and C-magnitude (Lpp=141°±4°and Rpp=146°±4°, t15=0.90, p=0.38) were almost identical. The tendency of O-bends to be initiated towards the light source (‘bias’, figure 3b) was significant (one-sample t-test against 0, for Lpp t8=3.9, p=0.005 and for Rpp t7=4.3, p=0.004) and of similar magnitude for the two groups (t15=0.12, p=0.91).

The second test is based on the observation that many species of fishes, including adult zebrafish, are known to swim away from a looming object by reorienting in the same direction as the moving shadow, and then swimming vigorously forward (Dill 1974; Li & Dowling 1997). To assess the looming escape response, free-swimming larvae in a 6 cm dish were exposed to a moving shadow sweeping across the testing area at a constant rate. For each group of Lpp and Rpp larvae, eight repetitions of the looming stimulus were presented at 60 s intervals in alternating directions.

In this assay, larvae initiate turning manoeuvres to reorient away from the looming shadow, and then perform bouts of forward swimming in the same direction the shadow moves (H. Burgess & M. Granato 2007, unpublished observations). No significant difference in the frequency of turn initiations was detected between Lpp and Rpp larvae (independent samples t-test with unequal variance, t4.3=2.3, p=0.08; figure 3c). Moreover, the two groups showed very similar movement kinematics, including latency to movement (Lpp=412±28 ms and Rpp=395±25 ms, t7=0.46, p=0.66) and C-magnitude (Lpp=97±4° and Rpp=101±4°, t7=0.56, p=0.59). Thus, larvae with parapineal reversals both detect visual stimuli and have a normal magnitude of response. This assay also tests the directionality of response, as larvae show a strong bias to initiate turns away from the approaching shadow. Thus, larvae facing the shadow with their left side tend to turn rightward, whereas those facing the shadow with their right side primarily turn leftward. The directional bias of turn movements away from the shadow was almost identical in Lpp and Rpp larvae (t7=0.14, p=0.89; figure 3d). These experiments demonstrate that sensory acuity for acoustic and visual stimuli, movement kinematics and levels of responsiveness are all normal in larvae with parapineal reversals.

5. Larval populations do not show consistent eye preference

A behavioural test with inherent directionality is the choice of left or right eye used by a larva to view its mirror image. The procedure used to measure eye preference in zebrafish larva was adapted from the mirror test of Sovrano et al. (1999) for adult fish, and was similar to that described by Sovrano & Andrew (2006). At 8 dpf, each larva was tested individually by gently placing it in the middle of a tank lined with mirrors and recording over a 5 min period its self-viewing approaches towards the mirrors using the left or right eye. Mock-injected larvae showed no population bias in eye use (n=50; one-sample t-test against 50%, t49=0.277, p=0.78; figure 4c). Transgenic larvae injected with spaw MO (n=200, 50 for each anatomical class; figure 4b) also did not exhibit statistically significant differences in eye use upon mirror image viewing (F3,199=2.03, p=0.11). To confirm this finding, we also examined uninjected transgenic larvae, screening through several thousands to identify the small number that showed spontaneous parapineal reversals (refer to figure 1e). As a group, neither RppLpa (n=28) nor RppRpa (n=37) larvae showed an eye preference in the mirror test and their viewing behaviour was indistinguishable from transgenic siblings with normal LppRpa (n=53) orientation (F2,117=1.41, p=0.25; figure 4c). In every control or experimental larval class, a subset did in fact show a left or right eye preference in mirror approaches (figure 4d,e); however, there was no consistent bias at the population level. While L–R eye use was measured over the entire 5 min period, larval viewing behaviours were also quantified during each 1 min interval, as previous work had suggested that larvae can shift their L–R preference over the course of testing (Barth et al. 2005; Sovrano & Andrew 2006). In a minute-by-minute analysis, Lpp and Rpp larvae also failed to exhibit a significant difference in L–R eye preference (figure 4f; interaction between time in minutes and laterality, F4,724=1.23, p=0.3).

Figure 4
Larval populations do not show consistent eye preference in the mirror test. (a) The mirror test is conducted in a rectangular tank (10×4 cm) with two mirrors as the longer walls and two white screens as the shorter walls. The tank contains ...

6. Parapineal reversed larvae exhibit navigational delay and reduced exploration

In the course of executing the mirror test, we observed that larvae with the right parapineal configuration showed a significant lag in the onset of navigation (Kruskal–Wallis test, Χ32=64.65, p<0.001; figure 5a). The onset was defined as the time that elapsed between the introduction of a larva into the testing chamber and its swimming a distance comparable to twice its body length. Swimming delay was unrelated to positioning of the viscera, as both RppLpa and RppRpa larvae had a pronounced lag of 66.6±9.2 and 54.9±7.68 s, respectively, compared to 13.5±2.5 s for LppRpa, 14.9±3.7 s for LppLpa and 4.67±1.05 for the mock-injected LppRpa group. Analyses of transgenic larvae with spontaneous parapineal reversals provided further support for a correlation with delayed navigational behaviour. Spontaneous RppLpa and RppRpa larvae also showed a significant lag in the onset of navigation compared to their LppRpa siblings (Kruskal–Wallis test, Χ22=45.54, p<0.001; figure 5b).

