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TRPM2 is a tetrameric Ca2+-permeable channel involved in immunocyte respiratory burst and in postischaemic neuronal death. In whole cells, TRPM2 activity requires intracellular ADP ribose (ADPR) and intra- or extracellular Ca2+, but the mechanism and the binding sites for Ca2+ activation remain unknown. Here we study TRPM2 gating in inside-out patches while directly controlling intracellular ligand concentrations. Concentration jump experiments at various voltages and Ca2+ dependence of steady-state single-channel gating kinetics provide unprecedented insight into the molecular mechanism of Ca2+ activation. In patches excised from Xenopus laevis oocytes expressing human TRPM2, coapplication of intracellular ADPR and Ca2+ activated ~50-pS nonselective cation channels; K1/2 for ADPR was ~1 µM at saturating Ca2+. Intracellular Ca2+ dependence of TRPM2 steady-state opening and closing rates (at saturating [ADPR] and low extracellular Ca2+) reveals that Ca2+ activation is a consequence of tighter binding of Ca2+ in the open rather than in the closed channel conformation. Four Ca2+ ions activate TRPM2 with a Monod-Wymann-Changeux mechanism: each binding event increases the open-closed equilibrium constant ~33-fold, producing altogether 106-fold activation. Experiments in the presence of 1 mM of free Ca2+ on the extracellular side clearly show that closed channels do not sense extracellular Ca2+, but once channels have opened Ca2+ entering passively through the pore slows channel closure by keeping the “activating sites” saturated, despite rapid continuous Ca2+-free wash of the intracellular channel surface. This effect of extracellular Ca2+ on gating is gradually lost at progressively depolarized membrane potentials, where the driving force for Ca2+ influx is diminished. Thus, the activating sites lie intracellularly from the gate, but in a shielded crevice near the pore entrance. Our results suggest that in intact cells that contain micromolar ADPR a single brief puff of Ca2+ likely triggers prolonged, self-sustained TRPM2 activity.
TRPM2 belongs to the melastatin-related subfamily of transient receptor potential proteins (Nagamine et al., 1998) and forms a cation-selective ion channel, which is permeable to Na+, K+, and Ca2+ (Perraud et al., 2001; Sano et al., 2001). It is abundantly expressed in the brain and in immune cells of the monocytic lineage and is activated under conditions of oxidative stress. TRPM2 activity plays important roles in the respiratory burst of neutrophil granulocytes and macrophages and in delayed neuronal cell death after ischemic stroke (for review see Nilius et al., 2007).
The TRPM2 protein is 1,503 amino acids long (Nagamine et al., 1998) and forms homotetrameric channels (Maruyama et al., 2007). In addition to the canonical TRPM family sequences, TRPM2 contains a 270–amino acid C-terminal NUDT9-H domain homologous to the ubiquitous mitochondrial NUDT9 enzyme that hydrolyzes ADP ribose (ADPR) into AMP and ribose-5-phosphate (Perraud et al., 2001). TRPM2 channel currents are activated by intracellular ADPR and the isolated NUDT9-H domain is an active ADPR hydrolase (Perraud et al., 2001, 2003). Many other adenine nucleotides have been implicated in channel regulation (Kolisek et al., 2005; Beck et al., 2006; Grubisha et al., 2006); and oxidative stress induced by hydrogen peroxide (H2O2) also leads to TRPM2 activation in intact, TRPM2-expressing cells, although it is still a matter of debate whether H2O2 acts directly on TRPM2 or by perturbing adenine nucleotide metabolism (Wehage et al., 2002; Perraud et al., 2005; Buelow et al., 2008).
Early work has shown that activation of TRPM2 by ADPR is strongly potentiated by Ca2+ (Perraud et al., 2001). Biochemical studies have shown that calmodulin (CAM) binds to the TRPM2 protein; thus, CAM might be involved in the Ca2+ regulation of channel activity (Tong et al., 2006). Both intra- and extracellular Ca2+ enhance TRPM2 currents in whole-cell patch-clamp experiments, but intracellular Ca2+ plays a more prominent role (McHugh et al., 2003). A recent extensive study on Ca2+ dependence of TRPM2 whole-cell currents has led to the postulation of an extracellular Ca2+-binding site on the channel that is involved in its activation when intracellular Ca2+ concentration ([Ca2+]i) is low (Starkus et al., 2007). The mechanism by which Ca2+ regulates TRPM2 activity is not understood.
A caveat to the interpretation of most TRPM2-related studies to date is the limited suitability of the whole-cell patch-clamp technique (Hamill et al., 1981) for studying the biophysics of gating of a channel primarily affected by ligands from the intracellular side. In such studies each experiment is limited to the use of a single composition of intracellular (pipette) solution that gradually dialyzes the cell interior. First, because the rate of current development is limited by the slow time course of this diffusional equilibration (which occurs over tens of seconds), such experiments provide no information on the kinetics of single-channel gating, thus precluding dissection of molecular mechanisms. Second, to construct a dose–response curve, each ligand concentration is tested in a different cell, while channel expression varies from cell to cell. Third, the assumption that the cytosolic face of TRPM2 channels experiences a solution identical to the pipette solution is not necessarily true, given that the majority of the cellular machinery is still in place, including enzymes that produce or metabolize ADPR, compartments that can rapidly take up or release Ca2+, as well as plasma membrane pumps, and channels that may alter submembrane [Ca2+] despite strong exogenous buffering. Thus, because of the complexity of the entire cell it is hard to distinguish direct effects on TRPM2 itself from indirect effects that alter some intracellular factor involved in the regulation of the channel.
To study TRPM2 gating more directly, and at a single-molecule resolution, we have established a system for expressing TRPM2 in Xenopus laevis oocytes and for selective recording of TRPM2 currents in excised inside-out patches in which the concentrations of ligands bathing the cytosolic surface of the channels can be directly and rapidly controlled. With combined analysis of steady-state, single-channel recordings and macroscopic concentration jump experiments at various voltages we have elucidated the mechanism of Ca2+ activation and the spatial location of the binding sites for activating Ca2+ ions on the TRPM2 channel. Our results firmly establish that these “activating sites” are found intracellularly of the gate, but not on the protein surface. Rather, they are buried in a shielded vestibule in immediate vicinity of the mouth of the pore. The affinity for Ca2+ is higher in the open channel state, and binding of four Ca2+ ions results in an overall ~106-fold activation that is well described by the Monod-Wymann-Changeux (MWC) model. Our findings bear important implications for our understanding of how TRPM2 channels respond to metabolic signals in intact cells.
