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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nature. Author manuscript; available in PMC Apr 9, 2009.
Published in final edited form as:
PMCID: PMC2652134
NIHMSID: NIHMS67294
SAP-controlled T-B cell interactions underlie germinal centre formation
Hai Qi,1* Jennifer L. Cannons,2* Frederick Klauschen,1 Pamela L. Schwartzberg,2# and Ronald N. Germain1#
1 Lymphocyte Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases National Institutes of Health, Bethesda, MD 20892, USA
2 Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Correspondence and requests for materials should be addressed to R.N.G (rgermain/at/niaid.nih.gov) or P.L.S. (pams/at/nhgri.nih.gov)
*These authors contributed equally to this work.
#These authors contributed equally to this work.
Generation of long-term antibody-mediated immunity depends on the germinal centre (GC) reaction, which requires cooperation between antigen-specific T and B lymphocytes. In the human X-linked lymphoproliferative disease and its gene-targeted mouse model, loss-of-function mutations in signalling lymphocyte activation molecule-associated protein (SAP, encoded by SH2D1a) cause a profound defect in GC formation by an as yet unknown mechanism. Using two-photon intravital imaging, here we show that SAP deficiency selectively impairs the ability of CD4+ T cells to stably interact with cognate B cells but not antigen-presenting dendritic cells. This selective defect results in a failure of antigen-specific B cells to receive adequate levels of contact-dependent T cell help to expand normally, despite sap−/− T cells exhibiting the known characteristics of otherwise competent helper T cells. Furthermore, lack of stable interactions with B cells renders sap−/− T cells unable to be efficiently recruited to and retained in a nascent GC to sustain the GC reaction. These results offer a compelling explanation for the GC defect due to SAP deficiency and provide novel insights into the bi-directional communication between cognate T and B cells in vivo.
The germinal centre (GC) reaction, which supports antibody affinity maturation and the generation of B cell memory13, requires activation of CD4+ T cells by antigen-bearing dendritic cells (DCs), followed by differentiation of these T cells and their physical interactions with antigen-activated B cells4. The activated T cells promote the survival, proliferation, and differentiation of the B cells by delivering contact-dependent helper signals in an antigen-specific fashion58. Follicular helper T (TFH) cells, a subset of activated CD4+ T cells that highly express CXCR5, CD40L, ICOS, and signalling lymphocyte activation molecule-associated protein (SAP), are particularly important for a sustained GC response9.
In human X-linked lymphoproliferative disease (XLP) and its gene-targeted mouse model, lack of functional SAP protein in T cells causes a profound defect in GC formation and humoral immunity1017, but how SAP regulates the GC-promoting T helper cell response is unknown. An intracellular adaptor protein, SAP binds to signalling lymphocyte activation molecule (SLAM) and other transmembrane molecules of the SLAM family18,19. It modulates T cell antigen receptor signalling and promotes type 2 T helper (TH2) cell differentiation13,14,20. However, impaired TH2 differentiation alone is not an adequate explanation for the profound GC defect seen in SAP-deficient animals17,21,22. To better understand how SAP deficiency results in impaired GC formation, we took a combined approach of classical cellular immunology and intravital 2-photon imaging, which allowed us to examine not only the activation phenotype of T cells but also the dynamics of their critical interactions with DCs and B cells in vivo23.
Because optimal T cell activation and differentiation in vivo require long-lasting T-DC conjugation2427, we first examined the impact of SAP deficiency on T-DC interactions using OT-2 TCR transgenic T cells specific to I-Ab complexed with an ovalbumin-derived peptide (OVA323). Behaviours of sap−/− and sap+/+ T cells in the same draining lymph node (LN) were compared following co-transfer into mice previously injected s.c. with OVA323-pulsed DCs. Sap−/− and sap+/+ OT-2 T cells showed equivalent contact times with DCs presenting OVA323 (Figs. 1a and 1b, Supplementary Movie 1). Consistent with this result, sap−/− and sap+/+ OT-2 T cells proliferated and accumulated to a similar extent (Fig. 1c). Within the expanded pool of activated T cells of either genotype, similar frequencies of CXCR5+ICOS+ TFH precursors were observed and both groups of T cells migrated into the B cell follicular areas of the LN (Fig. 1d and 1e). These data indicate SAP deficiency does not grossly alter initial T cell interaction with or activation by antigen-presenting DCs in vivo.
