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Sex Transm Infect. 2007 June; 83(3): 237–241.
Published online 2006 November 6. doi:  10.1136/sti.2006.022970
PMCID: PMC2659104

Mycoplasma genitalium: prevalence and behavioural risk factors in the general population



Mycoplasma genitalium has been shown to cause urethritis in men and cervicitis in women and may also be a causative agent in female infertility.


To estimate the prevalence of urogenital M genitalium infection and identify sexual behavioural risk factors in the general population.


Participating individuals were 731 men and 921 women aged 21–23 years and not seeking the healthcare system because of symptoms. They answered questionnaires on sexual behaviour and provided samples for M genitalium testing.


In women aged 21–23 years, the prevalence of infection was 2.3% (21/921) and in men of the same age it was 1.1% (8/731). For both sexes, an increasing number of partners was associated with a greater chance of being infected. Among women a shorter duration of a steady relationship and having a partner with symptoms was associated with being infected, and for men younger age at first intercourse was associated with M genitalium infection.


We conclude that the prevalence of infection in the general population is too low for population‐based screening. However, the development of test algorithms based on behavioural risk factors is a promising alternative.

Mycoplasma genitalium infection has only recently been established as an infection causing urethritis in men and cervicitis in women.1 Individuals attending sexually transmitted disease (STD) clinics have been the group most thoroughly studied and those with urethritis/cervicitis have been compared with asymptomatic STD clinic attendees. Men with urethritis had M genitalium prevalences of 9.4–29.2%,2,3,4,5,6,7 whereas asymptomatic men in the same high‐risk populations had prevalences of 0.8–8.5%.2,3,4,5,6,7 The corresponding figures for women with cervicitis in two major studies were 10–11%2,8 and for women without cervicitis it was 3–5%. The concordance rate between partners is high, ranging from 45% to 58% of female partners to male index patients2,9 whereas 38% and 56% of male partners to female index patients have been shown to be infected.2,10 The optimal treatment for the infection has not yet been established, but azithromyzin has shown promising results.11

Although M genitalium is now established as a relevant sexually transmitted pathogen for symptomatic men and women, little is known about the infection in the general population. Recent research has shown that M genitalium is prevalent in women with pelvic inflammatory disease1 and acute endometritis12 and has been shown to be able to ascend the upper genital tract13 possibly by attaching to human spermatozoa.14 A sero‐epidemiological case–control study15 has found an association between antibodies against M genitalium in women having tubae factor infertility when compared with women having other kinds of infertility. Thus, several studies indicate that M genitalium might be a causative agent in female infertility, and this leads to questions on infection in the general asymptomatic population.

The aim of this study was to estimate the prevalence of M genitalium infections and to study associations with sexual behavioural risk factors for men and women in the general population.

Materials and methods

Study population

This study was performed on specimens and questionnaires obtained from a population of 21–24‐year‐old men and women participating in a population‐based screening programme on Chlamydia trachomatis in 1997–8.16 Aarhus county has a population of 631 000 inhabitants (12% of the Danish population), and the second largest city in Denmark (Aarhus city) is situated in Aarhus county. A total of 5 000 men and 4 000 women were invited to participate, and 1 033 men and 1 175 women submitted specimens to be tested for C trachomatis infection. The participating individuals were asked whether they would accept the storage of the specimen and questionnaire for future research use. A total of 731 men and 921 women accepted and were included for analysis in this study.

Specimens and storage conditions

Participants obtained the specimens by self‐collection at home and mailed them by ordinary mail directly to the laboratory. Men mailed a first‐void urine (FVU) specimen in a transport container, whereas women mailed a self‐administered vaginal pipette specimen as described previously.16 The diagnostic efficacy of this method for C trachomatis testing was established previously.17 In the laboratory, the specimens were analysed for C trachomatis (see below), the test result were mailed directly to the participating individuals, and in cases of a positive‐test result the infected individuals contacted a doctor for treatment and partner notification. Immediately after performing the C trachomatis analysis, the specimens were stored at −80°C.

Detection of C trachomatis

All C trachomatis analyses were performed on fresh samples in 1997 and 1998 at the Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark, using a transcription‐mediated amplification assay (Gen‐probe, San Diego, USA)18 according to the manufacturer's instructions. C trachomatis positive test results were confirmed.

Detection of M genitalium

In 2004 and 2005, the specimens were analysed for M genitalium infections at Statens Serum Institut, Copenhagen, Denmark. M genitalium was detected by an inhibitor‐controlled real‐time TaqMan polymerase chain reaction using primers detecting the M genitalium MgPa adhesion gene.19 All positive results were confirmed by a polymerase chain reaction detecting the MgPa adhesion gene.20


Medline was used to search for published studies describing self‐reportable predictors for C trachomatis infection, and on the basis of these studies we constructed a questionnaire. The predictor for C trachomatis infection, “vaginal douching”,21 was left out of the questionnaire, as this practice is not performed in Denmark and no Danish word exists for the procedure. Symptoms were described as vaginal discharge, dysuria, intermenstrual or irregular bleeding in women and urethral discharge or dysuria in men. The participants were asked to answer the questions by simply ticking boxes and no open questions were asked. All individuals received a questionnaire together with the C trachomatis test results from 1997 to 1998, and the tested individuals were asked to complete and return the questionnaire in a prestamped, preaddressed envelope.

