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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Cell Stem Cell. Author manuscript; available in PMC 2010 January 9.
Published in final edited form as:
PMCID: PMC2659574
NIHMSID: NIHMS91766

Tissue damage-induced intestinal stem cell division in Drosophila

SUMMARY

Stem cell division is essential for tissue integrity during growth, aging, and pathogenic assaults. Adult gastrointestinal tract encounters numerous stimulations and impaired tissue regeneration may lead to inflammatory diseases and cancer. Intestinal stem cells in adult Drosophila have recently been identified and shown to replenish the various cell types within the midgut. However, it is not known whether these intestinal stem cells can respond to environmental challenges. By feeding dextran sulfate sodium and bleomycin to flies and by expressing apoptotic proteins, we show that Drosophila intestinal stem cells can increase the rate of division in response to tissue damage. Moreover, if tissue damage results in epithelial cell loss, the newly formed enteroblasts can differentiate into mature epithelial cells. By using this newly established system of intestinal stem cell proliferation and tissue regeneration, we find that the insulin receptor signaling pathway is required for intestinal stem cell division.

INTRODUCTION

Tissue homeostasis requires the balance between removing dead cells and producing new cells. Replenishment of lost cells is likely mediated by adult stem cells resided within an individual tissue (Nystul and Spradling, 2006; Metcalfe and Ferguson, 2008; Niemeyer et al., 2006). In adult mammalian intestine, stem cells are located near the base of each crypt (Crosnier et al., 2006; Walker and Stappenbeck, 2008; Yen and Wright, 2006). These intestinal stem cells (ISC) divide to form progenitor cells in the transit amplifying zone, providing larger number of precursor cells that can replenish cells of various lineages. Wnt, BMP and Notch signaling pathways, e.g., have been shown to play important roles in mammalian intestinal cell proliferation (Fodde and Brabletz, 2007; Nakamura et al., 2007). However, specific markers for mammalian ISC remain rare, the exact identity of the stem cells is still arguable and the regulation of stem cell division in response to environmental challenge is largely unknown (Barker et al., 2007; Demidov et al., 2007; He et al., 2007; Scoville et al., 2008; Sangiorgio and Capecchi, 2008).

In adult Drosophila midgut the mature enterocytes of the entire epithelium are replaced in approximately one week (Micchelli and Perrimon, 2006). There are many ISC in Drosophila midgut and they are distributed evenly underneath the enterocytes (Micchelli and Perrimon, 2006; Ohlstein and Spradling, 2006). When an ISC divides, it gives rise to two cells, with one retaining stem cell properties and the other becomes an enteroblast. Enteroblasts are precursors that do not divide any more but can differentiate along two lineages to become enterocytes or enteroendocrine cells (see Fig. 1C). Approximately ninety percent of the enteroblasts will become enterocytes and ten percent will become enteroendocrine cells (Ohlstein and Spradling, 2007).

Figure 1
DSS feeding causes mortality and increases enteroblast number

Cell fate determination between the two daughter cells after ISC division requires Delta and Notch (Micchelli and Perrimon, 2006; Ohlstein and Spradling, 2007). Active Delta, detected as punctate cytoplasmic staining (Bray, 2006), is so far the only known specific marker for Drosophila ISC. Immediately after ISC division, higher level of active Delta is retained in the cell that remains as ISC. The newly formed, neighboring enteroblast quickly loses the active Delta. This asymmetric level of active Delta causes stimulation of the Notch signaling pathway in the newly formed enteroblast. Depending on the level of Delta in the ISC, stimulation of Notch in the enteroblast may direct the differentiation along the enterocyte lineage or the enteroendocrine cell lineage (Ohlstein and Spradling, 2007).

It is not known whether the midgut ISC have constant division cycle or can vary their division rate according to the need such as during injury or aging. To address this question, we examined whether Drosophila adult ISC division can be regulated by tissue damage. By feeding tissue damaging agents and expressing apoptotic proteins, we show that epithelial injury can increase ISC division. Furthermore, if the damage leads to epithelial cell loss, the newly divided enteroblasts can differentiate faster into mature enterocytes. These results together support the idea that epithelial damage can stimulate the underlying ISC to divide more for tissue repair. We used this newly established system to show that the insulin receptor signaling pathway is critical for ISC division.

RESULTS

Feeding of dextran sulfate sodium (DSS) causes midgut cell proliferation

When included in drinking water, DSS causes injury in the intestines of experimental mammals and complex interactions in the gut milieu lead to excessive inflammation resembling ulcerative colitis in humans (Kawada et al., 2007; Rakoff-Nahoum et al., 2004; Tlaskalova-Hogenova et al., 2005). We tested the idea that DSS could cause similar tissue damage in adult Drosophila gut. When the feeding sucrose solution contained 5% of DSS (average m.w. 40 kDa), approximately 50% of the flies died within 5 days (Fig. 1A). Together with the killing curves of 1% and 3 % DSS, we show that there is a dose dependent killing of flies by DSS. We also tested related polysaccharides and found that feeding sucrose solution containing 3% of 400 kDa DSS or dextran (a non-sulfated polysaccharide) did not change significantly the viability of the flies (Fig. 1B). Heparin, another sulfated polysaccharide, had an intermediate effect, while 5 kDa DSS also caused significantly increased lethality.