Figure 5
Larvae with reversed epithalamic asymmetry show altered navigational behaviour. (a) Mean and standard error of the elapsed time (in seconds) before a larva moves a distance equivalent to twice its body length in spaw MO-injected larvae. Differences between ...

By tracking movements over a 5 min period, we also measured the total distance covered by individual 8 dpf larvae (n=118, 35 LppRpa, 33 LppLpa, 30 RppRpa, 20 RppLpa) and their average speed for all swimming episodes. Not only do larvae with parapineal reversals exhibit a navigational delay compared to their left parapineal siblings, but they also cover far less territory (F3,117=8.15, p≤0.001; figure 5d) and show a reduced average swimming speed (F3,117=8.18, p<0.001; figure 5e). This finding was independent of visceral orientation (Scheffe post hoc test, p<0.001). A minute-by-minute analysis of the distance traversed (data not shown) indicates that the altered behaviour of Rpp larvae persists throughout the testing period (F19,569=9.89, p<0.001).

7. Discussion

The results from a battery of behavioural tests indicate that the motor responses of larval zebrafish with reversed laterality of the epithalamus and viscera are largely indistinguishable from those of their siblings with the predominant LppRpa anatomical configuration. Neither complete nor partial L–R reversals affect a larva's ability to react appropriately to acoustic and light stimuli; therefore, modified swimming behaviours cannot be accounted for merely by deficits in sensory processing, motor control or muscle activity.

Because all four classes of MO-injected individuals are viable and develop into fertile adults (Long et al. 2003; Gamse et al. 2005), it is unlikely that they harbour severe malformations, such as the vascular abnormalities that are frequently associated with situs defects in mammals (Icardo & Colvee 2001; Peeters & Devriendt 2006). We were concerned that altered visceral asymmetry might compromise swimming ability. However, opposite placement of the pancreas and liver (and presumably reversed directional coiling of the heart and intestines) in close to 50 per cent of larvae derived from spaw MO-injected embryos did not appear to modify spontaneous movements, the frequency or properties of C-bends during startle and escape responses, or the directional turning elicited by sudden changes in light. A probable reason for normal behavioural responses is that, even though the location and morphology of the heart and viscera are L–R reversed, the internal organs do not exhibit abnormal positioning with respect to one another (e.g. situs ambiguous or heterotaxia). For example, at 6 dpf, we never observed larvae that had their liver and pancreas positioned in the same orientation or both organs situated in the midline. In their initial description of spaw-depleted embryos, Long et al. (2003) found uncoupled defects in the directionality of the jogging and looping stages of heart tube morphogenesis, but they did not report whether these changes were concordant with L–R positioning of the pancreas or other visceral organs. An unaccounted for observation, however, is that the LppLpa group was always significantly underrepresented following MO injection. LppLpa larvae have also not been spontaneously recovered from wild-type populations. There may be an early developmental disadvantage for this configuration compared to the other groups, although this has not been directly determined.

We and others had previously shown that the position of the parapineal is tightly coupled to the directional asymmetry of the paired habenular nuclei, including differences in their size, amount of dense neuropil, gene expression and innervation of their shared midbrain target, the IPN (Concha et al. 2000, 2003; Gamse et al. 2003, 2005; Aizawa et al. 2005; Kuan et al. 2007a,b). Thus, reversal of parapineal position, which is typically observed in 2–3% of larvae from wild-type strains, is a readily scored indicator of more pronounced changes in the epithalamus and in epithalamic connectivity. However, whether the position of the parapineal represents directional asymmetry throughout the nervous system in either natural or genetically manipulated populations remains to be demonstrated. It may not be the case that a reversal in parapineal position is indicative of reversed asymmetry throughout the brain or predictive of a corresponding shift in lateralized behaviours. Indeed, L–R reversed fsi larvae also exhibited some lateralized behaviours with normal directionality (Barth et al. 2005; Andrew 2006).

Although previous studies have indicated that adult and larval zebrafish as well as many other teleost species exhibit a left eye bias upon self-viewing (Sovrano et al. 1999, 2001; De Santi et al. 2001; Watkins et al. 2004), we recorded no baseline difference in eye preference in the doubly transgenic larvae used in this study. Analyses of Lpp and Rpp larvae from the fsi strain indicated that they exhibited opposite eye preference upon mirror viewing and an inverse shift in eye preference occurred over time in both groups (Barth et al. 2005). We did not find evidence for similar population biases in eye use for any of the spaw MO-injected groups. Moreover, transgenic larvae we collected that showed spontaneous parapineal reversals also did not demonstrate a statistically significant bias in L–R eye preference. In addition, we have not observed other behavioural asymmetries during responses to a variety of directional and non-directional stimuli.

