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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2009 February 17.
Published in final edited form as:
PMCID: PMC2644261
NIHMSID: NIHMS81230

Distinct Regions of Prefrontal Cortex Mediate Resistance and Vulnerability to Depression

Abstract

The neuroanatomical correlates of depression remain unclear. Functional imaging data have associated depression with abnormal patterns of activity in prefrontal cortex (PFC), including the ventromedial (vmPFC) and dorsolateral (dlPFC) sectors. If vmPFC and dlPFC are critical neural substrates for the pathogenesis of depression, then damage to either area should affect the expression of depressive symptoms. Using patients with brain lesions we show that, relative to nonfrontal lesions, bilateral vmPFC lesions are associated with markedly low levels of depression, whereas bilateral dorsal PFC lesions (involving dorsomedial and dorsolateral areas in both hemispheres) are associated with substantially higher levels of depression. These findings demonstrate that vmPFC and dorsal PFC are critically and causally involved in depression, although with very different roles: vmPFC damage confers resistance to depression, whereas dorsal PFC damage confers vulnerability.

Keywords: depression, emotion, prefrontal cortex, ventromedial, dorsolateral, neuropathology

Introduction

A primary aim in the neuroscientific study of affective disorders is to identify the responsible dysfunctional brain circuits. In particular, the role of prefrontal areas in depression has been a major focus of previous work (Drevets, 1998, 2007; Davidson et al., 2002). Perhaps the most widely accepted division of prefrontal cortex, based on anatomical connectivity and functional specialization, is between the dorsolateral and ventromedial sectors (Rosenkilde, 1979; Kringelbach and Rolls, 2004; Zald, 2007). Neuroimaging studies of resting state activity associate depression with abnormally high levels of ventromedial prefrontal cortex (vmPFC) activity (Drevets et al., 1992; Biver et al., 1994; Nofzinger et al., 2005; Greicius et al., 2007), but abnormally low levels of dorsolateral prefrontal cortex (dlPFC) activity (Baxter et al., 1989; Bench et al., 1993; Biver et al., 1994; Galynker et al., 1998; Mayberg et al., 1999). These resting state data suggest that the pathogenesis of depression may depend on relative hyperactivity in vmPFC and hypoactivity in dlPFC (Mayberg, 1997), although more recent studies using cognitive activation paradigms reveal a more complex profile of PFC activity (e.g., Wagner et al., 2006). Regardless, imaging data alone cannot determine whether the abnormal activity in either vmPFC or dlPFC reflects the underlying cause of the disorder or a secondary effect of the disorder. The question of causality, however, can be addressed through the study of humans with focal brain lesions. If vmPFC hyperactivity and dlPFC hypoactivity are indeed causally involved in the pathogenesis of depression, then damage to either area would presumably affect the development of depression, but with opposite effects. More specifically, vmPFC lesions would confer resistance to depression, whereas dlPFC lesions would confer vulnerability to depression.

To test this prediction, we assessed the severity of depressive symptoms in individuals with focal lesions involving either vmPFC or dorsal PFC. Previous studies investigating the effect of discrete brain lesions on depression have yielded mixed results with respect to lesion location and depression severity. Initial studies in stroke patients reported an association between left anterior lesions and poststroke depression (Robinson et al., 1984; Parikh et al., 1988), but multiple subsequent studies failed to replicate this finding (for review, see Singh et al., 1998; Carson et al., 2000; Yu et al., 2004) and meta-analyses of poststroke depression studies reveal no clear association between lesion location and depression (Carson et al., 2000; Yu et al., 2004). The lack of a consistent relationship between lesion location and depression in these studies could be due factors such as the following: (1) nearly all patients in these studies suffered unilateral damage, so the contralesional hemisphere could potentially provide some compensation of function, (2) in some studies patients were assessed only in the acute recovery phase, so in these cases the reported effects may not endure in the chronic epoch, and (3) none of these studies differentiated between ventromedial and dorsolateral PFC lesions. Here, we report the first study examining the effects of chronic, focal, bilateral lesions of vmPFC and dorsal PFC on depression severity.