The full-length human TRPM2 cDNA clone, inserted into pCMV6-XL4, was purchased from OriGene Technologies, Inc. The 5.6-kB insert was excised using EcoRI and XbaI and subcloned into the corresponding sites of pGEMHE (Liman et al., 1992). The resulting hTRPM2/pGEMHE construct was confirmed by automated sequencing. For in vitro transcription, plasmid DNA was linearized by Nhe1; complementary RNA (cRNA) was prepared using a T7 mMessage mMachine kit (Applied Biosystems) and quantified on denaturing gels.
Xenopus oocytes were isolated and injected with cRNA as previously described (Chan et al., 2000). Oocytes were defolliculated by collagenase treatment in a Ca2+-free Ringer’s solution and stored at 18°C in Ringer’s supplemented with 1.8 mM CaCl2 and 50 µg/ml gentamycin. To obtain various levels of channel expression, 0.1–10 ng of hTRPM2 cRNA was injected in a constant 50-nl volume and recordings were done 2–3 d after injection.
To determine free [Ca2+] in the micromolar range, we used Ca-Green 5N (Invitrogen) fluorescence (excitation, 505 nm; emission, 535 nm). Apparent binding parameters of this dye at 25°C and pH 7.1 were obtained by stepwise titration with CaCl2 (Fig. S1, available at http://www.jgp.org/cgi/content/full/jgp.200810109/DC1). Using these parameters we then measured a free [Ca2+] of 4.4 µM in our 140-mM sodium gluconate–based bath solution in the absence of high-affinity chelators and without added Ca2+. Careful two-way titrations revealed a Kd for calcium gluconate of ~20 mM (Fig. S2) at 25°C and pH 7.1. Based on this Kd, a fraction ~1/8 of total added Ca2+ is expected to remain free in 140 mM sodium gluconate. Further fluorescent measurements largely confirmed this prediction and verified that free [Ca2+] was not affected by the addition of 32 µM ADPR (Table S1).
Solutions with free [Ca2+] of 8 nM, 30 nM, 100 nM, 300 nM, and 1 µM were prepared based on calculations using Winmaxc and the resulting free [Ca2+] was then verified using FURA-2 (Fig. S3 and Table S2).
Patch pipettes were pulled from borosilicate glass and had resistances of 3–4 MΩ in our pipette solution. The tip of the pipette was filled up to ~1-cm height with a solution containing 140 mM sodium gluconate, 2 mM magnesium gluconate2, and 10 mM HEPES, pH 7.4, with NaOH; for experiments in 1 mM of extracellular Ca2+ we added 8 mM calcium gluconate2. The back of the pipette, containing the electrode, was filled with a solution containing 140 mM NaCl, 2 mM MgCl2, and 10 mM HEPES, pH 7.4, with NaOH. The bath solution (140 mM sodium gluconate, 2 mM magnesium gluconate2, and 10 mM HEPES, pH 7.1, with NaOH) was supplemented with 0 µM, 32 µM, 100 µM, 320 µM, 1 mM, or 3.2 mM calcium gluconate2 to obtain 4.4, 7.6, 14.8, 43.6, 125, and 398 µM free [Ca2+], respectively. To obtain 8 nM, 30 nM, 100 nM, 300 nM, and 1 µM free [Ca2+], we added 1 mM EGTA and 0, 70, 240, 500, and 760 µM Ca(gluconate)2, respectively, and readjusted the pH to 7.1. Na2-ADPR (Sigma-Aldrich) was added from a 32-mM aqueous stock solution. Patches were excised and transferred into a custom-made micromanifold accommodating eight flow lines, connected to the bath electrode through a 140-mM KCl-agar bridge, where the cytoplasmic surface was continuously superfused at 25°C with standard bath solution containing various test substances. This flow chamber had a dead volume of ~8 µl and the flow rate of the bath solution was ~0.5 ml/min. Switching between solutions was implemented by computer-driven electric valves (HEKA) and occurred with minimal mixing; the kinetics of solution exchange was characterized by a delay of ~1 s (because of the dead volume), followed by an abrupt transition period. To measure solution exchange rate, endogenous Ca2+-activated Cl− channel currents were activated by application of 1 mM of intracellular Ca2+ in the presence of a NaCl-based intracellular solution, and the rate of decay of this Cl− current upon rapid removal of intracellular Cl− (Fig. 1 A) was fitted by a single exponential, yielding an estimate of 58 ± 10 s−1 (n = 16) for the rate of solution exchange. Membrane currents were recorded at 25°C, filtered at 2 kHz, digitized at 10 kHz, and saved to disk using an Axopatch-200B amplifier, a Digidata 1322A board, and pCLAMP 9 software (MDS Analytical Technologies).
Unitary current amplitudes (i) were determined by fitting multiple Gaussian functions to all-points histograms. Plots of i versus membrane voltage were fitted by linear regression to obtain slope conductances.
Open probability (Po) can be estimated from stationary noise analysis of macroscopic currents (Sakmann and Neher, 1995). Assuming identical and independent channels and using binomial theory, the mean m(I) and variance σ2(I) of the macroscopic current (I) can be expressed from Po as m(I) = NiPo and σ2(I) = Ni2Po(1 − Po), respectively (N is the number of active channels and i is the unitary current size). From these equations it follows that σ2(I)/i = (1 − Po)m(I). Thus, for a stationary segment of record the ratio between σ2(I)/i and m(I) yields an estimate of 1 − Po (i.e., of Po). To sample the full variance of the macroscopic current caused by single-channel gating fluctuations, a stationary segment must be long compared with the mean time (the cycle time) required for a single channel to visit all possible single-channel states; segments that are too short underestimate the variance.
To estimate the Po of TRPM2 channels during the time course of rundown (Fig. 1 B and Fig. 2 A), we adapted the above approach to make it applicable to situations where quasi-stationary current segments of sufficient length cannot be obtained. Because in longer segments the current declines by a sizeable fraction, the variance of such a segment is the sum of the variance caused by single-channel gating fluctuations and that of the monotonous current drift. To isolate the variance of the gating fluctuations, we first fitted the decaying current I (Fig. 2 A, black trace) with a single exponential (Fig. 2 A, red line), and then subtracted this fit line from the current trace to obtain the time course of gating noise (Î) devoid of macroscopic drifts (Fig. 2 A, blue trace). We then split the experimental time course into segments of length τ ln(5/3), where τ is the time constant of the fitted exponential. Within such a segment the current falls to ~60% of its initial value, i.e., at its two ends it differs by approximately ±20% from its mean. Finally, for each obtained segment of time (Fig. 2 A, colored bars) we plotted −σ2(Î)/i as a function of −m(I) (Fig. 2 B, colored circles).