A simple explanation for these apparently conflicting results is the existence of variability between zebrafish strains. The transgenic lines used in this study have complex genetic backgrounds, as they were initially produced in undefined fish strains (Wan et al. 2006) or in the golden pigment mutant (Gilmour et al. 2002), and maintained in our aquatics facility through outcrosses to the Oregon AB line (Walker 1999), followed by intercrosses to preserve transgene homozygosity. Behavioural differences between strains of zebrafish have been previously observed in the mirror test (Sovrano & Andrew 2006; Andrew et al. in press), and could explain why we did not obtain evidence for consistent eye preference at the population level. The fact that some individuals in all laterality groups did demonstrate a left or right bias indicates that our testing paradigm for self-image viewing was a robust assay and was unlikely to be the source of the observed discrepancy between our results and prior work. Not only do strain differences exist in L–R eye preference, but it has also been suggested that single larvae modify their eye use for self-viewing during the course of a testing session, as their familiarity with the apparatus and visual stimuli increases. However, we did not find evidence for minute-by-minute changes in eye preference for any of the anatomical classes tested.

Another possible explanation for the differences observed between studies is that zebrafish larvae identified fortuitously in control populations, from strains with an enhanced predisposition for L–R reversals (Barth et al. 2005), or following genetic manipulations such as spaw MO injection, may not be morphologically identical. While this hypothesis cannot be ruled out, we do not favour it, as Rpp larvae showed very similar viewing behaviour irrespective of their derivation from injected or uninjected transgenic embryos. Moreover, spaw expression is restricted to the caudal region and left lateral plate mesoderm of developing embryos and has not been detected in the nervous system (Long et al. 2003). It is therefore unlikely that the spaw antisense MO would directly perturb brain development outside of its effect on L–R determination. Similarly, the fsi strain has only been described as increasing the frequency of concordant visceral and epithalamic reversals and has not been associated with other developmental defects (Barth et al. 2005). The ability to generate large numbers of parapineal-reversed larvae using spaw MO should enable strain differences in mirror image viewing to be examined more rigorously and, perhaps, in parallel with tests on individuals from the fsi strain.

In our study, all larval groups displayed similar responsiveness and kinematics in tests for motor responses. Thus, it may appear contradictory that Rpp larvae showed a delay in the onset of movement and reduced overall swimming in the mirror test. However, there are important operational differences between these behavioural assays. Testing of rapid kinematic responses to acute stimuli is performed simultaneously on small groups of larvae in a pre-adapted environment. The mirror testing chamber provides an unfamiliar environment, one in which individually assayed larvae repeatedly encounter their reflection and have an increased area to explore.

We propose that these differences account for the behavioural response, in that a Rpp larva, while possessing normal motor reactivity, appears less motivated or more fearful to initiate exploration in a novel environment. Recent work in mammals has uncovered an interesting link between the habenular region and control of the dopaminergic mesolimbic pathway that mediates fear, motivation and reward (Heldt & Ressler 2006; Morissette & Boye 2008). Specifically, the lateral habenula nucleus was found to provide inhibitory signals to dopaminergic neurons in the ventral midbrain (Matsumoto & Hikosaka 2007). Midbrain dopaminergic neurons in turn send input to the limbic system and, notably, to the amygdala and nucleus accumbens, brain areas implicated in fear and reward (Di Chiara & Bassareo 2007; LeDoux 2007). The lateral habenular nuclei also receive substantial dopaminergic input, suggesting a further level of cross-regulation (Gruber et al. 2007). Zebrafish seem to lack structures equivalent to the lateral habenula (Concha & Wilson 2001); however, as in other recent studies, comparative gene expression analyses may identify brain regions that are functionally homologous with mammals (Wullimann & Rink 2002; Mueller et al. 2008). Moreover, there is recent evidence from rats that the medial habenula and IPN are also involved in modulating the dopaminergic pathway (Taraschenko et al. 2007a,b). Intriguingly, the firing rates of neurons in the medial and lateral habenulae, as well as the IPN, closely correspond with locomotor activity in rats (Sharp et al. 2006).

In zebrafish, a mesolimbic-like circuit is present in larvae and adults, although there are some differences in the location of dopaminergic neurons (Rink & Wullimann 2002). Pharmacological studies have also implicated dopamine in the control of larval locomotor activity (Giacomini et al. 2006; Boehmler et al. 2007; Thirumalai & Cline 2008). It will be of great interest to examine whether the altered exploratory behaviour of parapineal-reversed larvae is caused by changes in the differentiation, connectivity or function of dopaminergic neurons. However, why L–R reversal of habenular identity and efferent projections to the dorsal and ventral IPN would disrupt this proposed modulatory function is unclear.

