The assembly of a bipolar, microtubule spindle during mitosis is essential for accurate chromosome segregation. In animal cells, spindle formation is organized by centrosomes, organelles that contain of a pair of centrioles surrounded by pericentriolar material (PCM) that need to be duplicated exactly once every cell division cycle, in coordination with DNA replication to maintain genome stability (1
). Licensing DNA for replication is a critical regulatory step involving the Origin Recognition Complex (ORC), the first component for assembly of a pre-Replicative-Complex (pre-RC) at each origin (2
). Accumulated evidence supports roles for ORC subunits in addition to licensing DNA replication (3
). In particular, human Orc2 subunit localizes to centrosomes and depletion of Orc2 and Orc3 causes centrosome amplification in mitosis (4
Several regulators of the DNA licensing machinery have been reported to be involved in the control of both DNA and centriole duplication (5
). Both cyclin E and cyclin A, as well as Cdk2 activity are well known positive regulators of DNA replication and also promote centrosome duplication (or re-duplication) (6
). Depletion of the DNA replication licensing inhibitor Geminin causes re-duplication of both DNA and centrosomes in human cells (12
In a screen using short interfering RNA (siRNA) for human ORC proteins with roles in centrosome biology, we found that depletion of the largest human ORC subunit, HsOrc1, leads to excess centrosomes (fig. S1
, ). Orc1 siRNA-treated U2OS cells were analyzed for centrosome defects by dual-color indirect immunofluorescence (IF) using anti-centrin 2 (stains centrioles) and anti--tubulin (stains centrosomes) antibodies. 72 hr post siRNA, 39.77 ± 3.5% of cells transfected with Orc1-1 siRNA and 25.53 ± 0.3% of cells transfected with Orc1-2 siRNA showed multiple centrosomes and centrioles, in comparison to 2.4 ± 1.2% of control cells (fig. S1C
). In Orc1-1 treated cells 33.29 ± 2.6% of the multiple centrosomes were separated rather than linked and the re-duplication occurred in different human cell lines (fig. S1 and S2
Orc1 depletion causes centriole and centrosome re-duplication
To study the kinetics of centriole and centrosome re-duplication after Orc1 depletion, U2OS cells were synchronized in mitosis by nocodazole treatment and released into the next cycle. Centriole and centrosome numbers were assessed 12 hr, 36 hr and 60 hr after nocodazole release (, fig. S3
). Disorganized disengagement of duplicated centrioles (2Pairs - Dis.) was observed 12 hr after release when most of the cells were in G1 or early S phase (, fig S3
; centrin 2). This was followed by an increase in the percentage of cells showing centriole re-duplication (>2P). The complete centrosome structure, as evidenced by -tubulin staining, was observed later, mostly 60 hr after release (-tubulin). The very early effect of Orc1 depletion on centriole disengagement and re-duplication suggests that Orc1 might have a more direct effect on these processes, whereas complete centrosome formation follows centriole re-duplication. Similar dynamics of centriole re-duplication after Orc1 depletion were observed in HeLa cells (fig. S4
). It has been proposed that centriole engagement blocks re-duplication (13
). In Orc1 depleted cells, however, the duplicated centrioles were found disengaged and underwent re-duplication, suggesting that Orc1 may block re-duplication by participating in processes of centriole engagement of the newly duplicated centrioles.
Different mechanisms for centrosome amplification have already been reported. Apparent multiple centrosomes can arise by centrosome fragmentation, centriole splitting that occurs during early mitosis after DNA damage (14
) or authentic re-duplication. Analysis of multiple parameters including DNA content (fig. S1B
), cell cycle markers (fig. S5
), centriolin staining of mature centrosomes (fig. S6 and S7
) and the numbers of centrioles per PCM () all indicate that Orc1 depletion causes bona fide
centriole and centrosome re-duplication in U2OS cells (see supplemental text
To address whether over-expression of Orc1 would affect centrosome re-duplication caused by prolonged S-phase arrest in the presence of hydroxyurea (HU) (9
), U2OS cells were transiently transfected with YFP-Orc1WT
, YFP-Orc2 or a vector carrying only YFP in the presence of HU (; fig. S8
). Centrosome re-duplication after HU treatment, but not normal duplication, was substantially inhibited in YFP-Orc1WT
cells, whereas YFP or YFP-Orc2 did not have any effect, suggesting a role for Orc1 as an inhibitor of centrosome re-duplication.