The escargot-promoter driven Gal4 expression (esg-Gal4) (Micchelli and Perrimon, 2006) when crossed with the UAS-CD8GFP can mark both ISC and enteroblasts in adult Drosophila midgut (Fig. 1C). We used these flies for feeding experiments with 3% of the various polysaccharides in the sucrose solution for 2 days. We found that the 5 and 40 kDa DSS caused a significant increase in the number of GFP positive cells, while the 400 kDa DSS and dextran did not induce a gut phenotype (Fig. 1D–G). The 40 kDa DSS is the most commonly used reagent in mammalian experiments and the effect in Drosophila gut was highly reproducible, as 84.3% (n=51) of the DSS fed samples and 0% (n=52) of control samples showed the cell proliferation phenotype. A correlation between the killing effect and cell proliferation phenotype by the various polysaccharides is also apparent.

DSS feeding increases the number of enteroblasts

The esg-Gal4/UAS-CD8GFP marks both ISC and enteroblasts. After co-staining with Delta (data not shown; Micchelli and Perrimon, 2006; Ohlstein and Spradling, 2007), we confirm that the ISC is usually the one with a lower level of GFP signal and a more rounded cell shape (Fig. 1H, arrowhead, insert). After DSS feeding most of the GFP positive cells appeared to be of bigger size with strong GFP signal (Fig. 1I). We next examined the maker Su(H)Gbe-lacZ, which is a Notch pathway target gene and is specific for enteroblasts (Micchelli and Perrimon, 2006; Ohlstein and Spradling, 2007). The staining showed that there were clearly more β-galactosidase expressing cells after DSS treatment (Fig. 1J, K).

Prospero is a homeodomain protein that is expressed specifically in enteroendocrine cells (Micchelli and Perrimon, 2006). The Prospero staining showed no apparent increase in the number of enteroendocrine cells (Fig. 1L, M). Meanwhile, punctate Delta staining in cytoplasm represents the only known ISC specific marker (Ohlstein and Spradling, 2007). In control samples, low levels of punctate Delta could be detected in cells with small nuclei, suggesting that they are ISC. In DSS treated guts, the Delta fluorescent signal became more easily detectable (Fig. 1N, O). We counted the number of cells that expressed the different markers and in each case normalized with non-stained cells based on DAPI staining. The quantification indicates that the number of cells expressing esg-Gal4/UAS-CD8GFP and Su(H)Gbe-LacZ had increased by approximately 4 fold, while Delta and Prospero positive cell number had changed by less than 1.5 fold (Fig. 1P). These results support our idea that the most prominent phenotype induced by DSS is an accumulation of enteroblasts.

Intestinal stem cell division is increased after DSS feeding

We surmised that DSS feeding caused an increase in ISC division, leading to the accumulation of enteroblasts. ISC is the only cell type in midgut that goes through division, while enteroblasts undergo endoreplication during differentiation into enterocytes, which are polyploid (Fig. 1C). Phosphorylated-histone 3 (phospho-H3) is a highly specific marker for condensed chromosomes, and thus can be used to assess the number of mitotic ISC. The number of phospho-H3 positive cells in dissected guts from DSS fed flies was approximately 20 fold higher than in control samples (Fig. 2A–C). These results support our hypothesis that DSS increases ISC division. We used 3–5 day old flies for experiments but also noted that older flies had more mitotic cells (see also Choi et al, 2008).

Figure 2
DSS increases ISC division and does not affect cell fate determination

We performed a series of experiments to determine the cell fate and stem cell properties after DSS feeding. Double staining for Delta and Su(H)Gbe-lacZ revealed that there was no overlap before or after DSS feeding (Fig. 2D–I, arrows), demonstrating that ISC and enteroblast fates are preserved. By another double staining experiment, we found that all the phospho-H3 positive cells had Delta staining (Fig. 2J–O), demonstrating that after DSS feeding ISC retained the ability to proliferate and contained high level of Delta. Therefore, the asymmetry, cell fate determination and known properties of ISC are not affected during the experimental period of DSS feeding.

We then performed the mosaic analysis with repressible cell marker (MARCM), which randomly allows Gal4 driven GFP marking of individual ISC lineage due to FLP-FRT-mediated mitotic recombination that removes the repressor Gal80 (Lee and Luo, 2001; Micchelli and Perrimon, 2006; Ohlstein and Spradling, 2006). In control guts of MARCM flies fed with sucrose, GFP expression was detected in a small number of isolated cells (Fig. 2P–R). Under the same feeding condition the DSS treated flies had more GFP positive cells and were present in clusters (Fig. 2S–U). Within each cluster only one cell exhibited punctate Delta staining (arrowhead). Each isolated cluster should represent a single lineage originated from one ISC, supporting the idea that DSS feeding increases the number of cells produced by an ISC and only one ISC is formed after each division.