We tested the new method extensively on simulated records (Fig. S4, available at http://www.jgp.org/cgi/content/full/jgp.200810109/DC1) and found that it was suitable to (a) discriminate between high, intermediate, and low Po values and (b) discern a constant number of channels gating with a gradually declining Po from a progressively declining number of channels gating at constant Po. In particular, for a declining N with constant Po the variance/mean plots produce straight lines (Fig. S4, A–C), whereas in the case of a declining Po these plots are characteristically curved, producing increasing slope values as the current approaches zero (Fig. S4 D).
Currents from segments of record with no more than 10 superimposed channel openings were baseline subtracted, Gaussian filtered at 200 Hz, and idealized by half-amplitude threshold crossing using a 1-ms fixed dead time (Csanády, 2000). Po was calculated from the resulting events lists using the cycle-time method: Po = (Σklktk)/(NT), where lk and tk are the conductance level and duration of the kth event, N is the total number of active channels, and T = Σktk. Although we only studied mean opening (kCO) and closing rates (kOC), defined as the inverses of the mean closed and open times, respectively, we extracted these using a method that includes a correction for the dead time (unlike the cycle-time method). At all Ca2+ concentrations, single-channel gating showed clear bursting behavior; brief closures of ~2-ms duration interrupted bursts of openings flanked by longer, interburst closures. Thus, we chose the C1–C2–O3 scheme to simultaneously fit the dwell-time histograms of all conductance levels by maximum likelihood (Csanády, 2000), obtaining rate estimates r12, r21, r23, and r32. Mean opening and closing rates were then calculated as kCO = r23[r12/(r12 + r21)] and kOC = r32 (Colquhoun and Sigworth, 1995).
For low micromolar [Ca2+]i we mostly used patches in which N (estimated from bracketing segments at 125 µM Ca2+) was >10. Such segments were first fitted assuming a channel number N’ = 10, which returns an unbiased estimate for kOC but overestimates kCO. The correct opening rate was then obtained from the two simple relationships N’Po’ = NPo (where Po’ is the open probability obtained by the cycle-time method assuming N’ channels) and Po = kCO/(kCO + kOC). Thus, kCO = kOCN’Po’/(N − N’Po’).
For [Ca2+]i >40 µM, Po approached unity, and we only studied segments of record with no more than 10 active channels. Because segments with constant N were short due to the rundown, we generalized the method described in Csanády (2000) to an ensemble maximum likelihood fitting of many such short records, using for each segment its own individual N value (the maximum current level in that segment), but a single common value for the four fitted rate constants (Fig. S5, available at http://www.jgp.org/cgi/content/full/jgp.200810109/DC1). Thus, to extract opening and closing rates at 43.6, 125, and 398 µM [Ca2+]i and low extracellular Ca2+ (see Fig. 5, B and C, closed circles) ensembles of 5, 12, and 18 segments were fitted, respectively; whereas 6, 16, and 16 segments, respectively, were fitted to obtain the corresponding parameters at 1 mM of extracellular Ca2+ (see Fig. 5, B and C, open circles).
Fitting TRPM2 gating by the MWC model is a simplification: the 10 states depicted in Fig. 5 D are compound states and the rates printed on the arrows represent mean steady-state rates of transition among these. A clear indication of such further complexity is that even at saturating [Ca2+]i, which should restrict channel gating to the two rightmost states in Fig. 5 D (all black), the distribution of closed event durations contains at least two exponential components. However, the principle of microscopic reversibility also applies to such compound systems regarding ligand concentration dependence of mean opening and closing rates (Csanády and Adam-Vizi, 2004), allowing us to use the MWC model to fit [Ca2+]i dependence of these simple mean parameters.
The MWC model for a channel with n Ca2+-binding sites predicts [Ca2+] dependence of opening rate, closing rate, and Po in the following forms:
(Monod et al., 1965; see Fig. 5 D for notation). The ensemble of the three dose-response curves for Po, kCO, and kOC (Fig. 5, A–C, closed circles), obtained as described in the previous section, were fitted to the above three equations by minimizing the sum of the squared errors for all the data points in the three plots with respect to the five free parameters α, β, f, g, and Kdc (Kdo = (f/g)Kdc). Increasing n values yielded increasingly better fits, but the improvement became negligible for n > 4 (Fig. S6, available at http://www.jgp.org/cgi/content/full/jgp.200810109/DC1).
Section 1 of the online supplemental material describes equations used for calibration of Ca-Green 5N fluorescence (illustrated by Fig. S1) and for determination of the Kd of calcium gluconate (illustrated by Fig. S2); Table S1 summarizes measured free [Ca2+] in our sodium gluconate–based bath solutions without added EGTA. Section 2 describes equations used for calibration of FURA-2 fluorescence (illustrated by Fig. S3); Table S2 summarizes measured free [Ca2+] in our sodium gluconate–based bath solutions containing 1 mM EGTA. Section 3 summarizes testing of our noise analysis algorithm on simulated datasets using four different gating models (illustrated by Fig. S4). Section 4 describes equations used for ensemble maximum likelihood fitting of a large number of short segments of record (illustrated by Fig. S5) and compares the efficiency of this procedure for estimation of rate constants from simulated records containing missed events to that of the cycle-time method. Section 5 shows global fits by the MWC model to the data in Fig. 5 assuming n values ranging from 1 to 10 (Fig. S6). Online supplemental material is available at http://www.jgp.org/cgi/content/full/jgp.200810109/DC1.
Xenopus oocytes have been successfully used for expression and characterization of many ion channels including TRPM8 (Rohacs et al., 2005), a homologue of TRPM2, and were selected for this study. Because TRPM2 requires intracellular Ca2+ for activation and Xenopus oocytes exhibit large Ca2+-activated Cl− currents, we replaced Cl− on both sides of the membrane with gluconate, a bulky anion that does not permeate through endogenous Ca2+-activated Cl− channels (Qu and Hartzell, 2000). Patch pipettes were half filled with a sodium gluconate–based solution and a NaCl-based saline was carefully layered on top to secure a high [Cl−] for the pipette electrode (Fig. 1 A, left). After patch excision, the tip of the patch pipette was placed into a fast perfusion device with continuous flow, connected to the bath electrode via a KCl-agar bridge. This allowed addition/removal of bath chloride without affecting electrode potentials. With Cl− in the bath, addition of Ca2+ to the cytosolic face of patches excised from noninjected oocytes induced large inward currents (Fig. 1 A, left, test a vs. b) which did not reverse (Fig. 1 A, right, black circles), as expected for a Cl−-selective current. In contrast, in symmetrical sodium gluconate, addition of cytosolic Ca2+ failed to induce any endogenous currents (Fig. 1 A, left, test d vs. c, and Fig. 1 A, right, gray circles). We therefore chose to study TRPM2 channels in symmetrical sodium gluconate at a membrane potential of –20 mV (unless otherwise indicated).