Materials and Methods

Patient registries

We studied two separate samples of patients with focal brain lesions. One sample is the Vietnam Head Injury Study (VHIS) registry, which is comprised of American veterans who suffered brain damage mainly from penetrating head injuries in the Vietnam War. The other sample is the Patient Registry of the Cognitive Neuroscience Division at the University of Iowa, which is comprised largely of Iowans who suffered brain damage from ischemic stroke, subarachnoid hemorrhage, or neurosurgical resection. Because the two patient samples differ with respect to gender, lesion onset, and experience of emotional trauma, convergent findings from these two samples would elucidate in compelling manner the relative roles of PFC subregions in depression.

VHIS participants

We drew one set of participants from the W.F. Caveness VHIS registry, which originally included 1221 American soldiers who survived penetrating brain wounds suffered in Vietnam. The VHIS has been organized in three phases. Phase 1 was the initial enrollment, which occurred between 1967 and 1970. For Phase 2, the 1118 veterans still alive were invited to participate in an extensive follow-up clinical study at Walter Reed Army Medical Center between August 1981 and August 1984. Of the 1118 survivors, 520 participated in the Phase 2 study. Comparison subjects (n = 85) were recruited from VA files of non-head-injured soldiers who had served in Vietnam the same years and were within the same age range as soldiers on the Caveness registry. One-hundred and ninety-nine head-injured and 54 non-head-injured subjects from Phase 2 participated in Phase 3, which included a psychiatric evaluation by a neuropsychiatrist (Vanessa Raymont). We conducted Phase 3 between April 2003 and November 2006 at Bethesda National Naval Medical Center. Preinjury characteristics of the participants were available from military and VA records. All subjects gave informed written consent before completing the study.

VHIS lesion analysis

We acquired CT data during the Phase 3 testing period. We acquired Axial CT scans without contrast at Bethesda Naval Hospital on a GE Medical Systems Light Speed Plus CT scanner in helical mode. We reconstructed the images with an in-plane voxel size of 0.4 mm × 0.4 mm, overlapping slice thickness of 2.5 mm and a 1 mm slice interval. We determined lesion location and volume from CT images using the Analysis of Brain Lesion (ABLe) software (Makale et al., 2002; Solomon et al., 2007) contained in MEDx v3.44 (Medical Numerics) with enhancements to support the Automated Anatomical Labeling (AAL) atlas (Tzourio-Mazoyer et al., 2002). We defined lesions by manual tracing in all relevant slices of the CT image. A trained neuropsychiatrist (Vanessa Raymont) performed the tracings, which were then reviewed by an observer (Jordan Grafman) who was blind to the results of the clinical evaluations. The CT image of each brain was spatially normalized to a CT template brain image in MNI space (Collins et al., 1994). Individuals’ normalized scans were used for the computation of group overlaps. We defined two regions of interest (ROIs): the vmPFC and the dlPFC. The vmPFC ROI was defined as those areas of PFC inferior to z = 0 and medial to x = 20 and x = −20. These criteria outlined an area comprising the ventral portion of the medial prefrontal cortex (below the level of the genu of the corpus callosum) and medial portion of the orbital surface (approximately the medial one-third of the orbitofrontal cortex in each hemisphere) as well as the subjacent white matter. The dlPFC ROI was defined as those areas of PFC superior to z = 0 and lateral to x = −10 and x = 10. A patient was included in the vmPFC group if his lesion occupied vmPFC in both hemispheres, but did not occupy any portion of dlPFC in either hemisphere. A patient was included in the dorsal PFC group if his lesion occupied dlPFC in both hemispheres, but did not occupy any portion of vmPFC in either hemisphere. The selection of bilateral lesions precluded the possibility that normal function could be sustained by the intact contralesional hemisphere.

VHIS patient group characteristics

Based on the aforementioned neuroanatomical criteria, we identified seven patients with damage to vmPFC in both hemispheres, but no damage to dlPFC in either hemisphere (“vmPFC lesion group”) (Fig. 1), and we identified five patients with damage to dlPFC in both hemispheres, but no damage to vmPFC in either hemisphere (“dorsal PFC lesion group”) (Fig. 1). Because damage to dlPFC in both hemispheres was always accompanied by damage to the intervening cortex (dorsomedial PFC), we refer to this group as the “dorsal PFC lesion group.” In addition, we studied veterans with lesions not involving PFC (“non-PFC lesion group”; n = 101) and veterans with no brain damage (“non-brain-damaged group”; n = 54). Among the non-PFC lesion group, damage primarily affected the lateral aspect of cerebral cortex. Lesions in this group were distributed throughout temporal, parietal, and occipital cortex, but largely spared deep structures such as basal ganglia, insula, and thalamus. Groups were well matched for age, education, basic cognitive function, and preservice cognitive function (Table 1). Lesion patient groups were well matched for cognitive decline after injury (Table 1). Lesion volume for the vmPFC group (mean 40.1 cm3, SD 19.6) was not significantly different from lesion volume for the dorsal PFC group (mean 62.0 cm3, SD 22.3) (t = −1.8; p = 0.10).