Statistical analyses

The questionnaires were coded using Teleform.22 For analysis of data, the computer program STATA was used. First, the prevalence of M genitalium and C trachomatis infection with 95% confidence intervals (CI) was calculated. Then we performed a univariate logistic regression analysis with self‐reportable characteristics as independent variables and M genitalium diagnosis as the dependent variable. For continuous variables, several logistic regression analyses including different cut‐off points were analysed and the cut‐off points with the lowest p value are reported. Because of a low prevalence of M genitalium infection resulting in a low number of cases in the analysis, it was not reasonable to perform a multivariate analysis as planned.


The study was approved by the local Ethical Committee in the county of Aarhus and the Danish Data Protection Agency.


Prevalence in the general population

The prevalence of M genitalium infection was 2.3% (95% CI 1.32% to 3.24%) in women and 1.1% (95% CI 0.3% to 1.9%) in men and the corresponding prevalences of C trachomatis were 8.4% (95% CI 6.6% to 10.2%) and 5.6% (95% CI 3.9% to 7.3%) (table 11),), respectively. Only 2 of 21 M genitalium infected women had a coinfection with C trachomatis and 1 of 8 men had dual infection. Table 22 shows the key characteristics of the study population regarding sexual behaviour and social status.

Table thumbnail
Table 1 Behavioural characteristics and social status of individuals included in the study
Table thumbnail
Table 2 Prevalences of Mycoplasma genitalium and Chlamydia trachomatis infections

Risk factors for women

In the univariate analysis of risk factors associated with M genitalium in women, we found that especially increasing numbers of partners were associated with a greater risk of being infected (table 33).). Thus, women with >3 partners during the past 6 months had an OR of 3.48 (95% CI 0.74 to 16.29) of being infected compared with those having 0–1 partner; women with 4–5 partners during the past year had an OR of 19.83 (95% CI 5.01 to 78.55) compared with those having 0–1 partner; and women with >10 lifetime partners had an OR of 7.33 (95% CI 2.44 to 26.34) compared with those having 1–3 lifetime partners. Also, the duration of a steady relationship influenced the M genitalium risk for women and being in a steady relationship for more than a year significantly reduced the risk of the infection compared with those in a steady relationship for <6 months (OR = 0.13; 95% CI 0.03 to 0.52). Use of condoms during last intercourse did not significantly reduce the risk of M genitalium infection (OR = 0.45; 95% CI 0.13 to 1.55) and use of oral contraception was not associated with the presence of the infection. Having a partner with urogenital symptoms was significantly associated with the infection (OR = 2.49; 95% CI 1.08 to 6.21), but neither symptoms in the tested women nor having other sexually transmitted infections (STIs) were associated with being infected.

Table thumbnail
Table 3 Univariate analysis of possible risk factors for Mycoplasma genitalium infections

Risk factors for men

For men as in women increasing numbers of partners were associated with greater risk of being infected, and, thus, having >3 partners during the past 6 months increased the risk of M genitalium infection with an OR of 11.91 (95% CI 1.91 to 73.93) compared with those having 0–1 partner. Furthermore, men with >6 partners during the past year had an OR of 8.85 (95% CI 1.43 to 54.92) compared with those having 0–1 partner and men with >10 lifetime partners had an OR of 6.43 (95% CI 0.71 to 58.05) of being infected with M genitalium compared with those having 1–3 partners. Among men, a significant reduction in the prevalence of M genitalium could be seen if sexual debut was after 18 years compared with a sexual debut at 10–13 years (OR = 0.07; 95% CI 0.01 to 0.54). Neither contraceptive habits, symptoms nor other STIs could be shown to significantly influence the presence of M genitalium infection.


In this study, we estimated the prevalence of M genitalium infection in the general population of 21–23‐year‐old individuals and identified behavioural risk factors for the infection. Among women, the prevalence of infection was 2.3% (95% CI 1.32% to 3.24%) and the associated risk factors were an increasing number of partners during the past 6 and 12 months, a shorter duration of steady relationship and having a partner with symptoms. The prevalence of infection in men was 1.1% (95% CI 0.3% to 1.9%) and younger age at first intercourse and a higher number of partners during the past 6 and 12 months were significantly associated with being infected.

