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J Clin Microbiol. 1992 June; 30(6): 1457–1461.
PMCID: PMC265310

Detection of borreliacidal antibodies in hamsters by using flow cytometry.


Flow cytometry can be used to detect antibody that kills Borrelia burgdorferi. Borreliacidal activity was detected within 3 h of incubating B. burgdorferi with immune serum and complement. Right-angle light scatter and propidium iodide fluorescence were the cytometric parameters which correlated best with in vitro killing of B. burgdorferi. Flow cytometry is a rapid method for determining the presence of borreliacidal activity and may lead to a better serodiagnostic test for the detection of Lyme disease.

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Selected References

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  • Barbour AG, Hayes SF. Biology of Borrelia species. Microbiol Rev. 1986 Dec;50(4):381–400. [PMC free article] [PubMed]
  • Berardi VP, Weeks KE, Steere AC. Serodiagnosis of early Lyme disease: analysis of IgM and IgG antibody responses by using an antibody-capture enzyme immunoassay. J Infect Dis. 1988 Oct;158(4):754–760. [PubMed]
  • Callister SM, Schell RF, Lovrich SD. Lyme disease assay which detects killed Borrelia burgdorferi. J Clin Microbiol. 1991 Sep;29(9):1773–1776. [PMC free article] [PubMed]
  • Coleman JL, Benach JL. Isolation of antigenic components from the Lyme disease spirochete: their role in early diagnosis. J Infect Dis. 1987 Apr;155(4):756–765. [PubMed]
  • Craft JE, Grodzicki RL, Steere AC. Antibody response in Lyme disease: evaluation of diagnostic tests. J Infect Dis. 1984 May;149(5):789–795. [PubMed]
  • Grodzicki RL, Steere AC. Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease. J Infect Dis. 1988 Apr;157(4):790–797. [PubMed]
  • Hansen K, Asbrink E. Serodiagnosis of erythema migrans and acrodermatitis chronica atrophicans by the Borrelia burgdorferi flagellum enzyme-linked immunosorbent assay. J Clin Microbiol. 1989 Mar;27(3):545–551. [PMC free article] [PubMed]
  • Hansen K, Hindersson P, Pedersen NS. Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease. J Clin Microbiol. 1988 Feb;26(2):338–346. [PMC free article] [PubMed]
  • Hechemy KE, Harris HL, Wethers JA, Stevens RW, Stock BR, Reilly AA, Benach JL. Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi. J Clin Microbiol. 1989 Aug;27(8):1854–1858. [PMC free article] [PubMed]
  • Hunter EF, Russell H, Farshy CE, Sampson JS, Larsen SA. Evaluation of sera from patients with Lyme disease in the fluorescent treponemal antibody-absorption test for syphilis. Sex Transm Dis. 1986 Oct-Dec;13(4):232–236. [PubMed]
  • Kochi SK, Johnson RC. Role of immunoglobulin G in killing of Borrelia burgdorferi by the classical complement pathway. Infect Immun. 1988 Feb;56(2):314–321. [PMC free article] [PubMed]
  • Lovrich SD, Callister SM, Schmitz JL, Alder JD, Schell RF. Borreliacidal activity of sera from hamsters infected with the Lyme disease spirochete. Infect Immun. 1991 Aug;59(8):2522–2528. [PMC free article] [PubMed]
  • Magnarelli LA, Anderson JF. Enzyme-linked immunosorbent assays for the detection of class-specific immunoglobulins to Borrelia burgdorferi. Am J Epidemiol. 1988 Apr;127(4):818–825. [PubMed]
  • Muhlemann MF, Wright DJ, Black C. Serology of Lyme disease. Lancet. 1986 Mar 8;1(8480):553–554. [PubMed]
  • Pennell DR, Wand PJ, Schell RF. Evaluation of a quantitative fluorescence immunoassay (FIAX) for detection of serum antibody to Borrelia burgdorferi. J Clin Microbiol. 1987 Nov;25(11):2218–2220. [PMC free article] [PubMed]
  • Russell H, Sampson JS, Schmid GP, Wilkinson HW, Plikaytis B. Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J Infect Dis. 1984 Mar;149(3):465–470. [PubMed]
  • Schmitz JL, Schell RF, Hejka AG, England DM. Passive immunization prevents induction of Lyme arthritis in LSH hamsters. Infect Immun. 1990 Jan;58(1):144–148. [PMC free article] [PubMed]
  • Schmitz JL, Schell RF, Lovrich SD, Callister SM, Coe JE. Characterization of the protective antibody response to Borrelia burgdorferi in experimentally infected LSH hamsters. Infect Immun. 1991 Jun;59(6):1916–1921. [PMC free article] [PubMed]
  • Steere AC. Lyme disease. N Engl J Med. 1989 Aug 31;321(9):586–596. [PubMed]
  • Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE. The spirochetal etiology of Lyme disease. N Engl J Med. 1983 Mar 31;308(13):733–740. [PubMed]
  • Wilske B, Preac-Mursic V, Schierz G, Kühbeck R, Barbour AG, Kramer M. Antigenic variability of Borrelia burgdorferi. Ann N Y Acad Sci. 1988;539:126–143. [PubMed]
  • Wilske B, Schierz G, Preac-Mursic V, Weber K, Pfister HW, Einhäupl K. Serological diagnosis of erythema migrans disease and related disorders. Infection. 1984 Sep-Oct;12(5):331–337. [PubMed]

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