|Home | About | Journals | Submit | Contact Us | Français|
Objective To investigate associations of dietary iron (total, haem, and non-haem), supplemental iron, and red meat with blood pressure.
Design Cross sectional epidemiological study.
Setting 17 population samples from Japan, China, the United Kingdom, and the United States participating in the international collaborative study on macro-/micronutrients and blood pressure (INTERMAP).
Participants 4680 adults aged 40-59.
Main outcome measure Average of eight blood pressure readings.
Results In multiple linear regression analyses dietary total iron and non-haem iron were consistently inversely associated with blood pressure. With adjustment for potential non-dietary and dietary confounders, dietary total iron intake higher by 4.20 mg/4.2 MJ (2 SD) was associated with −1.39 mm Hg (P<0.01) lower systolic blood pressure. Dietary non-haem iron intake higher by 4.13 mg/4.2 MJ (2 SD) was associated with −1.45 mm Hg (P<0.001) lower systolic blood pressure. Differences were smaller for diastolic blood pressure. In most models haem iron intake from food was positively, non-significantly associated with blood pressure. Iron intake from combined diet and supplements yielded smaller associations than dietary iron alone. Red meat intake was directly associated with blood pressure; 102.6 g/24 h (2 SD) higher intake was associated with 1.25 mm Hg higher systolic blood pressure. Associations between red meat and blood pressure persisted after adjustment for multiple confounders.
Conclusion Non-haem iron has a possible role in the prevention and control of adverse blood pressure levels. An unfavourable effect of red meat on blood pressure was observed. These results need confirmation including in prospective studies, clinical trials, and from experimental evidence on possible mechanisms.
Despite progress in the detection and treatment of hypertension in recent years, high blood pressure is epidemic and remains a major cause of cardiovascular morbidity and mortality worldwide.1 2 3 Multifactorial influences of diet on blood pressure include sodium intake, alcohol intake, caloric imbalance (direct), potassium intake (inverse), and possible favourable effects of minerals, vegetable protein, and omega-3 polyunsaturated fatty acids.4 5 6 7 8 9 10 11
In 1981 the “iron-heart” hypothesis was proposed to explain differences in rates of coronary heart disease between men and premenopausal women on the basis of differences in their iron stores.12 Premenopausal women were thought to lose important amounts of iron storage through menstruation. Since this initial report, several epidemiological studies and a meta-analysis have examined the role of various measures of iron in coronary heart disease and diabetes, with conflicting conclusions.12 13 14 15 16 17 18 19 20 Some studies have shown an increased risk of coronary heart disease with greater haem iron intake (originating mainly from animal sources) but not with total iron intake.14 21 The effect of non-haem iron (all other sources of iron) has not been explored, neither has consumption of iron from dietary supplements or fortified foods. Haem and non-haem iron have distinct metabolic pathways and their intestinal absorption is influenced by different nutrients, therefore they could have distinct roles in the pathophysiology of cardiovascular disease.22 23
The relation between iron and blood pressure is largely unknown. Iron is a redox active transition metal that might contribute to the production of reactive oxygen species, oxidative stress, and inflammation—all possibly adversely related to blood pressure levels.24 25 We investigated associations of dietary iron intake (total, haem, and non-haem); one major food source of haem iron, red meat; and food plus supplemental iron, with blood pressure in the cross sectional population based international collaborative study on macro-/micronutrients and blood pressure (INTERMAP).
The methods used by the international collaborative of macronutrients and blood pressure have been described in detail.26 Briefly, the study population (1996-9) comprises 2359 men and 2321 women aged 40-59 from 17 samples: Japan (four samples), the People’s Republic of China (n=3), the United Kingdom (n=2), and the United States (n=8). Data were collected cross sectionally. Each sample was representative of a defined target population; samples were included from both the community and the workforce. People were selected randomly from population lists, stratified by age and sex to give about equal numbers (65 people) in each of four 10 year age and sex groups.26 The mean participation rate was 49%. Each participant attended the research clinic on four occasions: two visits on consecutive days, a gap averaging three weeks, then two further consecutive visits.
Blood pressure was measured twice at each clinic visit with a random zero sphygmomanometer. Measurements were taken with the participant seated, after a rest of at least five minutes and with the bladder emptied. Korotkoff sounds I and V were used for systolic and diastolic blood pressures. At two visits height and weight were measured and questionnaire data were obtained on daily alcohol intake over the past seven days and on possible confounders.