Figure 1
Lesion overlaps of VHIS prefrontal lesion groups. Color indicates the number of overlapping lesions at each voxel. Top row, Transverse slices of a normal healthy brain, for reference. From left to right, z =−22, −12, −2, 8, 18, ...
Table 1
VHIS patient group characteristics

VHIS depression assessment

We assessed the VHIS participants with three independent measures of depression: (1) the Beck Depression Inventory, Second Edition (BDI-II) (Beck et al., 1996) (2) the Structured Clinical Interview for DSM-IV-TR Axis I disorders, Non-Patient edition (SCID-N/P) (First et al., 2002), and (3) the Neurobehavioral Rating Scale (NRS) (Levin et al., 1987). The BDI-II is a 21-item self-report instrument for measuring the severity of specific symptoms of depression. Participants rate each item on a scale of 0–3, with greater numbers indicating greater severity. An overall BDI score (0–63) is derived by summing the severity ratings for each item. In the BDI-II manual, Beck et al. (1996) report the mean BDI-II scores for groups of individuals with different levels of depression severity, as determined by clinical evaluation. “Non-depressed” individuals had mean BDI-II scores of 7.7 (SD 5.9), whereas “mildly depressed,” “moderately depressed,” and “severely depressed” individuals had mean BDI-II scores of 19.1 (SD 5.7), 27.4 (SD 10.0), and 33.0 (SD 12.0), respectively. Thus, for our study, we interpreted any BDI-II score of 8 or below (>2 SD below the mean for “mildly depressed” individuals) as a negligible level of depression (“low” depression severity), and any score of 20 or above as clinically significant depression (“high” depression severity). Scores 9 through 19 were considered “intermediate” depression severity.

The SCID-N/P is a structured interview used for making DSM-IV Axis I diagnoses. A psychiatrist (Vanessa Raymont) used the SCID-N/P to evaluate each participant for major psychiatric disorders.

The NRS is a 27-item instrument for measuring the severity of behavioral sequelae of head injury. One item pertains specifically to depressed mood. The rater assesses the severity of depressive mood from 1 (not present) to 7 (extremely severe) based on the spontaneous behavior of the patient (i.e., the rater did not question the patient specifically about depressed mood). For this study the NRS was completed by research assistants trained in neuropsychological assessment. The research assistants completed the NRS after interacting with the participant for ~25 h over a 5 d period.

Iowa participants

We drew a second set of participants from the Patient Registry of the Division of Cognitive Neuroscience at the University of Iowa. All participants in the group analysis had focal, stable lesions that could be clearly identified on MR or CT scans, and they were free of dementia, substance abuse, and premorbid psychiatric disorder. All participants were capable of comprehending the test instructions and cooperating with the experiment. Neuropsychological and neuroanatomical studies were all conducted in the chronic phase of recovery (more than = months after lesion onset). All participants gave informed consent before completing the study.

Iowa lesion analysis

We determined lesion location from CT or MR images. We initially screened the Iowa Patient Registry for individuals with bilateral vmPFC lesions by selecting those patients with lesions occupying F11 and/or F12 in both hemispheres, according to the Damasio and Damasio (1989) templates. From these patients we selected patients with bilateral damage to same areas described for the VHIS patients: the ventral portion of the medial prefrontal cortex (below the level of the genu of the corpus callosum) and medial portion of the orbital surface (approximately the medial one-third of the orbitofrontal cortex in each hemisphere) as well as the subjacent white matter. Patients were excluded if their lesions extended to the lateral surface in region F07 (the dlPFC) or significantly outside of PFC. Lesion etiologies for the vmPFC group were tumor resection (n = 8) and anterior communicating artery aneurysm rupture/clipping (n = 5). Two of the vmPFC patients had lesions that extended out of PFC; one patient’s lesion included right superior anterior temporal lobe, and another patient’s lesion included right caudate. The non-frontal comparison group was comprised of those individuals who did not have any damage to frontal areas (F01–F14) and who had completed either the Beck Depression Inventory, First Edition Amended (BDI-IA) (Beck and Steer, 1993) or the BDI-II. Lesion etiologies for the nonfrontal group were primarily ischemic stroke, hemorrhage, or surgical resection.