In addition to modulating the dopaminergic pathway, the habenulo-interpeduncular system has been implicated in regulating monoaminergic and cholinergic transmission in the mammalian brain, and in functions as diverse as olfaction, feeding, mating, nociception, attention, sleep/wake cycling, stress, fear and learning (reviewed in Sutherland 1982; Klemm 2004; LeCourtier & Kelly 2007). To assess behavioural impact, lesioning of the habenulae in rats or mice is typically performed, but experimental approaches often do not discriminate between the medial and lateral habenular nuclei or take their complex subnuclear organization into account. Notwithstanding these caveats, impairments in attention, learning and memory have been widely documented. For instance, habenular-lesioned animals have difficulty in learning conditioned avoidance to aversive stimuli (Rausch & Long 1974) and show a marked increase in premature responses (Sasaki et al. 1990). Following habenular lesions, rats also respond prematurely in a spatial learning paradigm, suggesting that behaviour becomes more impulsive (LeCourtier & Kelly 2005). In some cognitive assays, the effect of habenular loss is enhanced if stress levels are increased (Thornton & Bradbury 1989; Heldt & Ressler 2006). There is also evidence that habenular neurons respond to retinal illumination and may serve to link circadian and motivational pathways in the brain (Zhao & Rusak 2005).

An essential goal for future studies in the zebrafish will be to learn more about the targets of the IPN and how L–R reversal of habenular connections with the IPN might influence neuronal activity elsewhere in the brain. Although the habenulo-IPN projection is highly conserved across vertebrates (Sutherland 1982; Concha & Wilson 2001), knowledge of its integration with other conduction systems is lacking. Without this information, it will remain a challenge to understand why epithalamic laterality evolved and persisted in fishes, amphibians and reptiles. In addition, even though pineal-associated structures and the habenulae are asymmetric in these species (Concha & Wilson 2001), only fishes seem to exhibit differential innervation of the dorsal and ventral IPN by left and right habenular neurons (Kuan et al. 2007a,b). A further mystery is why morphological differences between the left and right habenular nuclei are rarely found in mammals (Sutherland 1982), suggesting that functional specialization of this part of the brain may be more important for aquatic species.

While the behaviours associated with epithalamic L–R asymmetry may prove more complicated and variable than previously appreciated, the zebrafish model has emerged as a valuable system for genetic manipulation of asymmetry, analyses of neuroanatomical development and connectivity and the application of diverse functional assays to tackle this exciting problem.

Acknowledgments

Protocols for use of zebrafish were approved by the Institutional Animal Care and Use Committee of the Carnegie Institution Department of Embryology.

This work was supported in part by an NRSA postdoctoral fellowship to H.A.B. and grants to M.G. from the National Institutes of Health (MH075691 and HD 37975). L.F. and M.E.H. gratefully acknowledge funds from the Eppley Foundation for Research, which partially supported this study. We thank Michelle Macurak and Lea Fortuno for technical assistance, Brian Hollenback and Nicole Gabriel for animal care, Allen Strause for manufacturing testing chambers, Fouad Siddiqi for assistance with Matlab, and Courtney Akitake and Mary Goll for their help with figure 1. We also thank Steve Leach (Johns Hopkins Medical Institutions, Baltimore) and Darren Gilmour (European Molecular Biology Laboratory, Heidelberg) for generously providing transgenic zebrafish lines.

Footnotes

One contribution of 14 to a Theme Issue ‘Mechanisms and functions of brain and behavioural asymmetries’.