At each visit a trained interviewer used the in-depth multipass 24 hour recall method to collect data on diet. This method uses open ended questions, is suited to multiple ethnicities and languages, does not require participants to be literate, and is designed to capture total dietary intake (macronutrients and micronutrients) for 24 hours.27 We therefore recorded all foods and drinks, including supplements, consumed in the previous 24 hours. Quality control measures were extensive.28 Participants gave written informed consent on their first clinic visit, before data collection.
We carried out two timed 24 hour urine collections, coinciding with the two pairs of consecutive clinic visits. Measurements taken at a central laboratory included sodium, potassium, and creatinine levels. To estimate technical error we split about 8% of local specimens before shipment to the central laboratory.26
We excluded those who did not attend all four clinic visits, those with dietary data considered unreliable, those whose energy intake from any 24 hour recall was less than 2.1 MJ or more than 21 MJ (women), or 33 MJ (men), those with two unavailable urine collections, and those whose data on other variables were incomplete or indicated violation of the protocol. Overall, we excluded 215 people. For each participant excluded we recruited another participant.
To provide comparable data on 83 nutrients across the four countries, including iron, we converted data on food and dietary supplements into nutrient intakes by using country specific food tables, updated and standardised by the Nutrition Coordinating Centre, University of Minnesota.29 We calculated nutrient intakes from only foods and from foods plus dietary supplements. For nutrients supplying energy we calculated intake as percentage millijoules and for others as intake per 4.2 MJ. We also calculated nutrients as amount per 24 hours. To estimate intake of haem and non-haem iron from dietary total iron intake we used an established formula,30 taking into account the source of iron (meat, poultry, fish v other). We used the data on food to characterise the main food groups supplying iron and to estimate each participant’s red meat intake (defined as skeletal muscle tissue of all mammals) and their consumption of beef. Measurements for each participant were averaged for blood pressure and nutrient variables across the four visits, and for urinary excretions across the two 24 hour collections.
From the mean of the four visits we estimated the reliability of iron and red meat intake for individual participants using the formula 1/[1+(ratio/4)]×100, where the ratio is the variance within a participant divided by the variance between participants, calculated separately for eight sex and country strata and pooled by weighting of each stratum specific estimate by n−1. This gives an estimate of the size of an observed regression coefficient as a percentage of the theoretical coefficient in a univariate regression analysis of blood pressure on iron, red meat, or beef.31 32
We used partial correlation to explore the associations among dietary variables, adjusted for sample, age, and sex, and pooled across countries and weighted by sample size. To examine the relations of iron intake (total, haem, non-haem, total plus supplements, all as mg/4.2 MJ) and intake of red meat or beef (grams over 24 hours, adjusted for energy intake) to systolic and diastolic blood pressure we used multiple linear regression analyses. Adjustment for possible confounders was done sequentially: for sample, age, sex, weight, height, reported special diet, use of dietary supplements, moderate or heavy physical activity (hours daily), doctor diagnosed cardiovascular disease and diabetes, and family history of hypertension (model 1); plus 24 hour urinary excretion of sodium and potassium (or urinary sodium or creatinine, potassium to creatinine ratios) and alcohol intake over 14 days (model 2); plus levels of dietary cholesterol, saturated fatty acids, and polyunsaturated fatty acids (model 3); plus intake of either animal protein, vegetable protein, dietary fibre, magnesium, phosphorus, calcium, haem iron (red meat analysis only) or non-haem iron (red meat analysis only) entered separately to avoid colinearity (models 4a-h).
To estimate the overall association we fitted regression models by country and coefficients pooled across countries, weighted by the inverse of variance. We tested heterogeneity across countries and we assessed interactions for age and sex. Because vitamin C and alcohol increase the intestinal absorption of non-haem iron we tested interactions with these two variables in models examining non-haem iron.22 23
We carried out regression analyses on four cohorts: all 4680 participants; 2238 “non-intervened” participants (not receiving a special diet; not consuming dietary supplements; no diagnosed cardiovascular disease and diabetes; not taking drugs for high blood pressure, cardiovascular disease, or diabetes—that is, exclusion of people whose characteristics might bias the relations between iron and blood pressure10 11); 3671 non-hypertensive participants (systolic blood pressure <140 mm Hg, diastolic blood pressure <90 mm Hg, not taking antihypertensive drugs); and 2038 “non-intervened” non-hypertensive participants.
