PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
 
BMC Genomics. 2009; 10: 109.
Published online Mar 15, 2009. doi:  10.1186/1471-2164-10-109
PMCID: PMC2664823
Identification of candidate genes for human pituitary development by EST analysis
Yueyun Ma,#1 Xiaofei Qi,#2 Jianjun Du,#3 Shaojun Song,4 Dongyun Feng,4 Jia Qi,2 Zhidong Zhu,2 Xin Zhang,2 Huasheng Xiao,2 Zeguang Han,corresponding author2 and Xiaoke Haocorresponding author1
1Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China
2Chinese National Human Genome Center at Shanghai, 351 Guo Shou-Jing Road, Shanghai 201203, PR China
3Department of General Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China
4Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China
corresponding authorCorresponding author.
#Contributed equally.
Yueyun Ma: cmbmayy/at/fmmu.edu.cn; Xiaofei Qi: qisha8888/at/sina.com; Jianjun Du: dujjp/at/hotmail.com; Shaojun Song: qxsung/at/fmmu.edu.cn; Dongyun Feng: fengdy/at/fmmu.edu.cn; Jia Qi: qijia/at/chgc.sh.cn; Zhidong Zhu: zhuzd/at/chgc.sh.cn; Xin Zhang: zhangxin/at/chgc.sh.cn; Huasheng Xiao: xiaohs/at/ion.ac.cn; Zeguang Han: hanzg/at/chgc.sh.cn; Xiaoke Hao: haoxkg/at/fmmu.edu.cn
Received June 18, 2008; Accepted March 15, 2009.
Abstract
Background
The pituitary is a critical neuroendocrine gland that is comprised of five hormone-secreting cell types, which develops in tandem during the embryonic stage. Some essential genes have been identified in the early stage of adenohypophysial development, such as PITX1, FGF8, BMP4 and SF-1. However, it is likely that a large number of signaling molecules and transcription factors essential for determination and terminal differentiation of specific cell types remain unidentified. High-throughput methods such as microarray analysis may facilitate the measurement of gene transcriptional levels, while Expressed sequence tag (EST) sequencing, an efficient method for gene discovery and expression level analysis, may no-redundantly help to understand gene expression patterns during development.
Results
A total of 9,271 ESTs were generated from both fetal and adult pituitaries, and assigned into 961 gene/EST clusters in fetal and 2,747 in adult pituitary by homology analysis. The transcription maps derived from these data indicated that developmentally relevant genes, such as Sox4, ST13 and ZNF185, were dominant in the cDNA library of fetal pituitary, while hormones and hormone-associated genes, such as GH1, GH2, POMC, LHβ, CHGA and CHGB, were dominant in adult pituitary. Furthermore, by using RT-PCR and in situ hybridization, Sox4 was found to be one of the main transcription factors expressed in fetal pituitary for the first time. It was expressed at least at E12.5, but decreased after E17.5. In addition, 40 novel ESTs were identified specifically in this tissue.
Conclusion
The significant changes in gene expression in both tissues suggest a distinct and dynamic switch between embryonic and adult pituitaries. All these data along with Sox4 should be confirmed to further understand the community of multiple signaling pathways that act as a cooperative network that regulates maturation of the pituitary. It was also suggested that EST sequencing is an efficient means of gene discovery.
Articles from BMC Genomics are provided here courtesy of
BioMed Central