Iowa patient characteristics

In the Iowa neurological registry, we identified 13 patients with damage to vmPFC in both hemispheres, but no significant damage to dlPFC in either hemisphere (“Iowa vmPFC lesion group”). There were no patients in the Iowa registry with focal lesions involving dlPFC in both hemispheres. For comparison purposes, we identified those patients with lesions not involving frontal cortex (“Iowa nonfrontal lesion group”; n = 238). Lesions in the nonfrontal group included lateral areas of temporal, parietal, and occipital cortex (e.g., MCA strokes), temporal pole (e.g., temporal lobectomies), medial occipital cortex (e.g., PCA strokes), as well as basal ganglia and thalamus (e.g., hemorrhage). The two groups did not significantly differ with respect to sex, age of lesion onset, education, or basic cognitive function after brain injury (Table 2).

Table 2
Iowa patient group characteristics

Iowa depression assessment

We assessed depression with either the BDI-IA or the BDI-II. The two versions are similar but not identical: between the two versions (both of which have 21 items), there are 17 conserved items but 4 different items. In the vmPFC group, 6 patients completed the BDI-IA and 7 completed the BDI-II. In the nonfrontal comparison group, 118 patients completed the BDI-IA and 120 completed the BDI-II. For overall BDI scores, we converted BDI-IA scores to BDI-II scores according to the conversion chart published in BDI-II manual (Beck et al., 1996). We used the same criteria as in the VHIS study to classify overall scores as indicating “low” (0–8), “intermediate” (9–19), or “high” (20–63) levels of depression.

Results

VHIS patient depression data

To test our hypothesis that vmPFC damage would reduce depression severity whereas dorsal PFC damage would increase depression severity, we used Fisher’s exact test to determine if the proportions of individuals with “low” or “high” levels of depression severity differed among VHIS patient groups (Fig. 2). The proportion of individuals with “low” depression severity in the vmPFC group (7 of 7) was significantly greater than in the dorsal PFC lesion group (1 of 5; p = 0.005), non-PFC lesion group (65 of 101; p = 0.05), and non-brain-damaged group (24 of 54; p = 0.006). In contrast, the proportion of individuals with “high” depression severity in the dorsal PFC group (3 of 5) was significantly greater than in the vmPFC lesion group (0 of 7; p = 0.045) and non-PFC lesion group (10 of 101; p = 0.012), and marginally greater than the non-brain-damaged group (13 of 54; p = 0.10). These data indicate that bilateral vmPFC damage was associated with relatively low depression severity, whereas bilateral dorsal PFC damage was associated with relatively high depression severity.

Figure 2
VHIS BDI-II scores. y-Values indicate the proportion of individuals who were classified as “low” (white bars), “intermediate” (light gray bars), or “high” (dark gray bars) depression severity for each group. ...

As a complementary analysis, we performed a parametric analysis of the BDI-II scores, and found that mean depression scores differed significantly among groups (F = 3.3; p = 0.021). The specific pattern of results was identical to the nonparametric analysis: the mean BDI-II score of the vmPFC lesion group (4.4) was significantly lower than the mean BDI-II score of both the non-PFC lesion group (8.9; t = −3.3; p = 0.004) and non-brain-damaged group (11.6; t = −4.3; p < 0.001), whereas the mean BDI-II score of the dorsal PFC lesion group (19.0) was significantly greater than the mean BDI-II score of the non-PFC lesion group (t = 2.3; p = 0.021), and marginally greater than the non-brain-damaged group (t = 1.6; p = 0.12). The parametric analysis thus confirms the differential effects of vmPFC and dorsal PFC lesions on depression.