DSS disrupts basement membrane organization and does not induce enteroblast differentiation

One speculation regarding stem cell-mediated repair is that stem cell division is increased and the daughter cells should differentiate to replenish lost cells. While many enteroblasts accumulated around ISC, no similar increase in mature enterocytes was observed after DSS feeding. Phalloidin stains F-actin, which is present at high levels in the apical brush border of mature enterocytes and in smooth muscle cells (Micchelli and Perrimon, 2006). In both control and DSS fed samples (Fig. 3A–F), all the MARCM GFP expressing cells (arrowheads) had smaller nuclei, stayed closer to the basal side and did not closely associate with phalloidin staining (arrows). This result suggests that DSS induces enteroblast accumulation but does not induce differentiation into mature enterocytes.

Figure 3
DSS disrupts basement membrane organization and causes enteroblast accumulation

To gain insights into the apparent lack of stimulated differentiation, we examined the tissue injury phenotype after DSS feeding. By tissue sectioning, we found that the overall morphology of the gut appeared intact and did not exhibit extensive ulcer or cell loss (Fig. 3G, H), different from that observed in mammalian experiments (see discussion). Instead, we noticed that the basal layer underneath the enterocytes appeared broken (arrows). To further investigate this phenotype, we used a fly line expressing GFP fusion protein of collagen IV (Fig. 3I), which is part of the basement membrane structure (Baba et al., 2007; LeBleu et al., 2007). Interestingly the scaffolding pattern of collagen IV-GFP became less organized in DSS fed samples (Fig. 3J). In sagittal view of control gut, there are two layers of collagenIV-GFP signal connected by transverse filaments (Fig. 3K). In DSS treated guts, the two layers became more rounded and separated (Fig. 3L). We speculate that DSS disrupts basement membrane organization, which is sensed by ISC and causes increased cell division. The lack of cell loss in the epithelial layer, however, may account for the absence of a signal required to stimulate enteroblast differentiation.

Bleomycin feeding causes damage specifically in enterocytes

We examined other tissue damaging agents in order to test the idea that epithelial cell loss may stimulate both ISC division and enteroblast differentiation, hallmarks of stem cell-mediated tissue repair. Bleomycin is an anticancer drug that is also widely used as DNA damaging agent in experimental systems (Takada et al., 2003; Morel et al., 2008). We found that feeding of bleomycin to adult flies caused lethality in a dose dependent manner (Fig. 4A). We then stained for phospho-histone 2A variant D (H2AvD), which accumulates in response to DNA damage, possibly through the protein kinase ATM (Clarkson et al., 1999; Klattenhoff et al., 2007). The control guts showed a low level nuclear punctate staining of H2AvD (Fig. 4B–D). In bleomycin fed samples, we observed a much stronger staining associated with entire nuclei of most enterocytes (Fig. 4E–G, arrow). More importantly, no H2AvD staining was observed with small nuclei in the basal side (marked by esg-Gal4/UAS-GFP, arrowhead). These results demonstrate that bleomycin feeding causes DNA damage specifically in enterocytes. We also stained the guts for Spectrin, a membrane skeleton protein localized at the apical side of enterocytes (Fig. 4H–K). The staining revealed that after bleomycin feeding the epithelial layer became disorganized and more enterocytes were shedding into the lumen (open arrow). Plastic tissue sectioning revealed a similar epithelial cell loss phenotype (Fig. 4L, M). We speculate that bleomycin may not be able to diffuse through tight junction, and thus causes damage only in enterocytes.

Figure 4
Bleomycin feeding induces damage specifically in epithelial layer

Bleomycin induces ISC division and enteroblast differentiation

We further show that bleomycin increases ISC division while the midgut cell fates are normal. Phospho-H3 staining revealed that there was a substantial, dose dependent increase in cell division (Fig. 4N). We then used the Su(H)Gbe-lacZ line to perform double staining after bleomycin feeding and observed that the Delta and β-galactosidase staining were clearly distinct (Fig. 4O, P arrowhead). Meanwhile, the MARCM experiment showed that only one Delta positive cell was present in each cluster (Fig. 4Q, R arrowhead).

One clear difference between the phenotypes induced by bleomycin versus DSS is that after bleomycin feeding there were larger cells in each cluster (Fig. 4Q, R arrow). To assess whether these bigger cells are mature enterocytes, we stained MARCM samples with fluorescent-phalloidin after bleomycin feeding. In the control guts only small cells with GFP were present (Fig. 5A–C, arrowhead). In bleomycin fed samples, there were more GFP positive cells and the bigger GFP positive cells also had tightly associated phalloidin staining at the apical side, similar to other mature enterocytes (Fig. 5D–F, arrow). In comparison, DSS induced only clusters of enteroblasts in the same period of time (Fig. 5G–I). Double feeding experiment showed that GFP-positive mature enterocytes were formed efficiently with both DSS and bleomycin in the feeding solution (Fig. 5J–L, quantification in 5M), demonstrating that DSS does not inhibit enteroblast differentiation. Overall, our data support the idea that bleomycin causes enterocyte-specific damage and cell loss, which in turn causes ISC to divide faster and facilitates enteroblast differentiation into new enterocytes.