Figure 1
Figure 1
Sap−/− T cells normally interact with and are activated by DCs in vivo
During a T-dependent B cell response, helper T cells activated by DCs must subsequently engage antigen-triggered B cells based on class II major histocompatibility complex (MHC)-restricted antigen recognition7,28. This process is characterized by long-lasting, mobile conjugate pairs formed between cognate T and B cells29. Based on these prior observations, we used intravital imaging to assess whether SAP deficiency affects antigen-dependent T-B interactions in vivo. Sap−/− and sap+/+ OT-2 T cells were co-transferred into mice together with wild-type B cells expressing the MD4 transgenic B cell receptor, which recognizes hen egg lysozyme (HEL). As antigen, cross-linked conjugates of HEL and OVA (HEL-OVA) were used to allow activation of both T and B transgenic cells and to permit MHC class II-dependent antigen presentation to the OT-2 T cells by the MD4 B cells. Following immunization, sap+/+ OT-2 T cells engaged migrating MD4 B cells and formed long-lasting mobile conjugate pairs, while sap−/− OT-2 T cells showed predominantly brief contacts with the same cohort of MD4 B cells (Figs. 2a and 2b; Supplementary Movies 2 and 3; mean±SEM of the median T-B contact time by sap+/+ vs. sap−/− T cells: 19.2±2.6 vs. 8.2±1.9 minutes, 5 experiments). Thus, are in striking contrast to the lack of significant effects on the duration of T-DC interactions (Fig. 1a and 1b), the absence of SAP expression in T cells severely reduces the longevity of T-B interactions in vivo.
Figure 2
Figure 2
Sap−/− T cells are defective in adhesion to cognate B cells
To examine more quantitatively the differential effect of SAP deficiency on T-APC interactions, a flow cytometry-based in vitro assay was utilized in which shear stress was imposed on unfixed conjugates between activated OT-2 T cell blasts and OVA323-pulsed DCs or activated B cells. While a dose-dependent increase in T-B conjugate efficiency was observed with sap+/+ OT-2 T cells, conjugate formation by sap−/− T cells was low at all peptide concentrations tested (Fig. 2c). In contrast, within the same non-saturating peptide dose range, sap−/− T cells were as efficient as wild-type T cells in forming conjugates with DCs (Fig. 2d). To determine whether the defective adhesion to B cells by activated sap−/− T cells might have been programmed during their priming interactions with DCs and whether SAP expression is specifically required during T-B interactions, DNA constructs encoding either green fluorescent protein (GFP) or a GFP-SAP fusion protein were independently transfected into sap−/− T cell blasts. Complementation with GFP-SAP but not GFP alone fully rescued T-B conjugate formation by transfected sap−/−T cell blasts (Fig. 2e; also see Supplementary Fig. 1). Thus, the requirement for SAP expression in T cells for optimal adhesion to B cells is contemporaneous with T-B interaction. SAP facilitates the recruitment of Fyn kinase to cytoplasmic tails of SLAM-related proteins, which is important for SAP regulation of Th2 cytokine production20,30 but less so for SAP-controlled GC development17,31. Consistent with these latter results, we found that SAPR78A, a mutant SAP capable of binding SLAM-related molecules but severely impaired in binding Fyn kinase20,30, rescued T-B conjugate formation in vitro (Supplementary Fig. 2). Taken together, our analyses of T cell-APC conjugation establish that SAP critically regulates the contact duration and the adhesive strength of cognate T-B but not antigen-driven T-DC interactions.
