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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nature. Author manuscript; available in PMC 2009 January 28.
Published in final edited form as:
PMCID: PMC2631661

Chemical glycosylation in the synthesis of glycoconjugate antitumour vaccines


Therapeutic vaccines derived from carbohydrate antigen–adjuvant combinations are a promising approach for cancer immunotherapy. One of the critical limitations in this area is access to sufficient quantities of tumour-associated carbohydrate antigens and glycoconjugate adjuvants. At present, availability of the complex oligosaccharide constructs that are needed for the systematic design and evaluation of novel vaccine formulations relies on de novo chemical synthesis. The use of both state-of-the-art and emerging glycosylation technologies has led to significant advances in this field, allowing the clinical exploration of carbohydrate-based antigens in the treatment of cancer.

The identification of distinct glycoprotein and glycolipid constructs that are overexpressed on the cell surfaces of malignant cells13 has spurred intense research into exploiting these tumour-associated antigens for the development of anticancer vaccines46. Tumour-associated carbohydrate antigens are anchored to the cell surface either by a lipid tail (for example, the glycolipids Globo-H, GM2, GD2 and GD3) or by a protein component (for example, glycoproteins that have an N-acetyl-galactosamine core (GalNAc) oligosaccharide, such as TN, T and sialyl-TN (STN))5. Although glycoprotein and glycolipid carbohydrate epitopes have been used in anticancer vaccine investigations, these molecular subunits typically induce only weak T-cell-independent B-cell (antibody) responses, wherein only a small population of glycolipids might be presented to T cells. However, augmentation of antibody response can be accomplished with three-component vaccine formulations. These constructs involve the covalent conjugation of various carbohydrate antigens to an immunocarrier protein such as keyhole limpet haemocyanin (KLH)7. When this protein is processed and presented by antigen-presenting cells a strong T-cell immune response results, leading to cytokine cascades that increase antibody response, not only to KLH but also to the less immunogenic carbohydrate antigens to which it is attached. Additional immune response potentiation of these antigen conjugates is accomplished through co-administration with an immunological adjuvant such as QS-21 (refs 810). Marked eradication of circulating tumour cells and micrometastases in preclinical models, together with prolonged disease-free periods and survival after primary treatment (such as radiation or surgery), highlighted the early potential of carbohydrate vaccines in cancer immunotherapy8,1113. Several additional three-component anticancer vaccine formulations have since advanced to clinical trials1420, and large randomized, multicentre trials of polyvalent vaccines such as these will soon address the clinical impact of immunization against carbohydrate antigens.

Low expression levels of tumour-associated carbohydrate antigens in tissue cultures of cancer cells, as well as difficulties associated with isolating homogeneous material from such sources, prompted extensive research into the chemical synthesis of these complex molecules as their primary means of access2124. Various synthetic strategies have been developed for the preparation not only of these complex oligosaccharide antigens but also of complex carbohydrate immunoadjuvants. The formidable intricacies of regiochemical and stereochemical control in oligosaccharide assembly establish complex carbohydrate construction as one of the most challenging forefronts in chemical synthesis. Here we present an overview of selected glycosylation processes and strategies applied to the synthesis of tumour-associated carbohydrate antigens (TACA) and immunoadjuvants that show promise in anticancer therapeutic vaccines.

Glycosylation strategies

Acetal exchange

Many of the advances in complex carbohydrate synthesis revolve around methods to form the glycosidic bond, because this is the primary means by which monosaccharide building blocks are assembled into more complex oligosaccharide structures2532. For more than a century, methods developed for glycosylation have overwhelmingly favoured an approach in which the carbohydrate coupling partner contributing its anomeric carbon in the anomeric linkage (glycosyl donor) serves the role of an electrophile. The corresponding coupling partner (glycosyl acceptor) thus functions as the nucleophilic counterpart. This strategy typically relies on the use of a selectively protected glycosyl donor (1, Fig. 1a), which incorporates at its anomeric centre a latent leaving group (Lg). In the presence of a suitable electrophilic ‘activator’ (El+), the anomeric functionality is rendered highly electron deficient (2), thereby allowing anomeric substitution by the nucleophilic glycosyl acceptor (Nu–H) to form the glycoconjugate 3. This acetal exchange process governs most existing chemical glycosylation processes, and is effective for coupling not only with simple nucleophiles but also complex oligosaccharide, peptide and lipid glycosyl acceptors.