The establishment of M genitalium as a cause of sexually transmitted infections and subsequently the evidence in a seroepidemiological case–control study of an association between M genitalium antibodies and tubae factor infertility15 obviously calls for investigation of the infection in the general population. Thus, this study aims to assist in the development of evidence‐based guidelines regarding whether and how the infection should be handled in the general population. To our knowledge, studies on the prevalence in the general population have not been published and a systematic evaluation of behavioural M genitalium risk factors has only been published regarding women in a high‐risk population.8

It might be a limitation of our study that M genitalium analysis was performed on specimens that had been stored at −80°C for 7 years. This could potentially decrease the M genitalium detection rate compared with the prevalence of true infection in the population, but a recent study19 showed that freezing of male FVU at −20°C for 1–18 months did not significantly reduce sensitivity. In the same study, a decreased detection rate was observed for female FVU after freezing,19 but the vaginal pipette specimens used in our study have not been studied regarding stability of M genitalium DNA after storage. However, as the prevalence of infection among women in our study corresponds to the expected prevalence of M genitalium based on findings in other studies (see below), we believe that loss of M genitalium DNA in the stored vaginal pipette specimens is low. Another limitation of our study is related to the analysis of risk factors as the low prevalence of infection, especially among men, resulted in a low number of cases available for the multivariate analysis. However, this is among one of the first studies to evaluate risk factors in the general population and even with a higher number of cases our study would mainly constitute a hypothesis on parameters to select in future research aimed at producing test algorithms for use in the general population. In future research of risk factors in the general population, larger populations will be warranted.

Recently, an abstract was presented by Manhart et al23 showing a prevalence of M genitalium of 1.1% in men and 1.4% in women from the general American population aged 18–27 years. In other studies, different populations with an expected low risk of STIs have been defined as control groups. In a Swedish study10 none of 59 women aged 22–26 years attending a clinic for a pap‐smear were infected, in two Japanese studies pregnant women24,25 had prevalences of 0% (0/80) and 1% (1/90), and in a study from the United Kingdom26 none of 37 women aged 21–45 years undergoing tubal ligation had M genitalium. In asymptomatic women attending STD clinics, the prevalence was 3%2 and 5%.8 Thus, a prevalence of 2.3% among women in our study is within the expected range as our population consists of a mixture of high and low‐risk individuals, as is seen in the descriptive data of the population (table 11).). For males, a low prevalence of 1.1% is also in line with other studies showing a prevalence of 1.1% in men attending a Department of Medicine for check‐up27 in Japan and prevalences of 0.8% to 8.5% among asymptomatic men seen in STD clinics.2,3,4,5,6,7 The low coinfection rate with C trachomatis among both men and women is somewhat surprising, but in line with results found in other studies.2,10 On the basis of the low prevalence in the general population, a widespread screening of the population between 21 and 24 years of age will not be feasible even if M genitalium at a later stage shown to be of importance in developing infertility. Thus, the definition of risk factors and definitions of test algorithms might be of major importance in the future.

In our study, we identified several sexual behavioural risk factors significantly associated with M genitalium infection, which supports the fact that the mode of transmission is sexual. The risk factors showing the strongest association with M genitalium infections in our study were increasing numbers of partners, and this is in line with an earlier study of risk factors among women attending an STD clinic in Seattle.8 We also found that condoms were not significantly protective against M genitalium infection, and this is in line with the study from Seattle8 and with a Chinese study of condom use in commercial sex workers,28 where only an insignificant protection was found. This points to the possibility that condoms might be less protective against M genitalium infection than against other STIs such as C trachomatis.29

We conclude that the prevalence of M genitalium infection in the general population is low. However, based on our results we also hypothesise that it will be possible in the future to define test algorithms based on behavioural risk factors to select individuals with a higher prevalence of infection. With the current knowledge, it is not known whether such selective screening will be relevant to prevent urogenital and reproductive complications, and further research in long‐term complications related to M genitalium infections is urgently needed.

This study received financial support from Bangs Fond and PLU‐fonden.

Key messages

  • Mycoplasma genitalium is a sexually transmitted infection.
  • The prevalence of M genitalium is low in the general population.
  • A high‐risk population can be identified through sexual behavioural factors.
  • Selective screening for M genitalium in the general population may be a possibility in the future.


We gratefully acknowledge the high‐quality technical assistance of Birthe Dohn at Statens Serum Institute, Copenhagen, in analysing specimens for Mycobacterium genitalium. Furthermore, we thank Mette Jensen, Linda Jensen, Marlis Schlesinger and Gitte Høy at the Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark, for high‐quality technical assistance in analysing specimens for Chlamydia trachomatis.


FVU - first void urine

STD - sexually transmitted disease

STI - sexually transmitted infection


Competing interests: None declared.

Contributors: BA initiated the study, was responsible for collecting samples and questionnaire data, participated in data analysis and wrote the manuscript. IS participated in planning the study, performed the statistical analysis of data and edited the manuscript. LO participated in planning the study, participated in collecting samples and questionnaire data and edited the manuscript. JKM participated in planning the study, participated in collecting samples and questionnaire data, was responsible for testing samples for Chlamydia trachomatis and edited the manuscript. FO participated in planning the study, participated in collecting samples and questionnaire data and edited the manuscript. JSJ participated in planning the study, was responsible for analysis of samples for Mycoplasma genitalium and edited the manuscript.


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