The cell numbers used in the preceding experiments can result in competition among T cells for antigen on presenting cells in vivo32, raising the question of whether sap−/− T cells would, if present alone, form more stable contacts with antigen-specific B cells or provide B cells with a summed contact time from sequential brief interactions comparable to the total interaction time with wild-type T cells. To examine these issues, a small number (6×104) of sap+/+ or sap−/− OT-2 T cells were separately transferred into recipient B6 mice that concomitantly received 3×105 MD4 B cells. Cumulative interactions between B and T cells were assessed by continuous intravital imaging over a 3-hour period of time between 60 to 72 hours post- immunization, when substantial clonal expansion has begun and physical co-localization of activated T and B cells can be observed 8,29,33,34. Under these modified conditions, sap−/− OT-2 cells still demonstrated abbreviated contacts with activated MD4 B cells following immunization with HEL-OVA (2.1±0.2 minutes [mean±SEM] vs. 8.9±0.8 minutes for sap+/+ T cells, p<0.0001; Fig. 3a, Supplementary Movie 4). While many contacts involving sap−/− T cells were brief and similar to non-cognate T-B contacts in duration, on the population level they lasted for a statistically longer period of time (2.1±0.2 vs. 1.3±0.1 minutes for non-cognate interactions, p=0.0014), indicating that antigen-specific interactions occurred between sap−/− OT-2 cells and MD4 B cells but were not sustained. Individual B cells were also tracked for their interactions with different T cells. MD4 cells often accumulated >1 hour of interactions with sap+/+ OT-2 T cells, while they were rarely able to accumulate more than 20 minutes of contact with sap−/− OT-2 T cells over the same period (Figs. 3b–d; Supplementary Movies 4 and 5). B cells remained equally trackable under the two conditions and interacted with similar numbers of sap−/− or sap+/+ T cells (Supplementary Fig. 3). The severe reduction in cumulative time of contact with sap−/− OT-2 T cells correlated with a pronounced impairment of MD4 B cell clonal expansion (Fig. 3e). Nonetheless, sap−/− OT-2 T cells expanded comparably to their sap+/+ counterparts and expressed similar levels of CD40L, ICOS, OX40, and CXCR5 (Fig. 3f; Supplementary Fig. 4) at this time. The latter is a phenotype considered characteristic of activated T cells capable of effectively promoting humoral responses and with features of TFH precursor cells as commonly defined9,35. In concert with the previous data, these findings suggest that it is not an intrinsic inability of SAP-deficient T cells to express key molecular signals, but rather a failure to exchange these signals during sufficiently long periods of contact with B cells that accounts for their markedly reduced capacity to promote B cell expansion and subsequent GC development.
Figure 3
Figure 3
B cells fail to receive contact-dependent help from SAP-deficient T cells
Recruitment of helper T cells to a forming GC is necessary for maintaining the GC reaction36, and GC-localized TFH cells are required for effective immunoglobulin class-switching and antibody affinity maturation9. To explore whether SAP deficiency also impairs T cell recruitment to and retention within nascent GCs, we transferred equal numbers (3×104) of CFP-expressing sap+/+ OT-2 cells and GFP-expressing sap−/− OT-2 cells into B6 mice together with 3×105 non-fluorescent MD4 cells. Six to 8 days after HEL-OVA immunization, GCs developed within the draining LN, seen as GL7+ areas that largely excluded IgD+ naïve B cells (Supplementary Fig. 5). While the IgD+ follicular mantle zone was populated by both sap+/+ and sap−/− OT-2 cells, the GC area predominantly contained sap+/+ OT-2 cells, consistent with a failure of GC recruitment and/or retention of sap−/− T cells.