References

  • Aizawa H., Bianco I.H., Hamaoka T., Miyashita T., Uemura O., Concha M.L., Russell C., Wilson S.W., Okamoto H. Laterotopic representation of left–right information onto the dorso-ventral axis of a zebrafish midbrain target nucleus. Curr. Biol. 2005;15:238–243. doi:10.1016/j.cub.2005.01.014 [PMC free article] [PubMed]
  • Aizawa H., Goto M., Sato T., Okamoto H. Temporally regulated asymmetric neurogenesis causes left–right difference in the zebrafish habenular structures. Dev. Cell. 2007;12:87–98. doi:10.1016/j.devcel.2006.10.004 [PubMed]
  • Andrew R.J. Partial reversal of the brain generates new behavioural phenotypes. Cortex. 2006;42:110–112. doi:10.1016/S0010-9452(08)70333-2 [PubMed]
  • Andrew, R. J., Dharmaretnam, M., Gyõri, B., Miklósi, A., Watkins, J. & Sovrano, V. A. In press. Precise endogenous control of involvement of right and left visual structures in assessment by zebrafish. Behav. Brain Res [PubMed]
  • Barth K.A., Miklósi A., Watkins J., Bianco I.H., Wilson S.W., Andrew R.J. fsi zebrafish show concordant reversal of laterality of viscera, neuroanatomy, and a subset of behavioral responses. Curr. Biol. 2005;15:844–850. doi:10.1016/j.cub.2005.03.047 [PMC free article] [PubMed]
  • Bianco I.H., Carl M., Russell C., Clarke J.D., Wilson S.W. Brain asymmetry is encoded at the level of axon terminal morphology. Neural Dev. 2008;3:9. [PMC free article] [PubMed]
  • Bisazza A., de Santi A. Lateralization of aggression in fish. Behav. Brain Res. 2003;141:131–136. doi:10.1016/S0166-4328(02)00344-3 [PubMed]
  • Bisazza A., Rogers L.J., Vallortigara G. The origins of cerebral asymmetry: a review of evidence of behavioural and brain lateralization in fishes, reptiles and amphibians. Neurosci. Biobehav. Rev. 1998;22:411–426. doi:10.1016/S0149-7634(97)00050-X [PubMed]
  • Bisazza A., Cantalupo C., Capocchiano M., Vallortigara G. Population lateralisation and social behaviour: a study with 16 species of fish. Laterality. 2000;5:269–284. doi:10.1080/135765000406111 [PubMed]
  • Bisazza A., Sovrano V.A., Vallortigara G. Consistency among different tasks of left–right asymmetries in lines of fish originally selected for opposite direction of lateralization in a detour task. Neuropsychologia. 2001;39:1077–1085. doi:10.1016/S0028-3932(01)00034-3 [PubMed]
  • Bisgrove B.W., Snarr B.S., Emrazian A., Yost H.J. Polaris and polycystin-2 in dorsal forerunner cells and Kupffer's vesicle are required for specification of the zebrafish left–right axis. Dev. Biol. 2005;287:274–288. doi:10.1016/j.ydbio.2005.08.047 [PubMed]
  • Boehmler W., Carr T., Thisse C., Thisse B., Canfield V.A., Levenson R. D4 Dopamine receptor genes of zebrafish and effects of the antipsychotic clozapine on larval swimming behaviour. Genes Brain Behav. 2007;6:155–166. doi:10.1111/j.1601-183X.2006.00243.x [PubMed]
  • Budick S.A., O'Malley D.M. Locomotor repertoire of the larval zebrafish: swimming, turning and prey capture. J. Exp. Biol. 2000;203:2565–2579. [PubMed]
  • Burgess H.A., Granato M. Sensorimotor gating in larval zebrafish. J. Neurosci. 2007a;27:4984–4994. doi:10.1523/JNEUROSCI.0615-07.2007 [PubMed]
  • Burgess H.A., Granato M. Modulation of locomotor activity in larval zebrafish during light adaptation. J. Exp. Biol. 2007b;210:2526–2539. doi:10.1242/jeb.003939 [PubMed]
  • Carl M., Bianco I.H., Bajoghli B., Aghaallaei N., Czerny T., Wilson S.W. Wnt/Axin1/beta-catenin signaling regulates asymmetric nodal activation, elaboration, and concordance of CNS asymmetries. Neuron. 2007;55:393–405. doi:10.1016/j.neuron.2007.07.007 [PMC free article] [PubMed]
  • Concha M.L., Wilson S.W. Asymmetry in the epithalamus of vertebrates. J. Anat. 2001;199:63–84. doi:10.1017/S0021878201008329 [PubMed]
  • Concha M.L., Burdine R.D., Russell C., Schier A.F., Wilson S.W. A nodal signaling pathway regulates the laterality of neuroanatomical asymmetries in the zebrafish forebrain. Neuron. 2000;28:399–409. doi:10.1016/S0896-6273(00)00120-3 [PubMed]
  • Concha M.L., et al. Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality. Neuron. 2003;39:423–438. doi:10.1016/S0896-6273(03)00437-9 [PubMed]