In patches excised from oocytes injected with 10 ng of human TRPM2 cRNA, cytosolic exposure to ADPR plus Ca2+ instantaneously elicited large TRPM2 currents, which then started to decay in the maintained presence of both activating ligands (Fig. 1 B). This slow “rundown,” already reported for both human (Sano et al., 2001) and rat TRPM2 (Hill et al., 2006) expressed in mammalian cells, had a time constant of 1–2 min and was neither prevented nor reversed by application of 200 nM CAM (Fig. 1 B), application of 25 µM dioctanoyl phosphatidylinositol 4,5-bisphosphate (not depicted), application of 300 nM of catalytic subunit of protein kinase A + 1 mM ATP (not depicted), or lowering of intracellular [Mg2+] to 200 µM (not depicted).
TRPM2 unitary currents, assayed in patches from oocytes injected with smaller amounts (0.1–1 ng) of cRNA, were linear and reversed at near zero millivolts in both symmetrical Na+ (Fig. 1 C, traces and closed circles) or with Na+ in the pipette and K+ in the bath (Fig. 1 C, open circles). Single-channel slope conductances were 51.3 ± 1.6 pS (n = 6) and 57.1 ± 0.8 pS (n = 5), respectively, under these two conditions, similar to those reported before for TRPM2 expressed in mammalian cells (Perraud et al., 2001; Sano et al., 2001).
We next determined the apparent affinity of macroscopic TRPM2 currents for ADPR by superfusing the cytosolic face of macroscopic patches with increasing [ADPR] in saturating [Ca2+]i. Applications of various test [ADPR] were either bracketed by applications of maximal (32 µM) [ADPR] or executed in a rapid sequence (Fig. 1 D, left) to avoid distortion by rundown. Normalized currents were plotted against [ADPR] (Fig. 1 D, right) and fitted with the Hill equation (solid line). To our surprise, TRPM2 channels were activated by ADPR with a K1/2 of 1.1 ± 0.2 µM, contrasting at least an order of magnitude higher K1/2 values obtained in previous whole-cell studies (Perraud et al., 2001; Sano et al., 2001; Kolisek et al., 2005). Note that 125 µM Ca2+ alone failed to elicit TRPM2 currents, nor was 32 µM ADPR sufficient to maintain TRPM2 activity after Ca2+ removal (Fig. 1 D, left). Thus, Ca2+ and ADPR are both required for TRPM2 activation, which is consistent with an earlier report (McHugh et al., 2003).
Together, these experiments confirm that human TRPM2 is efficiently expressed in Xenopus oocytes yielding channels with properties very similar to those seen in mammalian expression systems.
Before attempting to study the kinetics of gating of single TRPM2 channels we first had to address the kinetic nature of the rundown. To obtain an estimate of Po without any assumptions about the number of channels, we subjected the rundown time courses of macroscopic TRPM2 currents in the presence of saturating [ADPR] and [Ca2+]i (Fig. 2 A) to noise analysis (see Materials and methods and Fig. S4). To this end, current decay time courses (I; Fig. 2 A, black trace) were fitted by single exponentials (Fig. 2 A, red line), and the time course of pure gating noise (Î; Fig. 2 A, blue trace) was isolated by subtracting the fit line from the current. The variance of Î and the mean of I were calculated for consecutive time segments (Fig. 2 A, colored bars), and −σ2(Î)/i was plotted as a function of −m(I) (Fig. 2 B, colored circles). In all cases tested, these plots were reasonably linear and had small slopes. Po values obtained by linear regression through all the points in such plots (Fig. 2 B, black line) were high (Po = 0.92 ± 0.01 [n = 5]), and the majority of the data points fell into the area of the graphs corresponding to Po values >0.8 (Fig. 2 B, gray shaded area). These data indicate that the rundown in excised patches, observed in the presence of saturating [ADPR] and [Ca2+]i, reflects progressive, irreversible inactivation of channels gating with high Po, rather than a graded decline in channel open probability.
A closer observation of patches with smaller numbers of channels (Fig. 2 C) exposed to saturating ligand concentrations (>40 µM [Ca2+]i and 32 µM ADPR) confirmed that the rundown indeed consisted of a progressive irreversible loss of active channels over time (Fig. 2 C, red arrows), while the remaining channels continued to gate with a high Po approaching unity (also see Fig. 4 A, right, expanded segments on bottom). Splitting such records into brief segments in which N could be considered constant (i.e., between two red arrows in Fig. 2 C; N was taken as the maximum number of simultaneously open channels) allowed us to construct stability plots of mean closed time, mean open time, and Po (Fig. 2 D, each bar corresponds to the short segment of time identified in Fig. 2 C by color coding). Such plots did not reveal any strong tendencies in the gating parameters of the surviving channels; the large stochastic variations in mean open and closed times seen in Fig. 2 D rather reflect the small numbers of gating events in each individual segment with constant N. Thus, neither Po nor the kinetics of gating of individual surviving channels is drastically affected by the rundown.
We next determined the apparent affinity of TRPM2 currents for activation by intracellular Ca2+ in the presence of saturating (32 µM) ADPR. With nominally zero (~4 µM) Ca2+ in the pipette solution, we exposed macroscopic inside-out patches to various test [Ca2+]i, bracketed by exposures to our control [Ca2+]i of 125 µM (Fig. 3 A). Fractional currents at each test [Ca2+]i, obtained by normalization to the mean current observed in bracketing 125-µM segments, were plotted as a function of [Ca2+]i (Fig. 3 B, closed circles) and fitted to the Hill equation (solid line) to yield a K1/2 of 22.4 ± 0.9 µM and a Hill coefficient n = 1.62 ± 0.08 (Fig. 3 B). This low apparent affinity and Hill coefficient for TRPM2 activation by Ca2+ in excised patches contrasts an ~50-fold smaller K1/2 and n of ~5 estimated in previous whole-cell studies (McHugh et al., 2003; Starkus et al., 2007). Of note, we could detect only slight changes in the apparent affinity for Ca2+ over the time course of an experiment.
