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J Clin Microbiol. 1993 June; 31(6): 1630–1634.
PMCID: PMC265593

Subclass-specific serum antibody responses to recombinant Norwalk virus capsid antigen (rNV) in adults infected with Norwalk, Snow Mountain, or Hawaii virus.


Subclass-specific antibody responses to the Norwalk virus capsid protein in adults challenged with Norwalk, Snow Mountain, or Hawaii virus were evaluated by solid-phase enzyme immunoassay using recombinant Norwalk virus capsid antigen (rNV). Fourfold or greater serum immunoglobulin G (IgG) antibody responses to rNV were detected in 15 of 20 volunteers challenged with Norwalk virus, and serum IgA and IgM antibody responses to rNV were seen in almost all subjects who had rNV IgG responses. Serum rNV IgG antibody responses also were detected in 6 of 15 volunteers challenged with Snow Mountain virus and 2 of 12 volunteers challenged with the Hawaii virus. However, the magnitude of antibody response and the geometric mean postchallenge rNV IgG antibody titers were lower in subjects challenged with Snow Mountain or Hawaii virus, and serum IgA and IgM responses generally did not occur.

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Selected References

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  • Baron RC, Greenberg HB, Cukor G, Blacklow NR. Serological responses among teenagers after natural exposure to Norwalk virus. J Infect Dis. 1984 Oct;150(4):531–534. [PubMed]
  • Black RE, Greenberg HB, Kapikian AZ, Brown KH, Becker S. Acquisition of serum antibody to Norwalk Virus and rotavirus and relation to diarrhea in a longitudinal study of young children in rural Bangladesh. J Infect Dis. 1982 Apr;145(4):483–489. [PubMed]
  • Blacklow NR, Greenberg HB. Viral gastroenteritis. N Engl J Med. 1991 Jul 25;325(4):252–264. [PubMed]
  • Cubitt WD, Blacklow NR, Herrmann JE, Nowak NA, Nakata S, Chiba S. Antigenic relationships between human caliciviruses and Norwalk virus. J Infect Dis. 1987 Nov;156(5):806–814. [PubMed]
  • Cukor G, Nowak NA, Blacklow NR. Immunoglobulin M responses to the Norwalk virus of gastroenteritis. Infect Immun. 1982 Aug;37(2):463–468. [PMC free article] [PubMed]
  • Dolin R, Reichman RC, Roessner KD, Tralka TS, Schooley RT, Gary W, Morens D. Detection by immune electron microscopy of the Snow Mountain agent of acute viral gastroenteritis. J Infect Dis. 1982 Aug;146(2):184–189. [PubMed]
  • Dolin R, Roessner KD, Treanor JJ, Reichman RC, Phillips M, Madore HP. Radioimmunoassay for detection of the Snow Mountain Agent of viral gastroenteritis. J Med Virol. 1986 May;19(1):11–18. [PubMed]
  • Dolin R, Treanor JJ, Madore HP. Novel agents of viral enteritis in humans. J Infect Dis. 1987 Mar;155(3):365–376. [PubMed]
  • Erdman DD, Gary GW, Anderson LJ. Development and evaluation of an IgM capture enzyme immunoassay for diagnosis of recent Norwalk virus infection. J Virol Methods. 1989 Apr-May;24(1-2):57–66. [PubMed]
  • Erdman DD, Gary GW, Anderson LJ. Serum immunoglobulin A response to Norwalk virus infection. J Clin Microbiol. 1989 Jun;27(6):1417–1418. [PMC free article] [PubMed]
  • Greenberg HB, Valdesuso JR, Kalica AR, Wyatt RG, McAuliffe VJ, Kapikian AZ, Chanock RM. Proteins of Norwalk virus. J Virol. 1981 Mar;37(3):994–999. [PMC free article] [PubMed]
  • Guest C, Spitalny KC, Madore HP, Pray K, Dolin R, Herrmann JE, Blacklow NR. Foodborne Snow Mountain agent gastroenteritis in a school cafeteria. Pediatrics. 1987 Apr;79(4):559–563. [PubMed]