Sensitivity analyses concerned the analysis of iron from food plus dietary supplements (with and without exclusion of 15 men and 44 women with a supplemental iron intake >30 mg/day, indicative of chronic iron deficiency); adjustment for total carbohydrate, starch, or total sugars (percentage millijoules); use of nutrient densities of iron (haem, non-haem, and total) adjusted for energy; use of amount per 24 hours of iron intakes (haem, non-haem, and total) adjusted for energy; and exclusion of people (predefined) with noticeable variability in nutrient intake or blood pressure. For ease of interpretation we transformed regression coefficients to represent difference in blood pressure for each two standard deviation higher intake of iron (haem, non-haem, or total), red meat, or beef. We adjusted each standard deviation for country, estimated by the mean square error from country adjusted analysis of variance.
Analyses (IT, IJB) were done with SAS version 9.1. We considered a P value <0.01 to be significant. A P value <0.05 was used for interaction terms and tests for heterogeneity across countries.
Mean systolic blood pressure ranged from 117.2 mm Hg in Japan to 121.3 mm Hg in China. Mean diastolic blood pressure ranged from 73.2 mm Hg in China to 77.3 mm Hg in the United Kingdom. Mean iron consumption was higher in the United States and China (7.8 mg/4.2 MJ for both) than in the United Kingdom and Japan (6.2 mg/4.2 MJ and 5.3 mg/4.2 MJ). Mean body mass index and energy intake were lower in Japan and China and highest in the United States. (See table 1 on bmj.com for descriptive data of each country.)
About 6% of iron intake in Western countries (United Kingdom and United States) was the haem component. In Japan and China 9% and 3% of iron intake was haem iron, with the main food source being fish (Japan 61%, China 88%). In the United Kingdom and United States meat supplied 90% and 87% of haem iron, respectively (table 11).). The main food sources of non-haem iron in Japan and China were vegetables and beans and in the United Kingdom and United States bread and cereals.
Univariate estimates of intra-individual reliability on the basis of four 24 hour recalls for each of the 4680 participants were: for total iron, observed regression coefficient 55.8% of theoretical coefficient; for haem iron, 39.8%; for non-haem iron, 59.7% (all mg/4.2 MJ); for red meat, 44.0% (g/24 h). This means, theoretically, that true associations might be larger than the observed associations—for example 1.7 (1/0.597) times larger for non-haem iron.
Haem iron intake from food was positively correlated with intake of total protein (r=0.53) and animal protein (r=0.63). Non-haem iron intake was positively correlated with intake of dietary vegetable protein (r=0.47), fibre (r=0.54), phosphorus (r=0.47), calcium (r=0.42), and magnesium (r=0.59), and 24 hour urine potassium excretion (r=0.50).
Total iron intake from food was inversely related to systolic blood pressure (table 22).). Inverse differences in diastolic blood pressure were smaller than those for systolic blood pressure. In model 3 (adjusted for multiple possible confounders including urinary sodium and potassium, alcohol intake, dietary cholesterol, saturated and polyunsaturated fatty acids) a 2 SD higher total dietary iron intake (4.2 mg/4.2 MJ) was associated with 1.39 mm Hg lower systolic blood pressure (P<0.01) and with 0.68 mm Hg lower diastolic blood pressure (P=0.01). Associations with systolic blood pressure were significant and independent of dietary fibre, vegetable protein, animal protein, and calcium (models 4a, 4b, 4e, and 4f) and were inverse but not significant with dietary magnesium or phosphorus (models 4c and 4d). The size and strength of the associations between intake of non-haem iron and blood pressure were generally stronger than those for total iron intake (table 22).
In contrast with total dietary iron intake and non-haem iron intake, haem iron intake from food was positively associated with systolic and diastolic blood pressures (table 22)) in all but two analyses; differences in blood pressure were small and non-significant and attenuated after adjustment for possible confounders.
No interactions were detected between iron intake and sex or age, or for non-haem iron between vitamin C or alcohol intake. Heterogeneity across countries was not significant.