To obtain information about mechanism, we investigated [Ca2+]i dependence of single-channel gating kinetics at saturating [ADPR] (Fig. 4 A), while maintaining extracellular (pipette) [Ca2+] at nominally zero (~4 µM). This was a nontrivial task. First, in the face of the continuous rundown the slow gating of TRPM2 channels hampers collection of sufficient gating events at steady state (see Fig. 2 D). We therefore analyzed patches with multiple channels in which individual gating events were still well resolved (Fig. 4 A, see expanded traces on bottom) but more events could be recorded over shorter time periods. Second, the pattern of single-channel gating is complex, containing at least two populations of closed events. We therefore limited our analysis to extracting, for each [Ca2+]i, mean opening and closing rates, defined as the inverses of the mean closed and open times, respectively. These parameters can be conveniently extracted from multichannel patches, provided that the number of active channels (N) is known (Fig. 4 A and Materials and methods).
Thus, for >40 µM [Ca2+]i we obtained opening and closing rates from a global fit (see Materials and methods) to a large ensemble of short segments of record in each of which N could be considered constant (i.e., between two red arrows in Fig. 4 A, right). In contrast, the low Po activity at low micromolar [Ca2+]i was analyzed in patches with larger numbers of channels (Fig. 4 A, left current trace), and N in such test segments was estimated by linear interpolation between the N values determined for bracketing segments at saturating [Ca2+].
A plot of open probabilities obtained in this fashion yielded a dose-response curve (Fig. 5 A, closed circles) in good agreement with the Ca2+ dependence of macroscopic TRPM2 current (Fig. 3 B, closed circles). Activation of Po by intracellular Ca2+ reflected a large stimulation of channel opening rate (Fig. 5 B, closed circles), whereas closing rates (Fig. 5 C, closed circles) remained at ~1 s−1 and were little influenced by [Ca2+]i in the micromolar range (see the expanded current traces in Fig. 4 A).
If channel closing rate were truly independent of [Ca2+]i, then the rate of decay of macroscopic current should reflect this constant closing rate under circumstances in which the opening rate is zero, e.g., upon sudden removal of intracellular Ca2+. Intriguingly, we invariably observed an ~20 times faster macroscopic current decay upon Ca2+ removal (Fig. 1 D, Fig. 2 A, and Fig. 4 A), suggesting that the closing rate might also be affected by Ca2+, but with an apparent affinity too high for its detection in steady-state records because channels cease to open at submicromolar [Ca2+]i.
To measure closing rate at such submicromolar [Ca2+]i, we resorted to macroscopic concentration jump experiments using a series of bath solutions in which free [Ca2+] was buffered to 8 nM (our “zero-Ca2+” solution), 30 nM, 100 nM, 300 nM, or 1 µM using EGTA. We repeatedly activated macroscopic TRPM2 currents using 125 µM Ca2+, followed by a sudden switch to these test Ca2+ levels (Fig. 4 B). The rate of current relaxation to a new equilibrium, elicited by a sudden change in the rates of gating, is given by the sum of the opening and the closing rate under the new condition. Because opening rates are extremely low at <5 µM [Ca2+], the staircase-like current decay seen in these test segments (see expanded traces in Fig. 4 B, bottom) reflects channel closing rate, given by the inverse of the time constant of a fitted single exponential (Fig. 4 B, top, colored lines, time constants are in milliseconds). Our extended dose-response curve for channel closing rate (Fig. 5 C, closed diamonds) clearly showed a dose-dependent acceleration of channel closure by lowering [Ca2+]i in the submicromolar range. Importantly, for 4.4 µM [Ca2+]i, the concentration jump experiments yielded an estimate of closing rate similar to that obtained from steady-state single-channel analysis.
A simple mechanism by which a ligand can activate a channel is if the binding site has a higher affinity for the ligand in the open than in the closed channel conformation: by microscopic reversibility, ligand binding will then stabilize the open state. Because the apparent affinities for affecting opening and closing rate reflect the ligand-binding affinity of the closed and open channel, respectively (Csanády and Adam-Vizi, 2004), the kinetic results in Fig. 5 (B and C) are compatible with such a mechanism. The fourfold symmetry of homotetrameric TRPM2 channels (Maruyama et al., 2007) implies at least four Ca2+-binding sites, prompting us to consider the MWC model (Monod et al., 1965), a simple application of the aforementioned concept to a multimeric protein. In this model the channel retains its fourfold symmetry at all times (Fig. 5 D): the four subunits transit between open (circles) and closed (squares) conformations in a concerted manner. Ligand binding to individual subunits (Fig. 5 D, black symbols) are independent events and additively affect the energetics of the open-closed equilibrium by virtue of the difference in ligand dissociation constants of the open (Kdo) and the closed channel (Kdc). In essence, each binding event alters the unliganded closing rate (α) and opening rate (β) by some constant factor (f and g, respectively). We performed an ensemble fit (see Materials and methods) of the three dose-response curves for opening rate, closing rate, and Po (Fig. 5, A–C, closed circles) using the model in Fig. 5 D and obtained an excellent fit to all three curves (Fig. 5, A–C, solid lines; predicted midpoints are plotted in each panel). Thus, in the presence of saturating ADPR, activation of TRPM2 by intracellular Ca2+ is well described by the MWC model with the kinetic parameters printed in Fig. 5 D. The fact that the time courses of channel closure are reasonably fitted by single exponentials even in the 100-nM range (Fig. 4 B), where the population of open channels is likely distributed between several different open states (Fig. 5 D, bottom states), suggests that, at least for open channels, Ca2+ binding/unbinding is in rapid equilibrium compared with the rates of channel closure.
Because extracellular Ca2+ strongly potentiates TRPM2 whole-cell currents (McHugh et al., 2003; Starkus et al., 2007), we performed experiments similar to those in Figs. 2 and and4,4, but in the presence of 1 mM of free Ca2+ in the extracellular (pipette) solution. However, extracellular Ca2+ failed to affect fractional activation of macroscopic TRPM2 current by various [Ca2+]i in excised patches (Fig. 3 B, open circles), consistent with the finding of Starkus et al. (2007) in whole cells. Moreover, detailed analysis of steady-state single-channel gating parameters failed to detect any significant effect of extracellular Ca2+ on single-channel Po, opening rates, or closing rates (Fig. 5, A–C, open circles).