Figure 5
Epithelial cell loss is coincident with ISC division and enteroblast differentiation

Epithelial expression of apoptotic proteins induces ISC division

We performed a series of experiment to further demonstrate that epithelial damage can indirectly induce ISC division. First, based on microarray expression assays for genes that have high expression in adult gut, we constructed an NPC (Niemann-Pick type C) 1b promoter-Gal4 driver line. Second, we examined the expression after crossing with UAS-GFP, and the GFP signal was clearly seen in the cytoplasm of large-sized enterocytes (arrow, Fig. 5.N–Q) but not in nucleus or cytoplasm of small cells (arrowhead). Thus, the NPC1b-Gal4 directs the expression only in mature enterocytes. We then expressed the viral antiapoptotic protein p35 in epithelial cells by NPC1b-Gal4 and performed bleomcyin feeding experiments. The result showed that the bleomycin-induced ISC division is suppressed by 52% (Fig. 5R).

Because p35 inhibits caspases (Domingos and Steller, 2007), our result suggests that bleomycin causes DNA damage and triggers caspase activation within the epithelial cells, and somehow stimulates ISC division. Thus, we expressed in epithelial cells the well-established apoptotic inducers Hid and Reaper, which would activate caspases. Such expression caused ISC division to increase by approximately 10 fold, based on phospho-H3 staining (Fig. 5S). Overall, our results suggest that epithelial damage by feeding tissue damaging agents or by expressing apoptotic proteins can trigger a mechanism that ultimately stimulates ISC division.

The insulin receptor signaling pathway is required for induced ISC division

We used the inducible ISC division phenotype to examine mutant fly strains and found that insulin receptor (InR) mutants showed a significantly lower number of phospho-H3 positive cells (Fig. 6A). The InR mutant also suppressed the DSS and bleomycin-induced cell organization phenotype as revealed by β-catenin staining (Fig. 6B-G), probably due to lower ISC division and smaller number of cells in the mutant guts. We examined two other components of the InR signaling pathway. Chico is the Drosophila homologue of insulin receptor substrate and the chico1 mutant showed reduced ISC division (Fig. 6A). FOXO is a transcription factor negatively regulated by insulin (Bohni et al., 1999). The FOXO transgenic construct contains point mutations at 3 phosphorylation sites that render it constitutively active equal to a loss of insulin regulation (Hwangbo et al., 2004). FOXO crossed with esg-Gal4 produced no viable adults; we therefore used the Armadillo-Gal4 driver that has low level ubiquitous expression and these fly guts showed the predicted phenotype of reduced ISC division (Fig. 6A). Thus, three components of the insulin signaling pathway are critical for ISC division.

Figure 6
Insulin receptor signaling is required for ISC division

The MARCM strategy coupled with mutant chromosomes allows positive marking of mutant cell lineage by GFP. Analysis of MARCM flies with InR mutant chromosomes revealed that after DSS feeding 90% of GFP-positive clusters contained only 1 or 2 cells (Fig. 6H, blank region of the bars, and Fig. 6I, J), demonstrating a lack of proliferation. In contrast, MARCM flies with wild type chromosomes had 80% of their GFP-positive clusters contained 3 or more cells (Fig. 6H, filled region of the bars). This demonstrates that InR function is required within ISC for proliferation.

We also used various Gal4 drivers to express a constitutively active insulin receptor (InRA1325D) to test in which cell type InR can exert its effect. The esg-Gal4 but not the NPC1b-Gal4 driver led to highly increased ISC division, suggesting that the expression of InRA1325D in precursor cells but not in epithelial cells can stimulate ISC division (Fig. 6K). Heat shock induced expression in adult flies also caused a substantial increase in ISC division, demonstrating that InR can regulate ISC division after development has completed. Furthermore, the expression of InRA1325D by the esg-Gal4 driver caused phenotypic changes in the midgut similar to that induced by DSS, including a less organized β-catenin staining pattern (Fig. 6L, M), clearly stronger Delta staining in ISC (Fig. 6N, O) and bigger clusters of esg-Gal4/GFP cells (Fig. 6P, Q). We also performed double staining of phospho-H3 and Delta in guts from flies overexpressing InRA1325D (Fig. 6R–U). 93.0% (n=499) of the phospho-H3 positive cells also contained Delta staining, demonstrating that it is the stem cells but not other cells types that have increased division. Together, the results are consistent with the idea that InR activation leads to ISC proliferation.