  • De Santi A., Sovrano V.A., Bisazza A., Vallortigara G. Mosquitofish display differential left- and right-eye use during mirror image scrutiny and predator inspection responses. Anim. Behav. 2001;61:305–310. doi:10.1006/anbe.2000.1566
  • Di Chiara G., Bassareo V. Reward system and addiction: what dopamine does and doesn't do. Curr. Opin. Pharmacol. 2007;7:69–76. doi:10.1016/j.coph.2006.11.003 [PubMed]
  • Dill L.M. The escape response of the zebra danio (Bracydanio rerio). I. The stimulus for escape. Anim. Behav. 1974;22:710–721.
  • Dong P.D., Munson C.A., Norton W., Crosnier C., Pan X., Gong Z., Neumann C.J., Stainier D.Y. Fgf10 regulates hepatopancreatic ductal system patterning and differentiation. Nat. Genet. 2007;39:397–402. doi:10.1038/ng1961 [PubMed]
  • Essner J.J., Branford W.W., Zhang J., Yost H.J. Mesendoderm and left–right brain, heart and gut development are differentially regulated by pitx2 isoforms. Development. 2000;127:1081–1093. [PubMed]
  • Essner J.J., Amack J.D., Nyholm M.K., Harris E.B., Yost H.J. Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left–right development of the brain, heart and gut. Development. 2005;132:1247–1260. doi:10.1242/dev.01663 [PubMed]
  • Facchin L., Bisazza A., Vallortigara G. What causes lateralization of detour behavior in fish? Evidence for asymmetries in eye use. Behav. Brain Res. 1999;103:229–234. doi:10.1016/S0166-4328(99)00043-1 [PubMed]
  • Gamse J.T., Shen Y.C., Thisse C., Thisse B., Raymond P.A., Halpern M.E., Liang J.O. Otx5 regulates genes that show circadian expression in the zebrafish pineal complex. Nat. Genet. 2002;30:117–121. doi:10.1038/ng793 [PubMed]
  • Gamse J.T., Thisse C., Thisse B., Halpern M.E. The parapineal mediates left–right asymmetry in the zebrafish diencephalon. Development. 2003;130:1059–1068. doi:10.1242/dev.00270 [PubMed]
  • Gamse J.T., Kuan Y.S., Macurak M., Brosamle C., Thisse B., Thisse C., Halpern M.E. Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target. Development. 2005;132:4869–4881. doi:10.1242/dev.02046 [PubMed]
  • Giacomini N.J., Rose B., Kobayashi K., Guo S. Antipsychotics produce locomotor impairment in larval zebrafish. Neurotoxicol. Teratol. 2006;28:245–250. doi:10.1016/j.ntt.2006.01.013 [PubMed]
  • Gilmour D.T., Maischein H.M., Nusslein-Volhard C. Migration and function of a glial subtype in the vertebrate peripheral nervous system. Neuron. 2002;34:577–588. doi:10.1016/S0896-6273(02)00683-9 [PubMed]
  • Gruber C., Kahl A., Lebenheim L., Kowski A., Dittgen A., Veh R.W. Dopaminergic projections from the VTA substantially contribute to the mesohabenular pathway in the rat. Neurosci. Lett. 2007;427:165–170. doi:10.1016/j.neulet.2007.09.016 [PubMed]
  • Güntürkün O., Diekamp B., Manns M., Nottelmann F., Prior H., Schwarz A., Skiba M. Asymmetry pays: visual lateralization improves discrimination success in pigeons. Curr. Biol. 2000;10:1079–1081. doi:10.1016/S0960-9822(00)00671-0 [PubMed]
  • Heldt S.A., Ressler K.J. Lesions of the habenula produce stress- and dopamine-dependent alterations in prepulse inhibition and locomotion. Brain Res. 2006;1073–1074:229–239. doi:10.1016/j.brainres.2005.12.053 [PMC free article] [PubMed]
  • Heuts B.A. Lateralization of trunk muscle volume, and lateralization of swimming turns of fish responding to external stimuli. Behav. Process. 1999;47:113–124. doi:10.1016/S0376-6357(99)00056-X [PubMed]
  • Icardo J.M., Colvee E. Origin and course of the coronary arteries in normal mice and in iv/iv mice. J. Anat. 2001;199:473–482. doi:10.1046/j.1469-7580.2001.19940473.x [PubMed]
  • Inbal A., Kim S.H., Shin J., Solnica-Krezel L. Six3 represses nodal activity to establish early brain asymmetry in zebrafish. Neuron. 2007;55:407–415. doi:10.1016/j.neuron.2007.06.037 [PMC free article] [PubMed]
  • Kimmel C.B., Patterson J., Kimmel R.O. The development and behavioral characteristics of the startle response in the zebra fish. Dev. Psychobiol. 1974;7:47–60. doi:10.1002/dev.420070109 [PubMed]
  • Klemm W.R. Habenular and interpeduncularis nuclei: shared components in multiple-function networks. Med. Sci. Monit. 2004;10:261–273. [PubMed]
  • Kuan Y.S., Gamse J.T., Schreiber A.M., Halpern M.E. Selective asymmetry in a conserved forebrain to midbrain projection. J. Exp. Zool. B Mol. Dev. Evol. 2007a;308:669–678. doi:10.1002/jez.b.21184 [PubMed]