Over-expression of Orc1 blocks centrosome re-duplication
YFP-Orc1 associated with centrosomes in addition to its nuclear localization in approximately 35% of transfected cells (fig. S9A and Video 1
). Furthermore, endogenous Orc1 and other ORC subunits co-fractionated with purified centrosomes (fig. S9B
). Transiently expressed Orc1 fused to the PACT domain, a conserved motif that targets proteins to centrosomes (16
), resulted in inhibition of centrosome re-duplication in HU-treated U2OS cells (), suggesting that Orc1 controls centrosome copy number when located at centrosomes.
Centrosome re-duplication was significantly inhibited by the full length and N terminal region of Orc1 (aa 1 to 500; Orc1WT
. Nterm. Flag; ), the latter of which is deficient ORC assembly (17
). Both Orc1 versions also inhibited centrosome re-duplication when expressed after the application of the HU block, indicating that the inhibition was not caused by a G1 arrest in these cells (fig. S10
). Thus Orc1 control of centrosome copy-number is separate from its DNA replication role, does not require assembly of into ORC, but such assembly may facilitate a more efficient block.
Orc1 depletion in synchronized U2OS cells did not trigger early responses of DNA damage checkpoint pathways that are known to affect centriole and centrosome copy number (18
). Activation of Chk1 by phosphorylation on serine 317 was not detected and p27 and the p53 pathways were activated well after centriole amplification was initiated (, fig. S11
Several reports have shown that Cyclin E over-expression in mammalian cells is linked to genome and centrosome abnormalities (20
) and that centrosome re-duplication requires Cdk2 activity (7
). Elevated levels of Cyclin E were identified as early as 12hr after release of synchronized Orc1-depleted U2OS cells in Cyclin A negative cells (, also see fig. S5A and S5B
) and continued to increase. We thus hypothesized that Orc1 control of centriole and centrosome re-duplication involved Cyclin E and Cdk2. The Cdk2 inhibitor roscovitine suppressed centriole and centrosome re-duplication in Orc1 depleted cells (fig. S12
). In a second experiment, simultaneous depletion of Orc1 and Cyclin E blocked centriole and centrosome re-duplication, whereas normal duplicated centriole and centrosome numbers were observed (, fig. S13A
). Cyclin E siRNA did not change the number of cells with a 4C DNA content after Orc1-1 treatment (fig. S13B
), implying that the block of centrosome over-duplication in Orc1-1 depleted cells by Cyclin E siRNA was not due to a compete G1 arrest. In contrast, Cyclin A siRNA did not abolish centrosome re-duplication in Orc1 depleted cells ().
Orc1 depletion causes Cdk2 and Cyclin E-dependent centriole and centrosome re-duplication
We tested the ability of elevated levels of cyclins to cause centrosome re-duplication in HU treated cells expressing YFP. Orc1WT (). Co-expression of Cyclin E with YFP. Orc1WT blocked YFP. Orc1WT inhibition of HU-induced centrosome re-duplication, whereas neither Cyclin A nor Cyclin B had any effect (). HU treated cells co-expressing YFP. Orc1WT with Cyclin A showed a cytoplasmic pool of over-expressed YFP. Orc1WT in addition to the nuclear localization and an enhancement of YFP-Orc1 located at centrosomes (; the number of YFP-Orc1 positive centrosomes in the presence of Cyclin A increased six-fold compared to YFP-Orc1 alone). Co-expression of Cyclin E with YFP. Orc1WT. PACT also eliminated the ability of YFP. Orc1WT. PACT to block centrosome re-duplication, whereas Cyclin A or Cyclin B had no effect (). Altogether, the results suggest that Orc1 might directly regulate centriole and centrosome copy number in cells, and the mechanism involves Cyclin E.