Figure 1
Glycosylation methods

One of the more direct approaches for glycosylation involves the class of C1-hydroxy glycosyl donors (4, Fig. 1b), in which an unprotected anomeric hydroxyl is exchanged under a controlled dehydration process. Various dehydrating reagents have proved effective, including cationic metal Lewis acids, sulphonium and phosphonium salts, and activated sulphonyl halides. Although this approach minimizes the number of distinct anomeric derivatizations in the glycosylation protocol, alternative strategies involving functionalization of the anomeric hydroxyl into an isolable glycosyl donor before anomeric activation and coupling are often desirable. Among the earliest latent leaving groups used in chemical glycosylations are anomeric halides (5), wherein glycosyl fluorides, chlorides, bromides and iodides can be prepared and activated for glycosylation with their respective halophilic reagents. Other anomeric latent leaving groups involving oxygen-derived functionalities include trichloroacetimidates (6), phosphates/phosphites (7), esters/carbonates/thiocarbonates (8) and various aryloxy groups (9), all of which can be efficiently activated by the corresponding oxo-, aza- or thiophilic reagents. Also among the anomeric O-derived glycosyl donors is the 4-pentenyl glycoside 10, in which electrophiles with high affinity for π-electrons (such as electrophilic halogen reagents) have proved to be useful selective activators. Like the 4-pentenyl glycosyl donors, glycosyl sulphides and sulphoxides (11) have been shown to be exceedingly useful not only as latent leaving groups but also as stable anomeric protective groups before the glycosylation event.

Glycal oxidation

In addition to carbohydrate donors with anomeric heteroatom derivatives, the use of glycals (12, Fig. 1c) as glycosyl donors has been explored extensively in complex carbohydrate synthesis. The presence of the 1,2-alkene functionality in this substrate allows the use of various electrophilic oxidants (El+) that are reactive to enol ether nucleophiles (12). The resulting activated glycosyl donor 13 is then poised to receive an appropriate nucleophilic glycosyl acceptor (Nu–H) to form the glycoconjugate 14. These methods allow the introduction of various functionalities ‘Z’ at the C2-position in conjunction with anomeric bond formation. For example, C2-oxygen transfer to glycal donors has proved useful, involving dimethyldioxirane (DMDO)-mediated33 or sulphonium-mediated34 1,2-epoxidation of glycals, followed by anomeric substitution, to generate C2-hydroxy glycosides (15, Fig. 1d). Similarly, I(iii)-containing reagents have recently been applied to the generation of selectively protected C2-acyloxyglycosides (16)35. The synthesis of 2-amino-2-deoxyglycosides has drawn considerable attention owing to the abundance and importance of this class of glycoside in naturally occurring glycoconjugates. The venerable C2-azidonitration reaction of glycals36 to afford C2-azido pyranose derivatives (17) has been, and continues to be, a favoured method by which to introduce the C2-N-functionality. Other reactions have subsequently been developed for C2-nitrogen transfer onto the glycal donor, including stereoselective installation of a sulphonamide group (for instance, 18, by iodosulphonamido glycosylation)33, as well as less-used protocols to install a C2-carbamate functionality37 and the naturally occurring C2-acetamido group38.

This series of acetal exchange couplings and glycal glycosylations (Fig. 1) has been used, as have others, in the chemical synthesis of highly complex oligosaccharide conjugates. However, so far no single coupling method has proved to be broadly effective for all glycosylations, no doubt as a result of the high structural and functional group variability of carbohydrate substrates. This is the case for the exceedingly complex glycoconjugates identified for potential use in cancer immunotherapy, in which chemical glycosylation processes used to prepare these molecules are often highly substrate-specific.