Opening rate is a property of the closed channel and is strongly dependent on [Ca2+] bathing the intracellular surface of the channels (Fig. 5 B, closed circles). Because the presence of 1 mM of extracellular Ca2+ does not affect this relationship (Fig. 5 B, open circles) we can conclude that under our experimental conditions, with the cytosolic face of the patch immersed into a rapidly flowing solution, the local [Ca2+] experienced by the activating sites of a closed channel is not influenced by the absence or presence of extracellular Ca2+. This strongly suggests that the activating sites are located on the intracellular side of the channel gate, where as soon as a channel closes, any local elevation in [Ca2+], caused by prior Ca2+ influx through the open pore, is instantaneously (within ~2 µs; see Discussion) eliminated by the rapid vectorial flow of the bath solution.
Closing rate is a property of the open channel but, in contrast to opening rate, TRPM2 closing rate is not sensitive to [Ca2+]i in the micromolar range (Fig. 5 C, closed circles). Therefore, the fact that 1 mM of extracellular Ca2+ failed to affect steady-state closing rates (Fig. 5 C, open circles) does not preclude the possibility that the presence of extracellular Ca2+ might elevate local [Ca2+] around the activating sites of an open channel caused by Ca2+ ions entering through the pore. Such a possibility must be evaluated at some submicromolar [Ca2+]i at which channel closing rate is sensitive to [Ca2+] (Fig. 5 C, closed diamonds).
We therefore examined the rate of decay of macroscopic TRPM2 current, activated by 32 µM ADPR and saturating [Ca2+]i, upon sudden removal of intracellular Ca2+, with 1 mM of free Ca2+ in the extracellular solution (Fig. 6 A). Again, like in the absence of extracellular Ca2+, TRPM2 currents decayed as a staircase, with almost no channel opening events (Fig. 6 A, right trace and blue single-exponential fit line), but the time constant of channel closure was dramatically prolonged compared with that seen in the absence of extracellular Ca2+ (Fig. 6 A, left trace and red single-exponential fit line). On average, 1 mM of extracellular Ca2+ slowed channel closure in 8 nM [Ca2+]i by ~15–20-fold (Fig. 6 A, inset) to the same level observed at steady state in micromolar [Ca2+]i (Fig. 5 C, compare open diamonds and circles).
Thus, even while the cytosolic surface of the channels is immersed into a rapidly flowing Ca2+-free solution, the activating sites of an open TRPM2 channel experience a local [Ca2+] in the micromolar range when Ca2+ is present on the extracellular side. This suggests that the activating sites are not on the protein surface but in a deep vestibule, in the immediate vicinity of the cytosolic mouth of the pore (Fig. 6 B). In the absence of extracellular Ca2+ (Fig. 6 B, top), sudden removal of intracellular Ca2+ results in immediate loss of Ca2+ from the activating sites, followed by channel closure at the fast rate (~20 s−1) characteristic of nonliganded channels (Fig. 5 D, pathway marked by solid arrow). In contrast, when Ca2+ is present on the extracellular side (Fig. 6 B, bottom), Ca2+ ions arrive through the pore as long as the gate is open and maintain a high local [Ca2+] around the activating sites, keeping them saturated and allowing the channel to close at the slow rate of ~1 s−1 characteristic of fully liganded channels (Fig. 5 D, pathway marked by dotted arrow). The fact that these channels, which must have closed in a fully liganded state (to top right state in Fig. 5 D), nevertheless do barely reopen (Fig. 6 A, right trace) indicates that in the absence of Ca2+ in the bath Ca2+ ions are lost from the activating sites much faster than the rate of reopening of a fully liganded channel (estimated ~20 s−1; Fig. 5 B). This provides further support for the notion that Ca2+ binding/unbinding is a rapid equilibrium process compared with the rates of gating.
If the slowing of channel closure by extracellular Ca2+ (Fig. 6 A) indeed requires entry of Ca2+ ions through the pore, then this effect should be dampened by conditions that decrease the electrochemical driving force for Ca2+ influx, e.g., by membrane depolarization. To test this idea, we studied the dependence on membrane potential of the delay in channel closure caused by extracellular Ca2+ (Fig. 7).
Without added Ca2+ in the extracellular solution, in the presence of 32 µM ADPR, similar sized currents (aside from slow continuous rundown) were evoked by brief pulses of 125 µM of intracellular Ca2+ at identical absolute membrane potentials of opposing polarity (Fig. 7 A). More importantly, the rates of channel closure upon rapid removal of intracellular Ca2+ (i.e., upon lowering of [Ca2+]i to 8 nM), obtained as the reciprocals of the time constants of fitted single exponentials (Fig. 7 A, colored solid lines [time constants are in milliseconds]), remained constant between voltages ranging from –80 to +60 mV (Fig. 7 C, black circles). These experiments show that under our experimental conditions TRPM2 channel gating possesses no significant intrinsic voltage dependence.
In contrast, with 1 mM Ca2+ on the extracellular side channel closing rate in 8 nM [Ca2+]i became strongly voltage dependent (Fig. 7 B). The slow decay time constant observed before at –20 mV (Fig. 6 A) was further prolonged at more negative potentials, but became progressively shorter at depolarized potentials (Fig. 7 B, colored solid lines and time constants) approaching those seen in the absence of extracellular Ca2+ (Fig. 7 A). Between –80 and +60 mV depolarization accelerated closing rate by >10-fold (Fig. 7 C, red circles), consistent with the model in Fig. 6 B.
Because EGTA retains two protons at pH 7.1, titration of EGTA with Ca2+ results in H+ release. We therefore considered the possibility that Ca2+ ions entering through the pore might delay channel closure (Fig. 6 B) as a consequence of a local lowering of pH, rather than because of saturation of the activating sites. We repeated our experiments in Fig. 7 B using a zero Ca2+ bath solution made using 1 mM BAPTA (free [Ca2+] ~7 nM). Because BAPTA is completely deprotonated at pH 7.1, a puff of Ca2+ ions into such a solution is not expected to affect local pH. We obtained identical results using either BAPTA or EGTA; the plots in Fig. 7 C contain data using both types of chelators.