  • Hayashi Y, Ando T, Utagawa E, Sekine S, Okada S, Yabuuchi K, Miki T, Ohashi M. Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo. J Clin Microbiol. 1989 Aug;27(8):1728–1733. [PMC free article] [PubMed]
  • Xi JN, Graham DY, Wang KN, Estes MK. Norwalk virus genome cloning and characterization. Science. 1990 Dec 14;250(4987):1580–1583. [PubMed]
  • Jiang X, Wang M, Graham DY, Estes MK. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol. 1992 Nov;66(11):6527–6532. [PMC free article] [PubMed]
  • Johnson PC, Mathewson JJ, DuPont HL, Greenberg HB. Multiple-challenge study of host susceptibility to Norwalk gastroenteritis in US adults. J Infect Dis. 1990 Jan;161(1):18–21. [PubMed]
  • Lewis DC, Lightfoot NF, Pether JV. Solid-phase immune electron microscopy with human immunoglobulin M for serotyping of Norwalk-like viruses. J Clin Microbiol. 1988 May;26(5):938–942. [PMC free article] [PubMed]
  • Madore HP, Treanor JJ, Buja R, Dolin R. Antigenic relatedness among the Norwalk-like agents by serum antibody rises. J Med Virol. 1990 Oct;32(2):96–101. [PubMed]
  • Madore HP, Treanor JJ, Dolin R. Characterization of the Snow Mountain agent of viral gastroenteritis. J Virol. 1986 May;58(2):487–492. [PMC free article] [PubMed]
  • Madore HP, Treanor JJ, Pray KA, Dolin R. Enzyme-linked immunosorbent assays for Snow Mountain and Norwalk agents of viral gastroenteritis. J Clin Microbiol. 1986 Sep;24(3):456–459. [PMC free article] [PubMed]
  • Matsui SM, Kim JP, Greenberg HB, Su W, Sun Q, Johnson PC, DuPont HL, Oshiro LS, Reyes GR. The isolation and characterization of a Norwalk virus-specific cDNA. J Clin Invest. 1991 Apr;87(4):1456–1461. [PMC free article] [PubMed]
  • Okada S, Sekine S, Ando T, Hayashi Y, Murao M, Yabuuchi K, Miki T, Ohashi M. Antigenic characterization of small, round-structured viruses by immune electron microscopy. J Clin Microbiol. 1990 Jun;28(6):1244–1248. [PMC free article] [PubMed]
  • Parrino TA, Schreiber DS, Trier JS, Kapikian AZ, Blacklow NR. Clinical immunity in acute gastroenteritis caused by Norwalk agent. N Engl J Med. 1977 Jul 14;297(2):86–89. [PubMed]
  • Ryder RW, Singh N, Reeves WC, Kapikian AZ, Greenberg HB, Sack RB. Evidence of immunity induced by naturally acquired rotavirus and Norwalk virus infection on two remote Panamanian islands. J Infect Dis. 1985 Jan;151(1):99–105. [PubMed]
  • Thornhill TS, Wyatt RG, Kalica AR, Dolin R, Chanock RM, Kapikian AZ. Detection by immune electron microscopy of 26- to 27-nm viruslike particles associated with two family outbreaks of gastroenteritis. J Infect Dis. 1977 Jan;135(1):20–27. [PubMed]
  • Treanor J, Dolin R, Madore HP. Production of a monoclonal antibody against the Snow Mountain agent of gastroenteritis by in vitro immunization of murine spleen cells. Proc Natl Acad Sci U S A. 1988 May;85(10):3613–3617. [PubMed]
  • Treanor JJ, Madore HP, Dolin R. Development of an enzyme immunoassay for the Hawaii agent of viral gastroenteritis. J Virol Methods. 1988 Dec;22(2-3):207–214. [PubMed]
  • Truman BI, Madore HP, Menegus MA, Nitzkin JL, Dolin R. Snow Mountain agent gastroenteritis from clams. Am J Epidemiol. 1987 Sep;126(3):516–525. [PubMed]
  • Wyatt RG, Dolin R, Blacklow NR, DuPont HL, Buscho RF, Thornhill TS, Kapikian AZ, Chanock RM. Comparison of three agents of acute infectious nonbacterial gastroenteritis by cross-challenge in volunteers. J Infect Dis. 1974 Jun;129(6):709–714. [PubMed]

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