To examine this issue dynamically, recipient mice were also given dye-labelled naïve B cells 24 to 48 hours before intravital imaging to differentiate follicular mantle and GC areas. Although individual naïve B cells migrate in and out of GCs37, as a population they remain substantially excluded from GCs (Supplementary Fig. 5), allowing simultaneous identification of the follicular area and approximation of the GC-mantle border using time-averaged images (see Supplementary Fig. 6 for details). Sap+/+ and sap−/− OT-2 T cells exhibited strikingly different dynamic patterns within the follicle (Supplementary Movie 6 and Fig. 4a). While sap+/+ OT-2 T cells migrated freely into and accumulated within the GCs, sap−/− OT-2 T cells mainly swarmed in the follicular mantle. To quantify migratory behaviours of the two types of T cells around GCs, a tessellation algorithm was used to retrieve the GC surface (Supplementary Fig. 6) and define a virtual mantle-GC interface zone was then defined as encompassing spatial coordinates within 10 μm of the GC outer surface. T cell tracks were subsequently classified according to their interactions with this interface zone (Fig. 4b and 4c). When reaching the interface from the follicular mantle, sap+/+ OT-2 T cells were more likely to continue into the GC than to return to the mantle. Sap−/− T cells exhibited the opposite behaviour, being more likely to turn back than to cross into the GC. Conversely, when reaching the interface zone from within the GC, sap+/+ T cells were more likely to return to the GC than to escape into the mantle, whereas sap−/− T cells were much more likely to escape than to return. For those tracks that started from the interface zone, sap−/− T cells exhibited a preference for migrating into the mantle as compared to moving into the GC, while for sap+/+ T cells the movement in these two directions was comparable. Consistent with these distinct migration patterns, sap−/− T cells also exhibited significantly shorter GC retention times (Supplementary Fig. 7). Therefore, in the absence of SAP, T cells are not efficiently recruited into or retained within a nascent GC. These data suggest SAP-deficient T cells cannot act as effective GC-associated TFH cells to sustain the GC reaction, a defect that would contribute to the profound impairment of GC responses in SAP-deficient hosts.
Figure 4
Figure 4
Defective GC recruitment and retention of sap−/− T cells due to lack of efficient cognate interactions with B cells
To address whether the inefficient GC recruitment and retention of sap−/− T cells resulted from their reduced antigen-specific interactions with B cells, radiation bone-marrow chimeras were constructed so that endogenous B cells were deficient in class II MHC expression and thus unable to engage in antigen-specific interactions with T cells, while T cells could still interact with and be activated by non-B antigen presenting cells including DCs (Supplementary Fig. 8; see a methodological diagram in Fig. 4d). Following co-transfer of both sap+/+ and sap−/− OT-2 T cells together with MD4 B cells into these chimeric animals, they were immunized with a mixture of HEL-BSA and intact OVA proteins. In this setting, MD4 B cells cannot engage transferred OT-2 T cells as cognate partners but can still form GCs (Supplementary Fig. 9a) by utilizing cognate help from endogenous CD4+ T cells. OT-2 cells in these mice can be normally activated by OVA-presenting DCs, but are deprived of cognate interactions with B cells, which are either transferred MD4 cells that do not present OVA or endogenous B cells that are derived from class II MHC-deficient bone marrow progenitors. While both sap+/+ and sap−/− OT-2 T cells upregulated CXCR5 and ICOS expression in this experimental system (Supplementary Fig. 9b), now they both failed to be efficiently recruited into and retained within GCs (Figs. 4e and 4f, Supplementary Fig. 7, Supplementary Movies 7 and 8). When the same chimeric mice were immunized with HEL-OVA, a condition in which OT-2 T cells could engage MD4 B cells as cognate partners, sap+/+ OT-2 T cells were recruited to the GC, while sap−/− OT-2 T cells were not (data not shown). These findings indicate that T cells require cognate interactions with B cells to be recruited into and retained within GCs and suggest that, by failing to engage productively in such cell-cell interactions, SAP-deficient T cells are unable to physically localize to the GC to effectively sustain the GC reaction.
This study demonstrates that SAP expression in T cells is critical for stable antigen-dependent T-B adhesion but dispensable for T-DC interactions, revealing that distinct molecular rules govern the stable interactions of CD4+ T cells with two major antigen presenting cell types in vivo. Stabilization of T-B interactions may rely on a T cell-autonomous mechanism orchestrated by SAP and its associated SLAM family molecules at the cell-cell interface, while stable T-DC association might be actively promoted via DC-mediated mechanisms, such as those involving cytoskeletal and membrane mechanics38,39. In accord with this notion, B cells but not DCs express high levels of SLAM, CD84, Ly9, and Ly108 molecules (Supplementary Fig. 10), which are also highly expressed by T cells activated in vitro and by TFH cells in vivo (ref. 40 and data not shown). The differential SAP regulation of T-DC and T-B interactions correlates with our observations that CD4+ T cell activation in vivo is grossly normal irrespective of SAP expression, while otherwise competent effector T cells fail to efficiently deliver contact-dependent help to cognate B cells in the absence of SAP. At the cellular level, these results provide a likely explanation for why SAP deficiency specifically impairs T-dependent humoral immunity but leaves intact or even exaggerates cell-mediated immune responses that also require CD4+ T cell activity1315,41,42. While additional factors may also contribute to the overall defects of humoral immunity seen in SAP-deficient hosts43, our results strongly suggest the predominant mechanism is the disruption of antigen-specific T-B adhesion. Future studies are necessary to determine the molecular basis for how SAP selectively controls cognate T-B association, to identify SAP-associated SLAM family proteins involved in this process, and to elucidate the cascade of intercellular signal exchange that transpires across a stable T-B synapse.
