Neuroprotection for ischemic brain injury has emerged only recently as a topic of serious biomedical inquiry. A MEDLINE survey (PubMed, 2007
) reveals virtually no publications on this topic until the early 1990’s but a remarkable surge in publications over the past 10 years (). In the last 6 years alone, over a thousand experimental papers and over 400 clinical articles have appeared on this subject.
Figure 1 Numbers of MEDLINE-indexed publications in neuroprotection in stroke / ischemia from 1965 to the present (PubMed, 2007).
The ability of neuroprotection to flourish as a productive field of research depended upon the emergence of a corpus of experimental investigations, beginning in the 1970’s, that defined and characterized the pathophysiology -- i.e., the mechanisms and mediators -- of ischemic brain injury and, by implication, pointed the way to potential interventional strategies for thwarting these injurious factors. Reproducible, physiologically controlled animal models of ischemic injury as well as in vitro systems were developed and validated. The cytopathology of ischemic injury was characterized. Biochemical and molecular events were elucidated, intracellular mediators identified, and numerous important modulatory influences explored. summarizes these key topic-areas and provides useful references. Taken together, these advances in our understanding provided the fertile milieu in which ischemic neuroprotection could be rationally approached.
Overview of ischemia-pathophysiology: topics and targets of neuroprotection
We define neuroprotection as any strategy, or combination of strategies, that antagonizes, interrupts, or slows the sequence of injurious biochemical and molecular events that, if left unchecked, would eventuate in irreversible ischemic injury. This operational definition excludes those therapeutic strategies that are directed primarily at the cerebral vasculature
and have as their goal the maintenance of circulatory patency or the reversal of vascular occlusion. This latter category comprises thrombolytic agents (e.g., tissue plasminogen activator, urokinase, mechanical devices), anti-thrombotic agents (e.g., heparin, low molecular weight heparin), anti-platelet drugs (e.g., aspirin, dipyridamole, abciximab), and fibrinogen-depleting agents (e.g., Ancrod). Admittedly, these agents protect the brain but do so primarily via hemodynamic rather than metabolic mechanisms. Among clinical trials for ischemic stroke, those involving thrombolytic, anti-thrombotic, and anti-platelet agents are by far more numerous than clinical trials of neuroprotectants () (Internet Stroke Center, 2007
), but they will not be further considered here.
Categories of neuroprotectants
provides an overview of categories of neuroprotective strategies that have progressed to some stage of clinical trial (Internet Stroke Center, 2007
). The heuristic value of this graphic is in directing the reader’s attention to the enormous variety
of agents and strategies that have received clinical scrutiny – each grounded to some extent in a pathophysiological rationale. In all, approximately 165 ongoing or completed clinical trials are represented (Internet Stroke Center, 2007
). By contrast, the MEDLINE database contains over 1,300
reports of ischemic neuroprotection in experimental
preparations (Internet Stroke Center, 2007
). The task of extracting a coherent viewpoint from this plethora is daunting.
As recently pointed out, a common view of neuroprotection research is that “everything works in animals but nothing works in people.” (O'Collins et al., 2006
). Following the announcement of unsuccessful outcomes of clinical trials in neuroprotection, it is common for nihilistic generalizations to appear in print that assert or imply that ischemic neuroprotection may not be an attainable clinical goal. It is useful, therefore, to call attention to numerous studies in experimental animals that provide convincing proof-of-principle that high-grade protection of the ischemic brain is indeed achievable. offers a few salient examples, some from the author’s laboratory, assembled to make this point that, in fact, certain therapeutic approaches have proven highly neuroprotective in well-constructed animal studies. Some caveats, however, must be added: It is certainly not the case that “everything works in animals.” It is apparent from the comprehensive review of O’Collins et al (O'Collins et al., 2006
) that the pre-clinical evaluation
of many agents that were subsequently brought to clinical trial showed only modest or inconsistent tissue protection. Among 66 agents (other than thrombolytics and anti-thrombotics) that they considered, the mean (± SD) overall extent of tissue protection for the group was a modest 31 ± 19% (O'Collins et al., 2006
). In addition, there was great variability in the quality or scope of these pre-clinical studies as reflected in their adherence to the Stroke Therapy Academic and Industry Roundtable (STAIR) guidelines (STAIR, 1999
). Thus, many agents were brought to clinical trial without a sufficiently solid or relevant evidence-based pre-clinical foundation. Others have analyzed the shortcomings identified in pre-clinical evaluations of putative neuroprotectants (Gorelick, 2000
; Cheng et al., 2004
). A great number of variables come into play in the design and execution of experimental cerebral-ischemia investigations and may influence the quality, consistency, and outcome of these pre-clinical studies; these are summarzied in .
Proof-of-Principle: Selected examples of high-grade neuroprotection in experimental cerebral ischemia
Factors influencing experimental neuroprotection studies
Clinical trials of neuroprotection for ischemic stroke have been surveyed in recent reviews (Cheng et al., 2004
; Labiche et al., 2004
). The Internet Stroke Center website provides a comprehensive clinical stroke-trial database (Internet Stroke Center, 2007
). and summarize the current universe of neuroprotection trials in acute ischemic stroke (Internet Stroke Center, 2007
). Of the ~160 such trials, one-quarter are currently ongoing and three-quarters have been completed (or prematurely terminated). It is instructive to consider the completed trials in greater detail. Eighty completed studies are, in fact, merely early-phase
safety or feasibility trials with 200 or fewer subjects (). Only 40 completed trials have enrolled over 200 subjects, and of these fewer than one-half have instituted neuroprotective treatment within the 4–6 hour therapeutic window within which effacicious neuroprotection is considered possible. This fact alone is sufficient to explain the abundance of non-successful (“failed”) clinical trials.
Space does not permit a close analysis of all 40 larger completed trials. Rather, greater attention will be focused on the most extensively evaluated agents and classes. For these latter, we shall consider both the pre-clinical evidence (or lack thereof) supporting potential neuroprotective efficacy and the results and shortcomings of the clinical trials themselves. summarizes these trials.
Larger completed and published randomized clinical trials of neuroprotection in acute ischemic stroke
Calcium Channel Blocker: Nimodipine
Calcium plays a central role in the pathophysiology of ischemic brain injury as well as cardiac disorders (Siesjo et al., 1989
; Choi, 1995
; Tymianski et al., 1996
; Erecinska et al., 1996
). Thus, calcium channel antagonists have found applications in the therapy of angina pectoris, hypertension, and certain arrhythmias (Scriabine et al., 1989
). In the brain, blockade of slowly activating (L-type) calcium channels reduces calcium entry into neurons – a potentially salutary action in ischemia and other disorders. Nimodipine is a 1,4-dihydropyridine calcium channel antagonist that exerts neuronal effects and, in addition, is a preferential vasodilator of cerebral vessels, with less pronounced peripheral vasodilation (Kazda et al., 1982
; Scriabine et al., 1989
). In patients with subarachnoid hemorrhage, nimodipine reduces severe neurological deficits due to cerebral vasospasm (Allen et al., 1983
Pre-clinical ischemia studies
Over 250 animal studies of nimodipine in cerebral ischemia have been published. A careful survey of this literature, however, was able to identify only 20 controlled animal studies of focal cerebral ischemia in which nimodipine was administered after
the induction of ischemia and its consequences assessed (Horn et al., 2001b
). Ten of these studies reported a positive outcome, but 10 did not; in the 7 studies in which exact infarct-size data were presented, the pooled effect size (standardized mean difference) favoring nimodipine was −1.2 (Horn et al., 2001b
). In several of the positive studies, nimodipine treatment was initiated within the first 15 minutes
after onset of ischemia. Subgroup analysis of studies in which treatment was initiated at 1 hour or longer after induction of ischemia revealed overall non-significance (Horn et al., 2001b
). The general quality of the studies analyzed was judged to be methodologically weak.