Regulation of ISC division by systemic insulin

Drosophila has 7 insulin-like peptides (DILPs) and when expressed by transgenic constructs all can function to change the growth and metabolic state of the animal (Brogiolo et al., 2001; Ikeya et al., 2002). The main source of DILPs is the median neurosecretory cells in the brain. Ablation of these neurosecretory cells causes insulin depletion phenotypes such as retarded growth and elevated carbohydrate level (Ikeya et al., 2002; Rulifson et al., 2002). Thus, we performed this ablation experiment by crossing a DILP2-Gal4 driver with UAS-Reaper constructs. Flies containing both DILP2-Gal4 and UAS-Reaper hatched approximately 10 days later than other flies, demonstrating that the ablation caused an insulin depletion phenotype. These flies also showed approximately 90% decrease of DSS-induced and 50% decrease of bleomycin-induced ISC division (Fig. 7A). β-catenin staining showed that these DILPs depleted flies and InR mutant flies had a similar gut phenotype, that means a better organization and smaller number of cells after DSS and bleomycin feeding (Fig. 7B–G). We examined whether this ablation experiment causes a change of gut DILP2 expression. First, we checked the DILP2-Gal4 driven UAS-GFP expression and we could not observe GFP signal in gut (data not shown). Second, we examined dilp2 mRNA expression in heads vs. guts in the ablated flies and found that the dilp2 mRNA was largely reduced in heads while the expression had no change in guts, which was very low to start with (Fig. 7H). Third, published papers also show that DILP2 has no expression in the gut (Brogiolo et al., 2001; Ikeya et al., 2002). All these suggest that the ablation experiment mainly affects brain expression. However, there are 7 DILPs in Drosophila and we cannot exclude the possibility that other gene expression is affected. Moreover, ablation of brain cells may indirectly affect gut physiology.

Figure 7
Systemic insulin level can regulate ISC division

Previous reports show that DILP2 has a consistent effect on growth and metabolism (Brogiolo et al., 2001; Ikeya et al., 2002; Rulifson et al., 2002). Thus, we expressed DILP2 in neurosecretory cells, in gut cells, or ubiquitously by DILP2-Gal4, esg-Gal4 or Armadillo-Gal4, respectively, and examined the effect on ISC proliferation. All these drivers caused approximately 2–3 fold increase in phospho-H3 positive cells in the midgut (Fig. 7I). This increase is modest but similar to previously reported 2 fold increase in overall growth (Ikeya et al., 2002). Moreover, double staining in DILP2-Gal4/UAS-DILP2 guts showed that 94.0% (n=134) of the phospho-H3 positive cells also contained Delta staining, demonstrating that it is ISC that have increased division. We conclude that the systemic level of insulin can regulate ISC division in adult midgut.

DISSCUSSION

Insulin receptor signaling is essential for cell growth, cell size control, and cell metabolism (Garofalo, 2002; Goberdhan and Wilson, 2003). The function of this pathway, however, has not been reported in any other stem cell systems. We show by various approaches that systemic and gut insulin levels can regulate ISC proliferation, likely by activating InR function within ISC. We propose a model that insulin and other signaling molecules are involved in controlling ISC proliferation and are likely required for intestinal tissue repair (Fig. 7J). Depending on whether insulin expression is increased by tissue damage, insulin may act as a permissive or instructive signal to regulate ISC proliferation.

DSS-induced colitis in small experimental mammals is a widely used model for human ulcerative colitis (Kitajima et al., 2000; Rakoff-Nahoum et al., 2004; Tlaskalova-Hogenova et al., 2005; Wirtz et al., 2007). The progression of DSS-induced colitis involves complex interactions of injured tissue with the gut milieu, including B and T lymphocytes and cytokines produced from other cells. Drosophila does not have B and T cells, but only hemocytes that function similarly to macrophages and neutrophils (Williams, 2007). Therefore, it is likely that there is no extensive inflammatory reaction in the Drosophila midgut, and this may be one of the reasons why we did not observe extensive apoptosis after DSS feeding. The tissue damage in Drosophila midgut would then be the primary effect caused by DSS without the complication of lymphocytes, thus representing a simpler model. DSS did not induce proliferation of culture cells (data not shown) and bleomycin-induced DNA damage can lead to cell cycle arrest (Hagimori et al., 2007). This suggests that ISC are indirectly stimulated by the tissue damaging effects of DSS and bleomycin.

A powerful role of stem cells in adult tissues is to divide more to help tissue repair. However, adult stem cells may have limited proliferation capacity (Radtke and Clevers, 2005; Wallenfang, 2007). In mammals, ISC division produces progenitor cells that are active in cell division to replenish the different cell types (Crosnier et al., 2006; Walker and Stappenbeck, 2008; Yen and Wright, 2006). A previous report demonstrated that after DSS feeding, mouse intestinal progenitor cells near ulcer regions had increased BrdU incorporation (Pull et al., 2005). However, it is still difficult in mammalian systems to distinguish whether those BrdU positive cells after tissue injury are only progenitor cells or also include ISC. One reason is that few stem-cell specific markers are available in mammalian systems and the exact function of those marked cells is still arguable (Barker et al., 2007; Demidov et al., 2007; He et al., 2007; Scoville et al., 2008; Sangiorgio and Capecchi, 2008). Therefore, our results establish another model system to study ISC-mediated tissue repair.

Stem cell niche is the neighboring environment that helps to maintain stem cell identity and regulates stem cell proliferation (Egger et al., 2008; Kirilly and Xie, 2007; Spradling et al., 2001; Theise, 2006; Wilson and Kotton, 2008). A previous report shows that ISC has a more extensive contact with basement membrane (Ohlstein and Spradling, 2007). Therefore, the disruption of a basement membrane component (collagen IV) by DSS may be sensed by ISC as tissue damage, or may affect the niche of ISC. It is also possible that enterocyte loss caused by bleomycin disrupts the contact and releases the inhibition to allow more ISC division. The two mechanisms of niche- and tissue damage-regulated ISC division are not mutually exclusive and can operate at the same time.