One possible reason for the between-group differences in BDI-II scores could be a difference in the prevalence of posttraumatic stress disorder (PTSD) at the time of the BDI-II assessment. PTSD is commonly associated with symptoms of depression (Regier et al., 1998; Devane et al., 2005), and vmPFC damage is associated with a reduction in PTSD among combat veterans (Koenigs et al., 2008). Thus, the absence of higher BDI-II scores in the vmPFC group could be attributable to the absence of PTSD in this group. Indeed, none of the seven vmPFC patients were diagnosed with concurrent PTSD at the time of BDI administration, whereas 13/101 in the non-PFC lesion group, 1 of 5 in the dorsal PFC lesion group, and 15 of 54 in the non-brain-damaged group were diagnosed with concurrent PTSD at the time of the BDI administration. After removal of these PTSD-positive individuals, Fisher’s exact test showed that the proportion of “low” depression severity individuals in the vmPFC group remained significantly greater than the dorsal PFC lesion group (p = 0.02) and non-brain-damaged group (p = 0.04), and marginally greater than the non-PFC lesion group (p = 0.09). These data indicate that the absence of PTSD did not fully account for the abnormally low BDI-II scores in the vmPFC group.

Because depression is a disorder involving multiple characteristic symptoms, which may be experienced in varying degrees, the observed overall reduction in BDI-II scores among the vmPFC patients and overall elevation in BDI-II scores among the dorsal PFC patients raise an important follow-up question: does vmPFC or dorsal PFC damage affect all depressive symptoms to a similar extent, or are the differences in overall BDI-II score driven by a subset of symptoms? To answer this question, we compared the groups’ mean severity ratings for each of the 21 items of the BDI-II (Fig. 3). Multiple studies report a two-factor structure for the BDI-II: one factor of “cognitive/affective” items (e.g., sadness, worthlessness, self-dislike) and another factor of “somatic” items (e.g., changes in appetite, tiredness, concentration difficulty) (Beck et al., 1996; Steer et al.,1999; Whisman et al., 2000; Storch et al., 2004) (see also Buckley et al., 2001; Vanheule et al., 2008). The 14 items receiving the lowest self-reported severity among vmPFC patients were exactly the same 14 items that loaded onto the original “cognitive/affective” factor reported in BDI-II manual (Beck et al., 1996). There was a significant difference among groups for mean ratings of the “cognitive/affective” items (F = 2.8; p = 0.04). For these items, the vmPFC lesion group had a significantly lower mean score (0.07) than either the non-PFC lesion group (0.35; t = −4.5; p < 0.001) or the non-brain-damaged group (0.48; t = −5.1; p < 0.001). The vmPFC lesion group did not have significantly lower scores for the remaining (primarily “somatic”) items (F = 1.7; p = 0.17). This analysis indicates that bilateral vmPFC damage selectively diminishes the cognitive/affective symptoms of depression.

Figure 3
Mean severity scores for individual BDI-II items. The 14 items to the left of both dashed lines are “cognitive/affective” symptoms, whereas the 5 items to the right of both dashed lines are the “somatic” symptoms. The 2 ...

As a second independent measure of depression in the VHIS patients, a psychiatrist assessed whether or not each participant met the DSM-IV criteria for “lifetime” and/or “current” major depressive disorder (MDD) using the SCID-N/P. The SCID-N/P includes “lifetime” and “current” diagnoses for other mood disorders including bipolar affective disorder (I and II), dysthymic disorder, and depressive disorder not-otherwise-specified (DD-NOS). The “lifetime” diagnosis pertained to any point in the participant’s life, including preinjury service in Vietnam. As such, the “lifetime” diagnosis is not necessarily informative with respect to the effect of brain injury on depression. In contrast, a diagnosis of “current” MDD unambiguously indicates the presence of symptoms after the injury. The SCID data for mood disorders are presented in Table 3. The proportion of individuals meeting DSM-IV criteria for “lifetime” MDD was not significantly different between groups (Fisher’s exact test; all p values > 0.16), nor were the proportions of individuals meeting DSM-IV criteria for other “current” mood disorders (Fisher’s exact test; all p values > 0.21). However, the proportion of individuals meeting DSM-IV criteria for “current” MDD was significantly greater for the dorsal PFC lesion group (2 of 5) than for the non-PFC lesion group (1 of 101; p = 0.005) or non-brain-damaged group (0 of 54; p = 0.006). Thus, bilateral dorsal PFC lesions were associated with a relatively high prevalence of subsequent major depression.