Clinical stroke trials
The Internet Stroke Center registry (Internet Stroke Center, 2007
) lists 13 completed clinical trials of nimodipine in acute ischemic stroke, but 8 of these are early-phase, non-definitive studies of fewer than 200 patients. The largest of these was a double-blind randomized trial of 186 patients treated within 24 hours of stroke onset; during the 4-week treatment period, all-cause mortality was reduced with nimodipine, but the effect was restricted to men (Gelmers et al., 1988
Five larger randomized, double-blind efficacy trials of nimodipine in acute ischemic were subsequently published (). In the first, 1,215 patients were randomized to oral nimodipine or placebo started within 48 hours (TRUST Study Group, 1990
). The trial was negative for the primary end-point – independence at 6 months on the Barthel index (OR 1.22 (95% CI 0.95–1.57)) -- but there was a suggestion of delayed recovery in the nimodipine group at 3 weeks. In the second trial, 1,064 patients were randomized to oral therapy begun within 48 hours and continued for 21 days (American Nimodipine Study Group, 1992
). The primary outcome was defined using the Toronto Scale and a motor strength scale. No primary-outcome difference in mortality or neurological outcome was found with any of the rating scales for the overall cohort, but a post-hoc subgroup analysis suggested benefit in the 120-mg nimodipine group when treatment was begun within 18 hours. In the third trial, 350 patients were also randomized to oral treatment within 48 hours (Kaste et al., 1994
). The primary endpoints were the Rankin scale, neurological score, and mobility at 12 months. No difference between treatment groups was found for the primary-outcome measures, and post-hoc analyses failed to identify any subgroups that benefited. Case-fatality rates were high in nimodipine-treated patients at 1 and 3 months but not at 1 year; this was possibly attributed to the drug’s blood pressure-lowering effect. A fourth trial randomized 295 patients at 34 centers to treatment within 24 h of stroke onset with intravenous
nimodipine or placebo for 5 days. Patients treated with nimodipine, 2 mg/h, fared worse
than the placebo group on the primary outcome measures -- neurological status on the Orgogozo scale and functional status on the Barthel index at 21 days; a similar trend was noted in the lower-dose nimodipine group (Wahlgren et al., 1994
). A meta-analysis published in 1994, using data from these and other, smaller ischemic-stroke trials found no overall benefit of nimodipine; however, in a subgroup of 616 patients treated within 12 hours after stroke onset, a beneficial effect on neurological and functional was reported (Mohr et al., 1994
). Another meta-analysis of calcium antagonists in 825 patients with acute ischemic stroke was unable to confirm this positive result (Horn et al., 2000
). In that analysis, intravenous
administration of calcium antagonists was, in fact, associated with an increase
in poor outcomes; and oral nimodipine started within 12 hours conferred no benefit.
This VENUS Trial, which followed upon these meta-analyses, was intended to test the efficacy of early
nimodipine therapy; 454 patients were randomized to oral nimodipine or placebo in a primary-care setting, with treatment begun within 6 hours and continued for 10 days (Horn et al., 2001a
). The steering committee terminated the trial prematurely owing to sample-size concerns. The primary end point, defined as poor outcome -- death or dependency after 3 months – did not differ between treatment groups. (Horn et al., 2001a
Nearly 3,400 patients were studied in the 5 larger trials summarized here, and over 7,500 in the Cochrane Database meta-analysis (Horn et al., 2000
). These trials, carried out in an era of less sophisticated clinical-trial methodology, evince serious design-flaws. Chief among these is the initiation of treatment in 4 of the 5 major trials at 24 or 48 h after stroke onset, well beyond the likely window of therapeutic efficacy. In several studies, the sample size was unrealistically below the number needed statistically to reveal a modest treatment effect and to avoid a type II error. The inclusion criteria and outcome measures used are also open to criticism. Furthermore, these trials were conducted without adequate attention to emerging pre-clinical data that revealed only limited and inconsistent neuroprotection with nimodipine. The uniformly negative primary outcomes of the clinical trials are thus not surprising.
It is well established that glutamate, the major excitatory CNS neurotransmitter, is also capable of inducing excitotoxic neural injury in the setting of cerebral ischemia and other disorders (Rothman et al., 1986
; Choi et al., 1990
; Benveniste, 1991
; Hossmann, 1994a
; Hansen, 1995
; Ginsberg, 1995
; Pellegrini-Giampietro et al., 1997
; Billups et al., 1998
; Bruno et al., 2001
; Aarts et al., 2003
; Schurr, 2004
; Arundine et al., 2004
). Glutamate and related excitatory amino acids interact with several receptor-classes, which are relevant to neuroprotection. These include the N-methyl-D-aspartate (NMDA) and the 3-hydroxy-5-methyl-4-isoxazole proprionic acid (AMPA) receptors.
Non-competitive NMDA antagonism
The NMDA receptor complex, which mediates both calcium and sodium ion fluxes, contains an agonist-binding site, a glycine modulatory site, and binding sites within the ion channel, where magnesium exerts a voltage-dependent block (Ginsberg, 1995
Pre-clinical ischemia studies
The most extensively studied NMDA antagonist is the non-competitive agent MK-801 (dizocilpine), which binds to NMDA receptors with high affinity and produces a long-lasting blockade. MK-801 was extensively evaluated in animal models of focal ischemia (reviewed in (Ginsberg, 1995
)) and was capable of reducing infarct volume substantially in several models; typically, it was most effective with treatment initiated prior to
ischmia or within the first 1–2 hours thereafter. Dextromethorphan, a centrally acting dextrorotatory analog of codeine, and its metabolite dextrorphan are also high-affinity non-competitive NMDA-channel blockers that are neuroprotective in experimental focal ischemia (Ginsberg, 1995
Aptiganel (CNS-1102, Cerestat) is the only non-competitive NMDA antagonist to have progressed to later-phase clinical trials. In pre-clinical reports, CNS-1102 treatment begun 15 min after MCA occlusion in rats yielded significant reductions in infarct volume by diffusion-weighted MR imaging and post mortem analysis (Minematsu et al., 1993
) and benefitted both gray and white matter (Schabitz et al., 2000
). The effective pre-clinical doses of ~2000 micrograms/kg were vastly higher than those tested in subsequent clinical trials (see below).
Clinical stroke trials
In the case of dextrorphan and MK-801, clinical development was halted in the pilot phase. In an ascending-dose trial of dextrorphan begun within 48 hours in patients with mild-to-moderate hemispheric stroke, a variety of dose-related reversible adverse events commonly occurred, including nausea and vomiting, somnolence, hallucinations, agitation, and rapid-onset symtomatic hypotension. The highest infusion rates induced deep stupor or apnea (Albers et al., 1995
). The development of MK-801 was also abandoned after early-phase clinical trial experience; although these results were never published, it is commonly assumed that MK-801 also induced dose-limiting neuropsychological adverse events (Olney, 1994
When aptiganel was tested in normal volunteers, the maximal tolerated dose was ~30 micrograms/kg i.v. over 15 min. (This dose-range, it should be noted, is several orders of magnitude below the effective neuroprotective doses shown in animals.) At higher doses, hypertension and unacceptable degrees of sedation and CNS excitation developed. Euphoria, disinhibition, hallucinations, psychomotor retardation, paranoia, and catatonia were described (Muir et al., 1994
; Muir et al., 1995
). These results notwithstanding, a phase II/III randomized controlled trial was begun: The Aptiganel Acute Stroke Trial was a double-blind trial of 628 patients at 156 centers randomized to treatment within 6 hours with high- or low-dose aptiganel or placebo (Albers et al., 2001
). The primary outcome was the modified Rankin score at 3 months. No improvement in the primary outcome was seen with either dose of aptiganel, and the 4-month mortality rate was higher
in the high-dose aptiganel group (26%) than in the placebo group (19%). The trial was aborted by the sponsor and by the independent data and safety monitoring board because of both a lack of efficacy and the imbalance in mortality.