Synthesis of tumour-associated carbohydrate antigens


Globo-H (19, Fig. 2a) is a cell-surface glycosphingolipid expressed on a number of epithelial tumours, including those of the breast, prostate and ovary3941. Its complex hexasaccharide has been a target of many total synthesis approaches. The DMDO-mediated glycal assembly method33 was elegantly applied to the first total synthesis of a Globo-H oligosaccharide42,43 (Fig. 2b). This approach is illustrated by the stereoselective α-epoxidation of galactal 20 followed by regioselective C3-O-glycosylation of galactal diol 21. The resulting glycal disaccharide 22 is then immediately poised for glycosylation with a selectively protected fucose donor to afford the glycal trisaccharide 23. Synthetic incorporation of 2-amino-2-deoxyglycoside derivatives often presents a challenge in complex carbohydrate synthesis, wherein stereoselective nitrogen-transfer to glycal substrates has proved effective. In this late-stage transformation, activation of the glycal enol ether in 23 with iodonium di-sym-collidine perchlorate (I(coll)2ClO4) generates a transient β-iodonium intermediate that rapidly receives a PhSO2NH2 nucleophile at the anomeric position. The resulting glycosyl sulphonamide group is then transferred to the C2-position in the presence of a base (lithium hexamethyldisilazide, or LHMDS) through the generation of a putative aziridine intermediate that is subsequently opened by ethane thiol. The resulting trisaccharide 24, which incorporates the requisite protected C2-aminogalactoside donor, is ready for thioglycoside coupling with an appropriate tri saccharide acceptor (ROH) to afford the hexasaccharide 25, which can be readily elaborated to the Globo-H antigen (19).

Figure 2
Selected syntheses of tumour-associated Globo-H antigen hexasaccharide

Subsequent syntheses of the Globo-H oligosaccharide used the concept of orthogonal glycosylation. By capitalizing on differences in reactivity of various anomeric leaving groups, in conjunction with the control of nucleophilicity of the acceptor, a convergent synthesis of the Globo-H hexasaccharide was accomplished (Fig. 2c). From five distinct carbohydrate building blocks (2630) with either an anomeric thioalkyl, thioaryl or fluoride functionality, sequential chemoselective anomeric activations were conducted in an orthogonal two-directional glycosylation approach44. For example, the ethylthio fucoside donor 26 could be selectively activated in the presence of the less reactive anomeric phenylthio group of the acceptor 27. After this initial glycosylation of 27 with 26, the remaining anomeric phenylthio group was activated (with N-iodosuccinimide (NIS) in the presence of the galactosyl acceptor 28, whose anomeric fluoride group remained unreactive to the thiophilic NIS reagent. The resulting glycosyl fluoride trisaccharide was then used as the donor for the glycosylation of the remaining Galα1-4Galβ1-4Glc trisaccharide fragment (where Gal denotes galactose and Glc denotes glucose) itself obtained from the glycosylation of the protected lactose acceptor 30 with the thiogalactoside 29. In the late-stage convergent step, fluoride activation (by biscyclopentadienyl zirconium dichloride (Cp2ZrCl2) and silver trifluoro methanesulphonate (AgOTf)) with concomitant triethylsilyl (TES) deprotection allowed stereoselective coupling of both trisaccharide fragments to provide the protected hexasaccharide 31, which served as a suitable advanced substrate for the completion of the synthesis. Notably, no anomeric protecting group interconversions were necessary after any of the glycosylation steps in the synthetic sequence.

The orthogonal reactivity concept was exploited on a different dimension in the reactivity-based one-pot strategy45 for the construction of Globo-H hexasaccharide46 (Fig. 2d). This effort relied on known relative reactivities of various thioglycoside donors, the reactivities of which were finely tuned by the careful choice of proximal protective groups47. The synthesis was initiated through the use of three distinct monosaccharide donors (3234), all of which incorporated the anomeric thiotoluyl latent leaving group. When this mixture of thioglycosides was treated with electrophilic activators (trifluoromethanesulphonic acid (TfOH) and NIS), the electron-rich thioglycoside donor 32 was activated most rapidly to condense with the most nucleophilic alcohol acceptor 33. Subsequent glycosylation of 34 with the remaining anomeric thiotoluyl group on the resultant disaccharide in the one-pot reaction generated the trisaccharide 35 after selective removal of the laevulinate (Lev) protective group. A similar one-pot multiple glycosylation process was applied to a mixture of the trisaccharide 35, the fucosyl donor 36, and the lactose-derived acceptor 37, securing the hexasaccharide 38 for eventual advancement to the Globo-H hexasaccharide. This one-pot strategy resulted in the assembly of the hexasaccharide core with minimal isolation and purification of oligosaccharide intermediates48. Other notable syntheses of Globo-H have involved exclusive application of glycosyl trichloroacetimidates49 and glycosyl phosphates50, demonstrating the power and versatility of these classes of glycosyl donor.