Associations between iron intake and blood pressure in the subcohort of 2238 non-intervened participants were similar to the whole cohort (see table 2 on bmj.com). Estimated confidence intervals were wider, reflecting the small sample size. Associations of iron, non-haem iron, and haem iron intakes with blood pressure were similar after the exclusion of hypertensive participants from the entire cohort (n=3671) and from the subcohort of 2038 non-intervened participants (see tables 3 and 4 on bmj.com).
Red meat consumption varied across countries. Participants in China reported the lowest mean consumption (24 g/24 h) and had the highest prevalence (26%) of no red meat consumption on at least one of the four surveyed days. Mean red meat intake over 24 hours was 39 g in Japan, 91 g in the United Kingdom, and 76 g in the United States. Consumption of red meat was positively associated with systolic and diastolic blood pressures (table 33).). No significant heterogeneity was observed across countries. In model 3, higher red meat intake by 2 SD (103 g/24 h) was associated with 1.25 mm Hg higher systolic blood pressure (P<0.01) and 0.73 mm Hg higher diastolic blood pressure (P=0.01). Associations between red meat and blood pressure were unaffected by controlling for haem iron or animal protein intake (models 4g and 4h). Only 5% of red meat intake among participants from China was beef compared with 33% in Japan, 40% in the United Kingdom, and 66% in the United States. When beef was analysed separately, associations with blood pressure were positive but mostly smaller (table 33).). For example, in model 3, higher beef intake by 2 SD (75 g/24 h) was associated with 0.90 mm Hg higher systolic blood pressure and 0.50 mm Hg higher diastolic blood pressure. Associations between red meat or beef intake and blood pressure in previously described subcohorts—for example, in non-intervened participants (n=2238)—were similar to the whole cohort.
After further adjustments for total carbohydrates, starch, or sugars, the associations between intake of iron (total, haem, or non-haem) or red meat or beef with blood pressure were of the same size and strength as those presented in tables 22 and 33.. Participants from China did not consume any dietary supplements containing iron, except for two participants who took high doses of supplemental iron (>30 mg/24 h). Supplements containing iron were consumed by 5% of participants from Japan, 11% from the United Kingdom, and 31% from the United States. In the United States the average total iron intake increased from 7.8 mg/4.2 MJ to 9.7 mg/4.2 MJ when iron from supplements was included. In both Japan and the United Kingdom addition of supplementary iron resulted in only small increases in total iron intake (see table 1 on bmj.com). Total iron intake from diet and supplements combined was consistently inversely associated with systolic blood pressure and diastolic blood pressure; associations were weaker than those for dietary iron alone. For example, in model 3, after exclusion of 59 participants with a high (>30 mg) supplement intake, higher total iron intake by 2 SD was associated with a difference in systolic blood pressure of −0.33 mm Hg (95% confidence interval −1.10 to 0.44) and a difference in diastolic blood pressure of −0.70 mm Hg (−1.81 to 0.41). Inclusion of these participants further weakened the associations. In sensitivity analyses, red meat showed slightly stronger associations with blood pressure; associations for iron intake (total, haem, and non-haem) and blood pressure resulted in similar estimates as those in table 22.
Intake of total iron and non-haem iron are inversely related to blood pressure. Direct associations of haem iron with blood pressure were not statistically significant. Red meat consumption was positively associated with blood pressure.
Despite several studies on dietary iron and heart disease,13 14 15 16 18 20 21 the relation of a high iron diet to blood pressure is unknown. Iron is hypothesised to play a detrimental part in atherosclerotic disease through mechanisms of oxidative stress and inflammation. We, however, report an inverse relation between total dietary iron intake and blood pressure. When we separated total dietary iron into haem and non-haem components, we observed a differential effect of these two iron sources on blood pressure; non-haem iron was inversely associated with blood pressure whereas haem iron was mainly positively although not significantly associated with blood pressure.