Competitive NMDA antagonism
A single agent of this type – CGS 19755 (selfotel) -- was both evaluated pre-clinically (albeit in a rather limited fashion) and progressed to a larger clinical trial. In permanent MCA occlusion in rats, CGS 19755 administered within 5 min of the insult reduced infarct size and reduced postischemic glucose hypermetabolism, which was putatively excitation-induced (Simon et al., 1990
). In cats with 90-min MCA occlusion plus only 4 h of reperfusion, CGS 19755 in a dose of 40 mg/kg at 75 min led to ~50% infarct size reduction (Miyabe et al., 1997
). When this agent was brought to clinical trial, a small safety and tolerability study showed that adverse events, comprising agitation, hallucinations, confusion, paranoia, and delirium occurred in all
patients treated at 2 mg/kg and in a substantial fraction treated at lower doses (Grotta et al., 1995
). (One notes that this dose is far below the protective dose-range demonstrated pre-clinically.) Two pivotal phase 3 prospective studies were then carried out, in which 567 subjects were randomized to a single 1.5 mg/kg dose of selfotel within 6 hours of stroke onset (Davis et al., 2000
). The trials were suspended because of a trend toward higher mortality in the selfotel-treated group (Davis et al., 1997
; Davis et al., 2000
Several obvious factors emerge from the results presented above: a) Virtually all pre-clinical studies suggested that NMDA antagonists protected in focal ischemia only with very early administration
, yet the design of clinical trials incorporated much longer times to treatment. b) It was impossible to approximate the pre-clinically protective doses in clinical trials because of dose-limiting neurobehavioral toxicity in humans. In this context, a pre-clinical study is of interest, in which the therapeutic ratio (the ratio of the minimum effective dose for impaired rotarod performance and the minimum effective dose for significant neuroprotection) was measured in a mouse permanent-MCA occlusion model (Dawson et al., 2001
). For both aptiganel (Cerestat) and selfotel, this ratio proved to be very low
(1 or less), predicting the implausibility of clinical application. Finally, c) the possibility exists that NMDA antagonism, while potentially protective in focal ischemia, is also deleterious in terms of adversely affecting endogenous NMDA-receptor-mediated neuronal-survival mechanisms (Hoyte et al., 2004
NMDA receptor glycine-site antagonism: gavestinel
Glycine is a co-agonist at the NMDA receptor complex and is required for NMDA receptor function. GV150526 (gavestinel; (E)-3[(phenylcarbamoyl)ethenyl]-4,6-dichloroindole-2-carboxylic acid sodium salt) is a highly potent, highly selective antagonist of the glycine site.
Pre-clinical ischemia studies
In rats with MCA occlusion, GV150526, 3 mg/kg i.v., administered up to 6 h after occlusion yielded significant reduction of infarct volume measured at 24 h and protected somatosensory evoked potential (SEP) responses, although SEP responses recorded from a cortical field closest to the ischemic core were significantly protected only when treatment was begun at 1 h (Bordi et al., 1997
). In an MRI study in rats with MCA occlusion, although maximal protection was seen with pre-ischemic administration, when GV 150526 was first administered at 6 h, ischemic volume was still reduced by ~45% (Reggiani et al., 2001
). In contrast to NMDA receptor antagonists such as dizocilpine, GV 150526 failed to produce neuronal vacuolization or impaired learning, suggesting that it had a much greater margin of safety than other NMDA antagonists (Bordi et al., 1999
Clinical stroke trials
Several smaller clinical trials of gavestinel revealed a benign safety profile (Dyker et al., 1999
; North American GAIN Investigators, 2000
). Two pivotal randomized, double-blind multicenter phase III trials were then conducted: the GAIN Americas Trial (1,646 subjects) (Sacco et al., 2001
), and the GAIN International Trial (1,804 subjects) (Lees et al., 2000
). These two trials were similarly designed: Patients were stratified by age and stroke severity and were randomly assigned to treatment within 6 hours with either intravenous gavestinel (loading dose 800 mg, plus 5 doses of 200 mg each at 12-h intervals) or placebo. The primary outcome measure was the trichotomized Barthel index at 3 months. Although patients with acute intracranial hemorrhage were enrolled, they were excluded from the target population for the primary efficacy analysis. It is of interest that while tPA was administered in 24.4% of the primary efficacy population in the GAIN Americas Trial, none of the GAIN International subjects received it. Each of these trials was entirely neutral – i.e., showed no difference between treatment groups for either the primary outcome or for mortality or any secondary outcome measures (Lees et al., 2000
; Sacco et al., 2001
). A small MRI substudy drawn from these two trials, designed to test whether gavestinel would attenuate lesion growth relative to placebo, was also negative (Warach et al., 2006
). The patients from the GAIN International and GAIN Americas trials with intracerebral hemorrhage (N=571) were pooled for separate analysis, which revealed that gavestinel neither harmed nor benefited this subgroup (Haley, Jr. et al., 2005
In the case of gavestinel, we have an agent whose clinical
evaluation was thorough and exemplary in many respects: two large, independently conducted, well-designed, adequately powered phase III trials employing a clinically relevant primary outcome and drug-dosing that yielded plasma concentrations exceeding target levels for pre-clinically predicted protective efficacy (Lees et al., 2000
). Rather, it is the pre-clinical
evaluation that is found wanting. The demonstration of pre-clinical efficacy consisted of only a few published studies, mostly from Glaxo-Wellcome itself, whose methodological quality was sub-optimal (van der Worp et al., 2005
) and whose results were not independently replicated (Gorelick, 2000
AMPA receptor antagonism
It is of interest that, while AMPA antagonists showed neuroprotective efficacy in certain pre-clinical settings of both focal and global cerebral ischemia (Li et al., 1993
; Gill, 1994
; Xue et al., 1994
; Lees, 2000
; Takahashi et al., 2002
), the successful completion of larger clinical trials has not been reported. In a phase II double-blind multicenter trial (Elting et al., 2002
), the AMPA antagonist ZK200755, when administered in a higher dose, produced a marked transient worsening of the NIHSS score attributable to a depression of consciousness (stupor or coma). In addition, this agent elevated serum concentrations of S-100B (a glial marker). The trial was suspended after 61 patients for safety reasons, and further development ceased.
The ARTIST MRI Trial (AMPA Receptor Antagonist Treatment in Ischemic Stroke) was a randomized double-blind study of the AMPA antagonist YM872 in ischemic stroke, with a 6-hour treatment window, requiring a baseline MRI showing diffusion-perfusion mismatch. The primary outcome was MRI-lesion volume at day 28. This trial began in 2001 and as of July, 2002, had enrolled 108 patients (Internet Stroke Center, 2007
). A related ongoing trial, ARTIST+ , reported enrollment of 312 patients randomized within 3 hours to YM872 or placebo; all patients received i.v. tPA within 3 hours; efficacy measures were neurological function and disability scales (Internet Stroke Center, 2007
). No full-length reports have emerged from either trial.
GABA Agonism: Clomethiazole, Diazepam
Clomethiazole is a GABA(A)-agonist; its mechanism of action involves potentiation of the activity of GABA, the brain’s major inhibitory neurotransmitter (Wilby et al., 2004
). It has been widely used as a sedative.