Sialylated gangliosides

Sialylated gangliosides, exemplified by GM2 (39), GD3 (40), and GD2 (41) (Fig. 3a), are cell-surface glycolipids expressed in a number of neuroectodermal cancers (including melanoma, neuroblastoma, sarcoma and small-cell lung cancer) and also, in the case of GM2, in several epithelial cancers (breast, prostate, ovary and colon)3,51. In addition to the typical challenges associated with the chemical synthesis of glycolipids, an added difficulty is the incorporation of sialic acid residues such as neuraminic acid (NeuAc) into complex oligosaccharides52. Glycosylations with sialic acid donors are often plagued by low yields, because anomeric couplings must occur at the sterically encumbered C2-position through a ketal exchange process. Moreover, naturally occurring sialosides incorporate the α-C2-stereochemical configuration, which is the contra-thermodynamic equatorial isomer, devoid of electronic stabilization through anomeric effects. Anomeric leaving groups in sialylation reactions52 have taken the form of thio-derivatives, halides and phosphites, as well as, to a lesser extent, sialic acid-2,3-glycals and the underivatized hemiketal53. Efforts to control α-anomeric selectivity in these couplings have used removable neighbouring auxiliary groups (either at C3 or at C1), or beneficial solvent participation effects in the anomeric substitution event.

Figure 3
Selected syntheses of tumour-associated sialylated glycosphingolipid oligosaccharides

An early synthesis of the GM2 (39, Fig. 3b) antigen made effective use of the β-sialyl phosphite donor 42 in the regioselective sialylation of the C3-hydroxyl of the lactoside acceptor 43 (ref. 54). By capitalizing on the use of nitrile solvents to effect α-sialylations, presumably through a transient β-nitrilium intermediate5557, Sn(ii) catalysed glycosylation in acetonitrile provided the α-sialoside 44 in good yield and α-stereoselectivity. Attachment of the remaining protected galactosamine residue 45 was accomplished with the trichloroacetimidate glycosylation28 of the lone hydroxyl group in 44 to provide the tetrasaccharide 46, which was subsequently converted to GM2 (39) by straightforward protective group manipulations.

Further enhancement of reactivity in sialic acid glycosylations is evident in a synthesis of GD3 (40, Fig. 3c), wherein the C5-acetamido functionality in neuraminic acid is replaced with a C5-trifluoroacetamido group in the glycosylation substrates (such as 47)58. With this modification, construction of the NeuAcα2-8NeuAc linkage was possible without the need for installation of neighbouring auxiliary groups. Thus, treatment of the thiosialoside donor 47 with NIS in acetonitrile solvent allowed α-selective C8-O-glycosylation of the sialyl acceptor 48, also derivatized with the C5-trifluoroacetamido substituent. The beneficial effects of these modified amide substrates in both 47 and 48 are thought to arise from the improved reactivity in 47 as a more electron-deficient electrophilic donor and from the attenuation of a putative C5-amido-C8-hydroxyl hydrogen bond in the acceptor 48 that might otherwise compromise its role as a nucleophile. The attainment of 49 allowed elaboration to GD3 (40), after glycosylation of an appropriate lactoside acceptor and facile late-stage exchange of the trifluoroacetamide groups to their native acetamido counterparts.

Mucin-associated glycans

Mucin motifs make up another well-studied class of tumour-associated carbohydrate antigen59. Mucins are high-molecular-weight glycoproteins expressed on the surfaces of many epithelial cells that are characterized by the presence of GalNAc moieties on the hydroxyl side chains of Ser and Thr residues (which are often clustered) in the protein60. Further glycosyl transferase-controlled extension of the carbohydrate moiety results in the formation of oligosaccharides, which, among other functions, provide protection from proteolytic degradation and microbial infection. As a result of altered glycosyltransferase expression in tumour cells, premature termination of oligosaccharide biosynthesis leads to the formation of shortened, often sialylated, saccharide antigens as well as the exposure of peptide epitopes. Examples of tumour-associated carbohydrate antigens (Fig. 4a) include TN (50), T (51), STN (52) and 2,6-ST (53)10,61,62. A number of these tumour-associated structural alterations represent a basis for the design of vaccines for selective eradication of tumours.

Figure 4
Mucin-related tumour-associated carbohydrate antigens

The chemical synthesis of mucin-derived TACAs involves a glycosylation challenge beyond carbohydrate–carbohydrate coupling: that of the construction of the linkage at the interface between the saccharide and the mucin-derived peptide. Formation of the GalNAcα1-O-Ser/Thr connection (Fig. 4a) highlights one of the key difficulties in achieving C1,C2-cis-glycosylation. Indeed, a C2-acetamido group within a carbohydrate donor is overwhelmingly biased, through oxazoline intermediate formation, to afford the C1,C2-trans glycoside63, a linkage with the anomeric configuration opposite to that of the mucin-related tumour-associated antigen.