  • Kuan Y.S., Yu H.H., Moens C.B., Halpern M.E. Neuropilin asymmetry mediates a left–right difference in habenular connectivity. Development. 2007b;134:857–865. doi:10.1242/dev.02791 [PubMed]
  • LeCourtier L., Kelly P.H. Bilateral lesions of the habenula induce attentional disturbances in rats. Neuropsychopharmacology. 2005;30:484–496. doi:10.1038/sj.npp.1300595 [PubMed]
  • LeCourtier L., Kelly P.H. A conductor hidden in the orchestra? Role of the habenular complex in monoamine transmission and cognition. Neurosci. Biobehav. Rev. 2007;31:658–672. doi:10.1016/j.neubiorev.2007.01.004 [PubMed]
  • LeDoux J. The amygdala. Curr. Biol. 2007;17:R868–R874. doi:10.1016/j.cub.2007.08.005 [PubMed]
  • Li L., Dowling J.E. A dominant form of inherited retinal degeneration caused by a non-photoreceptor cell-specific mutation. Proc. Natl Acad. Sci. USA. 1997;94:11 645–11 650. doi:10.1073/pnas.94.21.11645 [PubMed]
  • Liang J.O., Rubinstein A.L. Patterning of the zebrafish embryo by nodal signals. Curr. Top. Dev. Biol. 2003;55:143–171. doi:10.1016/S0070-2153(03)01003-2 [PubMed]
  • Liang J.O., Etheridge A., Hantsoo L., Rubinstein A.L., Nowak S.J., Izpisua Belmonte J.C., Halpern M.E. Asymmetric nodal signaling in the zebrafish diencephalon positions the pineal organ. Development. 2000;127:5101–5112. [PubMed]
  • Long S., Ahmad N., Rebagliati M. The zebrafish nodal-related gene southpaw is required for visceral and diencephalic left–right asymmetry. Development. 2003;130:2303–2316. doi:10.1242/dev.00436 [PubMed]
  • Matsumoto M., Hikosaka O. Lateral habenula as a source of negative reward signals in dopamine neurons. Nature. 2007;447:1111–1115. doi:10.1038/nature05860 [PubMed]
  • Miklósi A., Andrew R.J. Right eye use associated with decision to bite in zebrafish. Behav. Brain Res. 1999;105:199–205. doi:10.1016/S0166-4328(99)00071-6 [PubMed]
  • Miklósi A., Andrew R.J. The zebrafish as a model for behavioral studies. Zebrafish. 2006;3:227–234. doi:10.1089/zeb.2006.3.227 [PubMed]
  • Miklósi A., Andrew R.J., Savage H. Behavioural lateralisation of the tetrapod type in the zebrafish (Brachydanio rerio) Physiol. Behav. 1997;63:127–135. doi:10.1016/S0031-9384(97)00418-6 [PubMed]
  • Miklósi A., Andrew R.J., Gasparini S. Role of right hemifield in visual control of approach to target in zebrafish. Behav. Brain Res. 2001;122:57–65. doi:10.1016/S0166-4328(01)00167-X [PubMed]
  • Morissette M.C., Boye S.M. Electrolytic lesions of the habenula attenuate brain stimulation reward. Behav. Brain Res. 2008;187:17–26. doi:10.1016/j.bbr.2007.08.021 [PubMed]
  • Mueller T., Wullimann M.F., Guo S. Early teleostean basal ganglia development visualized by zebrafish Dlx2a, Lhx6, Lhx7, Tbr2 (eomesa), and GAD67 gene expression. J. Comp. Neurol. 2008;507:1245–1257. doi:10.1002/cne.21604 [PubMed]
  • Nüsslein-Volhard C., Dahm R. Oxford University Press; Oxford, UK: 2002. Zebrafish a practical approach.
  • Peeters H., Devriendt K. Human laterality disorders. Eur. J. Med. Genet. 2006;49:349–362. doi:10.1016/j.ejmg.2005.12.003 [PubMed]
  • Rausch L.J., Long C.J. Habenular lesions and discrimination responding to olfactory and visual stimuli. Physiol. Behav. 1974;13:357–364. doi:10.1016/0031-9384(74)90088-2 [PubMed]
  • Reddon A.R., Hurd P.L. Aggression, sex and individual differences in cerebral lateralization in a cichlid fish. Biol. Lett. 2008;4:338–340. doi:10.1098/rsbl.2008.0206 [PMC free article] [PubMed]
  • Rink E., Wullimann M.F. Development of the catecholaminergic system in the early zebrafish brain: an immunohistochemical study. Brain Res. Dev. Brain Res. 2002;137:89–100. doi:10.1016/S0165-3806(02)00354-1 [PubMed]
  • Rogers L.J. Development and function of lateralization in the avian brain. Brain Res. Bull. 2008;76:235–244. doi:10.1016/j.brainresbull.2008.02.001 [PubMed]
  • Sampath K., Rubinstein A.L., Cheng A.M., Liang J.O., Fekany K., Solnica-Krezel L., Korzh V., Halpern M.E., Wright C.V. Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling. Nature. 1998;395:185–189. doi:10.1038/26020 [PubMed]
  • Sasaki K., Suda H., Watanabe H., Yagi H. Involvement of the entopeduncular nucleus and the habenula in methamphetamine-induced inhibition of dopamine neurons in the substantia nigra of rats. Brain Res. Bull. 1990;25:121–127. doi:10.1016/0361-9230(90)90262-X [PubMed]