Pre-clinical ischemia studies
Clomethiazole was perhaps more extensively evaluated in animal models of transient global forebrain ischemia than in focal ischemia. In global ischemia, its protective effect tended to disappear long-term and may have been mediated by hypothermia (Chaulk et al., 2003
). Focal ischemia studies included a study of 1-h MCA suture occlusion in rats, followed by 24-h reperfusion, in which clomethiazole was given either 60 min prior to
occlusion or at 10 min after the start of reperfusion (i.e., at 70 min after onset of ischemia) (Sydserff et al., 1995b
). Histological protection was observed. When studied in a rat model of permanent MCA occlusion, clomethiazole reduced the volume of ischemic damage by 58% when administered at 1 h after occlusion but was ineffective when given at 3 h (Sydserff et al., 1995a
). Clomethiazole was also studied in marmosets (small primates) with permanent MCA occlusion; treatment at 5 min reduced overall damage by 32% and improved use of the disabled arm (Marshall et al., 1999
). However, when treatment was initiated at 1 h after occlusion in this model, the agent improved spatial neglect but not the affected limb’s severe motor impairment (Marshall et al., 2000
Clinical stroke trials
In a safety and efficacy trial (the CLASS Trial) involving 1,360 patients with acute hemispheric stroke randomized to clomethiazole or placebo within 12 hours, no difference was found in the proportions of patients achieving functional independence at 90 days (Wahlgren et al., 1999
). However, in a subgroup with “total anterior circulation syndrome”, i.e., large hemispheric strokes, the clomethiazole-treated group had an ~11% higher proportion with functional independence than the placebo group. Sedation was the most common adverse event. A small pilot trial (CLASS-T) was then conducted to explore the effect of clomethiazole vs. placebo treatment within 12 hours, when added to conventional tPA therapy (Lyden et al., 2001
). The results again showed that patients with large hemispheric strokes treated with clomethiazole showed greater improvement than the placebo-treated group on the Barthel index at 90 days.
A subsequent large trial, CLASS-I, was designed to test the hypothesis, suggested by the earlier findings, that clomethiazole would be protective in larger ischemic strokes (Lyden et al., 2002
). That trial studied 1,198 patients with major acute ischemic stroke producing limb weakness, higher cortical dysfunction and visual field deficits. Subjects were randomized to clomethiazole or placebo beginning at 12 hours. The proportion of patients attaining a Barthel index score of 60 or above at 90 days did not differ between groups. Subgroup analysis of patients treated early (< 6h) versus 6–12 hours also failed to show a treatment effect.
Clomethiazole was also studied in a randomized trial of neuropsychological function after coronary artery bypass surgery (245 pts), with treatment administered during surgery. No improvement was shown (Kong et al., 2002
Pre-clinical studies showed that the protective effect of clomethiazole depended upon its very early administration, yet the clinical trials for acute stroke allowed a time-to-treatment of 12 hours. Their negative outcomes are not surprising.
, a GABA-ergic drug, was studied in a trial of 880 stroke patients randomized within 12 hours of onset. The primary outcome - independence on the Rankin scale at 3 months - was not significantly different between groups (Lodder et al., 2006
Magnesium may be viewed as an endogenous calcium antagonist that may protect via multiple mechanisms, including NMDA receptor blockade, inhibition of excitatory neurotransmitter release, blockade of calcium channels, as well as vascular smooth muscle relaxation (Ovbiagele et al., 2003
). Magnesium sulfate has long been used clinically with great success to treat pre-eclampsia, where it reduces the risk of frank (convulsive) eclampsia by more than one-half (Witlin et al., 1998
; Duley et al., 2003
Pre-clinical ischemia studies
Studies of magnesium as a neuroprotectant in focal ischemia have been rather limited in scope. In one study, intraperitoneal MgCl2
administered just after MCA occlusion in rats reduced infarct volume in rats (Izumi et al., 1991
). In another study in rats with autologous MCA thromboemboli treated with 5% MgSO4
(90 mg/kg), improved neurological outcome was described with administration out to 6 h (Yang et al., 2000
). A third study, designed primarily to assess serum levels, treated rats with 90-min MCA occlusion beginning with a pre-ischemic
bolus; magnesium serum levels below 3 mmol/L were well tolerated but doses above that level produced cardiodepressive effects. Mean infarct-volume reductions of up to 42% were observed (Westermaier et al., 2005
). A fourth study employed pre-ischemic intracarotid
infusion of MgSO4
prior to MCA occlusion in rats; with higher Mg doses and shorter ischemia durations, infarct-volume reductions up to ~60% could be achieved (Marinov et al., 1996
). The overall impression in a recent review of these trials was of mixed results, and concern that a possibly confounding (i.e., protective) influence of hypothermia could not be excluded in the positive studies (Meloni et al., 2006
Clinical stroke trials
A large multicenter efficacy trial was conducted (Intravenous Magnesium Efficacy in Stroke Trial; IMAGES) in 2,589 patients with acute stroke, in which subjects were randomized to intravenous MgSO4
or placebo with a 12-hour window to treatment (Muir et al., 2004
). The primary outcome was a global endpoint incorporating death or disability at day 90; this outcome failed to be improved by magnesium, and mortality was slightly higher in the magnesium- than in the placebo-group. Secondary outcomes were also negative, but planned a subgroup analysis showed a benefit of magnesium in non-cortical strokes.
Magnesium is a potentially appealing neuroprotectant because it is safe and inexpensive. Nonetheless, the pre-clinical studies reviewed above reveal that it is, in fact, a rather weak neuroprotectant. As is typical of trials with other agents, the multicenter IMAGES trial incorporated a window-to-treatment (12 h) vastly longer than was justified by the findings of pre-clinical focal-ischemia studies. In IMAGES, the median time-to-treatment was 7 hours, and only 3% were treated within 3 h (Muir et al., 2004
Antioxidant Spin-Trap: NXY-059
NXY-059 (disodium 4-[tert-butylimino)methyl] benzene-1,3-disulfonate N-oxide), a proprietary nitrone spin-trap agent, is a bis-sulfonated derivative of PBN [α-phenyl-N-tert-butyl nitrone], a generic nitrone spin-trap agent that has been shown to protect in models of brain ischemia and trauma.
Pre-clinical ischemia studies
In a transient (2-h) MCA occlusion model in rats, NXY-059 treatment begun at 1 hour after recirculation had a marked dose-dependent effect on infarct volume, with the highest dose reducing infarct volume by 77% (Kuroda et al., 1999
). Even when started at 3 h of recirculation (i.e., 5 h after the onset of ischemia), neurologic deficits were still improved and infarct volume was still reduced by approximately two-thirds. Other workers confirmed these effects in similar models (Sydserff et al., 2002
) Neuroprotective effects were also described in rat models of permanent
MCA occlusion: In one such study, higher-dose NXY-059 treatment begun at 5 min after onset of permanent MCA occlusion in spontaneously hypertensive (SHR) rats led to a 36% reduction in cortical infarct volume (Zhao et al., 2001
). In another permanent-occlusion model in rats, 44% infarct reduction was observed even when treatment was initiated at 4 h after stroke onset (Sydserff et al., 2002
In marmosets pre-trained on behavioral tasks and subsequently given permanent MCA occlusion, NXY-059 treatment begun at 5 min and continued for 48 hours led to improved function of the hemiparetic arm and a lessening of spatial perceptual neglect at 3 and 10 weeks, and to 51% reduction in overall brain damage (Marshall et al., 2001
). When treatment was begun at 4 hours after stroke onset, behavioral improvement and a 28% reduction in the overall infarct still resulted (Marshall et al., 2003
). Beneficial effects of NXY-059 were also reported in a small-clot embolus model in rabbits (Lapchak et al., 2002b
). In similar studies employing large-clot embolism, NXY-059 used alone increased
the brain-hemorrhage rate but, when used together with tPA, appeared to reduce tPA-induced hemorrhage (Lapchak et al., 2002a
The pre-clinical development of NXY-059 was more extensive than for most other candidate-drugs: The agent was tested in both transient and permanent models of focal ischemia and in both rodents and non-human primates; physiological monitoring was generally appropriate. Both behavioral and histological end-points were used. Beneficial results were replicated in several laboratories. Nonetheless, various deficiencies of these studies have been identified (Savitz, 2007
). However, when taken together these pre-clinical results were certainly promising enough to warrant consideration of clinical trials. The high safety-profile of NXY-059 allowed plasma levels to be achieved in humans that equaled or exceeded levels shown to be effective in animal models (Green et al., 2003
Nonetheless, certain shortcomings are evident in the properties of the compound itself: NXY-059 is a polar, highly water-soluble molecule with very low blood-brain barrier (BBB) permeability (Kuroda et al., 1999
) and negligible cellular penetration (Dehouck et al., 2002
), suggesting that its major action might be confined to the blood-endothelial cell interface. In addition, NMR-based studies have shown that both PBN and NXY-059 are two orders of magnitude less
potent in inhibiting free radical-mediated peroxidative reactions than physiological compounds such as vitamin E (Becker et al., 2002
); and cyclic voltametry reveals that PBN (and by inference, NXY-059) have oxidation potentials far higher than those of important biological chain-breaking antioxidants such as vitamin E, glutathione, and beta-carotene (Buettner, 1993
; Becker et al., 1998
; Ginsberg, 2007
). Finally, NXY-059 has extremely low potency, so that very large quantities must be administered.