To address this challenge, access to the GalNAcα1-O-Ser/Thr substructure is often accomplished through the glycosylation of the side-chain hydroxyl group of protected Ser or Thr derivatives with C2-azido donors (56; Fig. 4b), in which the non-participating nature of the azide substituent allows the stereoselective formation of the desired α-anomer. 2-Deoxy-2-azidogalactopyranose derivatives (55), obtained by azidonitration36 of protected galactal substrates (54), can be converted to various glycosyl donors (56), including anomeric bromides and chlorides64,65, trichloroacetimidates66, thioglycosides67,68 and pentenyl glycosides69, all of which can be used for Ser/Thr glycosylation to generate the carbohydrate–amino-acid conjugate 58. Importantly, these couplings are equally amenable to the construction of oligosaccharide–Ser/Thr conjugates66,7072. After amino-acid glycosylation, the C2 azido group in 58 is converted, through reductive acetylation, to the naturally occurring acetamide group in 59 to allow subsequent use in iterative peptide synthesis.

A conceptually distinct approach (Fig. 4c) to the construction of mucin-type amino-acid–carbohydrate linkage involves the formation of the critical sugar–amino-acid bond from the potassium tert-butoxide-promoted conjugate addition of protected Ser and Thr nucleophiles (61) to a 2-nitrogalactal donor (60)73. Subsequent C2-nitro reductive acetylation and protective-group modification in 62 results in the preparation of glycosylated building blocks 63, which can be readily elaborated for glycopeptide synthesis.

Once formed, the suitably protected glycosylated amino acids can be incorporated into a growing peptide chain in a modular fashion, allowing the preparation of clustered carbohydrate antigen displays, a motif common to tumour cell surfaces. Various oligopeptide–tumour antigen conjugates have been prepared in this manner, including TN antigen constructs74,75, STN antigen glycopeptides64,76, and 2,3- and 2,6-ST conjugates68,77.

Synthesis of carbohydrate immunoadjuvants


A third component that is required in clinically viable anticancer vaccines is a potent immunological adjuvant, a substance that is itself non-immunogenic but that significantly augments the immune response when administered together with the antigen–carrier conjugate. Among the most potent molecular immunological adjuvants used in antitumour vaccines is QS-21A (ref. 9; Fig. 5), a plant-derived complex saponin from the South American tree Quillaja saponaria Molina. Microgram quantities of this amphiphilic substance in combination with the antigen–carrier conjugate lead to enhancement in both antibody and cell-mediated immune response in a host of promising anticancer and antiviral vaccines78. The structure of QS-21A (Fig. 5a) is that of a complex triterpene–oligosaccharide–normonoterpene conjugate, incorporating quillaic acid as a central lipophilic core flanked by a branched trisaccharide, a linear tetrasaccharide, and an extended glycosylation diester side chain along its periphery. QS-21A has two constitutional isomers, in which the terminal saccharide residue on the linear tetrasaccharide substructure is either d-apiose (QS-21Aapi, 64) or d-xylose (QS-21Axyl, 65)79. Unfortunately, acquiring sufficient quantities of these natural products in pure form is fraught with technical challenges, because they exist in a mixture of more than 100 distinct amphiphilic congeners that are typically only partly purified by repeated high-performance liquid chromatography separation.

Figure 5
Immunological adjuvant QS-21A

The first report of the chemical synthesis of both oligosaccharide cores of the natural product showcased expert control in stereoselective glycosylation in the synthesis of fully protected versions of both the branched trisaccharide fragment and the linear tetrasaccharide components of QS-21Aapi (64)80. Two glycosylation methods were used: NIS activation of thioglycoside donors and trimethylsilyl triflate activation of trichloroacetimidate donors. Because all of the glycosidic linkages in 64 are composed of the 1,2-trans-linked relative configuration, anomeric stereocontrol was effected through liberal use of C2-ester protective groups that capitalize on neighbouring group participatory effects81.