Separating iron sources into the haem and non-haem components makes sense biologically because their mechanisms of absorption differ. The availability of non-haem iron is inhibited or enhanced by several dietary factors—for example, vitamin C and alcohol—and its bioavailability varies significantly.33 Absorption of haem iron is more complete and less regulated than that of non-haem iron.33 About 25% of haem iron compared with 2.5% of non-haem iron is absorbed in healthy people; haem iron, but not non-haem iron, correlates with serum ferittin, an indicator of total iron stores.34 Finally, gut absorption of non-haem iron is largely decreased in populations replete with iron whereas the absorption of haem iron is little affected.34
The inverse relation of non-haem iron to blood pressure, although consistent in all our multivariable analyses, may reflect correlations of non-haem iron with multiple other nutrients from vegetable foods, such as vegetable protein, phosphorus, magnesium, fibre, and calcium (correlation coefficients between 0.50 and 0.60). For example, the addition of phosphorus intake to the multivariable model reduced the size of association of non-haem iron with systolic blood pressure by 38%. To the best of our knowledge, no previous data exist on non-haem iron and blood pressure, and therefore inferences about causality are premature; our results await confirmation in other population samples, clinical trials, and experimental studies that focus on the potential biological role of non-haem iron in the regulation of blood pressure. Also, the observed differences in blood pressure associated with higher non-haem iron intake were relatively small. Our study was, however, designed to investigate small but additive contributions of several nutrients on blood pressure. Such effects, although clinically small individually, have public health relevance for the general population where a mean reduction of only 2-3 mm Hg systolic blood pressure could result in an important reduction in burden from cardiovascular disease.35
Haem iron showed direct weak associations with blood pressure. Haem iron is found mainly in red meat, poultry, and seafood; it is a major component of total iron intake in Western diets. Its association with blood pressure probably reflects the adverse relation of meat intake to blood pressure observed in the present and previous studies.36 37 38 In our study this relation persisted after controlling for multiple confounders, including fatty acids, animal protein, and haem iron; previous studies gave no such data on potential confounders.
The inclusion of dietary total iron intake from foods and supplements yielded similar, although attenuated, data on relation to blood pressure, compared with findings for food iron alone. This result may have implications, since it has been shown that with daily iron supplementation, iron absorption in healthy people was adapted by decreasing the absorption of non-haem iron (but not haem iron) from food.39 High iron stores may contribute to the risk of disease through iron catalysed formation of reactive forms of oxygen free radicals and through altered cellular function40; therefore, self supplementation of iron should be discouraged without clinical assessment of iron status, especially for populations replete with iron, such as in the United States.41
Limitations of this study include dietary assessments dependent on reporting by participants (subject to systematic and non-systematic errors), variation among food tables of different countries, and variability in daily dietary intake. All these factors are expected to produce underestimated relations of nutrients and food groups to blood pressure. To minimise these potential errors we used extensive quality control procedures, standardisation of food tables across countries, and repeated 24 hour dietary recalls and urine collections. Iron intakes estimated by the 24 hour recall method are unbiased compared with a study that used the weighed dietary intake method over 16 days.42 Although a degree of misclassification of nutrient intakes, based on four 24 hour recalls, is present (as with all dietary assessment methods), non-differential misclassification would most likely reduce observed associations between iron and blood pressure (although the opposite might be true in some cases).43 44 All data are cross sectional, thus long term influences of dietary factors on blood pressure may be underestimated. Causality cannot be inferred. Despite the comprehensive adjustment for confounders in our analyses, we cannot exclude the possibility of residual confounding by related dietary or other variables.
We found an inverse association of dietary total iron intake and non-haem iron intake with blood pressure. A weak direct association between haem iron and blood pressure was observed, probably reflecting the adverse relation of red meat consumption to blood pressure. Higher red meat intake was independently associated with higher blood pressure, including after control for fatty acids, animal protein, and haem iron consumption. These findings need to be tested in other populations, clinical trials, and experimental studies.
We thank study staff at local, national, and international centres for their efforts; a partial listing of these colleagues is given in reference 26.
Contributors: IT conceived this study, analysed the data, interpreted the results, and drafted the manuscript. IJB was also involved with data analysis and helped with interpretation of results and preparation of the manuscript. JS and PE designed the study. JS, PE, HU, LVH, QC, and LZ interpreted the results and helped in the preparation and editing of the manuscript. PE is the guarantor.
Funding: This research has been supported by grant 2-RO1-HL50490 from the US National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; by the Chicago Health Research Foundation; and by national agencies in China, Japan (Ministry of Education, Science, Sports, and Culture, grant in aid for scientific research, No 090357003), and the United Kingdom. The sponsors had no role in the design or conduct of the study; the collection, management, analysis, or interpretation of the data; or the preparation, review, or approval of the manuscript.
Competing interests: None declared.
Ethical approval: This study was approved by the institutional review board for each participating centre.
Provenance and peer review: Not commissioned; externally peer reviewed.
Cite this as: BMJ 2008;337:a258