Clinical stroke trials
NXY-059 was studied in two large randomized, double-blind trials, which to some extent proceeded concurrently. The first trial, SAINT I, involved 1,722 patients (Lees et al., 2006
); SAINT II enrolled 3,306 subjects (Shuaib et al., 2007
). In both trials, subjects were randomly assigned to receive a 72-hour infusion of intravenous NXY-059 or placebo beginning within 6 hours of stroke onset; each study site was required to maintain an average time-to-treatment of 4 hours or less. In SAINT I, NXY-059 significantly improved the overall distribution
of scores on the modified Rankin scale (mRS) as compared to placebo (p=0.038), with a common odds ratio of 1.20 (95% CI, 1.01–1.42) (Lees et al., 2006
). However, NXY-059 did not improve outcome on the NIHSS scale or the Barthel index. By contrast, the results of SAINT II were entirely negative
: the distribution of mRS scores was virtually identical in the NXY-059 and placebo groups, and there was no evidence of efficacy for the secondary end-points (Shuaib et al., 2007
). As regards safety, an excess of hypokalemia was noted in NXY-059-treated patients (Serebruany, 2006
Why did the results of SAINT I and II differ? Closer inspection of the SAINT I results reveals that, despite a statistically significant
treatment effect on the overall mRS distribution, the magnitude of clincal effect
was in fact quite modest and, it might be argued, unimportant. For example, if one were to use a mRS score of 0–1 as a more stringent definition of favorable outcome, the NXY-059 and placebo groups of SAINT I would have attained this result in 33.4% and 31.0% of subjects, respectively – i.e., an absolute percentage difference of only 2.4% and a predicted needed-to-treat number of 41.7 to achieve one positive outcome (Ginsberg, 2007
). In this author’s view, the “positive” results of SAINT I are likely to have occurred by chance. Other authors have critically analyzed the design- and statistical weaknesses of SAINT I (Koziol et al., 2006
; Saver, 2007
). One curious design feature was the inclusion of both tPA-treated and non-tPA-treated subjects (tPA fraction, 29% in SAINT I, 44% in SAINT II) without adequately powering these subgroups for analysis of the primary outcome (Lees et al., 2006
; Shuaib et al., 2007
). It is conceivable that NXY-059 with vs. without tPA might have conferred differential benefit.
Others have been quick to draw pessimistic generalizations from the failure of SAINT II (.e.g., “ The failure of SAINT II… raises a number of questions about the future of neuroprotection trials and casts doubt on the neuroprotection hypothesis”) (Savitz et al., 2007
). The latter inference appears strongly counter-intuitive given the abundant experimental evidence that neuroprotection can, in fact, be achieved. Rather, attention should be directed primarily to the characteristics of the compound itself. Factors likely to have contributed to the lack of clinical success with NXY-059 are: a) its sub-optimal biochemical and physicochemical profile (poor CNS penetration, poor redox characteristics) and b) the fact that SAINT I and II utilized longer windows to treatment (6 hours) than had been shown to confer efficacy when NXY-059 was studied experimentally.
Other Antioxidants: Tirilazad, ebselen, edaravone
Three other antioxidant/radical scavenging agents also deserve mention because each has undergone clinical trials of over 200 subjects.
(U-74006F) is a non-glucocorticoid 21-aminosteroid inhibitor of iron-dependent lipid peroxidiation that was extensively evaluated pre-clinically beginning in the mid-1980’s in models of traumatic brain injury, hemorrhagic shock, spinal cord injury, experimental subarachnoid hemorrhage, cardiopulmonary arrest, excitotoxicity, as well as in global and focal cerebral ischemia. An extensive systematic pre-clinical review and meta-analysis has been published, surveying animal models of focal ischemia involving tirilazad in which outcome was measured as infarct volume and/or neurological score (Sena et al., 2007
). Overall, tirilazad reduced infarct volume by 29% (95% CI, 21–37%) and improved neurobehavioral score by 48%. Efficacy was higher in temporary occlusion than in either permanent or thrombotic models (Sena et al., 2007
). Maximum efficacy was seen when treatment was given before
the onset of ischemia, with a trend for efficacy to decline thereafter. It is noteworthy that the median time to administration in the studies surveyed was only 10 min
after onset of the insult! The authors’ analysis reveals that, with times to treatment greater than 60 min, the efficacy data showed very broad confidence intervals (Sena et al., 2007
). Furthermore, tirilazad was effective over a very narrow dose range of 3–10 mg/kg.
A prospective, randomized clinical trial of tirilazad mesylate was conducted in 660 patients with acute stroke treated within 6 hours of onset (median 4.3 h); the primary outcome was disability on the Glasgow Outcome Scale and Barthel index at 3 months (The RANTTAS Investigators, 1996
). The trial was prematurely terminated based on an independent interim analysis. In the 556 fully analyzed subjects, there was no effect of tirilazad on outcome. A radiological analysis of these subjects revealed that tirilazad had no effect on infarct volume (van der Worp et al., 2002
). A subsequent clinical trial, RANTTAS II, was planned to test the hypothesis that the lack of efficacy in RANTTAS might have been secondary to inadequate dosing. The trial was stopped when questions regarding safety emerged from a parallel trial in Europe (Internet Stroke Center, 2007
). A Cochrane-Collaboration meta-analysis was published of six double-blind placebo-controlled tirilazad trials (4 published, 2 unpublished) in 1,757 patients with acute ischemic stroke (Tirilazad International Steering Committee, 2000
). This analysis revealed that, while tirilazad did not alter early or end-of-trial case fatality rates, it did increase the odds of being dead or disabled by about one-fifth (borderline-significant).
In comparing the pre-clinical and clinical tirilazad studies, it is apparent that the clinical trials a) used a much broader (by implication, too low) dose range and b) employed a much longer time to treatment (median ~5 h) than the animal trials (median, 10 min).