The apparent affinity for TRPM2 activation by Ca2+ we have measured in excised patches is >50-fold lower as compared with estimates in previous whole-cell studies (McHugh et al., 2003; Starkus et al., 2007), making us wonder what the reason for this discrepancy might be. Because in those studies much higher ADPR concentrations (0.5 and 1 mM) were used, we tested whether increasing [ADPR] to 1 mM might increase the apparent affinity for Ca2+ activation. However, the presence of 1 mM of intracellular ADPR failed to enhance fractional activation of macroscopic TRPM2 current by 15 µM of intracellular Ca2+ (I15 µM Ca2+/I125 µM Ca2+ was 0.19 ± 0.03 [n = 5]; compare to Fig. 3 B and Fig. 5 A).
Conversely, our K1/2 for TRPM2 activation by intracellular ADPR in the presence of saturating (125 µM) [Ca2+]i (Fig. 1 D) was at least an order of magnitude smaller than previous whole-cell estimates (Perraud et al., 2001; Sano et al., 2001; Kolisek et al., 2005), which were obtained at lower intracellular [Ca2+]. We therefore tested whether K1/2 for ADPR activation would be increased at lower [Ca2+]i. However, lowering [Ca2+]i to ~15 µM did not significantly alter fractional activation by 1 µM of intracellular ADPR, which remained about half-maximal (I1 µM ADPR/I32 µM ADPR was 0.42 ± 0.1 [n = 7]), with 10 µM ADPR still saturating (I10 µM ADPR/I32 µM ADPR was 1.1 ± 0.3 [n = 3]; compare to Fig. 1 D). Thus, in excised patches the apparent affinities of TRPM2 for intracellular ADPR and intracellular Ca2+ are little sensitive to the concentration of the other ligand.
Under whole-cell conditions the sensitivity of TRPM2 current for various activating ligands was shown to be sensitive to the species of intracellular cation used (Beck et al., 2006; Starkus et al., 2007). We therefore tested whether replacing intracellular Na+ with the more physiological K+ might affect the apparent affinity for ADPR and/or Ca2+ activation of TRPM2 currents in excised patches. To this end we alternately exposed macroscopic patches to potassium gluconate–based bath solutions containing various test concentrations or 32 µM ADPR, all in the presence of saturating [Ca2+]i (Fig. 8 A), and normalized the currents in various test [ADPR] to that obtained in the presence of 32 µM ADPR in the same patch. A plot of normalized currents as a function of [ADPR] (Fig. 8 B, closed circles) resulted in a dose-response curve similar to that obtained with intracellular Na+ (Fig. 1 D); a fit to the Hill equation (Fig. 8 B, solid line) yielded K1/2 = 1.0 ± 0.1 µM and n = 1.98 ± 0.5.
Similarly, we exposed macroscopic patches to a potassium gluconate–based bath solution containing various test [Ca2+]i, bracketed by exposures to 125 µM [Ca2+]i, all in the presence of 32 µM ADPR (Fig. 8 C; compare to Fig. 3 A). Plots of fractional current at various test [Ca2+]i yielded dose-response curves very similar to those seen in intracellular Na+ (Fig. 3 B), both when extracellular [Ca2+] was 4 µM (K1/2 = 31.9 ± 3 µM; Fig. 8 D, closed circles and solid fit line) or with 1 mM of free Ca2+ in the pipette solution (K1/2 = 22.8 ± 2.8 µM; Fig. 8 D, open circles and dotted fit line). Thus, in inside-out patches the apparent affinities for ADPR and Ca2+ activation of TRPM2 are little sensitive to whether Na+ or K+ is used as the intracellular cation.
Since its cloning in 1998 (Nagamine et al., 1998), TRPM2 has been in the focus of intensive research, but the majority of these investigations were performed on intact cells, with limited control over the composition of the intracellular solution, and have provided little information on molecular mechanisms. The present study is the first in which TRPM2 channel gating has been scrutinized at the single-channel level in a cell-free system.
The apparent affinities we have found for activation of TRPM2 by both Ca2+ and ADPR greatly differ from those established in whole-cell experiments (but see Lange et al., 2008). We have tested whether differences in our recording conditions could have affected channel activation, but have found that neither our lower [ADPR] nor the use of Na+ as the intracellular cation were responsible (Fig. 8). One possible explanation for the discrepancy could be a potential loss of some regulatory factor upon patch excision. However, a second possible explanation is that whole-cell TRPM2 currents do not saturate at ~1 µM [Ca2+], similar to large-conductance Ca2+-activated K+ channels that are activated in living cells by micromolar Ca2+ from sparks (Rothberg and Magleby, 1999; Pérez et al., 2001). This is because the effect of high micromolar intracellular Ca2+ on TRPM2 whole-cell currents has never been tested and so it cannot be excluded that further activation would have been obtained in such experiments. (Of note, our gating model [Fig. 5 D] also predicts an ~100-fold increase in Po between [Ca2+]i values of 8 nM and 2 µM, because of a decrease in channel closing rate; and the reliability of the “plateau-ing” seen at ~1 µM Ca2+ in whole-cell dose-response curves is somewhat limited by the fact that in such experiments each individual ligand concentration is tested in a different cell.) An example supporting such a possibility is the homologue TRPM4, for which a K1/2 value for Ca2+ activation of 320 nM was estimated from whole-cell current responses restricted to a range of [Ca2+]i between 100 nM and 1 µM (Launay et al., 2002), whereas a K1/2 of 20 µM was obtained for the same channel from whole-cell currents assayed over a range of [Ca2+]i between 100 nM and 100 µM (Ullrich et al., 2005). Finally, it remains a possibility that in intact cells the local ligand concentrations that the channels experience might differ from the bulk cytosolic concentrations and that the concentration of one ligand influences the local concentration of the other, such that these remain poorly defined in whole-cell experiments. As an example, the almost all-or-none type activation by intracellular [ADPR] (nH of ~9) found in whole cells (Perraud et al., 2001) is at large with our findings in inside-out patches (Fig. 1 D) and, if taken at face value, would be hardly explained by any reasonable molecular mechanism. It seems more likely that, through the stimulation of TRPM2 open probability, increasing [ADPR] increasingly elevates local [Ca2+] around the activating sites, yielding apparent positive feedback. Also along these lines, we found no evidence for voltage dependence of gating in symmetrical Na+-based solutions, suggesting that such a phenomenon, reported in whole cells loaded with Na+ (Perraud et al., 2001; Fig. 7 A), was caused by voltage-dependent alteration of some cellular parameter involved in TRPM2 regulation. Thus, although whole-cell studies have outlined TRPM2 as a channel regulated by a multitude of diverse factors in an extremely complex manner, further biophysical studies in cell-free patches will need to sort out which of those factors really act on the TRPM2 protein itself.
