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J Clin Microbiol. 1994 October; 32(10): 2590–2592.
PMCID: PMC264111

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

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Selected References

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