(2-phenyl-1,2-benzisoselenazol-3(2H)-one) is a selenium compound with glutathione peroxidase-like activity; it also reacts with peroxynitrite and inhibits a variety of enzymes (Parnham et al., 2000
). In rodent models of MCA-distribution ischemia, ebselen reduced ischemic damage when administered prior to
ischemia (Namura et al., 2001
) and was modestly protective if begun 30 min after induction of ischemia (Takasago et al., 1997
). When treatment was begun at the onset of recirculation after 2-h MCA occlusion in rats, ebselen reduced early brain injury and improved neurological deficits at 24 hours (Imai et al., 2001
), but this protection was not sustained at 7 days (Salom et al., 2004
). In a clinical trial of 302 acute ischemic-stroke patients randomized to oral ebselen or placebo treatment begun within 48 hours and continued for 2 weeks, the intent-to-treat primary-outcome analysis was significant for ebselen at 1 month but not 3 months (Yamaguchi et al., 1998
). A secondary analysis suggested benefit in the ebselen-subgroup treated within 24 h. A Phase III study of 394 patients incorporating a 24-hour window was slated to begin in 2001 (Internet Stroke Center, 2007
), but no report has appeared.
(MCI-186; 3-methyl-1-phenyl-2-pyrazolin-5-one) is an oxygen radical scavenger and blocker of lipid peroxidation. Early pre-clinical reports described salutary effects in both global and focal cerebral ischemia (Watanabe et al., 1994
). In a rat modelf of transient (1-h) MCA occlusion, edaravone reduced infarct volume when administered prior to the insult but failed to protect when started at 1–2 hours after onset of ischemia (Nakajima et al., 2005
). Another study reported ~30% reduction of total infarct volume in rats with 2-h MCA occlusion treated at the onset of reperfusion (Amemiya et al., 2005
). In mice with 60-min MCA occlusion, edaravone appeared to reduce infarct volume and improve neurological score at 24 h even when administered at 6 h after onset of ischemia (Zhang et al., 2005a
). Only a single randomized clinical trial of edaravone has been fully published: a phase II study of 252 ischemic-stroke patients with a 72-hour window to treatment; this trial was said to show improved outcome on the modified Rankin scale at 3 months (Edaravone Acute Infarction Study, 2003
Phospholipid Precursor: Citicoline
Citicoline refers to the exogenously supplied form of cytidine 5-diphosphocholine (CDP-choline), a product of the rate-limiting step in the synthesis of phosphatidylcholine from choline. Orally administered citicoline is hydrolyzed in the gut to cytidine and choline, which are rapidly absorbed (Weiss, 1995
), cross the blood-brain barrier, and can be incorporated into the phospholipid fraction of neuronal membranes (Secades et al., 1995
). CDP-choline increases phospholipid synthesis, inhibits phospholipid degradation and free fatty acid release, increases CNS levels of norepinephrine and dopamine, and restores mitochondrial and membrane ATPase activities (Secades et al., 1995
; Weiss, 1995
). While CDP-choline and its components do not directly affect phospholipase A2 (PLA2) activity in vitro, when studied in vivo citicoline attenuates ischemia-induced PLA2 stimulation and thereby diminishes the injurious consequences of phospholipid hydrolysis – namely, the generation of arachidonic acid, whose metabolism leads to formation of reactive oxygen species, lipid peroxides and toxic aldehydes (Adibhatla et al., 2003
). In the setting of transient focal ischemia, citicoline restores phosphatidylcholine levels by differentially affecting PLA2 and CTP-phosphocholine cytidlyltransferase (Adibhatla et al., 2006
). Citicoline also inhibits glutamate-induced apoptosis in cultured cerebellar granule neurons (Mir et al., 2003
) and increases glutamate uptake and expression of the membrane glutamate transporter EAAT2 in cultured astrocytes (Hurtado et al., 2005
Pre-clinical ischemia studies
Several studies in rodent models of focal ischemia have been reported. In a rat model of transient MCA occlusion, high-dose citicoline treatment begun at the time of reperfusion (2 h) reduced infarct volume by one-half, but the study was flawed by failure to control brain temperature and substantial premature mortality (Schabitz et al., 1996
). In another MCA occlusion study, treatment was begun at 15 min after onset of ischemia; citicoline improved behavioral and morphological indices chiefly in rats with submaximal insults (ischemia of 30–75 min) (Aronowski et al., 1996a
). When ischemia was produced by clot embolus, one study showed that citicoline begun at 45 min failed to affect infarct size unless tPA was co-administered (Andersen et al., 1999
); and another showed that citicoline administered prior to tPA failed to confer additional benefit but, if administered 10 min after
tPA, led to less ischemic injury than with tPA alone (Alonso de Lecinana M. et al., 2006
). An interesting recent report (Hurtado et al., 2007
) provides evidence that citicoline treatment begun 24 hours after MCA occlusion and maintained for 28 days improves functional outcome and enhances dendritic complexity and spine density in layer V pyramidal cells of the undamaged motor cortex, suggesting that chronic treatment may increase neuronal plasticity within noninjured, functionally connected brain regions.
The biochemical actions of exogenous CDP-choline (citicoline) have been well characterized. However, the published reports of citicoline in pre-clinical ischemia models were limited in scope and failed to provide key translational data needed to assess the potential for clinical neuroprotection (particularly, defining the therapeutic window of efficacy). Positive results were linked to early administration. The interesting possibility that chronic citicoline treatment enhances neuronal plasticity deserves further study.
Clinical stroke trials
Citicoline in doses of 1 g per day for 14 days proved entirely safe (Tazaki et al., 1988
). Indeed, the clinical safety of citicoline has been repeatedly confirmed. Four important trials and a pooled meta-analysis have been reported. In the first, 259 acute ischemic stroke patients were randomized to 6-week treatment with citicoline (3 different doses) or placebo commencing within 24 hours (mean, 14.5 h). With covariate adjustment for baseline NIHSS scale, 2 citicoline dose-groups were found to be significantly improved at 90 days on the Barthel index (Clark et al., 1997
). There ensued a 33-center randomized double-blind efficacy trial in 394 subjects, with oral treatment begun within 24 hours and continued for 6 weeks (Clark et al., 1999b
). There was a significantly higher percentage of patients with mild stroke (baseline NIHSS < 8) in the placebo group compared to the citicoline group. The primary efficacy analysis (Barthel index at 12 weeks) was not completed due to the failure of a key statistical assumption, and the planned secondary analyses were not positive. Only a post-hoc analysis of the subgroup with baseline NIHSS = > 8 suggested that citicoline subjects were more likely to have full recovery (33%) than placebo (21%). A third trial in 899 ischemic-stroke subjects treated beginning at 24 hours (mean, 13 h) again showed no between-group difference in the primary analysis (Clark et al., 2001
). A fourth study was an MRI-based trial of 100 acute ischemic stroke subjects treated for 6 weeks, beginning within 24 h, and followed for MRI lesion-size changes at 12 weeks (Warach et al., 2000
). The primary MRI analysis (baseline-to-12-week change) showed no significant difference between groups, but secondary analysis of lesion volume from week 1 to week 12 showed a greater decrease in the citicoline group.
A pooled analysis of individual patient data from the 4 randomized trials of oral citicoline described above (N=1,372 with baseline NIHSS = >8), re-analyzed using a global 3-month outcome measure incorporating NIHSS, mRS and Rankin scales, revealed a statistically significant effect of citicoline on global recovery (25.2% vs. 20.2%, OR 1.33 (95% CI, 1.10–1.62, p=0.0034) (Davalos et al., 2002
). The highest effect was seen in the 2000 mg dose-group.