Table 3
SCID-N/P assessment of Axis I depressive disorders

As a third measure of depression, each VHIS patient was assessed with the NRS, which includes one item pertaining specifically to depressed mood. There were no significant differences in the rating of depression severity on the NRS among the five groups (F = 0.43; p = 0.79). Although the between-group differences were not significant, the pattern of depression severity among groups was consistent with the BDI-II and SCID-N/P data: the mean rating of depression severity for the vmPFC lesion group (1.17) was lower than the non-PFC lesion group (1.41), non-brain-damaged group (1.37), and dorsal/ventral PFC lesion group (1.33), whereas the mean rating of depression severity for the dorsal PFC lesion group was higher (1.80).

Relationship of depression and cognitive or motor impairment

One possible explanation of the relatively high depression severity among veterans with lesions involving dorsal PFC is that their symptoms of depression could be a consequence of significant impairment in cognitive and/or motor function. To address this possibility, we used a multiple regression analysis to determine whether BDI-II score among all brain-injured VHIS patients was significantly predicted by neuropsychological measures of cognitive function or neurological measures of motor function. We included the following measures in the analysis: MMSE, a gross measure of intellectual function; AFQT change, a measure of overall cognitive decline; Boston Naming Test, a measure of object naming and aphasia; Token Test, a measure of language comprehension; Verbal Fluency, a measure of executive function; and the presence/absence of ataxia, paresis, and voluntary movement abnormality. The model was not significant (F (8,147) = 1.5, p = 0.18), and explained only 2.3% of the variance in BDI-II score (adjusted R2 = 0.023), indicating that none of the measures of cognitive or motor function were strong predictors of BDI-II score in the VHIS patient sample. These results suggest that the elevated depression among veterans with dorsal PFC damage is not simply an affective response to cognitive or motor impairment.

Iowa patient depression data

Given the considerable effect of PFC lesion location on depressive symptomotology among veterans in the VHIS, we sought to determine whether the same pattern would be observed in a different population of patients with PFC lesions. The proportion of individuals with “low” depression severity in the Iowa vmPFC group (12 of 13) was significantly greater than in the Iowa nonfrontal comparison group (109 of 238; p = 0.001) (Fig. 4). Again we confirmed this finding with a parametric test: the mean BDI score of the Iowa vmPFC lesion group (4.7) was significantly lower than the mean BDI score of the Iowa nonfrontal lesion comparison group (10.3; t = −5.3; p < 0.001). These results mirror the VHIS data; focal bilateral vmPFC damage was associated with relatively low depression severity.

Figure 4
Iowa BDI scores. y-Values indicate the proportion of individuals who were classified as “low” (white bars), “intermediate” (light gray bars), or “high” (dark gray bars) depression severity for each group. ...

Again, we determined whether bilateral vmPFC damage was associated with a reduction in particular symptoms of depression (supplemental Table 1, available at www.jneurosci.org as supplemental material). The 13 bilateral vmPFC patients from the Iowa registry uniformly endorsed the complete absence of three symptoms: sadness, loss of interest, and feelings of punishment, all of which load onto the “cognitive/affective” factor. The Iowa vmPFC patient group’s highest ratings of symptom severity were for change in appetite and tiredness, which load onto the “somatic” factor, and pessimism, which loads onto neither factor. Thus, like the VHIS patients with focal bilateral vmPFC lesions, the Iowa vmPFC patients exhibit less severe “cognitive/affective” symptoms than “somatic” symptoms of depression.

In addition, we identified one patient in the Iowa registry who represents an intriguing case of an apparent alleviation of severe depression after a bilateral vmPFC lesion. Although no records of formal psychiatric assessments were available, per secondary report the patient was being treated for depression when she attempted suicide 11 years ago by means of a gunshot to the head. The gunshot destroyed most of ventral PFC, including vmPFC bilaterally, but left intact most of dorsal PFC (supplemental Fig. 1, available at www.jneurosci.org as supplemental material). The patient’s neuropsychologist, neurosurgeon, and long-term boyfriend all remarked that her depression was markedly diminished after the brain injury (boyfriend, speaking 16 months after the injury: “no sign of depression whatsoever since the accident”; neuropsychologist: “she never shows distress, worry, or anger”). Her postinjury self-report data, collected at the University of Iowa 16 months after the injury, corroborate this impression. On the BDI-IA, the patient had a total score of 11. The specific items she endorsed were severe loss of libido; moderate levels of fatigability and indecisiveness, and mild levels of sense of failure, self-accusations, irritability, and weight loss. She endorsed the complete absence of 14 items, including sadness, self-dislike, guilt, and suicidal ideation. Despite the lack of data from formal pre-injury and postinjury psychiatric assessments, which precludes any definitive conclusions regarding the effect of the lesion on her mood state, the available data for this case are consistent with vmPFC playing a causal role in the experience of “cognitive/affective” symptoms of depression.