MATERIALS AND METHODS

Drosophila stocks and feeding experiments

Information on Drosophila genes and stocks is available from Flybase (http://flybase.bio.indiana.edu). y1w*, CantonS and w1118 were used as wild type stocks for gut phenotypic comparison. InRE19, InRP05545, InR93Dj-4, UAS-mCD8GFP, chico1, were obtained from Bloomington stock center; esg-Gal4 and Su(H)Gbe-lacZ were as described (Micchelli and Perrimon, 2006; Ohlstein and Spradling, 2007); collagenIV-GFP (G454) was obtained from FlyTrap collection; UAS-InRA1325D was as described (Flybase FBal0156362). The following stocks were generous gifts: UAS-hid and UAS-reaper from John Abrams, UAS-p35 from Michael Brodsky, UAS-FOXO from Marc Tatar, and DILP2Gal4 and UAS-DILPs from Ping Shen. Flies were maintained on cornmeal-yeast-molasses-agar media. Stocks were kept at room temperature. For viability tests and feeding experiments, the flies were kept at 29°C. We observed a faster appearance of the gut phenotype in this temperature; lower temperature of incubation also showed the phenotypes but took longer time. The MARCM flies were grown at 18°C and the feeding experiments with these flies were performed at 18°C. It took approximately 14 days for the tissue damage induced proliferation to occur at 18°C; higher temperature of incubation resulted in faster appearance of phenotype.

For viability tests, we consistently used 50–100 flies per vial. For gut phenotype induction, the number of flies used was 10–100 in each vial, depending on the availability of the strain. The phenotypes were reproducible regardless of the number of flies used. Feeding experiments involved using 3–5 day old flies in an empty vial containing a piece of 2.5 cm × 3.75 cm chromatography paper (Fisher). 500 ul of 5% sucrose solution was used to wet the paper as feeding medium. The chemicals included in the feeding medium were 1–5% of dextran sulfate sodium (MP Biomedicals) as indicated in the figure, 3% heparin (Sigma), 3% dextran (MP Biomedicals), 25 μg/ml bleomycin (Sigma) or as indicated in the figure. Flies that were still alive were transferred to new vials with fresh feeding media every day. Most feeding experiments were performed for two days at 29°C or specified in the figure legend.

For lineage analysis, GFP-marked intestinal stem cell clones from MARCM were generated as previously described (Lee and Luo, 2001). Briefly, flies stocks were crossed to generate offspring with the genotype: hsFLP; FRTG13 UAS-CD8GFP/FRTG13 tubulin-Gal80; tubulin Gal4/+. The final cross and offspring were maintained at 18°C. These stocks at the 18°C incubation temperature generated small number of GFP positive mitotic clones in midgut without heat shock induction of the FLP recombinase. Only flies that had all the correct chromosomes exhibited this low level of mitotic recombination and the GFP marked ISC grew to include bigger cells as observed in older flies. These are consistent with having successful mitotic recombination, which by chance eliminates the repressor Gal80 in a mitotic stem cell and allows Gal4 driven GFP to be expressed within the lineage. For tissue damage experiments, these flies were set up for feeding in 18°C for 7–14 days before dissecting guts for staining with antibody.

Immunostaining and fluorescent microscopy

For gut dissection, female flies were used routinely because of the bigger size but male flies were also used to check the phenotypes. The entire gastrointestinal tract was pulled from the posterior end directly into fixation medium containing 1X PBS and 4% Formaldehyde (Mallinckrodt chemicals). Guts were fixed in this medium for 3 hours, except for Delta staining the fixation was for 0.5 hour. Subsequent rinses, washes and incubations with primary and secondary antibodies were done in a solution containing 1X PBS, 0.5% BSA, 0.1% TritonX-100. The following anti-sera were used: anti-Delta (monoclonal 1:100 dilution), anti-Prospero (monoclonal 1:50 dilution), anti-Spectrin (monoclonal 1:100 dilution) (all from Developmental Studies Hybridoma Bank); anti-phospho-histone H3 (rabbit 1:2000 dilution) (Upstate Biotechnology); anti-βGal (monoclonal 1:500 dilution) (Promega); anti-bGal(rabbit 1:1000, pre-absorbed against fixed midguts) (Cappel, MP Biomedicals), anti-H2AvD (rabbit 1:1000 dilution) (Rockland). Secondary antibodies were used in 1:2000 dilution as follows: goat anti-mouse IgG conjugated to either Alexa 488 or Alexa 568, and goat anti-rabbit IgG conjugated to either Alexa 488 or Alexa 546 (Molecular probes). DAPI (Vectorshield, Vector Lab) was used at 0.75 mg/ml. Images were taken by a Nikon Spinning Disk confocal microscope (UMass Medical School Imaging Core Facility).