In conclusion, our findings offer a compelling explanation for the profound impairment of the GC response in SAP-deficient mice and suggest a mechanism contributing to the B cell-centric pathologies associated with the XLP syndrome. They provide novel insights into the operating principles of intercellular communication between T cells and diverse antigen presenting cells and serve as a striking example of the importance of a direct visualization approach in understanding immune system function in vivo.
Cell conjugation assay in vitro
OT-2 T cells (5×105/well) were incubated for 30 min at 37°C in 96-well U-bottom plates with DCs (106/well) or LPS-activated B cells (2×106 per well) that had been pulsed with antigen. Conjugates were enumerated by flow cytometry after the cell mixture was stained at 4°C for CD4, CD11c, and CD19 and repeatedly washed.
Adoptive transfer, cell phenotyping, and intravital imaging
To visualize T-DC interactions (Fig. 1), OVA323-pulsed DCs were injected subcutaneously at 2×106 per mouse 24 hours prior to intravenous transfer of sap+/+ and sap−/− T cells (3×106 each). Imaging was conducted 12 to 24 hours later. To examine activation phenotypes, 106 DCs and 2×105 GFP-expressing T cells per mouse were used. To visualize interactions between B cells and sap+/+ and sap−/− T cells in the same LN (Fig. 2), 3×106 OT-2 T cells of each genotype were co-transferred into mice together with 5×106 wild-type B cells. Immunization was done 12 hours prior to cell transfer, and imaging was conducted 24 to 36 hours thereafter. To visualize T-B interactions under non-competitive conditions and to assay T and B cell expansion (Fig. 3), 6×104 GFP-expressing OT-2 T cells were co-transferred together with 3×105 CFP-expressing B cells 24 hours prior to immunization. Imaging and cytometric analyses were conducted 60–72 and 96 hours later, respectively. To visualize GC recruitment and retention of T cells (Fig. 4), 3×104 CFP-expressing sap+/+ and 3×104 GFP-expressing sap−/− OT-2 T cells were co-transferred with 3×105 non-fluorescent MD4 B cells. Imaging was conducted 6 to 8 days post immunization. Dye-labelled naïve B cells (2–4×107) were given 1 day before imaging to provide follicle/GC landmarks. The imaging set-up was essentially as previously described44. For imaging sessions longer than 2 hours, the animal’s hydration was maintained by lactated Ringer’s solution given via a catheter. The typical X-Y-Z dimension was 0.5–1.1×0.5–1.1×3 μm, and the time resolution was 30–45s. For experiments involving co-transfer of two types of dye-labelled T cells, the cells were always reciprocally labelled to control for potential dye-induced behavioural differences.
Statistical analysis
The Mann-Whitney rank sum test was used to calculate p values for highly skewed distributions. For Gaussian-like distributions, two-tailed t tests were used.
Supplementary data
Supplementary Figures and Movie Legends
This file contains Supplementary Figures 1-10 and Supplementary movie legends.
Supplemental Methods
Supplementary Movie 1
Supplementary Movie 2
Supplementary Movie 3
Supplementary Movie 4
Supplementary Movie 5
Supplementary Movie 6
Supplementary Movie 7
Supplementary Movie 8
Acknowledgments
H.Q. is in debt to Y. Hong for support, encouragement, and inspiration. This work was funded by the intramural research programs of the NIAID and NHGRI, NIH, U.S.A.
Footnotes
Reprints and permission information is available online at www.nature.com/reprints. The authors declare no competing financial interests.
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