Cyclin E suppresses Orc1 inhibition of centrosome re-duplication in S-phase-arrested cells
Orc1 co-precipitated with Cyclin E and Cyclin A, as previously shown (22
), whereas no association was observed with Cyclin B (). Association in a complex with Cyclin E was stronger than with Cyclin A; however, so far we have not been able to find evidence that Orc1 binds Cyclin E directly. Nevertheless, purified wild type Orc1 inhibited Cyclin E-Cdk2 and Cyclin A-Cdk2 kinase activity using histone H1 as substrate (fig. S14B
The Orc1 ‘RXL’ mutant (K235A-L237A, Orc1A-A
) impairs the direct interaction with Cyclin A, but does not affect Cyclin E binding (; fig. S14A
) and cannot block Cyclin A associated kinase activity (fig. S14B
). Although some inhibition was observed, Orc1A-A
Nterm did not block centrosome re-duplication as efficiently as wild type Orc1, suggesting that Orc1-Cyclin A interaction is required for optimal centrosome copy number control (). Orc1A-A
. PACT blocked centrosome re-duplication to the same extent as Orc1WT
. PACT (). Since Cyclin A function has been previously linked to the cytoplasmic localization of Orc1 (23
), possibly Orc1A-A
Nterm mutants were not exported efficiently to centrosomes to block HU-induced re-duplication, whereas Orc1A-A
. PACT was directly targeted to centrosomes. Accordingly, Cyclin A stimulation of Orc1 association with centrosomes was significantly reduced when co-expressed with the ‘RXL’ Orc1A-A
mutant . Altogether, the data indicates that both Cyclin E and Cyclin A play a role in Orc1 mediated control of centrosome numbers.
ORC in human cells is dynamically assembled and disassembled during the cell cycle and participates in many aspects of chromosome duplication and segregation, including functions at kinetochores, centromeres and heterochromatin (3
). Orc2 is localized to centrosomes, but its depletion affects centrosome copy number in mitosis in addition to controlling DNA replication, centromere activity and chromosome structure (4
). In contrast, Orc1 controls Cyclin E-dependent centrosome copy number by directly preventing re-duplication of centrioles immediately after centrioles duplicate upon commitment to cell division. Without Orc1, duplicated centrioles are disengaged and extensive Cyclin E-dependent centriole re-duplication occurs. Note that in the absence of Orc1 and Cyclin E, centrioles duplicate, but they do not re-duplicate, suggesting that such centriole duplication is Cyclin A dependent, consistent with previous studies (24
). But in the absence of Cyclin E and Orc1, centrosome re-duplication does not occur. Similarly, Cyclin E, but not Cyclin A over-rides the Orc1 inhibition of centrosome-re-duplication.
Cyclin E is required for centrosome duplication and re-duplication and co-precipitates with Orc1 (7
). We have not been able to detect direct binding between Cyclin E and Orc1, suggesting that a mediator protein may facilitate the binding. The mediator may be another DNA replication protein implicated in centrosome biology (12
). Furthermore, depletion of Orc1 increases Cyclin E protein levels in cells that may contribute to centriole re-duplication. Both proteins are degraded by SCF, which localizes to centrosomes and has also been implicated in control of centrosome duplication (29
There is a short time during the cell division cycle after centrioles have duplicated when Cyclin E is still present in cells (late G1 and early S phase). During this time, if Cyclin E activity is not checked, centriole re-duplication might occur. We suggest that a mechanism involving Cyclin A-dependent localization of Orc1 to centrosomes that prevents Cyclin E-dependent centriole re-duplication during this time window (fig. S15
). In this manner, Orc1 plays a dual role in coordinating DNA replication and centrosome copy number.