Acknowledgments

We thank Tzumin Lee for MARCM flies, Bill Theurkauf for H2AvD antibody, Ping Shen for DILP flies, Lynn Cooley for collagenIV-GFP flies originated from Bill Chia. We also thank Michael Brodsky, John Abrams, Marc Tatar, Norbert Perrimon and Allan Spradling for sharing fly stocks. The work in YTI laboratory was supported by grants from NIH (DK75545 and GM53269) and Worcester Foundation for Biomedical Research. The work in JJ laboratory was supported by grants from NIH, Welch Foundation and Leukemia and Lymphoma Society. Core resources supported by the Diabetes Endocrinology Research Center grant DK32520 were also used.

Footnotes

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References

  • Baba Y, Iyama K, Ikeda K, Ishikawa S, Hayashi N, Miyanari N, Honda Y, Sado Y, Ninomiya Y, Baba H. Differential expression of basement membrane type IV collagen alpha chains in gastric intramucosal neoplastic lesions. J Gastroenterol. 2007;42:874–880. [PubMed]
  • Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007;449:1003–1007. [PubMed]
  • Bohni R, Riesgo-Escovar J, Oldham S, Brogiolo W, Stocker H, Andruss BF, Beckingham K, Hafen E. Autonomous control of cell and organ size by CHICO, a Drosophila homolog of vertebrate IRS1-4. Cell. 1999;97:865–875. [PubMed]
  • Bray S. Notch signalling: a simple pathway becomes complex. Nature Review Molecular Cell Biology. 2006;7:678–689. [PubMed]
  • Brogiolo W, Stocker H, Ikeya T, Rintelen F, Fernandez R, Hafen E. An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control. Curr Biol. 2001;11:213–221. [PubMed]
  • Choi NH, Kim JG, Yang DJ, Kim YS, Yoo MA. Age-related changes in Drosophila midgut are associated with PVF2, a PDGF/VEGF-like growth factor. Aging Cell. 2008;7:318–334. [PMC free article] [PubMed]
  • Clarkson MJ, Wells JR, Gibson F, Saint R, Tremethick DJ. Regions of variant histone His2AvD required for Drosophila development. Nature. 1999;399:694–697. [PubMed]
  • Crosnier C, Stamataki D, Lewis J. Organizing cell renewal in the intestine: stem cells, signals and combinatorial control. Nat Rev Genet. 2006;7:349–359. [PubMed]
  • Demidov ON, Timofeev O, Lwin HN, Kek C, Appella E, Bulavin DV. Wip1 phosphatase regulates p53-dependent apoptosis of stem cells and tumorigenesis in the mouse intestine. Cell Stem Cell. 2007;1:180–190. [PubMed]
  • Domingos PM, Steller H. Pathways regulating apoptosis during patterning and development. Curr Opin Genet Dev. 2007;17:294–299. [PMC free article] [PubMed]
  • Egger B, Chell JM, Brand AH. Insights into neural stem cell biology from flies. Philos Trans R Soc Lond B Biol Sci. 2008;363:39–56. [PubMed]
  • Fodde R, Brabletz T. Wnt/beta-catenin signaling in cancer stemness and malignant behavior. Curr Opin Cell Biol. 2007;19:150–158. [PubMed]
  • Garofalo RS. Genetic analysis of insulin signaling in Drosophila. Trends Endocrinol Metab. 2002;13:156–162. [PubMed]
  • Goberdhan DC, Wilson C. The functions of insulin signaling: size isn’t everything, even in Drosophila. Differentiation. 2003;71:375–397. [PubMed]
  • Hagimori K, Fukuda T, Hasegawa Y, Omura S, Tomoda H. Fungal malformins inhibit bleomycin-induced G2 checkpoint in Jurkat cells. Biol Pharm Bull. 2007;30:1379–1383. [PubMed]
  • He XC, Yin T, Grindley JC, Tian Q, Sato T, Tao WA, Dirisina R, Porter-Westpfahl KS, Hembree M, Johnson T, et al. PTEN-deficient intestinal stem cells initiate intestinal polyposis. Nat Genet. 2007;39:189–198. [PubMed]
  • Hwangbo DS, Gershman B, Tu MP, Palmer M, Tatar M. Drosophila dFOXO controls lifespan and regulates insulin signalling in brain and fat body. Nature. 2004;429:562–566. [PubMed]
  • Ikeya T, Galic M, Belawat P, Nairz K, Hafen E. Nutrient-dependent expression of insulin-like peptides from neuroendocrine cells in the CNS contributes to growth regulation in Drosophila. Curr Biol. 2002;12:1293–1300. [PubMed]
  • Kawada M, Arihiro A, Mizoguchi E. Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease. World J Gastroenterol. 2007;13:5581–5593. [PMC free article] [PubMed]
  • Kirilly D, Xie T. The Drosophila ovary: an active stem cell community. Cell Res. 2007;17:15–25. [PubMed]
  • Kitajima S, Takuma S, Morimoto M. Histological analysis of murine colitis induced by dextran sulfate sodium of different molecular weights. Exp Anim. 2000;49:9–15. [PubMed]