  • Schier A.F. Nodal signaling in vertebrate development. Annu. Rev. Cell Dev. Biol. 2003;19:589–621. doi:10.1146/annurev.cellbio.19.041603.094522 [PubMed]
  • Sharp P.E., Turner-Williams S., Tuttle S. Movement-related correlates of single cell activity in the interpeduncular nucleus and habenula of the rat during a pellet-chasing task. Behav. Brain Res. 2006;166:55–70. doi:10.1016/j.bbr.2005.07.004 [PubMed]
  • Snelson C.D., Santhakumar K., Halpern M.E., Gamse J.T. Tbx2b is required for the development of the parapineal organ. Development. 2008;135:1693–1702. doi:10.1242/dev.016576 [PMC free article] [PubMed]
  • Sovrano V.A., Andrew R.J. Eye use during viewing a reflection: behavioural lateralisation in zebrafish larvae. Behav. Brain Res. 2006;167:226–231. doi:10.1016/j.bbr.2005.09.021 [PubMed]
  • Sovrano V.A., Rainoldi C., Bisazza A., Vallortigara G. Roots of brain specializations: preferential left-eye use during mirror-image inspection in six species of teleost fish. Behav. Brain Res. 1999;106:175–180. doi:10.1016/S0166-4328(99)00105-9 [PubMed]
  • Sovrano V.A., Bisazza A., Vallortigara G. Lateralization of response to social stimuli in fishes: a comparison between different methods and species. Physiol. Behav. 2001;74:237–244. doi:10.1016/S0031-9384(01)00552-2 [PubMed]
  • Sutherland R.J. The dorsal diencephalic conduction system: a review of the anatomy and functions of the habenular complex. Neurosci. Biobehav. Rev. 1982;6:1–13. doi:10.1016/0149-7634(82)90003-3 [PubMed]
  • Taraschenko O.D., Rubbinaccio H.Y., Shulan J.M., Glick S.D., Maisonneuve I.M. Morphine-induced changes in acetylcholine release in the interpeduncular nucleus and relationship to changes in motor behavior in rats. Neuropharmacology. 2007a;53:18–26. doi:10.1016/j.neuropharm.2007.04.010 [PMC free article] [PubMed]
  • Taraschenko O.D., Shulan J.M., Maisonneuve I.M., Glick S.D. 18-MC acts in the medial habenula and interpeduncular nucleus to attenuate dopamine sensitization to morphine in the nucleus accumbens. Synapse. 2007b;61:547–560. doi:10.1002/syn.20396 [PubMed]
  • Thirumalai V., Cline H.T. Endogenous dopamine suppresses initiation of swimming in pre-feeding zebrafish larvae. J. Neurophysiol. 2008;100:1635–1648. doi:10.1152/jn.90568.2008 [PubMed]
  • Thornton E.W., Bradbury G.E. Effort and stress influence the effect of lesion of the habenula complex in one-way active avoidance learning. Physiol. Behav. 1989;45:929–935. doi:10.1016/0031-9384(89)90217-5 [PubMed]
  • Vallortigara G. Comparative neuropsychology of the dual brain: a stroll through animals' left and right perceptual worlds. Brain Lang. 2000;73:189–219. doi:10.1006/brln.2000.2303 [PubMed]
  • Vallortigara G., Bisazza A. How ancient is brain lateralization? In: Rogers L.J., Andrew R.J., editors. Comparative vertebrate lateralization. Cambridge University Press; Cambridge, UK: 2002. pp. 9–69.
  • Vallortigara G., Rogers L.J. Survival with an asymmetrical brain: advantages and disadvantages of cerebral lateralization. Behav. Brain Sci. 2005;28:575–589. discussion 589–633. [PubMed]
  • Walker C. Haploid screens and gamma-ray mutagenesis. Methods Cell Biol. 1999;60:43–70. doi:10.1016/S0091-679X(08)61893-2 [PubMed]
  • Wan H., Korzh S., Li Z., Mudumana S.P., Korzh V., Jiang Y.J., Lin S., Gong Z. Analyses of pancreas development by generation of gfp transgenic zebrafish using an exocrine pancreas-specific elastaseA gene promoter. Exp. Cell Res. 2006;312:1526–1539. doi:10.1016/j.yexcr.2006.01.016 [PubMed]
  • Watkins J., Miklósi A., Andrew R.J. Early asymmetries in the behaviour of zebrafish larvae. Behav. Brain Res. 2004;151:177–183. doi:10.1016/j.bbr.2003.08.012 [PubMed]
  • Wullimann M.F., Rink E. The teleostean forebrain: a comparative and developmental view based on early proliferation, Pax6 activity and catecholaminergic organization. Brain Res. Bull. 2002;57:363–370. doi:10.1016/S0361-9230(01)00666-9 [PubMed]
  • Zhao H., Rusak B. Circadian firing-rate rhythms and light responses of rat habenular nucleus neurons in vivo and in vitro. Neuroscience. 2005;132:519–528. doi:10.1016/j.neuroscience.2005.01.012 [PubMed]

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