Consistent with previous observations (Sano et al., 2001; Hill et al., 2006), TRPM2 activity runs down in excised patches. Although all our efforts to prevent this rundown have so far failed, our detailed kinetic analysis (Fig. 2) revealed that the rundown consists of a progressive irreversible decline in the number of active channels, rather than of a graded decline in open probability. In the face of this technical challenge we have developed a significant collection of novel analytical tools to extract single-channel gating parameters using both noise analysis of macroscopic patches (Fig. 2 and Fig. S4) and ensemble maximum likelihood fitting of sets of dwell-time histograms (Fig. S5). These tools will prove useful for further studies of TRPM2 gating.
Through a combination of steady-state single-channel recordings and macroscopic concentration jump experiments we established [Ca2+]i dependence of single-channel gating parameters, including closing rates in the submicromolar [Ca2+] range in which the channels do not open (Figs. 4 and and5).5). The MWC model, with four postulated Ca2+-binding sites, provides an excellent framework for understanding the dependence of these parameters on [Ca2+]i in the presence of saturating ADPR. Although we cannot exclude the possibility of more than four Ca2+-binding sites (Fig. S5), no more than four sites were required to fit the kinetic parameters of gating (Fig. 4). Our fit parameters suggest that binding of a single Ca2+ ion to an activating site, by virtue of its tighter binding in the open conformation, increases the open-closed equilibrium constant by a factor of ~33 (g/f; see Fig. 5 D), equivalent to an energetic stabilization of the open state by ~3.5 kT or ~8.6 kJ/mol. Ca2+ binding to all four sites activates TRPM2 ~106-fold corresponding to a ~14-kT reduction of the open state free energy. As a comparison, the nicotinic acetylcholine receptor, the best studied ligand-gated channel, contains only two ligand-binding sites, and a comparable overall activation (~108-fold) requires much larger effects (~104-fold) of the two individual ligands (Jackson, 1986).
The rate of our solution exchange, estimated from the rate of decay of endogenous Ca2+-activated Cl− current upon sudden removal of Cl− (Fig. 1 A) was ~50 s−1, less than ~3 times faster than the fastest TRPM2 closing rates we have measured (~20 s−1; Fig. 5 C, closed diamonds). It is therefore likely that this maximal closing rate of unliganded TRPM2 is slightly underestimated. Steady-state analysis of the small number of openings we could collect from segments of record at 8 nM of intracellular and ~4 µM of extracellular Ca2+, from 14 patches with very large numbers of channels, gave an estimate for closing rate of 27 ± 5 s−1. Nevertheless, this potential inaccuracy does not affect the conclusions of our study.
Our experiments have outlined the probable location of the Ca2+-binding sites on the TRPM2 channel responsible for activation of gating. The clear demonstration that these binding sites sense extracellular Ca2+ when the pore is open, but not when the pore is closed (Figs. 5 and and6),6), allows us to assign them with great confidence to a location intracellularly of the gate. This conclusion is further corroborated by our demonstration that membrane depolarization, which slows the rate of Ca2+ entry through the open pore, reduces the effect of extracellular Ca2+ on the activating sites of an open channel (Fig. 7). In contrast, the dramatic slowing by external Ca2+ of channel closure upon removal of intracellular Ca2+ (Fig. 6 A) suggests that Ca2+ ions entering through the pore are able to keep the activating sites saturated as long as the pore remains open (Fig. 6 B, bottom). This is intriguing because, at a molecular level, the rate at which the cytosolic surface of the channels was superfused with Ca2+-free solution can be designated at best as extreme. From the 18-nm diameter of the TRPM2 channel structure (Maruyama et al., 2007) and the ~1-cm/s flow rate of our bath solution we calculate that the entire solution volume in contact with the channel surface is continuously displaced by fresh Ca2+-free solution every ~2 µs. Note that this rate of continuous rinsing is much faster than the rate of solution exchange, which reflects the rate of passage over the patch of a thin layer of solution of mixed composition (requiring ~20 ms; see Materials and methods). We estimate that despite this extremely intensive Ca2+-free surface wash the activating sites of an open channel experience high micromolar [Ca2+] when extracellular Ca2+ is present and the membrane potential is negative (Fig. 5 C and Fig. 7C). These sites must therefore be buried in some deep crevice, shielded from the protein surface, near the intracellular mouth of the pore. The recent, low-resolution electron microscopic reconstruction of TRPM2 revealed a bell-shaped channel structure, consisting of a small compact half-dome pointing toward the extracellular side and a large, sparse intracellular domain containing multiple internal cavities (Maruyama et al., 2007). Based on our functional results we consider these cavities as the most likely candidates for the location of the Ca2+-binding sites responsible for TRPM2 activation.
The broader significance of our dissection of the biophysical properties of single TRPM2 molecules is that these provide important clues for understanding the regulation of TRPM2 activity in intact cells. Although TRPM2 channels are believed to act as coincidence detectors of simultaneous rises in intracellular [ADPR] and [Ca2+], our results suggest that these two ligands regulate TRPM2 with very different dynamics. In our inside-out patches exposed to a flowing Ca2+-free solution activating Ca2+ ions were washed away as soon as a channel closed; hence extracellular Ca2+ alone was insufficient to maintain steady-state gating. In contrast, under the static conditions of an intact cell (even in the presence of strong exogenous Ca2+ buffering!) the local [Ca2+] around the activating sites of a TRPM2 channel, resulting from Ca2+ ions entering through its pore, is unlikely to be completely dissipated before that channel reopens. This allows extracellular Ca2+ alone to maintain steady-state TRPM2 activity (McHugh et al., 2003; Starkus et al., 2007). Moreover, it is likely that channels sense some of the Ca2+ entering through the pores of nearby channels. Thus, in an intact cell, extracellular Ca2+ provides robust positive feedback to TRPM2 activation. Our results suggest that in living cells that contain as little as a few micromolar ADPR a single puff of Ca2+ is sufficient to trigger prolonged, self-sustained TRPM2 activity.
We thank Dóra Mayer for oocyte isolation and injection.
L. Csanády is a Bolyai Research Fellow of the Hungarian Academy of Sciences. This work was supported by Országos Tudományos Kutatási Alapprogramok grant F 68143 to L. Csanády.
Abbreviations used in this paper: ADPR, ADP ribose; CAM, calmodulin, cRNA, complementary RNA; MWC, Monod-Wymann-Changeux.