More recently, a completed synthesis of QS-21Aapi (64) was accomplished82 (Fig. 5b) that relied on minimal use of C2-ester protective groups to avoid potential difficulties in late-stage selective ester deprotection in the presence of the hydrolytically labile C4-O-fucosyl ester side chain78 in 64. In this effort, five of the glycosidic bonds in 64 were constructed with the sulphoxide-mediated dehydrative glycosylation (Ph2SO·Tf2O) using hemiacetal donors83. It is also worth noting that recently developed oxidative glycosylation protocols were applied in the construction of advanced monosaccharide substrates. For example, I(iii)-mediated oxidative bis(C1,C2-acyloxylation)35 of tribenzylgalactal (66) served to install benzoate groups stereoselectively at both the C2 and C1 positions, with the latter being selectively removed by aminolysis to afford the hemiacetal 67. Thus, neighbouring group participatory effects were bestowed on this donor (67) in the subsequent glycosylation, yet the need for excessive ester protective group exchanges in the late stages of the synthesis was minimized. Ph2SO-mediated dehydrative glycosylation of the β-glucuronic acid (β-GlcA) derivative 68 provided disaccharide 69, which was advanced to the branched trisaccharide trichloroacetimidate 70. The stereoselective glycosylation of the quillaic acid triterpene (QA–OH) with trisaccharide 70 proved to be among the most difficult to secure, owing to the significant steric demands at the attachment sites of both coupling partners. Nevertheless, the desired β-glycosidic linkage in 71 was constructed through the use of the less common B(C6F5)3 Lewis acid as the catalyst84 in the trichloroacetimidate glycosylation. The carbohydrate–triterpene conjugate 71 was then advanced to the natural product 64 by way of late-stage conjugation to protected forms of the linear tetrasaccharide fragment and glycosylated acyl side chain, each of which was prepared by de novo multistep synthesis. In vivo immunological evaluation of synthetic QS-21A in vaccine formulations is currently in progress.


Advances in chemical glycosylation have allowed organic synthesis to fulfil its role as a supplier of rare carbohydrate antigens identified as potential targets for cancer immunotherapy6,20. Although several classes of antigen (for example, GM2, fucose-GM1, GD2 and GD3) can be obtained from natural sources in acceptable quantities, the acquisition of sufficient amounts to investigate others (such as Globo-H, STN, TN and T) still relies on chemical synthesis. However, chemical synthesis has evolved far beyond this initial role, as it also makes it possible to design novel versions of otherwise inaccessible antigen and adjuvant constructs, molecules that might hold the key to overcoming critical challenges in the induction of potent yet selective cellular and humoral responses.

Pursuit of non-natural structural modification of carbohydrate antigens that forcibly enhance the non-self identity of specific oligosaccharide conformations has shown promise, although guidelines for such designs are often empirically derived. For example, synthetic TACAs in the form of sialic acid lactone derivatives of GD2 (ref. 15) and GD3 (ref. 14), or N-propionylated derivatives of polysialic acid14,15,85, show potent serological response in immunized patients compared with their ‘natural’ unmodified counterparts. Moreover, as transformed cells have varying degrees of heterogeneity in the type and distribution of antigens on their surfaces86, it is postulated that polyvalent constructs of various antigens could serve as a better mimic of cancer cells19. An approach to incorporate controlled epitope heterogeneity within homogeneous antigen constructs is exemplified by recently prepared pentavalent neoglycopeptides87, whose KLH conjugates have shown encouraging antibody responses in preclinical evaluations in mice.

Further advances on these fronts will probably entail modulation of epitope selection or spacing in clustered constructs, as well as the development of novel molecular platforms for antigen display8890. These efforts will rely not only on the development and application of new glycosylation technologies but also on innovations in chemoselective ligation reactions for access to increasingly complex and diverse immunogenic molecular arrays. However, chemical synthesis and modification of the immunological adjuvant component is a much less explored area in synthetic vaccine development91,92. Indeed, there have been only limited investigations regarding the chemical modification of QS-21A adjuvant on the basis of natural product degradation and derivatization93,94, providing the first glimpses into its structure–activity relationship profile. The recent synthesis of QS-21Aapi will undoubtedly allow the role of chemical synthesis to extend beyond the arena of antigen construction to that of molecular adjuvant design in the preparation of new conjugate anticancer vaccines.


We thank P. O. Livingston for assistance in proof-reading this article. Research on carbohydrate synthesis in the laboratory of D.Y.G. is supported by the National Institutes of Health.


The authors declare no competing financial interests.


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