Citicoline is consistently safe in patients. A key flaw of all these trials is their unrealistically long time to treatment – 24 hours – a duration adopted despite a complete absence of pre-clinical evidence supporting efficacy with a 24-h delay to treatment. Individual clinical trials suffered from other design flaws. The significant result of the pooled meta-analysis is, however, a “positive” result and is consistent with either a mild neuroprotective effect or else an effect of citicoline on longer-term neuronal plasticity and recovery processes. A major strength is that all these studies were published in full. Currently ongoing is the ICTUS International Citicoline Trial on Acute Stroke; planned recruitment is N=2,600; combined neurological and MRI end-points will be considered. It is unfortunate that the window to treatment remains at 24 hours
(Internet Stroke Center, 2007
Nitric Oxide Signal Transduction Down-Regulator: Lubeluzole
Lubeluzole, the S-isomer of a novel 3,4-difluoro benzothiazole, is thought to act by down-regulating the glutamate-activated nitric oxide synthase pathway (Lesage et al., 1996
). In vitro, lubeluzole improved the ability of hippocampal slices to recover their membrane potential after repeated DC depolarizations (Ashton et al., 1997
) and protected hippocampal neurons against nitric oxide toxicity (Maiese et al., 1997
Pre-clinical ischemia studies
Lubeluzole was studied most extensively in a photochemical model of parietal sensorimotor cortical stroke in rats; in this model, lubeluzole rescued tactile/proprioceptive hindlimb placing reactions when administered 5 min after stroke onset and protected 60% of rats when administered at 6 h (De Ryck et al., 1996
). The optimal regimen begun at 5 min reduced infarction by 28% at 7 days (De Ryck et al., 1996
) and attenuated infarct growth as assessed by MRI (De Ryck et al., 2000
). Lubeluzole also blocked peri-infarct glutamate increases in this model (Scheller et al., 1997
). In rats with reversible MCA and carotid artery occlusions for 120 min or more, lubeluzole treatment started 15 or 30 min after onset of ischemia reduced infarct size by 50% and 34%, respectively (Aronowski et al., 1996b
). Other workers described infarct-volume reductions of 33% in rat MCA occlusion with treatment begun at 3 h (Culmsee et al., 1998
). In rats with global cerebral ischemia, lubeluzole treatment begun at 5 min protected hippocampal CA1 neurons (Haseldonckx et al., 1997
These pre-clinical results support mechanistically based neuroprotective efficacy with very early administration but do not adequately substantiate whether robust neuroprotection is possible with longer, clinically relevant, treatment delays.
Clinical stroke trials
A safety study in 193 patients with carotid artery-territory ischemic stroke was terminated prematurely because of an imbalance in mortality between treatment groups, highest in the 20 mg/day lubeluzole group; the lower, 10 mg/day dose was considered safe (Diener et al., 1996
). A multicenter US and Canadian double-blind trial was then conducted in 721 patients randomized to lubeluzole (7.5 mg + 10 mg/day × 5 days) or placebo initiated within 6 hours of symptom onset. The primary end-point, mortality at 12 weeks, did not differ between groups, but recovery on the NIHSS scale and Barthel index was improved by lubeluzole (Grotta, 1997
). A European and Australian study randomized 725 patients to treatment in a manner similar to the US and Canadian study, with a 6-hour treatment window. Overall mortality at 3 months was again similar for lubeluzole and placebo, but a post-hoc analysis suggested that mortality in patients with mild or moderate strokes was reduced by lubeluzole (Diener, 1998
). Finally, a third large trial was then carried out in 1,786 patients, with the efficacy analysis conducted in patients 75 years old or younger treated at 0–6 hours after stroke. The treatment groups showed no difference in either the primary outcome measure -- the trichotomized Barthel index at 12 weeks -- or secondary outcome measures (Diener et al., 2000
). A Cochrane Database meta-analysis, reviewing five trials and 3,510 patients, also found no effect on mortality or dependency but noted a significant increase of heart-conduction disorders (Q-T prolongation) in lubeluzole-treated subjects (Gandolfo et al., 2002
These studies mostly used a 6-h window to treatment – a duration not strongly supported by the pre-clinical data. It might be argued that the extent of preclinical efficacy was insufficient to warrant such a large investment in clinical trials. The use of mortality as a primary outcome measure is considered to be a weak design feature; in current trials, neurological outcome and functional recovery are preferred as primary end-points.
Leukocyte Inhibition: Enlimomab and UK 279,276
is a murine intercellular adhesion molecule-1 (ICAM-1) antibody that reduces leukocyte adhesion. In rats with 2-h transient MCA occlusion treated at 1 h of reperfusion, a reduction in size of the ischemic lesion was seen; there was no effect, however, in permanent occlusion (Zhang et al., 1995
). In the EAST multicenter clinical trial, 625 patients with acute ischemic stroke were randomized to enlimomab or placebo within 6 hours of onset. The primary efficacy endpoint was the modified Rankin Scale at 90 days. This trial showed a highly significant worsening of outcome
in enlimomab-treated patients compared to placebo (p=0.004), and a higher death rate (EAST Trial, 2001
). To elucidate the reasons for this negative clinical outcome, an experimental study was performed in which a murine anti-rat ICAM-1 antibody was administered to rats with focal ischemia (Furuya et al., 2001
). This treatment failed to reduce infarct size but elicited host antibody production and activated circulating neutrophils and complement; these mechanisms were offered as a possible explanation for the treatment-related deterioration in the clinical trial.
In a related approach, neutrophil activation was blocked by a recombinant protein inhibitor of the CD11b/CD18 receptor, UK 279,276
. In the ASTIN Trial (Acute Stroke Therapy by Inhibiton of Neutrophils), 966 acute stroke patients were treated within 6 hours of onset with the agent or placebo, and neurological recovery was assessed at 90 days. The trial, which incorporated a Bayesian sequential design, was terminated early for futility (Krams et al., 2003
). A possible reason to explain lack of efficacy was a body of pre-clinical evidence that UK 279,276 conferred benefit in reperfused
focal ischemia models but failed in the absence of reperfusion (Sughrue et al., 2004
Hemodilution is thought to decrease blood viscosity and may increase cerebral perfusion or oxygen delivery, but in fact various hemodiluting agents have differing effects. For example, in a comparison study (Haass et al., 1986
), both dextran 40 and hydroxyethyl starch (HES) lowered hematocrit and whole-blood viscosity, but dextran 40 increased
plasma viscosity and red-cell aggregration while HES did the opposite. Thus, HES was thought to be more appropriate for use in plasma volume-expansion.
The literature contains many examples of small clinical trials of hemodilution for stroke (Asplund, 2002
); these will not be reviewed here. The larger hemodilution trials in acute ischemic stroke form a highly heterogeneous group, employing a variety of agents, procedures, and settings. The Scandinavian Stroke Study was a randomized phase III trial of 373 subjects with a 48-hour treatment window; venesection and dextran 40 were both employed. There was no improvement in outcome or survival at 3 months (Scandinavian Stroke Study Group, 1987
), and a subgroup analysis was also negative (Scandinavian Stroke Study Group, 1988
). In a large Italian trial, 1,267 patients with hemispheric stroke (comprising both ischemia and intracerebral hemorrhage) of 12 hours or less, and with hematocrit of 35% or more, were randomized to hemodilution by venesection and dextran 40 volume replacement, or to control management (Italian Acute Stroke Study Group, 1988
). Mortality at 6 months was similar in the two groups, and there was no improvement with treatment even in the subgroups with ischemic stroke < 6 h and highest hematocrit. In another trial, the Amsterdam Stroke Study (Goslinga et al., 1992
), 300 patients were studied. Normovolemic hemodilution was induced with 20% albumin plus crystalloids, targeting a pulmonary capillary wedge pressure of 12 mmHg and hematocrit of 32%. Analysis at 3 months suggested a complex pattern of responses related both to treatment and to initial hematocrit. In the IASS-H trial, 502 patients were randomized with respect to both GM1 ganglioside treatment and hemodilution, with a 12-hour window; the results were negative (Argentino et al., 1989
). Finally, the Multicenter Austrian Hemodilution Trial was a randomized double-blind study of hypervolemic hemodilution instituted within 6 hours of MCA-territory ischemic stroke; groups were randomized to either 10% hydroxyethyl starch or to pure rehydration with Ringer’s lactate (Aichner et al., 1998
). The primary outcome was clinical improvement within 7 days on the Glasgow Outcome Scale. The trial was halted after an interim analysis of 200 patients failed to show a beneficial effect.