Discussion

In sum, we found opposite effects of vmPFC and dorsal PFC damage on depression. In the VHIS patient sample, veterans with bilateral vmPFC damage reported significantly lower depression severity than did veterans with damage involving other areas of the brain or veterans with no brain damage, particularly for the cognitive/affective symptoms of depression. This finding was replicated in the Iowa sample: neurological patients with bilateral vmPFC damage reported significantly lower depression severity than did patients with damage involving posterior areas of the brain. In addition, one patient with a premorbid history of depression experienced a dramatic alleviation of depressive symptoms, particularly the cognitive/affective symptoms, after bilateral vmPFC damage. Conversely, VHIS patients with bilateral dorsal PFC damage reported significantly greater depression severity than veterans with damage involving other areas of the brain and were more frequently diagnosed with current major depression than were veterans with damage involving other areas of the brain or veterans with no brain damage. VHIS patients with unilateral dlPFC damage also reported relatively high levels of depression severity (supplemental results; supplemental Fig. 2, available at www.jneurosci.org as supplemental material). These data support the prediction that vmPFC lesions would confer resistance to depression whereas dlPFC lesions would confer susceptibility to depression, and suggest that vmPFC and dlPFC are causally involved in the experience of depression.

The possible mechanisms for the differential effects of vmPFC and dorsal PFC lesions on depression warrant discussion. First we consider the finding of diminished depression among vmPFC patients. Previous lesion studies associate vmPFC damage with a marked lack of self-insight (Barrash et al., 2000; Beer et al., 2006), as well as a reduction in certain types of negative affect, particularly shame, guilt, embarrassment, and regret (Camille et al., 2004; Beer et al., 2006; Koenigs et al., 2007). One common feature of these emotions is that they all involve an element of self-awareness or self-reflection. We speculate that the observed lack of depression among vmPFC patients could be attributable to an impairment of self-insight or self-reflection. Data from specific BDI items support this interpretation: vmPFC patients reported conspicuously low levels of “cognitive/affective” symptoms (such as guilt, self-dislike, and sadness) but normal levels of “somatic” symptoms (such as fatigue and changes in sleeping or appetite). Because the “cognitive/affective” items ostensibly involve a greater degree of self-reflection and rumination than the “somatic” items, one can conceive of the vmPFC patients’ lack of depression as a secondary effect of a primary lack of self-awareness and self-reflection. This interpretation holds that vmPFC is critical for self-insight and thus also critical for the self-reflective negative affect that is fundamental to depression; a loss of this capacity of self-insight/self-reflection after vmPFC damage may reduce many symptoms of depression.

Impairment in self-insight/self-reflection could also theoretically account for our previous finding of diminished PTSD among veterans with vmPFC lesions (Koenigs et al., 2008), as PTSD is characterized by the distressful experience of negative affect (e.g., anxiety, guilt) associated with the rumination of past events. The finding of both diminished depression and diminished PTSD among patients with vmPFC lesions supports the assertion that mood and anxiety disorders share a common higher order dimension of general distress or “negative affectivity” (Clark and Watson, 1991; Watson, 2005), and moreover, that vmPFC may be a critical neural substrate for this common dimension, perhaps by virtue of its role in self-insight. An interesting follow-up study would be to determine whether vmPFC patients exhibit certain objective (behavioral or physiological) correlates of depression or anxiety while reporting no subjective experience of depressed or anxious mood.

With respect to the elevated depression severity among VHIS patients with dorsal PFC damage, we believe our results are consistent with recent functional imaging data demonstrating the recruitment of dlPFC during the regulation of negative emotion through reappraisal/suppression strategies (Ochsner et al., 2002, 2004; Lévesque et al., 2003; Phan et al., 2005; Eippert et al., 2007). If the reappraisal/suppression of negative affect is a protective mechanism against depression, and if dlPFC is necessary for this function, then one would expect elevated depression among individuals with dlPFC damage. We believe that a defect in the regulation of negative affect attributable to dlPFC damage is indeed a plausible mechanism for the observed elevation in depression in the dorsal PFC lesion group.

