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J Clin Microbiol. 1987 April; 25(4): 748–750.
PMCID: PMC266077

Differential immunoglobulin G subclass antibody titers to respiratory syncytial virus F and G glycoproteins in adults.


Two respiratory syncytial virus glycoproteins, F and G, which differ substantially in the amount of glycosylation were used as antigens in an enzyme-linked immunosorbent assay to determine immunoglobulin G (IgG) subclass titers in 30 experimentally infected healthy adults. The titers of antibodies to the F glycoprotein achieved in postinfection sera were highest in the IgG1 subclass, whereas those to the G glycoprotein were highest and comparable in the IgG1 and IgG2 subclasses. The high IgG2 response to the G glycoprotein suggests that it is seen by the immune system as a polysaccharide antigen, a hypothesis consistent with its large carbohydrate content.

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  • Coleman RM, Nahmias AJ, Williams SC, Phillips DJ, Black CM, Reimer CB. IgG subclass antibodies to herpes simplex virus. J Infect Dis. 1985 May;151(5):929–936. [PubMed]
  • Collins PL, Huang YT, Wertz GW. Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7683–7687. [PubMed]
  • Dall'Olio F, Malagolini N, Speziali V, Campadelli-Fiume G, Serafini-Cessi F. Sialylated oligosaccharides O-glycosidically linked to glycoprotein C from herpes simplex virus type 1. J Virol. 1985 Oct;56(1):127–134. [PMC free article] [PubMed]
  • Elango N, Satake M, Coligan JE, Norrby E, Camargo E, Venkatesan S. Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit. Nucleic Acids Res. 1985 Mar 11;13(5):1559–1574. [PMC free article] [PubMed]
  • Fernie BF, Gerin JL. Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion. Infect Immun. 1982 Jul;37(1):243–249. [PMC free article] [PubMed]
  • Hammarström L, Granström M, Oxelius V, Persson MA, Smith CI. IgG subclass distribution of antibodies against S. aureus teichoic acid and alpha-toxin in normal and immunodeficient donors. Clin Exp Immunol. 1984 Mar;55(3):593–601. [PubMed]
  • Hornsleth A, Bech-Thomsen N, Friis B. Detection by ELISA of IgG-subclass-specific antibodies in primary respiratory syncytial (RS) virus infections. J Med Virol. 1985 Aug;16(4):321–328. [PubMed]
  • Julkunen I, Hovi T, Seppälä I, Mäkelä O. Immunoglobulin G subclass antibody responses in influenza A and parainfluenza type 1 virus infections. Clin Exp Immunol. 1985 Apr;60(1):130–138. [PubMed]
  • Keil W, Geyer R, Dabrowski J, Dabrowski U, Niemann H, Stirm S, Klenk HD. Carbohydrates of influenza virus. Structural elucidation of the individual glycans of the FPV hemagglutinin by two-dimensional 1H n.m.r. and methylation analysis. EMBO J. 1985 Oct;4(10):2711–2720. [PubMed]
  • Linde GA. Subclass distribution of rubella virus-specific immunoglobulin G. J Clin Microbiol. 1985 Jan;21(1):117–121. [PMC free article] [PubMed]
  • Linde GA, Hammarström L, Persson MA, Smith CI, Sundqvist VA, Wahren B. Virus-specific antibody activity of different subclasses of immunoglobulins G and A in cytomegalovirus infections. Infect Immun. 1983 Oct;42(1):237–244. [PMC free article] [PubMed]
  • Mills J, 5th, Van Kirk JE, Wright PF, Chanock RM. Experimental respiratory syncytial virus infection of adults. Possible mechanisms of resistance to infection and illness. J Immunol. 1971 Jul;107(1):123–130. [PubMed]
  • Morell A, Roth-Wicky B, Skvaril F. Immunoglobulin G subclass restriction of antibodies against hepatitis B surface antigen. Infect Immun. 1983 Feb;39(2):565–568. [PMC free article] [PubMed]
  • Niemann H, Geyer R, Klenk HD, Linder D, Stirm S, Wirth M. The carbohydrates of mouse hepatitis virus (MHV) A59: structures of the O-glycosidically linked oligosaccharides of glycoprotein E1. EMBO J. 1984 Mar;3(3):665–670. [PubMed]
  • Pabst HF, Kreth HW. Ontogeny of the immune response as a basis of childhood disease. J Pediatr. 1980 Oct;97(4):519–534. [PubMed]
  • Sarnesto A, Ranta S, Vänänen P, Mäkelä O. Proportions of Ig classes and subclasses in rubella antibodies. Scand J Immunol. 1985 Mar;21(3):275–282. [PubMed]
  • Satake M, Coligan JE, Elango N, Norrby E, Venkatesan S. Respiratory syncytial virus envelope glycoprotein (G) has a novel structure. Nucleic Acids Res. 1985 Nov 11;13(21):7795–7812. [PMC free article] [PubMed]
  • Siber GR, Schur PH, Aisenberg AC, Weitzman SA, Schiffman G. Correlation between serum IgG-2 concentrations and the antibody response to bacterial polysaccharide antigens. N Engl J Med. 1980 Jul 24;303(4):178–182. [PubMed]
  • Spiegelberg HL. Biological activities of immunoglobulins of different classes and subclasses. Adv Immunol. 1974;19(0):259–294. [PubMed]
  • Sundqvist VA, Linde A, Wahren B. Virus-specific immunoglobulin G subclasses in herpes simplex and varicella-zoster virus infections. J Clin Microbiol. 1984 Jul;20(1):94–98. [PMC free article] [PubMed]
  • Wagner DK, Clements ML, Reimer CB, Snyder M, Nelson DL, Murphy BR. Analysis of immunoglobulin G antibody responses after administration of live and inactivated influenza A vaccine indicates that nasal wash immunoglobulin G is a transudate from serum. J Clin Microbiol. 1987 Mar;25(3):559–562. [PMC free article] [PubMed]
  • Wagner DK, Graham BS, Wright PF, Walsh EE, Kim HW, Reimer CB, Nelson DL, Chanock RM, Murphy BR. Serum immunoglobulin G antibody subclass responses to respiratory syncytial virus F and G glycoproteins after primary infection. J Clin Microbiol. 1986 Aug;24(2):304–306. [PMC free article] [PubMed]
  • Walsh EE, Brandriss MW, Schlesinger JJ. Purification and characterization of the respiratory syncytial virus fusion protein. J Gen Virol. 1985 Mar;66(Pt 3):409–415. [PubMed]
  • Walsh EE, Schlesinger JJ, Brandriss MW. Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus. J Gen Virol. 1984 Apr;65(Pt 4):761–767. [PubMed]
  • Wertz GW, Collins PL, Huang Y, Gruber C, Levine S, Ball LA. Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4075–4079. [PubMed]

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