A Miscellany of Other Agents Studied Without Success in Larger Clinical Trials
These agents are listed in descending order of numbers of subjects studied.
, a fluoro-oxindole, is an activator of neuronal potassium channels. In rats with permanent occlusion, BMS showed ischemic protection with treatment at 2 hours (Gribkoff et al., 2001
). This compound was studied in two parallel clinical trials involving 1,978 subjects randomized to treatment within 6 hours of stroke onset. The primary outcome was change from baseline to 12 weeks on the NIHSS scale. The agent failed to exhibit efficacy, and a full publication did not appear (Internet Stroke Center, 2007
, a monosialoganglioside, was studied in a trial of 287 acute stroke patients randomized to treatment within 48 hours with GM1 by intramuscular injection, or placebo, for 28 days. The trial was negative (SASS Trial, 1994
). In another trial (also mentioned above), 502 acute stroke patients were randomized to treatment initiated within 12 hours with GM1 or placebo, with or without hemodilution. Again, there was no effect of treatment (Argentino et al., 1989
). In a third randomized trial of GM1, 792 subjects were treated within 5 hours. The primary outcome was mortality and change in Canadian Neurological Scale score at 4 months. There was a non-significant trend toward greater improvement with GM1 (Lenzi et al., 1994
). A Cochrane meta-analysis found that there was insufficient evidence to conclude benefit of GM1 in stroke, and caution was warranted because of sporadic cases of Guillain-Barre syndrome after ganglioside therapy (Candelise et al., 2001
is an agent thought to act as a modifier of membrane fluidity (Muller et al., 1999
), cognition- and microcirculation-enhancer, neuroprotectant, and anticonvulsant (Winblad, 2005
). A phase III trial of piracetam was conducted in 927 acute stroke patients randomized to treatment within 12 hours of onset. The primary outcome, neurological function at 4 weeks, was similar in the two groups, but post-hoc analyses suggested superiority of piracetam in the subgroup presenting within 7 h of onset (De Deyn et al., 1997
, an opioid antagonist with relative selectivity for kappa opiate receptors, received very little experimental testing in animal models of focal ischemia but was nonetheless brought to clinical trial. A phase II trial of 312 patients randomized to treatment within 6 hours revealed safety but no significant difference in functional outcome at 3 months (Clark et al., 1999a
). Similarly, a phase III trial of 368 subjects treated within 6 hours showed no difference in the primary outcome – Barthel index and Glasgow Outcome Scale at 12 weeks (Clark et al., 2000
Basic fibroblast growth factor
is thought to stabilize intracellular calcium ion homeostasis, induce antioxidant enzymes, and diminish glutamate-mediated excitotoxicity (Mattson, 1997b
). A randomized multicenter trial, planned for 900 subjects with a 6-hour treatment window, was halted after 286 subjects were enrolled. There was no significant difference in outcome between groups (Bogousslavsky et al., 2002
). Another similar trial was aborted after 302 subjects, and the results were not published.
, a sodium channel blocker and anticonvulsant, was studied in a phase III trial of 462 ischemic strokes randomized to treatment within 4 hours. The primary outcome was modified Rankin scale at 3 months. The trial was stopped after an interim analysis that found no differences between treatment groups. The results were not published (Internet Stroke Center, 2007
This calcium channel blocker was studied in a multicenter randomized trial of 331 subjects treated within 24 hours. There was no effect of treatment (Franke et al., 1996
, a vasodilator with possible rheologic effects, was studied in a phase II study of 297 subjects with acute stroke randomized to treatment within 12 hours. Followup was 28 days. The groups did not differ in mortality or functional deficit (Hsu et al., 1988
Repinotan (BAY x3072)
, a serotonin 5HT1A receptor agonist, was studied in a phase II trial of 240 subjects with hemispheric ischemia treated within 6 hours (Bayer Randomized Acute Ischemia Neuroprotectant Study – BRAINS). The results, which are assumed to have been negative, were not published (Internet Stroke Center, 2007
Shortcomings of Completed Clinical Trials of Neuroprotection
From the review of individual agents and trials presented above (Table 4
), many shortcomings, both pre-clinical and clinical, crop up repeatedly from agent to agent. Taken singly, each factor is potentially capable of sabotaging success; when present in combinations, failure is virtually assured. Some of these factors are more important than others. The discussion below highlights key considerations.
- Time-window to treatment a) There is practically no evidence that neuroprotection for acute ischemic stroke is possible with any agent beyond ~6 hours. b) Even within the 4–6 hour upper limit of putative efficacy, the extent of protection with any agent is likely to decline with increasing delay to administration. c) Whenever a clinical trial establishes a treatment-window of X hours, the majority of subjects are enrolled within the last possible hour. Taken together, these points force one to the conclusion that clinical trials of neuroprotection must be designed with a very early window to treatment. In over one-half of the completed trials reviewed here, the window was beyond 6 hours; and even a 6-hour window may have been too late. Furthermore, it is often the case that clinical trials were designed with a long window-to-treatment despite strong pre-clinical evidence that the window was, in fact, much shorter. Any hope for clinical success in these cases must be judged as purely fanciful.
- Solid pre-clinical evidence of neuroprotective efficacy: The minimal pre-clinical preconditions to be satisfied before bringing an agent to clinical trial should be: a) demonstration of robust, clinically relevant protective efficacy (e.g., 50% or greater infarct-size reduction plus neurobehavioral improvement with a window-to-treatment of at least 3–4 hours after onset of ischemia); b) proper experimental design of these studies (including full monitoring and control of physiological variables, so as to avoid (for example), the confounding influence of brain hypothermia; randomized allocation to treatment groups; blinded outcome assessment; demonstration of enduring protective effect with survival times of at least several days to one week; proper statistics with correction for multiple comparisons; etc.); and c) confirmation (replication) of positive findings by other laboratories. Many if not most of the large completed clinical trials reviewed above were launched without fully satisfying these pre-clinical milestones. Recent reviews critically addresses the factors that contribute to the quality of experimental stroke research (van der Worp et al., 2005; Dirnagl, 2006).
- Ability to achieve pre-clinically efficacious drug-doses or plasma levels in human subjects: There are several examples of clinical trials designed using drug-dosing vastly lower than the levels shown to protect the brain of animals. For some agents (e.g., NMDA antagonists), dose-limiting adverse events not fully anticipated in pre-clinical studies emerged during human trials. In general, agents that cannot be administered to humans in doses equivalent to those required for neuroprotection in animals should not be brought to phase II/III clinical trial, and agents with an unfavorable therapeutic ratio of efficacy relative to toxicity should probably be avoided.
- Incorporation of crucial design-features into pivotal clinical trials: These include: a) a short window to treatment compatible with the pre-clinical findings; b) drug-dosing compatible with pre-clinically demonstrated efficacious doses; c) adequate follow-up period (3 months is commonly used in ischemic stroke trials); d) choice of clinically meaningful primary-outcome measures and avoidance of statistically forgiving but clinically unimportant end-points; e) sample size powered to demonstrate efficacy with statistical certitude and avoid type II errors; f) choice of stratification variables relevant to the agent in question (e.g., thrombolysed vs. non-thrombolysed subjects -- a factor of importance with agents that require reperfusion in order to neuroprotect). Again, many of the completed trials discussed above were deficient in these respects (Cheng et al., 2004).