However, it is important to point out limitations on the conclusions that can be made with respect to the role of dlPFC in depression, based on the results of this study. For one, the patients did not have “pure” bilateral dlPFC lesions. Since the intervening cortex (the dorsomedial PFC) was also damaged in each of the dorsal PFC patients, the contribution of dorsomedial PFC damage to the observed results cannot be discounted. Second, although both parametric and nonparametric analyses of BDI scores indicate greater depression in the dorsal PFC lesion patients than in the non-PFC lesion patients, the difference in BDI scores between the dorsal PFC lesion patients and the non-brain-damaged comparison subjects did not reach significance. However, the proportion of dorsal PFC lesion patients diagnosed with current MDD was significantly greater than that of either comparison group, including the non-brain-damaged subjects. Finally, all patients in the dorsal PFC lesion group were adult males, so the findings may not generalize to all cases of depression. Given these limitations, we regard our conclusions about the causal role of dlPFC in depression as preliminary, pending replication in additional patient samples.

One unique feature of this study is the sample sizes of the target groups: 20 cases of focal bilateral vmPFC damage (seven in the VHIS registry, 13 in the Iowa registry) and five cases of bilateral dlPFC damage. The 20 total patients with focal, bilateral vmPFC damage is, to our knowledge, the largest such group ever reported in a single study. And to our knowledge, ours is also the first study to assemble a group of patients with focal lesions involving dlPFC in both hemispheres. Moreover, the relatively large sample sizes of key comparison groups (101 VHIS patients with non-PFC lesions, 54 veterans with no brain damage, and 238 Iowa patients with nonfrontal damage) provide a stable point of reference and allow for meaningful statistical analyses. Nonetheless, it will be important to corroborate our observed pattern of results with additional cases of prefrontal damage.

A second unique feature of this study is the use of two vmPFC patient samples with distinct characteristics. Whereas all VHIS patients are men of similar age who experienced emotionally traumatic events and lesion onset in early adulthood, the Iowa patients are both men and women with various ages of lesion onset who did not systematically experience emotional trauma before their brain injuries. The fact that nearly identical results were obtained in both samples of vmPFC patients supports the generalizability of the findings, and allows us to rule out a host of attribute variables as likely explanations for the observed results.

It is noteworthy that the case study presented in this study (the woman who evinced a dramatic alleviation of depressive symptoms after a failed suicide attempt that inflicted bilateral vmPFC damage) bears remarkable resemblance to a previously reported case (Ellenbogen et al., 2005). In this case, a 33-year-old man attempted suicide with a crossbow, inflicting severe damage within vmPFC. Afterward he was “indifferent to his situation and inappropriately cheerful.” Together, these two extraordinary cases underscore the critical and causal role that vmPFC plays in mediating negative affect.

Furthermore, the findings of this study accord with previous studies of neuroanatomically specific treatments for mood and anxiety disorders, such as psychosurgery and exogenous brain stimulation. Subcaudate tractotomy, a surgical procedure that interrupts white matter tracts connecting the vmPFC to subcortical structures, has shown efficacy in reducing symptoms of depression and anxiety (Cosgrove, 2000; Shields et al., 2008). And as would be predicted by the results of our study, vegetative symptoms of depression (e.g., changes in appetite, sleep, and energy) were less likely to improve with subcaudate tractotomy than depressed mood or anxiety (Hodgkiss et al., 1995). Similarly, a study of deep-brain stimulation reports antidepressant effects of inhibiting subgenual PFC white matter (Mayberg et al., 2005), which is adjacent to and interconnected with the vmPFC area described in our study. In addition, studies of transcranial magnetic stimulation have reported antidepressant effects of stimulating dlPFC (Berman et al., 2000; Herwig et al., 2003; Avery et al., 2006). Our results support the notion that treatments aimed at selectively inhibiting vmPFC and/or stimulating dlPFC could have efficacy in alleviating depression.

Supplementary Material

Supp Info

Acknowledgments

This work was supported in part by the National Institute of Neurological Disorders and Stroke (NINDS) intramural research program, DAMD17-01-1-0675 (J.G.), National Institute on Drug Abuse Grant R01 DA022549 (D.T.), and NINDS Grant P01 NS19632 (D.T.).

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