  • Klattenhoff C, Bratu DP, McGinnis-Schultz N, Koppetsch BS, Cook HA, Theurkauf WE. Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. Dev Cell. 2007;12:45–55. [PubMed]
  • LeBleu VS, Macdonald B, Kalluri R. Structure and function of basement membranes. Exp Biol Med (Maywood) 2007;232:1121–1129. [PubMed]
  • Lee T, Luo L. Mosaic analysis with a repressible cell marker (MARCM) for Drosophila neural development. Trends Neurosci. 2001;24:251–254. [PubMed]
  • Metcalfe AD, Ferguson MW. Molecular and Cellular Basis of Regeneration and Tissue Repair: Skin stem and progenitor cells: using regeneration as a tissue-engineering strategy. Cell Mol Life Sci. 2008;65:24–32. [PubMed]
  • Micchelli CA, Perrimon N. Evidence that stem cells reside in the adult Drosophila midgut epithelium. Nature. 2006;439:475–479. [PubMed]
  • Morel F, Renoux M, Lachaume P, Alziari S. Bleomycin-induced double-strand breaks in mitochondrial DNA of Drosophila cells are repaired. Mutat Res. 2008;637:111–117. [PubMed]
  • Nakamura T, Tsuchiya K, Watanabe M. Crosstalk between Wnt and Notch signaling in intestinal epithelial cell fate decision. J Gastroenterol. 2007;42:705–710. [PubMed]
  • Niemeyer P, Krause U, Kasten P, Kreuz PC, Henle P, Sudkam NP, Mehlhorn A. Mesenchymal stem cell-based HLA-independent cell therapy for tissue engineering of bone and cartilage. Curr Stem Cell Res Ther. 2006;1:21–27. [PubMed]
  • Nystul TG, Spradling AC. Breaking out of the mold: diversity within adult stem cells and their niches. Curr Opin Genet Dev. 2006;16:463–468. [PubMed]
  • Ohlstein B, Spradling A. The adult Drosophila posterior midgut is maintained by pluripotent stem cells. Nature. 2006;439:470–474. [PubMed]
  • Ohlstein B, Spradling A. Multipotent Drosophila intestinal stem cells specify daughter cell fates by differential notch signaling. Science. 2007;315:988–992. [PubMed]
  • Pull SL, Doherty JM, Mills JC, Gordon JI, Stappenbeck TS. Activated macrophages are an adaptive element of the colonic epithelial progenitor niche necessary for regenerative responses to injury. Proc Natl Acad Sci U S A. 2005;102:99–104. [PubMed]
  • Radtke F, Clevers H. Self-renewal and cancer of the gut: two sides of a coin. Science. 2005;307:1904–1909. [PubMed]
  • Rakoff-Nahoum S, Paglino J, Eslami-Varzaneh F, Edberg S, Medzhitov R. Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis. Cell. 2004;118:229–241. [PubMed]
  • Rulifson EJ, Kim SK, Nusse R. Ablation of insulin-producing neurons in flies: growth and diabetic phenotypes. Science. 2002;296:1118–1120. [PubMed]
  • Sangiorgi E, Capecchi MR. Bmi1 is expressed in vivo in intestinal stem cells. Nat Genet. 2008;40:915–920. [PMC free article] [PubMed]
  • Scoville DH, Sato T, He XC, Li L. Current view: intestinal stem cells and signaling. Gastroenterology. 2008;134:849–864. [PubMed]
  • Spradling A, Drummond-Barbosa D, Kai T. Stem cells find their niche. Nature. 2001;414:98–104. [PubMed]
  • Takada S, Kelkar A, Theurkauf WE. Drosophila checkpoint kinase 2 couples centrosome function and spindle assembly to genomic integrity. Cell. 2003;113:87–99. [PubMed]
  • Theise ND. The stem cell niche and tissue biology. Stem Cell Rev. 2006;2:169–170. [PubMed]
  • Tlaskalova-Hogenova H, Tuckova L, Stepankova R, Hudcovic T, Palova-Jelinkova L, Kozakova H, Rossmann P, Sanchez D, Cinova J, Hrncir T, et al. Involvement of innate immunity in the development of inflammatory and autoimmune diseases. Ann N Y Acad Sci. 2005;1051:787–798. [PubMed]
  • Walker M, Stappenbeck T. Deciphering the ‘black box’ of the intestinal stem cell niche: taking direction from other systems. Curr Opin Gastroenterol. 2008;24:115–120. [PubMed]
  • Wallenfang MR. Aging within the Stem Cell niche. Dev Cell. 2007;13:603–604. [PubMed]
  • Williams MJ. Drosophila hemopoiesis and cellular immunity. J Immunol. 2007;178:4711–4716. [PubMed]
  • Wilson AA, Kotton DN. Another notch in stem cell biology: Drosophila intestinal stem cells and the specification of cell fates. Bioessays. 2008;30:107–109. [PubMed]
  • Wirtz S, Neufert C, Weigmann B, Neurath MF. Chemically induced mouse models of intestinal inflammation. Nat Protoc. 2007;2:541–546. [PubMed]
  • Yen TH, Wright NA. The gastrointestinal tract stem cell niche. Stem Cell Rev. 2006;2:203–212. [PubMed]